CN114903131A - Radix puerariae and rhizoma polygonati compound solid beverage with antioxidant activity and preparation method - Google Patents
Radix puerariae and rhizoma polygonati compound solid beverage with antioxidant activity and preparation method Download PDFInfo
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- CN114903131A CN114903131A CN202210317129.XA CN202210317129A CN114903131A CN 114903131 A CN114903131 A CN 114903131A CN 202210317129 A CN202210317129 A CN 202210317129A CN 114903131 A CN114903131 A CN 114903131A
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- kudzu root
- radix puerariae
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- 235000013361 beverage Nutrition 0.000 title claims abstract description 30
- 150000001875 compounds Chemical class 0.000 title claims abstract description 28
- 239000007787 solid Substances 0.000 title claims abstract description 25
- 230000003078 antioxidant effect Effects 0.000 title claims abstract description 17
- 238000002360 preparation method Methods 0.000 title claims abstract description 17
- 235000010575 Pueraria lobata Nutrition 0.000 claims abstract description 27
- 150000008442 polyphenolic compounds Chemical class 0.000 claims abstract description 12
- 235000013824 polyphenols Nutrition 0.000 claims abstract description 12
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 58
- 239000000843 powder Substances 0.000 claims description 26
- 238000001914 filtration Methods 0.000 claims description 25
- 244000046146 Pueraria lobata Species 0.000 claims description 24
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 19
- 239000000284 extract Substances 0.000 claims description 15
- 238000000605 extraction Methods 0.000 claims description 15
- 239000006228 supernatant Substances 0.000 claims description 15
- 241000037831 Polygonatum sibiricum Species 0.000 claims description 12
- 238000000967 suction filtration Methods 0.000 claims description 12
- 235000006200 Glycyrrhiza glabra Nutrition 0.000 claims description 8
- 238000006243 chemical reaction Methods 0.000 claims description 8
- 238000004108 freeze drying Methods 0.000 claims description 8
- 238000002156 mixing Methods 0.000 claims description 8
- 108090000790 Enzymes Proteins 0.000 claims description 7
- 102000004190 Enzymes Human genes 0.000 claims description 7
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- 238000007873 sieving Methods 0.000 claims description 7
- 238000009210 therapy by ultrasound Methods 0.000 claims description 7
- 238000001035 drying Methods 0.000 claims description 6
- 239000002244 precipitate Substances 0.000 claims description 6
- 150000004676 glycans Chemical class 0.000 claims description 5
- 229920001282 polysaccharide Polymers 0.000 claims description 5
- 239000005017 polysaccharide Substances 0.000 claims description 5
- 239000011347 resin Substances 0.000 claims description 5
- 229920005989 resin Polymers 0.000 claims description 5
- 235000001453 Glycyrrhiza echinata Nutrition 0.000 claims description 4
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- 238000004140 cleaning Methods 0.000 claims description 4
- 238000005520 cutting process Methods 0.000 claims description 4
- 238000002386 leaching Methods 0.000 claims description 4
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- 108010059820 Polygalacturonase Proteins 0.000 claims description 3
- 241000756042 Polygonatum Species 0.000 claims description 3
- 239000003463 adsorbent Substances 0.000 claims description 3
- 238000001816 cooling Methods 0.000 claims description 3
- 108010093305 exopolygalacturonase Proteins 0.000 claims description 3
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- 238000001179 sorption measurement Methods 0.000 claims description 2
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- 241000202807 Glycyrrhiza Species 0.000 claims 2
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- 239000000287 crude extract Substances 0.000 claims 1
- 230000003647 oxidation Effects 0.000 abstract description 11
- 238000007254 oxidation reaction Methods 0.000 abstract description 11
- HHEAADYXPMHMCT-UHFFFAOYSA-N dpph Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1[N]N(C=1C=CC=CC=1)C1=CC=CC=C1 HHEAADYXPMHMCT-UHFFFAOYSA-N 0.000 abstract description 9
- 230000000694 effects Effects 0.000 abstract description 9
- 239000003814 drug Substances 0.000 abstract description 7
- OHDRQQURAXLVGJ-HLVWOLMTSA-N azane;(2e)-3-ethyl-2-[(e)-(3-ethyl-6-sulfo-1,3-benzothiazol-2-ylidene)hydrazinylidene]-1,3-benzothiazole-6-sulfonic acid Chemical compound [NH4+].[NH4+].S/1C2=CC(S([O-])(=O)=O)=CC=C2N(CC)C\1=N/N=C1/SC2=CC(S([O-])(=O)=O)=CC=C2N1CC OHDRQQURAXLVGJ-HLVWOLMTSA-N 0.000 abstract description 6
- 229940079593 drug Drugs 0.000 abstract description 5
- 210000000952 spleen Anatomy 0.000 abstract description 4
- 210000001124 body fluid Anatomy 0.000 abstract description 3
- 239000010839 body fluid Substances 0.000 abstract description 3
- 238000004519 manufacturing process Methods 0.000 abstract description 3
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- 125000002887 hydroxy group Chemical group [H]O* 0.000 abstract description 2
- 241000219781 Pueraria montana var. lobata Species 0.000 abstract 3
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- 230000035764 nutrition Effects 0.000 abstract 1
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- 231100000419 toxicity Toxicity 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 35
- 235000019441 ethanol Nutrition 0.000 description 13
- 238000002835 absorbance Methods 0.000 description 9
- 239000000523 sample Substances 0.000 description 9
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 8
- 239000012153 distilled water Substances 0.000 description 8
- 150000003254 radicals Chemical class 0.000 description 7
- 244000303040 Glycyrrhiza glabra Species 0.000 description 6
- -1 ABTS radical Chemical class 0.000 description 5
- TUJKJAMUKRIRHC-UHFFFAOYSA-N hydroxyl Chemical compound [OH] TUJKJAMUKRIRHC-UHFFFAOYSA-N 0.000 description 5
- 235000010323 ascorbic acid Nutrition 0.000 description 4
- 229960005070 ascorbic acid Drugs 0.000 description 4
- 239000011668 ascorbic acid Substances 0.000 description 4
- 229940088598 enzyme Drugs 0.000 description 4
- LPLVUJXQOOQHMX-QWBHMCJMSA-N glycyrrhizinic acid Chemical compound O([C@@H]1[C@@H](O)[C@H](O)[C@H](O[C@@H]1O[C@@H]1C([C@H]2[C@]([C@@H]3[C@@]([C@@]4(CC[C@@]5(C)CC[C@@](C)(C[C@H]5C4=CC3=O)C(O)=O)C)(C)CC2)(C)CC1)(C)C)C(O)=O)[C@@H]1O[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@H]1O LPLVUJXQOOQHMX-QWBHMCJMSA-N 0.000 description 4
- 235000011477 liquorice Nutrition 0.000 description 4
- YGSDEFSMJLZEOE-UHFFFAOYSA-N salicylic acid Chemical compound OC(=O)C1=CC=CC=C1O YGSDEFSMJLZEOE-UHFFFAOYSA-N 0.000 description 4
- 241000721047 Danaus plexippus Species 0.000 description 3
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 3
- 239000002131 composite material Substances 0.000 description 3
- 230000007760 free radical scavenging Effects 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 230000002292 Radical scavenging effect Effects 0.000 description 2
- ATJFFYVFTNAWJD-UHFFFAOYSA-N Tin Chemical compound [Sn] ATJFFYVFTNAWJD-UHFFFAOYSA-N 0.000 description 2
- 239000003963 antioxidant agent Substances 0.000 description 2
- 235000006708 antioxidants Nutrition 0.000 description 2
- HEILIGJNYTWOHU-UHFFFAOYSA-N ethanol 2-hydroxybenzoic acid Chemical compound CCO.OC(=O)C1=CC=CC=C1O HEILIGJNYTWOHU-UHFFFAOYSA-N 0.000 description 2
- 239000011790 ferrous sulphate Substances 0.000 description 2
- 235000003891 ferrous sulphate Nutrition 0.000 description 2
- 239000000835 fiber Substances 0.000 description 2
- 238000000227 grinding Methods 0.000 description 2
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 2
- 229910000359 iron(II) sulfate Inorganic materials 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- FJKROLUGYXJWQN-UHFFFAOYSA-N papa-hydroxy-benzoic acid Natural products OC(=O)C1=CC=C(O)C=C1 FJKROLUGYXJWQN-UHFFFAOYSA-N 0.000 description 2
- USHAGKDGDHPEEY-UHFFFAOYSA-L potassium persulfate Chemical compound [K+].[K+].[O-]S(=O)(=O)OOS([O-])(=O)=O USHAGKDGDHPEEY-UHFFFAOYSA-L 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 229960004889 salicylic acid Drugs 0.000 description 2
- 239000012488 sample solution Substances 0.000 description 2
- 238000010025 steaming Methods 0.000 description 2
- 235000013616 tea Nutrition 0.000 description 2
- 238000002604 ultrasonography Methods 0.000 description 2
- 206010002383 Angina Pectoris Diseases 0.000 description 1
- 235000017060 Arachis glabrata Nutrition 0.000 description 1
- 244000105624 Arachis hypogaea Species 0.000 description 1
- 235000010777 Arachis hypogaea Nutrition 0.000 description 1
- 235000018262 Arachis monticola Nutrition 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- 108010059892 Cellulase Proteins 0.000 description 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- 241000893536 Epimedium Species 0.000 description 1
- 206010020772 Hypertension Diseases 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 235000007215 black sesame Nutrition 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 229940106157 cellulase Drugs 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 235000018905 epimedium Nutrition 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- WVLDCUJMGWFHGE-UHFFFAOYSA-L iron(2+);sulfate;hexahydrate Chemical compound O.O.O.O.O.O.[Fe+2].[O-]S([O-])(=O)=O WVLDCUJMGWFHGE-UHFFFAOYSA-L 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 235000020232 peanut Nutrition 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 239000012088 reference solution Substances 0.000 description 1
- 230000033764 rhythmic process Effects 0.000 description 1
- 229910052711 selenium Inorganic materials 0.000 description 1
- 239000011669 selenium Substances 0.000 description 1
- 229930002600 steroidal saponin Natural products 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/385—Concentrates of non-alcoholic beverages
- A23L2/39—Dry compositions
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/125—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives containing carbohydrate syrups; containing sugars; containing sugar alcohols; containing starch hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/90—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in food processing or handling, e.g. food conservation
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Mycology (AREA)
- Molecular Biology (AREA)
- Botany (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a kudzu root and rhizoma polygonati compound solid beverage with antioxidant activity and a preparation method thereof. And three in-vitro oxidation resistance model experiments are established to prove that the kudzu root polyphenol has higher clearance rate on DPPH, hydroxyl free radicals and ABTS, and simultaneously, the kudzu root and rhizoma polygonati compound beverage has higher oxidation resistance. The product mixes the three medicines, reduces toxicity, enhances efficacy, has the effects of nourishing yin, promoting the production of body fluid, invigorating spleen and replenishing qi, has a good antioxidation level, meets the requirements of people on health in the current society, and becomes a health beverage which can meet the pursuit of consumers on more health and nutrition.
Description
Technical Field
The invention relates to a formula of a solid beverage and a preparation method thereof, in particular to a kudzu root and rhizoma polygonati compound solid beverage with antioxidant activity and a preparation method thereof.
Background
Radix Puerariae contentPuerarin and its preparation methodSoybean, soybeanFlavonoid glycosideNutrient components such as peanut essence, protein, etc,Amino acidsSugar, and minerals essential to human body such as iron, calcium, copper, selenium, etc. in modern medicine, can be used for treating cardiovascular diseases such as angina pectoris, hypertension, and also has outstanding effect in inhibiting cancer. In addition, polyphenol in the kudzuvine root has strong oxidation resistance, and can remove free radicals generated in organisms, restore the activity of cells and inhibit the occurrence of various diseases. Therefore, the kudzu root plays a very important role in medicine and maintaining human health. Rhizoma polygonati contains steroidal saponins and polysaccharide substances, has the effects of tonifying qi and yin, promoting the production of body fluid and invigorating spleen, and is often used in a prescription formed by radix rehmanniae preparata, black sesame and other medicinal materials in recent years due to the effect of invigorating the spleen.
In the modern society, the living standard of people is improved, and the pressure and rhythm of life are increased, and meanwhile, the health care consciousness is also improved, including the aspect of the requirement of beverages in daily life. At present, solid tea beverage has become one of the inevitable trends due to its convenience. The common solid tea beverage in the market has single taste function, and cannot meet the requirement of consumers on daily health care, so the solid beverage with the health care function becomes one of the priority of people in the future.
Disclosure of Invention
The purpose of the invention is as follows: the invention aims to provide a kudzu root and sealwort compound solid beverage with oxidation resistance.
The invention also aims to provide a preparation method of the radix puerariae and rhizoma polygonati compound solid beverage with higher oxidation resistance.
The technical scheme is as follows: the preparation method of the kudzu root and sealwort compound solid beverage comprises the following steps:
(1) crude extraction of pueraria polyphenol:
a1, selecting materials and cleaning: cleaning radix Puerariae, and cutting into small pieces;
a2, crushing: pulverizing radix Puerariae, and sieving to obtain coarse powder;
a3, extraction: adding radix Puerariae powder into ethanol to desired volume, and performing ultrasonic treatment;
a4, filtering: centrifuging and filtering the extracting solution, and taking the supernatant for later use;
a5, extraction: adding ethanol into radix Puerariae precipitate, extracting, filtering, extracting repeatedly for 2-3 times, and mixing extractive solutions;
a6, suction filtration: filtering the mixed supernatant, and placing in a beaker for later use;
(2) extracting and separating kudzu root polyphenol:
b1, extraction: adding radix Puerariae powder into ethanol, and performing ultrasonic treatment;
b2, suction filtration: filtering the extract, and placing in a volumetric flask for later use;
b3, suction filtration: putting radix Puerariae extract into conical flask, adding macroporous adsorbent resin, stirring, standing, and vacuum filtering;
b4, suction filtration: adding the filter residue into ethanol, shaking and filtering;
b5, concentration: putting the filtrate into a round-bottom flask, and concentrating by using a rotary evaporator;
b6, freeze drying: freeze drying the concentrated liquid for later use;
(3) crude extraction of polygonatum polysaccharide
C1, drying: spreading rhizoma Polygonati in culture dish, and placing in oven;
c2, crushing: pulverizing rhizoma Polygonati into powder;
c3, leaching: adding the polygonatum sibiricum powder into a complex enzyme, carrying out enzymolysis, carrying out water bath, and continuing the reaction after heating;
c4, water bath: carrying out water bath reaction, and continuing the reaction after the temperature is increased;
c5, filtering: subpackaging the cooled solution, centrifuging, and mixing the supernatants;
c6, concentration: concentrating the supernatant with rotary evaporator;
(4) crude extraction of licorice:
d1, crushing: pulverizing Glycyrrhrizae radix into small pieces, and sieving.
The prepared kudzu root and sealwort compound solid beverage comprises the following raw materials: 26.74g of kudzu root polyphenol extract, 50mL of polygonatum polysaccharide extract and 49.10g of licorice powder. According to the principle of monarch, minister, assistant and guide, the kudzu root is taken as a monarch drug, the rhizoma polygonati is taken as a minister drug, and the liquorice is added to prepare the drugs, so that the antioxidant has better inoxidizability, and is shown in better clearance rate on DPPH free radicals, hydroxyl free radicals and ABTS free radicals.
Further, the extraction of a3 in the step (1): adding 55.0g radix Puerariae powder into 1000mL beaker, adding 70% ethanol to desired volume, and treating with ultrasound for 1h (350W, 32 deg.C).
Further, the extraction of a5 in the step (1): extracting the precipitate with 70% ethanol for 2-3 times.
Further, the extraction of B1 in the step (2): 50g of sieved kudzu root powder and 300mL of 70% ethanol are added into a 500mL beaker, and ultrasonic treatment is carried out for 1h at the temperature of 60 ℃.
Further, suction filtration of B3 in the step (2): 50mL of radix puerariae extract is added into a 100mL conical flask, 5g of pretreated macroporous adsorption resin is added according to the solid-to-liquid ratio of 1: 10 (g: mL), the mixture is stirred at room temperature, placed for 2 hours and filtered by suction.
Further, suction filtration of B4 in the step (2): adding 50mL 70% ethanol into the filter residue, shaking for 120min, and filtering.
Further, the freeze-drying of B6 in the step (2): freeze drying at-20 deg.C.
Further, the drying of C1 in the step (3): 50g of rhizoma polygonati are spread in a culture dish and placed in an oven at 40 ℃ for 48 hours.
Further, the leaching of C3 in the step (3): 10g of sealwort powder and 200mg of complex enzyme are added into a 500mL flask, and the complex enzyme is pectinase and cellulose.
Further, the water bath of C4 in step (3): the reaction was carried out in a water bath at 52 ℃ for 1h, and then the temperature was raised to 80 ℃ for 1 h.
Further, the filtration of C5 in the step (3): after cooling, the supernatant was placed in 4 centrifuge cups, centrifuged at 8000r/min, and the supernatant was mixed.
Further, the concentration of C6 in the step (3): the supernatant was concentrated to 50mL using a rotary evaporator.
Has the advantages that: compared with the prior art, the invention has the following advantages:
1. the mouthfeel is good: the kudzu root, the rhizoma polygonati and the liquorice are compounded to prepare the solid beverage, so that the effects of nourishing yin, promoting the production of body fluid, tonifying spleen and benefiting qi are improved.
2. The oxidation resistance is good: the invention tests the oxidation resistance of the compounded product, and proves that the compounded product has better oxidation resistance and certain health care effect.
Detailed Description
Example 1
Cutting cleaned radix Puerariae into small pieces, pulverizing in a pulverizer, sieving, and removing larger particles and fiber. 55.0g of kudzu root powder is added into a 1000mL beaker, and the volume is fixed to the mark by 70% ethanol. The mixture was sonicated for one hour (350W, 32 ℃ C.). Centrifuging at 5000r/min for 10min, and discarding precipitate. Extracting the precipitate with 70% ethanol for 2-3 times, mixing the extracted supernatants, rotary steaming with rotary steaming instrument, vacuum filtering, and placing in beaker. Sieving radix Puerariae powder, adding 50g into 500mL beaker, adding 300mL 70% ethanol, treating with ultrasound at 60 deg.C for 1h, filtering, and placing in 1000mL volumetric flask for use. Adding 50mL of radix Puerariae extract into 100mL conical flask, adding 5g of pretreated macroporous adsorbent resin according to solid-to-liquid ratio of 1: 10 (g: mL), stirring at room temperature, standing for 2h, and filtering. Adding 50mL 70% ethanol into the filter residue, shaking for 120min, and filtering again. The liquid is put into a round-bottom flask, and the liquid is concentrated to 60mL by using a rotary evaporator and is frozen and dried at the temperature of minus 20 ℃ for standby. 50g of rhizoma polygonati are flatly paved in a culture dish, placed in a drying oven at 40 ℃ for 48 hours and then pulverized into powder by a pulverizer. Adding 10g of rhizoma polygonati powder into a 500mL flask, adding 200mg of complex enzyme (pectinase 120mg and cellulase 80mg), reacting for 1h in a water bath at 52 ℃, raising the temperature to 80 ℃ and continuing to react for 1h, cooling the solution, then putting the solution into 4 centrifuge cups, carrying out centrifugal filtration, carrying out centrifugal machine rotation speed 8000r/min, mixing the supernatants in the 4 centrifuge cups, discarding residues, and concentrating to 50mL by using a rotary evaporator. Cutting 50g of liquorice into small pieces, grinding the small pieces in a grinding machine, sieving the powder and removing fiber parts.
Example 2
In order to prove the oxidation resistance of the compound solid beverage, the oxidation resistance of the radix puerariae and rhizoma polygonati compound is measured, and the oxidation resistance comprises DPPH clearance, hydroxyl radical clearance and ABTS radical clearance.
1. Research on DPPH clearance rate of radix puerariae and rhizoma polygonati compound
0.0078g of DPPH powder is added into a 100mL volumetric flask wrapped by tinfoil, and absolute ethyl alcohol is used for fixing the volume to the scale, so that a 0.2mmol/LDPPH solution is obtained. Taking 2mL to 10mL of the radix puerariae and rhizoma polygonati composite solution, adding 2mL of 0.2mmol/L DPPH solution, fully fusing, and carrying out dark reaction at 37 ℃ for 30 min. The absorbance at 517nm was measured and recorded as A 1 . Using 70% ethanol as reference solution instead of DPPH solution, adding sample solution, mixing, and dark reacting at 37 deg.C for 30 min. The absorbance at 517nm was measured and recorded as A 0 . The sample solution was replaced with absolute ethanol, fully fused, and dark reacted at 37 ℃ for 30 min. The absorbance at 517nm was measured and recorded as A 2 . And (4) carrying out parallel measurement on each solution to be measured for three times to obtain an average value.
DPPH clearance was 88.41%, 89.10%, 86.5% for samples 1, 2, and 3, respectively, indicating that the samples all had significant free radical scavenging ability.
2. Research on hydroxyl radical clearance rate of radix puerariae and rhizoma polygonati compound
0.1668g of ferrous sulfate hexahydrate is added into a 100mL volumetric flask, the volume is fixed by distilled water, and a 6mmol/L ferrous sulfate solution is prepared. 0.0839g of salicylic acid is added into a 100mL volumetric flask, absolute ethyl alcohol is used for constant volume, and 6mmol/L of salicylic acid ethanol solution is prepared. 0.06mL of 30% hydrogen peroxide solution was added to a 100mL volumetric flask, and a volume of the solution was determined by distilled water to obtain a 6mmol/L hydrogen peroxide solution. Adding 2mL of radix Puerariae and rhizoma Polygonati composite solution into 10mL of test tube with plug, adding 2mL of ferrous sulfate solution and 2mL of hydrogen peroxide solution, shaking, reacting in dark for 12min, adding 2mL of salicylic acid ethanol solution, reacting in dark for 30min, measuring absorbance at 510nm, and recording as A 1 . The compound solution of radix puerariae and rhizoma polygonati is replaced by distilled water as a reference solutionThe absorbance of the solution was measured and recorded as A 2 . Replacing salicylic acid solution with distilled water as blank control group, measuring absorbance, and recording as A 0 . Each solution to be measured is measured in parallel for three times and an average value is obtained.
The hydroxyl radical scavenging rates of the sample 1, the sample 2 and the sample 3 reach 71.93%, 79.62% and 75.38% respectively, which shows that the samples all show better hydroxyl radical scavenging capability, and the activity of the sample 2 is higher than that of the sample 1 and the sample 3.
3. Research on ABTS free radical clearance rate of radix puerariae and rhizoma polygonati compound
0.0461g of potassium persulfate powder was put into a 50mL volumetric flask, and distilled water was added thereto to set the volume to the mark, whereby a 3.41mmol/L potassium persulfate solution was obtained. 0.0165g of ABTS powder is added into a 50mL volumetric flask, and distilled water is added to the volumetric flask to reach the constant volume to the scale mark, so that 6.02mmol/LABTS solution is obtained. Mixing the two solutions 50mL each, wrapping the volumetric flask with tinfoil, and standing in shade for 12 h. Adding 2.5mL of radix Puerariae polyphenol extract solution or 2.5mL of ascorbic acid solution into a test tube, adding 2mL of LABTS free radical solution, reacting in dark and shade for 6min, measuring absorbance of radix Puerariae polyphenol extract solution or ascorbic acid solution at 734nm with ultraviolet spectrophotometer, and recording A 1 . Replacing radix Puerariae polyphenol extract solution or ascorbic acid solution with distilled water, measuring absorbance of the solution at 734nm with ultraviolet spectrophotometer, and recording A 2 . Replacing ABTS solution with distilled water, measuring absorbance of radix Puerariae polyphenol extract solution or ascorbic acid solution at 734nm with ultraviolet spectrophotometer, and recording A 0 . And (4) carrying out parallel measurement on each solution to be measured for three times to obtain an average value.
The ABTS free radical scavenging rates of sample 1, sample 2 and sample 3 were 87.61%, 89.68% and 88.97%, respectively, indicating that all 3 samples had excellent free radical scavenging ability.
Example 3
The invention preliminarily determines the main raw materials for preparing the kudzu root and polygonatum sibiricum compound solid beverage: radix puerariae is used as a monarch, rhizoma polygonati is used as a minister, liquorice is added to prepare various medicines, and the radix puerariae and rhizoma polygonati compound beverage is proved to have better antioxidant function activity based on the free radical clearance rate through DPPH free radical, hydroxyl free radical and ABTS free matrix external antioxidant activity evaluation.
TABLE-clearance of composite beverages for DPPH free radicals
Clearance rate of epi-di compound beverage for hydroxyl radical
Clearance rate of Epimedium complex beverage for ABTS free radical
Claims (10)
1. A preparation method of a kudzu root and polygonatum sibiricum compound solid beverage with antioxidant activity is characterized by comprising the following steps: the method comprises the following steps:
(1) crude extraction of pueraria polyphenol:
a1, selecting materials and cleaning: cleaning radix Puerariae, and cutting into pieces;
a2, crushing: pulverizing radix Puerariae, and sieving to obtain coarse powder;
a3, extraction: adding radix Puerariae powder into ethanol to desired volume, and performing ultrasonic treatment;
a4, filtering: centrifuging and filtering the extracting solution, and taking the supernatant for later use;
a5, extraction: adding ethanol into the radix Puerariae precipitate, extracting, filtering, repeatedly extracting for 2-3 times, and mixing extractive solutions;
a6, suction filtration: filtering the mixed supernatant, and placing in a beaker for later use;
(2) extracting and separating kudzu root polyphenol:
b1, extraction: adding radix Puerariae powder into ethanol, and performing ultrasonic treatment;
b2, suction filtration: filtering the extract, and placing in a volumetric flask for later use;
b3, suction filtration: placing radix Puerariae extract in conical flask, adding macroporous adsorbent resin, stirring, standing, and vacuum filtering;
b4, suction filtration: adding the filter residue into ethanol, shaking and filtering;
b5, concentrating: putting the filtrate into a round-bottom flask, and concentrating by using a rotary evaporator;
b6, freeze drying: freeze-drying the concentrated liquid for later use;
(3) crude extraction of polygonatum sibiricum polysaccharides
C1, drying: spreading rhizoma Polygonati on a culture dish, and placing in a drying oven;
c2, crushing: pulverizing rhizoma Polygonati into powder;
c3, leaching: adding rhizoma Polygonati powder into complex enzyme, performing enzymolysis, water bath, heating, and reacting;
c4, water bath: carrying out water bath reaction, and continuing the reaction after the temperature is increased;
c5, filtering: subpackaging the cooled solution, centrifuging, and mixing the supernatants;
c6, concentration: concentrating the supernatant with rotary evaporator;
(4) crude extract of licorice
D1, crushing: pulverizing Glycyrrhrizae radix pieces in a pulverizer, and sieving.
2. The kudzu root and rhizoma polygonati compound solid beverage with antioxidant activity prepared by the method of claim 1 is characterized in that: comprises the following raw materials in percentage by weight: 26.74g of kudzu root polyphenol extract, 50mL of polygonatum polysaccharide extract and 49.10g of licorice powder.
3. The preparation method of the kudzu root and polygonatum sibiricum compound solid beverage with antioxidant activity according to claim 1, which is characterized by comprising the following steps: extracting A3 in the step (1): adding 55.0g of kudzu root powder into a 1000mL beaker, fixing the volume to a scale with 70% ethanol, and carrying out ultrasonic treatment for one hour; extraction of A5: extracting the precipitate with 70% ethanol for 2-3 times.
4. The preparation method of the pueraria and polygonatum sibiricum compound solid beverage with antioxidant activity according to claim 1, which is characterized in that: extracting B1 in the step (2): adding 50g of sieved radix puerariae powder and 300mL of 70% ethanol into a 500mL beaker, and performing ultrasonic treatment at 60 ℃ for 1 h; suction filtration of B3 in the step (2): 50mL of radix puerariae extract is added into a 100mL conical flask, 5g of pretreated macroporous adsorption resin is added according to the solid-to-liquid ratio of 1: 10 (g: mL), the mixture is stirred at room temperature, placed for 2h and filtered.
5. The preparation method of the kudzu root and polygonatum sibiricum compound solid beverage with antioxidant activity according to claim 1, which is characterized by comprising the following steps: suction filtration of B4 in the step (2): adding 50mL 70% ethanol into the filter residue, shaking for 120min, and filtering.
6. The preparation method of the kudzu root and polygonatum sibiricum compound solid beverage with antioxidant activity according to claim 1, which is characterized by comprising the following steps: freeze-drying of B6 in the step (2): freeze drying at-20 deg.C.
7. The preparation method of the kudzu root and polygonatum sibiricum compound solid beverage with antioxidant activity according to claim 1, which is characterized by comprising the following steps: drying of C1 in the step (3): 50g of rhizoma polygonati are spread in a culture dish and placed in an oven at 40 ℃ for 48 hours.
8. The preparation method of the kudzu root and polygonatum sibiricum compound solid beverage with antioxidant activity according to claim 1, which is characterized by comprising the following steps: leaching of C3 in the step (3): 10g of sealwort powder and 200mg of complex enzyme are added into a 500mL flask, and the complex enzyme is pectinase and cellulose.
9. The preparation method of the kudzu root and polygonatum sibiricum compound solid beverage with antioxidant activity according to claim 1, which is characterized by comprising the following steps: the water bath of C4 in the step (3): the reaction was carried out in a water bath at 52 ℃ for 1h, and then the temperature was raised to 80 ℃ for 1 h.
10. The preparation method of the pueraria and polygonatum sibiricum compound solid beverage with antioxidant activity according to claim 1, which is characterized in that: filtering of C5 in the step (3): after cooling, the supernatant was placed in 4 centrifuge cups, centrifuged at 8000r/min, and the supernatant was mixed.
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