CN111802510A - Animal waste fermented organic feed and preparation method thereof - Google Patents

Animal waste fermented organic feed and preparation method thereof Download PDF

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Publication number
CN111802510A
CN111802510A CN202010704246.2A CN202010704246A CN111802510A CN 111802510 A CN111802510 A CN 111802510A CN 202010704246 A CN202010704246 A CN 202010704246A CN 111802510 A CN111802510 A CN 111802510A
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parts
feed
animal
organic feed
wastes
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黄俊文
林坤志
邓良敦
邓武敦
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Kunshan Liqing Biotechnology Co ltd
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/10Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/12Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/20Animal feeding-stuffs from material of animal origin
    • A23K10/26Animal feeding-stuffs from material of animal origin from waste material, e.g. feathers, bones or skin
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/90Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor
    • C12N1/16Yeasts; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor

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  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
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  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Animal Husbandry (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Physiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
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Abstract

The invention discloses an animal waste fermented organic feed and a preparation method thereof, wherein the organic feed comprises the following components in parts by weight: the fermented milk comprises 50 parts of animal wastes, 50 parts of dried substances and 0.1 part of ferment, wherein the ferment comprises 25 parts of potato scab germs, 10 parts of lactobacillus casei, 10 parts of wood acetic acid bacteria, 15 parts of saccharomyces cerevisiae, 10 parts of bacillus subtilis and 15 parts of bifidobacterium. The organic feed contains various high-quality lactic acid bacteria, can effectively degrade anti-nutritional factors in the feed, improves the nutritional value and the digestibility of the feed, improves the flavor and the palatability of the feed, effectively prevents the feed from mildewing and the production of aflatoxin, ensures the safety of the fermented feed, avoids the loss of nutrition and weight caused by the production of heat and gas by fermentation, and can simultaneously achieve the purposes of recycling, reducing and harmlessness of animal wastes by fermenting the animal wastes with the organic feed to breed hermetia illucens.

Description

Animal waste fermented organic feed and preparation method thereof
Technical Field
The invention relates to an animal manure fermented organic feed and a preparation method thereof, belonging to the technical field of microbial fermentation.
Background
With the large-scale and intensive development of livestock and poultry breeding industry in China, the problem of fecal pollution is more serious, and the difficulty in fecal pollution management is increased. The anaerobic biotechnology is one of effective means for the resource management of the excrement in the breeding industry, has the advantages of generating bioenergy, low demand on nitrogen and phosphorus nutrient elements, good degradation capability on certain organic matters and the like, and is widely applied to the treatment of the breeding excrement at present. The construction of large and medium-sized biogas projects in China is on a rapid growth trend, and the biogas projects are expected to cover 84% of the existing large and medium-sized animal farms in 2020. In recent years, the central first-grade encourages the development of the biogas industry, but the anaerobic biotechnology does not have the functions of nitrogen and phosphorus removal and recovery, so that anaerobic fermentation liquor, a byproduct of biogas engineering, is easy to become a secondary pollution source, and for breeding enterprises with insufficient land consumption at the periphery, the biogas engineering cannot completely realize the dual purposes of pollution control and profit.
Disclosure of Invention
The organic feed contains various high-quality lactic acid bacteria, can effectively degrade antinutritional factors in the feed, improves the nutritional value and the digestibility of the feed, improves the flavor and the palatability of the feed, effectively prevents the feed from mildewing and the generation of aflatoxin, ensures the safety of the fermented feed, avoids the loss of nutrition and weight caused by heat and gas production due to fermentation, and can simultaneously achieve the aims of recycling, reducing and harmlessness of animal excrements by utilizing the animal excrements to ferment the organic feed to breed the hermetia illucens.
In order to solve the technical problems, the invention provides an animal manure fermented organic feed which comprises the following components in parts by weight: 50 parts of animal excrement, 50 parts of dried substances and 0.1 part of ferment, wherein the ferment comprises potato scab bacteria (A)Streptomyces Turgidiscabies) 25 parts of lactobacillus casei (lactobacillus casei) (III)Lactobacillus casei) 10 parts of Acetobacter xylinum (A)Acetobacter xylinum) 10 portions of saccharomyces cerevisiae (Saccharomyces cerevisiae) 15 parts of Bacillus subtilis (Bacillus subtilis) ((B))Bacillus subtilis) 10 parts of bifidobacteriumBifidobacterium bifidum) 15 parts.
Preferably, the additive also comprises molasses accounting for 10-30% of the total weight.
Preferably, the trace element-containing copper-magnesium alloy further comprises one or more of copper, magnesium, zinc, iron, boron, calcium and manganese, wherein the content of each trace element is one thousandth of the total weight.
Preferably, the mineral composition also comprises minerals, wherein the minerals comprise one or more of calcium, chlorine, magnesium, phosphorus, potassium, sodium and sulfur, and the content of each mineral is one thousandth of the total weight.
Preferably, the animal manure is one or more of pig manure, chicken manure, duck manure, cattle manure and sheep manure.
The invention also provides a preparation method of the animal waste fermented organic feed, which comprises the following steps:
preparing a culture medium stock solution, extracting a part of the stock solution to a new barrel, adding molasses and water into the new barrel, and uniformly stirring;
pouring any five of potato scab bacteria, lactobacillus casei, acetobacter xylinum, saccharomyces cerevisiae, bacillus subtilis and bifidobacterium strains into a glass bottle respectively, adding water, and uniformly shaking until bubbles are generated;
taking out part of potato scab bacteria 25ML, lactobacillus casei 10ML, acetobacter xylinum 10ML, saccharomyces cerevisiae 15ML, bacillus subtilis 10ML and bifidobacterium 15ML from each glass bottle, putting into a container, uniformly shaking until bubbles are generated, standing, and pouring into a new barrel;
pouring 25ML of potato scab germs, 15ML of saccharomyces cerevisiae and 15ML of bifidobacteria into the new barrel, and uniformly stirring and aerating to complete a new barrel of culture medium;
pouring lactobacillus casei 10ML, acetobacter xylinum 10ML and bacillus subtilis 10ML into the new barrel of culture medium, uniformly stirring and aerating to obtain a finished enzyme product;
collecting animal excrement water, adding the enzyme finished product into the animal excrement water, stirring and mixing uniformly, standing for 3-5 days, taking 50 parts of excrement, 50 parts of dried material and 0.1 part of enzyme finished product, mixing, finely crushing, granulating, obtaining the finished product, and packaging.
Preferably, the preparation method of the culture medium stock solution comprises the following steps:
mixing 40-60% of water and 10-30% of molasses according to the weight percentage, and then uniformly stirring;
adding water into each strain to adjust the weight of the strain to 4-6% of the total weight, wherein the weight of the strain is 25ML of potato scab bacteria, 10ML of lactobacillus casei, 10ML of acetobacter xylinum, 15ML of saccharomyces cerevisiae, 10ML of bacillus subtilis and 15ML of bifidobacterium;
and aeration observation is carried out to form a culture medium stock solution.
Preferably, the stock solution comprises 50% of the total weight of the new keg.
Preferably, molasses accounting for 10-30% of the total weight and water accounting for 20-40% of the total weight are added into the new barrel.
Preferably, 40-60 ml of any five strains are taken and poured into a glass bottle respectively, and then 400-600 ml of water is added and then the mixture is uniformly shaken.
The invention achieves the following beneficial effects:
(1) the feed treated by fermentation not only has the functions of improving the nutrient absorption level of the feed, degrading possible toxins in feed raw materials, but also can greatly reduce the use of pharmaceutical additives such as antibiotics and the like, and improve the health level of animals, thereby improving the food safety of products.
(2) After the feed is fermented, a series of biochemical reactions are carried out, complex macromolecular organic matters such as cellulose, starch, protein and the like in the feed are degraded into micromolecular substances such as monosaccharide, disaccharide, oligosaccharide, amino acid and the like which are easy to digest and absorb by animals to a certain extent, so that the digestibility of the feed is improved, the deep production and processing effects which cannot be achieved by feed machinery are achieved, meanwhile, a large number of microbial strain cells with rich nutrition and useful metabolites such as amino acid, organic acid, alcohol, aldehyde, ester, vitamin, hormone, activated trace elements and special carbohydrate are generated and accumulated in the process of feed fermentation, the feed is softened and flavored, the nutrition is increased, the physicochemical properties of the feed are changed, and the palatability, the digestibility, the absorptivity and the nutritional value of the feed are improved.
(3) The lactobacillus can inhibit the growth and toxin production of mould, and the lactobacillus acidophilus, the lactobacillus bulgaricus and the lactobacillus plantarum can inhibit the spore germination of aspergillus parasiticus. The metabolite of the microorganism can also reduce the toxin content in the feed, for example, mannan can effectively degrade aspergillus flavus and the like. The growth promoting factors generated by fermenting the feed by different strains have different contents, mainly comprise organic acid, B vitamins, unknown growth factors and the like, and can promote the growth of livestock.
(4) The invention contains various high-quality lactic acid bacteria, can effectively degrade antinutritional factors in the feed, improve the nutritional value and digestibility of the feed, improve the flavor and palatability of the feed, effectively prevent the feed from mildewing and aflatoxin generation, ensure the safety of fermented feed and avoid the loss of nutrition and weight caused by heat and gas production due to fermentation.
(5) The black soldier fly breeding cycle is mostly 32 days, and the relatively short breeding cycle does benefit to the quick turnover that reduces area in ground and equipment quantity and fund, can reduce the breed risk. The hermetia illucens has high conversion rate of feces, and feces treated by the hermetia illucens can be used as an organic fertilizer for application. The method can achieve the purposes of recycling, reducing and harmlessness of the animal wastes at the same time by fermenting the animal wastes with the organic feed to breed the hermetia illucens.
Detailed Description
The invention is further described below. The following examples are only for illustrating the technical solutions of the present invention more clearly, and the protection scope of the present invention is not limited thereby.
Example 1
The invention provides an animal manure fermented organic feed, which comprises the following components in percentage by weight:
the fermented milk comprises 50 parts of animal wastes, 50 parts of dried substances and 0.1 part of ferment, wherein the ferment comprises 25 parts of potato scab germs, 10 parts of lactobacillus casei, 10 parts of wood acetic acid bacteria, 15 parts of saccharomyces cerevisiae, 10 parts of bacillus subtilis and 15 parts of bifidobacterium.
The invention also comprises molasses with the total weight of 10-30%, and trace elements and minerals.
The trace elements comprise one or more of copper, magnesium, zinc, iron, boron, calcium and manganese, and the content of each trace element is one thousandth of the total weight.
The mineral substances comprise one or more of calcium, chlorine, magnesium, phosphorus, potassium, sodium and sulfur, and the content of each mineral substance is one thousandth of the total weight.
The invention also provides a preparation method of the animal waste fermented organic feed, which comprises the following steps:
(1) mixing 40-60% of water and 10-30% of molasses according to the weight percentage, and then uniformly stirring;
(2) adding water into each strain to adjust the weight of the strain to 4-6% of the total weight, wherein the weight of the strain is 25ML of potato scab bacteria, 10ML of lactobacillus casei, 10ML of acetobacter xylinum, 15ML of saccharomyces cerevisiae, 10ML of bacillus subtilis and 15ML of bifidobacterium;
(3) aeration observation is carried out to form a culture medium stock solution;
(4) pre-mixing a culture medium stock solution, and extracting part of the stock solution to a new barrel to ensure that the stock solution accounts for 50% of the total weight of the new barrel;
(5) adding molasses accounting for 10-30% of the total weight and water accounting for 20-40% of the total weight into the new barrel, and uniformly stirring;
(6) taking 40-60 ml of each of five strains from potato scab bacteria, lactobacillus casei, acetobacter xylinum, saccharomyces cerevisiae, bacillus subtilis and bifidobacterium strains, pouring the five strains into a glass bottle respectively, adding 400-600 ml of water, and uniformly shaking until bubbles are generated;
(7) taking 500 ml of the five strains out of each glass bottle, putting the five strains into a container, uniformly shaking the five strains until bubbles are generated, standing the five strains for a proper time, and pouring the five strains into a new barrel;
(8) pouring 100-300 ml of the strain with specific required function into the new barrel, and stirring and aerating to complete the culture medium in the new barrel;
(9) pouring 100-300 ml of strain with specific required function into the new barrel culture medium, stirring and aerating to complete the finished enzyme product, and adding trace elements and minerals into the finished enzyme product;
(10) collecting pig manure water, adding the enzyme finished product into the pig manure water, stirring and mixing uniformly, standing for 3-5 days, taking 50 parts of manure, 50 parts of dried material and 0.1 part of enzyme finished product, mixing, finely crushing, granulating, obtaining the finished product, and packaging.
Example 2
The pig manure fermented organic feed prepared in example 1 is used for breeding hermetia illucens.
The large-scale pig farms in China generally feed live pigs by using diets with balanced nutrition, pig manure contains a large amount of unabsorbed organic matters, mineral substances, vitamins and the like, and the fresh pig manure with rich nutrition can be directly used as a feeding base for hermetia illucens. Utilize fresh pig manure to breed heisui river horsefly, can save manufacturing cost, pig manure low price moreover, because do not have enough soil to carry out the excrement and dirt and consume, the dry excrement of a lot of pig farms is for the use of surrounding peasant household for gratuity, and partial pig farm goes out the transportation charge even and lets the grower transport away the pig farm excrement and dirt, compares, heisui river horsefly's breed cost is cheaper. The earthworm cultivation period is generally 4-5 months, the hermetia illucens cultivation period is mostly 32 days, the relatively short cultivation period is favorable for reducing the area of a field and the rapid turnover of the quantity of equipment and capital, and the cultivation risk can be reduced. The hermetia illucens has high conversion rate of manure, and the pig manure treated by the hermetia illucens can be used as an organic fertilizer. The purposes of recycling, reducing and harmlessly treating the pig manure can be simultaneously achieved by utilizing the pig manure to culture the hermetia illucens.
The above description is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, several modifications and variations can be made without departing from the technical principle of the present invention, and these modifications and variations should also be regarded as the protection scope of the present invention.

Claims (10)

1. An animal waste fermented organic feed is characterized by comprising the following components in parts by weight: the fermented milk comprises 50 parts of animal wastes, 50 parts of dried substances and 0.1 part of ferment, wherein the ferment comprises 25 parts of potato scab germs, 10 parts of lactobacillus casei, 10 parts of wood acetic acid bacteria, 15 parts of saccharomyces cerevisiae, 10 parts of bacillus subtilis and 15 parts of bifidobacterium.
2. The animal waste fermented organic feed according to claim 1, further comprising molasses in an amount of 10 to 30% by weight based on the total amount.
3. The fermented organic animal manure feed of claim 1, further comprising trace elements including one or more of copper, magnesium, zinc, iron, boron, calcium and manganese, each trace element being present in a thousandth of the total weight.
4. The animal manure fermented organic feed of claim 1, further comprising minerals including one or more of calcium, chlorine, magnesium, phosphorus, potassium, sodium, sulfur, each mineral being present in one thousandth of the total weight.
5. The fermented organic feed of animal wastes according to claim 1, wherein the animal wastes are one or more of pig wastes, chicken wastes, duck wastes, cow wastes and sheep wastes.
6. The preparation method of the animal waste fermented organic feed is characterized by comprising the following steps:
preparing a culture medium stock solution, extracting a part of the stock solution to a new barrel, adding molasses and water into the new barrel, and uniformly stirring;
pouring any five of potato scab bacteria, lactobacillus casei, acetobacter xylinum, saccharomyces cerevisiae, bacillus subtilis and bifidobacterium strains into a glass bottle respectively, adding water, and uniformly shaking until bubbles are generated;
taking out part of potato scab bacteria 25ML, lactobacillus casei 10ML, acetobacter xylinum 10ML, saccharomyces cerevisiae 15ML, bacillus subtilis 10ML and bifidobacterium 15ML from each glass bottle, putting into a container, uniformly shaking until bubbles are generated, standing, and pouring into a new barrel;
pouring 25ML of potato scab germs, 15ML of saccharomyces cerevisiae and 15ML of bifidobacteria into the new barrel, and uniformly stirring and aerating to complete a new barrel of culture medium;
pouring lactobacillus casei 10ML, acetobacter xylinum 10ML and bacillus subtilis 10ML into the new barrel of culture medium, uniformly stirring and aerating to obtain a finished enzyme product;
collecting animal excrement water, adding the enzyme finished product into the animal excrement water, stirring and mixing uniformly, standing for 3-5 days, taking 50 parts of excrement, 50 parts of dried material and 0.1 part of enzyme finished product, mixing, finely crushing, granulating, obtaining the finished product, and packaging.
7. The method for preparing an animal waste fermented organic feed according to claim 6, wherein the method for preparing the culture medium stock solution comprises:
mixing 40-60% of water and 10-30% of molasses according to the weight percentage, and then uniformly stirring;
adding water into each strain to adjust the weight of the strain to 4-6% of the total weight, wherein the weight of the strain is 25ML of potato scab bacteria, 10ML of lactobacillus casei, 10ML of acetobacter xylinum, 15ML of saccharomyces cerevisiae, 10ML of bacillus subtilis and 15ML of bifidobacterium;
and aeration observation is carried out to form a culture medium stock solution.
8. The method for preparing an animal manure fermented organic feed as claimed in claim 6, wherein the stock solution is 50% of the total weight of the new barrel.
9. The method for preparing the animal waste fermented organic feed according to claim 6, wherein 10-30% of molasses and 20-40% of water by weight are added into the new barrel.
10. The method for preparing the fermented organic feed of the animal wastes according to claim 6, wherein 40 to 60 ml of any five strains are respectively taken and poured into a glass bottle, and then 400 to 600 ml of water is added and uniformly shaken.
CN202010704246.2A 2020-07-21 2020-07-21 Animal waste fermented organic feed and preparation method thereof Pending CN111802510A (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2764264C1 (en) * 2021-03-15 2022-01-14 Федеральное государственное бюджетное образовательное учреждение высшего образования "Новосибирский государственный аграрный университет" Feed additive for honey bees
CN114437988A (en) * 2022-02-28 2022-05-06 贵州医科大学 Cellulose degrading bacteria, microbial inoculum, preparation method and application thereof, and method for degrading vinasse
CN115024448A (en) * 2022-06-15 2022-09-09 贵州大学 Method for preparing hermetia illucens enzyme feed by using waste vegetables and fruits

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CN101628841A (en) * 2009-08-19 2010-01-20 江苏亿海肥业有限公司 Method for producing organic and inorganic compound fertilizer by utilizing duck dropping source
JP2011142896A (en) * 2010-01-12 2011-07-28 Oobiken:Kk Method for producing organic fermented feed
CN104256181A (en) * 2014-10-10 2015-01-07 青岛嘉瑞生物技术有限公司 Process for producing pig compound feed by resource utilization of dairy cow manure
TW201619372A (en) * 2014-11-25 2016-06-01 黃俊文 Culture medium's replication method
CN106614398A (en) * 2016-12-19 2017-05-10 江苏大学 Method for performing pig manure resource treatment by utilizing hermetia illucens
CN107354193A (en) * 2016-05-09 2017-11-17 黄俊文 The clone method of culture medium

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101628841A (en) * 2009-08-19 2010-01-20 江苏亿海肥业有限公司 Method for producing organic and inorganic compound fertilizer by utilizing duck dropping source
JP2011142896A (en) * 2010-01-12 2011-07-28 Oobiken:Kk Method for producing organic fermented feed
CN104256181A (en) * 2014-10-10 2015-01-07 青岛嘉瑞生物技术有限公司 Process for producing pig compound feed by resource utilization of dairy cow manure
TW201619372A (en) * 2014-11-25 2016-06-01 黃俊文 Culture medium's replication method
CN107354193A (en) * 2016-05-09 2017-11-17 黄俊文 The clone method of culture medium
CN106614398A (en) * 2016-12-19 2017-05-10 江苏大学 Method for performing pig manure resource treatment by utilizing hermetia illucens

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2764264C1 (en) * 2021-03-15 2022-01-14 Федеральное государственное бюджетное образовательное учреждение высшего образования "Новосибирский государственный аграрный университет" Feed additive for honey bees
CN114437988A (en) * 2022-02-28 2022-05-06 贵州医科大学 Cellulose degrading bacteria, microbial inoculum, preparation method and application thereof, and method for degrading vinasse
CN114437988B (en) * 2022-02-28 2023-05-23 贵州医科大学 Cellulose degrading bacteria and microbial inoculum, preparation method, application and method for degrading distillers' grains
CN115024448A (en) * 2022-06-15 2022-09-09 贵州大学 Method for preparing hermetia illucens enzyme feed by using waste vegetables and fruits

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Application publication date: 20201023