CN111802201A - Pleione bud cutting propagation method - Google Patents
Pleione bud cutting propagation method Download PDFInfo
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- CN111802201A CN111802201A CN202010511560.9A CN202010511560A CN111802201A CN 111802201 A CN111802201 A CN 111802201A CN 202010511560 A CN202010511560 A CN 202010511560A CN 111802201 A CN111802201 A CN 111802201A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/35—Bulbs; Alliums, e.g. onions or leeks
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C21/00—Methods of fertilising, sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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Abstract
The invention discloses a Pleione bud cutting propagation method, which comprises the following operation steps: and (3) pseudobulb germination acceleration: transplanting the collected Pleione pseudobulb into a greenhouse with rich humus content, sufficient base fertilizer and loose and breathable soil, and keeping certain indoor temperature and humidity; preparing a seedbed: breaking bamboo rods into pieces, manufacturing a seedbed in a greenhouse by using the bamboo pieces, scattering a layer of lime at the bottom of the seedbed, proportioning leaf mold, coconut chaff and burnt lime, adding fully-decomposed cow dung, uniformly stirring, laying a matrix on the seedbed, raking, filling drip irrigation, and thoroughly watering the mixed matrix by using a spraying method. Compared with other treatment processes, the method has the advantages that the main buds of the pleione bulbocodioides are cut off and subjected to secondary pregermination, various special treatments are carried out in the processes of main bud cutting, secondary pregermination and sprout transplanting and managing, the temperature and the humidity are reasonably controlled, the ventilation of a greenhouse is kept, an excellent environment is provided for the growth and the growth of the sprouts, a large number of seedlings are obtained, and the pseudobulb propagation coefficient is improved.
Description
Technical Field
The invention relates to the technical field of artificial planting of medicinal plants, in particular to a Pleione bulbocodioides bud cutting propagation method.
Background
The Pleione is a national secondary protection plant of Pleione of Orchidaceae, and is also a unique species of China, and is mainly distributed in southwest of China, and uses pseudobulb as medicine, and is known as Pseudobulbus Cremastrae Seu pleiones, and has effects of clearing heat, removing toxic substances, eliminating phlegm and resolving masses, and in recent years, due to its anti-tumor effect, it is concerned about, wild resources are severely exhausted, and prices are rising.
The existing Pleione propagation method has certain defects: the artificial cultivation cannot be promoted due to the difficulty in seedling production, the Pleione is an orchid plant, seeds have no endosperm and are difficult to germinate and grow in a natural state, the emergence rate is low in the conventional method, a large number of seedlings are difficult to obtain, one Pleione can only generate a new pseudobulb every year, and the propagation coefficient of the traditional plant division method is extremely low.
Disclosure of Invention
The invention aims to solve the defects in the prior art, and provides a Pleione rhizome cutting propagation method which comprises the steps of cutting off main buds of Pleione rhizome, accelerating germination for the second time, spraying plant ash containing carbendazim in the processes of cutting off the main buds, accelerating germination for the second time, transplanting new buds and managing and protecting the new buds to prevent cuts from decaying, removing the new buds with weak growth vigor in the process of culturing the new buds to enable the new buds with good growth vigor to be easier to produce, spraying foliar fertilizer to promote the new buds to develop and take root, coating tips of pseudo-bulb stems with 6-BA in the process of accelerating germination for the second time to promote the new buds to germinate for the second time, reasonably controlling the temperature and humidity, keeping the ventilation of a greenhouse, providing an excellent environment for the development and growth of the new buds, obtaining a large number of seedlings in different plants, and improving the propagation coefficient of the pseudo-bulb stems.
In order to achieve the purpose, the invention provides the following technical scheme: a method for cutting buds of Pleione bulbocodioides comprises the following steps:
s1, pseudobulb germination acceleration: transplanting the collected Pleione pseudobulb into a greenhouse with rich humus content, sufficient base fertilizer and loose and breathable soil, and keeping certain indoor temperature and humidity;
s2, preparing a seedbed: breaking bamboo rods, manufacturing a seedbed in a greenhouse by using the bamboo rods, scattering a layer of lime at the bottom of the seedbed, proportioning leaf mold, coconut coir and soil ash, adding fully decomposed cow dung, uniformly stirring, laying a matrix on the seedbed, raking, filling drip irrigation, and thoroughly watering the mixed matrix by using a spraying method;
s3, main bud excision: cutting off the main buds when the pseudobulbs at the base parts of the main buds expand to a certain diameter and the number of the pseudobulbs is 5-7, placing the main buds in a shade place for half a day, treating the cut with plant ash mixed with carbendazim after the cut is slightly dried, and transplanting the main buds to a seedbed;
s4, secondary germination accelerating: spraying pseudobulb with main bud removed with carbendazim to prevent cut from rotting, maintaining indoor temperature and humidity, removing petiole residue at tip of pseudobulb, and dipping 6-BA with cotton swab to smear tip of pseudobulb;
s5, sprout culture: about 1 month later, 2-8 new buds can sprout at the tip of the pseudobulb of the pleione bulbocodioides, if more new buds exist, the buds with weak growth vigor are removed, leaf fertilizer is sprayed after leaves of the new buds are slightly unfolded, a solution prepared by rooting powder is sprayed on the new buds after about 10 days later, and meanwhile, a greenhouse door is opened for ventilation, so that the indoor temperature is reduced;
s6, sprout excision: after 2 months, the pseudobulb at the base part of the sprout expands to a certain diameter, when the number of the root is 5-7, the sprout is carefully cut off one by one, the new root which germinates is strictly prevented from being damaged in the cutting process, and the cut is slightly dried after the sprout is cut off and placed in a shade for airing for half a day;
s7, transplanting new buds and managing and protecting: the incision is handled with the plant ash that thoughtlessly has carbendazim with the sprout that the incision is slightly dry, later with sprout transplanting to the seedbed, the matrix covers root and false bulb simultaneously during the transplantation, opens the sprinkling irrigation facility, the greenhouse is covered the cloudy net outward, makes the cloudy net of hiding have certain printing opacity effect, opens the greenhouse both ends and carries out ventilation, the field planting after the completion can.
Preferably, the indoor temperature maintained in the pseudobulb germination accelerating step and the secondary germination accelerating step is 18-35 ℃ and the humidity is not lower than 60%.
Preferably, the size of the seedbed in the seedbed preparation step is 10m in length, 0.9m in width and 0.1m in height.
Preferably, the ratio of leaf mold, leaf bran and fire soil in the seedbed preparation step is 2:2:1, and the content of cow dung is 5%.
Preferably, the pseudobulb expansion diameter at the base of the main bud in the main bud excision step is 0.4cm-0.6 cm.
Preferably, the pseudobulb expansion diameter at the base of the sprout in the sprout excision step is 0.4cm-0.6 cm.
Preferably, the content of carbendazim in plant ash used in the main bud excision step, the secondary germination accelerating step and the new bud transplanting and managing step is 0.5%.
Preferably, the concentration of the 6-BA smeared at the tip of the pseudobulb in the secondary germination accelerating step is 1mg/L-2 mg/L.
Preferably, the light transmittance of the shading net in the step of transplanting the new buds and managing and protecting is 40% -60%.
The invention has the technical effects and advantages that:
according to the invention, main buds of the Pleione bulbocodioides are cut off and subjected to secondary pregermination, plant ash containing carbendazim is used for spraying in the processes of main bud cutting, secondary pregermination and sprout transplanting and managing, so that cut decay is prevented, sprouts with weak growth vigor are removed in the sprout culture process, so that sprouts with good growth vigor are easier to produce, in addition, foliar fertilizer is sprayed to promote sprout development and rooting, in the secondary pregermination process, the tip of a pseudobulb is coated with 6-BA to promote secondary sprouting, the temperature and humidity are reasonably controlled, the ventilation of a greenhouse is maintained, an excellent environment is provided for the development and growth of the sprouts, a large number of seedling branches are obtained, and the propagation coefficient of the pseudobulb is improved.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
A method for cutting buds of Pleione bulbocodioides comprises the following steps:
s1, pseudobulb germination acceleration: transplanting the collected Pleione pseudobulb into a greenhouse with rich humus content, sufficient base fertilizer and loose and breathable soil, and keeping certain indoor temperature and humidity;
s2, preparing a seedbed: breaking bamboo rods, manufacturing a seedbed in a greenhouse by using the bamboo rods, scattering a layer of lime at the bottom of the seedbed, proportioning leaf mold, coconut coir and soil ash, adding fully decomposed cow dung, uniformly stirring, laying a matrix on the seedbed, raking, filling drip irrigation, and thoroughly watering the mixed matrix by using a spraying method;
s3, main bud excision: cutting off the main buds when the pseudobulbs at the base parts of the main buds expand to a certain diameter and the number of the pseudobulbs is 5-7, placing the main buds in a shade place for half a day, treating the cut with plant ash mixed with carbendazim after the cut is slightly dried, and transplanting the main buds to a seedbed;
s4, secondary germination accelerating: spraying pseudobulb with main bud removed with carbendazim to prevent cut from rotting, maintaining indoor temperature and humidity, removing petiole residue at tip of pseudobulb, and dipping 6-BA with cotton swab to smear tip of pseudobulb;
s5, sprout culture: about 1 month later, 2-8 new buds can sprout at the tip of the pseudobulb of the pleione bulbocodioides, if more new buds exist, the buds with weak growth vigor are removed, leaf fertilizer is sprayed after leaves of the new buds are slightly unfolded, a solution prepared by rooting powder is sprayed on the new buds after about 10 days later, and meanwhile, a greenhouse door is opened for ventilation, so that the indoor temperature is reduced;
s6, sprout excision: after 2 months, the pseudobulb at the base part of the sprout expands to a certain diameter, when the number of the root is 5-7, the sprout is carefully cut off one by one, the new root which germinates is strictly prevented from being damaged in the cutting process, and the cut is slightly dried after the sprout is cut off and placed in a shade for airing for half a day;
s7, transplanting new buds and managing and protecting: the incision is handled with the plant ash that thoughtlessly has carbendazim with the sprout that the incision is slightly dry, later with sprout transplanting to the seedbed, the matrix covers root and false bulb simultaneously during the transplantation, opens the sprinkling irrigation facility, the greenhouse is covered the cloudy net outward, makes the cloudy net of hiding have certain printing opacity effect, opens the greenhouse both ends and carries out ventilation, the field planting after the completion can.
Preferably, the room temperature maintained in the pseudobulb germination accelerating step and the secondary germination accelerating step is 18 to 35 ℃ and the humidity is not less than 60%.
Preferably, the size of the seedbed in the seedbed preparation step is 10m in length, 0.9m in width and 0.1m in height.
Preferably, the ratio of leaf mold, leaf bran and fire soil in the seedbed preparation step is 2:2:1, and the content of cow dung is 5%.
Preferably, the pseudobulb expansion diameter at the base of the main bud in the main bud excision step is 0.5 cm.
Preferably, the pseudobulb expansion diameter at the base of the sprout in the sprout excision step is 0.5 cm.
Preferably, the content of carbendazim in plant ash used in the main bud excision step, the secondary germination accelerating step and the new bud transplanting and managing step is 0.5%.
Preferably, the concentration of 6-BA applied to the tip of the pseudobulb in the secondary pregermination step is 1 mg/L.
Preferably, the light transmittance of the shading net in the step of transplanting the new buds and protecting the new buds is 50%.
The number of the Pleione sprouts cultivated by the above process is: each single-clove orchid can propagate 10 new seedlings, and the culture survival rate of the new single-clove orchid plants is greatly improved.
Example 2
A method for cutting buds of Pleione bulbocodioides comprises the following steps:
s1, pseudobulb germination acceleration: transplanting the collected Pleione pseudobulb into a greenhouse with rich humus content, sufficient base fertilizer and loose and breathable soil, and keeping certain indoor temperature and humidity;
s2, preparing a seedbed: breaking bamboo rods, manufacturing a seedbed in a greenhouse by using the bamboo rods, scattering a layer of lime at the bottom of the seedbed, proportioning leaf mold, coconut coir and soil ash, adding fully decomposed cow dung, uniformly stirring, laying a matrix on the seedbed, raking, filling drip irrigation, and thoroughly watering the mixed matrix by using a spraying method;
s3, main bud excision: cutting off the main buds when the pseudobulbs at the base parts of the main buds expand to a certain diameter and the number of the pseudobulbs is 5-7, placing the main buds in a shade place for half a day, treating the cut with plant ash mixed with carbendazim after the cut is slightly dried, and transplanting the main buds to a seedbed;
s4, secondary germination accelerating: spraying pseudobulb with main bud removed with carbendazim to prevent cut from rotting, maintaining indoor temperature and humidity, removing petiole residue at tip of pseudobulb, and dipping 6-BA with cotton swab to smear tip of pseudobulb;
s5, sprout culture: about 1 month later, 2-8 new buds can sprout at the tip of the pseudobulb of the pleione bulbocodioides, if more new buds exist, the buds with weak growth vigor are removed, leaf fertilizer is sprayed after leaves of the new buds are slightly unfolded, a solution prepared by rooting powder is sprayed on the new buds after about 10 days later, and meanwhile, a greenhouse door is opened for ventilation, so that the indoor temperature is reduced;
s6, sprout excision: after 2 months, the pseudobulb at the base part of the sprout expands to a certain diameter, when the number of the root is 5-7, the sprout is carefully cut off one by one, the new root which germinates is strictly prevented from being damaged in the cutting process, and the cut is slightly dried after the sprout is cut off and placed in a shade for airing for half a day;
s7, transplanting new buds and managing and protecting: the incision is handled with the plant ash that thoughtlessly has carbendazim with the sprout that the incision is slightly dry, later with sprout transplanting to the seedbed, the matrix covers root and false bulb simultaneously during the transplantation, opens the sprinkling irrigation facility, the greenhouse is covered the cloudy net outward, makes the cloudy net of hiding have certain printing opacity effect, opens the greenhouse both ends and carries out ventilation, the field planting after the completion can.
Preferably, the room temperature maintained in the pseudobulb germination accelerating step and the secondary germination accelerating step is 18 to 35 ℃ and the humidity is not less than 60%.
Preferably, the size of the seedbed in the seedbed preparation step is 10m in length, 0.9m in width and 0.1m in height.
Preferably, the ratio of leaf mold, leaf bran and fire soil in the seedbed preparation step is 2:2:1, and the content of cow dung is 5%.
Preferably, the pseudobulb expansion diameter at the base of the main bud in the main bud excision step is 0.4 cm.
Preferably, the pseudobulb expansion diameter at the base of the sprout in the sprout excision step is 0.4 cm.
Preferably, the content of carbendazim in plant ash used in the main bud excision step, the secondary germination accelerating step and the new bud transplanting and managing step is 0.5%.
Preferably, the concentration of 6-BA applied to the tip of the pseudobulb in the secondary pregermination step is 1.5 mg/L.
Preferably, the light transmittance of the shading net in the step of transplanting the new buds and protecting the new buds is 40%.
The number of the Pleione sprouts cultivated by the above process is: each single-clove orchid can propagate 8 new seedlings, and the culture survival rate of the new single-clove orchid plants is greatly improved.
Example 3
A method for cutting buds of Pleione bulbocodioides comprises the following steps:
s1, pseudobulb germination acceleration: transplanting the collected Pleione pseudobulb into a greenhouse with rich humus content, sufficient base fertilizer and loose and breathable soil, and keeping certain indoor temperature and humidity;
s2, preparing a seedbed: breaking bamboo rods, manufacturing a seedbed in a greenhouse by using the bamboo rods, scattering a layer of lime at the bottom of the seedbed, proportioning leaf mold, coconut coir and soil ash, adding fully decomposed cow dung, uniformly stirring, laying a matrix on the seedbed, raking, filling drip irrigation, and thoroughly watering the mixed matrix by using a spraying method;
s3, main bud excision: cutting off the main buds when the pseudobulbs at the base parts of the main buds expand to a certain diameter and the number of the pseudobulbs is 5-7, placing the main buds in a shade place for half a day, treating the cut with plant ash mixed with carbendazim after the cut is slightly dried, and transplanting the main buds to a seedbed;
s4, secondary germination accelerating: spraying pseudobulb with main bud removed with carbendazim to prevent cut from rotting, maintaining indoor temperature and humidity, removing petiole residue at tip of pseudobulb, and dipping 6-BA with cotton swab to smear tip of pseudobulb;
s5, sprout culture: about 1 month later, 2-8 new buds can sprout at the tip of the pseudobulb of the pleione bulbocodioides, if more new buds exist, the buds with weak growth vigor are removed, leaf fertilizer is sprayed after leaves of the new buds are slightly unfolded, a solution prepared by rooting powder is sprayed on the new buds after about 10 days later, and meanwhile, a greenhouse door is opened for ventilation, so that the indoor temperature is reduced;
s6, sprout excision: after 2 months, the pseudobulb at the base part of the sprout expands to a certain diameter, when the number of the root is 5-7, the sprout is carefully cut off one by one, the new root which germinates is strictly prevented from being damaged in the cutting process, and the cut is slightly dried after the sprout is cut off and placed in a shade for airing for half a day;
s7, transplanting new buds and managing and protecting: the incision is handled with the plant ash that thoughtlessly has carbendazim with the sprout that the incision is slightly dry, later with sprout transplanting to the seedbed, the matrix covers root and false bulb simultaneously during the transplantation, opens the sprinkling irrigation facility, the greenhouse is covered the cloudy net outward, makes the cloudy net of hiding have certain printing opacity effect, opens the greenhouse both ends and carries out ventilation, the field planting after the completion can.
Preferably, the room temperature maintained in the pseudobulb germination accelerating step and the secondary germination accelerating step is 18 to 35 ℃ and the humidity is not less than 60%.
Preferably, the size of the seedbed in the seedbed preparation step is 10m in length, 0.9m in width and 0.1m in height.
Preferably, the ratio of leaf mold, leaf bran and fire soil in the seedbed preparation step is 2:2:1, and the content of cow dung is 5%.
Preferably, the pseudobulb expansion diameter at the base of the main bud in the main bud excision step is 0.6 cm.
Preferably, the pseudobulb expansion diameter at the base of the sprout in the sprout excision step is 0.6 cm.
Preferably, the content of carbendazim in plant ash used in the main bud excision step, the secondary germination accelerating step and the new bud transplanting and managing step is 0.5%.
Preferably, the concentration of 6-BA applied to the tip of the pseudobulb in the secondary pregermination step is 2 mg/L.
Preferably, the light transmittance of the shading net in the step of transplanting the new buds and protecting the new buds is 60 percent.
The number of the Pleione sprouts cultivated by the above process is: each single-clove orchid can propagate 13 new seedlings, and the culture survival rate of the new single-clove orchid plants is greatly improved.
To sum up: compared with other treatment processes, the Pleione cutting bud propagation method provided by the invention has the following advantages: by cutting off main buds of the Pleione odorata and carrying out secondary pregermination, plant ash containing carbendazim is used for spraying in the processes of main bud cutting, secondary pregermination and sprout transplanting and managing, so that the cut is prevented from rotting, sprouts with weak growth vigor are removed in the sprout culture process, the sprouts with good growth vigor are easier to produce, foliar fertilizer is sprayed to promote sprout development and rooting, the tip of the pseudobulb is smeared by using 6-BA in the secondary pregermination process to promote secondary sprouting, the temperature and humidity are reasonably controlled, the ventilation of a greenhouse is kept, an excellent environment is provided for the development and growth of the sprouts, a large number of plant-dividing seedlings are obtained, and the propagation coefficient of the pseudobulb is improved.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments or portions thereof without departing from the spirit and scope of the invention.
Claims (9)
1. A Pleione bud cutting propagation method is characterized in that: the method comprises the following operation steps:
s1, pseudobulb germination acceleration: transplanting the collected Pleione pseudobulb into a greenhouse with rich humus content, sufficient base fertilizer and loose and breathable soil, and keeping certain indoor temperature and humidity;
s2, preparing a seedbed: breaking bamboo rods, manufacturing a seedbed in a greenhouse by using the bamboo rods, scattering a layer of lime at the bottom of the seedbed, proportioning leaf mold, coconut coir and soil ash, adding fully decomposed cow dung, uniformly stirring, laying a matrix on the seedbed, raking, filling drip irrigation, and thoroughly watering the mixed matrix by using a spraying method;
s3, main bud excision: cutting off the main buds when the pseudobulbs at the base parts of the main buds expand to a certain diameter and the number of the pseudobulbs is 5-7, placing the main buds in a shade place for half a day, treating the cut with plant ash mixed with carbendazim after the cut is slightly dried, and transplanting the main buds to a seedbed;
s4, secondary germination accelerating: spraying pseudobulb with main bud removed with carbendazim to prevent cut from rotting, maintaining indoor temperature and humidity, removing petiole residue at tip of pseudobulb, and dipping 6-BA with cotton swab to smear tip of pseudobulb;
s5, sprout culture: about 1 month later, 2-8 new buds can sprout at the tip of the pseudobulb of the pleione bulbocodioides, if more new buds exist, the buds with weak growth vigor are removed, leaf fertilizer is sprayed after leaves of the new buds are slightly unfolded, a solution prepared by rooting powder is sprayed on the new buds after about 10 days later, and meanwhile, a greenhouse door is opened for ventilation, so that the indoor temperature is reduced;
s6, sprout excision: after 2 months, the pseudobulb at the base part of the sprout expands to a certain diameter, when the number of the root is 5-7, the sprout is carefully cut off one by one, the new root which germinates is strictly prevented from being damaged in the cutting process, and the cut is slightly dried after the sprout is cut off and placed in a shade for airing for half a day;
s7, transplanting new buds and managing and protecting: the incision is handled with the plant ash that thoughtlessly has carbendazim with the sprout that the incision is slightly dry, later with sprout transplanting to the seedbed, the matrix covers root and false bulb simultaneously during the transplantation, opens the sprinkling irrigation facility, the greenhouse is covered the cloudy net outward, makes the cloudy net of hiding have certain printing opacity effect, opens the greenhouse both ends and carries out ventilation, the field planting after the completion can.
2. The Pleione bulbocodioides cutting propagation method according to claim 1, wherein: the indoor temperature in the pseudobulb germination accelerating step and the indoor temperature in the secondary germination accelerating step are kept at 18-35 ℃, and the humidity is not lower than 60%.
3. The Pleione bulbocodioides cutting propagation method according to claim 1, wherein: the size of the seedbed in the seedbed preparation step is 10m in length, 0.9m in width and 0.1m in height.
4. The Pleione bulbocodioides cutting propagation method according to claim 1, wherein: in the step of preparing the seedbed, the proportion of leaf mold, leaf chaff and fire soil is 2:2:1, and the content of cow dung is 5%.
5. The Pleione bulbocodioides cutting propagation method according to claim 1, wherein: and in the step of cutting the main bud, the expansion diameter of pseudobulb at the base part of the main bud is 0.4cm-0.6 cm.
6. The Pleione bulbocodioides cutting propagation method according to claim 1, wherein: and in the step of cutting the sprout, the expansion diameter of pseudobulb at the base part of the sprout is 0.4cm-0.6 cm.
7. The Pleione bulbocodioides cutting propagation method according to claim 1, wherein: the content of carbendazim in plant ash used in the main bud excision step, the secondary germination accelerating step and the new bud transplanting and managing step is 0.5 percent.
8. The Pleione bulbocodioides cutting propagation method according to claim 1, wherein: the concentration of 6-BA smeared at the tip of the pseudobulb in the secondary germination accelerating step is 1mg/L-2 mg/L.
9. The Pleione bulbocodioides cutting propagation method according to claim 1, wherein: the light transmittance of the shading net in the step of transplanting the new buds and protecting the new buds is 40-60%.
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