CN111957364A - Double-tube single-quantitative blood sampling pipette and blood sampling and pipetting method thereof - Google Patents
Double-tube single-quantitative blood sampling pipette and blood sampling and pipetting method thereof Download PDFInfo
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- CN111957364A CN111957364A CN202010853151.7A CN202010853151A CN111957364A CN 111957364 A CN111957364 A CN 111957364A CN 202010853151 A CN202010853151 A CN 202010853151A CN 111957364 A CN111957364 A CN 111957364A
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- 238000000034 method Methods 0.000 title claims abstract description 19
- 238000010241 blood sampling Methods 0.000 title claims description 31
- 239000008280 blood Substances 0.000 claims abstract description 103
- 210000004369 blood Anatomy 0.000 claims abstract description 103
- 239000007788 liquid Substances 0.000 claims description 83
- 238000003825 pressing Methods 0.000 claims description 26
- 238000007599 discharging Methods 0.000 claims description 13
- 239000003085 diluting agent Substances 0.000 claims description 12
- 238000009423 ventilation Methods 0.000 claims description 9
- 230000009471 action Effects 0.000 claims description 6
- 239000011521 glass Substances 0.000 claims description 3
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 claims description 2
- 229960002897 heparin Drugs 0.000 claims description 2
- 229920000669 heparin Polymers 0.000 claims description 2
- 238000013022 venting Methods 0.000 claims 1
- 238000005273 aeration Methods 0.000 description 4
- 230000007547 defect Effects 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 230000002093 peripheral effect Effects 0.000 description 2
- 238000007865 diluting Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/0203—Burettes, i.e. for withdrawing and redistributing liquids through different conduits
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Abstract
The invention discloses a double-tube single-quantitative blood collection pipette and a blood collection and pipetting method thereof.
Description
Technical Field
The invention relates to a double-tube single-quantitative blood sampling pipette and a blood sampling and pipetting method thereof.
Background
At present, a quantitative pipette is a pipetting tool for sucking a certain amount of liquid and then transferring the liquid to another place, and a conventional quantitative pipette structure is shown in fig. 1, and includes an air bag 91, a liquid storage bag 92, and a quantitative pipette 93, wherein the air bag 91 is connected to an upper end of the liquid storage bag 92, an upper end of the quantitative pipette 93 is inserted from a lower end of the liquid storage bag 92 and extends into the air bag 91 and the liquid storage bag 92, and the peripheral outer side of the quantitative pipette 93 is kept to be hermetically connected with the peripheral inner side of the lower end of the liquid storage bag 92, so that the quantitative pipette with the existing structure is formed, by squeezing the bladder 91 and then releasing, such that liquid is drawn from the dosing pipette 93 to fill the entire dosing pipette 93, excess liquid will overflow the upper end of the dosing pipette 93 into the lower reservoir 92, then the air bag 91 is squeezed again to discharge the liquid in the quantitative pipette 93, thus forming the whole sucking and pipetting process; however, the liquid overflowing the liquid storage bag 92 cannot be discharged by the squeezing air bag 91, so that a part of liquid sample is wasted, and great cost consumption is caused for expensive samples, and in addition, some novice operators have excessive force for squeezing the air bag 91, so that the quantitative pipette 93, the liquid storage bag 92 and the air bag 91 are completely filled with the liquid, and thus, the air pressure is insufficient, so that the pipetting fails; the quantitative pipette structure can derive various deformation structures, for example, a chinese patent with publication number CN205126256U, in which a liquid storage bag is communicated with and arranged at one side of the upper end of the quantitative pipette, and backflow is prevented by an overflow pipe designed obliquely, so the same disadvantage exists in the patent, so it is necessary to develop a pipette capable of squeezing, discharging and collecting redundant liquid samples and preventing excessive suction caused by poor control of squeezing pressure by a novice operator, and in addition, a structure capable of integrating blood collection and liquid transfer is also the development focus of the present invention.
Disclosure of Invention
Aiming at the defects of the prior art, the invention solves the problems that: the double-tube single-quantitative blood sampling pipette and the blood sampling and pipetting method thereof have the advantages that the blood collection and pipetting are integrated, the quantitative pipetting can be realized, the redundant liquid samples can be squeezed, discharged and collected, and the defect of excessive suction caused by the fact that a novice operator cannot easily control the squeezing pressure is overcome.
In order to solve the problems, the technical scheme adopted by the invention is as follows:
a double-tube single-quantitative blood sampling pipette comprises an upper air bag, an air pipe, a lower air bag, a pipette and a micro blood sampling tube; the upper end of the upper air bag is closed, and the lower end of the upper air bag is provided with an upper opening; the lower air bag is positioned right below the upper air bag; the upper end of the lower air bag is closed, and the lower end of the lower air bag is provided with a lower opening; two air pipes are arranged; the two vent pipes are respectively arranged outside two sides of the lower air bag; the upper ends of the two breather pipes extend and meet to form an upper end extending and meeting part; the upper end extension intersection is positioned right above the lower air bag; the upper ends of the extending and meeting positions of the upper ends of the two vent pipes are communicated with the upper opening at the lower end of the upper air bag; the pipette is arranged right below the two vent pipes and the lower air bag; the lower ends of the two vent pipes extend and meet to form a lower end extending and meeting part; the lower end extension intersection is positioned right below the lower opening of the lower air bag; the upper ends of the lower end extension junctions of the two vent pipes are communicated with the lower opening of the lower air bag, and the lower ends of the lower end extension junctions of the two vent pipes are communicated with the upper end of the pipette; the micro blood collection tube is arranged below the pipette; the upper end of the micro blood collection tube is inserted into the lower end of the pipette, and the lower end of the micro blood collection tube extends to the lower part of the pipette; the upper end of the micro blood collection tube is communicated with the external air pressure of the pipette.
Further, a cavity with a square-shaped cross section is arranged in the pipette; the cross section of the outer side of the periphery of the micro blood collection tube is of a circular structure; the outer sides of the periphery of the upper end of the micro blood collection tube are connected with the inner walls of the periphery of the cavity of the pipette; a plurality of ventilation gaps are uniformly arranged between the outer sides of the periphery of the micro blood collection tube and the inner wall of the cavity of the pipette.
Furthermore, the periphery of the upper end of the micro blood collection tube is connected with the inner part of the lower end of the pipette in a sealing way; an air vent is formed in one side below the pipette; the air vent extends downwards to be connected with an air nozzle pipe; the lower end face of the air nozzle pipe is positioned above the lower end face of the micro blood collection pipe; the vent is located the top of trace heparin tube up end.
Furthermore, the upper end face of the pipette is connected to the outer side face of the lower end extending intersection of the two vent pipes.
Further, the two vent pipes are respectively connected to the two sides of the lower air bag through plastic pieces in a curing manner.
Further, the pipette is a glass tube.
Further, the pipette is a plastic tube.
Furthermore, a ventilating and water-tight filter element is arranged at the lower opening of the lower air bag; the upper end and the lower end of the ventilation water-blocking filter element respectively extend into the extension intersection of the inner part and the lower end of the lower air bag.
A blood sampling and pipetting method of a double-tube single-quantitative blood sampling pipette comprises the following steps:
s1, blood sampling: contacting the lower end of the micro blood collection tube below the pipette with blood to be collected, sucking the blood through the capillary action of the micro blood collection tube until the whole micro blood collection tube is filled, and then pressing the upper air bag or the lower air bag to squeeze the blood in the micro blood collection tube into a diluent;
s2, pressing of the lower air bag: firstly, pressing the lower air bag, then contacting the lower end of the pipette with the diluent to be sucked, loosening the lower air bag, sucking the liquid from the lower end of the pipette and filling the whole pipette, and sucking the liquid to the extension intersection of the lower ends of the two vent pipes and the inside of the lower air bag;
s3, pressing of the upper air bag: pressing the upper air bag, extruding and discharging liquid in the whole pipette and liquid at the lower half part of the extending intersection of the lower ends of the two vent pipes by air pressure in the upper air bag, and realizing pipetting;
s4, discharging and collecting residual liquid: the lower air bag is pressed again, so that the liquid in the lower air bag and in the upper half part of the extension intersection of the lower ends of the two vent pipes is discharged from the lower end of the pipette and collected.
A blood sampling and pipetting method of a double-tube single-quantitative blood sampling pipette comprises the following steps:
s1, blood sampling: contacting the lower end of the micro blood collection tube below the pipette with blood to be collected, sucking the blood through the capillary action of the micro blood collection tube until the whole micro blood collection tube is filled, and then pressing the upper air bag or the lower air bag to squeeze the blood in the micro blood collection tube into a diluent;
s2, pressing of the upper air bag: firstly, pressing the upper air bag, then contacting the lower end of the pipette with the diluent to be sucked, loosening the upper air bag, sucking the liquid from the lower end of the pipette and filling the whole pipette, and sucking the liquid to the extending intersection of the lower ends of the two vent pipes and the interiors of the two vent pipes;
s3, pressing of the lower air bag: the lower air bag is pressed, and liquid in the whole pipette and liquid at the extending and meeting part of the lower ends of the two vent pipes are extruded and discharged through air pressure in the lower air bag, so that the aim of pipetting is fulfilled;
s4, discharging and collecting residual liquid: the upper balloon is again squeezed so that the liquid in both air ducts is drained from the lower end of the pipette and collected.
The invention has the advantages of
1. According to the invention, the micro blood collection tube is arranged below the pipette, and blood is collected by the capillary principle of the micro blood collection tube, so that the function of collecting blood is realized.
2. The invention sucks and discharges liquid and cooperates and uses through two gasbags of upper gasbag and lower gasbag, seal between upper end of the lower gasbag and the lower gasbag, extend the aeration route of the upper gasbag to the lower part of the lower gasbag through the breather pipe of both sides of lower end, and the pipette is communicated and designed under the lower gasbag and breather pipe, so the route that two gasbags suck liquid does not interfere with each other, can do one gasbag suck liquid, another gasbag carries on the discharge of the liquid quantitatively, extrude the surplus liquid sample and reclaim it through the gasbag that originally sucks the liquid after discharging the liquid, thus the invention can not only transfer liquid quantitatively but also reclaim the purpose of the surplus liquid sample, in addition because of the independent aeration route of two gasbags, no matter which gasbag takes place the phenomenon of the transition suction when sucking, the other air bag can discharge the quantitative liquid, and the liquid sample pipetting operation is not influenced.
Drawings
Fig. 1 is a schematic structural diagram of the prior art.
Fig. 2 is a schematic structural diagram of the present invention.
FIG. 3 is a schematic front view showing the structure of a micro blood collection tube and a pipette in embodiment 1 of the present invention.
FIG. 4 is a schematic sectional view showing the structure of a micro blood collection tube and a pipette in embodiment 1 of the present invention.
FIG. 5 is a schematic front view showing the structure of a micro blood collection tube and a pipette in embodiment 2 of the present invention.
Fig. 6 is a schematic structural diagram of embodiment 3 of the present invention.
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings.
Example 1
As shown in fig. 2 to 4, a double-tube single-quantitative blood collection pipette includes an upper air bag 1, a vent tube 3, a lower air bag 2, a pipette 4, and a micro blood collection tube 5; the upper end of the upper air bag 1 is closed, and the lower end of the upper air bag 1 is provided with an upper opening 11; the lower air bag 2 is positioned right below the upper air bag 1; the upper end of the lower air bag 2 is closed, and the lower end of the lower air bag 2 is provided with a lower opening 21; two air pipes 3 are arranged; the two vent pipes 3 are respectively arranged outside the two sides of the lower air bag 2; the upper ends of the two air pipes 3 extend and meet to form an upper end extending and meeting part 31; the upper end extension intersection 31 is located directly above the lower air bag 1; the upper ends of the extending and meeting parts 31 of the upper ends of the two vent pipes 3 are communicated with the upper opening 11 at the lower end of the upper air bag 1; the pipette 4 is arranged right below the two vent pipes 3 and the lower air bag 2; the lower ends of the two air pipes 3 extend and meet to form a lower end extending and meeting part 32; the lower end extension intersection 32 is located directly below the lower opening 21 of the lower bag 2; the upper ends of the lower end extension junctions 32 of the two vent pipes 3 are communicated with the lower opening 21 of the lower air bag 2, and the lower ends of the lower end extension junctions 32 of the two vent pipes 3 are communicated with the upper end of the pipette 4; the micro blood collection tube 5 is arranged below the pipette 4; the upper end of the micro blood collection tube 5 is inserted into the lower end of the pipette 4, and the lower end of the micro blood collection tube 5 extends to the lower part of the pipette 4; the upper end of the micro blood collection tube 5 is communicated with the external air pressure of the pipette 4. Further preferably, a cavity with a square-shaped cross section is arranged inside the pipette 4; the cross section of the outer side of the periphery of the micro blood collection tube 5 is of a circular structure; the outer side of the periphery of the upper end of the micro blood collection tube 5 is connected with the inner wall of the periphery of the cavity of the pipette 4; evenly be equipped with a plurality of ventilation gap 41 between the outside all around of micro blood collection tube 5 and the cavity inner wall of pipette 4, so this embodiment is ventilated with the outside through a plurality of ventilation gap 41 between the outside all around of micro blood collection tube 5 and the cavity inner wall of pipette 4 to micro blood collection tube 5 utilizes the capillary principle to take a blood sample. Further preferably, the upper end surface of the pipette 4 is connected to the outer lower end surface of the lower end extension junction 32 of the two air tubes 3. Further, the two vent pipes 3 are respectively connected to the two sides of the lower air bag 2 through plastic parts in a curing manner. Further, the pipette 4 is a glass tube or a plastic tube. The interior of the pipette is generally a circular cavity structure, and the pipette capacity is determined by its inner diameter and length.
Example 2
This example is substantially the same as example 1, except that the upper end of the micro blood collection tube 5 is configured to be externally ventilated with the pipette 4, and as shown in fig. 5, the periphery of the upper end of the micro blood collection tube 5 is hermetically connected with the inside of the lower end of the pipette 4; an air vent 41 is formed at one side below the pipette 4; the air port 41 is connected with an air nozzle pipe 42 in a downward extending way; the lower end surface of the air nozzle tube 42 is positioned above the lower end surface of the micro blood collection tube 5; the vent 41 is located above the upper end surface of the micro blood collection tube 5. In this embodiment, the upper end of the micro blood collection tube 5 is ventilated to the outside through the vent hole 41 on the pipette 4 side.
Examples 1 and 2 of the present invention have the following two blood sampling and pipetting methods for the upper bag 1 and the lower bag 2.
The first method comprises the following steps: as shown in fig. 2 to 5, a pipetting method of a double-tube single-quantitative blood sampling pipette comprises the following steps: s1, blood sampling: contacting the lower end of the micro blood collection tube 5 below the pipette 4 with blood to be collected, sucking the blood through the capillary action of the micro blood collection tube 5 until the whole micro blood collection tube 5 is filled, and then squeezing the blood in the micro blood collection tube 5 into a diluent by pressing the upper air bag 1 or the lower air bag 2; s2, pressing of the lower air bag: firstly, the lower air bag 2 is pressed, then the lower end of the pipette 4 is contacted with the diluent to be sucked, the lower air bag 2 is loosened, the liquid is sucked from the lower end of the pipette 4 and fills the whole pipette 4, and the liquid is sucked to the lower end extending junction 32 of the two air pipes 3 and the inside of the lower air bag 2; s3, pressing of the upper air bag: pressing the upper air bag 1, extruding and discharging the liquid in the whole pipetting tube 4 and the liquid in the lower half part of the extending intersection 32 of the lower ends of the two vent tubes 3 through the air pressure in the upper air bag 1, and realizing pipetting; s4, discharging and collecting residual liquid: the lower balloon 2 is again squeezed so that the liquid inside the lower balloon 2 and in the upper half of the lower extending intersection 32 of the two vent tubes 3 is drained from the lower end of the pipette 4 and collected.
And the second method comprises the following steps: as shown in fig. 2 to 5, a pipetting method of a double-tube single-quantitative blood sampling pipette comprises the following steps: s1, blood sampling: contacting the lower end of a micro blood collection tube 5 below a pipette 4 with blood to be collected, sucking the blood through the capillary action of the micro blood collection tube 5 until the whole micro blood collection tube 5 is filled, and then pressing an upper air bag 1 or a lower air bag 2 to extrude the blood in the micro blood collection tube into a diluent; s2, pressing of the upper air bag: firstly, pressing the upper air bag 1, then contacting the lower end of the pipette 4 with the diluent to be sucked, loosening the upper air bag 1, sucking the liquid from the lower end of the pipette 4 and filling the whole pipette, and sucking the liquid to the extending junction 32 of the lower ends of the two vent pipes 3 and the insides of the two vent pipes 3; s3, pressing of the lower air bag: the lower air bag 2 is pressed, and the liquid in the whole pipette 4 and the liquid at the lower end extension intersection 32 of the two air pipes 3 are extruded and discharged through the air pressure in the lower air bag 2, so that the aim of pipetting is fulfilled; s4, discharging and collecting residual liquid: the upper air bag 1 is pressed again so that the liquid in the two air tubes 3 is discharged from the lower end of the pipette 4 and collected.
Example 3
The present embodiment is different from embodiment 1 or embodiment 2 in that a filter element 8 for ventilation and water non-passage is provided at a lower opening 21 of the lower air bag 2. As shown in fig. 6, the upper and lower ends of the air-permeable and water-impermeable filter element 8 extend into the interior of the lower air bag 2 and the lower end extension junction 32, respectively, so that the blood sampling and pipetting method of the present invention may be preferably as follows: after the blood sampling and diluting step, the lower air bag 2 is firstly extruded, after the liquid is sucked by the pipette 4, the path for the liquid to enter is automatically closed when the liquid reaches the lower side of the ventilating and water-tight filter element 8, the pipette 4 automatically stops sucking the liquid, and then the upper air bag 1 is extruded, and the pipette 4 and the liquid in the lower end extension intersection 32 are all discharged, so that the liquid transfer is realized, the subsequent process of discharging and recycling the residual liquid is not needed, and the use is simpler and more convenient; of course, the upper bag 1 may be preferentially pressed by the method according to the second embodiment of example 1 or example 2.
According to the invention, the micro blood collection tube 5 is arranged below the pipette 4, and the function of collecting blood is realized by collecting blood according to the capillary principle of the micro blood collection tube 5, the ventilation position at the upper end of the micro blood collection tube 5 is designed between the outer side of the periphery of the micro blood collection tube 5 and the inner wall of the cavity of the pipette 4, or the ventilation position at the upper end of the micro blood collection tube 5 is designed on the side wall of one side of the pipette 4, so that a through hole does not need to be formed in the micro blood collection tube 5, and the defects of poor finished product rate and high process difficulty in forming the through hole in the micro blood collection tube 5 are avoided.
The invention sucks and discharges liquid and cooperates and uses through two gasbags of upper gasbag 1 and lower gasbag 2, seal between upper end of the gasbag 2 of the lower part and the lower end of the upper gasbag 1, extend the aeration route of the upper gasbag 1 to the lower part of the gasbag 2 of the lower part through the breather pipe 3 of both sides of lower end, and the pipette 4 is communicated and designed under the gasbag 2 of the lower part and breather pipe 3, so the route that two gasbags suck liquid does not interfere each other, can do a gasbag suck liquid, another gasbag carries on the quantitative liquid discharge, extrude the surplus liquid sample and reclaim and avoid wasting through the gasbag that sucks liquid originally after discharging liquid, so the invention can not only realize the goal of reclaiming the surplus liquid sample quantitatively pipetting, in addition because of the independent aeration route of two gasbags, no matter which one gasbag takes place the phenomenon of transdraughting when sucking, the other air bag can discharge the quantitative liquid, and the liquid sample pipetting operation is not influenced.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (9)
1. A double-tube single-quantitative blood sampling pipette is characterized by comprising an upper air bag, a vent pipe, a lower air bag, a pipette and a micro blood sampling tube; the upper end of the upper air bag is closed, and the lower end of the upper air bag is provided with an upper opening; the lower air bag is positioned right below the upper air bag; the upper end of the lower air bag is closed, and the lower end of the lower air bag is provided with a lower opening; two air pipes are arranged; the two vent pipes are respectively arranged outside two sides of the lower air bag; the upper ends of the two breather pipes extend and meet to form an upper end extending and meeting part; the upper end extension intersection is positioned right above the lower air bag; the upper ends of the extending and meeting positions of the upper ends of the two vent pipes are communicated with the upper opening at the lower end of the upper air bag; the pipette is arranged right below the two vent pipes and the lower air bag; the lower ends of the two vent pipes extend and meet to form a lower end extending and meeting part; the lower end extension intersection is positioned right below the lower opening of the lower air bag; the upper ends of the lower end extension junctions of the two vent pipes are communicated with the lower opening of the lower air bag, and the lower ends of the lower end extension junctions of the two vent pipes are communicated with the upper end of the pipette; the micro blood collection tube is arranged below the pipette; the upper end of the micro blood collection tube is inserted into the lower end of the pipette, and the lower end of the micro blood collection tube extends to the lower part of the pipette; the upper end of the micro blood collection tube is communicated with the external air pressure of the pipette.
2. The dual-barreled single-dose blood collection pipette of claim 1, wherein the pipette is internally provided with a cavity having a square-shaped cross-section; the cross section of the outer side of the periphery of the micro blood collection tube is of a circular structure; the outer sides of the periphery of the upper end of the micro blood collection tube are connected with the inner walls of the periphery of the cavity of the pipette; a plurality of ventilation gaps are uniformly arranged between the outer sides of the periphery of the micro blood collection tube and the inner wall of the cavity of the pipette.
3. The double-barreled single-dose blood collection pipette according to claim 1, wherein the periphery of the upper end of the micro blood collection tube is hermetically connected with the inside of the lower end of the pipette; an air vent is formed in one side below the pipette; the air vent extends downwards to be connected with an air nozzle pipe; the lower end face of the air nozzle pipe is positioned above the lower end face of the micro blood collection pipe; the vent is located the top of trace heparin tube up end.
4. The dual-barreled single unit dose blood collection pipette of claim 1, wherein the upper end face of the pipette is connected to the outer side face of the lower end of the two vent tubes where the lower ends extend to meet.
5. The dual-barreled single dosing blood collection pipette of claim 1, wherein the two vent tubes are respectively attached to the exterior of both sides of the lower balloon by a plastic part cured.
6. The dual-barreled single serve blood collection pipette of claim 1, wherein the pipette is a glass or plastic tube.
7. The dual-barreled single-dose blood collection pipette according to claim 1, wherein a venting and water-tight filter element is provided at a lower opening of the lower air bag; the upper end and the lower end of the ventilation water-blocking filter element respectively extend into the extension intersection of the inner part and the lower end of the lower air bag.
8. A blood sampling pipetting method with a double tube single dosing blood sampling pipette according to claim 1, characterised by the following steps:
s1, blood sampling: contacting the lower end of the micro blood collection tube below the pipette with blood to be collected, sucking the blood through the capillary action of the micro blood collection tube until the whole micro blood collection tube is filled, and then pressing the upper air bag or the lower air bag to squeeze the blood in the micro blood collection tube into a diluent;
s2, pressing of the lower air bag: firstly, pressing the lower air bag, then contacting the lower end of the pipette with the diluent to be sucked, loosening the lower air bag, sucking the liquid from the lower end of the pipette and filling the whole pipette, and sucking the liquid to the extension intersection of the lower ends of the two vent pipes and the inside of the lower air bag;
s3, pressing of the upper air bag: pressing the upper air bag, extruding and discharging liquid in the whole pipette and liquid at the lower half part of the extending intersection of the lower ends of the two vent pipes by air pressure in the upper air bag, and realizing pipetting;
s4, discharging and collecting residual liquid: the lower air bag is pressed again, so that the liquid in the lower air bag and in the upper half part of the extension intersection of the lower ends of the two vent pipes is discharged from the lower end of the pipette and collected.
9. A blood sampling pipetting method with a double tube single dosing blood sampling pipette according to claim 1, characterised by the following steps:
s1, blood sampling: contacting the lower end of the micro blood collection tube below the pipette with blood to be collected, sucking the blood through the capillary action of the micro blood collection tube until the whole micro blood collection tube is filled, and then pressing the upper air bag or the lower air bag to squeeze the blood in the micro blood collection tube into a diluent;
s2, pressing of the upper air bag: firstly, pressing the upper air bag, then contacting the lower end of the pipette with the diluent to be sucked, loosening the upper air bag, sucking the liquid from the lower end of the pipette and filling the whole pipette, and sucking the liquid to the extending intersection of the lower ends of the two vent pipes and the interiors of the two vent pipes;
s3, pressing of the lower air bag: the lower air bag is pressed, and liquid in the whole pipette and liquid at the extending and meeting part of the lower ends of the two vent pipes are extruded and discharged through air pressure in the lower air bag, so that the aim of pipetting is fulfilled;
s4, discharging and collecting residual liquid: the upper balloon is again squeezed so that the liquid in both air ducts is drained from the lower end of the pipette and collected.
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| CN202010853151.7A CN111957364B (en) | 2020-08-22 | 2020-08-22 | Double-tube single-quantitative blood sampling pipette and blood sampling pipetting method thereof |
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