WO2019242009A1

WO2019242009A1 - Accurate micro liquid diluting and moving device, and liquid diluting and moving method

Info

Publication number: WO2019242009A1
Application number: PCT/CN2018/092440
Authority: WO
Inventors: 马云天; 马荣华
Original assignee: 江苏科华医疗器械科技有限公司
Priority date: 2018-06-19
Filing date: 2018-06-22
Publication date: 2019-12-26
Also published as: CN108786948A

Abstract

An accurate micro liquid diluting and moving device and a liquid diluting and moving method. The liquid diluting and moving device comprises an air bag I (1), a capillary tube I (4), a capillary tube II (2), and a sleeve (3); one end of the capillary tube II (2) is inserted into the air bag I (1); the other end of the capillary tube II (2) is inserted into the sleeve (3); the sleeve (3) is provided with an inner concave part (5); the upper part of the inner concave part (5) is connected with the lower port of the capillary tube II (2); the lower part of the inner concave part (5) is connected with the upper port of the capillary tube I (4). When in use, first suck up a sample liquid by means of the capillary action of the capillary tube I (4), then push the sample liquid into a diluent, and after evenly mixing, squeeze the air bag I (1) to take out a diluted liquid. The liquid moving device comprises two different measurement ranges, and can realize the measuring and moving of the sample liquid and the diluted liquid.

Description

Precise trace liquid dilution pipette and dilution pipetting method

Cross-reference to related applications

This application claims priority from a Chinese patent application filed on June 19, 2018 with the application number 201810629413.4, entitled "A Precision Micro Liquid Dilution Pipette", filed with the China Patent Office, the entire contents of which are incorporated herein by reference. in.

Technical field

The present application relates to the technical field of pipettes, and in particular, to a precise micro liquid dilution pipette and a dilution pipetting method.

Background technique

Micropipettes have been widely used in physical, chemical, and biological experiments. When performing physical and chemical operations, it is often necessary to dilute samples and then pipette. This operation requires two pipettes. In the prior art, In order to ensure the accuracy of the liquid removal, the operator needs to use two pipettes, which increases the experimental cost.

Summary of the Invention

The purpose of the present application includes providing a precise micro liquid dilution pipette and a dilution pipetting method, which can accurately take a liquid sample and can pipette the diluted dilution liquid.

The precise micro liquid dilution pipette provided by the present application comprises a balloon Ⅰ, a capillary Ⅰ, a capillary Ⅱ, and a sleeve, one end of the capillary Ⅱ is inserted into the balloon Ⅰ, and the other end of the capillary Ⅱ is inserted into the sleeve. The sleeve is provided with an inner recessed portion, the upper portion of which is connected to the lower port of capillary tube II, and the lower portion of the inner recessed portion is connected to the upper port of capillary tube I.

When the liquid needs to be aspirated, the lower port of the capillary Ⅰ is placed under the sample liquid surface, and the capillary Ⅰ is automatically filled with the sample liquid by capillary action. Due to the gap between the capillary Ⅰ and the capillary Ⅱ (the inner recess of the sleeve), the liquid cannot enter In capillary tube II, the lower port of capillary tube I is placed in the diluent. Squeeze the balloon I, and the liquid sucked out of capillary tube I will be discharged; the sample and the diluent are mixed to obtain the dilute solution of the rated concentration.

Squeeze the balloon Ⅰ again, and then place the lower port of the capillary Ⅰ under the liquid level of the diluent. The diluent enters the capillary Ⅰ, the inner recess of the sleeve, and the capillary Ⅱ in sequence until the capillary Ⅱ is filled with liquid, and the liquid flows from the capillary Ⅱ The upper port of the liquid overflows into the balloon Ⅰ. At this time, the total volume of the liquid in the capillary Ⅰ, the inner recess of the sleeve and the capillary Ⅱ is the total volume of the diluent that needs to be drawn.

Further, the airbag I is provided with a connection port, and the upper end of the capillary tube II is in sealed communication with the connection port, and the connection port is upwardly protruded and extends into the interior of the airbag I.

Further, the connection port is provided at the bottom of the airbag I, and the length of the capillary tube II extending into the inside of the airbag I is not less than 1/2 of the height of the airbag I.

Further, it further comprises an airbag II, which is in communication with the airbag I. Because the upper end portion of the capillary tube I is placed in the balloon I, it is easy to break the capillary tube I when squeezing the balloon I. Therefore, the balloon II is added, and the balloon II communicates with the balloon I. Squeezing the balloon II can achieve the same effect as squeezing the balloon I.

Further, the airbag II communicates with the airbag I through a connecting tube. The communication method between the airbag II and the airbag I is not limited to the method disclosed in the present application, as long as the airbag II can be squeezed and squeezed to achieve the purpose of sucking and removing liquid.

Further, the connection between the connecting tube and the airbag I is located on the top of the airbag I.

Further, a portion of the sleeve below the inner concave portion is a plug-in portion, and a plug-in hole is provided in the plug-in portion along its length direction, and the plug-in hole communicates with the inner concave portion;

The upper end of the capillary tube I is inserted into the insertion hole, and a gap exists between a part of the outer wall of the capillary tube I and the inner wall of the insertion hole. Connected.

Further, a cross section of the insertion hole of the insertion portion is a square or an equilateral triangle.

Further, the length of the plug-in portion of the sleeve is not greater than 1/2 of the length of the capillary I.

Further, the inner diameter of the capillary I is 0.2 to 2 mm.

Further, the inner diameter of the capillary tube II is 0.2 to 2 mm.

Further, the length of the capillary I is 1 to 100 mm, and the length of the capillary II is 1 to 100 mm.

Further, the range of the capillary I is 1 to 10 μL.

Further, the range of the capillary II is 10 to 200 μL.

Further, the internal volume of the concave portion in the sleeve is 1 to 2 μL.

The present application also provides a method for diluting and pipetting, which is configured to use the above-mentioned precise micro liquid dilution pipette to perform a dilution treatment on a sample liquid, and perform a pipetting on a diluent obtained after diluting with a diluent. The dilution steps include:

S100 extends the lower end of the capillary Ⅰ in the precise micro liquid dilution pipette below the liquid level of the sample liquid, and the capillary Ⅰ is automatically filled with the sample liquid by capillary action;

S200: Move the precise micro liquid dilution pipette to the container containing the diluent, squeeze the balloon Ⅰ, and the sample solution in the capillary tube Ⅰ is injected into the container containing the diluent. The sample solution is diluted after dilution liquid;

Among them, the pipetting operation steps of the diluted diluent include:

S300 squeezes the balloon Ⅰ, extends the lower end of the capillary Ⅰ in the pipette with a precise trace of liquid into the liquid level of the diluent, releases the balloon Ⅰ, and the diluent passes from the capillary Ⅰ under the action of pressure difference. The lower end is filled up with the capillary Ⅰ, the inner recess and the capillary Ⅱ in order;

S400 moves the precise micro-liquid dilution pipette to the destination container, squeezes the balloon I, and the diluent in the capillary I, the inner concave portion and the capillary II is injected into the destination container.

The precise micro liquid dilution pipette and dilution pipetting method provided by the present application include at least the following beneficial effects:

The present application discloses a precise micro-liquid dilution pipette and dilution pipetting method. The capillary liquid of capillary Ⅰ is used to aspirate and sample the liquid, and then the sample liquid is poured into the diluent. The diluent is evenly mixed to obtain the diluent; Squeeze the airbag I, and fill the diluent in the capillary Ⅰ, the inner recess and capillary Ⅱ, and move the precise micro liquid dilution pipette to transfer the diluent. Therefore, the precise micro liquid dilution pipette in this application can have two different ranges. One pipette can complete the pipetting of the sample liquid and the diluent, which is convenient to use and has low experimental cost.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 is a first schematic structural diagram of a precision micro liquid dilution pipette provided by the present application.

FIG. 2 is a schematic diagram of the connection between the airbag I and the airbag II in the precise micro liquid dilution pipette provided by the present application.

FIG. 3 is a schematic structural diagram of a capillary tube II in a precision micro liquid dilution pipette provided by the present application.

FIG. 4 is a schematic structural diagram of a cannula in a precision micro liquid dilution pipette provided by the present application.

FIG. 5 is a schematic structural diagram of a capillary tube I in a precise micro liquid dilution pipette provided by the present application.

FIG. 6 is a schematic diagram of a second structure of the precise micro liquid dilution pipette provided by the present application.

Reference numerals: 1-balloon I; 2-capillary II; 3-cannula; 4-capillary I; 5-inner recess; 6-balloon II; 7-connecting tube.

detailed description

The application can be better understood according to the following embodiments. However, those skilled in the art can easily understand that the content described in the embodiments is only used to explain the application, and should not and should not limit the details in the claims. This application is described.

In FIGS. 1 to 6, 1 is a balloon I, 2 is a capillary tube II, 3 is a cannula, 4 is a capillary tube I, 5 is a recessed portion, 6 is a balloon II, and 7 is a connecting tube.

The present application discloses a sample diluent pipette, which includes a balloon I1, a capillary tube I4, a capillary tube II2, and a sleeve 3, one end of the capillary tube II2 is inserted into the balloon I1, and the other end of the capillary tube II2 is inserted into the sleeve 3, and the sleeve 3 The middle part is provided with an inner recessed part, the upper part of the inner recessed part 5 is connected to the lower port of the capillary tube II2, and the lower part of the inner recessed part 5 is connected to the upper port of the capillary tube I4.

The present application also provides a method for diluting and pipetting, which is configured to use the above-mentioned precise micro liquid dilution pipette to perform a dilution treatment on a sample liquid, and perform a pipetting on a diluent obtained after diluting with a diluent. The dilution operation steps include: S100 extends the lower end of capillary Ⅰ4 in the precision micro liquid dilution pipette below the liquid level of the sample liquid, and capillary Ⅰ 4 automatically fills the sample liquid by capillary action; S200 moves the precision micro liquid dilution pipette to the container At the container with the diluent, squeeze the balloon I1, the sample solution in the capillary I4 is injected into the container containing the diluent, and the sample solution is diluted to obtain the diluent;

Among them, the pipetting operation steps of the diluted diluent include: S300 squeezes the balloon Ⅰ1, extends the lower end of the capillary Ⅰ4 in the pipette of the precise trace liquid into the diluent liquid level, releases the balloon Ⅰ1, and the diluent is under pressure Fill the capillary tube I4, the inner concave portion 5 and the capillary tube II2 from the lower end of the capillary tube I4 upwards in sequence under the difference action; S400 moves the precise micro liquid dilution pipette to the destination container, and squeezes the airbag I1, the capillary tube I4, the inner concave portion 5 and The diluent in the capillary tube II2 is poured into the destination container.

It should be noted that the "purpose container" herein refers to a place where a diluent is required for testing or experiments.

Specifically, the range of the capillary I4 can be designed to be 1 to 10 μL, the range of the capillary II 2 can be designed to be 10 to 200 μL, and the volume of the inner concave portion 5 of the sleeve 3 can be designed to be 1 to 2 μL.

When the liquid needs to be sampled, the lower port of capillary Ⅰ4 is placed below the liquid level of the sample liquid. Capillary Ⅱ is automatically filled with sample liquid by capillary action. Due to the gap between capillary Ⅰ4 and capillary Ⅱ2 (inner recess 5 of sleeve 3) ), The sample liquid can not enter the capillary Ⅱ2, and then the lower port of the capillary Ⅰ4 is placed in the diluent, squeeze the balloon Ⅰ1, the liquid absorbed in the capillary Ⅰ4 will be discharged into the diluent, and the sample liquid and the diluent are mixed, You can get the dilute solution of rated concentration.

When pipetting the diluent, squeeze the airbag Ⅰ1, and then place the lower port of capillary Ⅰ4 under the liquid surface of the diluent. The diluent will enter capillary Ⅰ4 and cannula 3 in sequence from the lower port of capillary Ⅰ4 under the effect of pressure difference. In the inner recess 5 and the capillary II2, until the capillary II2 is filled with the diluent, the total volume of the diluent in the capillary I4, the inner recess 5 of the sleeve 3 and the capillary II2 is the total volume of the diluent that needs to be drawn.

Capillary I4 in the micropipette dilutes the sample liquid by capillary action and transfers the sample liquid. The range of the capillary I4 is the volume of the sample liquid to be measured; The inner concave part 5 of the sleeve 3 and the capillary Ⅱ 2 absorb and transfer the diluent through the pressure difference. The total volume of the capillary Ⅰ4, the inner concave part 5 of the sleeve 3 and the capillary Ⅱ 2 is the volume of the diluent to be measured. The precision micro liquid dilution pipette can have two different ranges. One pipette can complete the pipetting of the sample liquid and the diluent, which is convenient to use and has low experimental cost.

Specifically, a connection port may be provided on the airbag I1, and the upper end of the capillary tube II2 is in sealed communication with the connection port, and the connection port is protruded upward to protrude into the inside of the airbag I1. When squeezing the airbag I1 to absorb the diluent, when the airbag I1 is completely inflated and returned to its original state, the volume of the diluent sucked through the capillary I4 is larger than the total volume of the capillary I4, the inner recess 5 of the sleeve 3 and the capillary II2 range. The diluent can flow into the airbag I1 through the upper port of the capillary Ⅱ2. This part of the diluent is located at the bottom of the airbag I1 under the action of gravity. The upper end of the capillary Ⅱ2 protrudes from the connection port and extends into the interior of the airbag I1 to a certain height. Height of the diluent in Ⅰ1), when the diluent is injected into the destination container by squeezing the airbag I1, the diluent in the airbag I1 is still located in the airbag I1, only the capillary Ⅱ2, the concave portion 5 of the sleeve 3 and the capillary Ⅰ4 The diluted solution flows out under the pressure difference and is injected into the destination container to ensure the accuracy of the volume of the pipette for the volume of the diluted solution.

Specifically, the connection port is provided at the bottom of the airbag I1, and the length of the capillary tube II2 extending into the inside of the airbag I1 is not less than 1/2 of the height of the airbag I1. Here is a specific limitation on the length of the capillary Ⅱ2 extending into the balloon Ⅰ1 to ensure that the diluent entering the balloon Ⅰ1 will not flood the upper port of the capillary Ⅱ2 when the diluent is sucked, so as to ensure that the volume of the diluent injected into the destination container is a capillary. Ⅱ2, the total volume of the inner recess 5 of the sleeve 3 and the capillary Ⅰ4 range, which improves the accuracy of the volume of the diluent and the volume of the pipette. In addition to the above limitation, the length of the capillary tube II2 extending into the interior of the airbag I1 can also be adjusted according to actual needs, and is not limited to the above range.

This application can also be designed to include two airbags, which connect airbag II6 to airbag I1. Squeezing airbag II6 can achieve the same effect as squeezing airbag I1. When it is necessary to squeeze airbag I1, it can be replaced by squeezing airbag II6. On the basis of realizing the suction or discharge of the airbag I1, reducing the occurrence of crushing of the capillary Ⅱ2 which directly extends into the airbag Ⅰ1 by squeezing the airbag Ⅰ1, thereby prolonging its service life, and reducing the damage to the operator caused by the breakage of the capillary Ⅱ2 hurt.

Specifically, the airbag I1 and the airbag II6 communicate with each other through a connecting tube 7, and the connection between the connecting tube 7 and the airbag I1 is located on the top of the airbag I1. When diluting the diluent, the excess diluent enters the airbag I1, and the connection between the connecting tube 7 and the airbag I1 is located on the top of the airbag I1, which can effectively reduce or even avoid the liquid seal caused by the diluent entering the airbag I1. To reduce the occurrence of adverse effects such as excessive dilution due to the effect of air flow in the airbag II6 and measuring the accuracy of the dilution.

In the present application, the upper part of the capillary I4 and the lower part of the capillary II2 are both placed in the sleeve 3, and the use of the sleeve 3 can strengthen the capillary I4 and the capillary II2.

In this embodiment, a portion of the sleeve 3 below the inner recessed portion 5 is a plug-in portion, and a plug-in hole is provided in the plug-in portion along its length direction, and the plug-in hole communicates with the inner recessed portion 5; the upper end of the capillary tube I4 is plugged in There is a gap between the outer wall of the capillary hole I4 and the inner wall of the socket hole in the plug hole, and the upper port of the capillary I4 communicates with the outside through the gap. The upper part of the capillary I4 is inserted into the insertion hole of the plug-in part, and is connected with the sleeve 3, and the upper port of the capillary I4 can communicate with the outside through the gap between the outer wall of the capillary and the inner wall of the plug hole, that is, the capillary The upper port of I4 communicates with the external air to ensure the capillary action strength of capillary I4, so that the sample liquid can be filled with capillary I4 under the action of capillary, thereby improving the precision of the micro liquid dilution. The pipette samples the volume of sample liquid through capillary I4 as the measuring range. Accuracy.

Specifically, the cross section of the insertion hole of the insertion portion may be a square or an equilateral triangle. Here is the specific structure of the plug-in hole. A square is used as an example. The cross-section of the plug-in hole is square. The plug-in hole is a square hole. The outer diameter of the capillary I4 matches the diameter of the largest inscribed circle of the square. The upper part of the capillary tube I4 is inserted into the plug hole along the length direction of the plug hole. The four tangent points of the outer circle of the capillary tube I4 are in contact with the midpoints of the four sides of the square, so that the capillary tube I4 and the plug hole are realized. The plug connection, specifically, the capillary Ⅰ4 and the plug hole can be a transition fit or an interference fit; outside the tangent, there is a gap between the four corners of the square and the outer wall of the capillary Ⅰ4, and the gap is along the plug The length of the contact hole communicates with the external air. Correspondingly, the upper port of the capillary tube I4 communicates with the external air through this gap, thereby ensuring that the capillary function of the capillary tube I4 is performed normally during the aspiration.

The principle when the cross-section of the plug-in hole is an equilateral triangle is similar to the above-mentioned square, and is not repeated here; and the cross-sectional shape of the plug-in hole is not limited to a square or an equilateral triangle. Other shapes can achieve the capillary Ⅰ4 card It can be fixed tightly and the upper port of the capillary I4 can communicate with the outside air.

It should be noted here that the upper port of the capillary I4 communicates with the external air through the gap. When the diluent is sucked, the diluent is affected by the liquid tension between the upper port of the capillary I4 and the gap and will not go down through the gap. Flow to ensure the proper flow of liquid.

Specifically, in this embodiment, the length of the insertion portion of the sleeve 3 is not greater than 1/2 of the length of the capillary I4. Here is a specific limitation on the length of the plug-in portion to ensure that the length of the capillary I4 protruding beyond the plug-in portion is reduced, and when the sample liquid is sucked and sampled, the length of the portion of the capillary I4 protruding beyond the plug-in portion is too short, which makes it easy to insert the plug. The lower end of the connection part is also inserted below the liquid level of the sample liquid, which causes the capillary I4 to fail to absorb liquid through capillary action.

Specifically, in this embodiment, the inner diameter of the capillary I4 may be 0.2 to 2 mm; the inner diameter of the capillary II 2 may be 0.2 to 2 mm; the length of the capillary I4 may be 1 to 100 mm, preferably 5 to 25 mm; and the length of the capillary II 2 may be 1 ~ 100mm, preferably 10 ~ 100mm.

In use: the range of capillary I can be designed to be 5 μL, the range of capillary II is 19 μL, and the range of the inner recess is 1 μL, so that 5 μL of sample and 25 μL of dilution can be drawn.

Industrial applicability

The precise micro liquid dilution pipette and dilution pipetting method provided by the application include two different measuring ranges. One pipette can complete the pipetting of the sample liquid and the diluent, which is convenient to use and has low experimental cost.

Claims

A precise micro-liquid dilution pipette is characterized in that it comprises a balloon Ⅰ (1), a capillary Ⅰ (4), a capillary Ⅱ (2), and a sleeve (3), and one end of the capillary Ⅱ (2) is inserted into the balloon In Ⅰ (1), the other end of the capillary Ⅱ (2) is inserted into a sleeve (3), and the sleeve (3) is provided with an inner recessed portion (5), and an upper portion of the inner recessed portion (5) and The lower port of the capillary II (2) is connected, and the lower part of the inner recess is connected to the upper port of the capillary I (4).
The precise micro liquid dilution pipette according to claim 1, wherein a connection port is provided on the balloon I (1), and an upper end of the capillary tube II (2) is in sealed communication with the connection port, and The connection port projects upward and projects into the interior of the airbag I (1).
The precise micro liquid dilution pipette according to claim 2, characterized in that the connection port is provided at the bottom of the balloon I (1), and the capillary tube II (2) extends into the balloon I ( 1) The internal length is not less than 1/2 of the height of the airbag I (1).
The precise micro-liquid dilution pipette according to any one of claims 1-3, further comprising a balloon II (6), wherein the balloon II (6) is in communication with the balloon I (1).
The precise micro-liquid dilution pipette according to claim 4, wherein the airbag II (6) communicates with the airbag I (1) through a connecting tube (7).
The precise micro-liquid dilution pipette according to claim 5, characterized in that the connection between the connecting tube (7) and the balloon I (1) is located on the top of the balloon I (1).
The precise micro-liquid dilution pipette according to any one of claims 1-6, characterized in that a portion of the sleeve (3) that is located below the recessed portion (5) is a plug-in portion, so A plug-in hole is provided in the plug-in portion along its length direction, and the plug-in hole is in communication with the inner recess (5);

The upper end of the capillary Ⅰ (4) is inserted into the insertion hole, and a gap exists between a part of the outer wall of the capillary Ⅰ (4) and the inner wall of the insertion hole, and the capillary Ⅰ (4) The upper port of is in communication with the outside through the gap.
The precise micro liquid dilution pipette according to claim 7, wherein a cross section of the insertion hole of the insertion portion is a square or an equilateral triangle.
The precise micro liquid dilution pipette according to claim 7 or 8, characterized in that the length of the plug-in portion of the sleeve (3) is not greater than 1/2 of the length of the capillary I (4).
The precise micro liquid dilution pipette according to any one of claims 1 to 9, characterized in that the inner diameter of the capillary I (2) is 0.2 to 2 mm.
The precise micro liquid dilution pipette according to any one of claims 1 to 10, wherein the inner diameter of the capillary tube II (4) is 0.2 to 2 mm.
The precise micro liquid dilution pipette according to any one of claims 1 to 11, characterized in that the length of the capillary I (2) is 1 to 100 mm, and the length of the capillary II (4) is 1 ~ 100mm.
The precise micro liquid dilution pipette according to any one of claims 1 to 12, characterized in that the range of the capillary I (2) is 1 to 10 μL.
The precise micro liquid dilution pipette according to any one of claims 1 to 13, characterized in that the range of the capillary II (4) is 10 to 200 μL.
The precise micro liquid dilution pipette according to any one of claims 1 to 14, wherein the internal volume of the inner recess (5) in the sleeve (3) is 1 to 2 μL.
A method for diluting and pipetting, characterized in that it is configured to use the precise micro liquid dilution pipette according to any one of claims 1 to 15, to dilute a sample liquid, and to dilute the sample liquid obtained after dilution with a diluent. Dilute the liquid to perform pipetting. The dilution of the sample liquid includes the following steps:

S100 extends the lower end of the capillary Ⅰ (4) in the precise micro liquid dilution pipette below the liquid level of the sample liquid, and the capillary Ⅰ (4) automatically sucks up the sample liquid by capillary action;

S200 moves the precise micro-liquid dilution pipette to the container containing the diluent, squeezes the balloon Ⅰ (1), and the sample solution in the capillary Ⅰ (4) is injected into the container containing the diluent, Dilute the sample solution to obtain a diluent;

Among them, the pipetting operation steps of the diluted diluent include:

S300 squeezes the airbag Ⅰ (1), extends the lower end of the capillary Ⅰ (4) in the pipette to the diluent liquid level, and releases the airbag Ⅰ (1). The capillary Ⅰ (4), the inner recess (5), and the capillary Ⅱ (2) are sequentially filled up from the lower end of the capillary Ⅰ (4) in the order of difference;

S400 moves the precise micro liquid dilution pipette to the destination container, squeezes the balloon Ⅰ (1), the capillary Ⅰ (4), the inner recessed portion (5), and the capillary Ⅱ (2) The diluted solution is poured into the destination container.