CN111819153A - 利用微流控芯片构型和动力学进行光力测量和细胞成像的微流控芯片设备 - Google Patents

利用微流控芯片构型和动力学进行光力测量和细胞成像的微流控芯片设备 Download PDF

Info

Publication number
CN111819153A
CN111819153A CN201780098314.1A CN201780098314A CN111819153A CN 111819153 A CN111819153 A CN 111819153A CN 201780098314 A CN201780098314 A CN 201780098314A CN 111819153 A CN111819153 A CN 111819153A
Authority
CN
China
Prior art keywords
channel
substrate
chip
cell
particles
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201780098314.1A
Other languages
English (en)
Chinese (zh)
Inventor
肖恩·哈特
科林·赫伯特
克里斯多夫·菲尔德
施薇塔·克里斯南
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Lumaset Co ltd
Lumacyte LLC
Original Assignee
Lumaset Co ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lumaset Co ltd filed Critical Lumaset Co ltd
Publication of CN111819153A publication Critical patent/CN111819153A/zh
Pending legal-status Critical Current

Links

Images

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B81—MICROSTRUCTURAL TECHNOLOGY
    • B81B—MICROSTRUCTURAL DEVICES OR SYSTEMS, e.g. MICROMECHANICAL DEVICES
    • B81B1/00—Devices without movable or flexible elements, e.g. microcapillary devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N15/1434—Electro-optical investigation, e.g. flow cytometers using an analyser being characterised by its optical arrangement
    • G01N15/1436—Electro-optical investigation, e.g. flow cytometers using an analyser being characterised by its optical arrangement the optical arrangement forming an integrated apparatus with the sample container, e.g. a flow cell
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N15/1484—Electro-optical investigation, e.g. flow cytometers microstructural devices
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N21/05—Flow-through cuvettes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/08—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0663—Stretching or orienting elongated molecules or particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0668—Trapping microscopic beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0874—Three dimensional network
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0454—Moving fluids with specific forces or mechanical means specific forces radiation pressure, optical tweezers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B01L2400/049—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics vacuum
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N15/1468—Electro-optical investigation, e.g. flow cytometers with spatial resolution of the texture or inner structure of the particle
    • G01N15/147—Electro-optical investigation, e.g. flow cytometers with spatial resolution of the texture or inner structure of the particle the analysis being performed on a sample stream
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1006—Investigating individual particles for cytology
CN201780098314.1A 2017-12-23 2017-12-23 利用微流控芯片构型和动力学进行光力测量和细胞成像的微流控芯片设备 Pending CN111819153A (zh)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/US2017/068373 WO2019125502A1 (en) 2017-12-23 2017-12-23 Microfluidic chip device for optical force measurements and cell imaging using microfluidic chip configuration and dynamics

Publications (1)

Publication Number Publication Date
CN111819153A true CN111819153A (zh) 2020-10-23

Family

ID=66995019

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201780098314.1A Pending CN111819153A (zh) 2017-12-23 2017-12-23 利用微流控芯片构型和动力学进行光力测量和细胞成像的微流控芯片设备

Country Status (12)

Country Link
EP (1) EP3728107A4 (ru)
JP (2) JP7390305B2 (ru)
KR (2) KR102602599B1 (ru)
CN (1) CN111819153A (ru)
AU (2) AU2017443695B2 (ru)
BR (1) BR112020012748A2 (ru)
CA (1) CA3086498A1 (ru)
GB (1) GB2584218A (ru)
MX (1) MX2020007182A (ru)
RU (1) RU2764676C1 (ru)
SG (1) SG11202005872XA (ru)
WO (1) WO2019125502A1 (ru)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114088588A (zh) * 2021-10-27 2022-02-25 西安理工大学 一种基于无透镜成像的红细胞三维尺寸测量方法

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102656008B1 (ko) * 2020-09-08 2024-04-11 경북대학교 산학협력단 질병 진단용 키트, 질병 진단용 키트를 이용한 질병 진단 방법 및 질병 진단용 키트의 제조 방법
CN112986063B (zh) * 2021-01-18 2022-04-15 西北大学 高通量染色体和细胞骨架应变流式分析仪及实施方法

Citations (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5768545U (ru) * 1980-10-15 1982-04-24
US5184192A (en) * 1991-07-17 1993-02-02 Millipore Corporation Photometric apparatus with a flow cell coated with an amorphous fluoropolymer
US20020181837A1 (en) * 2000-11-28 2002-12-05 Mark Wang Optical switching and sorting of biological samples and microparticles transported in a micro-fluidic device, including integrated bio-chip devices
JP2004069431A (ja) * 2002-08-05 2004-03-04 Plant Technos:Kk 液中粒子の画像解析装置
JP2004130219A (ja) * 2002-10-10 2004-04-30 Kawamura Inst Of Chem Res マイクロ流体素子、流体処理デバイス、および流体処理方法
US20050002025A1 (en) * 2003-05-15 2005-01-06 Toshiba Ceramics Co., Ltd. Channel structure and process for production thereof
JP2007148981A (ja) * 2005-11-30 2007-06-14 Univ Waseda 微粒子分別マイクロシステムおよび微粒子分別方法
US20080067128A1 (en) * 2005-03-11 2008-03-20 Centre National De La Recherche Scientifique Fluid separation device
JP2008191119A (ja) * 2007-02-08 2008-08-21 Citizen Holdings Co Ltd 流体試料用フローセル
US20090032449A1 (en) * 2002-07-31 2009-02-05 Arryx, Inc. Multiple laminar flow-based particle and cellular separation with laser steering
US20090140170A1 (en) * 2005-08-11 2009-06-04 Eksigent Technologies, Llc Microfluidic systems, devices and methods for reducing background autofluorescence and the effects thereof
US20130264295A1 (en) * 2012-04-05 2013-10-10 Samsung Electronics Co., Ltd. Filter for capturing target material
CN103926190A (zh) * 2014-05-08 2014-07-16 齐鲁工业大学 基于微流控系统的单细胞自动分析方法
US20140220557A1 (en) * 2007-12-21 2014-08-07 Sean J. Hart Device and Method for Laser Analysis and Separation (LAS) of Particles
US20150316188A1 (en) * 2012-06-29 2015-11-05 University Of South Australia Fluid connection ports
CN106255888A (zh) * 2014-04-25 2016-12-21 伯克利照明有限公司 在同一微流体装置的不同部分中的dep力控制和电润湿控制
JP2016217888A (ja) * 2015-05-20 2016-12-22 シスメックス株式会社 細胞検出装置および細胞検出方法
US20160369337A1 (en) * 2007-02-05 2016-12-22 Intelligent Biosystems, Inc. Detection device and methods of use
US20170227442A1 (en) * 2007-12-21 2017-08-10 Government Of The United States Of America, As Represented By The Secretary Of The Navy Device for laser analysis and separation (las) of particles

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS63241337A (ja) * 1986-11-07 1988-10-06 Hitachi Ltd 光度計用フローセル
US5214593A (en) * 1990-11-07 1993-05-25 Rainin Instrument Co., Inc. Method and apparatus for extending the linear dynamic range of absorbance detectors including multi-lightpath flow cells
US6167910B1 (en) * 1998-01-20 2001-01-02 Caliper Technologies Corp. Multi-layer microfluidic devices
CN2662247Y (zh) * 2003-04-25 2004-12-08 谭玉山 基于白光反射干涉频谱变化规律的光纤阵列生物芯片
EP1889034A1 (en) * 2005-05-24 2008-02-20 Agilent Technologies, Inc. Multi-path flow cell correction
JP4064445B1 (ja) * 2007-10-26 2008-03-19 リオン株式会社 粒子測定装置
RU2380418C1 (ru) * 2008-10-01 2010-01-27 Учреждение Российской академии наук Институт молекулярной биологии им. В.А. Энгельгардта РАН Сменный микрофлюидный модуль для автоматизированного выделения и очистки нуклеиновых кислот из биологических образцов и способ выделения и очистки нуклеиновых кислот с его использованием
JP5901121B2 (ja) * 2011-02-16 2016-04-06 キヤノン株式会社 流路デバイス、及び該流路デバイスを用いた液体の搬送方法
RU2510509C1 (ru) * 2012-07-16 2014-03-27 Федеральное государственное бюджетное учреждение науки Институт цитологии и генетики Сибирского отделения Российской академии наук Микрофлюидная система для проведения иммуноанализа
US20140193892A1 (en) * 2012-07-25 2014-07-10 Theranos, Inc. Image analysis and measurement of biological samples
JP2014032098A (ja) * 2012-08-03 2014-02-20 Hitachi High-Technologies Corp 液体クロマトグラフ分析装置
US9529203B2 (en) * 2012-09-17 2016-12-27 Cytonome/St, Llc Focal plane shifting system
US9731293B2 (en) * 2012-10-03 2017-08-15 The United States Of America, As Represented By The Secretary Of The Navy Paired laser and electrokinetic separation, manipulation, and analysis device
JP6332098B2 (ja) * 2015-03-25 2018-05-30 株式会社島津製作所 フローセル

Patent Citations (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5768545U (ru) * 1980-10-15 1982-04-24
US5184192A (en) * 1991-07-17 1993-02-02 Millipore Corporation Photometric apparatus with a flow cell coated with an amorphous fluoropolymer
JPH05196565A (ja) * 1991-07-17 1993-08-06 Millipore Corp 測光装置
US20020181837A1 (en) * 2000-11-28 2002-12-05 Mark Wang Optical switching and sorting of biological samples and microparticles transported in a micro-fluidic device, including integrated bio-chip devices
US20090032449A1 (en) * 2002-07-31 2009-02-05 Arryx, Inc. Multiple laminar flow-based particle and cellular separation with laser steering
JP2004069431A (ja) * 2002-08-05 2004-03-04 Plant Technos:Kk 液中粒子の画像解析装置
JP2004130219A (ja) * 2002-10-10 2004-04-30 Kawamura Inst Of Chem Res マイクロ流体素子、流体処理デバイス、および流体処理方法
US20050002025A1 (en) * 2003-05-15 2005-01-06 Toshiba Ceramics Co., Ltd. Channel structure and process for production thereof
US20080067128A1 (en) * 2005-03-11 2008-03-20 Centre National De La Recherche Scientifique Fluid separation device
JP2008533460A (ja) * 2005-03-11 2008-08-21 セントル ナショナル ドゥ ラ ルシェルシュ シアンティフィック 流体分離装置
US20090140170A1 (en) * 2005-08-11 2009-06-04 Eksigent Technologies, Llc Microfluidic systems, devices and methods for reducing background autofluorescence and the effects thereof
JP2007148981A (ja) * 2005-11-30 2007-06-14 Univ Waseda 微粒子分別マイクロシステムおよび微粒子分別方法
US20160369337A1 (en) * 2007-02-05 2016-12-22 Intelligent Biosystems, Inc. Detection device and methods of use
JP2008191119A (ja) * 2007-02-08 2008-08-21 Citizen Holdings Co Ltd 流体試料用フローセル
US20140220557A1 (en) * 2007-12-21 2014-08-07 Sean J. Hart Device and Method for Laser Analysis and Separation (LAS) of Particles
US20170227442A1 (en) * 2007-12-21 2017-08-10 Government Of The United States Of America, As Represented By The Secretary Of The Navy Device for laser analysis and separation (las) of particles
US20130264295A1 (en) * 2012-04-05 2013-10-10 Samsung Electronics Co., Ltd. Filter for capturing target material
US20150316188A1 (en) * 2012-06-29 2015-11-05 University Of South Australia Fluid connection ports
CN106255888A (zh) * 2014-04-25 2016-12-21 伯克利照明有限公司 在同一微流体装置的不同部分中的dep力控制和电润湿控制
KR20160146975A (ko) * 2014-04-25 2016-12-21 버클리 라잇츠, 인크. 동일한 미세유체 장치의 상이한 섹션들에서의 dep 힘 제어 및 전기습윤 제어
CN103926190A (zh) * 2014-05-08 2014-07-16 齐鲁工业大学 基于微流控系统的单细胞自动分析方法
JP2016217888A (ja) * 2015-05-20 2016-12-22 シスメックス株式会社 細胞検出装置および細胞検出方法

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114088588A (zh) * 2021-10-27 2022-02-25 西安理工大学 一种基于无透镜成像的红细胞三维尺寸测量方法
CN114088588B (zh) * 2021-10-27 2024-04-09 西安理工大学 一种基于无透镜成像的红细胞三维尺寸测量方法

Also Published As

Publication number Publication date
JP2024020465A (ja) 2024-02-14
GB202011401D0 (en) 2020-09-09
AU2023286012A1 (en) 2024-01-25
JP2021515239A (ja) 2021-06-17
BR112020012748A2 (pt) 2020-12-01
CA3086498A1 (en) 2019-06-27
AU2017443695A1 (en) 2020-07-09
GB2584218A (en) 2020-11-25
EP3728107A4 (en) 2021-08-04
KR20230157541A (ko) 2023-11-16
KR20200104354A (ko) 2020-09-03
SG11202005872XA (en) 2020-07-29
JP7390305B2 (ja) 2023-12-01
EP3728107A1 (en) 2020-10-28
WO2019125502A1 (en) 2019-06-27
KR102602599B1 (ko) 2023-11-16
MX2020007182A (es) 2020-08-24
AU2017443695B2 (en) 2023-09-28
RU2764676C1 (ru) 2022-01-19

Similar Documents

Publication Publication Date Title
US11561164B2 (en) Microfluidic chip device for optical force measurements and cell imaging using microfluidic chip configuration and dynamics
US11714044B2 (en) Micro-lens systems for particle processing systems
US10107735B2 (en) System and method for deforming and analyzing particles
US8691164B2 (en) Cell sorting system and methods
AU2023286012A1 (en) Microfluidic chip device for optical force measurements and cell imaging using microfluidic chip configuration and dynamics
US20170299492A1 (en) Method, device and system for hydrodynamic flow focusing
US11913870B2 (en) Microfluidic chip device for optical force measurements and cell imaging using microfluidic chip configuration and dynamics
US9529203B2 (en) Focal plane shifting system
US20160011114A1 (en) Photometric analysis method and photometric analysis device using microchip, microchip for photometric analysis device, and processing device for photometric analysis
ES2644854T3 (es) Unidad que comprende un conjunto de contenedores de muestra
US11686662B2 (en) Microparticle sorting device and method for sorting microparticles
TW202035969A (zh) 用於光學力測量之微流體晶片裝置及使用微流體晶片組態及動力學之細胞造影
US20220388004A1 (en) Systems and methods for multi-junction particle sorting in injection-molded articles
US20240024867A1 (en) Flowcell holder and biological analysis systems and methods
CN101743303B (zh) 细胞分选系统和方法

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination