AU2017443695B2 - Microfluidic chip device for optical force measurements and cell imaging using microfluidic chip configuration and dynamics - Google Patents

Microfluidic chip device for optical force measurements and cell imaging using microfluidic chip configuration and dynamics Download PDF

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Publication number
AU2017443695B2
AU2017443695B2 AU2017443695A AU2017443695A AU2017443695B2 AU 2017443695 B2 AU2017443695 B2 AU 2017443695B2 AU 2017443695 A AU2017443695 A AU 2017443695A AU 2017443695 A AU2017443695 A AU 2017443695A AU 2017443695 B2 AU2017443695 B2 AU 2017443695B2
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channel
substrate
substance
chip
imaging
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AU2017443695A1 (en
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Christopher Field
Sean Hart
Colin HEBERT
Shweta KRISHNAN
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Lumacyte LLC
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Lumacyte LLC
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    • BPERFORMING OPERATIONS; TRANSPORTING
    • B81MICROSTRUCTURAL TECHNOLOGY
    • B81BMICROSTRUCTURAL DEVICES OR SYSTEMS, e.g. MICROMECHANICAL DEVICES
    • B81B1/00Devices without movable or flexible elements, e.g. microcapillary devices
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Electro-optical investigation, e.g. flow cytometers
    • G01N15/1434Electro-optical investigation, e.g. flow cytometers using an analyser being characterised by its optical arrangement
    • G01N15/1436Electro-optical investigation, e.g. flow cytometers using an analyser being characterised by its optical arrangement the optical arrangement forming an integrated apparatus with the sample container, e.g. a flow cell
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Electro-optical investigation, e.g. flow cytometers
    • G01N15/1484Electro-optical investigation, e.g. flow cytometers microstructural devices
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03Cuvette constructions
    • G01N21/05Flow-through cuvettes
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/08Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06Fluid handling related problems
    • B01L2200/0647Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0663Stretching or orienting elongated molecules or particles
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06Fluid handling related problems
    • B01L2200/0647Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0668Trapping microscopic beads
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/08Geometry, shape and general structure
    • B01L2300/0861Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0874Three dimensional network
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00Moving or stopping fluids
    • B01L2400/04Moving fluids with specific forces or mechanical means
    • B01L2400/0403Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0454Moving fluids with specific forces or mechanical means specific forces radiation pressure, optical tweezers
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00Moving or stopping fluids
    • B01L2400/04Moving fluids with specific forces or mechanical means
    • B01L2400/0475Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00Moving or stopping fluids
    • B01L2400/04Moving fluids with specific forces or mechanical means
    • B01L2400/0475Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B01L2400/049Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics vacuum
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Electro-optical investigation, e.g. flow cytometers
    • G01N15/1468Electro-optical investigation, e.g. flow cytometers with spatial resolution of the texture or inner structure of the particle
    • G01N15/147Electro-optical investigation, e.g. flow cytometers with spatial resolution of the texture or inner structure of the particle the analysis being performed on a sample stream
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N2015/1006Investigating individual particles for cytology

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Fluid Mechanics (AREA)
  • Engineering & Computer Science (AREA)
  • Computer Hardware Design (AREA)
  • Microelectronics & Electronic Packaging (AREA)
  • Optical Measuring Cells (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
AU2017443695A 2017-12-23 2017-12-23 Microfluidic chip device for optical force measurements and cell imaging using microfluidic chip configuration and dynamics Active AU2017443695B2 (en)

Priority Applications (1)

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AU2023286012A AU2023286012A1 (en) 2017-12-23 2023-12-27 Microfluidic chip device for optical force measurements and cell imaging using microfluidic chip configuration and dynamics

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/US2017/068373 WO2019125502A1 (en) 2017-12-23 2017-12-23 Microfluidic chip device for optical force measurements and cell imaging using microfluidic chip configuration and dynamics

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AU2023286012A Division AU2023286012A1 (en) 2017-12-23 2023-12-27 Microfluidic chip device for optical force measurements and cell imaging using microfluidic chip configuration and dynamics

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AU2017443695A1 AU2017443695A1 (en) 2020-07-09
AU2017443695B2 true AU2017443695B2 (en) 2023-09-28

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AU2023286012A Pending AU2023286012A1 (en) 2017-12-23 2023-12-27 Microfluidic chip device for optical force measurements and cell imaging using microfluidic chip configuration and dynamics

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Country Status (12)

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EP (1) EP3728107A4 (ru)
JP (2) JP7390305B2 (ru)
KR (2) KR102602599B1 (ru)
CN (1) CN111819153A (ru)
AU (2) AU2017443695B2 (ru)
BR (1) BR112020012748A2 (ru)
CA (1) CA3086498A1 (ru)
GB (1) GB2584218A (ru)
MX (1) MX2020007182A (ru)
RU (1) RU2764676C1 (ru)
SG (1) SG11202005872XA (ru)
WO (1) WO2019125502A1 (ru)

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KR102656008B1 (ko) * 2020-09-08 2024-04-11 경북대학교 산학협력단 질병 진단용 키트, 질병 진단용 키트를 이용한 질병 진단 방법 및 질병 진단용 키트의 제조 방법
CN112986063B (zh) * 2021-01-18 2022-04-15 西北大学 高通量染色体和细胞骨架应变流式分析仪及实施方法
CN114088588B (zh) * 2021-10-27 2024-04-09 西安理工大学 一种基于无透镜成像的红细胞三维尺寸测量方法

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US20050002025A1 (en) * 2003-05-15 2005-01-06 Toshiba Ceramics Co., Ltd. Channel structure and process for production thereof
US7068874B2 (en) * 2000-11-28 2006-06-27 The Regents Of The University Of California Microfluidic sorting device
US20090140170A1 (en) * 2005-08-11 2009-06-04 Eksigent Technologies, Llc Microfluidic systems, devices and methods for reducing background autofluorescence and the effects thereof
US20140085898A1 (en) * 2012-09-17 2014-03-27 Cytonome/St, Llc Focal plane shifting system
US20140193892A1 (en) * 2012-07-25 2014-07-10 Theranos, Inc. Image analysis and measurement of biological samples
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US7068874B2 (en) * 2000-11-28 2006-06-27 The Regents Of The University Of California Microfluidic sorting device
US20050002025A1 (en) * 2003-05-15 2005-01-06 Toshiba Ceramics Co., Ltd. Channel structure and process for production thereof
US20090140170A1 (en) * 2005-08-11 2009-06-04 Eksigent Technologies, Llc Microfluidic systems, devices and methods for reducing background autofluorescence and the effects thereof
US20140220557A1 (en) * 2007-12-21 2014-08-07 Sean J. Hart Device and Method for Laser Analysis and Separation (LAS) of Particles
US20150316188A1 (en) * 2012-06-29 2015-11-05 University Of South Australia Fluid connection ports
US20140193892A1 (en) * 2012-07-25 2014-07-10 Theranos, Inc. Image analysis and measurement of biological samples
US20140085898A1 (en) * 2012-09-17 2014-03-27 Cytonome/St, Llc Focal plane shifting system

Also Published As

Publication number Publication date
JP2024020465A (ja) 2024-02-14
GB202011401D0 (en) 2020-09-09
AU2023286012A1 (en) 2024-01-25
JP2021515239A (ja) 2021-06-17
BR112020012748A2 (pt) 2020-12-01
CN111819153A (zh) 2020-10-23
CA3086498A1 (en) 2019-06-27
AU2017443695A1 (en) 2020-07-09
GB2584218A (en) 2020-11-25
EP3728107A4 (en) 2021-08-04
KR20230157541A (ko) 2023-11-16
KR20200104354A (ko) 2020-09-03
SG11202005872XA (en) 2020-07-29
JP7390305B2 (ja) 2023-12-01
EP3728107A1 (en) 2020-10-28
WO2019125502A1 (en) 2019-06-27
KR102602599B1 (ko) 2023-11-16
MX2020007182A (es) 2020-08-24
RU2764676C1 (ru) 2022-01-19

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