CN111795553B - Anti-pollution dehydration auxiliary device and application method thereof - Google Patents
Anti-pollution dehydration auxiliary device and application method thereof Download PDFInfo
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- CN111795553B CN111795553B CN202010690525.8A CN202010690525A CN111795553B CN 111795553 B CN111795553 B CN 111795553B CN 202010690525 A CN202010690525 A CN 202010690525A CN 111795553 B CN111795553 B CN 111795553B
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- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F26—DRYING
- F26B—DRYING SOLID MATERIALS OR OBJECTS BY REMOVING LIQUID THEREFROM
- F26B9/00—Machines or apparatus for drying solid materials or objects at rest or with only local agitation; Domestic airing cupboards
- F26B9/06—Machines or apparatus for drying solid materials or objects at rest or with only local agitation; Domestic airing cupboards in stationary drums or chambers
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- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F26—DRYING
- F26B—DRYING SOLID MATERIALS OR OBJECTS BY REMOVING LIQUID THEREFROM
- F26B21/00—Arrangements or duct systems, e.g. in combination with pallet boxes, for supplying and controlling air or gases for drying solid materials or objects
- F26B21/003—Supply-air or gas filters
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Abstract
The invention relates to a plant pathology technology, and particularly discloses an anti-pollution dehydration auxiliary device and an application method thereof. The auxiliary device mainly comprises components of an airflow engine A1, an airflow connecting pipe A2, an airflow distribution and pressure regulation box A3, an air guide pipe A4, an airflow spray head A5, an airflow injector fixing plate A6, an airflow injector base A7, a sample bedplate B1 and a sample bedplate base pad B2. The auxiliary device needs to be matched with a super clean bench for use, and the application method mainly comprises the following steps: 1) starting and operating the super clean bench; 2) assembling an auxiliary device; 3) sterile purification of the auxiliary device; 4) placing a sample material; 5) running of dehydration work; 6) and finishing the work. The invention has the advantages that: 1) expensive equipment conditions are not required; 2) the technical scheme is simple and easy to implement; 3) the reliability of maintaining the biological activity of the sample material is high; 4) can effectively prevent biological pollution.
Description
Technical Field
The invention relates to a plant pathology technology, in particular to an auxiliary device for quickly dehydrating plant pathology research materials under the conditions of sterility and normal temperature and an application method thereof.
Technical Field
Effective control of plant diseases relies on fundamental research on plant pathogens, requiring extensive exploration and understanding of pathogen biology. In the research and exploration process, some pathogenic bacteria materials or some biological materials related to the research and exploration need to be dehydrated for rapid drying or concentration, and the dehydration comprises the steps of suspending and dispersing pathogenic bacteria (such as rice bacterial blight) strain materials or biological control strain materials in a protective agent and performing dry preservation; drying and storing pure spore suspension obtained by culturing some pathogenic fungi (such as Magnaporthe grisea); drying and storing some pathogenic fungi (such as Rhizoctonia solani) sclerotium material; the mycelium growing on the plant seed material is dried and stored (such as rice blast), and the separation exploration products or intermediate products of some biological source control medicines are concentrated or dried, and the like. The biological materials are characterized in that the biological materials cannot be polluted and need to keep biological activity, so that the rapid dehydration of the materials cannot be carried out under conventional conditions by adopting a sun-drying, blow-drying or baking technology, the currently feasible and effective dehydration technology is mainly a freeze-drying technology, however, the freeze-drying technology easily causes cell ice crystal phenomena, so that the biological activity is influenced, and expensive freeze-drying machine conditions are lacked in many common laboratories, so that the research work related to the development of some common laboratories is influenced or limited.
Disclosure of Invention
The invention aims to provide an auxiliary device for quickly dehydrating a plant pathology research material under the sterile and normal temperature conditions and an application method thereof.
The invention adopts an auxiliary device for anti-pollution dehydration to be matched with a super clean bench (hereinafter referred to as a super clean bench) for use, solves the technical problems, and adopts the following technical scheme:
1. an auxiliary device for anti-pollution dehydration mainly comprises an airflow ejector A and a sample table B.
The air flow ejector A part comprises a component air flow engine A1, an air flow connecting pipe A2, an air flow distribution and pressure regulation box A3, an air duct A4, an air flow spray head A5, an air flow ejector fixing plate A6 and an air flow ejector base A7. The component airflow distribution and pressure regulation box A3 is provided with an airflow distribution structure and an air pressure regulation structure.
Six components of an airflow engine A1, an airflow connecting pipe A2, an airflow distribution and pressure regulation box A3, an air duct A4, an airflow spray head A5 and an injector fixing plate A6 are firmly connected together to form an airflow injector assembly.
Sample stage B components, including component sample stage B1, sample stage base pad B2.
2. An application method of an auxiliary device for anti-pollution dehydration is disclosed, the auxiliary device is matched with a super clean bench for use, and the application method comprises the following steps:
1) starting and operating the super clean bench: the clean bench is cleaned normally according to the routine procedure, then the clean bench is started to run and the sterile air flow rate is adjusted to be used normally.
2) Assembling the auxiliary device: the auxiliary device is assembled in the running state of the super clean bench, and the assembling operation is as follows:
firstly, transferring the components into a super clean bench: taking out the components of the auxiliary device from the storage container and transferring the components into a super clean bench; the power supply lead of the airflow actuator a1 is pulled out of the clean bench.
Purifying the surface of the component: in the running super clean bench, the surfaces of the components of the auxiliary device are cleaned in a flapping vibration mode.
Third, the special purification of the airflow spray head A5: the outer surface of the air jet a5 was specially decontaminated with 75% alcohol and sterile moistened gauze.
Assembling the components: firstly, the base A7 of the airflow jet is placed on a working table, the airflow jet assembly is placed on the base A7 of the airflow jet assembly, the sample base pad B2 is placed below the airflow jet head A5 of the assembly, and then the sample table plate B1 is placed on the sample base pad B2.
3) Sterile purification of the auxiliary device: and 2) after the operation is finished, performing sterile purification on the auxiliary device according to the following operations:
firstly, continuously operating the super clean bench for more than 5 minutes;
starting a running airflow engine A1; sterile air is drawn into the inner pipe of the airflow sprayer and is sprayed out from the airflow nozzle A5;
and knocking each component through which the sterile airflow passes in the auxiliary device to promote the purification of the internal airway.
And fourthly, the ultra-clean bench and the airflow engine A1 are kept running for more than 10 minutes, and the sterile purification of the internal air passage of the auxiliary device is ensured.
4) Placing of sample material: after the operation of the step 3), placing a sample material according to the following operation:
firstly, transferring a sample material to be concentrated and dried into a proper sample bottle in advance;
secondly, opening the bottle cap of the sample bottle in the first operation, placing the uncapped sample bottle on the surface of the sample bedplate B1, and moving the sample bottle to a position right below the airflow nozzle A5;
thirdly, the sample bottle is lifted, and the airflow nozzle A5 is inserted into the sample bottle and is close to the liquid level of the sample in the bottle;
adjusting the sterile airflow sprayed by the airflow nozzle A5 to a proper intensity;
5) and (3) operation of dehydration: keeping the working state of the step 4), the sample material is continuously sprayed by the sterile air flow sprayed by the air flow nozzle A5 of the auxiliary device, so that the sample material is quickly dehydrated, concentrated and dried.
6) And (5) finishing the work: and 5) after the dehydration is finished, taking out the sample bottle, and obtaining a dehydrated sample material after the cover is returned. Shutting down the airflow initiator a 1; the components of the auxiliary device which can be freely disassembled are unloaded one by one and are transferred back to a clean container for storage; and finally, shutting down the clean bench.
The invention has the advantages that:
1) no expensive equipment conditions are required. 2) The technical scheme is simple and easy to implement, and can be implemented in a common laboratory. 3) Can be dehydrated under the condition of normal temperature, and pathogenic bacteria are not easy to inactivate, so that the reliability of maintaining the biological activity of the sample material is high. 4) The sample material is in a sterile environment in the whole process, and biological pollution can be effectively prevented.
Drawings
Fig. 1 is a side view schematically showing the structure of the main part of the auxiliary device of the present invention. In the figure, an air flow ejector A and a sample stage B are arranged.
Fig. 2 is a schematic structural view of the auxiliary device of the present invention. In the figure, an airflow engine A1, an airflow connecting pipe A2, an airflow distribution and pressure regulation box A3, an air duct A4, an airflow spray head A5, an airflow injector fixing plate A6, an airflow injector base A7 and an air duct adaptive small hole A8; sample platen B1, sample platen base pad B2. 2-1 is a schematic side view and 2-2 is a schematic front view.
FIG. 3 is a schematic view of the auxiliary device of the present invention after a sample is placed thereon. In the figure, an airflow engine A1, an airflow connecting pipe A2, an airflow distribution and pressure regulation box A3, an air duct A4, an airflow spray head A5, an airflow injector fixing plate A6, an airflow injector base A7 and an air duct adaptive small hole A8; sample platen B1, sample platen bottom pad B2; sample bottle C1, sample liquid C2. 3-1 is a schematic side view and 3-2 is a schematic front view.
Detailed description of the preferred embodiments
The invention is further described below with reference to the figures and examples.
The invention relates to an auxiliary device for anti-pollution dehydration and an application method thereof, wherein the auxiliary device is matched with a super clean bench for use, the whole function and the purpose are that the auxiliary device converts the sterile air drive of the super clean bench into jet air flow with higher flowing speed, and the jet air flow is adopted to jet a sample material, so that the sample material is quickly evaporated and dehydrated, and the purpose of quickly concentrating or drying the sample under the sterile condition and the normal temperature condition is achieved.
The auxiliary device is shown in figure 1, and mainly comprises two parts, namely a gas flow ejector A and a sample stage B.
The air flow ejector A component comprises a component air flow engine A1, an air flow connecting pipe A2, an air flow distribution and pressure regulation box A3, an air duct A4, an air flow spray head A5, an ejector fixing plate A6 and an ejector base A7, as shown in figure 2.
The air flow actuator A1 and the air flow distribution and pressure regulation box A3 are fixed on the ejector fixing plate A6 by common adhesive tapes, so that the movement of components caused by micro-vibration generated by the operation of the air flow actuator A1 is avoided. Therefore, six components, namely the airflow actuator A1, the airflow connecting pipe A2, the airflow distribution and pressure regulation box A3, the air guide pipe A4, the airflow nozzle A5 and the injector fixing plate A6, are firmly connected together to form an airflow injector assembly.
The airflow actuator a1 is an electric appliance that drives air flow using electric power, and uses an external power source.
The airflow connecting pipe A2 and the air duct A4 both adopt hoses with smooth surfaces.
The air flow distribution and pressure regulation box A3 is made of a common plastic box with a smooth surface, a plurality of small holes A8 are drilled in the plastic box, the size of the hole is matched with that of the air guide tube A4, single air flow provided by the air flow engine A1 is divided into a plurality of air flows by the box, and part of the small holes are used as air release holes and are plugged by clean rubber plugs to play a role in regulating the air pressure in the box; the intensity of the airflow emitted from airflow nozzle A5 is controlled by adjusting the air pressure in the box.
The airflow nozzle A5 is a small tubular nozzle with smooth surface, is made of a pipette tip, and a tip nozzle of the pipette tip is cut off as required to increase the caliber of the nozzle properly.
The ejector fixing plate A6 is a flat plate with a smooth surface and is made of a5 mm-thick organic glass plate, and small holes are drilled in the flat plate, are matched with the air flow nozzle A5 and can clamp and stabilize the air flow nozzle A5.
Injector mount a7 is a square piece that acts as a stable carrier for the manifold of the jet ejector, has a smooth surface, is free to attach to and detach from the manifold.
Sample station B includes sample platen B1 and sample station bottom pad B2, as shown in fig. 2.
The sample platen B1, which serves to carry the sample material, was made of a smooth surfaced plastic sheet.
The sample table bottom pad B2 is a square object with a smooth surface, the height of the element can be freely changed according to the size of a sample bottle, and the element is not fixedly connected with the sample table plate B1.
The sample stage B is not fixedly connected with the airflow ejector A.
The auxiliary device has no fixedly connected components, can be freely disassembled and assembled, and can be stored in a clean container (such as a plastic bag, a paper box and the like) for standby use in a non-use state.
The auxiliary device of the invention needs to be matched with a super clean bench for application, and the application method comprises the following steps:
the clean bench is prepared. And (4) normally cleaning the super clean bench according to a conventional program, then starting up the super clean bench to operate the super clean bench, and adjusting the flow of the sterile air of the super clean bench to the flow which is normally used.
The auxiliary device is then assembled and operated according to the following program:
keeping the running state of the super clean bench, lifting the glass baffle of the super clean bench, taking out each component of the auxiliary device from the storage container, transferring the components to the working surface of the super clean bench, jacking the glass baffle of the super clean bench by a small object to form a small gap, and pulling the power supply lead of the air flow ejector A out of the super clean bench from the gap. Then, each component of the auxiliary device is lightly knocked by using a clean hard object (such as scissors, tweezers and the like), particles or microorganisms which are attached to the surface of the component and have weak adhesive force can be vibrated to be separated from the surface, and the particles or microorganisms are taken away along with flowing sterile airflow, so that the purpose of purifying the surface is achieved.
The gas flow nozzle a5 is a key component for the auxiliary device of the present invention to function, and requires special cleaning treatment. The outer surface of the air flow nozzle A5 can be sterilized by 75% alcohol, and then the outer surface of the air flow nozzle A5 is wiped clean by using pre-prepared sterilized moist gauze.
Then, the components are assembled into a working auxiliary device, and the operation steps are as follows: the jet ejector base a7 was first placed on a countertop and the jet ejector manifold was placed on top of the jet ejector base a 7. Under the air flow nozzle A5 of the assembly, a sample base pad B2 is placed, and then a sample platen B1 is placed on the sample base pad B2. The components are assembled together as a functional aid as shown in figure 2.
Then the aseptic purification of the auxiliary device is carried out: after the auxiliary device is assembled, the super clean bench is firstly operated for more than 5 minutes, so that the inner space of the super clean bench is fully purified in a sterile manner, then the airflow engine A1 is powered on and started to operate, and the airflow engine sucks external sterile air and ejects sterile airflow through the airflow nozzle by virtue of the connecting pipeline. And at the moment, lightly knocking a member through which sterile airflow passes for many times by using a clean hard object, shaking off and taking away microorganisms or particles with weak adhesive force on the inner surface of the air passage, and then keeping the super clean bench and the airflow engine to operate for more than 10 minutes so as to fully and aseptically purify the inner air passage of the auxiliary device.
After the sterile purification of the auxiliary device, the dehydration of the sample material can be entered, as follows:
the sample material to be concentrated and dried is transferred into a suitable sample bottle (such as a common glass vaccine bottle, a common freezing tube and the like) in advance under an aseptic condition to prepare for dehydration. The operation method for entering the dehydration work is to open the sample bottle cap for loading the sample, place the sample bottle after being opened on the surface of the sample platform plate B1, slightly translate the sample bottle to the position right below the airflow nozzle A5, and increase the height of the bottom pad B2 of the sample platform, so that the airflow nozzle A5 is inserted into the sample bottle and is close to the liquid level in the bottle without contacting the sample bottle, as shown in figure 3. If the strength of the jet air flow of the spray head A5 is too high, which causes the liquid in the bottle to splash, a part of air release holes A8 on the air flow distribution and pressure regulation box A3 are opened, the air pressure in the box is reduced, and the jet air flow strength is appropriate; the non-liquid sample material can be omitted. The sample material is quickly evaporated, dehydrated, concentrated and dried under the action of the quick jet air flow.
The speed of evaporation dehydration is closely related to the technical conditions of the intensity, the temperature, the air humidity and the like of the jet air flow. The intensity of the jet air flow was appropriately increased by adjusting the air pressure of the air flow distribution and pressure adjustment box a3 without causing splashing of the sample liquid surface. The temperature of the jet air flow can be properly increased on the premise of not influencing the biological activity and the application of the sample material, such as adding a heating element in a workbench or an air flow injector. Conditionally minimizing the humidity of the ambient air, such as operating an air conditioner or dehumidifier.
After the dehydration of the sample material is completed, the sample base pad B2 is lowered, the airflow nozzle A5 is made to withdraw from the sample bottle, the sample bottle is moved slightly and taken out, and the sample bottle is covered under the aseptic condition in the super clean bench to obtain the concentrated and dried dehydrated sample material in the aseptic state. The air flow trigger a1 is then turned off and the power cord is unplugged. The components of the auxiliary device which can be freely disassembled are unloaded one by one and then are transferred back to a clean container for storage. And finally, shutting down the clean bench.
Example 1
The method is characterized in that a sterilized 10ml common glass vaccine bottle is used as a sample bottle, 5ml of sterile water is filled as a sample to be dehydrated, the auxiliary device for anti-pollution dehydration and the application method thereof are adopted to carry out dehydration treatment, the temperature of jet air flow is 38 ℃, and after dehydration treatment is carried out for 120 minutes, 5ml of water is completely evaporated and removed.
Then, 0.5ml of sterile water was poured into the dehydrated vial, the inside of the vial was washed by shaking, and then the vial was sampled 20 times from the washing water by a pipette and poured 10. mu.l at a time onto a PSA (Potato sucrose agar) plate, and the vial was incubated at 28 ℃ for 5 days and then taken out and observed to have a contamination ratio of 0.
Example 2
The method is characterized in that a sterilized 10ml common glass vaccine bottle is used as a sample bottle, 5ml sterilized skimmed milk is filled as a sample to be dehydrated, the auxiliary device for anti-pollution dehydration and the application method thereof are adopted, dehydration treatment is carried out, the temperature of jet air flow is 38 ℃, after dehydration treatment is carried out for 120 minutes, the sample in the bottle is changed into solid concentrated milk, the dehydration treatment is continued for 60 minutes, and the concentrated milk in the bottle is dried.
Then, 0.5ml of sterile water was poured into the dehydrated vial, the inside of the vial was washed with shaking to dissolve a part of the skim milk, and then the washed skim milk was sampled 20 times from the skim milk by a pipette gun, 10. mu.l of each sample was dropped onto a PSA (Potato sucrose agar) plate, and the plate was cultured at 28 ℃ for 5 days and then taken out and observed to have a contamination ratio of 0.
Example 3
A sterilized 10ml common glass vaccine bottle is used as a sample bottle, 1ml of 10% sterilized defatted milk is filled, a ring of ralstonia solanacearum is taken by a common transplanting ring and is transplanted into the 1ml sterilized defatted milk, and the sample to be dehydrated is fully and uniformly mixed. The auxiliary device for anti-pollution dehydration and the application method thereof are adopted to carry out dehydration treatment, the temperature of the jet air flow is 38 ℃, and the sample in the bottle becomes solid concentrate after 40 minutes of dehydration treatment. The dehydration treatment was continued for 50 minutes and the concentrate in the bottle was already dry.
Then 0.5ml of sterile water is injected into the small sample bottle after dehydration to dissolve part of the dehydrated dried substance, a ring of the dissolved substance is taken out by a common transplanting ring and is streaked on a potato sucrose agar culture medium plate, the culture medium plate is taken out after 2 days of culture at 28 ℃ and observed, clear viscous and milky bacterial colonies of the ralstonia solanacearum are grown at the streaked part of the culture plate, and the ralstonia solanacearum after the dehydration treatment can still keep normal activity.
Claims (2)
1. An auxiliary device for anti-pollution dehydration is characterized in that the auxiliary device mainly comprises an airflow ejector (A) and a sample table (B);
the air flow ejector (A) part comprises a component air flow engine (A1), an air flow connecting pipe (A2), an air flow distribution and pressure regulation box (A3), an air duct (A4), an air flow spray head (A5), an air flow ejector fixing plate (A6) and an air flow ejector base (A7); the component airflow distribution and pressure regulation box (A3) is provided with an airflow distribution structure and an air pressure regulation structure;
the air flow engine (A1), the air flow connecting pipe (A2), the air flow distribution and pressure regulation box (A3), the air guide pipe (A4), the air flow spray head (A5) and the ejector fixing plate (A6) are firmly connected together to form an air flow ejector assembly;
the sample stage (B) component comprises a component sample stage plate (B1) and a sample stage bottom pad (B2).
2. The method of use of an anti-contamination dewatering aid of claim 1, wherein the aid is used in conjunction with a clean bench, referred to as "clean bench"; the application method comprises the following steps:
1) starting and operating the super clean bench: normally cleaning an ultra-clean platform according to a conventional procedure, then starting up the ultra-clean platform to run and adjusting to the sterile air flow rate which is normally used;
2) assembling the auxiliary device: the auxiliary device is assembled in the running state of the super clean bench, and the assembling operation is as follows:
firstly, transferring the components into a super clean bench: taking out the components of the auxiliary device from the storage container and transferring the components into a super clean bench; drawing the power supply lead of the airflow engine (A1) out of the clean bench;
purifying the surface of the component: in the running super clean bench, purifying the surfaces of all components of the auxiliary device in a flapping vibration mode;
③ Special purification of the air flow nozzle (A5): specially cleaning the outer surface of the air flow nozzle (A5) with 75% alcohol and sterilized moist gauze;
assembling the components: firstly, placing an airflow ejector base (A7) on a working table, placing an airflow ejector assembly on the airflow ejector base (A7), placing a sample bottom pad (B2) below an airflow spray head (A5) of the assembly, and placing a sample bedplate (B1) on the sample bottom pad (B2);
3) sterile purification of the auxiliary device: and 2) after the operation is finished, performing sterile purification on the auxiliary device according to the following operations:
firstly, continuously operating the super clean bench for more than 5 minutes;
secondly, switching on a power supply and starting a running airflow engine (A1); sterile air is drawn into the air flow ejector internal conduit and ejected from the air flow nozzle (A5);
knocking each component through which sterile air flow passes in the auxiliary device to promote the purification of the internal air passage;
keeping the ultra-clean bench and the airflow engine (A1) running for more than 10 minutes to ensure the sterile purification of the internal air passage of the auxiliary device;
4) placing of sample material: after the operation of the step 3), placing a sample material according to the following operation:
firstly, transferring a sample material to be concentrated and dried into a proper sample bottle in advance;
secondly, opening the bottle cap of the sample bottle in the first operation, placing the uncapped sample bottle on the surface of a sample bedplate (B1), and moving the sample bottle to a position right below an airflow spray head (A5);
thirdly, the sample bottle is lifted, and the airflow nozzle (A5) is inserted into the sample bottle and is close to the liquid level of the sample in the bottle;
adjusting the sterile airflow sprayed by the airflow nozzle (A5) to a proper intensity;
5) and (3) operation of dehydration: keeping the working state of the step 4), continuously spraying the sample material by the sterile air flow sprayed by the air flow spray head (A5) of the auxiliary device, and leading the sample material to be quickly dehydrated, concentrated and dried;
6) and (5) finishing the work: step 5), after the dehydration is finished, taking out the sample bottle, and obtaining a dehydrated sample material after the cover is returned; shutting down the air flow engine (a 1); the components of the auxiliary device which can be freely disassembled are unloaded one by one and are transferred back to a clean container for storage; and finally, shutting down the clean bench.
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| DE69514743D1 (en) * | 1994-09-27 | 2000-03-02 | Saint Gobain Vitrage | Device for distributing powdery solids on the surface of a substrate for coating this substrate |
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| CN105318666A (en) * | 2015-11-17 | 2016-02-10 | 上海东富龙科技股份有限公司 | Vacuum spray freeze-drying device and method |
| CN207266860U (en) * | 2017-08-15 | 2018-04-24 | 柳州市妇幼保健院 | A kind of Multifunctional super-clean bench |
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2020
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE69514743D1 (en) * | 1994-09-27 | 2000-03-02 | Saint Gobain Vitrage | Device for distributing powdery solids on the surface of a substrate for coating this substrate |
| CN101287449A (en) * | 2002-04-11 | 2008-10-15 | 米迪缪尼疫苗股份有限公司 | Preservation of bioactive materials by spray drying |
| CN1472331A (en) * | 2003-06-19 | 2004-02-04 | 上海交通大学 | Method for testing virus removing effect with air purifier |
| CN102589948A (en) * | 2012-02-29 | 2012-07-18 | 缪应江 | Biological sample processing box |
| CN105318666A (en) * | 2015-11-17 | 2016-02-10 | 上海东富龙科技股份有限公司 | Vacuum spray freeze-drying device and method |
| CN207266860U (en) * | 2017-08-15 | 2018-04-24 | 柳州市妇幼保健院 | A kind of Multifunctional super-clean bench |
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