CN111793142A - Ultrasonic-assisted synergistic compound enzyme extraction process for polygonatum sibiricum polysaccharide - Google Patents
Ultrasonic-assisted synergistic compound enzyme extraction process for polygonatum sibiricum polysaccharide Download PDFInfo
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Abstract
The invention discloses an ultrasonic-assisted synergistic complex enzyme extraction process of polygonatum polysaccharide, which comprises the following steps: s1, selecting high-quality rhizoma polygonati; s2, weighing 10g of rhizoma polygonati powder through an electronic balance, adding the weighed 10g of rhizoma polygonati powder into a 500mL conical flask with a plug, adding a disodium hydrogen phosphate citric acid buffer solution with a certain pH value into the 500mL conical flask with the plug according to a certain liquid-material ratio, and uniformly stirring; s3, putting 500mL of conical flask with a plug into an HH-1 digital display constant-temperature water bath kettle, then adding a proper amount of cellulase and pectinase into the 500mL of conical flask with the plug, adjusting the temperature of the HH-1 digital display constant-temperature water bath kettle to 50 ℃, and promoting enzymolysis of rhizoma polygonati powder in the 500mL of conical flask with the plug by the cellulase and the pectinase under the condition of 50 ℃; the soluble polygonatum polysaccharide is extracted from polygonatum by taking polygonatum as a raw material, performing compound enzymolysis by using cellulase and pectinase and performing ultrasonic-assisted synergy, so that the extraction rate of the polygonatum polysaccharide is improved.
Description
Technical Field
The invention relates to the technical field of extraction of polygonatum polysaccharides, in particular to an ultrasonic-assisted synergistic complex enzyme extraction process of polygonatum polysaccharides.
Background
Rhizoma Polygonati is a plant of Polygonatum, belongs to medicinal and edible Chinese herbal medicine, and is mainly distributed in Heilongjiang, Liaoning, Shanxi, Henan, etc. Rhizoma Polygonati can be used for treating spleen and stomach weakness, dry cough with little phlegm, and deficiency of essence and blood, and also has effects of lowering blood pressure, preventing atherosclerosis, and delaying aging.
The rhizoma Polygonati contains polysaccharide, alkaloid, amino acids, etc. The polygonatum polysaccharide is one of the main active ingredients of polygonatum, has pharmacological effects of enhancing immune function, inhibiting bacteria, resisting inflammation, regulating blood sugar and blood fat and the like, and has good application prospects in the aspects of food, medical care and the like. Research shows that the polygonatum polysaccharide has a certain repairing or recovering effect on low immunity caused by cyclophosphamide, can remarkably promote the formation of serum hemolysin and promote the transformation of lymphocytes. Researches find that polygonatum cyrtonema polysaccharide can obviously reduce the death rate of type I diabetic mice, and is possibly related to the functions of improving the weight of the mice, reducing the blood sugar, protecting the liver and the like. Therefore, the method for extracting the polygonatum polysaccharide is important to find an economically suitable extraction method.
At present, the water extraction and alcohol precipitation method, the ultrasonic auxiliary method and the like are mostly adopted to extract polysaccharide in China, but the problem of low polysaccharide yield generally exists. In recent years, the enzyme method has the advantages of low reaction temperature, high efficiency, no pollution and the like, and is widely applied to the extraction of polysaccharide.
Disclosure of Invention
The invention aims to provide an ultrasonic-assisted synergistic complex enzyme extraction process of polygonatum polysaccharide, which aims to solve the problems in the background art.
In order to achieve the purpose, the invention provides the following technical scheme: an ultrasonic-assisted synergistic compound enzyme extraction process of polygonatum polysaccharide comprises the following steps:
s1, selecting high-quality rhizoma polygonati, then placing the selected rhizoma polygonati into a vacuum drying oven, drying the rhizoma polygonati through the vacuum drying oven, then adding the dried rhizoma polygonati into a pulverizer, pulverizing the rhizoma polygonati into powder through the pulverizer, then filtering large-particle impurities in the powder rhizoma polygonati through an 80-mesh filter screen, and hermetically storing the obtained rhizoma polygonati powder for later use;
s2, weighing 10g of rhizoma polygonati powder through an electronic balance, adding the weighed 10g of rhizoma polygonati powder into a 500mL conical flask with a plug, adding a disodium hydrogen phosphate citric acid buffer solution with a certain pH value into the 500mL conical flask with the plug according to a certain liquid-material ratio, and uniformly stirring;
s3, putting 500mL of conical flask with a plug into an HH-1 digital display constant-temperature water bath kettle, then adding a proper amount of cellulase and pectinase into the 500mL of conical flask with the plug, adjusting the temperature of the HH-1 digital display constant-temperature water bath kettle to 50 ℃, and promoting enzymolysis of rhizoma polygonati powder in the 500mL of conical flask with the plug by the cellulase and the pectinase under the condition of 50 ℃;
s4, placing 500mL of conical bottles with stoppers after enzymolysis into an ultrasonic cleaner, treating the 500mL of conical bottles with stoppers in the ultrasonic cleaner for 30 minutes, sequentially performing hot water extraction and suction filtration, and collecting filtrate to obtain rhizoma polygonati extract;
s5, adding a proper amount of diethyl ether into the rhizoma polygonati extract, shaking for 15 minutes, placing the shaken rhizoma polygonati extract into a separating funnel, standing for separating liquid, and collecting the lower-layer polysaccharide aqueous solution to remove fat-soluble impurities;
s6, adding the collected polysaccharide aqueous solution into a RE-52 rotary evaporator, carrying out rotary concentration treatment on the polysaccharide aqueous solution through the RE-52 rotary evaporator, then adding sufficient ethanol solution into the concentrated polysaccharide aqueous solution, standing overnight for 12 hours at 4 ℃, then adding the polysaccharide aqueous solution standing overnight into a centrifuge, centrifuging for 15 minutes through the centrifuge to obtain a precipitate, and drying the precipitate to obtain a crude polysaccharide sample of rhizoma polygonati;
s7, dissolving crude polygonatum sibiricum polysaccharide by using a proper amount of distilled water, fixing the volume of a polysaccharide solution obtained by dissolving to 250mL, accurately transferring 1mL of polysaccharide solution into a 250mL volumetric flask, adding 1mL of 5% phenol aqueous solution and 5mL of concentrated sulfuric acid into the volumetric flask to fix the volume, shaking uniformly, standing for 30 minutes, measuring absorbance at 490nm by using a UV-1800PC-DS2 ultraviolet-visible spectrophotometer, and performing analysis and calculation by using a glucose standard curve to obtain the polygonatum sibiricum polysaccharide yield.
In the step S1, the vacuum drying oven adopts a DZF-6050 vacuum drying oven, and the vacuum drying temperature of the vacuum drying oven is 60 ℃.
Wherein, in the step S1, the pulverizer adopts an HK-02A multifunctional pulverizer.
Wherein, in the step S2, the electronic balance adopts an LE204E electronic balance.
Wherein in the step S2, the liquid-to-material ratio of the disodium hydrogen phosphate citric acid buffer solution to the sealwort powder is 21:1 (mL/g).
Wherein, in the step S2, the pH value of the disodium hydrogen phosphate citric acid buffer solution is 6.0.
Wherein, in the step S3, the addition amount of the cellulase and the pectinase is 1.6%.
In step S4, the ultrasonic cleaner is a high-power numerical control ultrasonic cleaner, and the working power of the ultrasonic cleaner is 362W.
In the step S6, the centrifuge adopts a TDL-5-A low-speed desk type large-capacity centrifuge, and the rotation speed of the centrifuge is 4000 r/min.
Compared with the prior art, the invention has the beneficial effects that:
the soluble polygonatum polysaccharide is extracted from polygonatum by taking polygonatum as a raw material, performing compound enzymolysis by using cellulase and pectinase and performing ultrasonic-assisted synergy, so that the extraction rate of the polygonatum polysaccharide is improved.
Drawings
Fig. 1 shows the interaction effect of ultrasonic power of 360W and enzymolysis pH of 5 on the yield of polygonatum polysaccharide;
FIG. 2 shows the interaction effect of enzyme addition amount of 1.5% and enzymolysis pH of 5 on the yield of rhizoma Polygonati polysaccharide;
FIG. 3 shows the interaction effect of the enzyme dosage of 1.5% and the ultrasonic power of 360W on the yield of polygonatum polysaccharide;
FIG. 4 shows the interaction effect of pH 5 on the yield of Polygonatum sibiricum polysaccharide when the liquid-to-material ratio is 20: 1;
FIG. 5 shows the interaction effect of the liquid-material ratio of 20:1 and the ultrasonic power of 360W on the yield of polygonatum polysaccharide;
FIG. 6 shows the interaction effect of the liquid-to-feed ratio of 20:1 and the enzyme addition amount of 1.5% on the yield of polygonatum polysaccharide;
FIG. 7 is a line graph showing the influence of liquid-to-feed ratio on the yield of polygonatum polysaccharides;
FIG. 8 is a line graph showing the effect of different enzyme dosages on the yield of polygonatum polysaccharides;
FIG. 9 is a line graph showing the effect of ultrasonic power on polysaccharide yield;
FIG. 10 is a line graph showing the effect of enzymatic pH on polysaccharide yield.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The embodiment of the invention provides the following technical scheme: an ultrasonic-assisted synergistic compound enzyme extraction process of polygonatum polysaccharide comprises the following steps:
s1, selecting high-quality rhizoma polygonati, then placing the selected rhizoma polygonati into a vacuum drying oven, drying the rhizoma polygonati through the vacuum drying oven, then adding the dried rhizoma polygonati into a pulverizer, pulverizing the rhizoma polygonati into powder through the pulverizer, then filtering large-particle impurities in the powder rhizoma polygonati through an 80-mesh filter screen, and hermetically storing the obtained rhizoma polygonati powder for later use;
s2, weighing 10g of rhizoma polygonati powder through an electronic balance, adding the weighed 10g of rhizoma polygonati powder into a 500mL conical flask with a plug, adding a disodium hydrogen phosphate citric acid buffer solution with a certain pH value into the 500mL conical flask with the plug according to a certain liquid-material ratio, and uniformly stirring;
s3, putting 500mL of conical flask with a plug into an HH-1 digital display constant-temperature water bath kettle, then adding a proper amount of cellulase and pectinase into the 500mL of conical flask with the plug, adjusting the temperature of the HH-1 digital display constant-temperature water bath kettle to 50 ℃, and promoting enzymolysis of rhizoma polygonati powder in the 500mL of conical flask with the plug by the cellulase and the pectinase under the condition of 50 ℃;
s4, placing 500mL of conical bottles with stoppers after enzymolysis into an ultrasonic cleaner, treating the 500mL of conical bottles with stoppers in the ultrasonic cleaner for 30 minutes, sequentially performing hot water extraction and suction filtration, and collecting filtrate to obtain rhizoma polygonati extract;
s5, adding a proper amount of diethyl ether into the rhizoma polygonati extract, shaking for 15 minutes, placing the shaken rhizoma polygonati extract into a separating funnel, standing for separating liquid, and collecting the lower-layer polysaccharide aqueous solution to remove fat-soluble impurities;
s6, adding the collected polysaccharide aqueous solution into a RE-52 rotary evaporator, carrying out rotary concentration treatment on the polysaccharide aqueous solution through the RE-52 rotary evaporator, then adding sufficient ethanol solution into the concentrated polysaccharide aqueous solution, standing overnight for 12 hours at 4 ℃, then adding the polysaccharide aqueous solution standing overnight into a centrifuge, centrifuging for 15 minutes through the centrifuge to obtain a precipitate, and drying the precipitate to obtain a crude polysaccharide sample of rhizoma polygonati;
s7, dissolving crude polygonatum sibiricum polysaccharide by using a proper amount of distilled water, fixing the volume of a polysaccharide solution obtained by dissolving to 250mL, accurately transferring 1mL of polysaccharide solution into a 250mL volumetric flask, adding 1mL of 5% phenol aqueous solution and 5mL of concentrated sulfuric acid into the volumetric flask to fix the volume, shaking uniformly, standing for 30 minutes, measuring absorbance at 490nm by using a UV-1800PC-DS2 ultraviolet-visible spectrophotometer, and performing analysis and calculation by using a glucose standard curve to obtain the polygonatum sibiricum polysaccharide yield.
Wherein, in the step S1, the vacuum drying oven adopts a DZF-6050 vacuum drying oven, and the vacuum drying temperature of the vacuum drying oven is 60 ℃.
Wherein, in the step S1, the pulverizer adopts a HK-02A multifunctional pulverizer.
Wherein, in the step S2, the electronic balance adopts an LE204E electronic balance.
Wherein, in the step S2, the liquid-to-material ratio of the disodium hydrogen phosphate citric acid buffer solution to the sealwort powder is 21:1 (mL/g).
In step S2, the pH of the disodium hydrogenphosphate citric acid buffer solution was 6.0.
In step S3, the addition amount of cellulase and pectinase was 1.6%.
In step S4, the ultrasonic cleaner is a high-power numerical control ultrasonic cleaner, and the operating power of the ultrasonic cleaner is 362W.
In the step S6, the centrifuge adopts a TDL-5-A low-speed desk type large-capacity centrifuge, and the rotation speed of the centrifuge is 4000 r/min.
Wherein the content of the first and second substances,
in the formula: m is the mass (mg) of rhizoma Polygonati powder; a490 is the absorbance of the diluted polysaccharide solution; the former 250 is the dilution factor; the latter 250 is the volume (mL) of the polygonatum polysaccharide solution.
Wherein, the influence factors of the extraction of the polygonatum polysaccharide mainly comprise liquid-material ratio, enzyme adding amount, ultrasonic power and enzymolysis PH, and a single-factor comparison test is carried out according to different influence factors, which is detailed as follows:
1. influence of liquid-material ratio on yield of rhizoma Polygonati polysaccharide;
under the conditions of enzyme addition amount of 1.5% (m cellulase: m pectinase is 1: 1), ultrasonic power of 320W and enzymolysis pH of 5.0, the influence of different liquid-material ratios on the yield of the polygonatum polysaccharide is investigated, and the results are measured in parallel for 3 times.
As can be seen from FIG. 7, the yield of polysaccharide increases and then decreases with the increase of the liquid-to-feed ratio, and the yield of polysaccharide is the highest when the liquid-to-feed ratio is 20: 1. The reason for this is probably that the greater the concentration difference of polysaccharide inside and outside the cells of the polygonatum sibiricum is along with the increase of the liquid-material ratio, the greater the mass transfer driving force is, the easier the polygonatum sibiricum polysaccharide is leached; when the feed-liquid ratio is too high, the contact between the sample and the enzyme is reduced, and the yield of the polysaccharide is influenced.
2. Influence of enzyme dosage on yield of rhizoma Polygonati polysaccharide;
under the conditions of liquid-material ratio of 20:1, ultrasonic power of 320W and enzymolysis pH of 5.0, the influence of different enzyme addition amounts on the yield of the polygonatum polysaccharide is examined, and the results are parallelly determined for 3 times.
As can be seen from FIG. 8, when the enzyme dosage is 0.5% -1.5%, the yield of the polygonatum polysaccharide is gradually increased, and when the enzyme dosage is higher than 1.5%, the polysaccharide yield tends to be stable.
3. Influence of ultrasonic power on the yield of polygonatum polysaccharide;
under the conditions of enzyme addition amount of 1.5% (m cellulase: m pectinase is 1: 1), liquid-material ratio of 20:1 and enzymolysis pH of 5.0, the influence of ultrasonic power on the yield of polygonatum polysaccharide is examined, and each test is carried out for 3 times in parallel.
As shown in fig. 9, the yield of polygonatum polysaccharide is gradually increased with the increase of the ultrasonic power, and the yield of polygonatum polysaccharide is almost unchanged when the ultrasonic power is greater than 360W. This is because the higher the ultrasonic power, the stronger the ultrasonic wave has to break the cell wall, the higher the dissolution rate of intracellular polysaccharide, and the faster the molecular diffusion on the interface diffusion layer.
4. Influence of enzymolysis pH on the yield of polygonatum polysaccharide;
under the conditions of enzyme addition amount of 1.5% (m cellulase: m pectinase is 1: 1), liquid-material ratio of 20:1 and ultrasonic power of 320W, the influence of enzymolysis pH on the yield of the polygonatum polysaccharide is investigated, and each test is carried out for 3 times in parallel.
As shown in FIG. 10, when the pH value of the enzymolysis is in the range of 4.0-6.0, the yield of the polygonatum polysaccharide is high. The optimum pH value of the cellulase is 4.5-5.5, the pH value of the pectinase is 2.5-6.0, the cellulase is suitable for product generation, and the activity of the cellulase is reduced when the pH value is too high or too low, the cell wall breaking degree is reduced, and the exudation of intracellular polysaccharide is influenced.
Establishing a mathematical model of the yield of the polygonatum polysaccharide:
TABLE 1 factor level coding table for Box-Behnken center combinatorial design
Based on the above single factor test results. Selecting four factors which have obvious influence on the yield of the polygonatum polysaccharide, namely a liquid-material ratio (A), an enzyme adding amount (B), ultrasonic power (C) and enzymolysis pH (D), taking the polygonatum polysaccharide yield as a response value (Y), and further optimizing the polygonatum polysaccharide extraction process by adopting a Box-Benhnken test design method. The analytical factors and the level codes are shown in Table 1, and the experimental protocol and results are shown in Table 2.
TABLE 2 Box-Benhnken test design and results
According to the experimental results in the table 2, a regression equation influencing the polygonatum yield is obtained by fitting Design-Expert 10.0.3 software: the yield Y is 27.68+0.46A +0.75B +0.34C +0.55D +0.13AB-0.30AC-0.096AD +0.14BC-0.26BD-0.058CD-0.86A2-3.38B2-2.37C2-4.08D2
TABLE 3 Box-Behnken test results analysis of variance
Note: the difference is extremely significant, P < 0.001; significant difference height, P < 0.01; differences were significant, P < 0.05.
Note：significant，P＜0.05。
As can be seen from Table 3, the F value of the established model is 27.85(P is less than 0.0001), which indicates that the model is extremely obvious, the P value of the mismatching term is 0.2836 is more than 0.05, which indicates that the model can well fit the real situation of the test, and the model can be used for the optimization of the extraction process of the polygonatum polysaccharide. Wherein R is2Value 0.9653, corrected to determine the coefficient R2 AdjA value of 0.9307, illustrating the modelIt is possible to interpret the change in response value of 93.07% of the experimental data. In addition, B2、C2、D2The effect on the extraction amount of polysaccharide is very different, B, A2The influence on the polysaccharide extraction amount is highly significant, A, D has significant influence on the polysaccharide extraction amount, C, AB, AC, AD, BC, BD and CD terms are not significant (P is more than 0.05), and 7 terms are deleted from the regression equation. Thus, the regression equation ends up with a yield Y of 27.68+0.46A +0.75B +0.55D-0.86A2-3.38B2-2.37C2-4.08D2。
The response surface analysis of the polygonatum polysaccharide yield is detailed as follows: figures 1-6 of the specification;
fig. 1 to 6 show the interactive influence of the effect of each influencing factor on the polygonatum polysaccharide yield, the 6 response surface graphs are convex surfaces with downward openings, the polygonatum polysaccharide yield has different changing trends along with the change of the four factors, but the enzyme addition has the largest influence on the polygonatum polysaccharide yield through the analysis of the response surface, the response surface is the steepest, and then the influencing factors are enzymolysis pH, liquid-to-material ratio and ultrasonic power in turn, and the result is consistent with the analysis result of variance.
And (3) model verification:
in consideration of controllability of experimental operation, the above-described optimum experimental conditions are appropriately modified: the liquid-material ratio is 21:1(mL/g), the enzyme addition amount is 1.6%, the ultrasonic power is 360W, and the enzymolysis pH is 6.0, 3 groups of parallel experiments are carried out, the actually measured average yield of the polygonatum polysaccharide is 28.02%, and is closer to the predicted value of 27.81%, and the rationality of the regression model of the response surface method is verified.
In summary, the following steps: the invention takes rhizoma polygonati as a raw material, the soluble rhizoma polygonati polysaccharide is extracted from the rhizoma polygonati through compound enzymolysis of cellulase and pectinase and ultrasonic wave auxiliary cooperation, the extraction rate of the rhizoma polygonati polysaccharide is improved, the optimal process parameters are obtained by adopting a response surface method, and the optimal extraction process conditions are determined as follows: 10g of polygonatum sibiricum powder, the liquid-material ratio is 21:1(mL/g), the enzyme adding amount is 1.6%, the ultrasonic power is 362W, the enzymolysis pH is 6.0, and the yield of polygonatum sibiricum polysaccharide is 28.02%.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (9)
1. An ultrasonic-assisted synergistic complex enzyme extraction process of polygonatum polysaccharide is characterized by comprising the following steps:
s1, selecting high-quality rhizoma polygonati, then placing the selected rhizoma polygonati into a vacuum drying oven, drying the rhizoma polygonati through the vacuum drying oven, then adding the dried rhizoma polygonati into a pulverizer, pulverizing the rhizoma polygonati into powder through the pulverizer, then filtering large-particle impurities in the powder rhizoma polygonati through an 80-mesh filter screen, and hermetically storing the obtained rhizoma polygonati powder for later use;
s2, weighing 10g of rhizoma polygonati powder through an electronic balance, adding the weighed 10g of rhizoma polygonati powder into a 500mL conical flask with a plug, adding a disodium hydrogen phosphate citric acid buffer solution with a certain pH value into the 500mL conical flask with the plug according to a certain liquid-material ratio, and uniformly stirring;
s3, putting 500mL of conical flask with a plug into an HH-1 digital display constant-temperature water bath kettle, then adding a proper amount of cellulase and pectinase into the 500mL of conical flask with the plug, adjusting the temperature of the HH-1 digital display constant-temperature water bath kettle to 50 ℃, and promoting enzymolysis of rhizoma polygonati powder in the 500mL of conical flask with the plug by the cellulase and the pectinase under the condition of 50 ℃;
s4, placing 500mL of conical bottles with stoppers after enzymolysis into an ultrasonic cleaner, treating the 500mL of conical bottles with stoppers in the ultrasonic cleaner for 30 minutes, sequentially performing hot water extraction and suction filtration, and collecting filtrate to obtain rhizoma polygonati extract;
s5, adding a proper amount of diethyl ether into the rhizoma polygonati extract, shaking for 15 minutes, placing the shaken rhizoma polygonati extract into a separating funnel, standing for separating liquid, and collecting the lower-layer polysaccharide aqueous solution to remove fat-soluble impurities;
s6, adding the collected polysaccharide aqueous solution into a RE-52 rotary evaporator, carrying out rotary concentration treatment on the polysaccharide aqueous solution through the RE-52 rotary evaporator, then adding sufficient ethanol solution into the concentrated polysaccharide aqueous solution, standing overnight for 12 hours at 4 ℃, then adding the polysaccharide aqueous solution standing overnight into a centrifuge, centrifuging for 15 minutes through the centrifuge to obtain a precipitate, and drying the precipitate to obtain a crude polysaccharide sample of rhizoma polygonati;
s7, dissolving crude polygonatum sibiricum polysaccharide by using a proper amount of distilled water, fixing the volume of a polysaccharide solution obtained by dissolving to 250mL, accurately transferring 1mL of polysaccharide solution into a 250mL volumetric flask, adding 1mL of 5% phenol aqueous solution and 5mL of concentrated sulfuric acid into the volumetric flask to fix the volume, shaking uniformly, standing for 30 minutes, measuring absorbance at 490nm by using a UV-1800PC-DS2 ultraviolet-visible spectrophotometer, and performing analysis and calculation by using a glucose standard curve to obtain the polygonatum sibiricum polysaccharide yield.
2. The ultrasonic-assisted synergistic complex enzyme extraction process of polygonatum polysaccharide according to claim 1, which is characterized in that: in the step S1, the vacuum drying oven adopts a DZF-6050 vacuum drying oven, and the vacuum drying temperature of the vacuum drying oven is 60 ℃.
3. The ultrasonic-assisted synergistic complex enzyme extraction process of polygonatum polysaccharide according to claim 1, which is characterized in that: in the step S1, the pulverizer adopts a HK-02A multifunctional pulverizer.
4. The ultrasonic-assisted synergistic complex enzyme extraction process of polygonatum polysaccharide according to claim 1, which is characterized in that: in step S2, the electronic balance employs an LE204E electronic balance.
5. The ultrasonic-assisted synergistic complex enzyme extraction process of polygonatum polysaccharide according to claim 1, which is characterized in that: in the step S2, the liquid-to-material ratio of the disodium hydrogen phosphate citric acid buffer solution to the sealwort powder is 21:1 (mL/g).
6. The ultrasonic-assisted synergistic complex enzyme extraction process of polygonatum polysaccharide according to claim 1, which is characterized in that: in step S2, the PH of the disodium hydrogen phosphate citrate buffer is 6.0.
7. The ultrasonic-assisted synergistic complex enzyme extraction process of polygonatum polysaccharide according to claim 1, which is characterized in that: in step S3, the cellulase and pectinase were added in an amount of 1.6%.
8. The ultrasonic-assisted synergistic complex enzyme extraction process of polygonatum polysaccharide according to claim 1, which is characterized in that: in step S4, the ultrasonic cleaner is a high-power numerical control ultrasonic cleaner, and the working power of the ultrasonic cleaner is 362W.
9. The ultrasonic-assisted synergistic complex enzyme extraction process of polygonatum polysaccharide according to claim 1, which is characterized in that: in the step S6, the centrifuge adopts a TDL-5-A low-speed desk type large-capacity centrifuge, and the rotation speed of the centrifuge is 4000 r/min.
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| CN112220883A (en) * | 2020-10-22 | 2021-01-15 | 贵州蔚源中药材产业开发有限公司 | Sealwort processing technology |
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| CN112608391A (en) * | 2020-12-10 | 2021-04-06 | 贵州黔峰百草科技发展有限公司 | Extraction and separation process of rhizoma polygonati |
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| CN112220883A (en) * | 2020-10-22 | 2021-01-15 | 贵州蔚源中药材产业开发有限公司 | Sealwort processing technology |
| CN112279934A (en) * | 2020-11-23 | 2021-01-29 | 内蒙古自治区药品检验研究院(内蒙古自治区化妆品检验检测中心、内蒙古自治区医疗器械检测中心) | Complex enzyme combined ultrasonic extraction process of cistanche polysaccharide |
| CN112608391A (en) * | 2020-12-10 | 2021-04-06 | 贵州黔峰百草科技发展有限公司 | Extraction and separation process of rhizoma polygonati |
| CN112841641A (en) * | 2021-01-26 | 2021-05-28 | 宁波健安进生物科技有限公司 | Instant polygonatum sibiricum crisp chips and preparation method thereof |
| CN112841641B (en) * | 2021-01-26 | 2022-03-15 | 宁波健安进生物科技有限公司 | Instant polygonatum sibiricum crisp chips and preparation method thereof |
| CN114957494A (en) * | 2021-02-23 | 2022-08-30 | 江苏农牧科技职业学院 | Process for extracting protein in polygonatum polysaccharide by adopting double aqueous phases |
| CN113041317A (en) * | 2021-03-25 | 2021-06-29 | 四川省圣土中药材种植有限公司 | Method for extracting rhizoma polygonati extract by adopting nine-steaming and nine-sun-drying process |
| CN114903131A (en) * | 2022-03-25 | 2022-08-16 | 绍兴文理学院 | Radix puerariae and rhizoma polygonati compound solid beverage with antioxidant activity and preparation method |
| CN115120567A (en) * | 2022-08-02 | 2022-09-30 | 信阳农林学院 | Method for improving content of effective ingredients in radix puerariae and rhizoma polygonati hypoglycemic chewable tablets |
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