CN111802334A - Sterilization and disinfection method in artificial culture of wild giant silkworm - Google Patents
Sterilization and disinfection method in artificial culture of wild giant silkworm Download PDFInfo
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- CN111802334A CN111802334A CN202010822907.1A CN202010822907A CN111802334A CN 111802334 A CN111802334 A CN 111802334A CN 202010822907 A CN202010822907 A CN 202010822907A CN 111802334 A CN111802334 A CN 111802334A
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- sterilization
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- folium artemisiae
- artemisiae argyi
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- 241000255789 Bombyx mori Species 0.000 title claims abstract description 62
- 238000004659 sterilization and disinfection Methods 0.000 title claims abstract description 62
- 238000000034 method Methods 0.000 title claims abstract description 27
- 241000255794 Bombyx mandarina Species 0.000 claims abstract description 33
- 241000257303 Hymenoptera Species 0.000 claims abstract description 33
- 239000012286 potassium permanganate Substances 0.000 claims abstract description 21
- 238000009395 breeding Methods 0.000 claims abstract description 13
- 230000001488 breeding effect Effects 0.000 claims abstract description 12
- 238000005507 spraying Methods 0.000 claims abstract description 11
- 230000001954 sterilising effect Effects 0.000 claims description 28
- 239000007788 liquid Substances 0.000 claims description 14
- 230000000249 desinfective effect Effects 0.000 claims description 11
- 238000009928 pasteurization Methods 0.000 claims description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 9
- 238000003958 fumigation Methods 0.000 claims description 8
- ODINCKMPIJJUCX-UHFFFAOYSA-N Calcium oxide Chemical compound [Ca]=O ODINCKMPIJJUCX-UHFFFAOYSA-N 0.000 claims description 6
- 230000009545 invasion Effects 0.000 claims description 6
- 238000009423 ventilation Methods 0.000 claims description 6
- 239000004677 Nylon Substances 0.000 claims description 4
- 206010070834 Sensitisation Diseases 0.000 claims description 4
- 229920001778 nylon Polymers 0.000 claims description 4
- 238000000746 purification Methods 0.000 claims description 4
- 230000008313 sensitization Effects 0.000 claims description 4
- 241000258920 Chilopoda Species 0.000 claims description 3
- 235000012255 calcium oxide Nutrition 0.000 claims description 3
- 239000000292 calcium oxide Substances 0.000 claims description 3
- 238000001816 cooling Methods 0.000 claims description 3
- 230000003203 everyday effect Effects 0.000 claims description 3
- 238000001914 filtration Methods 0.000 claims description 3
- 230000002265 prevention Effects 0.000 claims description 3
- 239000000779 smoke Substances 0.000 claims description 3
- 239000002689 soil Substances 0.000 claims description 3
- 241000256602 Isoptera Species 0.000 claims 1
- 239000000203 mixture Substances 0.000 claims 1
- 239000008213 purified water Substances 0.000 claims 1
- 239000006228 supernatant Substances 0.000 claims 1
- 230000004083 survival effect Effects 0.000 abstract description 5
- 230000002093 peripheral effect Effects 0.000 description 8
- 241000238631 Hexapoda Species 0.000 description 3
- 241000660877 Coridius Species 0.000 description 2
- 229920000433 Lyocell Polymers 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 241001481304 Vespoidea Species 0.000 description 2
- 244000052616 bacterial pathogen Species 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 230000000391 smoking effect Effects 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 208000035473 Communicable disease Diseases 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000002932 luster Substances 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 230000000384 rearing effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
- A01K67/04—Silkworms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K40/00—Shaping or working-up of animal feeding-stuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/90—Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Environmental Sciences (AREA)
- Animal Husbandry (AREA)
- Engineering & Computer Science (AREA)
- Birds (AREA)
- Animal Behavior & Ethology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Insects & Arthropods (AREA)
- Catching Or Destruction (AREA)
Abstract
The invention discloses a sterilization and disinfection method in artificial breeding of wild silkworm, which comprises a food sterilization and disinfection step, wherein the food sterilization and disinfection step is to regularly sterilize and disinfect the bred wild silkworm edible tree and edible tree leaves before the emergence of ants, the sterilization and disinfection is carried out by spraying potassium permanganate solution, and then the sterilization and disinfection is carried out by spraying folium artemisiae argyi solution. The invention can obviously improve the survival rate of the giant silkworm.
Description
Technical Field
The invention belongs to a silkworm breeding method, and particularly relates to a sterilization and disinfection method in artificial breeding of wild silkworm.
Background
Since the last 80 years, people are always researching how to introduce wild silkworms into domestic rearing for industrial development so as to bring benefits to human better. However, because the wild silkworms have unique habits, the survival rate of the wild silkworms in the artificial breeding process is hardly over 15 percent, so that the good desire cannot be realized until now.
Disclosure of Invention
The invention aims to provide a sterilization and disinfection method for artificial culture of wild silkworm, which can improve the survival rate.
The technical scheme adopted for realizing the purpose of the invention is as follows:
the sterilization and disinfection method for artificial breeding of wild silkworm comprises a food sterilization and disinfection step, wherein the food sterilization and disinfection step is to regularly sterilize and disinfect the bred wild silkworm edible tree and edible tree leaves before the ants emerge, and the sterilization and disinfection is carried out by spraying potassium permanganate solution and then spraying folium artemisiae argyi solution.
The regular sterilization and disinfection is to perform fixed-point and fixed-stump sterilization and disinfection on the silkworm feeding trees and the leaf feeding trees every two days five days before the ants come out.
The potassium permanganate solution is sprayed to sterilize, and the solid potassium permanganate and the water purifying solution are prepared according to the ratio of 2-5: 1000-1800, and the potassium permanganate solution is sprayed to the root from the upper end of the main root of the single root edible tree.
The folium artemisiae argyi liquid is sprayed with the folium artemisiae argyi liquid to sterilize, and the folium artemisiae argyi liquid is prepared by decocting dry folium artemisiae argyi and a water purification solution according to a ratio of 1: 10-20 to 10-13 kg with fire, filtering the folium artemisiae argyi after cooling, adding 150-240 kg of water, and spraying the upper layer liquid on the branch extending and the branch extending leaves of the single-stump edible tree to sterilize.
The method also comprises an outdoor environment sterilization step, wherein the outdoor environment sterilization step is to sterilize and disinfect the environment around the silkworm house by using medical pasteurization liquid and potassium permanganate solution before and after the ants come out.
The sterilization and disinfection of the silkworm house peripheral environment by the medicinal pasteurization liquid and the potassium permanganate solution before and after the emergence of the ants are respectively realized by disinfecting the silkworm house peripheral environment twice by the medicinal pasteurization liquid three days before the emergence of the ants and disinfecting the silkworm house peripheral environment by the potassium permanganate solution with the concentration of two thousandths to five thousandths of a day every 2 days after the emergence of the ants until the silkworm house peripheral environment is five-year old.
The invention also comprises a disinfection and sterilization step for the indoor culture of the wild silkworms, wherein the disinfection and sterilization step for the indoor culture of the wild silkworms is to close the door and window before and after the ants come out and smoke the dried folium artemisiae argyi.
The door and window is smoked by using the dry folium artemisiae argyi three days before the emergence of the ants, the door and window are closed by using the dry folium artemisiae argyi for 10 to 20 minutes every day, and the door and window are opened for ventilation one day before the emergence of the ants;
the door and window is closed after the ants come out for 5 to 7 days, the door and window is closed after the ants come out, the folium artemisiae argyi is used for fumigation, sterilization and disinfection for 10 to 20 minutes, then the door and window are opened for ventilation, the method is carried out until the silkworms enter into the third-instar stage and are moved to outdoor cultivation, and if the silkworms of 4 to 5 instars are cultivated indoors until cocoons are formed, the method is also used for operation.
The screen door and window are additionally arranged on the door and window to prevent insects such as parasitic wasps, aspongopus and the like from invading the door and window to infringe the tencel silkworms.
The invention also comprises a disinfection and sterilization step for the wild silkworm outdoor culture, wherein the disinfection and sterilization step for the wild silkworm outdoor culture is that young silkworms are moved to the outdoor culture after entering the third-instar period, and all single-stump edible trees eaten by the silkworms are fumigated and sterilized by folium artemisiae argyi; a circle of quicklime is scattered around the young silkworm on the periphery of the feeding single-stump feeding tree, so that the aim of sterilizing and disinfecting soil is fulfilled, and the invasion of centipede worms to the wild silkworm is prevented; the giant silkworm is covered by a nylon net for sensitization and prevention of the invasion of birds to the giant silkworm.
The regular folium artemisiae argyi fumigation and sterilization is to fumigate and sterilize folium artemisiae argyi every 4 to 6 days for 10 to 20 minutes.
The fumigation and sterilization with the folium artemisiae argyi is to shield all single-root edible trees eaten by silkworms with a tent and close a window curtain of the tent.
Advantageous effects
Through long-term observation and research of the inventor, the inventor finds that the survival rate of the wild silkworm is difficult to improve in the prior art, the wild silkworm is suitable for the generation of germs under different environments due to the change of the natural climate and the temperature, so that the bacteria in the air circulation exist on animals and plants and are rapidly transmitted on the animals and plants suitable for the propagation of the germs. And the wild silkworm is a susceptible insect. The mode of culturing the wild silkworm at present is stocking in a mixed forest. There is no disinfection treatment for preventing diseases of wild silkworms in living environment and on the leaves (trees) eaten by the wild silkworms. Therefore, the cocoon forming rate of artificially cultured wild silkworms is low, about fifteen percent, and in most cases, the wild silkworms are covered by the whole army. Resulting in failure to form the amount of cocoon filaments required for the industry chain of the wild silkworm. Therefore, the field and industry of wild silkworms are from the hot stage to the cold stage from research to culture. The method stops the spread of infectious diseases by grasping the key link of sterilization and disinfection of the wild silkworm food, and better solves the problem of low survival rate of the wild silkworm. After years of cultivation experiments, good effects are obtained, and the cocooning rate of the giant silkworm is more than 65%.
The technical scheme of the invention is further illustrated by the following examples.
Detailed Description
The invention provides a sterilization and disinfection method in artificial culture of wild giant silkworm, which comprises the following steps:
and (3) foodstuff sterilization and disinfection: regularly sterilizing and disinfecting wild silkworm edible trees and edible tree leaves for feeding before the ants come out, firstly spraying potassium permanganate solution for sterilization and disinfection, and then spraying folium artemisiae argyi solution for sterilization and disinfection;
the regular sterilization and disinfection is to perform fixed-point and fixed-stump sterilization and disinfection on the silkworm feeding trees and the leaf feeding trees every two days five days before the ants come out;
the potassium permanganate solution is sprayed to sterilize, solid potassium permanganate and a water purification solution are prepared according to the proportion of 2-5: 1000-1800, and the potassium permanganate solution is sprayed to the root from the upper end of the main root of the single root edible tree;
the folium artemisiae argyi liquid is sprayed with the folium artemisiae argyi liquid for sterilization and disinfection, wherein the folium artemisiae argyi liquid is prepared by decocting dry folium artemisiae argyi and a water purification solution according to the proportion of 1: 10-20 to 10-13 kg with fire, filtering the folium artemisiae argyi after cooling, adding 150-240 kg of water, and spraying the upper layer liquid on the branch extending and the branch extending leaves of the single-stump edible tree for sterilization and disinfection;
sterilizing and disinfecting the outdoor environment: sterilizing and disinfecting the surrounding environment of the silkworm house by using a medicinal pasteurization solution and a potassium permanganate solution respectively before and after the emergence of the ants;
the sterilization and disinfection of the silkworm house peripheral environment by the medicinal pasteurization liquid and the potassium permanganate solution before and after the emergence of the ants respectively is to sterilize the silkworm house peripheral environment twice by the medicinal pasteurization liquid three days before the emergence of the ants, and sterilize the silkworm house peripheral environment by the potassium permanganate solution with the concentration of two thousandths to five thousandths of a day every 2 days after the emergence of the ants until the silkworm house peripheral environment is five-year old;
sterilizing and disinfecting wild silkworm indoor cultivation: closing the door and window before and after the ants come out, and smoking with dried folium Artemisiae Argyi;
the door and window is smoked by using the dry folium artemisiae argyi three days before the emergence of the ants, the door and window are closed by using the dry folium artemisiae argyi for 10 to 20 minutes every day, and the door and window are opened for ventilation one day before the emergence of the ants;
the door and window closing after the ants come out is smoked by using dry folium artemisiae argyi, the door and window are closed after the ants come out for 5 to 7 days, the folium artemisiae argyi is used for smoking, sterilizing and disinfecting for 10 to 20 minutes, then the door and window are opened for ventilation, the process is carried out until the silkworms enter into the third-instar stage and are moved to outdoor cultivation, and if 4-5-year-old silkworms are cultivated indoors until cocoons are formed, the operation is carried out according to the method;
the screen door and window are additionally arranged on the door and window to prevent insects such as parasitic wasps, aspongopus and the like from invading the door and window to infringe the tencel silkworms;
sterilizing and disinfecting wild silkworm outdoor cultivation: transferring young silkworms to the outdoor for culture after the young silkworms enter a third-instar period, and fumigating and sterilizing single-stump edible trees eaten by all silkworms regularly with folium artemisiae argyi; a circle of quicklime is scattered around the young silkworm on the periphery of the feeding single-stump feeding tree, so that the aim of sterilizing and disinfecting soil is fulfilled, and the invasion of centipede worms to the wild silkworm is prevented; the giant silkworm is covered by a nylon net for sensitization and prevention of the invasion of birds to the giant silkworm, sensitization can condition the physiological function of the giant silkworm, improve the immunity and spit out better silk (silky luster);
the regular folium artemisiae argyi fumigation and sterilization is to fumigate and sterilize folium artemisiae argyi for 10 to 20 minutes every 4 to 6 days;
the fumigation and sterilization with the folium artemisiae argyi is to shield all single-root edible trees eaten by silkworms with a tent, close a window curtain of the tent, and push the tent to one side for further use after the sterilization is finished;
the aperture of the mesh of the nylon net is 2.0 or 2.5 cm.
Claims (10)
1. A sterilization and disinfection method in artificial breeding of wild silkworm is characterized by comprising a food sterilization and disinfection step, wherein the food sterilization and disinfection step is to regularly sterilize and disinfect the bred wild silkworm edible tree and edible tree leaves before the ants emerge, and the sterilization and disinfection is carried out by spraying potassium permanganate solution and then spraying folium artemisiae argyi solution.
2. The method according to claim 1, wherein the regular sterilization is performed by performing fixed-point and fixed-stump sterilization on the natural silkworm feeding trees and the feeding leaves every two days five days before the ants come out.
3. The method as claimed in claim 1, wherein the potassium permanganate solution is sprayed to sterilize and disinfect the wild giant silkworm, the potassium permanganate solution and the purified water solution are mixed in a ratio of 2-5: 1000-1800, and the mixture is sprayed from the upper end of the main root of the single-root eating tree to the root.
4. The method as claimed in claim 1, wherein the folium artemisiae argyi liquid is sprayed to sterilize and disinfect the wild Bombyx mori by decocting the dry folium artemisiae argyi and the water purification solution in a ratio of 1: 10 to 20 to 10 to 13 kg, cooling, filtering the folium artemisiae argyi, adding 150 to 240 kg of water, and spraying the supernatant to the branches and leaves of the single-rhizome edible tree.
5. The method according to claim 1, further comprising an outdoor environment sterilization step of sterilizing the environment around the silkworm house with a medical pasteurization solution and a potassium permanganate solution before and after the emergence of the ants, respectively.
6. The method according to claim 5, wherein the sterilization and disinfection of the silkworm house environment with the medicinal pasteurization solution and the potassium permanganate solution before and after the emergence of the ants is carried out by sterilizing the silkworm house environment with the medicinal pasteurization solution twice three days before the emergence of the ants and sterilizing the silkworm house environment with the potassium permanganate solution with a concentration of two thousandths to five thousandths of a day every 2 days after the emergence of the ants until the silkworm house environment is five-year old.
7. The sterilization and disinfection method in artificial wild giant silkworm breeding according to claim 1, characterized by further comprising a sterilization and disinfection step of giant silkworm indoor breeding, wherein the sterilization and disinfection step of giant silkworm indoor breeding is to close a door and a window before and after the emergence of a termite and smoke with dried folium artemisiae argyi.
8. The method for sterilization and disinfection in artificial culture of wild giant silkworm according to claim 7, wherein the method comprises the following steps:
the door and window is smoked by using the dry folium artemisiae argyi three days before the emergence of the ants, the door and window are closed by using the dry folium artemisiae argyi for 10 to 20 minutes every day, and the door and window are opened for ventilation one day before the emergence of the ants;
the door and window is closed after the ants come out for 5 to 7 days, the door and window is closed after the ants come out, the folium artemisiae argyi is used for fumigation, sterilization and disinfection for 10 to 20 minutes, then the door and window are opened for ventilation, the method is carried out until the silkworms enter into the third-instar stage and are moved to outdoor cultivation, and if the silkworms of 4 to 5 instars are cultivated indoors until cocoons are formed, the method is also used for operation.
9. The method for sterilization and disinfection in artificial wild giant silkworm breeding according to claim 1, characterized by further comprising a step of sterilization and disinfection of giant silkworm outdoor breeding, wherein the step of sterilization and disinfection of giant silkworm outdoor breeding is that young silkworms are moved to outdoor breeding after entering a third-instar period, and all single-stump edible trees fed by silkworms are fumigated and disinfected with folium artemisiae argyi smoke at regular intervals; a circle of quicklime is scattered around the young silkworm on the periphery of the feeding single-stump feeding tree, so that the aim of sterilizing and disinfecting soil is fulfilled, and the invasion of centipede worms to the wild silkworm is prevented; the giant silkworm is covered by a nylon net for sensitization and prevention of the invasion of birds to the giant silkworm.
10. The method for sterilization and disinfection in artificial culture of wild giant silkworm according to claim 9, wherein the periodical folium artemisiae argyi fumigation and disinfection is performed for 10 to 20 minutes every 4 to 6 days; the fumigation and sterilization with the folium artemisiae argyi is to shield all single-root edible trees eaten by silkworms with a tent and close a window curtain of the tent.
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Application publication date: 20201023 |