CN111788304A - 基因工程噬菌体 - Google Patents

基因工程噬菌体 Download PDF

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CN111788304A
CN111788304A CN201980009329.5A CN201980009329A CN111788304A CN 111788304 A CN111788304 A CN 111788304A CN 201980009329 A CN201980009329 A CN 201980009329A CN 111788304 A CN111788304 A CN 111788304A
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史蒂文·特里奥特
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Abstract

公开了一种将噬菌体工程化的方法，所述方法包括：鉴定仅有一个黏附基因的噬菌体；分离所述噬菌体；从所述噬菌体的基因组中去除所述黏附基因；和所述噬菌体的基因组中插入非天然黏附基因，其中所述非天然黏附基因特异性用于黏附于所选择的细菌。还公开了一种突变体噬菌体，其包含编码第一特异性黏附基因的异源核酸序列，第一特异性黏附基因不同于所失活的黏附基因并对所选择的细菌具有特异性。在另一实施方式中，公开了一种消除微生物污染物的方法，所述方法包括：获得由突变体噬菌体产生的一种后多种裂解酶；在无需事先用噬菌体对细菌污染物进行感染的情况下，将所述一种或多种裂解酶施加于细菌污染物，以消除所述细菌污染物。

Description

基因工程噬菌体
技术领域
人、动物和植物的细菌感染的预防、诊断和治疗。
背景技术
细菌是单细胞的生物实体，大多数对人无害，不同类型中有不到百分之一会使人患病。许多细菌对人类都是有益的，例如那些有助于消化食物、破坏致病细胞和提供所需维生素的细菌。
传染性细菌(有害的那百分之一)会导致人和动物患病。它们在体内快速繁殖并产生可导致组织损伤和疾病的有毒蛋白。
噬菌体(也称为细菌噬菌体)由Ernest Hankin于1896年发现，并被用作对抗霍乱的抗菌剂。这些细菌特异性的病毒可以感染并破坏细菌细胞。
噬菌体由包裹着DNA或RNA基因组的蛋白组成。通过将其病毒遗传物质(DNA或RNA)注入到宿主细胞中，噬菌体可以在细菌内复制，从而有效地接管细胞的功能，用以产生子代噬菌体，从而导致细胞壁破裂和随后的细菌细胞死亡。
直到1930年代，噬菌体一直被用作抗菌剂。然而，发现细菌自然地产生对噬菌体的抗性。随着化学抗生素的引入，人们放弃了使用噬菌体。
尽管抗生素是通常的治疗手段，但赋予抗生素抗性的细菌突变在全世界的致病性细菌中变得越来越普遍。例如，耐甲氧西林的金黄色葡萄球菌(MRSA)细菌是一种越来越常见的感染形式，通常通过医院内传播而获得。使用常规抗生素很难治愈MRSA感染。
噬菌体能够对引起疾病的细菌物种非常具有特异性。大多数噬菌体具有使其能够结合目标细菌表面特定分子的结构。
噬菌体的关键优势在于它们能够消除对抗生素具有抗药性的细菌，而无需使人、动物和环境暴露于毒性越来越大的抗生素、或有害或刺激性的化学品(例如，参见Intralytix的美国专利6,699,701号)。
在自然环境中，可以按照成对关系从特定细菌生长的环境中分离出噬菌体，例如从污水或粪便中进行分离。已经创建了不同类型的天然噬菌体的储存库，以提供对可治疗由特定细菌物种造成的难治性感染的噬菌体。
使用噬菌体的一个问题是，患者自体通常会对噬菌体产生免疫反应，并从血液中清除噬菌体。美国专利No.5,660,812号、美国专利No.5,688,501号、美国专利5,811,093号和美国专利5,766,892号均显示了选择或产生(利用突变)噬菌体以改善待在治疗患者血液中噬菌体半衰期的方法。
与先前使用噬菌体消灭或治疗细菌污染或疾病有关的另一个问题是细菌可变得对噬菌体具有抗性。细菌中例如原噬菌体的存在可阻断来自感染性噬菌体的基因表达，从而阻止了该感染性噬菌体的复制并阻止了对细菌的裂解和杀死。原噬菌体还可能引起对进入的噬菌体DNA的破坏。
这在以前意味着要么噬菌体需要与细菌匹配(通常需要对细菌进行复杂的遗传分析)，要么需要将多种不同的噬菌体组合使用。WO 03/080823中公开了不同噬菌体的组的生产，例如衍生自温和噬菌体的vir突变体的组。
当前，仅存在天然的噬菌体、和已经被突变并针对某些细菌进行特异性选择的天然的噬菌体(参见，例如，Intralytix的美国专利8,685,697号)。
发明内容
本发明是一种模板或平台技术，用于产生定制化基因修饰的噬菌体，其靶向并破坏在人、动物和农作物中以及在医疗保健或食品加工设施表面上发现的特定细菌生物。
可以使用此模板技术开发特定的产品，例如用于MRSA的消毒喷雾剂、防止在动物饲料中使用抗生素的食品添加剂、和对人的细菌感染的治疗。因此，本发明涵盖经基因修饰的噬菌体，并且包括衍生自噬菌体的基因产物，其用于利用噬菌体进行治疗和/或去除细菌感染。
根据一个方面，公开了一种操纵病毒基因组以在病毒的生命周期中引起功能改变的方法。
在一个实施方式中，本发明提供了一种将噬菌体工程化的方法，所述方法包括：
-鉴定仅有一个黏附基因的噬菌体；
-分离所述噬菌体；
-从所述噬菌体的基因组中去除所述黏附基因；和
-在所述噬菌体的基因组中插入非天然黏附基因，其中，所述非天然黏附基因特异性用于黏附于所选择的细菌。
在另一实施方式中，本发明提供了一种将噬菌体工程化的方法，所述方法包括：
-分离噬菌体；
-从所述噬菌体的基因组中去除任何黏附基因；
-插入第一独特开放阅读框，第一独特开放阅读框编码一个或多个黏附基因，并插入第二独特开放阅读框，第二独特开放阅读框编码一个或多个可用于克服细菌防御的基因；
-在所述第一开放阅读框中插入非天然黏附基因，其中，所述非天然黏附基因特异性用于黏附于所选择的细菌。所述一个或多个可用于克服细菌防御的基因是细胞内溶素、生物膜减少剂、糖萼穿透剂或其任何组合。
在另一实施方式中，本发明提供了一种将噬菌体工程化的方法，所述方法包括：
-分离噬菌体；
-从所述噬菌体的基因组中去除任何黏附基因；
-在所述基因组中插入多限制性酶盒；和
-在所述盒中插入非天然黏附基因，其中，所述非天然黏附基因特异性用于黏附于所选择的细菌。
在另一实施方式中，本发明提供了一种将噬菌体工程化的方法，所述方法包括：
-分离噬菌体；
-从所述噬菌体的基因组中去除所有天然黏附基因；和
-在所述噬菌体的基因组中插入非天然黏附基因；
其中，所述非天然黏附基因特异性用于黏附于所选择的细菌。
在另一实施方式中，本发明提供了一种使噬菌体生长的方法，所述方法包括：
-制备包含酵母和酵母营养物的酵母培养物；
-用噬菌体感染所述酵母；
-通过菌落-PCR筛选所述酵母中的阳性转化子。
所述噬菌体可包含非天然黏附基因，其中所述非天然黏附基因特异性用于黏附于所选择的细菌。噬菌体可不具有天然黏附基因。噬菌体可具有裂解性。非天然黏附基因对致病性/非致病性细菌具有特异性。噬菌体可用于清洁、治疗或预防细菌污染。
本发明还教导了用于诊断特定细菌存在或不存在的噬菌体。
本发明还教导了一种生产突变体噬菌体的方法，所述方法包括使选定的噬菌体的黏附基因失活，所述选定的噬菌体分离自来自环境的噬菌体；在选定的噬菌体中插入第一异源核酸序列，第一异源核酸序列包含编码第一特异性黏附基因的第一开放阅读框，第一特异性黏附基因不同于所失活的黏附基因并对所选择的细菌具有特异性，从而产生突变体噬菌体。可以将第二异源核酸序列插入到编码可用于克服细菌防御的基因的第二开放阅读框中。用于克服细菌防御的基因可以是生物膜降解基因、糖萼降解基因、编码抗菌蛋白的基因、和破坏细菌壁的酶的基因，从而产生突变体噬菌体。失活步骤可以使来自选定的噬菌体的所有黏附基因失活。
本发明还教导了一种噬菌体，其是裂解性噬菌体、具有小的基因组尺寸的细菌噬菌体、或具有裂解革兰氏阴性和革兰氏阳性细菌的结构和功能基因的噬菌体，或其任何组合。
本发明还教导了用于对表面进行消毒或去污的抗微生物组合物。
本发明还教导了一种消除微生物污染的方法，该方法包括：获得由突变体噬菌体产生的一种或更多种裂解酶；在无需事先用噬菌体对细菌污染进行感染的情况下，将一种或更多种裂解酶施用于细菌污染，以可消除细菌污染。
附图说明
图1示出了根据本发明的实施方式的噬菌体工程化平台的概况。
图2示出了根据本发明的实施方式的使用无细胞克隆方法产生突变体噬菌体的方法的概况。
图3示出了根据本发明的实施方式的使用酵母株产生突变体噬菌体的方法的概况。
图4是从基因模板中复活后针对大肠杆菌DH5α的pp8滴定的琼脂糖板。将噬菌体点涂在大肠杆菌的菌苔上。确定分离物1的噬菌体的浓度为每10μl108单位，分离物2的噬菌体的浓度为每10μl106单位。
图5示出了根据本发明的实施方式的所公开的突变体噬菌体的整个基因组的示意图。
图6示出了根据本发明的实施方式的PP8的整个基因组的核苷酸序列和其中编码的蛋白以及限制性核酸内切酶位点。
图7是根据本发明的实施方式的具有注释的PP8分子和蛋白的详细描述。
图8是利用特异性去除插入物的酶进行的PP8 DNA消化的凝胶电泳照片。EcoRI用于ORF1和ORF2，并且TspRI用于ORF 3和ORF 4，其中泳道1：1kb DNA梯状标记(NEB)，泳道2：空，泳道3：未消化的PP8 DNA，泳道4：消化的PP8 ORF1插入SP5黏附基因(46090)条带尺寸1.1kb，泳道5：消化的PP8 ORF2插入内溶基因(73195)条带尺寸2.1，泳道6：消化的PP8 ORF3插入SP6黏附基因(19991)条带尺寸1.2kb，泳道7：消化的PP8 ORF4插入内溶基因(60431)条带尺寸2.1。
图9a–显示凝胶电泳照片，其中泳道1：1kb DNA梯状标记(NEB)，2：空，3：提取的噬菌体基因组对照，4：细菌对照(假-噬菌体感染)，5-7：纯化的具有潜在整合的细菌菌落。预期条带尺寸：554个碱基。
图9b–是凝胶电泳照片，其中泳道1：提取的噬菌体基因组对照，2：细菌对照(假-噬菌体感染)，3-5：纯化的具有潜在整合的细菌菌落。预期条带尺寸：613个碱基。
图10示出了根据本发明的实施方式的公开的用于修饰结合位点的方法的概况。
图11显示了MRSA噬菌体治疗实验的结果，该实验为对于MRSA患者样品1-6的噬菌体PP8(SR5)插入裂解。浓度为107的噬菌体被用于制作6个MRSA阳性患者样品的致死曲线。这些样品被称为患者1-6。
图12显示了针对金黄色葡萄球菌的PP8/SP5的滴定。将噬菌体点涂在金黄色葡萄球菌的菌苔上。浓度确定为每10μl有105个噬菌体单位。
图13显示了针对金黄色葡萄球菌的PP8/SP6的滴定。将噬菌体点涂在金黄色葡萄球菌的菌苔上。浓度确定为每10μl有108个噬菌体单位。
图14显示了新的MRSA噬菌体治疗的结果，该实验为MRSA患者样品1-6的PP8(SR5，SR6)插入体致死曲线。使用浓度为105的噬菌体来制作6个MRSA阳性患者样品的致死曲线。在浓度为106的条件下测试患者样品的生存力。
图15是显示PP8 SP5/SP6细菌挑战试验的照片。将噬菌体PP8SP5/SP6倾倒在琼脂糖板上。测试细菌菌株的裂解。50)大肠杆菌O9，51)大肠杆菌O1，52)大肠杆菌O28，53)大肠杆菌DH5α，54)肠炎沙门氏菌，55)单增细胞李斯特菌，56)杜兰肠球菌，57-61)分别为MRSA患者样品1-5。
具体实施方式
除非另外指出，否则本公开的方法和技术通常根据本领域公知的常规方法来执行，并且如本说明书通篇所引用和讨论的各种一般性和更具体的参考文献中所述。
术语“多肽”、“肽”和“蛋白”在本文中通常可互换使用，以指代氨基酸残基的聚合物。氨基酸在本文中可以用它们通常已知的三个字母符号或IUPAC-IUB生化命名委员会推荐的单字母符号来指代。每种蛋白或多肽可具有独特的功能。本发明包括多肽及其功能片段，以及具有相同生物学功能或活性的突变体和变体。
在一些实施方式中，如果聚合分子(例如，多肽序列或核酸序列)的序列具有至少25％、至少30％、至少35％、至少40％、至少45％、至少50％、至少55％、至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％、至少95％或至少99％的同一性，则该聚合分子被认为彼此“同源”。
在一些实施方式中，核酸序列的片段是开放阅读框序列的片段。在一些实施方式中，这样的片段编码由开放阅读框核苷酸序列编码的蛋白的多肽片段(如本文所定义)。
如本文所用，术语“核酸片段”是指具有缺失的核酸序列。在一些实施方式中，核酸序列的片段是开放阅读框序列的片段。在一些实施方式中，这样的片段编码由开放阅读框核苷酸序列编码的蛋白的多肽片段(如本文所定义)。
术语“构建体”是指与启动子和/或其他调节序列可操作连接的编码蛋白的核酸序列。
术语“基因组序列”是指具有不连续的开放阅读框的序列，其中内含子中断了蛋白编码区。
如本文所用，当在指定核酸的上下文中使用时，术语“使编码”、“编码”或“被编码”(“encoding”、“coding”或“encoded”)是指核酸包含指导核苷酸序列翻译成指定蛋白的必要信息。通过密码子的使用来指定编码蛋白的信息。编码蛋白的核酸可以在核酸的翻译区内包含非翻译序列(例如，内含子)，或者可以缺少这样的中间非翻译序列(例如，在cDNA中)。
在核酸序列的上下文中，术语“序列同一性百分比”或“同一”是指两个序列中的残基在比对以获得最大对应性时是相同的。例如，可以使用计算机程序BLAST(Altschul等,J.Mol.Biol.215：403-410(1990)；Gish和States,Nature Genet.3：266-272(1993)比较多核苷酸序列。
当提及核酸或其片段时，术语“基本同源性”或“基本相似性”表示当与另一种核酸(或其互补链)以适当的核苷酸插入或缺失进行最佳比对时，通过任何公知的序列同一性算法(例如上述BLAST)测定，有至少约70％、80％、85％或至少约90％，或至少约95％、96％、97％、98％或99％的核苷酸碱基存在核苷酸序列同一性。
如本文所用，“异源核酸序列”是通常不存在的位于基因组中位置的任何序列。在一些实施方式中，异源核酸序列是天然噬菌体序列，不过来自不同噬菌体。
特定的核酸序列还涵盖其保守修饰的变体(例如简并密码子取代)和互补序列，以及明确指出的序列。因此，本文公开的编码蛋白序列的核酸序列也涵盖本文所述的其修饰变体。本发明的基本上相似的核酸片段的特征还可在于它们编码的氨基酸序列与本文公开的氨基酸序列的同一性百分比(根据本领域技术人员通常采用的算法所确定)。
“源噬菌体”是从自然或人工环境中分离的噬菌体，未经基因工程改造。“突变体噬菌体”是包含已经通过将异源核酸序列插入到基因组中而进行遗传修饰的基因组的噬菌体，或该噬菌体的基因组。在一些实施方式中，通过重组DNA技术修饰源噬菌体的基因组，以将异源核酸序列在限定的位点引入基因组。
“可操作地连接”或“可操作连接”的表达控制序列是指这样的连接：其中表达控制序列与目的编码序列邻接以控制目的编码序列的表达，以及反式作用的表达控制序列，或以一定距离控制编码序列的表达。
“编码序列”或“开放阅读框”是编码多肽或蛋白的核苷酸序列。编码序列的末端是起始密码子和终止密码子。本公开还包括编码蛋白的天然、分离或重组的核酸序列，以及包含该蛋白的编码序列的载体和/或(宿主)细胞。
本发明还涵盖所公开的核苷酸序列的片段和变体以及由其编码的蛋白。“片段”意图指核苷酸序列的一部分或氨基酸序列的一部分，以及因此指由其编码的蛋白。核苷酸序列的片段可以编码保留天然蛋白的生物学活性的蛋白片段。因此，本公开涉及任何核酸片段，其包含编码其所编码的全部或大部分氨基酸序列的核苷酸序列。
本技术使用合成生物学来产生能够结合特定细菌菌株的噬菌体。由于噬菌体必须黏附在宿主细菌细胞上以启动对细菌的感染，因此病毒DNA或RNA中的基因选择或操作可以定义结合特征，从而使宿主细胞的范围超出了自然配对关系。根据一个实施方式，所公开的噬菌体具有一些特征，包括以下特征。
噬菌体安全、无腐蚀和无毒。可以对噬菌体进行工程化，以使其不影响有用的细菌、动物或人细胞。因此，不会像抗生素那样干扰食物链。
所述噬菌体是经过设计的，不是自然界发现的。因此，该技术适用于任何细菌感染。消除了不合需要的遗传成分。相反，分离特定细菌的天然噬菌体的现有方法就像为目标细菌“大海捞针”。
对噬菌体进行工程化，以避免靶细菌发生突变/适应，从而导致较高的杀灭率且无耐受性。因此，所述噬菌体具有优于已知噬菌体的功效。噬菌体还防止生物膜形成。
根据一个实施方式，平台是通用的。所公开的噬菌体可用于解决任何细菌问题。所公开的噬菌体可应用于人类健康(个性化药物、消毒剂和诊断)，例如在MRSA和VRE中；动物健康(畜牧医学、诊断)，例如用于治疗中间葡萄球菌的狗耳感染的滴耳剂；以及食品安全(对细菌污染产生清洁、检测)，例如大肠杆菌、空肠弯曲菌、沙门氏菌和李斯特菌。
根据一个实施方式，噬菌体不仅可以用于治疗抗药性细菌感染，而且可以用于预防环境和食物中可能对人和动物健康产生负面影响的细菌污染。
例如，噬菌体可用于人类健康。耐甲氧西林的金黄色葡萄球菌(MRSA)细菌是一种越来越常见的医院获得性感染，通常通过接触被污染的表面而获得。对于具有已确认MRSA问题的设施，该产品可用于彻底清洁表面并减少新感染的发生。根据实施方式，提供了一种多菌株MRSA特异性消毒清洁剂，其可用于医院的多孔和无孔表面，包括床、窗帘、桌、椅、诊断和监测设备、以及医疗器械。
根据实施方式，所公开的噬菌体可以用于减少或消除使人和/或动物致病的任何细菌和/或抗性细菌。在某些方面，使用这种消毒剂相对于诸如漂白剂等常用消毒剂具有多个优点。首先，噬菌体比常规方法更有效地破坏细菌。其次，噬菌体可以留在表面上存活约24小时，以破坏新的细菌污染事件。第三，与漂白剂不同，噬菌体不会留下腐蚀性残留物，因此不会伤害仪器、织物和皮肤。第四，与清洁液不同，针对有害细菌定制的噬菌体是无毒的。
噬菌体也可用于动物健康治疗。例如，专门设计噬菌体替代常用的抗生素来应对鸡的细菌感染，从而产生了无抗生素的鸡，这在当今市场上有商业利益。这种治疗还有助于减少数量增长的由于细菌突变和进化为不受抗生素影响而发生的耐抗生素感染。
噬菌体也可用于食品安全。例如，可以将噬菌体清洁喷雾剂施用到农作物上，以防止植物栽培或收获过程中的细菌污染，诸如草莓的大肠杆菌污染所导致的食源性疾病。
利用模板技术产生具有各种特异性结合域(从而选择宿主范围)的噬菌体。该技术可提供高浓度的噬菌体。
在一些实施方式中，源自噬菌体的基因产物可用于“自外裂解”，由此可以使细菌在不必被感染的情况下被消除。
根据实施方式，提供了一种在无需事先用噬菌体对细菌污染物进行感染的情况下消除细菌污染物的方法，该方法包括获得一种或跟多种由所公开的噬菌体产生的裂解酶；将一种或更多种裂解酶应用于细菌污染物以消除细菌污染物。
噬菌体或细菌噬菌体被定义为感染细菌的病毒。噬菌体对其相应的宿主细菌具有高度特异性。为了感染细菌，噬菌体黏附于在细菌表面上的特定受体。这种黏附决定了每个噬菌体的宿主范围，通常仅限于一些属、种甚至亚种的细菌。这种噬菌体特异性可以为临床医生、实验室技术人员、该领域的技术人员、以及消费者提供通过利用这种噬菌体特性来鉴定(检测或诊断)特定类型细菌的能力。
噬菌体经历两种类型的自然生命周期或病毒繁殖方法，称为裂解周期和溶原性周期。在裂解周期中，病毒体复制后宿主细胞将被破坏并死亡。相比之下，溶原性周期不会导致宿主细胞立即裂解和随后的宿主细胞死亡；相反，噬菌体基因组与宿主DNA整合，或将自身建立为质粒，并随生物体的基因组一起复制。内源噬菌体保持休眠状态，直到宿主暴露于特定条件下(例如，压力)，此时噬菌体可以被激活，从而开始繁殖周期，导致宿主细胞裂解。
内溶素在噬菌体裂解周期的最后阶段从其宿主体内产生，大部分释放到周质空间中(Borysowski等，2006)。从那里开始，内溶素裂解肽聚糖中的共价键以释放病毒子代(Fischetti，2008)。在内溶素亚组中有五类：酰胺酶、内肽酶、胞壁质酶、氨基葡萄糖苷酶和转糖基酶(Gasset，2010)。
根据实施方式，提供了来自细菌病毒的裂解酶或抗生酶在对抗抗微生物剂抗性中的应用。抗生酶被定义为降解细菌细胞壁的蛋白，这意味着它不会受到噬菌体蛋白的影响(Borysowski和Gorski，2010)。术语“抗生酶”首先在论文‘Prevention and eliminationof upper respiratory colonization of mice by group A streptococci by usingbacteriophage lytic enzyme’(Nelson等，2001)中提出。噬菌体裂解酶具有特异性。噬菌体衍生的裂解酶及其对致病性细菌菌株中发现的细胞壁某些成分有破坏性活性，但对动物的天然微生物群却没有破坏性(Gasset.2010)。两个实例包括C型的链球菌溶素，其可有效溶解A型的链球菌，但对正常的口腔链球菌没有影响(Fischetti，2006)。通过使用通常在致病性革兰氏阴性大肠杆菌中表达的外膜蛋白FyuA，可以得到更相关的实例。FyuA结合域与T4溶菌酶的融合导致融合物从外膜转移到周质空间，在此溶菌酶能够破坏细菌细胞壁的稳定性(Lukacik等，2013)
根据一个实施方式，公开了一种提供内溶素蛋白或多种内溶素蛋白的方法，其克服了采用完整噬菌体的问题。一种或多种内溶素特异性地从细胞内或细胞外靶向并降解细菌细胞壁(肽聚糖)，从而导致裂解。在各方面中，提供了一种方法，该方法从多种噬菌体中产生内溶素基因的各种克隆，并使用高通量筛选和评估内溶素克隆对抗一种或多种细菌例如大肠杆菌菌株、鼠伤寒沙门氏菌和空肠弯曲菌的成功性。
因此，该技术扩展了可以在有或没有感染的情况下以噬菌体或噬菌体基因产物处理的细菌菌株的数量。
噬菌体通过感染和杀死细菌而使自己倍增。在此过程中，细菌细胞壁成分随噬菌体一起释放。这些成分可能对人、动物和细菌有毒。因此，使用细菌大规模制备噬菌体需要使用刺激性有机化学物质的制造后处理，以将毒性降低至临床治疗可接受的水平。
因此，根据一个实施方式，提供了一种通过使用酵母菌株，例如乳酸克鲁维酵母和巴斯德毕赤酵母来生长所公开的在细菌大规模使用中的噬菌体的方法。所公开的方法避免了有毒终产物的释放。
产生突变体噬菌体
分离环境样品并进行充分表征，以确定符合某些标准的候选样品。优选地，从包括一个或多个以下特征的候选物中选择合适的源噬菌体：
-裂解性噬菌体；
-遗传上与已知噬菌体不同；和
-仅携带一个黏附基因。
根据实施方式，提供了一种遗传修饰一种或多种合适的源噬菌体的方法。
在一个实施方式中，源噬菌体包括一个黏附基因。在另一个实施方式中，源噬菌体包括多于一个黏附基因。
在一个实施方式中，该方法产生噬菌体平台，该噬菌体平台被配置为允许进一步交换一种或多种所需蛋白，例如黏附蛋白。
根据一个实施方式，操纵噬菌体基因组以改变病毒的生命周期，产生功能获得、功能丧失或用于病毒鉴定(报告基因)。总结如图1所示。
一方面，源噬菌体是裂解性噬菌体。一方面，源噬菌体是携带一个或多个黏附基因的裂解性噬菌体。在另一方面，源噬菌体是仅携带一个黏附基因的裂解性噬菌体。一方面，源噬菌体仅携带一个黏附基因。
根据一个实施方式，提供了一种生产突变体噬菌体的方法。一方面，该方法包括修饰源噬菌体的噬菌体结合位点，以使突变体噬菌体能够黏附于不同的血清型。在一个实施方式中，随后复活突变体噬菌体并确定新的结合域。
根据一个实施方式，工程化噬菌体仅包含裂解基因，其中任何和所有溶原基因已被去除以确保不会发生整合。
根据一个实施方式，提供了一种“无细胞克隆”的方法，以提供模板(或平台)技术，其允许修饰/插入/缺失病毒基因。该平台使用分离的环境样品通过构建突变体噬菌体(定义为由已知和未知遗传密码生成的噬菌体)而生成。
相对于已知的噬菌体类型，对来自环境样品的未知噬菌体类型进行测试基因对比，从而使我们能够分离出已知的基因类型。
在一个实施方式中，提供了一种突变体噬菌体，其中添加了感兴趣的基因，并且其中缺失了不合乎需要的基因。连同非编码区，突变体噬菌体是携带至少两个独特的开放阅读框(ORF)的遗传平台。
这些独特的ORF可用于添加感兴趣的基因。参考图2，将基因组互补物分成通过PCR扩增获得的与相邻片段有重叠部分的片段。将外源基因插入各自的片段内。采用同源重组方法，使用细菌细胞提取物将片段合并在一起，其中提取物包含必要的成分，以通过同源性将片段连接在一起成为一个连续的片段。通过无细胞翻译可从完全组装的基因组中拯救噬菌体。该方法涉及将选择的DNA与大肠杆菌的无毒细胞提取物以及氨基酸和能量混合在一起，提取物中的转录和翻译蛋白和酶驱动该DNA的表达，从而导致噬菌体的产生。
在各方面，突变体噬菌体是携带四个独特的开放阅读框(ORF)的遗传平台。
在一个实施方式中，第一ORF可用于插入用于细菌的黏附基因。一方面，黏附基因可以选自但不限于以下蛋白：
·肠杆菌科的DNA结合噬菌体蛋白(>CP007523.1：3585236-3586111沙门氏菌肠亚种，肠型鼠伤寒杆菌str.CDC 2011K-0870，完整基因组)SEQ ID No：125
·DNA结合噬菌体蛋白(>CP002910.1：3892390-3893265肺炎克雷伯菌KCTC 2242,完整基因组)SEQ ID No：126
·DNA结合蛋白(>CM000724.1：300852-301217蜡状芽孢杆菌BDRD-ST26染色体，全基因组鸟枪法测序)SEQ ID No：127
·噬菌体DNA结合转录调节物(>CP003678.1：c575894-575136肠杆菌阴沟亚种溶解型SDM,完整基因组)SEQ ID No：128
·噬菌体ssDNA结合蛋白(>CP009983.1：941901-942146副溶血性弧菌菌株FORC_008染色体2，完整序列)SEQ ID No：129
·DNA结合蛋白(>CM000749.1：288493-288840苏云金芽孢杆菌，T04001染色体，全基因组鸟枪法测序)SEQ ID No：130
·噬菌体核苷酸结合蛋白(>CP006620.1：c2486999-2486259粪肠球菌Aus0085,完整基因组)SEQ ID No：131
·DNA结合蛋白噬菌体P4(>AE005174.2：318190-318450大肠杆菌O157：H7str.EDL933基因组)SEQ ID No：132
·CP4-6原噬菌体；推定的DNA结合转录调节物(>HG738867.1：c269405-268512大肠杆菌str.K-12substr.MC4100完整基因组)SEQ ID No：133
·DNA结合蛋白(假伯克氏菌K96243染色体1，完整序列)SEQ ID No：134
·推定的DNA结合噬菌体蛋白(>AL590842.1：c1239408-1238512鼠疫耶尔森菌CO92完整基因组)SEQ ID No：135
·推定的DNA结合噬菌体蛋白(>AL590842.1：1235071-1235391鼠疫耶尔森菌CO92完整基因组)SEQ ID No：136
·推定的噬菌体相关DNA结合蛋白(>BX950851.1：4152092-4152508胡萝卜欧文氏菌亚种SCRI1043,完整基因组)SEQ ID No：137
在一个实施方式中，第二ORF用于插入编码可用于克服细菌宿主防御的蛋白的基因。
例如，第二ORF可以用于引入酶解功能来对抗细菌防御。一方面，第二ORF可用于添加细胞内溶素基因和/或生物膜降解基因。
在实施方式中，细胞内溶素基因选自：
·PP1噬菌体内溶素，SEQ ID No：138，其与大肠杆菌噬菌体B2相似：93％同一和100％查询覆盖率，登录号：MG581355；肠杆菌噬菌体JL1：92％同一和100％查询覆盖率，登录号：JX865427；志贺氏菌噬菌体EP23：91％同一和100％查询覆盖率，登录号：JN984867；苏达利菌噬菌体：91％同一和100％查询覆盖率，登录号：GQ502199；
·PP2噬菌体内溶素，SEQ ID No：139，其与大肠杆菌噬菌体phiLLS相似：99％同一和100％查询覆盖率，登录号：KY677846；沙门氏菌噬菌体Stp1：98％同一和100％查询覆盖率，登录号：KY775453；沙门氏菌噬菌体SP01：98％同一和100％查询覆盖率，登录号：KY114934；T5噬菌体样叉29：97％同一和100％查询覆盖率，登录号：MF431732；
·PP3噬菌体内溶素，SEQ ID No：140，其与肠杆菌噬菌体ATK48相似：99％同一和100％查询覆盖率，登录号：KT184310；志贺氏菌噬菌体SHSML-52-1：99％同一和100％查询覆盖率，登录号：KX130865；大肠杆菌噬菌体APCEc01：99％同一和100％查询覆盖率，登录号：KR422352.1；大肠杆菌O157分型噬菌体6：98％同一和100％查询覆盖率，登录号：KP869104；志贺氏菌噬菌体Shf125875：98％同一和100％查询覆盖率，登录号：KM407600；志贺氏菌噬菌体phi25-307：98％同一和100％查询覆盖率，登录号：MG589383；克雷伯氏菌噬菌体vB_Kpn_F48：73％同一和98％查询覆盖率，登录号：MG746602；
·PP7噬菌体内溶素，SEQ ID No：141，其与沙门氏菌噬菌体ST11相似：95％同一和100％查询覆盖率，登录号：MF370225；沙门氏菌噬菌体Meda：95％同一和100％查询覆盖率，登录号：MH586731；沙门氏菌噬菌体Si3：95％同一和100％查询覆盖率，登录号：KY626162；大肠杆菌噬菌体EC6：95％同一和100％查询覆盖率，登录号：JX560968；噬菌体Felix 01：95％同一和100％查询覆盖率，登录号：AF320576；肠杆菌噬菌体KhF2：94％同一和100％查询覆盖率，登录号：KT184314；沙门氏菌病毒VSe102：94％同一和100％查询覆盖率，登录号：MG251392；沙门氏菌噬菌体蘑菇：94％同一和100％查询覆盖率，登录号：KP143762；葡萄球菌噬菌体SA1：94％同一和100％查询覆盖率，登录号：GU169904；大肠杆菌O157分型噬菌体15：94％同一和100％查询覆盖率，登录号：KP869113；柠檬杆菌噬菌体Mijalis：83％同一和99％查询覆盖率，登录号：KY654690；志贺氏菌噬菌体Sf14：82％同一和99％查询覆盖率，登录号：MF327003；
·PP11噬菌体内溶素，SEQ ID No：142，其与肠杆菌噬菌体HK578相似：79％同一和97％查询覆盖率，登录号：JQ086375；大肠杆菌噬菌体Sloth：78％同一和97％查询覆盖率，登录号：KX534339；大肠杆菌噬菌体Envy：78％同一和97％查询覆盖率，登录号：KX534335；
·阴沟肠杆菌A1S1噬菌体内溶素SEQ ID No：143。
在一个实施方式中，生物膜降解基因和糖萼降解物选自：
Figure BDA0002593461260000161
例如，第二ORF可以用于引入模板中使用的抗菌蛋白以进行细菌裂解。一方面，示例蛋白是用于降解金黄色葡萄球菌(>ENA|JQ066320|JQ066320.1金黄色葡萄球菌菌株JP1Psmβ1(psmβ1)和Psmβ2(psmβ2)基因，完整的cds)的细菌细胞壁降解剂。SEQ ID No：144。
在其他方面，第二ORF可用于引入靶向细菌细胞壁中发现的关键连接化学键(酰胺、酯和糖酵解键)的酶。示例包括：
·M20家族肽酶[未培养细菌]，登录号AHZ45606(未培养细菌，>KF835382.1：c34024-32630未培养细菌克隆SZR5基因组序列)SEQ ID No：145
·脂解酶(未培养细菌登录号AHZ45613>KF835383.1：7038-8066未培养细菌克隆WZR9基因组序列)SEQ ID No：146
·肽酶M56([未培养细菌]登录号AHZ45657未培养细菌克隆WZR18基因组序列(>KF835385.1：c14123-13038未培养细菌克隆WZR18基因组序列)SEQ ID No：147；
·另一实例是未培养细菌克隆HOAb112C长链脂肪酸CoA连接酶基因([未培养细菌]DBSOURCE登录号KF955286.1)SEQ ID No：148；
·葛佬素样蛋白1的家蚕BmGloverin1 mRNA，完整登录号AB190863SEQ ID No：；149
·葛佬素样蛋白2的家蚕BmGloverin2 mRNA，完整登录号AB190864SEQ ID No：150；
·葛佬素样蛋白3的家蚕BmGloverin3 mRNA，登录号AB190865SEQID No：151；and
·葛佬素样蛋白4的家蚕BmGloverin3 mRNA，登录号AB190866SEQ ID No：152；
根据实施方式，提供了一种生产突变体噬菌体的方法，该方法包括使选定的噬菌体的至少一个黏附基因失活，所述选定的噬菌体可以分离自来自环境的噬菌体。该方法还包括将包含一个或多个黏附基因的一个或多个异源核酸序列插入选定的噬菌体中。一个或多个插入的黏附基因不同于所失活的天然黏附基因，并且由于其对所选择的细菌有特异性而被选择，以产生突变体噬菌体。在一些实施方式中，提供选择的黏附基因扩展了可能的宿主细胞(即细菌)的范围，超出了自然配对关系。
根据实施方式，提供了一种生产突变体噬菌体的方法，该方法包括使选定的噬菌体的至少一个黏附基因失活，所述选定的噬菌体可以分离自来自环境的噬菌体。该方法还包括将第一异源核酸序列插入选定的噬菌体中，第一异源核酸序列包含编码第一特异性黏附基因的第一开放阅读框。第一特异性黏附基因不同于所失活的黏附基因，并且由于其对所选择的细菌有特异性而被选择，以产生突变体噬菌体。
在另一实施方式中，该方法还包括将第二异源核酸序列插入第二开放阅读框中，编码可用于克服细菌防御的基因。在一些方面，用于克服细菌防御的基因可以是生物膜降解基因、糖萼降解基因、编码抗菌蛋白的基因、和破坏细菌壁的酶的基因，从而产生突变体噬菌体。在一个方面，第一开放阅读框还编码与第一特异性黏附基因不同的第二特异性黏附基因。
在一些实施方式中，该方法失活来自选定的噬菌体的所有黏附基因。在一些方面，失活的步骤包括在至少一种天然黏附基因中产生失活突变。在一些方面，失活突变是点突变。
根据实施方式，提供了一种用于对表面进行消毒或去污的抗微生物组合物。在一些方面，所述抗微生物组合物包含所公开的突变体噬菌体。
根据实施方式，提供了一种对怀疑含有细菌的表面去污的方法。在一些方面，细菌是感染性或非感染性细菌。该方法包括将包含所公开的突变体噬菌体的所公开的抗微生物组合物施加至表面。在各方面中，其量可有效地对至少基本上或所有污染细菌的表面进行去污。
在一些方面，该表面是生物表面(动物或植物)。
根据实施方式，提供了一种产生特异性突变体噬菌体基因产物的方法。在各方面中，提供了一种消除或基本上消除微生物污染物(感染性或非感染性细菌)的方法，该方法包括：获得由所公开的突变体噬菌体产生的一种或多种裂解酶，并施加所述一种或多种裂解酶至细菌污染物。在某些方面，所述消除在没有对微生物污染物进行事先噬菌体感染的情况下完成，因此导致自外裂解的结果。
在本说明书中提到的所有出版物和专利都通过引用并入本文，并入程度就好像每个单独的出版物或专利都被具体地和单独地指出通过引用并入一样。
出于说明和描述本发明的目的，已经给出了本发明的前述描述以及某些代表性实施方式和细节。并不旨在穷举本发明或将本发明限制为所公开的精确形式。对于本领域技术人员而言显而易见的是，可以在不背离本发明范围的情况下进行修改和变型。
例子
例1–噬菌体分离
收集并纯化来自下水道和废物、环境土壤和动物粪便的样品，以用于分离噬菌体。然后筛选所纯化的样品中是否存在针对特定细菌的噬菌体。根据Bonilla等(2016)和Bourdin等(2014)采用从水样中分离噬菌体的方案和方法；根据Sillankorva(2018)、Pausz等(2009)和Van Twest&Kropinski(2009)采用从固体和土壤样品中分离噬菌体的方案和方法。
使用无菌水将固体样品再水化至少1小时，以使噬菌体得以散播。然后将样品离心以去除固体物质和大颗粒，并收集上清液。然后将离心的环境样品和水样品进行进一步处理，并使用过滤器(0.2μM)进行纯化，以去除细菌和较小的有害颗粒。过滤后的样品可以使用滤管进一步浓缩，或者储存在4℃下以备将来使用。
然后使用琼脂覆盖菌斑测定技术(Kropinski等，2009)对过滤后的样品进行抗目标细菌菌株的测试。用过滤的环境样品和选择的细菌菌株接种液体琼脂覆盖物并混合。然后将其倒入琼脂培养板上(取决于细菌菌株)并使其硬化。然后将板温育过夜(条件取决于细菌菌株)，并在第二天观察针对所选菌株的噬菌斑。然后挑出含有噬菌体的菌斑，并通过3轮的随后噬菌斑测定覆盖物进行进一步处理，以纯化所选择的噬菌体。
使用上面概述的方法，收集了大量(数百个)EV样本，并测试了其对开发模板的适合性。对每个EV样品的功能和结构基因进行了表征，进行了整合测试(如下具体所述)。鉴定了具有低拷贝数溶原基因，以及可允许革兰氏阴性和革兰氏阳性裂解的结构和功能基因的候选噬菌体。对名为PP8的所选定的噬菌体进行测序，并如下文具体所述检查基因结构和功能。选择PP8，因为它具有所需的基因。尽管它也具有溶原性基因，但可以使用ORF替换将它们去除。
例2–平台开发
使用环境样品EV31/PP8，在噬菌体分离后，使用PureLink病毒DNA/RNA提取试剂盒纯化了基因组材料。扩增全长基因组(EV31/Full/F/pYESIL和EV31/Full/R/pYESIL，见序列EV31)，以与pYESIL蓝宝石载体具有30bp的同源性。使用Phusion高保真DNA聚合酶进行PCR扩增(修饰使用触地(touchdown)技术，使引物从69℃开始退火，每个循环下降0.5℃)。在琼脂糖凝胶上分离PCR产物，将条带切下、提取并组装。所得的构建体EV31pYES(未修饰)可用于EV31的基因修饰和基于噬菌体拯救的系统中突变功能的确定。
例3–全长基因组组装
简而言之，以下提供了一种对酵母(乳酸克鲁维酵母和巴斯德毕赤酵母)细胞基因操作的方法，以使其包括来自大肠杆菌噬菌体T7的T7 DNA(脱氧核糖核酸)依赖的RNA(核糖核酸)聚合酶转录，然后在酵母中表达噬菌体。还提供了一种对酵母(乳酸克鲁维酵母和巴斯德毕赤酵母)细胞基因操作的方法，以使其在酵母内包括来自细菌(大肠杆菌)和RNA(核糖核酸)聚合酶(P)的转录成分，然后在酵母表达噬菌体。
参见图3，将基因组互补物分成与通过PCR扩增获得的相邻片段有重叠部分的片段。将外源基因插入各自的片段内。通过同源重组方法将片段合并成全长基因组，并在酵母菌株毕赤酵母(Pichia pastoris)内将基于酵母的质粒(作为额外的PCR片段)与T7启动子结合在一起。在T7启动子控制下的稳定质粒在诱导含有T7 RNA聚合酶的巴斯德毕赤氏酵母后驱动噬菌体的复活，然后使用酶和机械手段裂解细胞以释放完全形成的噬菌体颗粒。
EV31pYes(未修饰)与pYESIL载体的同源重组是通过使用100ng的每种PCR产物实现的，并将其转到化学感受态的酵母细胞中。合并pYESIL载体(100ng)和EV31(100ng)。加入感受态酵母细胞并轻轻混合，然后加入600μl聚乙二醇(PEG)和醋酸锂(LiAc)溶液，然后轻轻混合。将混合物在30℃下温育30分钟，以10分钟的间隔倒置。温育后，立即加入35.5μl的二甲基亚砜(DMSO)，通过颠倒混合，并在42℃下热激20分钟(偶尔颠倒)。然后将试管以200-400xg离心5分钟，弃去上清液，并将细胞沉淀重悬于1ml无菌0.9％氯化钠(NaCl)中。通过将100μl平铺在选择性琼脂平板(不含色氨酸的培养基)上，并在30℃下温育3天，可以实现转化的可视化。菌落PCR筛选可以确定阳性转化子的存在。通过标准克隆技术实现同源重组，以使酿酒酵母菌株5150达到化学感受态。简而言之，使用Gietz和Schiestl 2007方案，从储备中产生单个酵母菌落的扩散板，并在30℃下温育过夜。第二天，刮取50μl当量的细胞，并在试管中用1ml无菌无核酸酶水洗涤，然后13,000xg旋转0.5分钟。将以下物质按顺序添加到细胞沉淀中：240μl PEG-3350(50％w/v)，36μl LiAc(1M)，50μl单链载体DNA(2mg/ml猪精子)和34μl质粒-无核酸酶水混合物(<lμg质粒)。将其轻轻涡旋混合以在42℃温育20-180分钟(时间取决于菌株)。对于EV31，使用了45分钟。转化后，将其旋转13,000xg 0.5分钟，然后去除上清液，并将沉淀重悬于1ml无菌无核酸酶水中。将混合物铺展到选择性培养基平板上，没有尿嘧啶的酵母合成的滴滤培养基，并在30℃下温育3-4天。使用菌落-PCR筛选进行克隆的验证。
图4显示了从遗传模板中复活的PP8的滴定。
图5显示了描述EV31/PP8基因位置的图形表示，并且图6中提供了整个基因组的详细核苷酸序列，显示了有义链(SEQ ID NO：1)、互补序列(SEQ ID NO：2)的反义链，和其中编码的蛋白的序列(SEQ ID NO：3-124)以及限制性核酸内切酶位点。图7显示了具有注释的EV31/PP8分子和蛋白的具体描述。
例4-通过菌落PCR进行克隆验证
按照以下方式进行阳性转化子(平板生长菌落)的筛选。将单个酵母菌落放入15μl裂解缓冲液中进行接种。在单独的试管中，转移5μl的每种混合物并保存在4℃下，以备用于阳性菌落的大规模培养。将剩余的10μl细胞悬液在95℃下煮5分钟，然后立即置于冰上，加入40μl无核酸酶的水并混合。在每个总体积为50μl的PCR反应中加入0.5μl的裂解物，并通过琼脂糖凝胶电泳使其可视化。PP8 DNA消化所得的凝胶如图8所示。
例5-独特ORF的开发
使用PP8模板，产生了突变体噬菌体。使用同源重组通过产生点突变来去除天然黏附蛋白。
基因中断14452-13316(尾蛋白)：
>PP8-F1F
ACAAATAGTGAAGAGATAAACCAGGTTGAGCAAG SEQ ID No：185
>PP8-尾-mut-R
TTGACGTTGAATCTGGAGTCGATAGGTGCGACAGGTTACCAATGG SEQ ID No：186
>PP8-尾-mut-F
GTCGCACCTATCGACTCCAGATTCAACGTCAAGGTCTCACC SEQ ID No：187
>PP8-F1R
TTCCAAGACGGATTCGAACCGTCACTAGTACAAGG SEQ ID No：188
基因中断14823-14446(尾蛋白)：
>PP8-F1F
ACAAATAGTGAAGAGATAAACCAGGTTGAGCAAG SEQ ID No：185
>PP8-hyp1-mut-R
TTAATGATGTTATCTCGATAACGTCGACATGGAGACTCAGTAAATGG SEQ ID No：189
>PP8-hyp1-mut-F
TCTCCATGTCGACGTTATCGAGATAACATCATTAAGGTTGTACC SEQ ID No：190
>PP8-F1RTTCCAAGACGGATTCGAACCGTCACTAGTACAAGG SEQ ID No：188
基因中断16522-17937(尾蛋白)：
>PP8-F1F
ACAAATAGTGAAGAGATAAACCAGGTTGAGCAAG SEQ ID No：185
>PP8-hyp2-mut-R
TTGAATAAACCGTTATCGCCTTCTTAAAGCAACCTGTATTGCGTTCTGC SEQ ID No：191
>PP8-hyp2-mut-F
TTGCTTTAAGAAGGCGATAACGGTTTATTCAACAAACCCTCATTTCATT G SEQ ID No：192
>PP8-F1R
TTCCAAGACGGATTCGAACCGTCACTAGTACAAGG SEQ ID No：188
基因中断34777-37020(尾蛋白)：
>PP8-F3F
TTCTTAAGGAGGGTTATGAATGTGTTATACAGG SEQ ID NO：158
>PP8-tape-mut-R
TCTGTGTAGTTCGGCCAACTGTAGTGTGCGAATGATGCAGCGAACATTC SEQ ID No：193
>PP8-tape-mut-F
TTCGCACACTACAGTTGGCCGAACTACACAGATACCATGAAGCAGTACTC SEQ ID No：194
>PP8-F3R
GTGGTAAGGTAAGGTATGGAAGGATGGCAGTAG SEQ ID NO：167
突变体噬菌体可以包括四个ORF：ORF 1位于46090位；ORF 2位于73195位；ORF 3位于19991位；ORF 4位于60431位。
使用针对ORF1(在46,090和46,091位核苷酸之间)的修饰引物EV31/0RF1/F和EV31/ORF1/R和针对ORF 2(在73,195和73,196位核苷酸之间)的EV31/ORF2/F和ORF2/R以及无细胞克隆生成了三个EV31突变体构建体。EV31(ORF1)、EV31(ORF2)和EV31(ORF1/2)。在ORF2的构建体中，首先从EV31中去除了天然结合域，并添加了多限制性酶盒。该盒随后用于添加新的细菌结合域。
使用限制性消化同时进行同源重组和插入。对于限制性消化，酶TspRI允许插入多克隆位点(MCS)。在一个实例中，ORF位于3ev31/pp8序列中的19991处。在此实例中，通过使用TspRI完成MCS的插入。一旦插入MCS后，可以通过使用MCS中的限制性酶切位点来实现所选择的黏附基因的插入。用于ORF3的MCS的实例：GCCGGCAGTGGATCCCCGGGGAAGATATTC SEQID NO：153。此MCS携带NaeI、TspRI、XmnI、SmaI的酶位点。用于将MCS添加19991位的引物是：EV31 ORF3引物f GCTACACTGCTGAGA SEQ ID NO：154；EV31 ORF3引物r TCTCAGCAGTGTAGCSEQ ID NO：155。
第四个ORF位于ev31/pp中的60431处。在此实例中，将通过使用TspRI完成MCS的插入。一旦插入MCS后，可以通过使用MCS中的限制性酶切位点来实现所选择的黏附基因的插入。用于将MCS添加到60431位的引物是：EV31 ORF4引物f CATCAGATGCTGG SEQ ID NO：156；EV31 ORF4引物r CCAGCATCTGATG SEQ ID NO：157。
例6–整合
对EV31/PP8基因组的分析显示，可能的溶源基因位于60351-62336。通过在60431(ORF4)位点产生ORF来突变基因。一旦溶原基因失活，我们就进行整合研究以确保不会发生整合。结果显示在图9a和9b中。
在促进整合的条件下，确认PP8缺乏整合能力。凝胶电泳照片确定了由噬菌体(噬菌体诱导对照)证明的整合事件，该整合事件通过对整个细菌细胞的聚合酶链反应(PCR)确定。如果将噬菌体遗传材料整合到纯化(无噬菌体颗粒)的细菌菌落中，则每个噬菌体的相应引物组将给出阳性PCR信号(右图；泳道5)。相反，PP8无法整合到细菌宿主细胞中，如照片中没有PP8序列阳性信号的情况所示(左图；泳道5-7)。
创造整合条件
在Luria-Bertani(LB)液体培养基中制备来自甘油原液的细菌宿主(大肠杆菌C)的新鲜过夜培养物。一旦饱和，将培养物在补充有2mM CaCl2的新鲜LB培养基中稀释(1：100)，并温育直至OD600为0.6。将在3mL熔化的软琼脂中的宿主(100μL大肠杆菌C)和噬菌体(100μL，感染复数为5)的混合物铺在事先干燥的LB琼脂平板上。过夜温育后，从每个平板中挑选三个菌落，重新划线到新鲜的LB-琼脂平板上，并温育过夜，进行三轮。将纯化的菌落(不含污染性噬菌体颗粒)接种到LB-2mM CaCl2培养基中，温育过夜。
分析潜在的整合事件
按照制造商的建议设置
Figure BDA0002593461260000251
DNA聚合酶(Promega)以及每种待评估噬菌体的引物的聚合酶链反应(PCR)主混合物。将每种过夜培养物中的5μL加入到各自的PCR反应中。改变循环条件，以包括在初始变性阶段使全细菌细胞煮沸(95℃持续10分钟)和59℃的退火温度。以5μL完成的PCR反应物进行1％琼脂糖凝胶电泳，染色并在紫外线(UV)下可视化。结果显示在图9a和9b中。
例7-ORF1插入SP5黏附蛋白(46,090和46,091之间)
使用PP8，我们利用PP8模板开发了MRSA特异性PP8结合噬菌体，我们去除了天然黏附基因，并利用同源重组在ORF 1位置(46,090和46,091之间)添加了黏附蛋白SP5。用于该同源重组的引物组是：
1.PP8片段(加粗)和SP5基因(下划线)在5’同源重组的引物组
>PP8-F3F
Figure BDA0002593461260000252
>PP8-SP5-R
Figure BDA0002593461260000253
2.SP5(下划线)的扩增：
>SP5-F
ATGTACAAAATAAAAGATGTTGAAACGAG_SEQ ID NO：161
>SP5-R
CACCCCTTAATTAAATAAAGTGTATTAGGGTC SEQ ID NO：162
3.PP8片段(加粗)和与SP5基因(下划线)在3’同源重组的引物组
>PP8-SP5-F
Figure BDA0002593461260000261
>PP8-F3R
Figure BDA0002593461260000262
插入序列(MRSA黏附蛋白SR5)示于SEQ ID NO：168。
例8–噬菌体对MRSA的功效
我们针对MRSA感染患者的样本1至6测试了本发明的新PP8(SP5)噬菌体。这些样本1至6是来自临床分离的MRSA阳性患者的样本。该方法的概况如图10所示，结果如图11和12所示。使用PP8(SR5)裂解患者样品1、2、4、5、6。患者样品3仅显示了部分结合曲线，这表明结合的特异性可能不足以提供100％的裂解率。阳性对照是有SA黏附位点的PP8噬菌体。
在对患者样品3进行序列分析之后，通过序列分析和源序列(blast)搜索黏附位点，发现了新的结合位点。
例9-ORF1插入SP6黏附蛋白(46,090和46,091之间)
我们还生成了PP8 SR6突变体，并针对金黄色葡萄球菌对其进行了测试。结果如图13所示。
我们然后产生了新的PP8株以黏附并裂解患者样品3。通过将黏附蛋白SP6添加到原始PP8模板的ORF 1中以生成PP8(SR5,SR6)，使用同源重组产生了PP8(SR5,SR6)突变体。用于该同源重组的引物组是：
1.PP8片段(加粗)和SP6基因(下划线)在5’同源重组的引物组
>PP8-F3F
Figure BDA0002593461260000263
>PP8-SP6-R
Figure BDA0002593461260000264
2.SP6的扩增(下划线)：
>SP6-F
ATGTACAAAATAAAAGATGTTGAAACGAG SEQ ID NO：164
>SP6-R
TCACCCCTTAATTAAGTAAAGTGTATTAGGGTC SEQ ID NO：165
3.PP8片段(蓝色)和与SP6基因(下划线)在3’同源重组的引物组
>PP8-SP6-F
Figure BDA0002593461260000271
>PP8-F3R
Figure BDA0002593461260000272
插入序列(MRSA黏附蛋白SP6)示于SEQ ID NO：169。
所得的新噬菌体株称为PP8(SP5,SP6)。我们将这种新噬菌体与PP8(SP5)结合使用，以确定是否可以使用这两种新的修饰噬菌体裂解患者样品1至6。如图14和15所示，新的突变体噬菌体裂解了所有六种患者样品，证明向本发明的PP8模板中添加了新的黏附基因可以特异性靶向细菌。
例10-ORF2内溶基因插入(在PP8中73195处插入)
1.PP8片段(加粗)和与外源基因(下划线)在5’同源重组的引物组
>PP8-F5F
Figure BDA0002593461260000273
>PP8-内溶素-R
Figure BDA0002593461260000274
>内溶素-F
ATGCGATTCAGTGACAACGGTCTAAGATTTACGGCAGC SEQ ID NO：172
>内溶素-R
TTATGCTGCGTTACGCCCGATTTTCTCGGCAACGTCC SEQ ID NO：173
>PP8-内溶素-F
Figure BDA0002593461260000281
Figure BDA0002593461260000282
使用常规分子生物学技术进行内溶基因的插入。插入序列示于SEQ ID NO：176。
例11–在ORF 1和ORF 2中插入黏附蛋白
ORF1中的同源重组
1.PP8片段(加粗)和与外源基因(下划线)在5’同源重组的引物组
>PP8-F3F
Figure BDA0002593461260000283
>PP8-黏附蛋白-R
Figure BDA0002593461260000284
2.外源基因(下划线)的扩增：
>黏附蛋白-F
ATGTCGCGACTGGCGCAGGATATGAAAAAACTGG SEQ ID NO：178
>黏附蛋白-R
TCAATCAGTATACCCGTATACCTGCTC SEQ ID NO：179
3.PP8片段(加粗)和外源基因(下划线)在3’同源重组的引物组
>PP8-黏附蛋白-F
Figure BDA0002593461260000285
>PP8-F3R
Figure BDA0002593461260000286
ORF2中的同源重组
1.PP8片段(加粗)和外源基因(下划线)在5’同源重组的引物组
>PP8-F5F
Figure BDA0002593461260000291
>PP8-黏附蛋白-R
Figure BDA0002593461260000292
2.外源基因(下划线)的扩增：
>黏附蛋白-F
ATGTCGCGACTGGCGCAGGATATGAAAAAACTGG SEQ ID NO：182
>黏附蛋白-R
TCAATCAGTATACCCGTATACCTGCTC SEQ ID NO：183
3.PP8片段(加粗)和与外源基因(下划线)在3’同源重组的引物组
>PP8-黏附蛋白-F
Figure BDA0002593461260000293
>PP8-F5R
Figure BDA0002593461260000294
例12–靶向大肠杆菌、肠炎沙门氏菌和产气荚膜梭菌物种的噬菌体开发
对目前在加拿大家禽场引起死亡的所有致病性大肠杆菌、肠炎沙门氏菌和产气荚膜梭菌物种进行序列分析，从而可以评估和产生通用的结合域，该结合域用于基因设计可破坏这些致病性细菌的噬菌体。实现该目标的过程如下：
1)序列分析：
从曼尼托巴省(Manitoba)的家禽场收集了粪便和其他排泄物样本，用于鉴定大肠杆菌和肠炎沙门氏菌。产气荚膜梭菌样品由行业伙伴提供。所有这些样品均用于分离加拿大家禽群中存在的致病性细菌以及噬菌体(用于构建我们的噬菌体库)。一旦获得了致病性细菌的纯培养物，就使用Illumine Miseq 2000对细菌进行序列分析。对这些序列进行分析之后，可以获得每种细菌的普遍黏附区域。使用Clone Manager遗传程序，确定了各种细菌物种表面上的保守黏附区域，并对可以黏附到保守细菌结合域的遗传克隆的产生进行了反向工程。
2)将保守的黏附区插入模板并在酵母菌株(CP109)中繁殖：
该普遍黏附构建体被亚克隆到所公开的噬菌体模板中。通过在酵母菌株CP 109中转化和繁殖而产生感染性噬菌体，该酵母菌株CP 109具有保持多份噬菌体模板的能力。这通过两种方法实现：
a)在体内转化并最终诱导酵母细胞中的噬菌体模板，或
b)通过使用酵母细胞的细胞提取物进行体外培养。
无论使用哪种方法，使用这些方法进行繁殖的优势在于避免了经典的噬菌体繁殖，在传统的噬菌体繁殖中，潜在危险水平的细菌内毒素会污染制剂。这些噬菌体生产方法消除了这一障碍，因为酵母细胞被用于培养噬菌体。
3)确定噬菌体靶向和感染多种大肠杆菌、肠炎沙门氏菌和产气荚膜梭菌致病性细菌物种的能力：
对噬菌体进行生长表征以确保普遍黏附蛋白的适当插入。感染大肠杆菌、肠炎沙门氏菌和产气荚膜梭菌，并分析噬菌体的生长。进行了裂解测试，以确保不发生整合。进行了细胞毒性测试以验证酵母中的无毒提取方法。已经分析了噬菌体的结合能力，并准备评估肉鸡中的噬菌体治疗。
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序列表
<110> 噬菌体技术公司
<120> 基因工程噬菌体
<130> 53830-3002
<150> US 62/619461
<151> 2018-01-19
<160> 194
<170> PatentIn version 3.5
<210> 1
<211> 87662
<212> DNA
<213> 人工序列
<220>
<223> 在分离自环境的噬菌体中发现
<400> 1
acaaatagtg aagagataaa ccaggttgag caagaaaaat ctttttattg tagtgcatat 60
agatttatta agagggctag aacacatacg aagatagcct tatcatcaat ttatctaaga 120
attataccaa aatttttagc aaaaagcaag aaaaataaaa gcctgatctg ctgagatcag 180
gcttggttga ttaatcctct ggattaacat ccaggaaatt tttaatagca tcgtgcatag 240
tttctttacg aagctgacgt ccaatcaaaa tatgaatagg ttcgactcga ccattaggta 300
cttgatttgt gttctgtttt gtggtagtcg tgtgcgcccg tttcattaca gttttgccaa 360
aaatattttt aaaatttttt cttgactctt tgaacgtggt tatctagtat ttgcatcaac 420
ggggagggaa tgaccttccc cactaaataa agtcctaaac tggagattca aaaatggcat 480
atcgtgcacc taaattcatc aacaaagaca actttcgcaa cgcactggag aagtcacttg 540
atgaaaattt taaaggcaac attatcgttg ttcacgcatt caattttaag tatgatgtaa 600
acgggaatag aatcaaccat tacacggcaa ccatgttaga tggtacgcta tcaagtgaaa 660
aagctgtact gaatgcactt gctggacgtg gtaaaaacct gataaaatgt gataagagac 720
gttatcaggg tggcgcatat ggttatgatg atgctgttta tcatcttgaa aatatgggct 780
atcaagtaga aaaagctgga gcgcctcaaa taatcggtag tgatggttac gtaactatct 840
tcaaaattaa caaataaagt actttacaag gggcttatag ttcgataaag taagccccag 900
atagagagat ttatcaaata aagcccttaa actaggagat tctaaaatga aattatcaaa 960
tcaagtgcgt gaaaatctga gcaaattact caacaaaatt gatttcattg aaacatcaag 1020
gaatagccat gatgttatgg aatggttaca cggcaaaata aaaggtcgtc gtgtagtgat 1080
ttgcttatgg aacagttgca gggagtataa aaaacctgta ttgcaagtca atatctatga 1140
tgacactttt aaaagtccag taaagtctaa aagcgattta ttagaatcct atgagataac 1200
tgtaagtggc aaagtatcac gtaaagagga aacaccatgt taatgactgg tatcactgca 1260
atcattgcca ttttcgcgct atataaggca tataaagcct ataatctagc aaataaggca 1320
atcacacagc aggttagcaa taaccttgta gagagctttc tagggcgtct cagtgatgag 1380
caaataaagc ggctagatat gagcttcaga tataaagcga aaacctatca gataagtgac 1440
atcttcaaag atgattttca gttagtcgat gattataatt ctctgataaa tgctttaaat 1500
gtcagtgact taaaagacta ctacgctgta attgttcaag agttgagcaa acgaaaacaa 1560
aactgttgac actggttttt agatagatta aattacacat caacggggag ggaatgacct 1620
tccccactaa ataaagtcct aaactggaga ttcaaaaaat gttttgtgca ggtgataaag 1680
ttgtctggaa tgatatagat gacggtttat gtacgaaagt tgttgttatc ttagaaattg 1740
attatatcac tggtgttgca actgtagcag ataatgatat aatctttgat gtcttaatta 1800
gtgaactgtc aaaataaaat ccttaaacta ggagctttta aaatgtatac tactaacaac 1860
ggtcgtactt taaatgtcac actacgtcac tatgtgaacg gtgtaatgca cttcgaggat 1920
ttacaagctg aacagcacat tttggattgg caagttgcag gactccaaaa aactgctaca 1980
ggatacggga aaaagattcc aaccagttgg aaagtatact acgaaggacg tttgcgcaga 2040
atttatcaag atgtgcaaag caatagcgca tcgagttaca ttatagtaaa aggcaaaaaa 2100
ctgcatttaa tgtaaagtac tttacaaggg gcttatagtt cgataaagta agccccagat 2160
aaagagcttt actactcaat cctaaattgg agaacttaaa aatggctatt aataaccgtg 2220
aattatcaat tttaaaagca cgtttgaccg tcaacaggat taatgttctg acatcatcag 2280
cgcctgatga aacattgcac aacattatcg ggaagattca aagtgttatc ttagacgttg 2340
aaaatgtaaa aagttcattg gctgacgttg catcaggtat cacgctagac ggcgcgcaat 2400
atgaaatgtc agacatgtta ggcaaatcta aggtaatgaa taaagagtta gatttgaaaa 2460
tgttccgatt tgctgttaaa gtgtggttat ctgtcgagta tgatgctaac tttgcaatcg 2520
ctgacttttt cgccacttgg ttgcagcgta atttgtcaaa tcacgatttt cgtaatatct 2580
gcgatgcaat ttatgcagaa ctctaaaaaa ttgttgacac ttatttttag atagattaaa 2640
ttacacatca acggggaggt attaaccttc cccactaaat aaagtcctta aactaggaga 2700
ttcaaaatga tgaatttttt aaatatccct gatacttgcc gactgttgac gcaaccagaa 2760
aatttaggca aacctttcct gtaccatatg ccagacaata caccaaaaac atatagctac 2820
acagttaacc gctttacggg ttacgctgca aaaaacggtt acaaggttac tactcaaaaa 2880
gtaacgctat ttacaaatat cgaagagctt ccaacaatcg ctgtaaaact aatatttacg 2940
aagaagacgc aataaagtac tttacaaggg gcttaaaagt tgatatctta agccccagat 3000
aaagaacttt accactaaat cctaaactgg agattctaaa atggctatta ttaacggcgt 3060
atctatcgag actgtacaca tcaaagacat taaagccggt gatacagttc tttttcacgg 3120
cgtagaaaaa actgtcactg caaaggatat taaaggggat tctttcatgg gtaggactct 3180
cttcggtgat tcttattgct taggttatct tgcagtttta aaagttgtca aaaataacaa 3240
ataaagtact ttacaagggg cttaaaagtt gatatcttaa gccccagata aagaacttta 3300
ctactcaatc ctaaactgga gattcaaaaa tgaaatgttt tcacggtact actcaagaaa 3360
atttcctcaa cctgattaat aacggtgata aaccatcagg cgcatggaat tgttcggata 3420
tggacggcaa tttttacgtg tatccagaaa ataaaatcta tggcgacgat gaggaagaaa 3480
tagcatcaga gggcattcag caagctctag gtaatgccac tatcaccgca gcttttcaga 3540
tgaagactca aaatattgtt atcttagaac tcgatattcc agaggatgag ctaaacgatg 3600
attattcttg tgataatatg tcgagtgtag caagttttac agagtacttt gatgtaaatt 3660
ggattaaaaa ggtctataca acagagttta acgctatgta ttcacctttt tgtcttcctt 3720
cattggataa cccaaattta aactatcttg atgagtctct ggagcttctt gctaaaagtg 3780
ttcaacagtc ggacagtatt caggtttttt gtgacatcat ggatacactg acaggaaaca 3840
ttgcagaaga ggatttaaag agctttttct aaaaacctat gcaatcacct tataaacact 3900
aatcagcccc ttgtaggggc ttttaggggg cttaaatcat gcttacaacc gtttacctta 3960
tcctttctat ttgtaatggc cattcatgtg attttaaagg gcttgaggag ttcacaggaa 4020
gtaaagaaaa tgctattcaa gtttgccaga tagcaaggca agactatccc gcaagtgatg 4080
atattcagtg ttactttaag acagaagatg acgacggaat ctatttcgat agtgttgatg 4140
gtcagtatga gattatcatt gaaaaagact agacaagcct gataaaagtc tgtaaattgt 4200
caatcaacgg ggaggaaatg acctccccca ctaaataaag tccttaaact aggagattca 4260
aaaatggctt atgtaaccgt aattaccgat aaagctggcg catcttggtc aactcaagta 4320
agtgataaga tgtcacctat gcagtgccta aaatactttg aacagtggaa taaaggcgag 4380
gacacaagcc cctttcaagt gatgcagatt atccatgtag acaacgaagg gaataaaacg 4440
acactgaata gtgagtacta tgcaagccgt tttgaaacga gaagtaaaac aatgaagctt 4500
ttacgtgaat ccggttatgc ccatatcgcc gcgttaatct gggatgacct gctaaaaagt 4560
cagcgtatca gctatgtaaa acctgaaaaa atattcatca gttaatcaat aacttacaaa 4620
aaacttttaa aaaagtgttg acaatacccc ttgacattgg taactttgtt cgagggggtt 4680
tatctaaaag ggattcactt aaaagactct ttatagataa gctcaaaaaa ttacttgcaa 4740
tcagctatca aaacaagtaa attactaatc aacgggaggg atgacctcac cactaaataa 4800
aatccttaaa ctaggagagt caaaatgaac aagtttaaag ctatcaactt catccgttca 4860
aatgctgtaa tgtctaagcc tgtaaaagat acatacgaat ttcgttgtaa tggggtacac 4920
tttgcaacca tcactaagtc agaaaacggg gcttattatg tacatcgtcg taatgttgca 4980
accgttgtag tctctcactt tatgcaagct gtagcggaat tattaccgtt attcttagag 5040
atttacttag atgagtgtaa aaacgttcac aaccacgtta aaaacctttt aaacggttat 5100
aaaatggctt atgaacgttc tatcaagtcc ttaaacaagt tctataaagc ccctgaaaat 5160
actgtatcaa tagcttatga tgtaagcggt gaaatggtca acctctccaa tgtagaacaa 5220
attggccttt atgacttacg tcctaacggt tacgctaaaa ctctcggtga aggtttaacc 5280
ttcaatatca agaacttaga aaaaacttta aaagatattg aaaaagatat tgacgagaca 5340
atcaaaagtc tgtaaattat caatcaacgg ggagggaatg accttcccca ctaaataaag 5400
tcctaaactg gagttatcaa gatggaaaac gtagaattaa ccacactatt gcagattgca 5460
accgacaaag aagatgtagc attatttcac cgtatcgaaa gcgatttgtt caatggtgaa 5520
atcatcaaag aatcttatga agttgtcgga ttcaaagatg gtaaagcgtt tggattcttt 5580
gacctcgaca aaggcaacgc tgttacaaag tatgatgttt tgtcttgtga gattcttaca 5640
aaatacaacg aaaaaaggct tgcaacatac gccaatatgc agtaaattac taatcaacgg 5700
ggagggaatg accttcccca ctaaataaag tccttaaact acttcctaaa ctggagaatc 5760
taaaaatggc aactatccgc gctactgtta acgagttcgg tttttcctct ctggatgtta 5820
aatttgacat tgcaggcact gataacgcct ttgaaatggt gaaaggtctt atcagtcaga 5880
ccatcaaaga ttataatcct tcggatgagt tctgggagga aacaaaacaa gaagttctca 5940
aggatggtta tacttatcac tattggggag aaaaaattgt ctccctgtac tacaacaaac 6000
aagattataa ctaatcgacg cagcggggct atttagcccc agatacctga acagccccta 6060
tttaaagggg cttttttacg tctatcgaat ccttatacaa tccctttccc tcacctttct 6120
agaatctatc cactgattcg atagaatcta tctcatacct taaaagcaat aactctatag 6180
tctattctat agccttttat caaatcaact taaatacaag cccataaccc gaaagggaag 6240
ggcaacccct aaaacgctct ataaagctct ataacagcct tatcaaccta acttaaggga 6300
ttgcattaac ctatcttaaa aagctcttta cagggcttta tagaggctta aaagaatctt 6360
ataagggtgt aagctttatc atctcgttag ttatcttgtg aactactaaa aagggcttgt 6420
tattgtctta aaagtatgtt ataggggcta taaagttact ttgaagtagc ttaaaagtta 6480
cgtaaatgct agccccctta acaatttctt aacaatccct acatagttat gcttttaaga 6540
cacttgttag caacttgtta gccccttgtt aaatctctgt taagaccacg taaaagcctt 6600
gtaagagtct ttgacatagc cttaaaagct cctgtgaagc cctataaaga gttatctgta 6660
cagttggggt gtaactacta ggaagagact taaaagagct tataaagagc tatagagagc 6720
ttttaagagg tatgtttaag gaatcactaa gaaaggaaag ggaacgataa gaaagagata 6780
gtgaccatgt taatagctag ttaaaaagat taaacacttg ttagaaactt gttaattatc 6840
ttaacaaaat aactctttaa aattaacttg ttagctcttg ttagtcatct gttaagattc 6900
tgtaaaacct atgtatcaac tgttaagaaa ctgttacagc cttgttagtt ctgttaagaa 6960
tctgttaaga cctttgaaga gttgttaaga agatgtgaga actgttaaga gattgttaag 7020
gatattttaa aattttgcaa agtacttgta agggagctgt taagggctac caagcagata 7080
tacatcctaa tcagatattt ttgagttcct tcaaagaccc taaaaaatga cttgacagtc 7140
ttattttata aaaattttaa aattctgaat aattacctac acagtctttt aaaaatacag 7200
gtagaaacag gcttataaga taccttgtta gtaccgtgtt agaccttgtg agataaggtc 7260
tgtaaagata ttcctgttaa gtttgttaaa acagactata tagaggctta gtatttccta 7320
gtgaacacct atagagagtg tcataggtaa catagagatt aagaggtgta taggagcttc 7380
taagaaggtg tagagagatg tttaaggtga ttgtataggt tgttaagaag gcttgttaga 7440
agagtgttta gatggctatt tagaagggct atacagaggg ctattaagac cactagatag 7500
ttactaagat actatatagt actatataac tatatataga gagtaagatt aagaaggttg 7560
ttaagaagga tagatatttt attcacatat gatggtgacc cagaggttgg gacaaccgat 7620
aaaattttat aaaaattaaa aagcccccga ttaaggaggc tcttaagata ttttaaatca 7680
ggctttctaa aactgtcttc aatgatgact tagttgtctg ataaaaatga gtgaatccat 7740
tgttgttatc gaatatctgc atcataacag catctccctt gattgggtac actcggatgt 7800
catagtgaaa tccattaaag acagcagaac cattacagaa tacactaaaa tcatccactg 7860
agatatactc ttcatgacct gtgattttta tacagacacc tttactcggt ctcttagggt 7920
tgatatcttc cataatagac atactctgtc caccaagaga taccatagtg accactttac 7980
ggttcttctg tttaaagtac attttaactt tctgcaaagt gtcttcatag aacggataag 8040
acctttctac aaagattcct tcaagtggct ctttaccatt ttcaacttct ctgttatgcc 8100
gtacttcaaa gataatttgt tcgaaccagt caagattttc ttcggagagt gcttctcgaa 8160
catcactcca gtcaagtact aggtagttac cttcaacttc ttttaagtta tctttttcca 8220
tatctatcac accattatcg taaaacttga tattctggta ctgcgaattt attactattg 8280
ttcttgatga ggtcatcctc tgaaactaaa aatgtaatac cacataagtg tctgtaacga 8340
ctatcatact cgaatatctt aaacagatat ttaccatcat gataaacctg ttgcacttca 8400
ccttctgcaa caatctcgcc tgtaataaaa ttttttagtt tatacacatt accaacttta 8460
ggtactgttt tcatctcagg ctcttttagt gctacaccta tttttgcatc accgagtaaa 8520
tgagaactct caaaagctac gtgtttcata taagcttctg agcattccca gataaaacag 8580
tgtacaagaa ccttagcagc atgtcttgaa cagtttaact tatcacaaat ttctgtgtac 8640
atctcatctt tagtcgagtt gtcaagcatt gctctttgca ctatctcttt tactttcata 8700
agctctccga ataaccctat taagcatctc taaagtcagt tttccagact ctaaatgtgg 8760
cataacaagc tttaaagctt tttcaacatc atgcacaaca tctattatac cttcttcata 8820
ggcttctaca aggatatctt ttacgttttg ttggttatct ttttgtggta ttgctagacg 8880
catattaaac tcatttgctt gagggtggtc actaccacgc cagtaaccat ctttgttaaa 8940
gctgatgtag tcaccgtggt aaaagtgtac accattagct ttaaacttct ccagcacaag 9000
gcattgaatt gggtactcag tattccactc cctgactcct ataaccttca ctgttgcgat 9060
gtactcctgt gtttttgcat ctatgatgtc aagttcttga ccagctatga tttgcatatt 9120
cgctaccttc taagtttcta caaaggggct atcaagtaac ccctcaagtt attactcaga 9180
cttttcagtc ttcttgtcgg atttcttact agtcttcttc tcagctttct gctcaggctc 9240
cttctcagaa gttgccttaa taaccatatc aagaagctct gataagtcac tcttcagtaa 9300
agtaacaggc tgctcattgt agaagttttt gtcaagataa actttaaact cttctataga 9360
aggaaatcca aaaattggta caggtgtctt aaccattact tagtctctcc ataagcatct 9420
ttcaagattg ccttgttaat catttcagcg attgcaaaac gaacacatac agctataatt 9480
gtaggttgca ttctttactt acctcttcaa gtttaacttc agagtcctta agtcgtttct 9540
tatgaactct gaatctcatt agtgagatga tgataccaac taaagggaca gcatacaagt 9600
accaaacatc atataccttt gcagtaatct gtgtaatggt caaatcttga gctatggaac 9660
tgattgctga tactgccagt aatgttgcca agaaaaatgc cagtaccatt actgtactca 9720
aacaacctct ggcatggtgt tcatgcttaa tgcacttatt cttttcgtca acaactctgt 9780
tgtagtcgtt tgtaaagtct ctgtagaatg ctttcaagta ttcttgagtg tagccttcaa 9840
agcaacctgt accataccaa taatcaccac caaaatgtgc tatgataccg gtatctccat 9900
ttggttttgt tactcttagg gagacatgat gaccagtctt ctggtaatct atcttgtatc 9960
tttcttcgta aatcattttc caatccctct caatcgtttg ttccactcaa ggtcaacaag 10020
tctttgtagg actttacctc taggtgttac accttttaaa tctgcgtcat agcaatggta 10080
aaaatcacct tcagcgtgtc tgccatagca tcttcttata ctctgacttc tacgatattg 10140
ttctaataga gacagcttgt agtaatcttc aactgtagtt agttttttca ttctttcacc 10200
atagcatttc tgaactgacg ttgagtttct cttgtaattt cgtctggagt aaaccctgct 10260
ttcagcatat ctctgaaaag aacttctaag tgattctttt caataatgta aattgcattc 10320
tgcacagatg ttttaagttc cttcaggtta ctgctaattt cttcgcactt gttgacaatc 10380
tctgtatcaa ctttcttaaa gactggcatg gtatatctac taccaccatt agcagcatcc 10440
atgaaaaatt taaaagattc ctcagagtcc tttaaagcaa tccctataaa gttcttgtaa 10500
gcacctgtga aagctaaaga ttcaatctca gcagttgagt atacagttcc tgcacacagt 10560
cttgcggagg tactaaatgt gtggtttggt gttatgtcaa agtctaagtt cttcagtggt 10620
cgaaaataaa ctatcccatt gtgtgagatg tgtgttacaa cagcctcaac actctttgaa 10680
ggattctgtt taacatacaa aataatttta tcgtgttctt tcactttagc catatctatt 10740
ctccagaaac taaaaaggga actacctctc gatagctccc atcataatca atattgtata 10800
ctcggtcaag agtatttttt aggcagtaat gcactgcgct tgttactctc ttcactcaac 10860
agtgcgataa attcatcacc tttacccttc cacggactaa atgtagggat gtgttctctg 10920
atagcatcaa tcactgtttt cagggcgtta ttttttaacc accaatcaga cactgctgat 10980
gctacactgc taataagcac catcatctgt gaagattggc tcagagatac atggtgaaat 11040
catagtggtg atgatagagc cattattcat gtaatatgtt cttttttcaa ccttatcaag 11100
aagcttatgg aggtctgcaa agagactcac aacttcttta tggtcaatat ctttttcaag 11160
atttgctctg tatttcagca gtgcagcatc aacctcttta tcattgaatt tgaagtattg 11220
gacttcttca ttcacacata cactgatatt gttggtgttt atcgtcaatt tgcacagttt 11280
gactggagaa ttgatgttag tctgaaaaag tgctacacag ccttctacat ttccgaaatg 11340
ttttactaat gtaacttctg gttggatatg ctcatgttct tctgacatta cagtttcctc 11400
attaaatact ttttaagttg tgtgttaggt ttctcaaaaa cttctataga agatatctta 11460
acagactcac cacgcatacc agtatgtttg aatatgtagt actctagcat ccctatgtac 11520
tttgctggta agtcatgctt gacatatacc gtcacagaat cttcaaactc aacaaccttt 11580
gtaccatctt tgtagttaaa ctctttagga tggttgtagt tgtctatatc gtacattaaa 11640
aagagtcttt tacgttgtaa gtcaagctta tttgttaaag actctttaat atctgagaag 11700
tttttgtaca ctatgacatg gagcttactc atcgttaaac tctctgacga agaaatagtt 11760
gactttgtta ttacctacca taaggtcaaa gcaggtattt tcaaaaggtt tgttgtaggc 11820
atcgtatgaa atatatgggt cagctacttg cttagtgctt aacttcttac ttgatgtatt 11880
taagaggcca ccatacagct tcttgatagc ctcttctaca acagaccaga taatttcgtc 11940
agtaatttct tcgtacaggt caagttcacg ctctagtgag aagtcacaaa tagggaaaac 12000
aacctgtact ttactcatta acgaataacc tgtgagactt gaattgcttc agttactgac 12060
aagtcaccta ctttctcttc tttgataaaa gtgaatgtga gttcaacagg ttcttgctct 12120
tcttgctcaa ctggaatgcc attaagctca tcgcggattg attgagttgt ccagtcttct 12180
ccaccatctg gataatggat ggcgtaaaga gggaactcca tatcatacca gccaaataca 12240
gggcaaccat ctgcatcatc tcgcattctg cgaagtgtgc tacctgagcc tgtaaagcga 12300
agactaatat ttccattaca ctcaaaaatc actgcgtcgg caagtccagc atcttctcga 12360
ccatcatggc caaaagtgtc aacttttact tgaatctctt tattcataaa aacctctctc 12420
tgcttgttct atttttgctc ttaaagcttc gcaatgttgt aaatgatatt tagcttcttg 12480
ttcatgtgtt gctaactgcc tttccagaac agatttatag taacctgcaa gttgctcttt 12540
tgtcaatggg tgctctgaaa taatatcaag tccactttca ccaagagggt tgaattgtcc 12600
atgttcgttg acattgtaag tctcaaactt gaacaggcca ttgttcacag actcaatagg 12660
tgacaactca acaagctcta cagtaaaccg tgtatatacc ttgtcacctc ttgaaaccac 12720
attgttcccg acaatgcgta caagcgagcc attacgagtg tacttaaaag ttccaaagtc 12780
actagcccaa agtttcatag ctttctcctt agaaaataaa aaaggctccc gaaggagcct 12840
atgaagatat tactttggat atacactgtc aagataaatg tcagcttcca tcctacgtct 12900
gtttttcagg ccgtttgaag tgaccttctt acctttgact gtaactttgt tccaccactg 12960
catagcttct gcacaaccta ccttattacc atcgttgtgg cgcttgataa atgttgagtc 13020
ctgcatagct gtgataccga tgttgtatgt ttcacttaca agtgcatcga actcattctg 13080
agaagttgga accttgatag ctttgttcac tgctgcaacg aacttttcaa catccgcgag 13140
aagatactgt tcagcttgtt cagcggtgat tttcattccc atcttaacag gcttaccagc 13200
aatacggata gtcccatatc caattgtagg gattccagca gaatcttcgt aagcctctag 13260
cttaagacct tcaaagaact taatagcttc taaacctttt cttgagagtt gcattatgcc 13320
tcccctgctg ggttaactgt aactgttgct gcattggatg ttacagagcc acctgcacca 13380
gtaactacac aggtgtaact tcctgcatca gcagtggttg caccttcttt ggtgtaagtt 13440
gcagtagttg catcgaggat gtcttcctca cctttcttcc attggtaacc tgtcgcacct 13500
gttgccacaa cattcaacgt caaggtctca ccttcagtga tttcttgatt ggttggctgt 13560
tgagtgatta ctggaagtgc atttacagta actgtggctg cgttagatgt gacagagcca 13620
cctgcacctg ttacaacgca agtgtatgaa ccagcatctg ttggcagagc accagacttg 13680
ctataggtag catttgtcgc tgaaggaatg tcagagccat ctttcttcca ctggtaaccc 13740
gttgcacccg ttgcagtcac ttccaagctg atgtttccac cctcattgat ggtctgacta 13800
gaaggttgct gtgtaatcac aggtagtgcg ttaacagtaa ctgttgccgc atctgaggta 13860
acacttgtcc cagcctctcc agatacaaca caagtgtata aaccagcatc agaaggtgct 13920
actgactgtt ttgtgtaagt tgcagaattg gcactaggga tatcctcacc atccttcttc 13980
cactgatatc cagtggcgtt agttgctaga atgcttagag taagtgtgtc accctcattg 14040
actgtttgac ctgttggttg ctgagtaatt gctggtggct tgatagcatc cttcagctta 14100
gcattcaaca ttgagaacgg attaactctt gccaaccatt cacagtaaat agtgtcaaca 14160
tctttaccat tgatgattgc atactgcaaa tccatgaaga aatcagcagt tctcatctgt 14220
gcaccaacac tgtaaaggag ttcatcacta aaaggaactt tatagtctgg tttatagtca 14280
aacttctcaa cttctgcaat atcagcttca ggccaatacg agctataggt aagtggtaaa 14340
atagcctctt gatatgttct aaaatcatac ctcttaccag cctttacgtt tgcaacgaag 14400
cccttcacaa actctttgaa gtctgggtaa gtctttgctt taatcatttc ttttcagcct 14460
ctaaatgtgc gtttagtcta aaataacctt tgtgattcgg agatacatca ccaacaacaa 14520
tacctttaac cttataacct ttaccaatta catactgaat atccataagg aatcttccaa 14580
gaccaacaat atggaacatt ggatataata gattctcaga gtctggaata ttgatttctg 14640
gtttgtaggt gcgttgttcg cttgttggtg taatatcttc ttcgggccat ttactgagtc 14700
tccatgtcaa aggtgttggg ataacatcat taaggttgta cctttcacca ttttgcacgg 14760
aattgatata ggattgtagg aagcaataaa agtcttttgc actcttagct ttcattaaat 14820
atccttataa atagttaaag gggcatcagc ccctattcac acttaagctt tggtcttaag 14880
taggttctta agttttttaa gattcatact ctcatcaaac ttaaaaccaa atccattgac 14940
ataatcaatg aactcggatt tcttactgag tgacaatgcg taactcatat caaagttctt 15000
acttgtttct gcgtctggac tagagttcac atcaaatgaa atttctgtag gctcttgctt 15060
actttttctc atattatcaa tcaacaaaca tgtatagttt gctggataga aatttacact 15120
accatcaacg tagctgtact tattattatg gataaggttg gaaaatacct taaggaatgt 15180
ttctgagtcg tggcaaacaa gtttgaattg gctgctctct ccaagcgcaa ctaatgagta 15240
cggactttca ctatcaaagt cataatttgg attcgatgac gtcactgtta cacgcatcaa 15300
gttattagca atagtcttct gtttaaaaga attttgaaca tagtgaagac ccctctcaca 15360
ctgattgact agctctttaa gtaggtcatt tgcatatggt gagttaattg atacagagcc 15420
gtcactattg taattaaaac ttactgttaa catttttcag ttctcctgcg atttataatt 15480
ttgctgcaat agagaaacga agtaaactca ctggaagaaa ccactaagtg ttgttcacta 15540
gattgagcca ttgccctgaa atacattcca gtcaaagcat catagtgact attctttcca 15600
attgaggtta tatagccatg actctttgca gtttgatata aatctataat atctgcgatt 15660
tctaattggg aactacacat agataatcct cttgtcttct gtaattttga aagaacaggt 15720
acagaccaat ttcagaggat gaatagtaga ttaactctac cctttttgat tccttacagt 15780
gattggtatt cttacctttc ttcggtttat ttgattttct cattaagtct cctttgtaga 15840
acaaggcaga cctacagctt taagtaagat ggtacagtaa tccatacaac aagtcaagaa 15900
ctattgcaag ttaaaaaagt tgttgacaaa gtacttgaca aggtgtaggc ttataaatac 15960
ttagaagata acttaaaagg ttcctctaaa aaggctattt aaacagatat ctttctggat 16020
atctatcttt tatcttaaaa gccttttaag aggtcttata agaggcatac caatgaaaaa 16080
cagaacaaac aaaggccagt ttaaaaaagg ccagtctgga aacccatctg gaagaccaaa 16140
aggctctcgt aacaaaagtt cactcgtaaa agctcaactg accattgata attctgctga 16200
gtttgctgca aagttgtttg aagcgattgt tacaagggac gctgctaagc ttgcagagtt 16260
tgggttaaac acagacgacg taaccatcaa aagcatgatt gaggtcggta aaactatcat 16320
gacccactca gcaggtgaaa tgaaagcaat tgcagcagac actaagaaga ctcctgataa 16380
tggtggtcag tctcagacag acaacaaacc aacgttctct gctgttgcaa cccttaaaaa 16440
ataattttaa aagatgttga cagactctat aaagttgctc taaagtctgt cacatcaaac 16500
aacaaatgag agaagagagt aaaatatgag cgaattattt aaacatgcgc accttcatgc 16560
aggtcgaact gaaaatggtg ctgtaaacca tacttcatca atgtctgctc ttgtagactt 16620
ttacaaagct gctggttcaa gccgtagcaa tgtagaaatc ttaccagacc tgttctacaa 16680
agctttgcgt gaggatgttg atgttgcagt tcgtattttg cttcatatgc gagatgtacg 16740
agaaggtatg ggtgagcgta aagccttcag aactgttttg cttcaagcaa ttgaagacaa 16800
agttttagag tctacgcagg ttcttcgcat tatggataag attgcagaac ttggtcgttt 16860
tgatgacttc aaaatcttcg taggtactcg tttcgagaca gatgccttca aacatttaga 16920
agcagcatta ctagaccctg caacagcagg tttggcggct aagtggttac cacgagtcaa 16980
accacgtcat aagcagtttg taaaacgttt ctgcaagttt gccaacttga gtgagaaaga 17040
gtaccgcaca ctgttgtctg cactatctga tacagttgag caaaaaatct ctgctaatga 17100
gtttggtaag attgactaca gcaagattcc ttcacttgct gctgcacgtt accaaaaact 17160
gtttaaccgt aaagatggag agcgctacaa agcttacatt gagtccttat caaagggcga 17220
ggctaagatt aatgctggtg ctgtttaccc atacgatgtg attaaatctg ttaagtatgg 17280
taatgcagat gttgctaatg agcagtggaa agcactacca aactggatgg cagaaggtga 17340
aaacatccta tgtatgactg atgtttcaag ctcaatgtct tgggtgaatt ttggttcaat 17400
cactgcactt gatattggtg tatcacttgc cttgtatgta gcagaacgca atacaggttg 17460
ctttaaggat gagttgatgg tttattcaac aaaccctcat ttcattgaac tgagtggtga 17520
tttacgaaac cgtcatcgtc aggtgatgcg acacgttgaa tatggctcaa ccaacttaca 17580
agcagctttt gaccgtattc ttgagacagg taaaagaaac aacttgactc agaaagatat 17640
gccaagtaag cttatcatat tctctgatat ggagttcaat caggttgatg gagctaacgg 17700
tcgtacaaac tttgaagcaa ttcagagcaa gtacaaaaaa gctggatatg agatgccaca 17760
actggtattc tggtacttag caaaccgtaa tggtacttgc gaagtgtccg ttaaggataa 17820
cggtgtagca atggtatctg gtttctctcc agccacttta aaagctctgc ttggtggtga 17880
gaagtttgac ccaatcagcg taatgctcaa agcagtaatg attgaccgtt atatctggta 17940
aaaaagtttt aaaaagggta ttgacaatgt gtttaatacc ctttaatatg ttctacatag 18000
aaacgaaatg agagcttttc taagatactg aaaaatattt taaaaaagtt cttgacaacc 18060
actaaaaaat aatgttaaag tggttacata gagtttgaaa agtttatctc tgtttagctc 18120
agcttggtag agcgttccgt ttggggcggt aaggccggag gttcaagtcc tccaacagag 18180
accaaattaa tgttccagta gacaaaatgg taaagtcacc actctttcaa agtggatatc 18240
tgagggttca aatcccttct ggaacgccag ttttgacaga agaccaatta cagcaaactt 18300
aatcttattc atctgaaggt aaatcggaca aagaagagat ttggtctggt cattaagaat 18360
tgcgggtata gagaaagggc gtctcacatg tctcattagc atggtatcgg caggttcgac 18420
tcctgcaccc gcctccaaat ttacagaaga ccgtttacag caatatttat taaattttta 18480
gcggaaaaat tgaaagatac ggtctggtaa tactaaacat ggttaggaag cacataaggt 18540
atgtgcggtc gcctgttaag cgaatggcac agggttcgaa tccctgacta accgccaaat 18600
ttaggagaga agcgattgct caccgatttg taaggtgcaa gtccttagcc tgaatagaaa 18660
ttaaatgtgt cgttatcccg tagatggtag cggtggggac tgtaaatccc ttgtcattga 18720
gactcggtag gttcgactcc tacacggcac accaataaag gttatgtagt ttaatctggt 18780
taaaatactc ccctgtcacg ggagatgatg agggttcaaa tcccttcgta accgccaatt 18840
ttgagagggc tatttagccc ctcccttaaa gggttcttac gagtatcctt taaaggagcg 18900
gaagaccaaa tacagcaagt acgtatgata ttttcaagcc aatttaattt tgaaaaattt 18960
aaaacttggt ctggctcaac aaatttacag aagaccgttt acagcaaaac ttaaacaatc 19020
tatttctccg gtaaagaaaa ggtgaaggtt cgattccttc actcggcaga ggtcgagttg 19080
gtgtaatggt agcacttaag atgataaaca acggtctggt aattatcttt gtagataagt 19140
tattaagctt acctgtgagt atgcgaccac aaagggtgaa agggtaaatt ctagatttaa 19200
acccaaaaag gcacatcgct acagacctta agcaagtctg ggtagcttat ctacaaagat 19260
aatttaatgg aagtgtagca gaatggtgat gcggcagact tttaatctga caggcgatgg 19320
gttcgaatcc ctccacttct accaatatgg ttcagtcgca gataaggtaa tgcaggggtt 19380
tcataagccc tatgaatatg ggttcgattc ccatctgaac ctccaattgt gggttagcat 19440
aaatggtaat gcaaacggct gataaccgtt agaagagggt tcgataccct cacctacaac 19500
caatttcaat aaagttgttg acattgagat gtgactactt tatagtaacc ttagttttcg 19560
ttgcgtagcg tctattttgc aaatttaaaa taaatgcaaa cgacaatgtt tttctggcag 19620
tagcttgata ggctaaacac cagtgaggtc ttccaatccc tcatcaaaga atttggcgta 19680
ctcgcccacg gtatgattaa taaggtgggc atctttaagg gctttctaag agagtcttta 19740
aagataatta cggggcgtat ctcagcggtc ttctaaaccg ccgttaaccg agtaagtgga 19800
gtatgcgggt tcgagtcctg ccgcctcgac caatttagtt agcagattac ttagacgacc 19860
taagcgggtc ttcctgtagg gaggtggtct gtatctcatg ttttccagaa catgtaaata 19920
atctgagaag gggctttaca gtgttgagat aatacggtaa gcttcgaatt atagtatggg 19980
acgatgttaa gactctaagg catgagcaac ggcctccaaa accgtttcaa aggggttcaa 20040
ctcctccgtc ccatgccaaa gtcttattag gggtaggtag cggctaatgg cagccaaaca 20100
gtcttgaaaa ctgttgccac tgtagagata cggtgagggt tcgactcctt tacttaccgc 20160
ctaataagat ttttaaagcc aagcttcatt cggatgttgc tttggtatcc ctcgtgtatt 20220
gtcgtacaca ctgataaaac acctagtagg tgtcacggag aagagataat atcaagctct 20280
ccaaaggttc tagtcaccgg attaaacaag actatgcaaa gaccttttag gtctttttag 20340
agggcttcta aggttattac ggcttgttgt ggaaccttgt tgcttaggtt cttagaagtt 20400
ctctaaaaag atttaatggg agattgacgg taattggtaa acctaccatc cttagaagtt 20460
ggtgtttgag ggttcgaatc ccttgtctcc taccaaatta atgcaggtgt agcaaaatgg 20520
ttatgcggct gactcttaat cagtaagacg atgggttcaa ttccctccac ctgtaccaga 20580
cattggggtc atagtttata tggttaaaat tcgagttttg caaacttggg aactgagttc 20640
aattctcagt gactccacca attagtgcat ccatagttta aacgggaaaa ttacagtctt 20700
ccaaactgag gttgagggtt cgattccctc tggatgctcc aaattaatgc tgctttcgta 20760
taattggcta ttacacatcc cttgtaagga tggagatgcg ggttcaagtc ctgtgagcag 20820
caccaattca gaggtcaagt gaaagaccgc tttgagtcaa ctgaagaccg taacaaattc 20880
cacggagttg agttagcggc acaacttcag acctctttca cactcgctta gtttatatgg 20940
taaaacatca cccttacaag atgaagaaaa aggttcaagt cctttagtga gtaccatgtt 21000
ccagtatccc aattggtaga ggatgcaagc tcaaaccttg tactagtgac ggttcgaatc 21060
cgtcttggaa caccaatttt gcaggattag ttcaaatgga tagagcaaca gtctacgaag 21120
ctgttaatag gggttcgaat cccttatcct gcgccaattt aaagaggcta tttagatgag 21180
atacttaaag tatgcttcat ggattttctt agctttgtta gagccatttg cagaaatctt 21240
agcagttatc ttagcacctt ttgtagttcc attctacagt gagaagaaag gccacttacc 21300
tttcggattt agatggatgg agacatatga caacccaatt gatggtgacg aaggtcatat 21360
cgaacgatgg gctaagatta gaaagattgg taagctgggt gtctacatgc agagagtcgg 21420
atggctctgg agaaacaaag cttataactt ctcttaccat gtattaggaa gagatgtaaa 21480
agatgttact aagtggaaag gtaatatcaa cgtaagttct gaccctgaag ataatcagac 21540
aggttatctc ctaatgtgga acagtaatgc ttggggatta ttcgctttta tcccatcaat 21600
taaagtcttt ggtaaacagt tttactggag aatttatatt ggatggaaac taaaaagtgt 21660
tgtcccagaa gaaagagcat tctcaagggg aagagttatg ttggcattct ttattcatcc 21720
actaagaaag taaagattta aaggggatta gtttacaagg ttaaaacctc ggtctttgaa 21780
atcgaagaag ttggttcaat tccaacatcc cccgccaatg ctccattact ccaattggca 21840
gagaggccag acttaaaatc tgtgttatgt atcggttcga atccgatatg gagtaccaaa 21900
tttagcggta tagcataact ggcaatgcaa cagtctctga agctgtccta ttaaggttca 21960
aatccttatg ccgctgccac ttctaagggt tcttacgaga gtccttaaat gtggccttat 22020
cataaatggt aatgacccat gctgtgaaca tggtctatac gggttcaaat cccgtaggtc 22080
accccaattt atagtccaag tagcttatat ggttaaagcg cgtgtctgaa aaacatgaga 22140
agagggttca aatcccactg gactaccaat ttcaaaggtg cttaatgaaa gagatgacag 22200
aacaaggtaa ggagattttt aatctcttaa aaaccggtaa agggttttct aacccactta 22260
ttactggtgc agcagttctc ggtggaaccg tagctgcttc tacatcactt gtaagctcta 22320
ttagttctgt aacagaccct acaatgcagg agaaacttgt tgctgctgga cttacaacag 22380
tccttctaaa cagctttaca acaagcctta caagcactac agcaactact aagaccctga 22440
cagactacgg tcaaaaatct attgatgagt tttcatcacg tatgcaggta gcaaaggggt 22500
attccaatgt tatgggtgca gcaggagaac aagttggttg tacaccgttc agtggtatta 22560
tgggtgttgc tacagaatat ggtcaaaaag ctattgatac aattaacagt acactagaga 22620
gtgttaacga tgtattaagt gacttacaag acgctattga tgagggtctt gatactattt 22680
ctgatttagc taatcaggct gttagtaaga ttaatgaagg tatttcaaag attacagctt 22740
atgcagatga agttgtgcag atgattgaag aagaagctgc ccttattgca gagtacctta 22800
aaacgaatat caatgggttc ttagcaggta tcttaccaga ctggtttgac gatgcctgta 22860
aaactggtgt aattgacact attgcaacac cagaaatgaa gaacgcatta cagaaataaa 22920
tggaagatta accctaaaag gtaagggagc agtttgctaa actgccagta gctgagaaat 22980
cggtgtacca gttcaagtct ggtatcttcc tccaatttga atccgtgaca gaaatggcta 23040
tgtgcctgtc tgcaaaacag gtttataagg gttcgagtcc cttcggattc tccaagttat 23100
tttactctct ctcaattaaa ataataataa taatgccctg ctataagtat tcttcctttc 23160
gctaccgaag agtattttta gacagggctt ttttacagag ttatacttta taagaaggct 23220
tatacagtat gtctctgacg tacattgtgg tttctccttg acggtctctg tgccgtcttt 23280
tttaagggga aacatggtta attatttgga gaaaacataa tgagtgagac cgtttacaaa 23340
gaactttatg aagctaataa aaagctagaa tttatgcaga acactatcat ggcaattgca 23400
gatagattat ctgtagcaac aggcattgat attaaagaag catctatgga tgcactcctt 23460
gatgctgttg atgaaaagtt cgaagttaag aaagaagaga ctgctacaga ctctgaataa 23520
ttctatttgc ggaggcgctt aatggactta aatgctgtta agcagaagcg agtggaagat 23580
gttaggaaag tcctagctgg agagttgggg ctttctgatg aagtaaaaga aattattaaa 23640
tcattcggta aagacccctc taaattcctt ccaactcaaa ttctgacttt attaagatac 23700
acaccagacc aagttcgcct catctttaaa ttgatgactg ataagaacta tgtagcccct 23760
caaccgggtt ctcaagaggt ctttttaaat actaatgctg acttggttct ctatggtggt 23820
gctgctggtg ctggtaagac tgccgcattg ttaatggact ctttaagatt tattgaagac 23880
cctaactata atgctgtata tttccgtcga aatacaacac agttacaagg tggtttatgg 23940
cctgctgcaa agaaactatt tggtaagttt ggtgggattc ctcatgagca gaaaatgact 24000
atcacattcc cttctggggc aactatcaag tttacctacc tagaacttga aaagcacgct 24060
gaaggtcatc agggtattga atactcagca atctactttg acgaaggtac acacttctct 24120
gcttcacaga tttcatacct acagactcgt ctgcgttctg gtgctgaagg tgattcatac 24180
atgaagattt ccatgaaccc agacagagac cactttattt atgattgggt agaaccattc 24240
ttagatgaag aaggttatcc agaccctgaa aagtgtggtc gtattcgttg gtatgtaatg 24300
aatgatggtg tgatggtttc tgattgggag agagacaaga ttcttgaaat gttccctctt 24360
gagattcctc aaacatacac cttcatctct ggtacgattg atgataaccc aattcttgac 24420
ttcttagaac ctaagtaccg tggtaagttg gaaaacaaca cccctgtaaa cgttgcaaga 24480
cttcgtttcg gtaactggaa ggctcgtgca gaaggttcaa actactggca aagacaatgg 24540
tgtgagattg ttgattcact cccagaagat gtatttgatg ttagagcatg ggacttagca 24600
gcaactttac catctgagat taaccctaat ccagactgga cagcaggtgt taagatgggt 24660
aaatctaaaa aagacggttg ctattatatc attgatgtag taagatttag agatagaccc 24720
tctggagtcg aaacacaaat taatttgact gctgaaagtg acggtaagcg aactggtatt 24780
tttatccctc aagacccagg cgctgctggt aaatcctacg caacatccct catcaggaaa 24840
cttgccgaga aaggctatcg tgcaagagct aaaccaacaa ataaagataa agttacccgc 24900
tttgcgggat tttctgctgc ttctgaagct ggacttgtaa aagtcttgag aggtagttgg 24960
aacgaagctt actttcaaga gcttgaaggc ttttgtggtg atggcaaaac taaagatgac 25020
caagtggatg ctaccagtga tgctttcaac agtcttaacg aagttaaatt attcaagcca 25080
ccatcaatgg gtgctcacac agacttagtg agaggaaacc catatgaggg gcttagacgt 25140
tgatagctag gtgagaagaa tggcagatat tacagaaaca caagaaagct taccaccatt 25200
tagaatgggt gaagtaggtt ctttgggtct gaaggttaag aatggaagaa tctatgaaga 25260
acctcgtcag gcactaaggt tccctgaaag tattaaaact ttccaattaa tgatgcgtga 25320
ccctgctgta gcagcatctg taaatattat taagatgttt gtcagaaaag tcaactggag 25380
attcgtacct ccaaagggaa aagagcaaga ccctaaaatg cttgaaagag cagacttctt 25440
taattcttta atggatgaca tggagcatga ttgggcagat tttattaatt ctgtaatgtc 25500
attctgcact tatgggtttt gtgttaacga gaaagtttat aagaagcgac agggtaaaaa 25560
aggaaagtac cagtcaaaat ttgatgatgg tctaattgga tgggctaaat taccaatcag 25620
aaaccaatca acacttgata agtggtattt tgacgaagac tttagaaaag ttactggtgt 25680
tagacagaat ctgagaaatg tttcacatat tgctggagca attaatcttg gagaaagacc 25740
actaacaaga aaacttccac gagctaaatt catgctgttt aagtatgatg atgaatatgg 25800
aaacccagaa ggtcgttcac cattacttaa tgcctatgta ccgtggaagt ataaagttca 25860
gattgaagag tacgaagctg ttggtgtttc aagagacttg gtaggtatgc caaagattgg 25920
tttaccacca gattatctgg atgaaaatgc agaacctgaa aagaaagctt tcgtacaata 25980
ctgcaaaact gttgttaatg atatgattgc taatgacaga gcaggtttaa tctggcctag 26040
atatatcgac ccagatacta aagaggatat ttttgagttc tcattagtct ctagacaggg 26100
tgctaaagca tatgatacag gttctatcat tgacagatat tccaagcaaa ttatgatggc 26160
atttatgtca gatgttcttg ctatgggtca gtcaaaatat ggttcattct ctcttgcaga 26220
ttctaagaca agcttattag caatgtcagt agatattttg ctgaagcaaa ttaagaacgt 26280
aattaaccgt gatttagttg cacagactta tgctcttaat atgtgggatg atgaagaaca 26340
tgtacaaatc acatatgatg atatcgaaac tccagacctt gaagcaattg gttcttatat 26400
tcagaagact gttgcagtag gtgctttgga agttgacaaa gagctatcta acaaacttag 26460
agagcatatt ggtcttcctc ctgctgatga gtctcaacca gtatctgaaa agctttctcc 26520
aaatagccaa agccgctcag gagatggtta taagaccgct ggagaaggta ctgcaaagac 26580
accttcagcg aaagacccaa gcacagcaaa taaggcaaat aaataatggc tgaagttatc 26640
tctatttcaa atgctacacg agtgcattcg tataggggtg tccttatcat tactgataag 26700
ttatctgtag aggctggctc aagggtcagc ctttcaggtt atgttagtga tggtggaatc 26760
tctgacgttt tcactatttg caggttactt gatgcaccaa tgagtggaaa accgtttatt 26820
tcaggaaatt gtagtgaaat tgttaaaatt ccatttgaca gttcatgcct tttgggtgta 26880
aagttatata actgcgagaa taaacgtatc aatgttaata gcattgaagc cgctttcatt 26940
accctcgata ctgcatttca atctccaatg acagttaata aagaaacaaa cagacttgaa 27000
tacatttttt cacaaaatga ttacaaagta cttgtcaaag gtaaagtata tgatatgatt 27060
gtaaatgtgg tagatgaatc tggtaaccat tcaacagtcc ttaaacaaaa agtaaggttt 27120
aattaatggg aacattaact attgatggta agaataaaat cctcgccacg ctaaccccaa 27180
cgactattat tttacacaat gtagacccaa cggcagaccc tacagcaaat aaggttactc 27240
agccagtggc tatttatttt tctgaaccgg ataatggctt aattgcctca gaagacacag 27300
ttaatattac tgtcccagct tctgcaacgg tctcacacta tagcttgtgg gatgctaacg 27360
ataaatgcgt ggcaactggt gctcttagca aacctcaatt ctttgctgaa gaaggtatct 27420
atgttatctc ttcagtctct gtagatttaa acaaataggg tgagtaaaaa tgagttcgaa 27480
tatcttcaga cttgctgata gattattcaa ccaaccttta ctagccactg aatcattagc 27540
tcactcagca gcaacttatg tgaataacag attgctgggt gatgtccaag cagcagtaaa 27600
ctttgataaa cccaaaggtg aagcaagaag tcttttaaaa gtaaaagatg acattgctat 27660
tatccctatt atgggtggtt taacccatcg tatgacattc attgatgcaa tgtgtacagg 27720
tggattaagc tcttatgaag gtttgcgtag aggctttgac gaagctttag cagatgagtc 27780
aatcaagact attgttctgc acattgattc tggtggtggt gaagcttcag gttgctttga 27840
attagcacgt cacattatgg cttcaagagg ccaaaagaaa attattgctt atgtagatga 27900
gttcgcttgt tccgctgcat acgctcttgc atcttctgct gaagaaatta ttgcatcacc 27960
agatgcagat gttggttcta ttggtgtaat tatggttcat caggaattaa ctaaggcatt 28020
tgaaaagaat ggcgtaacaa ttaacgtcat caaagctggt gagtttaaag gtatgggttc 28080
accattccaa gcactctcag aagaaagcaa agaaagactt caaaagagaa ttaatgatac 28140
ctacgcaacc tttacaggtt ttgtagccga atctcgtaat ctctctgaag aagctgtaaa 28200
gaatactgag gcgaatgttt attctgctca ggaagctctt gaacttggtt taattaactc 28260
aatcatgtct caagatgatt tcttaaatta cttacaaggt tctgaagagg ctcctgtaag 28320
tttaaacgtt aacaattcag gtgaagaaat gactgaacaa gaaaagcaag aactagaagc 28380
tttgcgtctt caggttgctc aaatgaaagc taaagaacag gaagctgctt tgtcagattt 28440
gactaataag atttctgctt ctgctgaagc ttttggattt gatgcaaaag aagctgcaac 28500
gactatttta ggtgctggtc ttgataaccc tctgagtgtt ctgtttatga atgctatgga 28560
aggtgctaac cagaaactta atgaaactat cgcgtcccat gcttctgcaa tggaagaaaa 28620
agaatcagaa attaccaagc tgaaagaaac tgctggtgct gttcttgaac attccaacgc 28680
tatggaagaa atgggtaatg acggcgaagc tgatttggtt gaagaagaaa aagaaccagc 28740
taagaatgct tccgaagaca ccgctgaaca acgcaaactg gctctccaga atgctctaaa 28800
atctcttatc aaataaggaa cacaataatg gcatatcaag attttactaa gttaggtaaa 28860
agagaacctc tgaatgatat cattctttgg gaacaggtta ccccaacagg ccactctcgt 28920
aaagagtaca ctccacaggc atctacagaa tatcgtgtag gtgaagttct gaaggaagac 28980
ggtactaaag tcgcagcagg ggaagaagct caggctgatt ctgtatgtat cgttaacttt 29040
tattcagacc tgcaactgtc ttaccacggt cagttgaaag ttgttggtat ttaccgtgac 29100
gcagaactga aagacatgct tactcttgag tctggtgtta atgctgagaa cgtcaagaaa 29160
gctctggctg ctaaaggtat tgatttcgta ccaactggcc tgtaataaca ataataagac 29220
attctggaga attttacaat gttgactaat tctgaaaaaa gcagattttt ccttgctgac 29280
ctgactggtg aagtccagtc tatcccaaat acttatgggt atatttccaa cttaggtctg 29340
ttccgttcag caccaatcac ccaaactact ttccttatgg acttgactga ttgggatgtt 29400
agcttgcttg atgcggtaga ccgtgatagc cgtaaagcag agactagcgc acctgagcgt 29460
gttcgtcaaa tcagcttccc aatgatgtat ttcaaagaag ttgaaagcat tactcctgat 29520
gagattcagg gtgtacgtca gccaggcact gcaaacgaac tgactactga agctgtagta 29580
cgtgctaaga agctgatgaa gattcgtacc aagttcgata ttactcgtga gttcctgttt 29640
atgcaagctc tgaagggtaa agttgttgat gctcgtggta ctctgtatgc tgacctgtac 29700
aagcagttcg acgttgagaa gaaaactgtt tacttcgacc ttggcaaccc taatgctgac 29760
atcgacgctt ctattgaaga actgcgtatg cacatggaag acgaagctaa gactggcact 29820
gtaatcaacg gtgaagaaat tcacgtagtt gttgaccgtg tattcttcag caaactggtt 29880
aagcatccta agattcgtga cgcttatctt gcacagcaga ctccgctggc ttggcaacag 29940
attactggtt ctctgagaac tggtggtact gacggcgttc aggctcacat gaacaccttc 30000
tactacggtg gtgttaagtt tgtccagtac aacggtaagt tcaaagacaa gcgtggtaag 30060
gttcatactc tggtgagcat tgatggtgct ggtgcagagg ttggtgtagg acacgctttc 30120
cctaacgttt ctatgcttgg tgaagcaaac aacatcttcg aagtggctta tggtccatgt 30180
cctaagatgg gttacgcaaa tacacttggt caggaactgt acgtattcga atatgaaaaa 30240
gaccgtgacg aaggtattga cttcgaagct cactcttaca tgctgccata ctgtactcgt 30300
cctcagttgc tggtagatgt tcgtgctgac gctaaagact aatattctta aggagggtta 30360
tgaatgtgtt atacaggcga cccagccaat aaccctcttg atagagtaag aatcctctgc 30420
acagacacca ataatgatga aattcttatt gaacagtctg tgctagagtg gttctatcta 30480
gaatctggaa aggatgaaaa gaaagcagcc atcaaagctc ttaaatattt actctttcaa 30540
gtagccaaga tgggagatga gaaggttggt ggtgtttatt tacgtaactc ttccagattc 30600
aaatctctga aagctgttta tgacgacctt gttaaaagct ctgtttcagg actaccctat 30660
gcaggtggta ttaatcagtg tgacattgat atgcgtcgtc agaatccttg ctctgtcaag 30720
aaatacacag aatatggtga tgctgccaga tacgaaggca gagattactg caaccgtgtt 30780
aatggcgtat ttattatcga gcgagatgaa taatggttaa aagggttatt caccccgcta 30840
gagcaaaatt agtcggggct atgaagaact tgcaaacggc taatgctcaa gttgggtatt 30900
ttcaagaaca aggtcaacat agctctggtt tttcttatcc tgctttaatg tatttacaag 30960
aagttattgg ggttccttca gcttctggta aagtatatcg taggttgttt gaaatcacta 31020
tgatgctaaa cagacagacc ttgttagagc agactaagaa gaatctatat aagcaactta 31080
gcagtctcaa cgtagacccc tcagatacct tagaagcatt tgcaaagaat gctcaaaagg 31140
caattaaaag aggttttggt aattctgcta tcctccctcc taacgcacct tccacagtca 31200
agaaaaaagg ctttaatgca cctcttgttg agacaggtga cttaagggat aaccttgctt 31260
ataaaatttc tactaagaag ggtattaaga aatgagactc ttaaacagac acagctttgt 31320
agtaaagcgt aaagtctctg aagacggtta ttataatgat gatggtgatt gggtagcttc 31380
acaagatatt gtagaggtta actgtaaagg taatatccag ccatatatca aaggttctgt 31440
aaagaatggt acacagattg ctttaccgga aggtattaga ctaaccgata caagaatcct 31500
gtatactaca tataaactta gaacttcaga tgatgtagag tggaatgagt ctgatattgt 31560
tatgattgat ggtcatgagt atgaagtatt tatgactatg gattggtcac agcaattagc 31620
ccatacatcc cattatgaat atatcattat tagaagggat aaaatgaatg cagttagaaa 31680
cagcagaact tgaaaaaggt ctagttagaa ccttagtgga tgttattggt cacagattag 31740
ctcgtgataa aaataataga ccaaacgtaa ttagagctta cccttctgat aactcaaatg 31800
acaaaggttt aaaacctgac cagccattta ttaccgtata ttgtcaagat gctgcaacac 31860
cttatggttg ggttcttgat aagtttgttg aagatgatgt agtttgttac agaattgctt 31920
ttcagattcc tgtattaatt acggtaaatg ggaaaggtgc tcacagtatt atgcttgagc 31980
ttaaacaacg attagagatg agttcagtca gggatttaat tcttgaagag acaggagcta 32040
cagtgctaga cactggagcc atcccaaatg actacactta tctcaataca gatttcgaaa 32100
attctgcgcc tcttgttgta actcttgtaa aaaattcagt cctgaaagat gaacgcggaa 32160
gtattattga gcgtgtcatt gttgatggcg agttggttta tgaagaagga caagagccac 32220
cagaatatac tatccatcta gatgtagact ccaaaggggt aaaataaatg tggaatccaa 32280
ttgttaatgt agatattaca ttgaacaccg caggaactac aagagaaggt tttggtttgc 32340
cactattctt agcttcaaca gataactttg aagaaagagt gcgtggttat acttccttaa 32400
ctgaagttgc tgaagatttc gatgagaata ctgctgcata taaggctgct aaacaacttt 32460
ggagtcagac tcctaaagta actcagcttt atattggtag atgcgctatg cagtacactg 32520
tatcaattcc tgatgaggtt actgagagca cggactactc aattactgta gctgtcggtg 32580
ggggaatgtc acaaccattc cagtacacct cacaaggtga agatactgcc gagaatgtat 32640
tacaacagtt taaaacacag attgaagctg accaatcaat taaagataaa gtttcagtca 32700
atgttactgg tagcaacggt tctgccacaa tggtcatctc caaagctggt gacaatgatt 32760
tcgtgaaggt aaccactaca gcacaaactg tgtctattgc aagcacaact gctgatacag 32820
cgtcaactgc tctggcagct attgaagctt attctaccga ctggtatttc attgcagcag 32880
aagacagaac tcaacagttt gttttggcaa tggcttctga gattcaggct cgtaagaaaa 32940
tcttcttcac agctaactct gatgtagcag cactgcaagg tacagaatta aatagtgcaa 33000
atgatgttcc agcacagctt gctaagagta tgtatactcg cacagtttgc ttgtggcatc 33060
acacagcggc agaagattat ccagagatgg catatattgc ttatggcgct ccatacgatg 33120
cagggtcaat tgcttggggt aatgctcagt tgactggtgt agctgcttct ttacagccag 33180
ctaataagag acctctgaca agcattcaga agtcagcttt agatgtacgt cactgtaact 33240
ttatcgacct tgatggtggt gttccagtgg ttcgtagagg gattacttct ggtggggaat 33300
ggattgatat cgtccgtggt gttgactggt tagaatcaga cctgaaaact tctctgagag 33360
acttgctaat taaccagaag ggtggtaaga ttacttatga tgatactggt attacccgta 33420
ttcgtcaagt cattgaaacc tctctacaaa gagcagtcaa cagaaacttc ctgtcatctt 33480
acacagttaa tgttcctaaa gcctctcaag ttgctttggc agacaagaaa gctcgtatcc 33540
tgaaagatgt taccttcgca ggtatcttag caggggctat cttggatgtt gacttgaaag 33600
gtacagtggc ttacgaataa tagaggtaaa ttggaatggc tatgtatcag caatattccc 33660
ctaaagacgt tgtatgtagc tggaatggta ttgccattga aggctttgcc ccagactcat 33720
ttttacgtct acagagaaca tcaccactta tcacaccagt tgtaggtgca ggtggtcagg 33780
ttgctctgac aagaaatgca gacaagacag gtactattga gattgagcta atgcagactt 33840
ctctctctaa ccagatgctt tctgcaattc aagctaaaca agacaacatg gaacttgaag 33900
aagatatctc ttctaacttc gtaatctacg acccatcagg ctctgttctg gcaactggta 33960
ttaatgcttg gttgcaggaa ttaccacaga ttgaacttgg gcgtgaccag aactctaaaa 34020
cttggatttt tggttgtgag aagctagact acacttctac aattccagcg tcaagtgttt 34080
aataaatcct ataaggggga gactttaaaa ggtcttcccc ttttttgttt cttttaaaag 34140
tattaaggaa tcacaatgaa aacagaatct agagtaatta atggtaagaa agtaaatatt 34200
gttctgcttg gggcaagaga tgggattaag atgtctatga agctgggtaa aattgttgtt 34260
ccaacatttg cacagatgct atcaagtctg actgataaag ataagaaaga agctccaatg 34320
gttccattta aagaccttgt tgaagcttgt tttgacagaa ttgaagaaat taaccttgaa 34380
gaaatggcta ccctgttatt tcaaggggca actgttgatg acttcccact caatattgat 34440
acatacttcc aagcaaacta cggtgagttt attgattact tagcatttgc gctggaggca 34500
aacttcggaa gttttttcga agcaagcatt ttcaaaagcc taacttctca gtaaacatgg 34560
gtaatactct acagacacca ctaactgatg ctgctgtaga ggcaacctat gaagaagcag 34620
acgagatgaa atttgtgctt gctatttatg gtatggaagg gtgtaaagaa acacttgacc 34680
aactctttgc tatgacattc tctgatttat tatcattgag acaatttctt gaaattcaga 34740
ggtcgtataa agaggaaatt gcttacaacg aacttagaag agcaggaaaa atgtaatggc 34800
acaatataca gttgatagct tcattgtgga acttggtttc agtgaaaagg ttgttaaggg 34860
cttgcaaaga gttgagaaaa tgtctatgca agctgctcag cgtattgagc gaaatatcaa 34920
taaagccttt gatgtgaaac ctaataaaag ttctcaggaa gcacttaata gaattgtaaa 34980
aaatgctcag tctgcttcgg gcagaatcaa taaagcactc aacagttcct tgaaccttga 35040
ttcacaaggt gttaaatctc ttaagaaact tgaaactcag gcaaaaaaga ctgcaaaagg 35100
tattaacaag tcattaagag atgctatgaa ggttgacggt aaaatcacta tcaagacagg 35160
gagagggaga ggtggacaga gtatccctcc tgttggtggt ggagcaccca gaggtccgag 35220
agtagatgtt gctcagagac aaatggaaag aatgtttaac aacaacttct attcagggtt 35280
aacccgtaga ctggaaacaa tcggtggtca aggtaaccaa atggcagcct ctttcagagg 35340
aagcttgcaa aatatctata acagatataa aggtactggt aaggttggtg agtatgagat 35400
ggaagttaaa aagctcatcg acgtaaccaa acgttgggtt attgcagaaa atgctagact 35460
aaaatcagtt aaagaatcag cttggctaca ggatagagct aacgcatcat tacgtcaatt 35520
agttggtgga tttgtttcag cttatgcttt actggaactc tcacaaaaaa ctattgaagc 35580
tggtgtaaaa agacaatctg cacaattagc ctctacagct atctttggag cagacactca 35640
gcaagctaga atgttcgctg catcattcgc acaccaaatt ggtcagaact acacagatac 35700
catgaagcag tactcaaact ttgctgctgg tgctcaacca acacttggct ttcagggtac 35760
tcaagagttc tataagaatg ctgcaatgtt tgcccgtatc aggggtgcta ctgatgaaga 35820
cttgaaaggt atcatggttg cattccagca gatggcgtca aaaggtaaag ttcaggctga 35880
agaacttcgt ggacagttag gtgaccgttt agcaggtgct gtgcagctat tcgctgatgc 35940
tattggtaag accccacaag aacttgataa gatgatgcaa gacggtaaac ttcttgctca 36000
agacgttctt cctaaagtat ctgaaagaat ggctgaactt gtcaagcaag caggtggtat 36060
gaatgctgtg tccaagcaga ctgctacatc aatgggtcaa gcgaaggcta tgtgggataa 36120
cacacttgta gcactgttta acaattctag tgagggcatt tcacagttat ctaactccgt 36180
tgcaatgttc ttgcaaggtt ctatgggtag cacgcaggct ttaggtcttg tgattgggaa 36240
cctgttaaaa ggcgctagta atctacttga cttcattaca gacttcatgt acagaacatc 36300
tgcactatac tactatgcaa gagcttggta taaagacctt gacaacagcc agcaaaagct 36360
tatcaaaagt gctggtgaat ttctaggaac agttgtcaca attggaggcg cagttgctgt 36420
agtatcaaaa gcagtcaagc tcctaagtgg tttggttggt ggtggtatct ttggtaaaat 36480
cttacaaaga cttggtgtta gtgcagcagg tacagcagca gccggagaag cagccgcagc 36540
agcaggtgga gttacagcaa cgagaatggc acttggtact gttggttcag cattaatgct 36600
aagaggctct acagacccaa atgctgctaa aaactacagt gaagttacat taccaaaacc 36660
atttgaaaat gctgttgcaa atattacaaa cccaaaaagg ccaatgttct ttgatgagaa 36720
tggtcaactt cagtttgcac agtacactca agacgttgaa ggcaacagaa agttaattga 36780
taatggtcta tctaattggg agattatcat ggagaagcta tcaacatcta ttgataattt 36840
tgccaataag tttaaccaga caccaatgat gatgacacct tctggtttac ctatgcagac 36900
taaacaaacc ctgaatgtta ctttcaatct tgatggtaaa caaattgcta ctaagatggt 36960
ggatattact gacaagaatc aagaagacat tcttctaagt tcaagctatc cagaggaaga 37020
ataatgttat gggattctaa tatgcaaatc aaatatagtg gcaaagacgg catctatttc 37080
cacttaagag ataatgtaga tgccttctta accttatcag caactgaaaa tatggaattt 37140
gacagcccta tgcaggtaac tacacagaac atgcaatcag ggcaaaccgt cacagataat 37200
gtgcaaagag cacccagaac aatcactatt agtggtgtcg ttgtagttgg ctatgaagga 37260
agcttattat taactcgtca gggtcaatta gtagaaaact tcatcgacac tcttgaaagc 37320
tggcgtgacc agaagcaaat tatttcagtc atctgtaaag acggaattaa aattgatgat 37380
tccattatca caagttttaa agcctctaaa gatgttggta tttcaaatgg tctaagaatc 37440
cagctaactt ttcaggaaat taactttaaa gctatcgtgg gacaaactga tatttctgct 37500
gcaactggaa agaccgctac cacgaatgat ggtggtgcta ccagtaaaaa gaatacaggg 37560
aataccacaa caagtctagg taatgggaaa ttaaactgtc agttattatt cgaccttgat 37620
gccaatggag taagggaact tactagtgct gaagataatg cccttgcaaa atgttccatg 37680
tctgcaaaaa ccagaaaagg tgttactaca ttcagtgaag aggctgagaa aaatgccgga 37740
gcagcattaa acagaactgc tggagatgga aaagcattac aaaagcattc agtgaatccg 37800
aataaaaagg ggacttatta atgtcacaat atattcctgt tcctgataca ggatggtcta 37860
cacaaactgt aactctggat ggcactgtct ttgtaattga attaaagtat aaagagaggc 37920
ttgacagatg gttcttgacg ctatctgatg ttgatggtaa tgtattatta tctgaaaaga 37980
aatgccttgc agaccaatca atcacaggac gctatgtaat tccttcatta gctggagagc 38040
tttttgttga acgaatgtat ggtactgatt tacagcctac cagaaataac ttcggaagag 38100
aaaaggcatt tgagcttaat tattacactc aagaagatat gagattaatg gagaacttgt 38160
aatgtctgta aaagatagca ctgctggggc ttcttttagg tgctatcaat tggctgtagg 38220
tagtgaaact acagccttta atgataaacc tacaagccat gctaaagatt ctatgcaaat 38280
ggactatttt gacaacttac aatttacttg taatgtgtct tatacgtctc agaagaataa 38340
agtaacctct gatgatacca cttttgaaat ctacaacctt aataaagaga tgagagccaa 38400
attcaaaacc gttggtgcaa cagttatgct tagagcaggt tacactactg gttttaaaag 38460
ggacgcaaat ggcgacctta ttattgaata tgataacctc ccattaatct atctaggcac 38520
tattgaatat gcttatacat ataagcgtgg tgttgatatg attacaaagg ttatctgttc 38580
caatgataaa atggaaagaa ccacgattaa gacatcaatt tcttataaag cagggacaac 38640
gcgtaaaagt gtgattagag atttagtcaa cagactaggt ttctcactta ttgatgaaga 38700
cctttcaagt attgatagtt acacttacaa gaatggtttt agtgtttggg gaagtgttgc 38760
agaggcactt acaaaggttt gtgaagaaag tagtctacgt tggtatacat ttaacaagca 38820
aatccgagta gttcctttta acgctaaggc tagacaactt tcttgggaaa tttacccata 38880
taacgttatt gattctttgc aaggctacta cagaagaact agaaaggttc tgaaaaaaga 38940
gaataagaca gttattaaag ttaaaactgg agttcgttgc aaaatccatt tagatgggag 39000
aattaagatg ggtgataaca tcactattag ggaaagtgaa gattttgaag gtcagtatcg 39060
agtaaaaggt ctttctcaca atcttgactt tactggtggt gcttggacaa ctgaactaga 39120
tttagagaag gtggaataat gaagtcacca gttactagaa tgtctgggta tgtttcagaa 39180
tgtcttgatg aatttagaaa agaactgtat actggattac cagccattat tcagtctttc 39240
gattcaaaga ctcaaacagc cactgttaag ccactttact ctattaacgg tttacctatg 39300
ccagagatta ctggtgtacc tgttcaattt ccaagtggtg gtggggcatc tttaacattc 39360
cccgtaaaaa ctaatgacag atgctggtta gctttctcaa tgttaccttt agatgacttc 39420
gttgtcaatg acaagaatgt tcagatggaa acaaacatga gaaggacaca tgatatttca 39480
gactgtgtag ctttcgtagg catctgcaca agaacacaga attttaaacc agacccaaca 39540
gcagttagac ttcatttcgg tgactctgtg ttaagagtta ctgatgatgg taacttctac 39600
tttgaaggtg atgtacacat ttctaaaaac ttgtacgtaa cagaagaagt acacggctca 39660
gattttatca gtgacacgac tggtgtgagc tttaatgaac acacgcacca ttattactgg 39720
acagaccctg ctggtgaggc tgatactaca gaggcacaat aatgaaaaca gactttgcat 39780
taaatctagg tggtgactat gttgccactt taggttcaga ttcagtgtat gtggctcatg 39840
gagatttaaa gattactggt aaccaaatta gaattatccc agaagatgat aaagctactc 39900
aggttgctca aagacttcat attagatgcc ttttaagggc tggtgaagtt ttctttaata 39960
catctgctgg gttcccatat ttacaacttg ccaaatttaa acagaaaact tctatctttg 40020
ataattacat gaaagcctac cttgttgaaa caagagatgt atctaacatc tataactatt 40080
cttcttcaat ggataatgct cagagaaaag taactgttaa ttttgatgca actactacaa 40140
cagatatttt aacagacatt acgcaagagg ttaatatcta atggcaggat taactacaac 40200
aggattacaa actctaagat atcaggaaat ttttgataat atcaaatcaa ggcttcttag 40260
agatatttca ccaaaccttg acgtttctga ggatagtcag ttaggtctct ttctagcttc 40320
aattgcaagg tctttagcag acactcatga aattctatca gaaatctatg atggtggaac 40380
gattgataaa gctgaaggtt ttaaccttga tgatattaca gctttaaacg ctgtgtatag 40440
atatgttgct caagctacaa ggggtcaagt agagtttact ggaacaacag gtgcaacaat 40500
tccgtctaca accagattaa gaagtactgc tgggaatatc ttctatccag tttcaaacat 40560
tacattaacc ccttcatact gtgttgaggc tattcttgaa gttaactctt tacgaactga 40620
tgcaaattat gttattatta ttgataacgt tattttctct tatcagccaa aatcttcaga 40680
cacaatcaca gtactattaa ctgagcttgc taatgcaatt aatggtggta ttgtagcaaa 40740
agctgaagtc atcaatgatg gttcagcatt acgagtttat aaagatgaag gcgacattat 40800
tgcaagaacc aaccctatgg tagtgactgc taccacattc ctcacattca caaaaattac 40860
aactatccat gatgttgttg ctgaggaagt tggtgcaatt cctgcattag ctgggacact 40920
tatcgaaatt gaaacaactg tagatggtct tgatagcgta tataaccgtt atggcctgac 40980
aacaggtaga aatgaagaga ccgatacaga acttcgtcag agatatttag aatccctgtc 41040
agttactggt gtaggtactc tggatgcaat cgtagctgct gttaagaggg ttcagggcgt 41100
atcagatgct tcaggtgttg aaaatgatac tgaagagaca agctcagaag gactcccacc 41160
aaaatctttc aagattgttg tagttggtgg tcaaaatgat aatgttgctc aagcaatctg 41220
ggacactaaa cctgctggta ttagagctta tggggctatc tttgggacag cttatgactt 41280
aggtggacta tctcataata tttacttcag taggccaaca cctaagtatg cttttgtaaa 41340
agtatcatac tctctatata atgaagaaag cttgacaatt ccagaagaag atatcagaga 41400
cagtattatt caaggtatta acgcttatgg tagaactttg aaagttggta atgatgttat 41460
ccctaataga atctatgggt atatttataa tgtcattaaa ggtatcgaga ttaatgaaat 41520
caaagtagca ctatccaata gccagtcagt accacctagt gatggacagt acactacagc 41580
aagaattact gttgatggtg accaatacac tgtatgggaa agtagcaagt acaccattac 41640
taaggagtga taatgtttca gaaaattgat gatgtttatt acaagactct tgatgaaagg 41700
actgttacac agtttaaaga gaaattcatc tatacgagtt tgctgaaagc tatcactgat 41760
gagctacaaa cattggaaga tgtttcatgg cagatgcaca ctgaaaggaa tatcaggaag 41820
gctattggac agcaacttga caacatcggt gcattaatta aagtaccacg accacttggt 41880
gctgatgatg aaacatatag agcaattctt tatattcaga tttttctaag acgttctgat 41940
acaaccccaa catttttaca gaatgctatc atgactctgt atgatgcaac attttcacag 42000
ctttttgaac atattacacc tatgactgct ggtattgtgg ttagagttaa cacaagaaat 42060
aacgttgttg acacagcata cacattagca aaaattgctg caacaactat tggttcagca 42120
gtcattctaa gagatgtaac cctgaatggt actgcttgga cacctgtaga ggttgctgac 42180
tctgctttag caattgttga tgataaagat aactggcttg ttacagatgc taacaagggt 42240
ctggttacca acaatacagg aggttcttta gagaagaact tactagggag tttagcagat 42300
gctggtgtaa gagatgctta tttcaagatt gatagaacgg ctaatagtgg ctcagtagat 42360
tacttgaagg ttaataaaaa ttataatgct acagacaact acatcgttgg taaagaaact 42420
gtagcaggtg gtgattatgg tgttatggct gaagtagctc aaatcatcaa aggtagaaaa 42480
gataaatcac agcaagaagg aagttcttaa tggcattttt aaattggtct acagatgaag 42540
tagatgctga tggtaaccag ctaaaagtat taccaccacc agaaattcag gcgactggtt 42600
tattaagagg tgaaccaatg ggtcgccaat ggtttaatta tatcttaaac tatctcctga 42660
agaaagcaaa tggtactgtt ggtgaggtaa ggtcttttgc cactgcgcaa ccagatttgg 42720
tggctaatgg ctggaagctt attaagaccg aaacaggtac tgcatcaaca agtacaaaaa 42780
atctttatac ttatgaattt gtaggagctt aataatggca gtaggtgaaa ttcaaattag 42840
tgccttgcct caagcatcct taccaattga ccttagtgat atcttccatc ttaagcaggg 42900
tattgaggat aagaggtgta ctcttgagca attacttgct ccacacgcaa gcctaagaaa 42960
taaccctcat ggtgttacta aaacacaaat tggtttagat aatgttatta atgctcttca 43020
gttagtcgct gcaaataact tatcagatat tgttaatgta gctgaggcaa gagcaaatct 43080
acagattatg tcttcagaag aggttaataa ccttgtccag caacatatca aggacaagag 43140
taacccacac aatacaacta aggcacaggt tggtttaagt aatgttcaga actggacaac 43200
atctaatctg tataacgaag atgcagataa gtatgctaca gcaagagcag taaataactt 43260
gtacaaggct gttcaggctt cttatccagt aggtactatc catctttcta tgaactctgc 43320
aaacccttcc acatacttaa tttgtggagg tacttgggag ttagtttcaa aaggcagggc 43380
attggttggc tatgactctg atacaagacc agcaggttct gtatttggcg cttcaactgt 43440
cactttgtca gttaataaca tgcctcagca ctcccactct gtgtctctta gcggtggtgg 43500
tcacactcat aatgcaactg tgtcaatttc agcttttgat tatgggacta agactacaaa 43560
ctcttttgac tatgggacaa aagctactaa cacaactggt aaccatacac acagtgtgtc 43620
aggtaacact agcaccaatg gtactcacaa ccatgatgtg tatcctagag aaaatgatgg 43680
cactggtaat gctgtggctg actctgatgg tggtggtgca agaaggacat caaggacttc 43740
tagtgatggt gcgcactcac acagctttaa cgtcacatct ggtgcatcag gtgaccacag 43800
ccacacggtt ggtattggtg ctcacagtca tacagttggt attggttcac atacgcatag 43860
tggcactgta acagtttctt cttctgaaca tacacatagt ggcaatacaa atagtgttgg 43920
tggaggtcag gcagttaata ttgaacagcc atccttcgta ctgtatgtat ggcagagaac 43980
agcttaattt ctttacaggg gctttatagc cccttttaag aggaaaattt ataatggcag 44040
attacaaatt gagtgagcta aactcaatcg acacaatccg ttcagatgac cttcttcatg 44100
tcagagttaa aaagagacct aaaatgctgg gtgatgaaga ccgtcgaatg acctatcaag 44160
atttcttagc atcttttaag cttgaaagat ttgttcagat tgctggtagc actatgacgg 44220
gtgacttagg aattgttaag ctactttacg gtggcaaggc tgtattcgac cccacaggct 44280
cttctgagat tactgttggt gatgttttaa agacttttaa aatcaacgca aacggcctta 44340
aactaactat tgcagacgct tcaaggtcgg caactgttta tcatactttg aataagccaa 44400
gccctaatga acttgggatg agaactaatg aagagaatga cgcaaggtat gcaagactag 44460
ccgtgaataa tacctttaga ggtactcaag ctatcctctc tgacaatgaa gcgctcatta 44520
ttaaaaatat cactcaaggt gctccactct atcttcgtgg tcaagatgca gatggtacta 44580
acagatggta tctaggtaat gataataggg gtacaaataa tcttgtattg aagaatgtaa 44640
agtctgacgt ttcaattgct attttagaga atttggtgac agtcaacaaa acagtcagaa 44700
tcactggaca ggtgcaacct tctgattgga ctaatattga ttctcgatac attccagcag 44760
ctactttgag taatcttgct aaactaagtg atgtaaacac tttcaggtct gcacagatta 44820
ttaatcaaaa tggcttattg ttacagctaa agaatactac acaagatagc cagttatact 44880
ttgctggctt taatgctgat aatgttagaa ggtggtacat cggtaacggg gaacaaaaac 44940
cagaaagttt cgttatccac aatgatagga ctgctactac cattttcatg gtgaatgact 45000
tcttactgaa caaaacagtc agaatcactg gacaggtgca accttctgac ttctctaact 45060
tagatgctag atactatacc caatctgccg caaacgcaag atatatgctt gcaagcatca 45120
ctggttcggc ttctgaagca gatggtggtg atggtgttgc atgggatgca aaaacaggtc 45180
tttataaggt tactaacacc actggacagt cttcttcact tgtatatcac atgtttttag 45240
ggactggttc tacaccatct gcacaattaa agttttcgtt taaaaatggt gggatgtggt 45300
atagaacatc aagagatgcc tatggcttcg aagttaattg gtctaaagtt tacacggaag 45360
cacaaaaacc aaccccttca gagattggtg catacactaa agctgaaact gaccagaaaa 45420
ttgcaacagc aattagtgac tctacagacc ttaataaaat ctatccagta ggtattgtaa 45480
cgtggtttaa cagtaatgtt gaccctaata cagcacttcc tgggttaact tggacgtacc 45540
tgaacaatgg tgttggtaga actatcagaa ttgcagcagc aaacggttca gatgttgcta 45600
caactggtgg ttcagattct gtgacgttgt ctgttggtaa cttgccatca cacactcata 45660
gtttctctgc taccacttca tcttttgatt atggtacgaa gggaactaac agtactggtg 45720
gtcattctca ctctgtaagt ggtacaacaa gtgctgctgg caaccacgct caccacttag 45780
gtctgttatt agttaatggt ggtgatgctc agtatggtta cacaactgtt agcaacagca 45840
agacaagaac tcttgactgg cttgatagaa gagataacaa gttccctaac actaacacaa 45900
ctggtaacca tacacacacg tggtctggta ctacaagcgg tgctggtgac cattcccact 45960
ctgtaggtat tggtgctcat agccacacag ttagtggtaa cactggtagc acaggttctg 46020
gttcggcatt tagtgttact aaccagttct acaagttaat ggcttgggta agaactgctt 46080
aatcccttgt tgactgattg ttaagatggt gttaatattc tttatgggta ttctcaccat 46140
cttggctgat gaggtgatta aatgcctaca atcctagcaa ttcttctaaa aaatctaggt 46200
agcttcttct ggaaactcat tttatccctt ctaagtgaat acatgattga gaaagtgttc 46260
tttaagcttg caagatacct tgcgagtaaa acagacacac ctattgatga tgagttcgta 46320
gataacttag aaaaagcttt taagggggag aaataaatga agtggctaga ggaagctttt 46380
aaaaataata ttggtgcaat tgtagttggt atttttagtg ttattgggat gtttaccacg 46440
atgcaggttt caagtggtaa acaagaagtg tctgtcacaa caaagttaca gcagctagat 46500
aattattcca aaagtaacta ttcagctatt cgtgatttac agtctgacat gagactgatt 46560
cagctaggga tggagaacca gaaagttcag ttagagaacg ttaagggtga gaatgcaaaa 46620
cttaccaaaa ctcttgataa attctctgac agtgtgaata atctggctca atcagtatca 46680
gccttgcaag ctattactga aaagaacaca aagaatactg aaaaataaag tttaaggctc 46740
ctttttacgg gagccttttt tgttttactt gcctactgac agaggtgctt caatcttccc 46800
tgcatgttgg tagttgttaa ttccaccaat aaagtcactt gcagtaagat gttttaagtc 46860
acttaaagtg ttaagctgta caccaatctc aaaagtaggt gcatggaact cttcattgtt 46920
catcagttca taaacctgct tcatatggtt ctgataaatc tgagtatctc caaagacacc 46980
tatcaagtac cgtggagtgt atccagtcat cttacaaaga acttccagaa taaaaccata 47040
agacgcaata ttaaatggaa gccctaaaaa ggtgtctaca gaacgctgat accattgcaa 47100
gtcaacttca ccttcgttgg tgatataaat ctgaaacaga acatgacaag gtgctaaagc 47160
cattgagttt gctgcaatat ctgctgcatt ccaagcatta acaagcatgt aacggtctgt 47220
gatatcacct ttcatcttcg ttactaaggt ttctaactgg tctacaacac aaccattatg 47280
accttcaaag cttctccact gaaccccgta gattctccca cctgcatctt ctaaccagtc 47340
ttgctcagaa gaatagttag agcttaacca acgtttaaaa tcatctgacc agatagtcca 47400
acgttctcca tcattttcac cccaagtgcg ataacggagt tcaccaagtt tattctcacc 47460
attcaggaac cataaagctt ccccaatgac ttgccgtgta aacacctgtt tagatgttaa 47520
gagcgggaaa ccagttcgca tatcaaaacg aaactgaggt ggagcaaatg cagagataac 47580
atctccagtt cgtgtagtac gcagttcacc aacggataaa acatggttca ggatattttt 47640
gtaacttgaa tctgcttgtg acatatcaaa agtgtccttt tacttgtgga acataaattt 47700
caaaagttgc tttaccatcc tctgatggtt ttgactgtac ctttgtaaat acacggttat 47760
catacagctt ctcaaagaag ttttcaaaag gtaaatgcac agtggcctct tcagtaactt 47820
tatggaagac tgtatggaac accacaccag catacggtaa agcattgaca aggacacctg 47880
caccaccaat tacaaagaca tcttcatcaa tagagctatc aagatacttc aaaaatgctc 47940
caaatgactc tttactggct ttagcataca ttacatcatc tttgccaaag tctatgccta 48000
agtatggtac agaatttgtt aagattaggc ttgcacgttc tggtagaggt ttactaccca 48060
gagtcttaaa agtctcatta cccatgacta ctaagttatt tttagtgagt ctcttaaact 48120
cttgcatgtc ctgtttatgt cgaggccacg gcataccagt tggagtacca aactcaccat 48180
tttcaccact tgcaaagatt aatttaatca ttttgtaaat cctttcttaa tgaaccagtt 48240
gataacactc ttcacaactt tactacgatg gtagataaaa tgtggagagg gttggtatga 48300
accataagcc cagaaagcga taatttcatt acaccctgtg catcgggtac tttcttcagc 48360
aatatgaccg ttgactattt cacttacctc accttgtaag tctgtacacc cgcaatgtgg 48420
acaacggata ggtgttccat cagactcata gcagtgttca attttcatct atttaacctc 48480
aaaaactggt tcaagaacca atataaagtc tacacagtca tcttcttcat ccagaatacc 48540
atcaaagttc ttaggtacaa agtcgaagag gctcatagaa tatcggtcag aagtaaccct 48600
gatagtgttc cctatattct ctacagttaa tattgtacgg ctttctgtta cctcacttag 48660
aagattactc acaaagtcat ctaagaagtg tttcatcagt gacttgaagc ctttagggta 48720
atctgtacca gagtatgttt caatctgagg aacaagctta acatttatac tagtctttgt 48780
taagaacgtt ttagagaaca tgctatcagg tagcttacaa acagtctttc cagtaccttc 48840
atgttcaatc tcaacaaaac cagcttcatc gccacatgta actattttaa aagatgcttc 48900
attctcatac tgtgaagcca taaacttttt aagcatcgtt ttaaggaaat ctgcatgggc 48960
atcttgtaag aatatatcaa tcatagttaa tcacctatta tgggaacaga cacttgatgt 49020
catcgttttt gaaaacaaaa cttgtgtagt tatgaccatc aacatccagc caaacagcct 49080
gaacatctac accatcaaga ttgtggtaaa gctctttgat gaagtggtct ttcacaatct 49140
gactgctagt tgtttccagc ttaatgttcg agattgctga acctacagcc atatagccca 49200
tgacacgttt cttctcagta tgcttaatct taccatcttt gtaagccata atgagattgg 49260
agagaatctt cacaccataa tatttttcaa ggtgagtagg tacgccataa aagcctaatg 49320
cgaagttttc agagtggata atgttttcac gtttcatatt aatctgccaa ccttaaattg 49380
aaattaacac caaatttttc acatacttcc aacatatact caagggagac gttaccagtg 49440
agatggatta tgttggatac acgagcctga gagataccac aaattttagc aacttgctct 49500
tgagaaagac ctctggattt aatctctctc ttaaaacgat gtgcaacgaa gtctctcatt 49560
tcgtctacat ccattggaca aatataactc tccatttcag cctctctatc agcctcccaa 49620
tcatcttgtg gggcataagg gtcaaaaacc tcattcatct ttcttcttcc gtttgcgtga 49680
agtgtcaatt aatgagtcta tgtttgtagc cctaaccttt tcacgttcat cgttaagata 49740
atcgtcaaga gccttttcaa tctgtgaatc atcttgacag ataactgtaa agactgtaaa 49800
gccgtctgta tcgtcaatta tcttaccata accgcttctg ggatgtaact ttttaagagc 49860
catatcaaat acctgtgcag cttctgtttt ctaagaaata ctcaacacca tacatagctt 49920
tttgcatcaa atcttcttta aactggatac gatgattgag catagcctgt acatcaatat 49980
catcagggtg cttctcttta atatttgcag attgcaggaa atactctgca agaacacgat 50040
actgatagtc agttacctta tcagtcatct ctgttaaaga ctcttcagag attggtttaa 50100
gtagttcacc tttagatagg gcaacacctt taacaatctg acaagtgtct tctggaacac 50160
ctgataagtc cacatttgga agttctgttg cattgactgg taaattcatt gccataacca 50220
ttgcagcagc taataaaatc tttttcataa tgacctcaaa gttatattga attttgtatt 50280
acatttagta taaaaataag ctataacacc tgacttgtca acagatttta tagcttattt 50340
tggcagattt tttataaggc tactgccatc cttccatacc ttaccttacc acacctaacc 50400
atacctaacc aaactacacc atacaatacc ttgttaaaag accctcttaa agacctttta 50460
aaaaggagcc tccgaagagg ctacctagtc aacttagaaa ggttttctgc gttgcttgtt 50520
aatgttagct ttcatagcag ctaagtggtt ttgctgttca attagtcgtg aacgttcttg 50580
gtggctaagt gccatagtgt tcacattctc aattgcttga gtagcttgct ttaaagcacg 50640
accaatttta ccagacattt tacgttttgc aatagttgct tgatgcttag gttcaaccac 50700
acgataacca acacccattg aagcaaccag ataaatcttc tcttctttaa gaagcttctc 50760
aataaacttc tcaagtcggt ttagacgtgt aagagcataa gccttcattt catcttcgta 50820
cttaacaatg tcaccttgat aagttggctt cacaatacct aagaaggtgt ccatctcaga 50880
gtgtgataca gtgtcaccat aactaaactt tgctgctaat gcttttgtat tcataattaa 50940
acgttacctt ctgaaataac ttctacagaa aaacgaccaa agcgtggacg ccaatcacct 51000
acaccacaaa ggttgccagc attttccaga accatcagga gttcttcttt ggtgatttgc 51060
tcttcatcga acatcaaacc aaactctaca gaccagtcac ggaagattgg acggtagctc 51120
ataactcgtg ctgtgccaat cttaacagat ttagcataga taaagtctgg attcttagca 51180
agttcttgtg ggtttgctgg gcagttcttg atagtcattg ggaagactac atctgtcagc 51240
ataatggctc ggtcaatcac cttaccaagt ttgttgagtt ttgcaccaga cttgatacaa 51300
gcctcaatca tctcaccgtt cataacaaaa cctagctgct catcgtagta acaagaggtt 51360
accagttgac tttctgctaa gagcgcatag tcttcgtcag tctttttacg tttactagac 51420
agagacttgt gatattttgt cagagggttt aatgggtctg acagtgtatc agcgtggctc 51480
aggaaagggc gagtaccagt gattttgata tttaacagtt tcatagcatt tagctccaca 51540
ctttagttga ttgttaagtt gtttgtaaag aaccctcaac agaagactct ttagaaactt 51600
ggtagatttt taacgtggct actaccatcc acccatacct tacctaacca aaccgaacct 51660
cacacaacca gaccgagccg caccccgcct tattaaaaga ccctcttcaa agacctttta 51720
aaaaggtaag tctcatttta atgtagctga gactaactac ccatactgta ccgcacctga 51780
cccgacctga ccagaccaga cctaacatta ccgtgccaca ccgtaccgaa cagaacactg 51840
ccatacctca catcacttaa aaaccctctt agaagactct taagtgatgc tggtgagtac 51900
ccgttggttt atgctgctca gtatactcac caaattttct atgtcaacaa ctttttatat 51960
aaaagtcctg atagctatat gtgttacaaa acagaagatt gtgaacaaaa cacccattgc 52020
aaaagatgtg tccaaaagct tacttgcaat gtatgacaat acaaagacaa ccagtataaa 52080
cagaatgact gcactgaaca tagtttactc ataatcccta atagctagcc cgacagggaa 52140
ctgtaaagaa cccttacgag tcatcttctg gaactgaacc gttaaaggct tcccgataaa 52200
ctcttcagga tgctcagcaa gatactgttt cttctcatga gtagtcttcc atgagacatc 52260
tacaaagacg ttaggaagag tctctacaac gaacttgcca tgaccacgct tatcagtctt 52320
tacaccagtc actttaaact cttcagtgtg catcttcttg tgcttaatca agaagtatga 52380
acggtgacaa cactcataga aggagtcttc agagattgaa cggtacattg caccttcaaa 52440
cttagcttca acccacttat cgtgagcttc atcaaactct tcccaagaat ttacacgacg 52500
agacttaaca ggaacaactt tacagccgtc tctaaagtca ttcaatggtg aagtctcaat 52560
aatatctcta cgctcaggcc acgtcttagt actgtcacag atatcgtacc agtaaaactt 52620
gagcaagtgt ctgtcaggat tgtcttcatt tttaatcatg gagacgatat cttctaagtc 52680
ccacccatga gcataaatct ctccatcaaa ttcatcaaca tctttgtgga gccttaacat 52740
tgccataatg tcaggtatta gttcagtagg gattttgtaa acagtattct cacgagaata 52800
tgctgtaaat acactgccaa tactcaagtg ccttgaaatc ctacaacgga caccatcaag 52860
tttcggttga gcatcagcag ggaacttcag atactttgca tggctaacct ttgcagcatc 52920
atgagcaagc tgtacaccta ccttctcagt attctgtgca gaatcttttg tgtaagcata 52980
acctttgcgg tcaacttgct ttttatactt agcagcaact tcaaagagag cttgctgttc 53040
agcattacgc tcattctttt taccgatgtt cttaggctct gctgtgtact cttcaaacat 53100
catcttgcca ttttctttac cataggttgt gataactttg tcacctacag caacacatga 53160
ccagacgttg aaggaaccat ctttattttg tttgtacaga attgtcattt ttaacctctg 53220
agtgatacca agtgttgcag gcattacatg cgtaaagctc taagtcgttt ttatctggtt 53280
tatggaaaca gtttgaccca caacgacacc ggaataactt accatcaaca cgaagaatga 53340
atgtttcaag ctcaccatct tctgtaagaa ctgcgttctg aaatttatta tctttaccca 53400
tttgtactct cgcctgtgtc agattttata taagacctct cactacgtct tttaatgaaa 53460
tgtacaacgc attttggtgt attttttaca tctttaatgt gcaacctttc gaaactgtgt 53520
actgtgaaga ttgcatctgt cattgtttgc ttagatttca ttttctctta ccaccaattt 53580
ccacgtcgat aacttgaaca tcaccagcaa catcaaactt atgaatgatg ctttccacag 53640
tgtaagtcca accctcaatg cgaaccaatt caccttctcg cggtacgatt gggttacgtt 53700
gggccacggt tgcagtggaa taaacaacat cgtagcagta gatatagtgt actaaaactt 53760
gcatattagt ctcatccatc aataacagta gtgtgtgatt gaccattttt gccatatcta 53820
taagatgttg ttgtttgaga gtttgggcat ctgacaacaa caatatccct gcttatagca 53880
tccccattga gtttgtagat tttgcagtct tgcatctctg gagggaggct gtaagaggca 53940
tctttgtttt cataagatgg gttacagcca gccagaaaaa ttactccaag tgcaaccatt 54000
gctttcacac aactcttaac cttattgttc acgataatct acctctctgt atgtttttaa 54060
atagtgagtg atgagttctg gtaattcact acgtttaacg tttgaacgaa gaagtgtcca 54120
gtcaaaacct ttaccaaccc tcccccagag actgtacatt ttcttcatag caactccaga 54180
atatcaatac gttcaccttt gagtactcca gtacggtctt ttgcaatctt cccttcaaca 54240
ataagaatgt gtgcagacct taaagtgtca ctgttaaagg cttttacgaa cttgctaggc 54300
atcactacaa tacacatctc ctgaatcaac tctggagtat agcagttgtc gtgaagttgc 54360
tgaataacgt ctgcaagtgt catttttgat gcctccaata ctcaatatca ataagttctt 54420
gtgcaatgct tttaaggtta cctttcaaga aaaccttctc agaacctgaa agagatgaat 54480
tgtcaattaa cctgttcatc ctctcaaaca acttctgctt acgattcttg atagtctctt 54540
ttgaagcacc tttcatcatc gctgcatggg ttcttccacg gtggctcata agccctccta 54600
tataagatat agtataaggt actcttccat gagcaccttg tcaacttagt tttgcaaact 54660
ctccgtggag tttaatagca gcttcacagt aagctttatg ggcttcttca ggtgtgtcaa 54720
acataccaag atgatagttt ttaccttgta aacgtatcat tgctcgaaac cttttactta 54780
tcttctgtac accttttggc aacccagatg gggactctct tgaccgattg caagagtttt 54840
gtgaagctgt acaaaccctt aaattcaagt acctattgtc ccaaccgtct ccattcttat 54900
ggtcaacctg taaggtttta tcaggtagat atccatccat atacaagata actaaacgat 54960
ttgcttggta accccttcca tcaatcagca actgaactga cttgtgtcca tcagtatttg 55020
ttctgtagtt tccagccctc ttacctccaa atctagagtt aaagtttgca ttaccttctc 55080
taaacttcca agtaaagacc cctgtttcgg ggtcgtagtc caatagctcc ttaagacgaa 55140
tttctgtaat catttcaaca ccatcaatgt acttccaacc agctcttacc aaccttgcct 55200
gtgccagtta caagcgtctc actcttcaaa cctaaattct tagatgcctg accatacatc 55260
cagtcagtta tggctttaac ttcttgagtc atactttctg ggcattccca actgttttca 55320
tcgtggtatg ccagcaaaag cctagcacca attgctggtt ttcgtccatt taattttgtc 55380
aacccttctt cacatgctct tctacaagca aggttaattg cctcattttg aatctgagcc 55440
tctgaaccca tgagcaaata attcaacagc ttatgcggag atttacacca caaccaagct 55500
ccagcaacct ttacaaaacc ccctttagag attgcagcag tcttaccaaa cacctcttca 55560
agtgcctttt tagttgcttt gtagtcagct tccaagtcat ctagcagctt tttaatttta 55620
ggcaaacgaa taaagtaagt ctgtttagtc agcgcacctt cctgagtagt tgcagcttta 55680
atcgtcttag caaacttctc atcacctgca ccgaacagca gtgcatagat accattctta 55740
gctttcttac gacccttgct aatctcatga agaagattct catcctgagt agctcgacaa 55800
gtcaagatgt cctgctcatc gttcaagtta aagtaaatac tgttcagtgt atgtgcatca 55860
gtccctgaat agacaacgta caggtcattc tcagagtcat agcgaaggta cttatcaatc 55920
tctgggttga ggtactcatc aaagtgttta cagtaatagc gtccatcttc ttgacgaatg 55980
aactcaattt cttctttacc ttgcgttacc gctttggtga agtctttatc acccataaag 56040
ttacaaagaa gaactagctg ggctgagttc atgtctacag agacaacgtt agtaccttcc 56100
tcacaaatcc acactgccct cattggttcc ccatagacag cagcaccaga cggtacgttt 56160
acaataccat actgtgtcat acgtccagtt gaagtaccaa acaccattgc accagcacta 56220
aggcgaccat caggacgaat ctggttcaac caaccttttt catcatcctt tgagttctca 56280
atagttctgc gtcggtgcat caaagtgtag tatttagcaa tcttctgtcc aagctcacct 56340
tcaatcgtat cgtaagatga ttcagtaagt tttggtgaag tacggattaa gcaaggctca 56400
agcaaatctg tgtacttctt cacagaccag ttatgctcaa tgtactggac gccttcgtgt 56460
tcaacataac tcaaaccaca ccgctcaacc atttcctgcc acttaggatg ctttgtaatc 56520
atctttttat tgtctttgaa acgacaaact ttgacagggc gaccatcaga gtctttcttg 56580
tagttccagt catctggaat ccaaccaact gatttcaagt agtctttaac aactgctacc 56640
tgagtcatac gagaaacttc aaactcaatt ggtgtatatg gtgcatcaat caaacctgta 56700
tagcgacttg actcaagttc aaagtggtta acaacatgac tgttgtagta cttcacagtt 56760
ttgactgttt ttactggctt ccagtctttg catttcttac caatctttgc attcaactcg 56820
ttgcaaattg cacgagcatc cttcattgct acaaaacctt ccttgtactc ttcaccagtt 56880
acagagttgg ttggtgtata gcaatttctc ttttcaatgt taaaaatctt tgtagttggc 56940
ttaccaaatg gcttgatttc atatgtctgc atctcaccgt tacgtacctg ctgacggtac 57000
tttgtcttag ggtacttggt aattctcttc agtccatctg catgaccaaa tgtttcaaca 57060
tactcattcc aagcttttgc aaactcttct ccagtgactt tacctttggt cttaatagtt 57120
ggaggtagat gcggttcaac ctctgaagca agctcattag tcaacttgtc aagttccttt 57180
acatggaact ccataagctc tttatcagct ttccaaccat tgatagcttg ttgactcatc 57240
cagaaagatg tttctttagc tcgcatgtag gtttcataag tgtctatgcc acacttctta 57300
agcttgagat actcattatc aagtgcccgt ttagttttgg cgttaatacg gatatcttct 57360
acaacacgag tgaagatttc tgcattccac acaccccaat gttcaatctc tggtttacga 57420
acaccaacac gagcacccca tgcagccaag ccatgagcac ctttataacc ctttggagtt 57480
ggtctatcca tccactgaac acgagactgg ataagagaat cctgaaagaa attactccac 57540
ggcttacact ttggattatc aaagttccat aaatccggtg caatgtgatt gaaaacccac 57600
cagtcataac ctagaccatt atgaatgcaa agacgttttg cctttaacgc aaactcaata 57660
ccttcacgta agccaccctt aatgtacttg gtatacttgt gaccgaggat aggttcatct 57720
gtaaagaccc atacaggagg ctcttcatca tcagatttat agtctgcaaa agccattacg 57780
tgaactttag tgaactcaag gagtaaacca tcagtttctg tatcaccaac caaatgtaag 57840
tttttaaaat ctacgttttc catttttact ccctctaaaa tcattaacct catacattac 57900
tgcaaaaaat tataaaaagc aagttgacaa tgcacttgac aaggtgttaa tctttgcgaa 57960
gtgggttttt acttaaaagg ttacttaaca gtttaactat acagatactt taaaagtctt 58020
aaaagattat ttaatagctt ttaaaaagct ttaaagtata acgtatagaa tacgttaaga 58080
taaaagatta aaacttaata gttacttaaa agaggcttac atgcagaaaa tgtttgttca 58140
cccagacata gctgactttg ttagagcttt gcaaaagttg gaagaacttg acattgctgc 58200
tcaacgggaa tatgctcacc atcacaggag aatggttgat atacaacatg aaatagagtt 58260
gtctgaaaac tatgaagatg acttaaaatg ctctgtattt gagcatatga aagatgttgc 58320
tacagcgaga cgtaaagcta aagacacagt tgctttacta gattctctta aaaagaggtt 58380
gcaaagtggt acagacctgt gtaatctagc gtcactgatt aacgatgttg aaacttcttg 58440
ggataggcac tattatccac gttctgaaaa gacacttgac ttttcttcat ccgaaaactt 58500
aaaatgttct agaaagaaac ttaaccaact gagagagaaa taatatgaat atcgatgtag 58560
aaaagatgct tgaagagaaa ggtattgacg aagcattttt aatcaatgcc gtagaaagca 58620
tggctatgat tcttcgtggt aacaactaca cagaacagtt tatgcctgca agctataact 58680
tgcctgaaga caagtctgat aacggtgctt ttgcagaaca ggttaacatg atgctttgcg 58740
acaaaacact tgctttagca tctatcgttg taggtgttga tgcccttgca gagttcattt 58800
tttatgagat gcagaaaaat gattatattg ccatgaccac tgaagaaaaa gacttgctac 58860
tactcgcagg taacgatgaa ctaattgacc tgttaatttc atcaactagt gctgttatgg 58920
ctgctcttga gaagcgcatg gaagaaaaac tttcacgata tgaaaaactt gattggggtg 58980
tagaagaatt aaacccacga atcaaagaaa ttatccagca aactaaagag tctttggaag 59040
aagctgttga aaatgactct gtagctgata tagaacctct tcaggtgaaa gttgcactga 59100
tgattccagc tatctctacg atgatgaata tcgttgcact aatccaactt tcacagatga 59160
tgggtgtaag tattgagttc attgaagaga tgactggtgg cgttagtatg caagcaatca 59220
atgatgtttt ggtgaacatt ggtgcttcaa tcattgaaga gaaactacgc attaacttcg 59280
gtgatgattt tgtgaagcaa atctcagcta tggctgaaaa agagtatgct gagcaactgc 59340
actaagtctt tataaatctt acaagcctcc ttagttgggg gctttttagt aattaatgga 59400
gataaaatga gtaatattat cgcatttact ggaaaggcac gttctggaaa agacacctca 59460
tgttctattg tgaagaacat cttagaggat gagcacggct acaatgttgc tgtaatggcc 59520
tttgcagaca accttaaact gtctgcatca aagatatttg acctgacttg gaatgacctg 59580
tatggtgaaa ctaaagagac tccacaggta tttgacttgt catatcctga actgatgttt 59640
aaagttacgg aagctatgga gttcaccttc agagatgaac gttaccatat ggacttcaag 59700
cttatgtcag agttaaccgg acgattaatc atggagctta agaaggttgc taaaccaact 59760
ctattgacac gtctggggtt tagcaaaaag tataaattct catcaagaca aattcaacag 59820
atttggggca ctgaagttgt ccgtaaagta atgggagaca agttctggtc taaagacctt 59880
gaaaaaagaa tggttagatt ctatgaaagt tgctcactta gaaatcaata tggttttgtc 59940
ttaatcagtg atttaagatt tgactctgaa gctgagtggc tgagtagatt tgcacaccaa 60000
actattgaag taaaaagaga caatgtggat aagatttcat cacacgtttc agaaaatggg 60060
ctatccacca aatatgagcg tgacattatt cataataatg gtactcttac agaccttgag 60120
aacaagctaa gagccatcat taaagtttaa aagagagaat gaagatgaga gtaaaagaca 60180
attttaaagt tattgaccac cgtttagtgg aactctcatc tctttccaat gaggtaatga 60240
ttgaacgtct taagagagtt gaatcacgaa ggaaagagat tgcagatgaa attcatgaac 60300
tggacaagat tgaaaatgga ttgaaggcag aactacaacg aagaggtgct aatgtctaaa 60360
ggtcgtaaat tgaaagaggc tggtcagttt gttggtcatt gtgcatgtcc acgttgtggt 60420
tcatcagtgg ctggttcaat ctatcatcat gacgatgatt cttattcgat gacttgcttt 60480
agttgtaaca aaggtttccc agagtgggat tttgataaag gacaaatcgt gagcacttat 60540
tctactggtt cagacaataa aaaccgtact ttccgtggga tggatttaga cgatgtaaaa 60600
gacaaccttg aagcaatgga cttaaaggac aggaagattc ctgcaaaagt tcttgagcgt 60660
ttgggtatca aggttgacat tgatagtgac ggtgaaattg atgcgcattt ctacccaact 60720
tacaaacgca atgaagatgg caagctagag catgttggct accgtgttcg tcaccgttac 60780
ccagaagacc atccaaaaga gcacctacgc ggtaagttaa aagacttttc tggtggtgtt 60840
ggagatatta aaggtgaact ggcaatgttc ggttcatgga ttgctccaga aggtggtaac 60900
cgcctattca tctgggaagg tgagatggaa tgtgcgacag caatctatat gacttctctt 60960
gcgattaagg acaagtctcg tcgtaagaat tactgtcacg tatctgttcc atcaggtgca 61020
aacatcaagt ctatcaaaga taactatcag tacattacat catttgatga aatttacttg 61080
tgctttgata acgatgaagc aggtgctaaa gccactaaag aggctgctgg tatcctacct 61140
attgagaagg ttcgtttatt ccagtatcca gaaggtgtaa aagaccttaa tgaatggtgg 61200
acaaagttct ataaagagaa agatacagtt ctggaaggat ttaagcagcg tatctacaat 61260
gcacctcgtt actgccctgc tggtatcaag aactttgcag acggttttga ggcaatgaag 61320
aatcgtggtc agattccatt gattcctttt ccagaatctt tcggagactt gaacaggcta 61380
acttacggtg gttacggttt aggtgagatt acgactattg cagcaccatc ttcagtaggt 61440
aagtcagctt acactcgtga gatgatttat tcagcttgga aagaaactga ttataatatc 61500
ggtgtaattc ctgtagaaga tacctatgaa gagttgatgg aaatgctctg tgcaatccac 61560
ctgagcaaac agatttctga gattccttat gatgaacggg attgggatga attaaaggga 61620
gcacacgcag agctttcaaa aggtcgtcgc atccatatcg ttgaccatca aggggcgatt 61680
gaccaagata acctgctgga gtttgttgac tatcttgtta acagtttaga ctgtaagatt 61740
attattcttg accctattac attggctctg tcacgttctg atacggatga agaggaagtc 61800
ttgtctgaac tattgcgtcg ttgcaaacgc taccagtatg cacaggtaaa cgtttgtcac 61860
gttcgtaaga gtgcaggtgg tcagaaagct aactctgaag gtggagatat ctctgaagag 61920
gatattaagg gttctggtgc gtatttccag atttccatga acaacattct gttaatgcgt 61980
aacaaggttg acccagaccc tgttaagaaa aacttgacaa aaatcaagtt aactaagtgt 62040
cgtcgtcatg gtaagtcaac aggtattgct ggtcatactt ggtacaatcc agacacagga 62100
cgtcttatca aagcttctgg atgtggtgtt gacattgatg gtgcagcaga aaatattcgt 62160
caacagtttg gtattggtga agctgaagac cattatgacg actctttacc tcattatgag 62220
gatgaagtgt ttgaccgtga gactggtgaa gtctatactg aagaacagcg tcaaagctca 62280
acgattccac ctgtattaag tgaggatgca gatgactgcc cgtttgaaac tgagtgatag 62340
ttacaaaaga gaggggttca tacccttctt tgaagaaaaa agtttaaatg aatttcagga 62400
agagtgtttg acaaaggttg ataggttcta tagaatgctc tacatgaaat gtgatggagg 62460
cgttgaggta gaagaaaagt tccatcacag tatgataaca gtcactatta ctatacctcg 62520
acataatatt ggttggctgt ttataattaa agaaaatgtg tttgaatatc aagtatatcg 62580
gagaatatca tgaaacactc taaagcattt gaacaagttt ttggggattc cttaaaagcc 62640
actgctggta aaccagcaaa atactatgaa gagaagcgtg taagaactgg aaaaactgca 62700
cgtaaagcag cttctaaaga taagcacaac ttccagtaat taatgtttga caatagagta 62760
tcaacaaatt agaattggta ctcttcataa attgagatag aggtttaaat aatgtctaaa 62820
gttgttaaaa tgaaagctcc ggtagagaag tacaatggta ctgaacgtca aactctgcgt 62880
taccttctga aagatgtttg gttttattac ctgaacactt caccacgtcc gggaaaaggt 62940
aaatccattg ataagaaatt cccaggcaaa gactgcaact acagtgtttc aattctggca 63000
gaagatggta acaagctgtt taaagagttt actaagtcta agaaaaaccc agaaggttgg 63060
gataaagtta ctactgaagc agttgatgca gatgacttcg aagagaagtt tggttgtaaa 63120
ccaccttttg aagcagacac ttactacatc ttgaaagtaa gtcgtgcagc agcttataaa 63180
gatggtgctg tatggacagc taaacagtca ttccctgtaa tgctgattga agaagtaaat 63240
ggtaagcgtg tagctgttaa acagccgatg aagaaaatca aagctcaagc atccgataag 63300
catgaagatg acaagaacta tgatgtaatt catccagata ttgcagttgg taacggttct 63360
tttggtagtg tgattctttc tactcacttc tacacttttg agaacaatgt tctgacaaaa 63420
cctattcagg aacagtttat cattgatact cttgtacctt acactggtgg taacggtgct 63480
aacggtgaac ctgaactgga tgaagacgaa ctggctatgc ttggtcttga tggtgttgaa 63540
gataatggtg aaatcactga agaagacgca acagaccaca aaccttcgaa caattctgat 63600
gatggtgacg atgaagactt gccagaccca gatgacgaag aagatgaaga cttcgataca 63660
gaagactaat ctctaaaagt tactttaaag ccctgtactt agtatggggc tttttcatat 63720
ggagagtcac aatggagaag tatacactaa caaaacttcc cgattcagtt acacatgtct 63780
ttattgactc tgatagcatt gcctataaag gtgcttgtgt agttgagaaa gcaaaatata 63840
aatatgtcaa taaactcaca gcagaagaat ctgaaccatt tgataatgca aaagatgctg 63900
caagatggtt agcagaccag agaatccttg tggaagagct tggcctgaca tttgatgaag 63960
atgaatggga aagacagact tggaaagaag ctaagagtga aaaagaagct atcatggcta 64020
ctcagcaggt tcttcaggaa tggcttaagg ttgttggtaa agaaagaact tgggtaggct 64080
acttaacaga gaagggtgta cataaacata aagacattaa aggtcttgag caccaatatc 64140
aaggtaaccg taaagatgct gtcacaccaa cacacttagt tgcttgtcgt gaatatcttc 64200
tgtcaagacc agagttcaag ctcttaaagg gaggcttcga agccgattcc atcgtaattg 64260
ctaaagctga aaagatgggt aagaaggctg ctcttatgag tattgacaaa gaccttcgac 64320
aagctgaagg gacttattgt attgatatga cttatgaaaa gtcaccacta atttttattg 64380
ctgataataa tgttggtgat atctgggatt gtcctatcaa atcaaaacca aaagctgcaa 64440
aaacagttgg tgtaggtttt aaatttttgt gttatcaggc tgttgctgga gataatgcag 64500
acaactactt cggccttaaa ggtgctggca aagtagctgt gatgaatgct ttagatggta 64560
aagagaccta taaagagtgt cttgatgcta tctatgaact ctatgctaaa aaagagtctt 64620
acacctatgt gtcatgggat ggacagactg ttaccaaaac accattagag ttaatggaac 64680
aacatttctg ggtagcatat caagagagaa atccaaaaga taatttctct tttaagaaat 64740
ataattggga gccgtgtatg aatgaagatt aaaggatttg gcaattgccc cacctatggt 64800
cattgggtat ctttgtgtgg ggaagttgac cccactaaac attttggctt tgtctattta 64860
gtatactgta agaaaaccgg acaatattat atcggaaaga aacagcttaa tagtgtgacc 64920
aaaagaaaag ttgctggcaa gactcggaag aaggtagtca ctaaggaaag tgattggatg 64980
acttatgaga cttcttctga gtatattaaa aaagatattg agagctttgg aaaagaattt 65040
tttgactttt atattattca aacctaccac acaaaaggtg gtctagttta tggtgaagca 65100
aaccttcaac ataaattcga tgtaatgaca aaaaggattg actctaagct cagactcttc 65160
tacaatgcca atattgcagc aattaagttt attactaaag aaacttatga agatgctgaa 65220
aaaagaatcc ataaggtaat gaaagcaaac tgtgcttgat aactattgag agagaataaa 65280
atgtttaaca aaagtaaagc tgtgagtcat gtagcaaaag ttgatagcaa gattgaagaa 65340
cttgagcgca ttcttgcaaa tgccagagag tcaataatta aagaggttga agctgttgaa 65400
tctcaaatgc agcacctaat gcttaaacgt caagagctac gtgagcatct ggaatatatt 65460
gaaacacgag agctaaaggt taacagatat tttaaggagt cagaatgttt aactcaagag 65520
aatcagtaaa aaactggaat cttcgttgtg gaaacactca aaagcaaccc tacagcaatg 65580
agtattggga atctttgaaa tctcaatctc tatgtatgct tgaagaagca aaagagcttg 65640
taaaagcaat tgaagagaaa gaccccattg agacactgga tgctcaggca gatttgcaat 65700
atgttcttga cggtttgatt tacctgtcac aacatgacca taatggtgct atggaagctg 65760
tttgccataa caatgaccta aaatacacag acaactatga agaagcttta aaacgtcttg 65820
cagatattga gaagcgtact ggtcaagagt gtatcatcag aatgtcagta gttgatggca 65880
aagaatggta tgcaattgtt cgtgcagccg atgggaagat tatgaagcaa tctaatctcc 65940
ctaaagttca gcttggtgaa tacattgtag aacttgaaag ccaagaactt tttgtggtag 66000
tatctgagac atgcgttatc tgcaaaggta ttgtatgtag cttaaaggat ttgggtgtag 66060
atggttttgt agaagttaat ccaattacct ctaaagcaga taaagatttc tgtaaagaga 66120
atggactatg gattgcagat attgtctact atgatggtga gcagttccat gtaacctcat 66180
acccaaaact gaattatgat gctaataacc tgaagtgctg gttaaaaggg gttggttata 66240
atggattcac agaacattaa taaagaaggt gtggctcaga agagccactt ctcaaactac 66300
aatatctcta tgacggtgtt tatgaatgac ccgttacttg agaagtatgg tgagactcca 66360
gatacacttc tggataatga acaagtttta aaagcagttc tgtacaaata cgggattgat 66420
attgagaaag agtattcttt tgaaatctgt caacacagga atacttttgg taaagttgta 66480
atggctccac tcttcatggg tgtagaaaga actgactatg gttggttata tctaaaaaga 66540
aacttggaga aataccgtgt ctaaagcaaa aaagctatct tatgatgaca ttatctcagg 66600
tgctaaatta ggtgttgata gtatcggaca agatgttaag cacggggaca cagtcatgta 66660
ctgtgacgac agaagaggca gaagtgcaat cttgtttgga agagttgtgt gcaagatgcg 66720
aggtaattac gttgttgaag acatgaatgt ggatgtcaca aaacagcttg aaataattat 66780
gaatagtgat atatcatggt tcgtgaattg tatgcacacc tcttcagtca caaaggtaag 66840
tgataagttt tacgatatgt ggcagaatga gcaaattttc aagatttaaa ctagggagcc 66900
tcttcggagg ctcttttcat ctgtaggatt caaaaatgat taagacaatt aaaaaatcaa 66960
acggtacagt agtaagcttt gacccagaaa gactgaataa gtgggcatca tgggcagata 67020
agcgtggaat catctggtca gaagtcacta tggaagccat gaaacgtgtc tatggaggtt 67080
gcactacaaa agagatgcac caagctatga ttgatgtttg tgttgataaa caaactcaag 67140
agtactcaga catggctgga cggctacttc taggaattat ctacaaagaa gcctttggag 67200
gctttactaa ggttcctacg ctggttacct tcgttaaaaa tatggagaga gcaggacttt 67260
gggagaagat ggattactca caggaagagc ttgaatacct gcaaggttac atcgtgcact 67320
caaaagatat ctcttacggt tatgcagtct tgaaacagtt cagagacaag tatggtatcc 67380
gtgatattaa aacgggaaga ctttttgagt caccacaatt tatgtttatg ggtatggcta 67440
tgaaagcctt tgagaagcaa ccaaagcacc gtagactgca agatgttatc aagctgtaca 67500
cttacctatc tgacctgaag attaacgctc ctacgcctta tttaaacggt ttgagggcaa 67560
ctaaatcagg ttatgcgtca tgctgcttga ttaaggcaaa cgatactgct gaatcacttg 67620
gtattgctgc aaaggttgct tatgatatga ctacaaagca agctggtatc ggtatgctga 67680
tggagactcg cactattggt gatggtatcc gtcaaaacac tatcgaacac atgggtaaac 67740
tgccttatta caagcttgta cgttcatctg tagaggcaaa caaacagaag agtcgtggtg 67800
gttcagctaa taacttctac actgctctag acccgcagat tgaagattta ctgcgtttga 67860
agcaccctac aacggttcct tctaaacgta ttaatgagat ggactactca ttcggcacaa 67920
acgattattt ctggcagtgt gttcagtatg atacagattg gttgctattc tcttacaaag 67980
atgcaccaaa actctatgac atgttctaca cagcatctgc tgatgagttt gctatggcag 68040
ttggtcacgc agtacattca ggagttaagc acagacgagt caaggctcgt gaaattgcta 68100
agctgtttat tcaacagcgt tatgctacag gtcgtgtgta tccattcttc actaacaacg 68160
caaacacaca tacaccattt aaagaaccat tgaagatgtc aaatctttgt atggaaattg 68220
tgttgccagt gtatggtttt gagaaagaga cagaccttta cagagacgat gctgtgaaag 68280
aggatggtga ggtggctctt tgcttcctag ctagtttggt tgcagggaga atttcagaag 68340
atgaatacgc tgacgttgct tattatgctc ttgcaatggt tgactccgtt attgacctta 68400
tggattatcc gtatccatcg atgcgcaacc atgttcagaa gcgtcgttct gttgggattg 68460
gccttacaaa tgtggctcat taccttgcga agaactacgt gaactactct tcacgagcag 68520
gtaaaacgaa acttcatgaa ctggcagaga tgcactctta ctatctgcat gaagcctctc 68580
tgagacttgc taaagagcgt ggcgttccag agtacatgaa gttcactaag tatcctgaag 68640
gctgggttcc tccaaagaca gctaacaaga agattgatga aaagcatgat gcaaaactac 68700
gatatgattg ggatgactta gcacaacgta tcaaagaaaa tggtggaatc cgaaactctg 68760
tattagaagc ttacatgcct aatgagagtt cttcactggc aactaatacg acaaatggct 68820
tgtacccaat tcgtgacttt attttaacta aaaagtctgc aactggtaac gtgctgttta 68880
ttgttccaga ttatgaagag ttgaagtatg tatatgaaat tgcttggggt attgacacct 68940
ttgacctgat tgattgttat gcaattgttc aaaagttcac tggtcaagct atctcttcag 69000
atttctatgt tgactacgca aaagctaaga aggtatcatt ggctcaagct ttgaagtaca 69060
tgatttatgc caactcggta ggtatgaaaa ccatgtacta ccttaacagt cgcattggtg 69120
taggtaaatc tgcactgcaa gatgcttatt gcgagggttg tggtgtttag ttttaataac 69180
tatgagggtc gtagaagacc ctctaaaaat aactttggag agaccatgaa agatttaata 69240
gaaaaacatg agcgaccaat atacttgctc tacaaacccc gcaaaacatt gtactatgtg 69300
agcagcacag gtatcatgat aaagcagaat gatgacttat gggtcactgg agcctcttac 69360
atctctacag cagatggtaa aatctacgca agaccttatg agatgtttaa caaagagaat 69420
tgggaagttt tagacagaaa acaagcctta gaaatgataa agaaaggaga aatcacgcta 69480
tgattaacca gcacccaatc tttttaggtg gtgagagaaa aacttttgac tcactcaata 69540
agcactaccc aaaaatcttt gaactttata aacaacaaaa agcacaagac tggtcagaag 69600
atgagttccc ttttgaacaa tcacgtcttg attttgagag tgtcccagca tcaatgtcag 69660
gtgtaatgct tgagattctt aagtggcagt gggaagcaga cactcaagtt gctaagagct 69720
tggcatttgc ctttgcaccc tttatctctg atgacatcta tgcaactgca attatgaagc 69780
agtctgagat tgaaaaccta catgctctta cttactcaga gattgtaaga caatgtatta 69840
aaaaccctga aacaatctta gatgagatta accagaatgt tgctgtacaa gaccgattaa 69900
aaactgtgaa tcgtgttctt gaagaattac tggatgaagg tataaactat cgtctgagtt 69960
atgtgcgtga ctcacttctg gacaaagacc ctttacactt ccataaagtg attctaaaag 70020
ggttgtttgc agtgactgcg cttgaaggta tatcttttat ggcatccttt gcatgtactt 70080
ttgcacttga tgctcaagat aaatttcagg gtattgctca agctgtccag aaaattatgc 70140
ttgacgaaat ccttcacact aaaattgata ttgaagtttt aaaagaaact ttaagagatg 70200
atgagtggca gaaagctttt caacaaattc ttccagagat taaagtaatc ttagatgaag 70260
tagttgaaag tgaagagaaa tggtcgtatt atatcttctc cgaaggacgt gctgtagttg 70320
gcttaaatac aaagcttctt catgagtggg tttactataa tgctgcccca ctgtatgata 70380
tgtttggcat tcccagagat tttgtagctc ctaaagaacc acctttgaag tatatgatta 70440
agaagatgga aattgataaa gagcagaatg ctaatcagga gcaacagaac ggtgcgtacc 70500
tattgaatac tgttgtagat gatttgaata gtggattttt agaggttcct taatgactta 70560
tgtaatttac tccaaaactg gatgccctca gtgtgagact gcaaagaatt ttgcaaaagc 70620
tcgtggtatt gaccatgttg tgagaatgtt agggcaggac tatgaactat cagacctgat 70680
ggagattgca cagatgccag ttcgtcagat gccattcatc atgaaaactg atggacaaaa 70740
cctaaaacct gttgggacgc tacagaattt tatggcagag gtgaataatg cttaaacgca 70800
tttgggaagg tttggttgtt gatgcaccag cgattgtgat tggtatgttg attgttaacc 70860
tatttactga ctttgaacaa ggttcattgt ttggagccat gttactatgg gttatcttcg 70920
aaatattaga gatacaatta ggtatcactg aaaaactaag agaactcttt gcgaagtttt 70980
ctaaaaagat ttaaaatgaa agggtctctt cggagacctt tttagcatgt aaaggggcaa 71040
taatgaacaa agtacagatt attaaaaaga atggctcact cgaagaacct gatatcaaaa 71100
aagttttagc agcagtcaca aagtcagcta acagggttgg atataaagaa ctgccaccag 71160
atgttaccca agctcttgag tcagcattta tgaggattct ggtaaagtcc actaaacaga 71220
ataatttact aatttcggta aatgatattc acagtattgt tgagggtgct ttggcagaag 71280
tcaatcacga aatctatgag tcttactcaa catatagaaa ttaccgtaaa gaagttgctc 71340
aaaattggga tgagctttac cagaagacca aagatacact cttcttaggt gacagagaga 71400
atgctaactt tgacagtagt ttaatctcta caaaaggttc aattattcgt ggttatctga 71460
ctaaagaaat ctttaaacag taccatttaa caccggagga acttgaagcc attgaaaaag 71520
ggtttatcta tatccatgat ttaagagacc taatttttgg tggtatcaac tgttgcctgt 71580
tcgacattgg taaagtcctg aaaggtggtt ttgaaatgtc tggcatcgaa tattgtgaac 71640
caaagtctgt actgtcagcc ttacaggtta ttggtgatgt agttctttca gcaactgcac 71700
agcaatttgg tggctttact ttagcagaga ttgataaggt acttgtaccg tatgctaaga 71760
agtctctacg ttaccatgct gaaaaagcag catcttacgg tattcctaaa gaacattacc 71820
acaattatgt catggagcag ctacagattg aattaactca aggttttcag tctcttgaaa 71880
tgaagctcaa tactgtacct tgtagccgtg gggattttgc attcacaact ttgacattcg 71940
ggttacttga ctcagatatg tctaatgaag ataaccgact gcaatacatg attgcaagta 72000
ctatcctaga tgttcgtatg aatggacaag gtaagagcaa gaagcctgtt gtattcccta 72060
aactggttta tatttatgac cagaaaagac ataatgagaa tatctgtcaa ggccacctgt 72120
acagtaaagc tattgagtgt tgctctaaag cgatgtatcc agatttctta agtgtatctg 72180
gtcatggtgc tgtagcagaa gcttttgagc gttctggcaa ggtaatttcg ccgatgggtt 72240
gtagagcctt cttatctcca tatcttaatg aggatgggga agagttctac gttggtcgtg 72300
ctaacattgg tgctgtatct ttgaacttac caatgattta ccagtattct aaagagaatg 72360
gtttagattt ctggaaagag cttgataagt acctagaaat gattcgcagc ttccacaaga 72420
aacgttacga aatgattgct aatatgccag caagttctaa ccctcttgca tttacacaag 72480
gtggtttgta taaaggaact aagaagccta ctgacagggt tggttgggat attgtgaagt 72540
ccttcacagc ttcttttggg gttactgcgc ttgatgaact atctgttctt gctgaaggta 72600
aacgacttca tgaagttgga agctgtagtt ttgcatacga tgttctggaa tacatcaaca 72660
tgaaaactga agagtttaaa aatgaagatg gtttcttata tgcagtctat ggcactccag 72720
cagaatcact ttgtggaact cagctaaaac agttcagaga gatgtttggc gttagcaaag 72780
gtgtttctga taaggaatac tttacaaata gtttccatat gaatgttgca gcagacatca 72840
caccatttga gaagcaggac ttagaagaac ctttcttcca tatctgtaga ggtggaagaa 72900
tccagtatgt aagggtagct aatccagaga acttatcagc acttaagagt tgtattacaa 72960
gagggatgtt gaaaggtttc tatcagggac ttaactttga cttagcaatc tgtgaacatt 73020
gtggtaacag accaaaggct gatgttgaag aatgtgaggt ttgccattca catgatatct 73080
ctgtgattaa cagggtgtgc gggtatcttg gatggactaa gattaaaggt gaatcccgaa 73140
tgaacgatgc aaaaatcgct gagattcgtg acaggatttc tatgtaaaac ttgacaaggt 73200
gatgtgggtc ttgataggct tacatcacct tttttttttt attggatgtt agatatggca 73260
gatagtatta ttgggttatt cataggttct gtcttactag ggttcttttt aggactttct 73320
tattgtgaat tgagagataa gttaaaatgt ttaagatatc aaaaacgatg atatacatac 73380
tccacattct agtcttcttc tttggggtta gtgttatggt atggggtttt tcagacccac 73440
aatggagttt ctcatatcat ggtcagatgg atttatggtc atgcttcaaa ccatttttag 73500
ggttagctat agcattcagt gcattaccaa ctaaggtgaa attatgatta agttgagcca 73560
aaaacagtta gagtggatta aggattacgc ctcagagtgt ggctcttgtg aaaagaatca 73620
cgtaaggtac tcaacattcc acacggtctt cacattgtat gttggcgaca acgttctaag 73680
tgattccgta gtggatggtg taccgttacc taatgagtta cttgacaaat tagctgtagt 73740
tactggaacg tggagtgaag aagatggtca cgacctatct aacgatgttg tcttctacag 73800
tcttgaaaat attatgaatc cagagtacat tatgctaatg acttgtgcac aagactgtgt 73860
tccattacaa aacttcatta aagagcactg tgaagagttt attactaaac aggttccttg 73920
tcaggtagtg tttgaatgag taaaacaatt aaatagtatt ggtgaggtaa ctagatgaat 73980
tacatggaga ttcgaccatt tgacacagct aatggtgaag gggttcgggt aagcctcttc 74040
gtagctggct gtaaacatca ctgtgaaggc tgctttaaca gggagtcttg gaagtttaat 74100
gctggtaaag agtttactta tgcaaacctc tacggcatca ttaagttaat ggatgataag 74160
gccattagtg gactgtcaat acttggtggg gagcctctgg atgatagaaa cattcaagag 74220
gtcaccaaca tatgcaagcg tattaaaact gtttatccag aaaagtctat atggctttgg 74280
acaggttttc aactgcacga aaaaatccac ttagatgtga tgaaatatgt tgacgtggtg 74340
attgatggta agtatgattc atctaaacca acagttaaac catatcgtgg ttctgataac 74400
caaaacctct ggagaaaaga gtatgggtgg caaggtgatt gtcagtggag agccgagtaa 74460
gtctcgtcgt aaagtaggtt atcggtaaag gccactttac aatgtgctac agaacaaact 74520
taaccgttgg tgtcactgaa gaagaactta aagcattttg tgaaaagatt gagagagata 74580
cgaaatggga atctggaaca gttttaaatc aaaagtaaaa gcagcactgg catcactagg 74640
tatgctcact ggtgttgatg tgactcatct catgcagcac ttcaagatgg atgagaaact 74700
agctaatgag attatgaaaa gtatctatgt aggccgtggt aaaggtggta agaaacaagc 74760
acatcgacca actggtgcag cagctattaa acgggcagct aagaaagctc gtaaccgtaa 74820
acgtaataag aaggctaaat aatcatgagc aaagtttata acactcgtaa attacagatt 74880
ttcgtactgt gccagtttat ggctaaagag tataactgct actactgtgg cacagggttc 74940
atcagtgaca atgacggctc atatctaccg ttcaaagagg ctgtaaaact cttcaatgaa 75000
gaagtaagct ctaagaaaga tatcgaaaag gttaagctaa cctacagtaa gaaagataag 75060
aagattatct gcctagataa ctttgtaaaa gttactggag aagcaaaaga gtttatggat 75120
gagagtgaga tttcattcac aagcatcttg aaagtagctc agtaaacaga tactataagg 75180
gaactgcaaa ggttcccttt ttatttttgg agataacatg tatctgtcaa atctgaaacg 75240
ttcggctgca atgtcagttc taagactcag ctttgaggaa cgtcaagagt tcattgactc 75300
ccaccaatac gacccttcta actctaaccg catgattctc tggaatcgtg atagtactcg 75360
tgaaagggca ctattccgct attacccaca ttacaccata gataacctgt atgaatgttt 75420
tgttgtgaag aacactatca caattctcaa caaactttgc agatacacag gtaatcaatc 75480
cttcacatta ggtcaccaca agcctgttac aaaaggtggt gaacatcact gtgcaaactg 75540
gtttatccag actaaacttg ataaccagaa gcaaggcgat agtcttttaa gcacccctaa 75600
gatgacctat gaagagcaag agaaatatat caaaaataat atgccagatg tgcttgacaa 75660
cagctataca gatttggcaa tatctctcct gttgaagttc gagacagttt atagggcaac 75720
ttacgatggc tagaacagat tgggaagtaa aagaacttgt aagtgatgct ggaaatggct 75780
gtgaggtgta tatgattcgt ggccatgtgc cagacccatt agctattgag atgattgacc 75840
atcacacaag tggattctac aaagaactcg gtgaaccaag tgttgaaaga acttggacaa 75900
aaccagtacc agatagcaca ggtaactgtt cagtaatcta tcacattgtg aatccacaaa 75960
aatgcaaaac agctatggct gtgacattcg taacttttga ttgagagaga aaattatgaa 76020
aactattatt gaagccgttg tcaccgcaca agagaaattc ttccacacag aaaaatttaa 76080
tatcatccaa tttccatctg gagaaattgg tggaaacttc tctgaagact ttgttaagtt 76140
tactgagcgt aatgctggta agattgacaa tgttatattg actgtgcagg gatatgataa 76200
agatacactg tttgcattag ctttggctaa ggatgccgtt gacagtcttg ttccatacaa 76260
atcagcgatg aaaaccgttg ttttcgggtt cttaccaaat gctcgatatg accgccatat 76320
gttcaaaggt gattcagcag cactgaaagc ttttgccaac ctcgttaatg caatgggctt 76380
tgatgcagtg tgtgctcttg accctcacag taatgttgca gaaaatttat ttaagtgctt 76440
tcagagcatg aagcaaaaag atgtagcagc acactttgca agtgacccac gcattgattt 76500
cttggtagcc ccagatgcag gtgcagctaa gaagactgaa gagactgcta aagaggtaga 76560
taagccatat attacaatgt ctaaagtacg caaccttaag actggtgaaa ttactggtat 76620
gcgaatcctt gatactgttg atttgaccga taaaactgtc atgattcttg atgatatttg 76680
tgatggaggc cgaaccttca tagaagcagc taaacatctt cgtgaagcag gtgcaaaacg 76740
tgtggaactc tatgtaactc atggcatttt ctctaaaggt gttgaaaact tacttgacaa 76800
tggtgttgac catatctaca ctacaaactc tttaggggaa gctaaagacc gtggcttaac 76860
acattatacc caagttactg tagtaaccat tgattgaaac tttatagggg cttcaaagcc 76920
ccattttgag agagattaaa agatgactaa atcactttat gcagtaccag caggtttaaa 76980
cgcagatgct tacaaatctg gtcacattta ccagtaccct agtgcaacag aatacttgat 77040
gttcaatctg acaccacgta gtgataagtg gttcaacagc cccttagcaa ttgatggtgt 77100
agtcgctttt ggtattcaac gttttgttaa agattacctg atagaccact ggaacgccac 77160
cttcttcgaa cgtgacaaaa aagaagcaat tgacgaaatc ttagaagtta tgaacggtgt 77220
tctgggtaaa gatgctattg gtcgagaaca ttgggaagca ctccacgact taggttatct 77280
accagttgaa gtctacgctg tagaagaagg cacagttgtt cctatgcgtg taccaatgat 77340
tgtcttccag aacactgttt caggtttcca ttgggtagcg ggatatctgg aagatgcttt 77400
ctctgctgag atttggaagg cttgtaccat tgcaactatt gcattgcact acaaacgtat 77460
ctgtaagaag tggtctgacc ttacttgtga caacgactta catttgcctt atcagtgcca 77520
tgactttgct atgcgtggta tgtccggctt tactgatgac gcatttaacg ctgtaggtca 77580
cttaacaagc tttaaaggga ctgatagctt ccctgcggta tatacagcta aacgcatcta 77640
tggacagtcc tatccaatct ctgatattgg tagctctgta ccagccactg aacactctgt 77700
aatgtgtgca aacattgctt gggaaggtgg taatgagttg attgaagaag aaagacgctt 77760
caaaggtgag ttacaaacct tccgtcgctt cttaacagaa acttacccaa ctggtattgc 77820
aagtgttgtt tcagacactt ataatttctg gagaactgta tcagtaatac taccagaact 77880
ccgtaaagag attatggaac gtgatggcaa actggtaatt cgtcctgact ctggagaccc 77940
tgtacatatt gtcacaggat ataaagcaat tcacttagag tgtgctaaaa aggcttatta 78000
cgaacaccta agcaagcaga atgccagtga cacaatgttg agcaccgtcc tgaacatgaa 78060
gcttgaagat attaactatg ttatggctgg gtggttacgt gcagaaggct atgaaatggt 78120
ggttgacaga gaagactttg aagtagccga tacagtgttg ctgaaagatg cttacatcaa 78180
cggctctgca aacgttgtaa cacgtcctgt agcggagatt gatggagcta ttaagacact 78240
gtacaacatc tttgggggaa ctattaactc taaagggttt aaggtactgg atgagcacat 78300
cggccttatc tatggtgact ctatcacgct ggaacgtgca aacgaaatcc tgaagcgtct 78360
gtatgaaatg ggttttgcaa gctctaacgt agtgtttggt gtaggctctt acacttacca 78420
gtacatgact cgtgatacct ttgcatttgc tgtcaaagca actcttgcaa gcattggtgg 78480
taaagagatt atgcttgcaa aagaccctaa aacagatagt ggtgttaaga aatctgcttt 78540
tggtggtgta gcacccatgt gggatggcga aaagctgaaa gctgtagatg gctatgggtt 78600
ccagagcttt gcagatgtac ttgaccaccc agcttgtgct ttacgtctag tctttagtga 78660
ctctgagcag ttcggctaca caactcttgg tgatattcga agtaatattg acaagcagct 78720
ttaaaagtat atgataagag gctcctacgg gagccttttt aatttctgga gaatattatg 78780
aaaatcaaag agatgaacat caacattgtc ttagaagaac gttgggagaa catcaagaag 78840
cctgaagatg gtcataagtt ccttaacaaa atcttagtag cagctaaaga ggaactgact 78900
ggtaagattg cagcagcaat cacaataaag gtttgtgtaa aaggtcttcc agacaaccac 78960
caatttgcac ttgacgagtt taaagaaagc ttctacaatc caaacaaaca gatgcttgaa 79020
agtaactttg cagtgtctac aagtatcgtc catgacagaa gctttatcct ttacaagaat 79080
atgagaggtg agtcatgcaa gcatattgga tagaaatttt actgtcactt ggcagtgtag 79140
cagtctttgt ttaccttctt tgcaagtact atgcagagca caagaagtgt gattactgca 79200
atggggaagg gtatacaaga gcaggttgtt gccctatgtg tggcggttct ggtaaaatgt 79260
ttaataagta attcaacagt aaactaagag gaaagtatta tgcgcatggt aaacgaccac 79320
gcagaagtga ttaagagttc aacttctttg gagacatctc aagcacagat tacaatgaca 79380
cctgaaatgt tcagccttct gagttctggt gtatacacat ttaaagaaag ggcagtgatt 79440
cgtgaactgt catgtaacgc agtagatgct cagaaagaag ctggaagaga gaacatcccg 79500
ttccatgtgc atttacctac tcgttttgag ccttactttg aagttcgtga ttttggaact 79560
ggattgactc atgataaagt tatgagtttg tacctaaact acggggcttc tacgaagaat 79620
gactctaatg attacattgg tgcaatgggt atcggctcaa aatctccatt tgcaattgct 79680
cagtcattca cagtatctag ctatgttgac ggtgttgtta ataagtactc tgtttatctt 79740
gagaatggta tccctcaagt aactaagctg acaactaacc caacaaaaga acctaatggt 79800
ttagctgtac gtgtggcagt tgctgaccac cgtatctcaa agttctttga agaagctggt 79860
aatgtatact catactttgc tgtaaaacca gaaagtaata ttgtgtacga cgatgtatta 79920
gcagatatga acgtcattgc ccgtgaagag ggtgtttatg atgccatgat tcataagcaa 79980
agttggcgtt ctagtggtaa caggacagag tttaatgtgg tgatgggtaa cattgcctac 80040
cctgttaaca tggaagcatt acttggtgat gacttcttca aagtattgcc agaatttttc 80100
cgtaggagcg tagaccttgt aaacatctac atgcctattg gttcagttgc tattgcagct 80160
tctcgtgaag cattgcagat gaatgacacg acaaaaaatg ttatcattga ggctactaaa 80220
aagataactg aagccattac aaaggatgtt atcaagaaag ttaatagcca acctacactc 80280
atggatgctg cacaggctta tgctgagtta cgtttgaact cacgagagat gtttaatgct 80340
gtgtgtccaa agctagagtg gggaggcgtt aagcttgatt ctctggaaga agaattgcta 80400
aacattcgtc gtgggattat ctacgcagaa gacggctcag tcatttatga acgtgatggt 80460
aaaggtaata ttaaggttga cagtaaagga aacaacattc ctaaagtgga ctacctatac 80520
aatccagttg cttatgtcaa gttcaactct ttagagagta agattcgtgc aacagcactt 80580
tcctacactc aagaggcaag tatgttcaat atctttggtg caatgcgtaa aagccaaatt 80640
gaacagtttt tgttcgtgat taatgaccgt cgcaataaaa acggtactga gaagactgta 80700
ggacgcaatc aaattttgcg tggtgcatgt cgagactacg ccagtgagtc aaatctgttt 80760
cacagataca atggtattgt ctttgtattc tcgactgaaa aagagttaga tgatttaatc 80820
aatctacaca aacttgataa aagcttgtta aagattgtga agatgtctga taaggaacac 80880
cactatcagc gtaaagaagc tgtaaggggt gttgtaaaac tctggaaagc tgttccagca 80940
gaaactaaag cttcatacaa agaggtgtca gaagacttag acacaattga agagcctcag 81000
ctttatatta aggcagtagg tgacacagtt gatggtagat gtttttgctc atctccagag 81060
gatgtagcta agtcagttgc taatgttaca ggcaagacag tctatgtttt ccgaaaagca 81120
aattggaaaa agataccaga agactggatt gaagtagatg agaagctctt gaacgacagc 81180
ttaactgatg ttcattggat taaccataac aggtatatga cacgcatcta catggatggt 81240
gtcctcgacc ttacaagcag ttggattatt gccagaaact ttacgttcaa taacaggaag 81300
atttctcgcg ggtattgtta ttcaagggat actattaaga caatcttctt agaagggaat 81360
gaagatgctg ttgaggcaat gttcggtaag attcagtatg tggctgcacc gtttgcatac 81420
acttacacta tcagtgttct tcaggatttg aagtcatgcc ttgataatga cacaaaactg 81480
tacaaaaaga ttaaaaaagc aggtgaccgc atggttatca agctgacaaa ctatctttca 81540
aaaagaaaac aagaaaactt cttgctttca cacttagatt ggaataaagt atcacctatc 81600
gaagtgagta agtttttagg ctttaatgtg aaatgtgttc cagaaggtac aacaatttac 81660
gattaaagtg tttgacaagg ggctttgaag ccccttagaa tagcctcaca aattgattga 81720
ttaactaaca agagagagta ataagatgac tactaaaact aaagcacaga ttgacgcaga 81780
aatttacaaa ctggttaaag aaggtaagct gactaaaaca gctattgcac agaagttcaa 81840
tacttcaacc cgttcagttg gtcgtgctgt agagcgtcat gaagcaacct taaaaggcaa 81900
gaagaaggca acttctacac cagctacgaa gactttgaaa caagttgcta agacttttaa 81960
aaagaaagct ggcaaaccag ttgaaaaggt cgttaaagac actgtacaga aagctccagt 82020
aaataaactg cacgaagcta tgcagaaaga tgacaagatt gagtacatga ttactggtga 82080
ttctgtaatt atgacttatg gttcagaatc tgaaattgtt gagtctactc atccgaacta 82140
tcaagagatt gtagttcatg ttgtgaaagg tgagttcaag aaagcatttg aactgatgaa 82200
cattcgtaag tctatcgaaa acttcactca gggtgctatc acaatcaaag gcgacaagct 82260
attctacggt gctgttgaga tgcgttctac tctggttgac cgtattcttc acatgatgaa 82320
aactggcgat aaaggttttg aacgacttgt aatgttcttc gaaaaactga tggaaaaccc 82380
atctaaagat tctgtagaac aactttgggg atttgtatct caccttgatg ttgaaattga 82440
tgaagaaggc tacatcattg gttggaagaa agtctctact cgtgaaggca agctggttga 82500
ctctcacacc tacaaagtgc ctaatgattt gggtaacatt gtagaaatgc cacgttggat 82560
ggttgataat aaccgtaacg taacttgctc tcaaggtctt catgttggtg cttgggatta 82620
tgttcgttgc ttctcaggtg atacaatcct gaaggttcgc gtacatccac gagatgttgt 82680
atctgttcca actgattaca atgatatgaa gatgcgttcc tctcgttatg aagttgcagc 82740
aatcgttgat aatcaacgta aagtgctgga agcatgggac ggtaagactg aagctttgca 82800
tgtcatcgtt ggcactgctg gtgaactcat ctctcaacgt aaacgtgaaa tctaataagt 82860
aatttcttaa aaggctgctt cggcggcctt ttttatttgt attttgtgca gagtgctgta 82920
taattgatgt cacgataaac taaacaggag aaccaatgaa gaaattgatt ctaggtttgt 82980
gcttaatgtt tacagcacac ttatcttatg cagttgactg cccagagcta tcaattagtc 83040
aaaaagtaaa catgttaaaa gcttaccagt atggtgagaa taatatgggg aaaggctggg 83100
gtattactct agcagctata gccttacaag agtcagagtt aggtctgaag gtagagaata 83160
aaaagaccca cgactatggt attttccaga atcacttgaa gactgttgta aagcgtaaca 83220
aaattagtcc taatgtggct aaaaagaaac tcttaaaaga ctttgactat gcagcaagag 83280
agacacataa agagcttgag ttttggacta aggtacatgg tcacccaaag tcaaagaaga 83340
ctttacaaaa agttttagca tcatataatg ctgggtattc gtacaaaatc cctaaagcta 83400
agaaatactc tcaagatgtc tataacaata tgaaagttat cgctcaatgt gaatttgcaa 83460
caaacatttc taaggtaaat catgaaaaaa ttaagaaagt ctgatgaagt cctgtgccat 83520
gcttacgact tgcaccctca tgagttaggt cttgattcct gtgtatggac tccagaacag 83580
tgcagggatt ttgaagatac tgcaagagaa gttgtatgtt cacttgaaga gttccacaca 83640
tcagaaccaa ttgtgagcgt tgtggacaaa gagactggac aaactgtagg tgtaaggcgt 83700
gacagcttag tcatagtcaa taaagacctt gtagaaaaag gtaaccttat cttagcagat 83760
attgatggtg ttcttacaaa ttttaatcac gaagattgct caacggaatt gactgatggg 83820
tcattctcac agtacactaa tcttcttgac tctgtaagag caaaaccaac atatgttttt 83880
agtattattg atgcaattgc taatcatgct gccattggac tcttgacagc gagaggtgaa 83940
tctcagagaa tacctactga gatgttttta aggcataaca tagagcatga ttacatgctt 84000
tttatgcgtg gttttggaac taactctata agtgcagaaa gtttaaaagt gaggatgatt 84060
cagtcttgca ttcttcctta ttttaatata gtatgcttta tagaagatac agagaagaat 84120
gttcagaagg taaacagaat ccttccacac attaaaacca tgttagttaa acattgagag 84180
aaaaacttat gaacaatatc attacggtag cactggacga tacggcaaac aaatctgaag 84240
tagttcgtaa aatgattcaa ggtaactttg aaggtaaaat tttccgtgct gttaatgtaa 84300
aagctgatgg cagtattcgt gaataccgtg cattgttgaa tgttaaaaag cacgtaaaag 84360
gtgctggttc aacgactgca cacaaagaaa acctgatgac tatctatgat atgggtaagg 84420
cttcagagtt aggtgctgaa ggtatcgtta aagaaggtgc tccgtatcgc tctttcaatc 84480
tggaaactac tcttatgctt tcctttacga gtggttctaa aacaatcact tatctcttta 84540
ctgatgctgc aacggtatct gctattaagg atagcactgt caaagctggc gtagttgctg 84600
ctgcaaaagc ttcttcgatg gctgcgaata tccttgctaa agtcctcggt taaggttaag 84660
atacaggctc cttcgggagc ctttttcatt tcaggagatt gttaaatgac atttaaagag 84720
ttctgtcaag ctactttcat aattgttttc ttagttggtg caggtgtctg gggaggatac 84780
tcttacagag actatcaagt tgctgaaact gagctaaata atgagaaact aataagtgtt 84840
gctaaagatg cttatcagga aggtttagcc acactcagca ccaattacaa aaatgatttg 84900
aaagatgtgc ttgctaagaa taagcataca aaagaggtac taacatatga aaaaactaag 84960
ccagagtttt ataatgtttg tgctactgat aactatgtca gggtgttcaa cgaacaaagt 85020
gaacagtaca ttcaaaaact cccaagtaag tgagagtgat aagtacactc aagaagaatc 85080
acggtatatc atcaaaggca acacaggcag tgatgtagca gcagcccttg agttctatcg 85140
tgacggcttt taccagtgca caatcaaagc taacaacctt attgacatga ttttattagg 85200
gaataagcag caatgacaga gaatcaagat actttctatg tagaaggtta cttactgttg 85260
ccacgaccaa aagaaactta tatgcgaatt gatttcttac caaccattat ggataatgtg 85320
atgtgccata tctttatgca aggtgtcaca gcacagctta agcatgttgg taaagagtgc 85380
aaaataaagg ttgacactca tccagaaatc aaagagaatc actacacatg gttcttacca 85440
gactctaaag aaatcttagc agttcttaaa acgaggaagt aactatgcag attaatggaa 85500
gagactttgt agccgtttac tacgagaaag gtaaagaagt tggcgtagca caagtgtctt 85560
atggcaatgg taagtggcta tatggaacaa tcgcagtagt tggaacaaga agtgatacaa 85620
aaacttttaa agattgtgtt gaccttcttg aagaatccat tcagaatcat tggtgtctga 85680
tatggatgac tgacaatgaa gtgatagaac gcttcaaaaa gattgatatc aacattgata 85740
gtattgagca tgttgattta tatgagctaa ctgagaaggt aaactatgag agtaattcta 85800
gccagagata aaaagacacg gaaactcatc cggtcagcag tattcattgg aagaaatgaa 85860
gtcatcccgt ttacagcaaa cgatgtgata gcctacagga aaaacttagt atttagtgat 85920
tgcttgcgtg gcttcatctc ccaaccgttg gaagacttta aaagccgttg tggtaaaggt 85980
atcatcattc aagaggcaat tatcaatggc tgacttctgc aaagactgtt ctatagaaat 86040
gtttggacgt gatacaggtg acttaaaagg tcttatcact gaagatgact ttaaagctgg 86100
ctacgcaatg ccagtaatct gtgaaggttg tgggtgcatc tgggtagacc acgaaggaca 86160
gcgtgtaaaa ccttcagaag ataaagaatc ttgggagaga tgttaaatgg gtattgtaaa 86220
aatcatcaaa ggtgatatct ttgatgcctt tgataaaggc aaatttgata tcattggtca 86280
tggttgcaac tgtatgaatt tcatgggtgc aggtattgca gcaactattt ctaagcgtta 86340
tccaaaagtg tatgatactg acacagaagt ttacctgttt gcaggtggca ttcgtcacaa 86400
accctgtgaa aatttactag gtaatttctc tgtagcacgt ttaaaacaag gccgtatagc 86460
taacctttac acccaacttc atactggtaa agatgcccga tacagtgctt tagagtcatc 86520
tttgaaacag cttaacagat actgtgaagt taaccagttg aagaaagttg gtctacctat 86580
gattggtgca ggtatcggtg ggcttgaccc tcaagctgtc acagttatca ttaatcaggt 86640
gatgaagagt gtagatgttt atctgtatgt ctatgaagca aaaatgtata atgagttaca 86700
ctcaggttgg aaaaactact gtgaaccaga atactttgca ggtgtagttg tgttcacaga 86760
taacgaagtt accctcttca gacgaagaaa aggtaagata catcaaagta accctccggt 86820
tgaaaagatg tctctgagta acgctctagt tacccacctg tcgaagagca atcacaggat 86880
tgcagtaaca tttggcagtg atgcagatac ttatatctat gcaagaactg atgaggatat 86940
cgagacagtt ttctcttcgc cagaagttac cttcttagac gcaaagaact aagaaacttc 87000
gtaggatatt cacaatagac taagcctttc ttatctggta aatttttcag gtaaggaggg 87060
ctttttcatt tcagaattgt gcaggtatat ttctatagag aaatctgtag aggaaatttt 87120
gaactccaaa gagaaaattc attttcaaac tgtgcaggta atctccaaag ggttttttca 87180
ttttcaaaac gtgcagagaa aaatcaggtc gggcaagaaa aatcatttta gatttgtgca 87240
ggtaggtttt tatagagagc cagaccaacc cctctccccc tcttactccc cctctcccca 87300
aaagtcaaga gaaaaatttg tagcggattg aaaaataatt cttgatttgt gttctgtttt 87360
gtggtagtcg tgtgcgcccg tttcattaca gttttgccaa aaatattttt aaaatttttt 87420
cttgactctt tgaacgtggt tatctagtat ttgcatcaac ggggagggaa tgaccttccc 87480
cactaaataa agtcctaaac tggagattca aaaatggcat atcgtgcacc taaattcatc 87540
aacaaagaca actttcgcaa cgcactggag aagtcacttg atgaaaattt taaaggcaac 87600
attatcgttg ttcacgcatt caattttaag tatgatgtaa acgggaatag aatcaaccat 87660
ta 87662
<210> 2
<211> 87662
<212> DNA
<213> 人工
<220>
<223> 在分离自环境的噬菌体中发现
<400> 2
taatggttga ttctattccc gtttacatca tacttaaaat tgaatgcgtg aacaacgata 60
atgttgcctt taaaattttc atcaagtgac ttctccagtg cgttgcgaaa gttgtctttg 120
ttgatgaatt taggtgcacg atatgccatt tttgaatctc cagtttagga ctttatttag 180
tggggaaggt cattccctcc ccgttgatgc aaatactaga taaccacgtt caaagagtca 240
agaaaaaatt ttaaaaatat ttttggcaaa actgtaatga aacgggcgca cacgactacc 300
acaaaacaga acacaaatca agaattattt ttcaatccgc tacaaatttt tctcttgact 360
tttggggaga gggggagtaa gagggggaga ggggttggtc tggctctcta taaaaaccta 420
cctgcacaaa tctaaaatga tttttcttgc ccgacctgat ttttctctgc acgttttgaa 480
aatgaaaaaa ccctttggag attacctgca cagtttgaaa atgaattttc tctttggagt 540
tcaaaatttc ctctacagat ttctctatag aaatatacct gcacaattct gaaatgaaaa 600
agccctcctt acctgaaaaa tttaccagat aagaaaggct tagtctattg tgaatatcct 660
acgaagtttc ttagttcttt gcgtctaaga aggtaacttc tggcgaagag aaaactgtct 720
cgatatcctc atcagttctt gcatagatat aagtatctgc atcactgcca aatgttactg 780
caatcctgtg attgctcttc gacaggtggg taactagagc gttactcaga gacatctttt 840
caaccggagg gttactttga tgtatcttac cttttcttcg tctgaagagg gtaacttcgt 900
tatctgtgaa cacaactaca cctgcaaagt attctggttc acagtagttt ttccaacctg 960
agtgtaactc attatacatt tttgcttcat agacatacag ataaacatct acactcttca 1020
tcacctgatt aatgataact gtgacagctt gagggtcaag cccaccgata cctgcaccaa 1080
tcataggtag accaactttc ttcaactggt taacttcaca gtatctgtta agctgtttca 1140
aagatgactc taaagcactg tatcgggcat ctttaccagt atgaagttgg gtgtaaaggt 1200
tagctatacg gccttgtttt aaacgtgcta cagagaaatt acctagtaaa ttttcacagg 1260
gtttgtgacg aatgccacct gcaaacaggt aaacttctgt gtcagtatca tacacttttg 1320
gataacgctt agaaatagtt gctgcaatac ctgcacccat gaaattcata cagttgcaac 1380
catgaccaat gatatcaaat ttgcctttat caaaggcatc aaagatatca cctttgatga 1440
tttttacaat acccatttaa catctctccc aagattcttt atcttctgaa ggttttacac 1500
gctgtccttc gtggtctacc cagatgcacc cacaaccttc acagattact ggcattgcgt 1560
agccagcttt aaagtcatct tcagtgataa gaccttttaa gtcacctgta tcacgtccaa 1620
acatttctat agaacagtct ttgcagaagt cagccattga taattgcctc ttgaatgatg 1680
atacctttac cacaacggct tttaaagtct tccaacggtt gggagatgaa gccacgcaag 1740
caatcactaa atactaagtt tttcctgtag gctatcacat cgtttgctgt aaacgggatg 1800
acttcatttc ttccaatgaa tactgctgac cggatgagtt tccgtgtctt tttatctctg 1860
gctagaatta ctctcatagt ttaccttctc agttagctca tataaatcaa catgctcaat 1920
actatcaatg ttgatatcaa tctttttgaa gcgttctatc acttcattgt cagtcatcca 1980
tatcagacac caatgattct gaatggattc ttcaagaagg tcaacacaat ctttaaaagt 2040
ttttgtatca cttcttgttc caactactgc gattgttcca tatagccact taccattgcc 2100
ataagacact tgtgctacgc caacttcttt acctttctcg tagtaaacgg ctacaaagtc 2160
tcttccatta atctgcatag ttacttcctc gttttaagaa ctgctaagat ttctttagag 2220
tctggtaaga accatgtgta gtgattctct ttgatttctg gatgagtgtc aacctttatt 2280
ttgcactctt taccaacatg cttaagctgt gctgtgacac cttgcataaa gatatggcac 2340
atcacattat ccataatggt tggtaagaaa tcaattcgca tataagtttc ttttggtcgt 2400
ggcaacagta agtaaccttc tacatagaaa gtatcttgat tctctgtcat tgctgcttat 2460
tccctaataa aatcatgtca ataaggttgt tagctttgat tgtgcactgg taaaagccgt 2520
cacgatagaa ctcaagggct gctgctacat cactgcctgt gttgcctttg atgatatacc 2580
gtgattcttc ttgagtgtac ttatcactct cacttacttg ggagtttttg aatgtactgt 2640
tcactttgtt cgttgaacac cctgacatag ttatcagtag cacaaacatt ataaaactct 2700
ggcttagttt tttcatatgt tagtacctct tttgtatgct tattcttagc aagcacatct 2760
ttcaaatcat ttttgtaatt ggtgctgagt gtggctaaac cttcctgata agcatcttta 2820
gcaacactta ttagtttctc attatttagc tcagtttcag caacttgata gtctctgtaa 2880
gagtatcctc cccagacacc tgcaccaact aagaaaacaa ttatgaaagt agcttgacag 2940
aactctttaa atgtcattta acaatctcct gaaatgaaaa aggctcccga aggagcctgt 3000
atcttaacct taaccgagga ctttagcaag gatattcgca gccatcgaag aagcttttgc 3060
agcagcaact acgccagctt tgacagtgct atccttaata gcagataccg ttgcagcatc 3120
agtaaagaga taagtgattg ttttagaacc actcgtaaag gaaagcataa gagtagtttc 3180
cagattgaaa gagcgatacg gagcaccttc tttaacgata ccttcagcac ctaactctga 3240
agccttaccc atatcataga tagtcatcag gttttctttg tgtgcagtcg ttgaaccagc 3300
accttttacg tgctttttaa cattcaacaa tgcacggtat tcacgaatac tgccatcagc 3360
ttttacatta acagcacgga aaattttacc ttcaaagtta ccttgaatca ttttacgaac 3420
tacttcagat ttgtttgccg tatcgtccag tgctaccgta atgatattgt tcataagttt 3480
ttctctcaat gtttaactaa catggtttta atgtgtggaa ggattctgtt taccttctga 3540
acattcttct ctgtatcttc tataaagcat actatattaa aataaggaag aatgcaagac 3600
tgaatcatcc tcacttttaa actttctgca cttatagagt tagttccaaa accacgcata 3660
aaaagcatgt aatcatgctc tatgttatgc cttaaaaaca tctcagtagg tattctctga 3720
gattcacctc tcgctgtcaa gagtccaatg gcagcatgat tagcaattgc atcaataata 3780
ctaaaaacat atgttggttt tgctcttaca gagtcaagaa gattagtgta ctgtgagaat 3840
gacccatcag tcaattccgt tgagcaatct tcgtgattaa aatttgtaag aacaccatca 3900
atatctgcta agataaggtt acctttttct acaaggtctt tattgactat gactaagctg 3960
tcacgcctta cacctacagt ttgtccagtc tctttgtcca caacgctcac aattggttct 4020
gatgtgtgga actcttcaag tgaacataca acttctcttg cagtatcttc aaaatccctg 4080
cactgttctg gagtccatac acaggaatca agacctaact catgagggtg caagtcgtaa 4140
gcatggcaca ggacttcatc agactttctt aattttttca tgatttacct tagaaatgtt 4200
tgttgcaaat tcacattgag cgataacttt catattgtta tagacatctt gagagtattt 4260
cttagcttta gggattttgt acgaataccc agcattatat gatgctaaaa ctttttgtaa 4320
agtcttcttt gactttgggt gaccatgtac cttagtccaa aactcaagct ctttatgtgt 4380
ctctcttgct gcatagtcaa agtcttttaa gagtttcttt ttagccacat taggactaat 4440
tttgttacgc tttacaacag tcttcaagtg attctggaaa ataccatagt cgtgggtctt 4500
tttattctct accttcagac ctaactctga ctcttgtaag gctatagctg ctagagtaat 4560
accccagcct ttccccatat tattctcacc atactggtaa gcttttaaca tgtttacttt 4620
ttgactaatt gatagctctg ggcagtcaac tgcataagat aagtgtgctg taaacattaa 4680
gcacaaacct agaatcaatt tcttcattgg ttctcctgtt tagtttatcg tgacatcaat 4740
tatacagcac tctgcacaaa atacaaataa aaaaggccgc cgaagcagcc ttttaagaaa 4800
ttacttatta gatttcacgt ttacgttgag agatgagttc accagcagtg ccaacgatga 4860
catgcaaagc ttcagtctta ccgtcccatg cttccagcac tttacgttga ttatcaacga 4920
ttgctgcaac ttcataacga gaggaacgca tcttcatatc attgtaatca gttggaacag 4980
atacaacatc tcgtggatgt acgcgaacct tcaggattgt atcacctgag aagcaacgaa 5040
cataatccca agcaccaaca tgaagacctt gagagcaagt tacgttacgg ttattatcaa 5100
ccatccaacg tggcatttct acaatgttac ccaaatcatt aggcactttg taggtgtgag 5160
agtcaaccag cttgccttca cgagtagaga ctttcttcca accaatgatg tagccttctt 5220
catcaatttc aacatcaagg tgagatacaa atccccaaag ttgttctaca gaatctttag 5280
atgggttttc catcagtttt tcgaagaaca ttacaagtcg ttcaaaacct ttatcgccag 5340
ttttcatcat gtgaagaata cggtcaacca gagtagaacg catctcaaca gcaccgtaga 5400
atagcttgtc gcctttgatt gtgatagcac cctgagtgaa gttttcgata gacttacgaa 5460
tgttcatcag ttcaaatgct ttcttgaact cacctttcac aacatgaact acaatctctt 5520
gatagttcgg atgagtagac tcaacaattt cagattctga accataagtc ataattacag 5580
aatcaccagt aatcatgtac tcaatcttgt catctttctg catagcttcg tgcagtttat 5640
ttactggagc tttctgtaca gtgtctttaa cgaccttttc aactggtttg ccagctttct 5700
ttttaaaagt cttagcaact tgtttcaaag tcttcgtagc tggtgtagaa gttgccttct 5760
tcttgccttt taaggttgct tcatgacgct ctacagcacg accaactgaa cgggttgaag 5820
tattgaactt ctgtgcaata gctgttttag tcagcttacc ttctttaacc agtttgtaaa 5880
tttctgcgtc aatctgtgct ttagttttag tagtcatctt attactctct cttgttagtt 5940
aatcaatcaa tttgtgaggc tattctaagg ggcttcaaag ccccttgtca aacactttaa 6000
tcgtaaattg ttgtaccttc tggaacacat ttcacattaa agcctaaaaa cttactcact 6060
tcgataggtg atactttatt ccaatctaag tgtgaaagca agaagttttc ttgttttctt 6120
tttgaaagat agtttgtcag cttgataacc atgcggtcac ctgctttttt aatctttttg 6180
tacagttttg tgtcattatc aaggcatgac ttcaaatcct gaagaacact gatagtgtaa 6240
gtgtatgcaa acggtgcagc cacatactga atcttaccga acattgcctc aacagcatct 6300
tcattccctt ctaagaagat tgtcttaata gtatcccttg aataacaata cccgcgagaa 6360
atcttcctgt tattgaacgt aaagtttctg gcaataatcc aactgcttgt aaggtcgagg 6420
acaccatcca tgtagatgcg tgtcatatac ctgttatggt taatccaatg aacatcagtt 6480
aagctgtcgt tcaagagctt ctcatctact tcaatccagt cttctggtat ctttttccaa 6540
tttgcttttc ggaaaacata gactgtcttg cctgtaacat tagcaactga cttagctaca 6600
tcctctggag atgagcaaaa acatctacca tcaactgtgt cacctactgc cttaatataa 6660
agctgaggct cttcaattgt gtctaagtct tctgacacct ctttgtatga agctttagtt 6720
tctgctggaa cagctttcca gagttttaca acacccctta cagcttcttt acgctgatag 6780
tggtgttcct tatcagacat cttcacaatc tttaacaagc ttttatcaag tttgtgtaga 6840
ttgattaaat catctaactc tttttcagtc gagaatacaa agacaatacc attgtatctg 6900
tgaaacagat ttgactcact ggcgtagtct cgacatgcac cacgcaaaat ttgattgcgt 6960
cctacagtct tctcagtacc gtttttattg cgacggtcat taatcacgaa caaaaactgt 7020
tcaatttggc ttttacgcat tgcaccaaag atattgaaca tacttgcctc ttgagtgtag 7080
gaaagtgctg ttgcacgaat cttactctct aaagagttga acttgacata agcaactgga 7140
ttgtataggt agtccacttt aggaatgttg tttcctttac tgtcaacctt aatattacct 7200
ttaccatcac gttcataaat gactgagccg tcttctgcgt agataatccc acgacgaatg 7260
tttagcaatt cttcttccag agaatcaagc ttaacgcctc cccactctag ctttggacac 7320
acagcattaa acatctctcg tgagttcaaa cgtaactcag cataagcctg tgcagcatcc 7380
atgagtgtag gttggctatt aactttcttg ataacatcct ttgtaatggc ttcagttatc 7440
tttttagtag cctcaatgat aacatttttt gtcgtgtcat tcatctgcaa tgcttcacga 7500
gaagctgcaa tagcaactga accaataggc atgtagatgt ttacaaggtc tacgctccta 7560
cggaaaaatt ctggcaatac tttgaagaag tcatcaccaa gtaatgcttc catgttaaca 7620
gggtaggcaa tgttacccat caccacatta aactctgtcc tgttaccact agaacgccaa 7680
ctttgcttat gaatcatggc atcataaaca ccctcttcac gggcaatgac gttcatatct 7740
gctaatacat cgtcgtacac aatattactt tctggtttta cagcaaagta tgagtataca 7800
ttaccagctt cttcaaagaa ctttgagata cggtggtcag caactgccac acgtacagct 7860
aaaccattag gttcttttgt tgggttagtt gtcagcttag ttacttgagg gataccattc 7920
tcaagataaa cagagtactt attaacaaca ccgtcaacat agctagatac tgtgaatgac 7980
tgagcaattg caaatggaga ttttgagccg atacccattg caccaatgta atcattagag 8040
tcattcttcg tagaagcccc gtagtttagg tacaaactca taactttatc atgagtcaat 8100
ccagttccaa aatcacgaac ttcaaagtaa ggctcaaaac gagtaggtaa atgcacatgg 8160
aacgggatgt tctctcttcc agcttctttc tgagcatcta ctgcgttaca tgacagttca 8220
cgaatcactg ccctttcttt aaatgtgtat acaccagaac tcagaaggct gaacatttca 8280
ggtgtcattg taatctgtgc ttgagatgtc tccaaagaag ttgaactctt aatcacttct 8340
gcgtggtcgt ttaccatgcg cataatactt tcctcttagt ttactgttga attacttatt 8400
aaacatttta ccagaaccgc cacacatagg gcaacaacct gctcttgtat acccttcccc 8460
attgcagtaa tcacacttct tgtgctctgc atagtacttg caaagaaggt aaacaaagac 8520
tgctacactg ccaagtgaca gtaaaatttc tatccaatat gcttgcatga ctcacctctc 8580
atattcttgt aaaggataaa gcttctgtca tggacgatac ttgtagacac tgcaaagtta 8640
ctttcaagca tctgtttgtt tggattgtag aagctttctt taaactcgtc aagtgcaaat 8700
tggtggttgt ctggaagacc ttttacacaa acctttattg tgattgctgc tgcaatctta 8760
ccagtcagtt cctctttagc tgctactaag attttgttaa ggaacttatg accatcttca 8820
ggcttcttga tgttctccca acgttcttct aagacaatgt tgatgttcat ctctttgatt 8880
ttcataatat tctccagaaa ttaaaaaggc tcccgtagga gcctcttatc atatactttt 8940
aaagctgctt gtcaatatta cttcgaatat caccaagagt tgtgtagccg aactgctcag 9000
agtcactaaa gactagacgt aaagcacaag ctgggtggtc aagtacatct gcaaagctct 9060
ggaacccata gccatctaca gctttcagct tttcgccatc ccacatgggt gctacaccac 9120
caaaagcaga tttcttaaca ccactatctg ttttagggtc ttttgcaagc ataatctctt 9180
taccaccaat gcttgcaaga gttgctttga cagcaaatgc aaaggtatca cgagtcatgt 9240
actggtaagt gtaagagcct acaccaaaca ctacgttaga gcttgcaaaa cccatttcat 9300
acagacgctt caggatttcg tttgcacgtt ccagcgtgat agagtcacca tagataaggc 9360
cgatgtgctc atccagtacc ttaaaccctt tagagttaat agttccccca aagatgttgt 9420
acagtgtctt aatagctcca tcaatctccg ctacaggacg tgttacaacg tttgcagagc 9480
cgttgatgta agcatctttc agcaacactg tatcggctac ttcaaagtct tctctgtcaa 9540
ccaccatttc atagccttct gcacgtaacc acccagccat aacatagtta atatcttcaa 9600
gcttcatgtt caggacggtg ctcaacattg tgtcactggc attctgcttg cttaggtgtt 9660
cgtaataagc ctttttagca cactctaagt gaattgcttt atatcctgtg acaatatgta 9720
cagggtctcc agagtcagga cgaattacca gtttgccatc acgttccata atctctttac 9780
ggagttctgg tagtattact gatacagttc tccagaaatt ataagtgtct gaaacaacac 9840
ttgcaatacc agttgggtaa gtttctgtta agaagcgacg gaaggtttgt aactcacctt 9900
tgaagcgtct ttcttcttca atcaactcat taccaccttc ccaagcaatg tttgcacaca 9960
ttacagagtg ttcagtggct ggtacagagc taccaatatc agagattgga taggactgtc 10020
catagatgcg tttagctgta tataccgcag ggaagctatc agtcccttta aagcttgtta 10080
agtgacctac agcgttaaat gcgtcatcag taaagccgga cataccacgc atagcaaagt 10140
catggcactg ataaggcaaa tgtaagtcgt tgtcacaagt aaggtcagac cacttcttac 10200
agatacgttt gtagtgcaat gcaatagttg caatggtaca agccttccaa atctcagcag 10260
agaaagcatc ttccagatat cccgctaccc aatggaaacc tgaaacagtg ttctggaaga 10320
caatcattgg tacacgcata ggaacaactg tgccttcttc tacagcgtag acttcaactg 10380
gtagataacc taagtcgtgg agtgcttccc aatgttctcg accaatagca tctttaccca 10440
gaacaccgtt cataacttct aagatttcgt caattgcttc ttttttgtca cgttcgaaga 10500
aggtggcgtt ccagtggtct atcaggtaat ctttaacaaa acgttgaata ccaaaagcga 10560
ctacaccatc aattgctaag gggctgttga accacttatc actacgtggt gtcagattga 10620
acatcaagta ttctgttgca ctagggtact ggtaaatgtg accagatttg taagcatctg 10680
cgtttaaacc tgctggtact gcataaagtg atttagtcat cttttaatct ctctcaaaat 10740
ggggctttga agcccctata aagtttcaat caatggttac tacagtaact tgggtataat 10800
gtgttaagcc acggtcttta gcttccccta aagagtttgt agtgtagata tggtcaacac 10860
cattgtcaag taagttttca acacctttag agaaaatgcc atgagttaca tagagttcca 10920
cacgttttgc acctgcttca cgaagatgtt tagctgcttc tatgaaggtt cggcctccat 10980
cacaaatatc atcaagaatc atgacagttt tatcggtcaa atcaacagta tcaaggattc 11040
gcataccagt aatttcacca gtcttaaggt tgcgtacttt agacattgta atatatggct 11100
tatctacctc tttagcagtc tcttcagtct tcttagctgc acctgcatct ggggctacca 11160
agaaatcaat gcgtgggtca cttgcaaagt gtgctgctac atctttttgc ttcatgctct 11220
gaaagcactt aaataaattt tctgcaacat tactgtgagg gtcaagagca cacactgcat 11280
caaagcccat tgcattaacg aggttggcaa aagctttcag tgctgctgaa tcacctttga 11340
acatatggcg gtcatatcga gcatttggta agaacccgaa aacaacggtt ttcatcgctg 11400
atttgtatgg aacaagactg tcaacggcat ccttagccaa agctaatgca aacagtgtat 11460
ctttatcata tccctgcaca gtcaatataa cattgtcaat cttaccagca ttacgctcag 11520
taaacttaac aaagtcttca gagaagtttc caccaatttc tccagatgga aattggatga 11580
tattaaattt ttctgtgtgg aagaatttct cttgtgcggt gacaacggct tcaataatag 11640
ttttcataat tttctctctc aatcaaaagt tacgaatgtc acagccatag ctgttttgca 11700
tttttgtgga ttcacaatgt gatagattac tgaacagtta cctgtgctat ctggtactgg 11760
ttttgtccaa gttctttcaa cacttggttc accgagttct ttgtagaatc cacttgtgtg 11820
atggtcaatc atctcaatag ctaatgggtc tggcacatgg ccacgaatca tatacacctc 11880
acagccattt ccagcatcac ttacaagttc ttttacttcc caatctgttc tagccatcgt 11940
aagttgccct ataaactgtc tcgaacttca acaggagaga tattgccaaa tctgtatagc 12000
tgttgtcaag cacatctggc atattatttt tgatatattt ctcttgctct tcataggtca 12060
tcttaggggt gcttaaaaga ctatcgcctt gcttctggtt atcaagttta gtctggataa 12120
accagtttgc acagtgatgt tcaccacctt ttgtaacagg cttgtggtga cctaatgtga 12180
aggattgatt acctgtgtat ctgcaaagtt tgttgagaat tgtgatagtg ttcttcacaa 12240
caaaacattc atacaggtta tctatggtgt aatgtgggta atagcggaat agtgcccttt 12300
cacgagtact atcacgattc cagagaatca tgcggttaga gttagaaggg tcgtattggt 12360
gggagtcaat gaactcttga cgttcctcaa agctgagtct tagaactgac attgcagccg 12420
aacgtttcag atttgacaga tacatgttat ctccaaaaat aaaaagggaa cctttgcagt 12480
tcccttatag tatctgttta ctgagctact ttcaagatgc ttgtgaatga aatctcactc 12540
tcatccataa actcttttgc ttctccagta acttttacaa agttatctag gcagataatc 12600
ttcttatctt tcttactgta ggttagctta accttttcga tatctttctt agagcttact 12660
tcttcattga agagttttac agcctctttg aacggtagat atgagccgtc attgtcactg 12720
atgaaccctg tgccacagta gtagcagtta tactctttag ccataaactg gcacagtacg 12780
aaaatctgta atttacgagt gttataaact ttgctcatga ttatttagcc ttcttattac 12840
gtttacggtt acgagctttc ttagctgccc gtttaatagc tgctgcacca gttggtcgat 12900
gtgcttgttt cttaccacct ttaccacggc ctacatagat acttttcata atctcattag 12960
ctagtttctc atccatcttg aagtgctgca tgagatgagt cacatcaaca ccagtgagca 13020
tacctagtga tgccagtgct gcttttactt ttgatttaaa actgttccag attcccattt 13080
cgtatctctc tcaatctttt cacaaaatgc tttaagttct tcttcagtga caccaacggt 13140
taagtttgtt ctgtagcaca ttgtaaagtg gcctttaccg ataacctact ttacgacgag 13200
acttactcgg ctctccactg acaatcacct tgccacccat actcttttct ccagaggttt 13260
tggttatcag aaccacgata tggtttaact gttggtttag atgaatcata cttaccatca 13320
atcaccacgt caacatattt catcacatct aagtggattt tttcgtgcag ttgaaaacct 13380
gtccaaagcc atatagactt ttctggataa acagttttaa tacgcttgca tatgttggtg 13440
acctcttgaa tgtttctatc atccagaggc tccccaccaa gtattgacag tccactaatg 13500
gccttatcat ccattaactt aatgatgccg tagaggtttg cataagtaaa ctctttacca 13560
gcattaaact tccaagactc cctgttaaag cagccttcac agtgatgttt acagccagct 13620
acgaagaggc ttacccgaac cccttcacca ttagctgtgt caaatggtcg aatctccatg 13680
taattcatct agttacctca ccaatactat ttaattgttt tactcattca aacactacct 13740
gacaaggaac ctgtttagta ataaactctt cacagtgctc tttaatgaag ttttgtaatg 13800
gaacacagtc ttgtgcacaa gtcattagca taatgtactc tggattcata atattttcaa 13860
gactgtagaa gacaacatcg ttagataggt cgtgaccatc ttcttcactc cacgttccag 13920
taactacagc taatttgtca agtaactcat taggtaacgg tacaccatcc actacggaat 13980
cacttagaac gttgtcgcca acatacaatg tgaagaccgt gtggaatgtt gagtacctta 14040
cgtgattctt ttcacaagag ccacactctg aggcgtaatc cttaatccac tctaactgtt 14100
tttggctcaa cttaatcata atttcacctt agttggtaat gcactgaatg ctatagctaa 14160
ccctaaaaat ggtttgaagc atgaccataa atccatctga ccatgatatg agaaactcca 14220
ttgtgggtct gaaaaacccc ataccataac actaacccca aagaagaaga ctagaatgtg 14280
gagtatgtat atcatcgttt ttgatatctt aaacatttta acttatctct caattcacaa 14340
taagaaagtc ctaaaaagaa ccctagtaag acagaaccta tgaataaccc aataatacta 14400
tctgccatat ctaacatcca ataaaaaaaa aaaggtgatg taagcctatc aagacccaca 14460
tcaccttgtc aagttttaca tagaaatcct gtcacgaatc tcagcgattt ttgcatcgtt 14520
cattcgggat tcacctttaa tcttagtcca tccaagatac ccgcacaccc tgttaatcac 14580
agagatatca tgtgaatggc aaacctcaca ttcttcaaca tcagcctttg gtctgttacc 14640
acaatgttca cagattgcta agtcaaagtt aagtccctga tagaaacctt tcaacatccc 14700
tcttgtaata caactcttaa gtgctgataa gttctctgga ttagctaccc ttacatactg 14760
gattcttcca cctctacaga tatggaagaa aggttcttct aagtcctgct tctcaaatgg 14820
tgtgatgtct gctgcaacat tcatatggaa actatttgta aagtattcct tatcagaaac 14880
acctttgcta acgccaaaca tctctctgaa ctgttttagc tgagttccac aaagtgattc 14940
tgctggagtg ccatagactg catataagaa accatcttca tttttaaact cttcagtttt 15000
catgttgatg tattccagaa catcgtatgc aaaactacag cttccaactt catgaagtcg 15060
tttaccttca gcaagaacag atagttcatc aagcgcagta accccaaaag aagctgtgaa 15120
ggacttcaca atatcccaac caaccctgtc agtaggcttc ttagttcctt tatacaaacc 15180
accttgtgta aatgcaagag ggttagaact tgctggcata ttagcaatca tttcgtaacg 15240
tttcttgtgg aagctgcgaa tcatttctag gtacttatca agctctttcc agaaatctaa 15300
accattctct ttagaatact ggtaaatcat tggtaagttc aaagatacag caccaatgtt 15360
agcacgacca acgtagaact cttccccatc ctcattaaga tatggagata agaaggctct 15420
acaacccatc ggcgaaatta ccttgccaga acgctcaaaa gcttctgcta cagcaccatg 15480
accagataca cttaagaaat ctggatacat cgctttagag caacactcaa tagctttact 15540
gtacaggtgg ccttgacaga tattctcatt atgtcttttc tggtcataaa tataaaccag 15600
tttagggaat acaacaggct tcttgctctt accttgtcca ttcatacgaa catctaggat 15660
agtacttgca atcatgtatt gcagtcggtt atcttcatta gacatatctg agtcaagtaa 15720
cccgaatgtc aaagttgtga atgcaaaatc cccacggcta caaggtacag tattgagctt 15780
catttcaaga gactgaaaac cttgagttaa ttcaatctgt agctgctcca tgacataatt 15840
gtggtaatgt tctttaggaa taccgtaaga tgctgctttt tcagcatggt aacgtagaga 15900
cttcttagca tacggtacaa gtaccttatc aatctctgct aaagtaaagc caccaaattg 15960
ctgtgcagtt gctgaaagaa ctacatcacc aataacctgt aaggctgaca gtacagactt 16020
tggttcacaa tattcgatgc cagacatttc aaaaccacct ttcaggactt taccaatgtc 16080
gaacaggcaa cagttgatac caccaaaaat taggtctctt aaatcatgga tatagataaa 16140
ccctttttca atggcttcaa gttcctccgg tgttaaatgg tactgtttaa agatttcttt 16200
agtcagataa ccacgaataa ttgaaccttt tgtagagatt aaactactgt caaagttagc 16260
attctctctg tcacctaaga agagtgtatc tttggtcttc tggtaaagct catcccaatt 16320
ttgagcaact tctttacggt aatttctata tgttgagtaa gactcataga tttcgtgatt 16380
gacttctgcc aaagcaccct caacaatact gtgaatatca tttaccgaaa ttagtaaatt 16440
attctgttta gtggacttta ccagaatcct cataaatgct gactcaagag cttgggtaac 16500
atctggtggc agttctttat atccaaccct gttagctgac tttgtgactg ctgctaaaac 16560
ttttttgata tcaggttctt cgagtgagcc attcttttta ataatctgta ctttgttcat 16620
tattgcccct ttacatgcta aaaaggtctc cgaagagacc ctttcatttt aaatcttttt 16680
agaaaacttc gcaaagagtt ctcttagttt ttcagtgata cctaattgta tctctaatat 16740
ttcgaagata acccatagta acatggctcc aaacaatgaa ccttgttcaa agtcagtaaa 16800
taggttaaca atcaacatac caatcacaat cgctggtgca tcaacaacca aaccttccca 16860
aatgcgttta agcattattc acctctgcca taaaattctg tagcgtccca acaggtttta 16920
ggttttgtcc atcagttttc atgatgaatg gcatctgacg aactggcatc tgtgcaatct 16980
ccatcaggtc tgatagttca tagtcctgcc ctaacattct cacaacatgg tcaataccac 17040
gagcttttgc aaaattcttt gcagtctcac actgagggca tccagttttg gagtaaatta 17100
cataagtcat taaggaacct ctaaaaatcc actattcaaa tcatctacaa cagtattcaa 17160
taggtacgca ccgttctgtt gctcctgatt agcattctgc tctttatcaa tttccatctt 17220
cttaatcata tacttcaaag gtggttcttt aggagctaca aaatctctgg gaatgccaaa 17280
catatcatac agtggggcag cattatagta aacccactca tgaagaagct ttgtatttaa 17340
gccaactaca gcacgtcctt cggagaagat ataatacgac catttctctt cactttcaac 17400
tacttcatct aagattactt taatctctgg aagaatttgt tgaaaagctt tctgccactc 17460
atcatctctt aaagtttctt ttaaaacttc aatatcaatt ttagtgtgaa ggatttcgtc 17520
aagcataatt ttctggacag cttgagcaat accctgaaat ttatcttgag catcaagtgc 17580
aaaagtacat gcaaaggatg ccataaaaga tataccttca agcgcagtca ctgcaaacaa 17640
cccttttaga atcactttat ggaagtgtaa agggtctttg tccagaagtg agtcacgcac 17700
ataactcaga cgatagttta taccttcatc cagtaattct tcaagaacac gattcacagt 17760
ttttaatcgg tcttgtacag caacattctg gttaatctca tctaagattg tttcagggtt 17820
tttaatacat tgtcttacaa tctctgagta agtaagagca tgtaggtttt caatctcaga 17880
ctgcttcata attgcagttg catagatgtc atcagagata aagggtgcaa aggcaaatgc 17940
caagctctta gcaacttgag tgtctgcttc ccactgccac ttaagaatct caagcattac 18000
acctgacatt gatgctggga cactctcaaa atcaagacgt gattgttcaa aagggaactc 18060
atcttctgac cagtcttgtg ctttttgttg tttataaagt tcaaagattt ttgggtagtg 18120
cttattgagt gagtcaaaag tttttctctc accacctaaa aagattgggt gctggttaat 18180
catagcgtga tttctccttt ctttatcatt tctaaggctt gttttctgtc taaaacttcc 18240
caattctctt tgttaaacat ctcataaggt cttgcgtaga ttttaccatc tgctgtagag 18300
atgtaagagg ctccagtgac ccataagtca tcattctgct ttatcatgat acctgtgctg 18360
ctcacatagt acaatgtttt gcggggtttg tagagcaagt atattggtcg ctcatgtttt 18420
tctattaaat ctttcatggt ctctccaaag ttatttttag agggtcttct acgaccctca 18480
tagttattaa aactaaacac cacaaccctc gcaataagca tcttgcagtg cagatttacc 18540
tacaccaatg cgactgttaa ggtagtacat ggttttcata cctaccgagt tggcataaat 18600
catgtacttc aaagcttgag ccaatgatac cttcttagct tttgcgtagt caacatagaa 18660
atctgaagag atagcttgac cagtgaactt ttgaacaatt gcataacaat caatcaggtc 18720
aaaggtgtca ataccccaag caatttcata tacatacttc aactcttcat aatctggaac 18780
aataaacagc acgttaccag ttgcagactt tttagttaaa ataaagtcac gaattgggta 18840
caagccattt gtcgtattag ttgccagtga agaactctca ttaggcatgt aagcttctaa 18900
tacagagttt cggattccac cattttcttt gatacgttgt gctaagtcat cccaatcata 18960
tcgtagtttt gcatcatgct tttcatcaat cttcttgtta gctgtctttg gaggaaccca 19020
gccttcagga tacttagtga acttcatgta ctctggaacg ccacgctctt tagcaagtct 19080
cagagaggct tcatgcagat agtaagagtg catctctgcc agttcatgaa gtttcgtttt 19140
acctgctcgt gaagagtagt tcacgtagtt cttcgcaagg taatgagcca catttgtaag 19200
gccaatccca acagaacgac gcttctgaac atggttgcgc atcgatggat acggataatc 19260
cataaggtca ataacggagt caaccattgc aagagcataa taagcaacgt cagcgtattc 19320
atcttctgaa attctccctg caaccaaact agctaggaag caaagagcca cctcaccatc 19380
ctctttcaca gcatcgtctc tgtaaaggtc tgtctctttc tcaaaaccat acactggcaa 19440
cacaatttcc atacaaagat ttgacatctt caatggttct ttaaatggtg tatgtgtgtt 19500
tgcgttgtta gtgaagaatg gatacacacg acctgtagca taacgctgtt gaataaacag 19560
cttagcaatt tcacgagcct tgactcgtct gtgcttaact cctgaatgta ctgcgtgacc 19620
aactgccata gcaaactcat cagcagatgc tgtgtagaac atgtcataga gttttggtgc 19680
atctttgtaa gagaatagca accaatctgt atcatactga acacactgcc agaaataatc 19740
gtttgtgccg aatgagtagt ccatctcatt aatacgttta gaaggaaccg ttgtagggtg 19800
cttcaaacgc agtaaatctt caatctgcgg gtctagagca gtgtagaagt tattagctga 19860
accaccacga ctcttctgtt tgtttgcctc tacagatgaa cgtacaagct tgtaataagg 19920
cagtttaccc atgtgttcga tagtgttttg acggatacca tcaccaatag tgcgagtctc 19980
catcagcata ccgataccag cttgctttgt agtcatatca taagcaacct ttgcagcaat 20040
accaagtgat tcagcagtat cgtttgcctt aatcaagcag catgacgcat aacctgattt 20100
agttgccctc aaaccgttta aataaggcgt aggagcgtta atcttcaggt cagataggta 20160
agtgtacagc ttgataacat cttgcagtct acggtgcttt ggttgcttct caaaggcttt 20220
catagccata cccataaaca taaattgtgg tgactcaaaa agtcttcccg ttttaatatc 20280
acggatacca tacttgtctc tgaactgttt caagactgca taaccgtaag agatatcttt 20340
tgagtgcacg atgtaacctt gcaggtattc aagctcttcc tgtgagtaat ccatcttctc 20400
ccaaagtcct gctctctcca tatttttaac gaaggtaacc agcgtaggaa ccttagtaaa 20460
gcctccaaag gcttctttgt agataattcc tagaagtagc cgtccagcca tgtctgagta 20520
ctcttgagtt tgtttatcaa cacaaacatc aatcatagct tggtgcatct cttttgtagt 20580
gcaacctcca tagacacgtt tcatggcttc catagtgact tctgaccaga tgattccacg 20640
cttatctgcc catgatgccc acttattcag tctttctggg tcaaagctta ctactgtacc 20700
gtttgatttt ttaattgtct taatcatttt tgaatcctac agatgaaaag agcctccgaa 20760
gaggctccct agtttaaatc ttgaaaattt gctcattctg ccacatatcg taaaacttat 20820
cacttacctt tgtgactgaa gaggtgtgca tacaattcac gaaccatgat atatcactat 20880
tcataattat ttcaagctgt tttgtgacat ccacattcat gtcttcaaca acgtaattac 20940
ctcgcatctt gcacacaact cttccaaaca agattgcact tctgcctctt ctgtcgtcac 21000
agtacatgac tgtgtccccg tgcttaacat cttgtccgat actatcaaca cctaatttag 21060
cacctgagat aatgtcatca taagatagct tttttgcttt agacacggta tttctccaag 21120
tttcttttta gatataacca accatagtca gttctttcta cacccatgaa gagtggagcc 21180
attacaactt taccaaaagt attcctgtgt tgacagattt caaaagaata ctctttctca 21240
atatcaatcc cgtatttgta cagaactgct tttaaaactt gttcattatc cagaagtgta 21300
tctggagtct caccatactt ctcaagtaac gggtcattca taaacaccgt catagagata 21360
ttgtagtttg agaagtggct cttctgagcc acaccttctt tattaatgtt ctgtgaatcc 21420
attataacca acccctttta accagcactt caggttatta gcatcataat tcagttttgg 21480
gtatgaggtt acatggaact gctcaccatc atagtagaca atatctgcaa tccatagtcc 21540
attctcttta cagaaatctt tatctgcttt agaggtaatt ggattaactt ctacaaaacc 21600
atctacaccc aaatccttta agctacatac aatacctttg cagataacgc atgtctcaga 21660
tactaccaca aaaagttctt ggctttcaag ttctacaatg tattcaccaa gctgaacttt 21720
agggagatta gattgcttca taatcttccc atcggctgca cgaacaattg cataccattc 21780
tttgccatca actactgaca ttctgatgat acactcttga ccagtacgct tctcaatatc 21840
tgcaagacgt tttaaagctt cttcatagtt gtctgtgtat tttaggtcat tgttatggca 21900
aacagcttcc atagcaccat tatggtcatg ttgtgacagg taaatcaaac cgtcaagaac 21960
atattgcaaa tctgcctgag catccagtgt ctcaatgggg tctttctctt caattgcttt 22020
tacaagctct tttgcttctt caagcataca tagagattga gatttcaaag attcccaata 22080
ctcattgctg tagggttgct tttgagtgtt tccacaacga agattccagt tttttactga 22140
ttctcttgag ttaaacattc tgactcctta aaatatctgt taacctttag ctctcgtgtt 22200
tcaatatatt ccagatgctc acgtagctct tgacgtttaa gcattaggtg ctgcatttga 22260
gattcaacag cttcaacctc tttaattatt gactctctgg catttgcaag aatgcgctca 22320
agttcttcaa tcttgctatc aacttttgct acatgactca cagctttact tttgttaaac 22380
attttattct ctctcaatag ttatcaagca cagtttgctt tcattacctt atggattctt 22440
ttttcagcat cttcataagt ttctttagta ataaacttaa ttgctgcaat attggcattg 22500
tagaagagtc tgagcttaga gtcaatcctt tttgtcatta catcgaattt atgttgaagg 22560
tttgcttcac cataaactag accacctttt gtgtggtagg tttgaataat ataaaagtca 22620
aaaaattctt ttccaaagct ctcaatatct tttttaatat actcagaaga agtctcataa 22680
gtcatccaat cactttcctt agtgactacc ttcttccgag tcttgccagc aacttttctt 22740
ttggtcacac tattaagctg tttctttccg atataatatt gtccggtttt cttacagtat 22800
actaaataga caaagccaaa atgtttagtg gggtcaactt ccccacacaa agatacccaa 22860
tgaccatagg tggggcaatt gccaaatcct ttaatcttca ttcatacacg gctcccaatt 22920
atatttctta aaagagaaat tatcttttgg atttctctct tgatatgcta cccagaaatg 22980
ttgttccatt aactctaatg gtgttttggt aacagtctgt ccatcccatg acacataggt 23040
gtaagactct tttttagcat agagttcata gatagcatca agacactctt tataggtctc 23100
tttaccatct aaagcattca tcacagctac tttgccagca cctttaaggc cgaagtagtt 23160
gtctgcatta tctccagcaa cagcctgata acacaaaaat ttaaaaccta caccaactgt 23220
ttttgcagct tttggttttg atttgatagg acaatcccag atatcaccaa cattattatc 23280
agcaataaaa attagtggtg acttttcata agtcatatca atacaataag tcccttcagc 23340
ttgtcgaagg tctttgtcaa tactcataag agcagccttc ttacccatct tttcagcttt 23400
agcaattacg atggaatcgg cttcgaagcc tccctttaag agcttgaact ctggtcttga 23460
cagaagatat tcacgacaag caactaagtg tgttggtgtg acagcatctt tacggttacc 23520
ttgatattgg tgctcaagac ctttaatgtc tttatgttta tgtacaccct tctctgttaa 23580
gtagcctacc caagttcttt ctttaccaac aaccttaagc cattcctgaa gaacctgctg 23640
agtagccatg atagcttctt tttcactctt agcttctttc caagtctgtc tttcccattc 23700
atcttcatca aatgtcaggc caagctcttc cacaaggatt ctctggtctg ctaaccatct 23760
tgcagcatct tttgcattat caaatggttc agattcttct gctgtgagtt tattgacata 23820
tttatatttt gctttctcaa ctacacaagc acctttatag gcaatgctat cagagtcaat 23880
aaagacatgt gtaactgaat cgggaagttt tgttagtgta tacttctcca ttgtgactct 23940
ccatatgaaa aagccccata ctaagtacag ggctttaaag taacttttag agattagtct 24000
tctgtatcga agtcttcatc ttcttcgtca tctgggtctg gcaagtcttc atcgtcacca 24060
tcatcagaat tgttcgaagg tttgtggtct gttgcgtctt cttcagtgat ttcaccatta 24120
tcttcaacac catcaagacc aagcatagcc agttcgtctt catccagttc aggttcaccg 24180
ttagcaccgt taccaccagt gtaaggtaca agagtatcaa tgataaactg ttcctgaata 24240
ggttttgtca gaacattgtt ctcaaaagtg tagaagtgag tagaaagaat cacactacca 24300
aaagaaccgt taccaactgc aatatctgga tgaattacat catagttctt gtcatcttca 24360
tgcttatcgg atgcttgagc tttgattttc ttcatcggct gtttaacagc tacacgctta 24420
ccatttactt cttcaatcag cattacaggg aatgactgtt tagctgtcca tacagcacca 24480
tctttataag ctgctgcacg acttactttc aagatgtagt aagtgtctgc ttcaaaaggt 24540
ggtttacaac caaacttctc ttcgaagtca tctgcatcaa ctgcttcagt agtaacttta 24600
tcccaacctt ctgggttttt cttagactta gtaaactctt taaacagctt gttaccatct 24660
tctgccagaa ttgaaacact gtagttgcag tctttgcctg ggaatttctt atcaatggat 24720
ttaccttttc ccggacgtgg tgaagtgttc aggtaataaa accaaacatc tttcagaagg 24780
taacgcagag tttgacgttc agtaccattg tacttctcta ccggagcttt cattttaaca 24840
actttagaca ttatttaaac ctctatctca atttatgaag agtaccaatt ctaatttgtt 24900
gatactctat tgtcaaacat taattactgg aagttgtgct tatctttaga agctgcttta 24960
cgtgcagttt ttccagttct tacacgcttc tcttcatagt attttgctgg tttaccagca 25020
gtggctttta aggaatcccc aaaaacttgt tcaaatgctt tagagtgttt catgatattc 25080
tccgatatac ttgatattca aacacatttt ctttaattat aaacagccaa ccaatattat 25140
gtcgaggtat agtaatagtg actgttatca tactgtgatg gaacttttct tctacctcaa 25200
cgcctccatc acatttcatg tagagcattc tatagaacct atcaaccttt gtcaaacact 25260
cttcctgaaa ttcatttaaa cttttttctt caaagaaggg tatgaacccc tctcttttgt 25320
aactatcact cagtttcaaa cgggcagtca tctgcatcct cacttaatac aggtggaatc 25380
gttgagcttt gacgctgttc ttcagtatag acttcaccag tctcacggtc aaacacttca 25440
tcctcataat gaggtaaaga gtcgtcataa tggtcttcag cttcaccaat accaaactgt 25500
tgacgaatat tttctgctgc accatcaatg tcaacaccac atccagaagc tttgataaga 25560
cgtcctgtgt ctggattgta ccaagtatga ccagcaatac ctgttgactt accatgacga 25620
cgacacttag ttaacttgat ttttgtcaag tttttcttaa cagggtctgg gtcaaccttg 25680
ttacgcatta acagaatgtt gttcatggaa atctggaaat acgcaccaga acccttaata 25740
tcctcttcag agatatctcc accttcagag ttagctttct gaccacctgc actcttacga 25800
acgtgacaaa cgtttacctg tgcatactgg tagcgtttgc aacgacgcaa tagttcagac 25860
aagacttcct cttcatccgt atcagaacgt gacagagcca atgtaatagg gtcaagaata 25920
ataatcttac agtctaaact gttaacaaga tagtcaacaa actccagcag gttatcttgg 25980
tcaatcgccc cttgatggtc aacgatatgg atgcgacgac cttttgaaag ctctgcgtgt 26040
gctcccttta attcatccca atcccgttca tcataaggaa tctcagaaat ctgtttgctc 26100
aggtggattg cacagagcat ttccatcaac tcttcatagg tatcttctac aggaattaca 26160
ccgatattat aatcagtttc tttccaagct gaataaatca tctcacgagt gtaagctgac 26220
ttacctactg aagatggtgc tgcaatagtc gtaatctcac ctaaaccgta accaccgtaa 26280
gttagcctgt tcaagtctcc gaaagattct ggaaaaggaa tcaatggaat ctgaccacga 26340
ttcttcattg cctcaaaacc gtctgcaaag ttcttgatac cagcagggca gtaacgaggt 26400
gcattgtaga tacgctgctt aaatccttcc agaactgtat ctttctcttt atagaacttt 26460
gtccaccatt cattaaggtc ttttacacct tctggatact ggaataaacg aaccttctca 26520
ataggtagga taccagcagc ctctttagtg gctttagcac ctgcttcatc gttatcaaag 26580
cacaagtaaa tttcatcaaa tgatgtaatg tactgatagt tatctttgat agacttgatg 26640
tttgcacctg atggaacaga tacgtgacag taattcttac gacgagactt gtccttaatc 26700
gcaagagaag tcatatagat tgctgtcgca cattccatct caccttccca gatgaatagg 26760
cggttaccac cttctggagc aatccatgaa ccgaacattg ccagttcacc tttaatatct 26820
ccaacaccac cagaaaagtc ttttaactta ccgcgtaggt gctcttttgg atggtcttct 26880
gggtaacggt gacgaacacg gtagccaaca tgctctagct tgccatcttc attgcgtttg 26940
taagttgggt agaaatgcgc atcaatttca ccgtcactat caatgtcaac cttgataccc 27000
aaacgctcaa gaacttttgc aggaatcttc ctgtccttta agtccattgc ttcaaggttg 27060
tcttttacat cgtctaaatc catcccacgg aaagtacggt ttttattgtc tgaaccagta 27120
gaataagtgc tcacgatttg tcctttatca aaatcccact ctgggaaacc tttgttacaa 27180
ctaaagcaag tcatcgaata agaatcatcg tcatgatgat agattgaacc agccactgat 27240
gaaccacaac gtggacatgc acaatgacca acaaactgac cagcctcttt caatttacga 27300
cctttagaca ttagcacctc ttcgttgtag ttctgccttc aatccatttt caatcttgtc 27360
cagttcatga atttcatctg caatctcttt ccttcgtgat tcaactctct taagacgttc 27420
aatcattacc tcattggaaa gagatgagag ttccactaaa cggtggtcaa taactttaaa 27480
attgtctttt actctcatct tcattctctc ttttaaactt taatgatggc tcttagcttg 27540
ttctcaaggt ctgtaagagt accattatta tgaataatgt cacgctcata tttggtggat 27600
agcccatttt ctgaaacgtg tgatgaaatc ttatccacat tgtctctttt tacttcaata 27660
gtttggtgtg caaatctact cagccactca gcttcagagt caaatcttaa atcactgatt 27720
aagacaaaac catattgatt tctaagtgag caactttcat agaatctaac cattcttttt 27780
tcaaggtctt tagaccagaa cttgtctccc attactttac ggacaacttc agtgccccaa 27840
atctgttgaa tttgtcttga tgagaattta tactttttgc taaaccccag acgtgtcaat 27900
agagttggtt tagcaacctt cttaagctcc atgattaatc gtccggttaa ctctgacata 27960
agcttgaagt ccatatggta acgttcatct ctgaaggtga actccatagc ttccgtaact 28020
ttaaacatca gttcaggata tgacaagtca aatacctgtg gagtctcttt agtttcacca 28080
tacaggtcat tccaagtcag gtcaaatatc tttgatgcag acagtttaag gttgtctgca 28140
aaggccatta cagcaacatt gtagccgtgc tcatcctcta agatgttctt cacaatagaa 28200
catgaggtgt cttttccaga acgtgccttt ccagtaaatg cgataatatt actcatttta 28260
tctccattaa ttactaaaaa gcccccaact aaggaggctt gtaagattta taaagactta 28320
gtgcagttgc tcagcatact ctttttcagc catagctgag atttgcttca caaaatcatc 28380
accgaagtta atgcgtagtt tctcttcaat gattgaagca ccaatgttca ccaaaacatc 28440
attgattgct tgcatactaa cgccaccagt catctcttca atgaactcaa tacttacacc 28500
catcatctgt gaaagttgga ttagtgcaac gatattcatc atcgtagaga tagctggaat 28560
catcagtgca actttcacct gaagaggttc tatatcagct acagagtcat tttcaacagc 28620
ttcttccaaa gactctttag tttgctggat aatttctttg attcgtgggt ttaattcttc 28680
tacaccccaa tcaagttttt catatcgtga aagtttttct tccatgcgct tctcaagagc 28740
agccataaca gcactagttg atgaaattaa caggtcaatt agttcatcgt tacctgcgag 28800
tagtagcaag tctttttctt cagtggtcat ggcaatataa tcatttttct gcatctcata 28860
aaaaatgaac tctgcaaggg catcaacacc tacaacgata gatgctaaag caagtgtttt 28920
gtcgcaaagc atcatgttaa cctgttctgc aaaagcaccg ttatcagact tgtcttcagg 28980
caagttatag cttgcaggca taaactgttc tgtgtagttg ttaccacgaa gaatcatagc 29040
catgctttct acggcattga ttaaaaatgc ttcgtcaata cctttctctt caagcatctt 29100
ttctacatcg atattcatat tatttctctc tcagttggtt aagtttcttt ctagaacatt 29160
ttaagttttc ggatgaagaa aagtcaagtg tcttttcaga acgtggataa tagtgcctat 29220
cccaagaagt ttcaacatcg ttaatcagtg acgctagatt acacaggtct gtaccacttt 29280
gcaacctctt tttaagagaa tctagtaaag caactgtgtc tttagcttta cgtctcgctg 29340
tagcaacatc tttcatatgc tcaaatacag agcattttaa gtcatcttca tagttttcag 29400
acaactctat ttcatgttgt atatcaacca ttctcctgtg atggtgagca tattcccgtt 29460
gagcagcaat gtcaagttct tccaactttt gcaaagctct aacaaagtca gctatgtctg 29520
ggtgaacaaa cattttctgc atgtaagcct cttttaagta actattaagt tttaatcttt 29580
tatcttaacg tattctatac gttatacttt aaagcttttt aaaagctatt aaataatctt 29640
ttaagacttt taaagtatct gtatagttaa actgttaagt aaccttttaa gtaaaaaccc 29700
acttcgcaaa gattaacacc ttgtcaagtg cattgtcaac ttgcttttta taattttttg 29760
cagtaatgta tgaggttaat gattttagag ggagtaaaaa tggaaaacgt agattttaaa 29820
aacttacatt tggttggtga tacagaaact gatggtttac tccttgagtt cactaaagtt 29880
cacgtaatgg cttttgcaga ctataaatct gatgatgaag agcctcctgt atgggtcttt 29940
acagatgaac ctatcctcgg tcacaagtat accaagtaca ttaagggtgg cttacgtgaa 30000
ggtattgagt ttgcgttaaa ggcaaaacgt ctttgcattc ataatggtct aggttatgac 30060
tggtgggttt tcaatcacat tgcaccggat ttatggaact ttgataatcc aaagtgtaag 30120
ccgtggagta atttctttca ggattctctt atccagtctc gtgttcagtg gatggataga 30180
ccaactccaa agggttataa aggtgctcat ggcttggctg catggggtgc tcgtgttggt 30240
gttcgtaaac cagagattga acattggggt gtgtggaatg cagaaatctt cactcgtgtt 30300
gtagaagata tccgtattaa cgccaaaact aaacgggcac ttgataatga gtatctcaag 30360
cttaagaagt gtggcataga cacttatgaa acctacatgc gagctaaaga aacatctttc 30420
tggatgagtc aacaagctat caatggttgg aaagctgata aagagcttat ggagttccat 30480
gtaaaggaac ttgacaagtt gactaatgag cttgcttcag aggttgaacc gcatctacct 30540
ccaactatta agaccaaagg taaagtcact ggagaagagt ttgcaaaagc ttggaatgag 30600
tatgttgaaa catttggtca tgcagatgga ctgaagagaa ttaccaagta ccctaagaca 30660
aagtaccgtc agcaggtacg taacggtgag atgcagacat atgaaatcaa gccatttggt 30720
aagccaacta caaagatttt taacattgaa aagagaaatt gctatacacc aaccaactct 30780
gtaactggtg aagagtacaa ggaaggtttt gtagcaatga aggatgctcg tgcaatttgc 30840
aacgagttga atgcaaagat tggtaagaaa tgcaaagact ggaagccagt aaaaacagtc 30900
aaaactgtga agtactacaa cagtcatgtt gttaaccact ttgaacttga gtcaagtcgc 30960
tatacaggtt tgattgatgc accatataca ccaattgagt ttgaagtttc tcgtatgact 31020
caggtagcag ttgttaaaga ctacttgaaa tcagttggtt ggattccaga tgactggaac 31080
tacaagaaag actctgatgg tcgccctgtc aaagtttgtc gtttcaaaga caataaaaag 31140
atgattacaa agcatcctaa gtggcaggaa atggttgagc ggtgtggttt gagttatgtt 31200
gaacacgaag gcgtccagta cattgagcat aactggtctg tgaagaagta cacagatttg 31260
cttgagcctt gcttaatccg tacttcacca aaacttactg aatcatctta cgatacgatt 31320
gaaggtgagc ttggacagaa gattgctaaa tactacactt tgatgcaccg acgcagaact 31380
attgagaact caaaggatga tgaaaaaggt tggttgaacc agattcgtcc tgatggtcgc 31440
cttagtgctg gtgcaatggt gtttggtact tcaactggac gtatgacaca gtatggtatt 31500
gtaaacgtac cgtctggtgc tgctgtctat ggggaaccaa tgagggcagt gtggatttgt 31560
gaggaaggta ctaacgttgt ctctgtagac atgaactcag cccagctagt tcttctttgt 31620
aactttatgg gtgataaaga cttcaccaaa gcggtaacgc aaggtaaaga agaaattgag 31680
ttcattcgtc aagaagatgg acgctattac tgtaaacact ttgatgagta cctcaaccca 31740
gagattgata agtaccttcg ctatgactct gagaatgacc tgtacgttgt ctattcaggg 31800
actgatgcac atacactgaa cagtatttac tttaacttga acgatgagca ggacatcttg 31860
acttgtcgag ctactcagga tgagaatctt cttcatgaga ttagcaaggg tcgtaagaaa 31920
gctaagaatg gtatctatgc actgctgttc ggtgcaggtg atgagaagtt tgctaagacg 31980
attaaagctg caactactca ggaaggtgcg ctgactaaac agacttactt tattcgtttg 32040
cctaaaatta aaaagctgct agatgacttg gaagctgact acaaagcaac taaaaaggca 32100
cttgaagagg tgtttggtaa gactgctgca atctctaaag ggggttttgt aaaggttgct 32160
ggagcttggt tgtggtgtaa atctccgcat aagctgttga attatttgct catgggttca 32220
gaggctcaga ttcaaaatga ggcaattaac cttgcttgta gaagagcatg tgaagaaggg 32280
ttgacaaaat taaatggacg aaaaccagca attggtgcta ggcttttgct ggcataccac 32340
gatgaaaaca gttgggaatg cccagaaagt atgactcaag aagttaaagc cataactgac 32400
tggatgtatg gtcaggcatc taagaattta ggtttgaaga gtgagacgct tgtaactggc 32460
acaggcaagg ttggtaagag ctggttggaa gtacattgat ggtgttgaaa tgattacaga 32520
aattcgtctt aaggagctat tggactacga ccccgaaaca ggggtcttta cttggaagtt 32580
tagagaaggt aatgcaaact ttaactctag atttggaggt aagagggctg gaaactacag 32640
aacaaatact gatggacaca agtcagttca gttgctgatt gatggaaggg gttaccaagc 32700
aaatcgttta gttatcttgt atatggatgg atatctacct gataaaacct tacaggttga 32760
ccataagaat ggagacggtt gggacaatag gtacttgaat ttaagggttt gtacagcttc 32820
acaaaactct tgcaatcggt caagagagtc cccatctggg ttgccaaaag gtgtacagaa 32880
gataagtaaa aggtttcgag caatgatacg tttacaaggt aaaaactatc atcttggtat 32940
gtttgacaca cctgaagaag cccataaagc ttactgtgaa gctgctatta aactccacgg 33000
agagtttgca aaactaagtt gacaaggtgc tcatggaaga gtaccttata ctatatctta 33060
tataggaggg cttatgagcc accgtggaag aacccatgca gcgatgatga aaggtgcttc 33120
aaaagagact atcaagaatc gtaagcagaa gttgtttgag aggatgaaca ggttaattga 33180
caattcatct ctttcaggtt ctgagaaggt tttcttgaaa ggtaacctta aaagcattgc 33240
acaagaactt attgatattg agtattggag gcatcaaaaa tgacacttgc agacgttatt 33300
cagcaacttc acgacaactg ctatactcca gagttgattc aggagatgtg tattgtagtg 33360
atgcctagca agttcgtaaa agcctttaac agtgacactt taaggtctgc acacattctt 33420
attgttgaag ggaagattgc aaaagaccgt actggagtac tcaaaggtga acgtattgat 33480
attctggagt tgctatgaag aaaatgtaca gtctctgggg gagggttggt aaaggttttg 33540
actggacact tcttcgttca aacgttaaac gtagtgaatt accagaactc atcactcact 33600
atttaaaaac atacagagag gtagattatc gtgaacaata aggttaagag ttgtgtgaaa 33660
gcaatggttg cacttggagt aatttttctg gctggctgta acccatctta tgaaaacaaa 33720
gatgcctctt acagcctccc tccagagatg caagactgca aaatctacaa actcaatggg 33780
gatgctataa gcagggatat tgttgttgtc agatgcccaa actctcaaac aacaacatct 33840
tatagatatg gcaaaaatgg tcaatcacac actactgtta ttgatggatg agactaatat 33900
gcaagtttta gtacactata tctactgcta cgatgttgtt tattccactg caaccgtggc 33960
ccaacgtaac ccaatcgtac cgcgagaagg tgaattggtt cgcattgagg gttggactta 34020
cactgtggaa agcatcattc ataagtttga tgttgctggt gatgttcaag ttatcgacgt 34080
ggaaattggt ggtaagagaa aatgaaatct aagcaaacaa tgacagatgc aatcttcaca 34140
gtacacagtt tcgaaaggtt gcacattaaa gatgtaaaaa atacaccaaa atgcgttgta 34200
catttcatta aaagacgtag tgagaggtct tatataaaat ctgacacagg cgagagtaca 34260
aatgggtaaa gataataaat ttcagaacgc agttcttaca gaagatggtg agcttgaaac 34320
attcattctt cgtgttgatg gtaagttatt ccggtgtcgt tgtgggtcaa actgtttcca 34380
taaaccagat aaaaacgact tagagcttta cgcatgtaat gcctgcaaca cttggtatca 34440
ctcagaggtt aaaaatgaca attctgtaca aacaaaataa agatggttcc ttcaacgtct 34500
ggtcatgtgt tgctgtaggt gacaaagtta tcacaaccta tggtaaagaa aatggcaaga 34560
tgatgtttga agagtacaca gcagagccta agaacatcgg taaaaagaat gagcgtaatg 34620
ctgaacagca agctctcttt gaagttgctg ctaagtataa aaagcaagtt gaccgcaaag 34680
gttatgctta cacaaaagat tctgcacaga atactgagaa ggtaggtgta cagcttgctc 34740
atgatgctgc aaaggttagc catgcaaagt atctgaagtt ccctgctgat gctcaaccga 34800
aacttgatgg tgtccgttgt aggatttcaa ggcacttgag tattggcagt gtatttacag 34860
catattctcg tgagaatact gtttacaaaa tccctactga actaatacct gacattatgg 34920
caatgttaag gctccacaaa gatgttgatg aatttgatgg agagatttat gctcatgggt 34980
gggacttaga agatatcgtc tccatgatta aaaatgaaga caatcctgac agacacttgc 35040
tcaagtttta ctggtacgat atctgtgaca gtactaagac gtggcctgag cgtagagata 35100
ttattgagac ttcaccattg aatgacttta gagacggctg taaagttgtt cctgttaagt 35160
ctcgtcgtgt aaattcttgg gaagagtttg atgaagctca cgataagtgg gttgaagcta 35220
agtttgaagg tgcaatgtac cgttcaatct ctgaagactc cttctatgag tgttgtcacc 35280
gttcatactt cttgattaag cacaagaaga tgcacactga agagtttaaa gtgactggtg 35340
taaagactga taagcgtggt catggcaagt tcgttgtaga gactcttcct aacgtctttg 35400
tagatgtctc atggaagact actcatgaga agaaacagta tcttgctgag catcctgaag 35460
agtttatcgg gaagccttta acggttcagt tccagaagat gactcgtaag ggttctttac 35520
agttccctgt cgggctagct attagggatt atgagtaaac tatgttcagt gcagtcattc 35580
tgtttatact ggttgtcttt gtattgtcat acattgcaag taagcttttg gacacatctt 35640
ttgcaatggg tgttttgttc acaatcttct gttttgtaac acatatagct atcaggactt 35700
ttatataaaa agttgttgac atagaaaatt tggtgagtat actgagcagc ataaaccaac 35760
gggtactcac cagcatcact taagagtctt ctaagagggt ttttaagtga tgtgaggtat 35820
ggcagtgttc tgttcggtac ggtgtggcac ggtaatgtta ggtctggtct ggtcaggtcg 35880
ggtcaggtgc ggtacagtat gggtagttag tctcagctac attaaaatga gacttacctt 35940
tttaaaaggt ctttgaagag ggtcttttaa taaggcgggg tgcggctcgg tctggttgtg 36000
tgaggttcgg tttggttagg taaggtatgg gtggatggta gtagccacgt taaaaatcta 36060
ccaagtttct aaagagtctt ctgttgaggg ttctttacaa acaacttaac aatcaactaa 36120
agtgtggagc taaatgctat gaaactgtta aatatcaaaa tcactggtac tcgccctttc 36180
ctgagccacg ctgatacact gtcagaccca ttaaaccctc tgacaaaata tcacaagtct 36240
ctgtctagta aacgtaaaaa gactgacgaa gactatgcgc tcttagcaga aagtcaactg 36300
gtaacctctt gttactacga tgagcagcta ggttttgtta tgaacggtga gatgattgag 36360
gcttgtatca agtctggtgc aaaactcaac aaacttggta aggtgattga ccgagccatt 36420
atgctgacag atgtagtctt cccaatgact atcaagaact gcccagcaaa cccacaagaa 36480
cttgctaaga atccagactt tatctatgct aaatctgtta agattggcac agcacgagtt 36540
atgagctacc gtccaatctt ccgtgactgg tctgtagagt ttggtttgat gttcgatgaa 36600
gagcaaatca ccaaagaaga actcctgatg gttctggaaa atgctggcaa cctttgtggt 36660
gtaggtgatt ggcgtccacg ctttggtcgt ttttctgtag aagttatttc agaaggtaac 36720
gtttaattat gaatacaaaa gcattagcag caaagtttag ttatggtgac actgtatcac 36780
actctgagat ggacaccttc ttaggtattg tgaagccaac ttatcaaggt gacattgtta 36840
agtacgaaga tgaaatgaag gcttatgctc ttacacgtct aaaccgactt gagaagttta 36900
ttgagaagct tcttaaagaa gagaagattt atctggttgc ttcaatgggt gttggttatc 36960
gtgtggttga acctaagcat caagcaacta ttgcaaaacg taaaatgtct ggtaaaattg 37020
gtcgtgcttt aaagcaagct actcaagcaa ttgagaatgt gaacactatg gcacttagcc 37080
accaagaacg ttcacgacta attgaacagc aaaaccactt agctgctatg aaagctaaca 37140
ttaacaagca acgcagaaaa cctttctaag ttgactaggt agcctcttcg gaggctcctt 37200
tttaaaaggt ctttaagagg gtcttttaac aaggtattgt atggtgtagt ttggttaggt 37260
atggttaggt gtggtaaggt aaggtatgga aggatggcag tagccttata aaaaatctgc 37320
caaaataagc tataaaatct gttgacaagt caggtgttat agcttatttt tatactaaat 37380
gtaatacaaa attcaatata actttgaggt cattatgaaa aagattttat tagctgctgc 37440
aatggttatg gcaatgaatt taccagtcaa tgcaacagaa cttccaaatg tggacttatc 37500
aggtgttcca gaagacactt gtcagattgt taaaggtgtt gccctatcta aaggtgaact 37560
acttaaacca atctctgaag agtctttaac agagatgact gataaggtaa ctgactatca 37620
gtatcgtgtt cttgcagagt atttcctgca atctgcaaat attaaagaga agcaccctga 37680
tgatattgat gtacaggcta tgctcaatca tcgtatccag tttaaagaag atttgatgca 37740
aaaagctatg tatggtgttg agtatttctt agaaaacaga agctgcacag gtatttgata 37800
tggctcttaa aaagttacat cccagaagcg gttatggtaa gataattgac gatacagacg 37860
gctttacagt ctttacagtt atctgtcaag atgattcaca gattgaaaag gctcttgacg 37920
attatcttaa cgatgaacgt gaaaaggtta gggctacaaa catagactca ttaattgaca 37980
cttcacgcaa acggaagaag aaagatgaat gaggtttttg acccttatgc cccacaagat 38040
gattgggagg ctgatagaga ggctgaaatg gagagttata tttgtccaat ggatgtagac 38100
gaaatgagag acttcgttgc acatcgtttt aagagagaga ttaaatccag aggtctttct 38160
caagagcaag ttgctaaaat ttgtggtatc tctcaggctc gtgtatccaa cataatccat 38220
ctcactggta acgtctccct tgagtatatg ttggaagtat gtgaaaaatt tggtgttaat 38280
ttcaatttaa ggttggcaga ttaatatgaa acgtgaaaac attatccact ctgaaaactt 38340
cgcattaggc ttttatggcg tacctactca ccttgaaaaa tattatggtg tgaagattct 38400
ctccaatctc attatggctt acaaagatgg taagattaag catactgaga agaaacgtgt 38460
catgggctat atggctgtag gttcagcaat ctcgaacatt aagctggaaa caactagcag 38520
tcagattgtg aaagaccact tcatcaaaga gctttaccac aatcttgatg gtgtagatgt 38580
tcaggctgtt tggctggatg ttgatggtca taactacaca agttttgttt tcaaaaacga 38640
tgacatcaag tgtctgttcc cataataggt gattaactat gattgatata ttcttacaag 38700
atgcccatgc agatttcctt aaaacgatgc ttaaaaagtt tatggcttca cagtatgaga 38760
atgaagcatc ttttaaaata gttacatgtg gcgatgaagc tggttttgtt gagattgaac 38820
atgaaggtac tggaaagact gtttgtaagc tacctgatag catgttctct aaaacgttct 38880
taacaaagac tagtataaat gttaagcttg ttcctcagat tgaaacatac tctggtacag 38940
attaccctaa aggcttcaag tcactgatga aacacttctt agatgacttt gtgagtaatc 39000
ttctaagtga ggtaacagaa agccgtacaa tattaactgt agagaatata gggaacacta 39060
tcagggttac ttctgaccga tattctatga gcctcttcga ctttgtacct aagaactttg 39120
atggtattct ggatgaagaa gatgactgtg tagactttat attggttctt gaaccagttt 39180
ttgaggttaa atagatgaaa attgaacact gctatgagtc tgatggaaca cctatccgtt 39240
gtccacattg cgggtgtaca gacttacaag gtgaggtaag tgaaatagtc aacggtcata 39300
ttgctgaaga aagtacccga tgcacagggt gtaatgaaat tatcgctttc tgggcttatg 39360
gttcatacca accctctcca cattttatct accatcgtag taaagttgtg aagagtgtta 39420
tcaactggtt cattaagaaa ggatttacaa aatgattaaa ttaatctttg caagtggtga 39480
aaatggtgag tttggtactc caactggtat gccgtggcct cgacataaac aggacatgca 39540
agagtttaag agactcacta aaaataactt agtagtcatg ggtaatgaga cttttaagac 39600
tctgggtagt aaacctctac cagaacgtgc aagcctaatc ttaacaaatt ctgtaccata 39660
cttaggcata gactttggca aagatgatgt aatgtatgct aaagccagta aagagtcatt 39720
tggagcattt ttgaagtatc ttgatagctc tattgatgaa gatgtctttg taattggtgg 39780
tgcaggtgtc cttgtcaatg ctttaccgta tgctggtgtg gtgttccata cagtcttcca 39840
taaagttact gaagaggcca ctgtgcattt accttttgaa aacttctttg agaagctgta 39900
tgataaccgt gtatttacaa aggtacagtc aaaaccatca gaggatggta aagcaacttt 39960
tgaaatttat gttccacaag taaaaggaca cttttgatat gtcacaagca gattcaagtt 40020
acaaaaatat cctgaaccat gttttatccg ttggtgaact gcgtactaca cgaactggag 40080
atgttatctc tgcatttgct ccacctcagt ttcgttttga tatgcgaact ggtttcccgc 40140
tcttaacatc taaacaggtg tttacacggc aagtcattgg ggaagcttta tggttcctga 40200
atggtgagaa taaacttggt gaactccgtt atcgcacttg gggtgaaaat gatggagaac 40260
gttggactat ctggtcagat gattttaaac gttggttaag ctctaactat tcttctgagc 40320
aagactggtt agaagatgca ggtgggagaa tctacggggt tcagtggaga agctttgaag 40380
gtcataatgg ttgtgttgta gaccagttag aaaccttagt aacgaagatg aaaggtgata 40440
tcacagaccg ttacatgctt gttaatgctt ggaatgcagc agatattgca gcaaactcaa 40500
tggctttagc accttgtcat gttctgtttc agatttatat caccaacgaa ggtgaagttg 40560
acttgcaatg gtatcagcgt tctgtagaca cctttttagg gcttccattt aatattgcgt 40620
cttatggttt tattctggaa gttctttgta agatgactgg atacactcca cggtacttga 40680
taggtgtctt tggagatact cagatttatc agaaccatat gaagcaggtt tatgaactga 40740
tgaacaatga agagttccat gcacctactt ttgagattgg tgtacagctt aacactttaa 40800
gtgacttaaa acatcttact gcaagtgact ttattggtgg aattaacaac taccaacatg 40860
cagggaagat tgaagcacct ctgtcagtag gcaagtaaaa caaaaaaggc tcccgtaaaa 40920
aggagcctta aactttattt ttcagtattc tttgtgttct tttcagtaat agcttgcaag 40980
gctgatactg attgagccag attattcaca ctgtcagaga atttatcaag agttttggta 41040
agttttgcat tctcaccctt aacgttctct aactgaactt tctggttctc catccctagc 41100
tgaatcagtc tcatgtcaga ctgtaaatca cgaatagctg aatagttact tttggaataa 41160
ttatctagct gctgtaactt tgttgtgaca gacacttctt gtttaccact tgaaacctgc 41220
atcgtggtaa acatcccaat aacactaaaa ataccaacta caattgcacc aatattattt 41280
ttaaaagctt cctctagcca cttcatttat ttctccccct taaaagcttt ttctaagtta 41340
tctacgaact catcatcaat aggtgtgtct gttttactcg caaggtatct tgcaagctta 41400
aagaacactt tctcaatcat gtattcactt agaagggata aaatgagttt ccagaagaag 41460
ctacctagat tttttagaag aattgctagg attgtaggca tttaatcacc tcatcagcca 41520
agatggtgag aatacccata aagaatatta acaccatctt aacaatcagt caacaaggga 41580
ttaagcagtt cttacccaag ccattaactt gtagaactgg ttagtaacac taaatgccga 41640
accagaacct gtgctaccag tgttaccact aactgtgtgg ctatgagcac caatacctac 41700
agagtgggaa tggtcaccag caccgcttgt agtaccagac cacgtgtgtg tatggttacc 41760
agttgtgtta gtgttaggga acttgttatc tcttctatca agccagtcaa gagttcttgt 41820
cttgctgttg ctaacagttg tgtaaccata ctgagcatca ccaccattaa ctaataacag 41880
acctaagtgg tgagcgtggt tgccagcagc acttgttgta ccacttacag agtgagaatg 41940
accaccagta ctgttagttc ccttcgtacc ataatcaaaa gatgaagtgg tagcagagaa 42000
actatgagtg tgtgatggca agttaccaac agacaacgtc acagaatctg aaccaccagt 42060
tgtagcaaca tctgaaccgt ttgctgctgc aattctgata gttctaccaa caccattgtt 42120
caggtacgtc caagttaacc caggaagtgc tgtattaggg tcaacattac tgttaaacca 42180
cgttacaata cctactggat agattttatt aaggtctgta gagtcactaa ttgctgttgc 42240
aattttctgg tcagtttcag ctttagtgta tgcaccaatc tctgaagggg ttggtttttg 42300
tgcttccgtg taaactttag accaattaac ttcgaagcca taggcatctc ttgatgttct 42360
ataccacatc ccaccatttt taaacgaaaa ctttaattgt gcagatggtg tagaaccagt 42420
ccctaaaaac atgtgatata caagtgaaga agactgtcca gtggtgttag taaccttata 42480
aagacctgtt tttgcatccc atgcaacacc atcaccacca tctgcttcag aagccgaacc 42540
agtgatgctt gcaagcatat atcttgcgtt tgcggcagat tgggtatagt atctagcatc 42600
taagttagag aagtcagaag gttgcacctg tccagtgatt ctgactgttt tgttcagtaa 42660
gaagtcattc accatgaaaa tggtagtagc agtcctatca ttgtggataa cgaaactttc 42720
tggtttttgt tccccgttac cgatgtacca ccttctaaca ttatcagcat taaagccagc 42780
aaagtataac tggctatctt gtgtagtatt ctttagctgt aacaataagc cattttgatt 42840
aataatctgt gcagacctga aagtgtttac atcacttagt ttagcaagat tactcaaagt 42900
agctgctgga atgtatcgag aatcaatatt agtccaatca gaaggttgca cctgtccagt 42960
gattctgact gttttgttga ctgtcaccaa attctctaaa atagcaattg aaacgtcaga 43020
ctttacattc ttcaatacaa gattatttgt acccctatta tcattaccta gataccatct 43080
gttagtacca tctgcatctt gaccacgaag atagagtgga gcaccttgag tgatattttt 43140
aataatgagc gcttcattgt cagagaggat agcttgagta cctctaaagg tattattcac 43200
ggctagtctt gcataccttg cgtcattctc ttcattagtt ctcatcccaa gttcattagg 43260
gcttggctta ttcaaagtat gataaacagt tgccgacctt gaagcgtctg caatagttag 43320
tttaaggccg tttgcgttga ttttaaaagt ctttaaaaca tcaccaacag taatctcaga 43380
agagcctgtg gggtcgaata cagccttgcc accgtaaagt agcttaacaa ttcctaagtc 43440
acccgtcata gtgctaccag caatctgaac aaatctttca agcttaaaag atgctaagaa 43500
atcttgatag gtcattcgac ggtcttcatc acccagcatt ttaggtctct ttttaactct 43560
gacatgaaga aggtcatctg aacggattgt gtcgattgag tttagctcac tcaatttgta 43620
atctgccatt ataaattttc ctcttaaaag gggctataaa gcccctgtaa agaaattaag 43680
ctgttctctg ccatacatac agtacgaagg atggctgttc aatattaact gcctgacctc 43740
caccaacact atttgtattg ccactatgtg tatgttcaga agaagaaact gttacagtgc 43800
cactatgcgt atgtgaacca ataccaactg tatgactgtg agcaccaata ccaaccgtgt 43860
ggctgtggtc acctgatgca ccagatgtga cgttaaagct gtgtgagtgc gcaccatcac 43920
tagaagtcct tgatgtcctt cttgcaccac caccatcaga gtcagccaca gcattaccag 43980
tgccatcatt ttctctagga tacacatcat ggttgtgagt accattggtg ctagtgttac 44040
ctgacacact gtgtgtatgg ttaccagttg tgttagtagc ttttgtccca tagtcaaaag 44100
agtttgtagt cttagtccca taatcaaaag ctgaaattga cacagttgca ttatgagtgt 44160
gaccaccacc gctaagagac acagagtggg agtgctgagg catgttatta actgacaaag 44220
tgacagttga agcgccaaat acagaacctg ctggtcttgt atcagagtca tagccaacca 44280
atgccctgcc ttttgaaact aactcccaag tacctccaca aattaagtat gtggaagggt 44340
ttgcagagtt catagaaaga tggatagtac ctactggata agaagcctga acagccttgt 44400
acaagttatt tactgctctt gctgtagcat acttatctgc atcttcgtta tacagattag 44460
atgttgtcca gttctgaaca ttacttaaac caacctgtgc cttagttgta ttgtgtgggt 44520
tactcttgtc cttgatatgt tgctggacaa ggttattaac ctcttctgaa gacataatct 44580
gtagatttgc tcttgcctca gctacattaa caatatctga taagttattt gcagcgacta 44640
actgaagagc attaataaca ttatctaaac caatttgtgt tttagtaaca ccatgagggt 44700
tatttcttag gcttgcgtgt ggagcaagta attgctcaag agtacacctc ttatcctcaa 44760
taccctgctt aagatggaag atatcactaa ggtcaattgg taaggatgct tgaggcaagg 44820
cactaatttg aatttcacct actgccatta ttaagctcct acaaattcat aagtataaag 44880
attttttgta cttgttgatg cagtacctgt ttcggtctta ataagcttcc agccattagc 44940
caccaaatct ggttgcgcag tggcaaaaga ccttacctca ccaacagtac catttgcttt 45000
cttcaggaga tagtttaaga tataattaaa ccattggcga cccattggtt cacctcttaa 45060
taaaccagtc gcctgaattt ctggtggtgg taatactttt agctggttac catcagcatc 45120
tacttcatct gtagaccaat ttaaaaatgc cattaagaac ttccttcttg ctgtgattta 45180
tcttttctac ctttgatgat ttgagctact tcagccataa caccataatc accacctgct 45240
acagtttctt taccaacgat gtagttgtct gtagcattat aatttttatt aaccttcaag 45300
taatctactg agccactatt agccgttcta tcaatcttga aataagcatc tcttacacca 45360
gcatctgcta aactccctag taagttcttc tctaaagaac ctcctgtatt gttggtaacc 45420
agacccttgt tagcatctgt aacaagccag ttatctttat catcaacaat tgctaaagca 45480
gagtcagcaa cctctacagg tgtccaagca gtaccattca gggttacatc tcttagaatg 45540
actgctgaac caatagttgt tgcagcaatt tttgctaatg tgtatgctgt gtcaacaacg 45600
ttatttcttg tgttaactct aaccacaata ccagcagtca taggtgtaat atgttcaaaa 45660
agctgtgaaa atgttgcatc atacagagtc atgatagcat tctgtaaaaa tgttggggtt 45720
gtatcagaac gtcttagaaa aatctgaata taaagaattg ctctatatgt ttcatcatca 45780
gcaccaagtg gtcgtggtac tttaattaat gcaccgatgt tgtcaagttg ctgtccaata 45840
gccttcctga tattcctttc agtgtgcatc tgccatgaaa catcttccaa tgtttgtagc 45900
tcatcagtga tagctttcag caaactcgta tagatgaatt tctctttaaa ctgtgtaaca 45960
gtcctttcat caagagtctt gtaataaaca tcatcaattt tctgaaacat tatcactcct 46020
tagtaatggt gtacttgcta ctttcccata cagtgtattg gtcaccatca acagtaattc 46080
ttgctgtagt gtactgtcca tcactaggtg gtactgactg gctattggat agtgctactt 46140
tgatttcatt aatctcgata cctttaatga cattataaat atacccatag attctattag 46200
ggataacatc attaccaact ttcaaagttc taccataagc gttaatacct tgaataatac 46260
tgtctctgat atcttcttct ggaattgtca agctttcttc attatataga gagtatgata 46320
cttttacaaa agcatactta ggtgttggcc tactgaagta aatattatga gatagtccac 46380
ctaagtcata agctgtccca aagatagccc cataagctct aataccagca ggtttagtgt 46440
cccagattgc ttgagcaaca ttatcatttt gaccaccaac tacaacaatc ttgaaagatt 46500
ttggtgggag tccttctgag cttgtctctt cagtatcatt ttcaacacct gaagcatctg 46560
atacgccctg aaccctctta acagcagcta cgattgcatc cagagtacct acaccagtaa 46620
ctgacaggga ttctaaatat ctctgacgaa gttctgtatc ggtctcttca tttctacctg 46680
ttgtcaggcc ataacggtta tatacgctat caagaccatc tacagttgtt tcaatttcga 46740
taagtgtccc agctaatgca ggaattgcac caacttcctc agcaacaaca tcatggatag 46800
ttgtaatttt tgtgaatgtg aggaatgtgg tagcagtcac taccataggg ttggttcttg 46860
caataatgtc gccttcatct ttataaactc gtaatgctga accatcattg atgacttcag 46920
cttttgctac aataccacca ttaattgcat tagcaagctc agttaatagt actgtgattg 46980
tgtctgaaga ttttggctga taagagaaaa taacgttatc aataataata acataatttg 47040
catcagttcg taaagagtta acttcaagaa tagcctcaac acagtatgaa ggggttaatg 47100
taatgtttga aactggatag aagatattcc cagcagtact tcttaatctg gttgtagacg 47160
gaattgttgc acctgttgtt ccagtaaact ctacttgacc ccttgtagct tgagcaacat 47220
atctatacac agcgtttaaa gctgtaatat catcaaggtt aaaaccttca gctttatcaa 47280
tcgttccacc atcatagatt tctgatagaa tttcatgagt gtctgctaaa gaccttgcaa 47340
ttgaagctag aaagagacct aactgactat cctcagaaac gtcaaggttt ggtgaaatat 47400
ctctaagaag ccttgatttg atattatcaa aaatttcctg atatcttaga gtttgtaatc 47460
ctgttgtagt taatcctgcc attagatatt aacctcttgc gtaatgtctg ttaaaatatc 47520
tgttgtagta gttgcatcaa aattaacagt tacttttctc tgagcattat ccattgaaga 47580
agaatagtta tagatgttag atacatctct tgtttcaaca aggtaggctt tcatgtaatt 47640
atcaaagata gaagttttct gtttaaattt ggcaagttgt aaatatggga acccagcaga 47700
tgtattaaag aaaacttcac cagcccttaa aaggcatcta atatgaagtc tttgagcaac 47760
ctgagtagct ttatcatctt ctgggataat tctaatttgg ttaccagtaa tctttaaatc 47820
tccatgagcc acatacactg aatctgaacc taaagtggca acatagtcac cacctagatt 47880
taatgcaaag tctgttttca ttattgtgcc tctgtagtat cagcctcacc agcagggtct 47940
gtccagtaat aatggtgcgt gtgttcatta aagctcacac cagtcgtgtc actgataaaa 48000
tctgagccgt gtacttcttc tgttacgtac aagtttttag aaatgtgtac atcaccttca 48060
aagtagaagt taccatcatc agtaactctt aacacagagt caccgaaatg aagtctaact 48120
gctgttgggt ctggtttaaa attctgtgtt cttgtgcaga tgcctacgaa agctacacag 48180
tctgaaatat catgtgtcct tctcatgttt gtttccatct gaacattctt gtcattgaca 48240
acgaagtcat ctaaaggtaa cattgagaaa gctaaccagc atctgtcatt agtttttacg 48300
gggaatgtta aagatgcccc accaccactt ggaaattgaa caggtacacc agtaatctct 48360
ggcataggta aaccgttaat agagtaaagt ggcttaacag tggctgtttg agtctttgaa 48420
tcgaaagact gaataatggc tggtaatcca gtatacagtt cttttctaaa ttcatcaaga 48480
cattctgaaa catacccaga cattctagta actggtgact tcattattcc accttctcta 48540
aatctagttc agttgtccaa gcaccaccag taaagtcaag attgtgagaa agacctttta 48600
ctcgatactg accttcaaaa tcttcacttt ccctaatagt gatgttatca cccatcttaa 48660
ttctcccatc taaatggatt ttgcaacgaa ctccagtttt aactttaata actgtcttat 48720
tctctttttt cagaaccttt ctagttcttc tgtagtagcc ttgcaaagaa tcaataacgt 48780
tatatgggta aatttcccaa gaaagttgtc tagccttagc gttaaaagga actactcgga 48840
tttgcttgtt aaatgtatac caacgtagac tactttcttc acaaaccttt gtaagtgcct 48900
ctgcaacact tccccaaaca ctaaaaccat tcttgtaagt gtaactatca atacttgaaa 48960
ggtcttcatc aataagtgag aaacctagtc tgttgactaa atctctaatc acacttttac 49020
gcgttgtccc tgctttataa gaaattgatg tcttaatcgt ggttctttcc attttatcat 49080
tggaacagat aacctttgta atcatatcaa caccacgctt atatgtataa gcatattcaa 49140
tagtgcctag atagattaat gggaggttat catattcaat aataaggtcg ccatttgcgt 49200
cccttttaaa accagtagtg taacctgctc taagcataac tgttgcacca acggttttga 49260
atttggctct catctcttta ttaaggttgt agatttcaaa agtggtatca tcagaggtta 49320
ctttattctt ctgagacgta taagacacat tacaagtaaa ttgtaagttg tcaaaatagt 49380
ccatttgcat agaatcttta gcatggcttg taggtttatc attaaaggct gtagtttcac 49440
tacctacagc caattgatag cacctaaaag aagccccagc agtgctatct tttacagaca 49500
ttacaagttc tccattaatc tcatatcttc ttgagtgtaa taattaagct caaatgcctt 49560
ttctcttccg aagttatttc tggtaggctg taaatcagta ccatacattc gttcaacaaa 49620
aagctctcca gctaatgaag gaattacata gcgtcctgtg attgattggt ctgcaaggca 49680
tttcttttca gataataata cattaccatc aacatcagat agcgtcaaga accatctgtc 49740
aagcctctct ttatacttta attcaattac aaagacagtg ccatccagag ttacagtttg 49800
tgtagaccat cctgtatcag gaacaggaat atattgtgac attaataagt ccccttttta 49860
ttcggattca ctgaatgctt ttgtaatgct tttccatctc cagcagttct gtttaatgct 49920
gctccggcat ttttctcagc ctcttcactg aatgtagtaa caccttttct ggtttttgca 49980
gacatggaac attttgcaag ggcattatct tcagcactag taagttccct tactccattg 50040
gcatcaaggt cgaataataa ctgacagttt aatttcccat tacctagact tgttgtggta 50100
ttccctgtat tctttttact ggtagcacca ccatcattcg tggtagcggt ctttccagtt 50160
gcagcagaaa tatcagtttg tcccacgata gctttaaagt taatttcctg aaaagttagc 50220
tggattctta gaccatttga aataccaaca tctttagagg ctttaaaact tgtgataatg 50280
gaatcatcaa ttttaattcc gtctttacag atgactgaaa taatttgctt ctggtcacgc 50340
cagctttcaa gagtgtcgat gaagttttct actaattgac cctgacgagt taataataag 50400
cttccttcat agccaactac aacgacacca ctaatagtga ttgttctggg tgctctttgc 50460
acattatctg tgacggtttg ccctgattgc atgttctgtg tagttacctg catagggctg 50520
tcaaattcca tattttcagt tgctgataag gttaagaagg catctacatt atctcttaag 50580
tggaaataga tgccgtcttt gccactatat ttgatttgca tattagaatc ccataacatt 50640
attcttcctc tggatagctt gaacttagaa gaatgtcttc ttgattcttg tcagtaatat 50700
ccaccatctt agtagcaatt tgtttaccat caagattgaa agtaacattc agggtttgtt 50760
tagtctgcat aggtaaacca gaaggtgtca tcatcattgg tgtctggtta aacttattgg 50820
caaaattatc aatagatgtt gatagcttct ccatgataat ctcccaatta gatagaccat 50880
tatcaattaa ctttctgttg ccttcaacgt cttgagtgta ctgtgcaaac tgaagttgac 50940
cattctcatc aaagaacatt ggcctttttg ggtttgtaat atttgcaaca gcattttcaa 51000
atggttttgg taatgtaact tcactgtagt ttttagcagc atttgggtct gtagagcctc 51060
ttagcattaa tgctgaacca acagtaccaa gtgccattct cgttgctgta actccacctg 51120
ctgctgcggc tgcttctccg gctgctgctg tacctgctgc actaacacca agtctttgta 51180
agattttacc aaagatacca ccaccaacca aaccacttag gagcttgact gcttttgata 51240
ctacagcaac tgcgcctcca attgtgacaa ctgttcctag aaattcacca gcacttttga 51300
taagcttttg ctggctgttg tcaaggtctt tataccaagc tcttgcatag tagtatagtg 51360
cagatgttct gtacatgaag tctgtaatga agtcaagtag attactagcg ccttttaaca 51420
ggttcccaat cacaagacct aaagcctgcg tgctacccat agaaccttgc aagaacattg 51480
caacggagtt agataactgt gaaatgccct cactagaatt gttaaacagt gctacaagtg 51540
tgttatccca catagccttc gcttgaccca ttgatgtagc agtctgcttg gacacagcat 51600
tcataccacc tgcttgcttg acaagttcag ccattctttc agatacttta ggaagaacgt 51660
cttgagcaag aagtttaccg tcttgcatca tcttatcaag ttcttgtggg gtcttaccaa 51720
tagcatcagc gaatagctgc acagcacctg ctaaacggtc acctaactgt ccacgaagtt 51780
cttcagcctg aactttacct tttgacgcca tctgctggaa tgcaaccatg atacctttca 51840
agtcttcatc agtagcaccc ctgatacggg caaacattgc agcattctta tagaactctt 51900
gagtaccctg aaagccaagt gttggttgag caccagcagc aaagtttgag tactgcttca 51960
tggtatctgt gtagttctga ccaatttggt gtgcgaatga tgcagcgaac attctagctt 52020
gctgagtgtc tgctccaaag atagctgtag aggctaattg tgcagattgt ctttttacac 52080
cagcttcaat agttttttgt gagagttcca gtaaagcata agctgaaaca aatccaccaa 52140
ctaattgacg taatgatgcg ttagctctat cctgtagcca agctgattct ttaactgatt 52200
ttagtctagc attttctgca ataacccaac gtttggttac gtcgatgagc tttttaactt 52260
ccatctcata ctcaccaacc ttaccagtac ctttatatct gttatagata ttttgcaagc 52320
ttcctctgaa agaggctgcc atttggttac cttgaccacc gattgtttcc agtctacggg 52380
ttaaccctga atagaagttg ttgttaaaca ttctttccat ttgtctctga gcaacatcta 52440
ctctcggacc tctgggtgct ccaccaccaa caggagggat actctgtcca cctctccctc 52500
tccctgtctt gatagtgatt ttaccgtcaa ccttcatagc atctcttaat gacttgttaa 52560
taccttttgc agtctttttt gcctgagttt caagtttctt aagagattta acaccttgtg 52620
aatcaaggtt caaggaactg ttgagtgctt tattgattct gcccgaagca gactgagcat 52680
tttttacaat tctattaagt gcttcctgag aacttttatt aggtttcaca tcaaaggctt 52740
tattgatatt tcgctcaata cgctgagcag cttgcataga cattttctca actctttgca 52800
agcccttaac aaccttttca ctgaaaccaa gttccacaat gaagctatca actgtatatt 52860
gtgccattac atttttcctg ctcttctaag ttcgttgtaa gcaatttcct ctttatacga 52920
cctctgaatt tcaagaaatt gtctcaatga taataaatca gagaatgtca tagcaaagag 52980
ttggtcaagt gtttctttac acccttccat accataaata gcaagcacaa atttcatctc 53040
gtctgcttct tcataggttg cctctacagc agcatcagtt agtggtgtct gtagagtatt 53100
acccatgttt actgagaagt taggcttttg aaaatgcttg cttcgaaaaa acttccgaag 53160
tttgcctcca gcgcaaatgc taagtaatca ataaactcac cgtagtttgc ttggaagtat 53220
gtatcaatat tgagtgggaa gtcatcaaca gttgcccctt gaaataacag ggtagccatt 53280
tcttcaaggt taatttcttc aattctgtca aaacaagctt caacaaggtc tttaaatgga 53340
accattggag cttctttctt atctttatca gtcagacttg atagcatctg tgcaaatgtt 53400
ggaacaacaa ttttacccag cttcatagac atcttaatcc catctcttgc cccaagcaga 53460
acaatattta ctttcttacc attaattact ctagattctg ttttcattgt gattccttaa 53520
tacttttaaa agaaacaaaa aaggggaaga ccttttaaag tctccccctt ataggattta 53580
ttaaacactt gacgctggaa ttgtagaagt gtagtctagc ttctcacaac caaaaatcca 53640
agttttagag ttctggtcac gcccaagttc aatctgtggt aattcctgca accaagcatt 53700
aataccagtt gccagaacag agcctgatgg gtcgtagatt acgaagttag aagagatatc 53760
ttcttcaagt tccatgttgt cttgtttagc ttgaattgca gaaagcatct ggttagagag 53820
agaagtctgc attagctcaa tctcaatagt acctgtcttg tctgcatttc ttgtcagagc 53880
aacctgacca cctgcaccta caactggtgt gataagtggt gatgttctct gtagacgtaa 53940
aaatgagtct ggggcaaagc cttcaatggc aataccattc cagctacata caacgtcttt 54000
aggggaatat tgctgataca tagccattcc aatttacctc tattattcgt aagccactgt 54060
acctttcaag tcaacatcca agatagcccc tgctaagata cctgcgaagg taacatcttt 54120
caggatacga gctttcttgt ctgccaaagc aacttgagag gctttaggaa cattaactgt 54180
gtaagatgac aggaagtttc tgttgactgc tctttgtaga gaggtttcaa tgacttgacg 54240
aatacgggta ataccagtat catcataagt aatcttacca cccttctggt taattagcaa 54300
gtctctcaga gaagttttca ggtctgattc taaccagtca acaccacgga cgatatcaat 54360
ccattcccca ccagaagtaa tccctctacg aaccactgga acaccaccat caaggtcgat 54420
aaagttacag tgacgtacat ctaaagctga cttctgaatg cttgtcagag gtctcttatt 54480
agctggctgt aaagaagcag ctacaccagt caactgagca ttaccccaag caattgaccc 54540
tgcatcgtat ggagcgccat aagcaatata tgccatctct ggataatctt ctgccgctgt 54600
gtgatgccac aagcaaactg tgcgagtata catactctta gcaagctgtg ctggaacatc 54660
atttgcacta tttaattctg taccttgcag tgctgctaca tcagagttag ctgtgaagaa 54720
gattttctta cgagcctgaa tctcagaagc cattgccaaa acaaactgtt gagttctgtc 54780
ttctgctgca atgaaatacc agtcggtaga ataagcttca atagctgcca gagcagttga 54840
cgctgtatca gcagttgtgc ttgcaataga cacagtttgt gctgtagtgg ttaccttcac 54900
gaaatcattg tcaccagctt tggagatgac cattgtggca gaaccgttgc taccagtaac 54960
attgactgaa actttatctt taattgattg gtcagcttca atctgtgttt taaactgttg 55020
taatacattc tcggcagtat cttcaccttg tgaggtgtac tggaatggtt gtgacattcc 55080
cccaccgaca gctacagtaa ttgagtagtc cgtgctctca gtaacctcat caggaattga 55140
tacagtgtac tgcatagcgc atctaccaat ataaagctga gttactttag gagtctgact 55200
ccaaagttgt ttagcagcct tatatgcagc agtattctca tcgaaatctt cagcaacttc 55260
agttaaggaa gtataaccac gcactctttc ttcaaagtta tctgttgaag ctaagaatag 55320
tggcaaacca aaaccttctc ttgtagttcc tgcggtgttc aatgtaatat ctacattaac 55380
aattggattc cacatttatt ttaccccttt ggagtctaca tctagatgga tagtatattc 55440
tggtggctct tgtccttctt cataaaccaa ctcgccatca acaatgacac gctcaataat 55500
acttccgcgt tcatctttca ggactgaatt ttttacaaga gttacaacaa gaggcgcaga 55560
attttcgaaa tctgtattga gataagtgta gtcatttggg atggctccag tgtctagcac 55620
tgtagctcct gtctcttcaa gaattaaatc cctgactgaa ctcatctcta atcgttgttt 55680
aagctcaagc ataatactgt gagcaccttt cccatttacc gtaattaata caggaatctg 55740
aaaagcaatt ctgtaacaaa ctacatcatc ttcaacaaac ttatcaagaa cccaaccata 55800
aggtgttgca gcatcttgac aatatacggt aataaatggc tggtcaggtt ttaaaccttt 55860
gtcatttgag ttatcagaag ggtaagctct aattacgttt ggtctattat ttttatcacg 55920
agctaatctg tgaccaataa catccactaa ggttctaact agaccttttt caagttctgc 55980
tgtttctaac tgcattcatt ttatcccttc taataatgat atattcataa tgggatgtat 56040
gggctaattg ctgtgaccaa tccatagtca taaatacttc atactcatga ccatcaatca 56100
taacaatatc agactcattc cactctacat catctgaagt tctaagttta tatgtagtat 56160
acaggattct tgtatcggtt agtctaatac cttccggtaa agcaatctgt gtaccattct 56220
ttacagaacc tttgatatat ggctggatat tacctttaca gttaacctct acaatatctt 56280
gtgaagctac ccaatcacca tcatcattat aataaccgtc ttcagagact ttacgcttta 56340
ctacaaagct gtgtctgttt aagagtctca tttcttaata cccttcttag tagaaatttt 56400
ataagcaagg ttatccctta agtcacctgt ctcaacaaga ggtgcattaa agcctttttt 56460
cttgactgtg gaaggtgcgt taggagggag gatagcagaa ttaccaaaac ctcttttaat 56520
tgccttttga gcattctttg caaatgcttc taaggtatct gaggggtcta cgttgagact 56580
gctaagttgc ttatatagat tcttcttagt ctgctctaac aaggtctgtc tgtttagcat 56640
catagtgatt tcaaacaacc tacgatatac tttaccagaa gctgaaggaa ccccaataac 56700
ttcttgtaaa tacattaaag caggataaga aaaaccagag ctatgttgac cttgttcttg 56760
aaaataccca acttgagcat tagccgtttg caagttcttc atagccccga ctaattttgc 56820
tctagcgggg tgaataaccc ttttaaccat tattcatctc gctcgataat aaatacgcca 56880
ttaacacggt tgcagtaatc tctgccttcg tatctggcag catcaccata ttctgtgtat 56940
ttcttgacag agcaaggatt ctgacgacgc atatcaatgt cacactgatt aataccacct 57000
gcatagggta gtcctgaaac agagctttta acaaggtcgt cataaacagc tttcagagat 57060
ttgaatctgg aagagttacg taaataaaca ccaccaacct tctcatctcc catcttggct 57120
acttgaaaga gtaaatattt aagagctttg atggctgctt tcttttcatc ctttccagat 57180
tctagataga accactctag cacagactgt tcaataagaa tttcatcatt attggtgtct 57240
gtgcagagga ttcttactct atcaagaggg ttattggctg ggtcgcctgt ataacacatt 57300
cataaccctc cttaagaata ttagtcttta gcgtcagcac gaacatctac cagcaactga 57360
ggacgagtac agtatggcag catgtaagag tgagcttcga agtcaatacc ttcgtcacgg 57420
tctttttcat attcgaatac gtacagttcc tgaccaagtg tatttgcgta acccatctta 57480
ggacatggac cataagccac ttcgaagatg ttgtttgctt caccaagcat agaaacgtta 57540
gggaaagcgt gtcctacacc aacctctgca ccagcaccat caatgctcac cagagtatga 57600
accttaccac gcttgtcttt gaacttaccg ttgtactgga caaacttaac accaccgtag 57660
tagaaggtgt tcatgtgagc ctgaacgccg tcagtaccac cagttctcag agaaccagta 57720
atctgttgcc aagccagcgg agtctgctgt gcaagataag cgtcacgaat cttaggatgc 57780
ttaaccagtt tgctgaagaa tacacggtca acaactacgt gaatttcttc accgttgatt 57840
acagtgccag tcttagcttc gtcttccatg tgcatacgca gttcttcaat agaagcgtcg 57900
atgtcagcat tagggttgcc aaggtcgaag taaacagttt tcttctcaac gtcgaactgc 57960
ttgtacaggt cagcatacag agtaccacga gcatcaacaa ctttaccctt cagagcttgc 58020
ataaacagga actcacgagt aatatcgaac ttggtacgaa tcttcatcag cttcttagca 58080
cgtactacag cttcagtagt cagttcgttt gcagtgcctg gctgacgtac accctgaatc 58140
tcatcaggag taatgctttc aacttctttg aaatacatca ttgggaagct gatttgacga 58200
acacgctcag gtgcgctagt ctctgcttta cggctatcac ggtctaccgc atcaagcaag 58260
ctaacatccc aatcagtcaa gtccataagg aaagtagttt gggtgattgg tgctgaacgg 58320
aacagaccta agttggaaat atacccataa gtatttggga tagactggac ttcaccagtc 58380
aggtcagcaa ggaaaaatct gcttttttca gaattagtca acattgtaaa attctccaga 58440
atgtcttatt attgttatta caggccagtt ggtacgaaat caataccttt agcagccaga 58500
gctttcttga cgttctcagc attaacacca gactcaagag taagcatgtc tttcagttct 58560
gcgtcacggt aaataccaac aactttcaac tgaccgtggt aagacagttg caggtctgaa 58620
taaaagttaa cgatacatac agaatcagcc tgagcttctt cccctgctgc gactttagta 58680
ccgtcttcct tcagaacttc acctacacga tattctgtag atgcctgtgg agtgtactct 58740
ttacgagagt ggcctgttgg ggtaacctgt tcccaaagaa tgatatcatt cagaggttct 58800
cttttaccta acttagtaaa atcttgatat gccattattg tgttccttat ttgataagag 58860
attttagagc attctggaga gccagtttgc gttgttcagc ggtgtcttcg gaagcattct 58920
tagctggttc tttttcttct tcaaccaaat cagcttcgcc gtcattaccc atttcttcca 58980
tagcgttgga atgttcaaga acagcaccag cagtttcttt cagcttggta atttctgatt 59040
ctttttcttc cattgcagaa gcatgggacg cgatagtttc attaagtttc tggttagcac 59100
cttccatagc attcataaac agaacactca gagggttatc aagaccagca cctaaaatag 59160
tcgttgcagc ttcttttgca tcaaatccaa aagcttcagc agaagcagaa atcttattag 59220
tcaaatctga caaagcagct tcctgttctt tagctttcat ttgagcaacc tgaagacgca 59280
aagcttctag ttcttgcttt tcttgttcag tcatttcttc acctgaattg ttaacgttta 59340
aacttacagg agcctcttca gaaccttgta agtaatttaa gaaatcatct tgagacatga 59400
ttgagttaat taaaccaagt tcaagagctt cctgagcaga ataaacattc gcctcagtat 59460
tctttacagc ttcttcagag agattacgag attcggctac aaaacctgta aaggttgcgt 59520
aggtatcatt aattctcttt tgaagtcttt ctttgctttc ttctgagagt gcttggaatg 59580
gtgaacccat acctttaaac tcaccagctt tgatgacgtt aattgttacg ccattctttt 59640
caaatgcctt agttaattcc tgatgaacca taattacacc aatagaacca acatctgcat 59700
ctggtgatgc aataatttct tcagcagaag atgcaagagc gtatgcagcg gaacaagcga 59760
actcatctac ataagcaata attttctttt ggcctcttga agccataatg tgacgtgcta 59820
attcaaagca acctgaagct tcaccaccac cagaatcaat gtgcagaaca atagtcttga 59880
ttgactcatc tgctaaagct tcgtcaaagc ctctacgcaa accttcataa gagcttaatc 59940
cacctgtaca cattgcatca atgaatgtca tacgatgggt taaaccaccc ataataggga 60000
taatagcaat gtcatctttt acttttaaaa gacttcttgc ttcacctttg ggtttatcaa 60060
agtttactgc tgcttggaca tcacccagca atctgttatt cacataagtt gctgctgagt 60120
gagctaatga ttcagtggct agtaaaggtt ggttgaataa tctatcagca agtctgaaga 60180
tattcgaact catttttact caccctattt gtttaaatct acagagactg aagagataac 60240
atagatacct tcttcagcaa agaattgagg tttgctaaga gcaccagttg ccacgcattt 60300
atcgttagca tcccacaagc tatagtgtga gaccgttgca gaagctggga cagtaatatt 60360
aactgtgtct tctgaggcaa ttaagccatt atccggttca gaaaaataaa tagccactgg 60420
ctgagtaacc ttatttgctg tagggtctgc cgttgggtct acattgtgta aaataatagt 60480
cgttggggtt agcgtggcga ggattttatt cttaccatca atagttaatg ttcccattaa 60540
ttaaacctta ctttttgttt aaggactgtt gaatggttac cagattcatc taccacattt 60600
acaatcatat catatacttt acctttgaca agtactttgt aatcattttg tgaaaaaatg 60660
tattcaagtc tgtttgtttc tttattaact gtcattggag attgaaatgc agtatcgagg 60720
gtaatgaaag cggcttcaat gctattaaca ttgatacgtt tattctcgca gttatataac 60780
tttacaccca aaaggcatga actgtcaaat ggaattttaa caatttcact acaatttcct 60840
gaaataaacg gttttccact cattggtgca tcaagtaacc tgcaaatagt gaaaacgtca 60900
gagattccac catcactaac ataacctgaa aggctgaccc ttgagccagc ctctacagat 60960
aacttatcag taatgataag gacaccccta tacgaatgca ctcgtgtagc atttgaaata 61020
gagataactt cagccattat ttatttgcct tatttgctgt gcttgggtct ttcgctgaag 61080
gtgtctttgc agtaccttct ccagcggtct tataaccatc tcctgagcgg ctttggctat 61140
ttggagaaag cttttcagat actggttgag actcatcagc aggaggaaga ccaatatgct 61200
ctctaagttt gttagatagc tctttgtcaa cttccaaagc acctactgca acagtcttct 61260
gaatataaga accaattgct tcaaggtctg gagtttcgat atcatcatat gtgatttgta 61320
catgttcttc atcatcccac atattaagag cataagtctg tgcaactaaa tcacggttaa 61380
ttacgttctt aatttgcttc agcaaaatat ctactgacat tgctaataag cttgtcttag 61440
aatctgcaag agagaatgaa ccatattttg actgacccat agcaagaaca tctgacataa 61500
atgccatcat aatttgcttg gaatatctgt caatgataga acctgtatca tatgctttag 61560
caccctgtct agagactaat gagaactcaa aaatatcctc tttagtatct gggtcgatat 61620
atctaggcca gattaaacct gctctgtcat tagcaatcat atcattaaca acagttttgc 61680
agtattgtac gaaagctttc ttttcaggtt ctgcattttc atccagataa tctggtggta 61740
aaccaatctt tggcatacct accaagtctc ttgaaacacc aacagcttcg tactcttcaa 61800
tctgaacttt atacttccac ggtacatagg cattaagtaa tggtgaacga ccttctgggt 61860
ttccatattc atcatcatac ttaaacagca tgaatttagc tcgtggaagt tttcttgtta 61920
gtggtctttc tccaagatta attgctccag caatatgtga aacatttctc agattctgtc 61980
taacaccagt aacttttcta aagtcttcgt caaaatacca cttatcaagt gttgattggt 62040
ttctgattgg taatttagcc catccaatta gaccatcatc aaattttgac tggtactttc 62100
cttttttacc ctgtcgcttc ttataaactt tctcgttaac acaaaaccca taagtgcaga 62160
atgacattac agaattaata aaatctgccc aatcatgctc catgtcatcc attaaagaat 62220
taaagaagtc tgctctttca agcattttag ggtcttgctc ttttcccttt ggaggtacga 62280
atctccagtt gacttttctg acaaacatct taataatatt tacagatgct gctacagcag 62340
ggtcacgcat cattaattgg aaagttttaa tactttcagg gaaccttagt gcctgacgag 62400
gttcttcata gattcttcca ttcttaacct tcagacccaa agaacctact tcacccattc 62460
taaatggtgg taagctttct tgtgtttctg taatatctgc cattcttctc acctagctat 62520
caacgtctaa gcccctcata tgggtttcct ctcactaagt ctgtgtgagc acccattgat 62580
ggtggcttga ataatttaac ttcgttaaga ctgttgaaag catcactggt agcatccact 62640
tggtcatctt tagttttgcc atcaccacaa aagccttcaa gctcttgaaa gtaagcttcg 62700
ttccaactac ctctcaagac ttttacaagt ccagcttcag aagcagcaga aaatcccgca 62760
aagcgggtaa ctttatcttt atttgttggt ttagctcttg cacgatagcc tttctcggca 62820
agtttcctga tgagggatgt tgcgtaggat ttaccagcag cgcctgggtc ttgagggata 62880
aaaataccag ttcgcttacc gtcactttca gcagtcaaat taatttgtgt ttcgactcca 62940
gagggtctat ctctaaatct tactacatca atgatataat agcaaccgtc ttttttagat 63000
ttacccatct taacacctgc tgtccagtct ggattagggt taatctcaga tggtaaagtt 63060
gctgctaagt cccatgctct aacatcaaat acatcttctg ggagtgaatc aacaatctca 63120
caccattgtc tttgccagta gtttgaacct tctgcacgag ccttccagtt accgaaacga 63180
agtcttgcaa cgtttacagg ggtgttgttt tccaacttac cacggtactt aggttctaag 63240
aagtcaagaa ttgggttatc atcaatcgta ccagagatga aggtgtatgt ttgaggaatc 63300
tcaagaggga acatttcaag aatcttgtct ctctcccaat cagaaaccat cacaccatca 63360
ttcattacat accaacgaat acgaccacac ttttcagggt ctggataacc ttcttcatct 63420
aagaatggtt ctacccaatc ataaataaag tggtctctgt ctgggttcat ggaaatcttc 63480
atgtatgaat caccttcagc accagaacgc agacgagtct gtaggtatga aatctgtgaa 63540
gcagagaagt gtgtaccttc gtcaaagtag attgctgagt attcaatacc ctgatgacct 63600
tcagcgtgct tttcaagttc taggtaggta aacttgatag ttgccccaga agggaatgtg 63660
atagtcattt tctgctcatg aggaatccca ccaaacttac caaatagttt ctttgcagca 63720
ggccataaac caccttgtaa ctgtgttgta tttcgacgga aatatacagc attatagtta 63780
gggtcttcaa taaatcttaa agagtccatt aacaatgcgg cagtcttacc agcaccagca 63840
gcaccaccat agagaaccaa gtcagcatta gtatttaaaa agacctcttg agaacccggt 63900
tgaggggcta catagttctt atcagtcatc aatttaaaga tgaggcgaac ttggtctggt 63960
gtgtatctta ataaagtcag aatttgagtt ggaaggaatt tagaggggtc tttaccgaat 64020
gatttaataa tttcttttac ttcatcagaa agccccaact ctccagctag gactttccta 64080
acatcttcca ctcgcttctg cttaacagca tttaagtcca ttaagcgcct ccgcaaatag 64140
aattattcag agtctgtagc agtctcttct ttcttaactt cgaacttttc atcaacagca 64200
tcaaggagtg catccataga tgcttcttta atatcaatgc ctgttgctac agataatcta 64260
tctgcaattg ccatgatagt gttctgcata aattctagct ttttattagc ttcataaagt 64320
tctttgtaaa cggtctcact cattatgttt tctccaaata attaaccatg tttcccctta 64380
aaaaagacgg cacagagacc gtcaaggaga aaccacaatg tacgtcagag acatactgta 64440
taagccttct tataaagtat aactctgtaa aaaagccctg tctaaaaata ctcttcggta 64500
gcgaaaggaa gaatacttat agcagggcat tattattatt attttaattg agagagagta 64560
aaataacttg gagaatccga agggactcga acccttataa acctgttttg cagacaggca 64620
catagccatt tctgtcacgg attcaaattg gaggaagata ccagacttga actggtacac 64680
cgatttctca gctactggca gtttagcaaa ctgctccctt accttttagg gttaatcttc 64740
catttatttc tgtaatgcgt tcttcatttc tggtgttgca atagtgtcaa ttacaccagt 64800
tttacaggca tcgtcaaacc agtctggtaa gatacctgct aagaacccat tgatattcgt 64860
tttaaggtac tctgcaataa gggcagcttc ttcttcaatc atctgcacaa cttcatctgc 64920
ataagctgta atctttgaaa taccttcatt aatcttacta acagcctgat tagctaaatc 64980
agaaatagta tcaagaccct catcaatagc gtcttgtaag tcacttaata catcgttaac 65040
actctctagt gtactgttaa ttgtatcaat agctttttga ccatattctg tagcaacacc 65100
cataatacca ctgaacggtg tacaaccaac ttgttctcct gctgcaccca taacattgga 65160
ataccccttt gctacctgca tacgtgatga aaactcatca atagattttt gaccgtagtc 65220
tgtcagggtc ttagtagttg ctgtagtgct tgtaaggctt gttgtaaagc tgtttagaag 65280
gactgttgta agtccagcag caacaagttt ctcctgcatt gtagggtctg ttacagaact 65340
aatagagctt acaagtgatg tagaagcagc tacggttcca ccgagaactg ctgcaccagt 65400
aataagtggg ttagaaaacc ctttaccggt ttttaagaga ttaaaaatct ccttaccttg 65460
ttctgtcatc tctttcatta agcacctttg aaattggtag tccagtggga tttgaaccct 65520
cttctcatgt ttttcagaca cgcgctttaa ccatataagc tacttggact ataaattggg 65580
gtgacctacg ggatttgaac ccgtatagac catgttcaca gcatgggtca ttaccattta 65640
tgataaggcc acatttaagg actctcgtaa gaacccttag aagtggcagc ggcataagga 65700
tttgaacctt aataggacag cttcagagac tgttgcattg ccagttatgc tataccgcta 65760
aatttggtac tccatatcgg attcgaaccg atacataaca cagattttaa gtctggcctc 65820
tctgccaatt ggagtaatgg agcattggcg ggggatgttg gaattgaacc aacttcttcg 65880
atttcaaaga ccgaggtttt aaccttgtaa actaatcccc tttaaatctt tactttctta 65940
gtggatgaat aaagaatgcc aacataactc ttccccttga gaatgctctt tcttctggga 66000
caacactttt tagtttccat ccaatataaa ttctccagta aaactgttta ccaaagactt 66060
taattgatgg gataaaagcg aataatcccc aagcattact gttccacatt aggagataac 66120
ctgtctgatt atcttcaggg tcagaactta cgttgatatt acctttccac ttagtaacat 66180
cttttacatc tcttcctaat acatggtaag agaagttata agctttgttt ctccagagcc 66240
atccgactct ctgcatgtag acacccagct taccaatctt tctaatctta gcccatcgtt 66300
cgatatgacc ttcgtcacca tcaattgggt tgtcatatgt ctccatccat ctaaatccga 66360
aaggtaagtg gcctttcttc tcactgtaga atggaactac aaaaggtgct aagataactg 66420
ctaagatttc tgcaaatggc tctaacaaag ctaagaaaat ccatgaagca tactttaagt 66480
atctcatcta aatagcctct ttaaattggc gcaggataag ggattcgaac ccctattaac 66540
agcttcgtag actgttgctc tatccatttg aactaatcct gcaaaattgg tgttccaaga 66600
cggattcgaa ccgtcactag tacaaggttt gagcttgcat cctctaccaa ttgggatact 66660
ggaacatggt actcactaaa ggacttgaac ctttttcttc atcttgtaag ggtgatgttt 66720
taccatataa actaagcgag tgtgaaagag gtctgaagtt gtgccgctaa ctcaactccg 66780
tggaatttgt tacggtcttc agttgactca aagcggtctt tcacttgacc tctgaattgg 66840
tgctgctcac aggacttgaa cccgcatctc catccttaca agggatgtgt aatagccaat 66900
tatacgaaag cagcattaat ttggagcatc cagagggaat cgaaccctca acctcagttt 66960
ggaagactgt aattttcccg tttaaactat ggatgcacta attggtggag tcactgagaa 67020
ttgaactcag ttcccaagtt tgcaaaactc gaattttaac catataaact atgaccccaa 67080
tgtctggtac aggtggaggg aattgaaccc atcgtcttac tgattaagag tcagccgcat 67140
aaccattttg ctacacctgc attaatttgg taggagacaa gggattcgaa ccctcaaaca 67200
ccaacttcta aggatggtag gtttaccaat taccgtcaat ctcccattaa atctttttag 67260
agaacttcta agaacctaag caacaaggtt ccacaacaag ccgtaataac cttagaagcc 67320
ctctaaaaag acctaaaagg tctttgcata gtcttgttta atccggtgac tagaaccttt 67380
ggagagcttg atattatctc ttctccgtga cacctactag gtgttttatc agtgtgtacg 67440
acaatacacg agggatacca aagcaacatc cgaatgaagc ttggctttaa aaatcttatt 67500
aggcggtaag taaaggagtc gaaccctcac cgtatctcta cagtggcaac agttttcaag 67560
actgtttggc tgccattagc cgctacctac ccctaataag actttggcat gggacggagg 67620
agttgaaccc ctttgaaacg gttttggagg ccgttgctca tgccttagag tcttaacatc 67680
gtcccatact ataattcgaa gcttaccgta ttatctcaac actgtaaagc cccttctcag 67740
attatttaca tgttctggaa aacatgagat acagaccacc tccctacagg aagacccgct 67800
taggtcgtct aagtaatctg ctaactaaat tggtcgaggc ggcaggactc gaacccgcat 67860
actccactta ctcggttaac ggcggtttag aagaccgctg agatacgccc cgtaattatc 67920
tttaaagact ctcttagaaa gcccttaaag atgcccacct tattaatcat accgtgggcg 67980
agtacgccaa attctttgat gagggattgg aagacctcac tggtgtttag cctatcaagc 68040
tactgccaga aaaacattgt cgtttgcatt tattttaaat ttgcaaaata gacgctacgc 68100
aacgaaaact aaggttacta taaagtagtc acatctcaat gtcaacaact ttattgaaat 68160
tggttgtagg tgagggtatc gaaccctctt ctaacggtta tcagccgttt gcattaccat 68220
ttatgctaac ccacaattgg aggttcagat gggaatcgaa cccatattca tagggcttat 68280
gaaacccctg cattacctta tctgcgactg aaccatattg gtagaagtgg agggattcga 68340
acccatcgcc tgtcagatta aaagtctgcc gcatcaccat tctgctacac ttccattaaa 68400
ttatctttgt agataagcta cccagacttg cttaaggtct gtagcgatgt gcctttttgg 68460
gtttaaatct agaatttacc ctttcaccct ttgtggtcgc atactcacag gtaagcttaa 68520
taacttatct acaaagataa ttaccagacc gttgtttatc atcttaagtg ctaccattac 68580
accaactcga cctctgccga gtgaaggaat cgaaccttca ccttttcttt accggagaaa 68640
tagattgttt aagttttgct gtaaacggtc ttctgtaaat ttgttgagcc agaccaagtt 68700
ttaaattttt caaaattaaa ttggcttgaa aatatcatac gtacttgctg tatttggtct 68760
tccgctcctt taaaggatac tcgtaagaac cctttaaggg aggggctaaa tagccctctc 68820
aaaattggcg gttacgaagg gatttgaacc ctcatcatct cccgtgacag gggagtattt 68880
taaccagatt aaactacata acctttattg gtgtgccgtg taggagtcga acctaccgag 68940
tctcaatgac aagggattta cagtccccac cgctaccatc tacgggataa cgacacattt 69000
aatttctatt caggctaagg acttgcacct tacaaatcgg tgagcaatcg cttctctcct 69060
aaatttggcg gttagtcagg gattcgaacc ctgtgccatt cgcttaacag gcgaccgcac 69120
ataccttatg tgcttcctaa ccatgtttag tattaccaga ccgtatcttt caatttttcc 69180
gctaaaaatt taataaatat tgctgtaaac ggtcttctgt aaatttggag gcgggtgcag 69240
gagtcgaacc tgccgatacc atgctaatga gacatgtgag acgccctttc tctatacccg 69300
caattcttaa tgaccagacc aaatctcttc tttgtccgat ttaccttcag atgaataaga 69360
ttaagtttgc tgtaattggt cttctgtcaa aactggcgtt ccagaaggga tttgaaccct 69420
cagatatcca ctttgaaaga gtggtgactt taccattttg tctactggaa cattaatttg 69480
gtctctgttg gaggacttga acctccggcc ttaccgcccc aaacggaacg ctctaccaag 69540
ctgagctaaa cagagataaa cttttcaaac tctatgtaac cactttaaca ttatttttta 69600
gtggttgtca agaacttttt taaaatattt ttcagtatct tagaaaagct ctcatttcgt 69660
ttctatgtag aacatattaa agggtattaa acacattgtc aatacccttt ttaaaacttt 69720
tttaccagat ataacggtca atcattactg ctttgagcat tacgctgatt gggtcaaact 69780
tctcaccacc aagcagagct tttaaagtgg ctggagagaa accagatacc attgctacac 69840
cgttatcctt aacggacact tcgcaagtac cattacggtt tgctaagtac cagaatacca 69900
gttgtggcat ctcatatcca gcttttttgt acttgctctg aattgcttca aagtttgtac 69960
gaccgttagc tccatcaacc tgattgaact ccatatcaga gaatatgata agcttacttg 70020
gcatatcttt ctgagtcaag ttgtttcttt tacctgtctc aagaatacgg tcaaaagctg 70080
cttgtaagtt ggttgagcca tattcaacgt gtcgcatcac ctgacgatga cggtttcgta 70140
aatcaccact cagttcaatg aaatgagggt ttgttgaata aaccatcaac tcatccttaa 70200
agcaacctgt attgcgttct gctacataca aggcaagtga tacaccaata tcaagtgcag 70260
tgattgaacc aaaattcacc caagacattg agcttgaaac atcagtcata cataggatgt 70320
tttcaccttc tgccatccag tttggtagtg ctttccactg ctcattagca acatctgcat 70380
taccatactt aacagattta atcacatcgt atgggtaaac agcaccagca ttaatcttag 70440
cctcgccctt tgataaggac tcaatgtaag ctttgtagcg ctctccatct ttacggttaa 70500
acagtttttg gtaacgtgca gcagcaagtg aaggaatctt gctgtagtca atcttaccaa 70560
actcattagc agagattttt tgctcaactg tatcagatag tgcagacaac agtgtgcggt 70620
actctttctc actcaagttg gcaaacttgc agaaacgttt tacaaactgc ttatgacgtg 70680
gtttgactcg tggtaaccac ttagccgcca aacctgctgt tgcagggtct agtaatgctg 70740
cttctaaatg tttgaaggca tctgtctcga aacgagtacc tacgaagatt ttgaagtcat 70800
caaaacgacc aagttctgca atcttatcca taatgcgaag aacctgcgta gactctaaaa 70860
ctttgtcttc aattgcttga agcaaaacag ttctgaaggc tttacgctca cccatacctt 70920
ctcgtacatc tcgcatatga agcaaaatac gaactgcaac atcaacatcc tcacgcaaag 70980
ctttgtagaa caggtctggt aagatttcta cattgctacg gcttgaacca gcagctttgt 71040
aaaagtctac aagagcagac attgatgaag tatggtttac agcaccattt tcagttcgac 71100
ctgcatgaag gtgcgcatgt ttaaataatt cgctcatatt ttactctctt ctctcatttg 71160
ttgtttgatg tgacagactt tagagcaact ttatagagtc tgtcaacatc ttttaaaatt 71220
attttttaag ggttgcaaca gcagagaacg ttggtttgtt gtctgtctga gactgaccac 71280
cattatcagg agtcttctta gtgtctgctg caattgcttt catttcacct gctgagtggg 71340
tcatgatagt tttaccgacc tcaatcatgc ttttgatggt tacgtcgtct gtgtttaacc 71400
caaactctgc aagcttagca gcgtcccttg taacaatcgc ttcaaacaac tttgcagcaa 71460
actcagcaga attatcaatg gtcagttgag cttttacgag tgaacttttg ttacgagagc 71520
cttttggtct tccagatggg tttccagact ggcctttttt aaactggcct ttgtttgttc 71580
tgtttttcat tggtatgcct cttataagac ctcttaaaag gcttttaaga taaaagatag 71640
atatccagaa agatatctgt ttaaatagcc tttttagagg aaccttttaa gttatcttct 71700
aagtatttat aagcctacac cttgtcaagt actttgtcaa caactttttt aacttgcaat 71760
agttcttgac ttgttgtatg gattactgta ccatcttact taaagctgta ggtctgcctt 71820
gttctacaaa ggagacttaa tgagaaaatc aaataaaccg aagaaaggta agaataccaa 71880
tcactgtaag gaatcaaaaa gggtagagtt aatctactat tcatcctctg aaattggtct 71940
gtacctgttc tttcaaaatt acagaagaca agaggattat ctatgtgtag ttcccaatta 72000
gaaatcgcag atattataga tttatatcaa actgcaaaga gtcatggcta tataacctca 72060
attggaaaga atagtcacta tgatgctttg actggaatgt atttcagggc aatggctcaa 72120
tctagtgaac aacacttagt ggtttcttcc agtgagttta cttcgtttct ctattgcagc 72180
aaaattataa atcgcaggag aactgaaaaa tgttaacagt aagttttaat tacaatagtg 72240
acggctctgt atcaattaac tcaccatatg caaatgacct acttaaagag ctagtcaatc 72300
agtgtgagag gggtcttcac tatgttcaaa attcttttaa acagaagact attgctaata 72360
acttgatgcg tgtaacagtg acgtcatcga atccaaatta tgactttgat agtgaaagtc 72420
cgtactcatt agttgcgctt ggagagagca gccaattcaa acttgtttgc cacgactcag 72480
aaacattcct taaggtattt tccaacctta tccataataa taagtacagc tacgttgatg 72540
gtagtgtaaa tttctatcca gcaaactata catgtttgtt gattgataat atgagaaaaa 72600
gtaagcaaga gcctacagaa atttcatttg atgtgaactc tagtccagac gcagaaacaa 72660
gtaagaactt tgatatgagt tacgcattgt cactcagtaa gaaatccgag ttcattgatt 72720
atgtcaatgg atttggtttt aagtttgatg agagtatgaa tcttaaaaaa cttaagaacc 72780
tacttaagac caaagcttaa gtgtgaatag gggctgatgc ccctttaact atttataagg 72840
atatttaatg aaagctaaga gtgcaaaaga cttttattgc ttcctacaat cctatatcaa 72900
ttccgtgcaa aatggtgaaa ggtacaacct taatgatgtt atcccaacac ctttgacatg 72960
gagactcagt aaatggcccg aagaagatat tacaccaaca agcgaacaac gcacctacaa 73020
accagaaatc aatattccag actctgagaa tctattatat ccaatgttcc atattgttgg 73080
tcttggaaga ttccttatgg atattcagta tgtaattggt aaaggttata aggttaaagg 73140
tattgttgtt ggtgatgtat ctccgaatca caaaggttat tttagactaa acgcacattt 73200
agaggctgaa aagaaatgat taaagcaaag acttacccag acttcaaaga gtttgtgaag 73260
ggcttcgttg caaacgtaaa ggctggtaag aggtatgatt ttagaacata tcaagaggct 73320
attttaccac ttacctatag ctcgtattgg cctgaagctg atattgcaga agttgagaag 73380
tttgactata aaccagacta taaagttcct tttagtgatg aactccttta cagtgttggt 73440
gcacagatga gaactgctga tttcttcatg gatttgcagt atgcaatcat caatggtaaa 73500
gatgttgaca ctatttactg tgaatggttg gcaagagtta atccgttctc aatgttgaat 73560
gctaagctga aggatgctat caagccacca gcaattactc agcaaccaac aggtcaaaca 73620
gtcaatgagg gtgacacact tactctaagc attctagcaa ctaacgccac tggatatcag 73680
tggaagaagg atggtgagga tatccctagt gccaattctg caacttacac aaaacagtca 73740
gtagcacctt ctgatgctgg tttatacact tgtgttgtat ctggagaggc tgggacaagt 73800
gttacctcag atgcggcaac agttactgtt aacgcactac ctgtgattac acagcaacct 73860
tctagtcaga ccatcaatga gggtggaaac atcagcttgg aagtgactgc aacgggtgca 73920
acgggttacc agtggaagaa agatggctct gacattcctt cagcgacaaa tgctacctat 73980
agcaagtctg gtgctctgcc aacagatgct ggttcataca cttgcgttgt aacaggtgca 74040
ggtggctctg tcacatctaa cgcagccaca gttactgtaa atgcacttcc agtaatcact 74100
caacagccaa ccaatcaaga aatcactgaa ggtgagacct tgacgttgaa tgttgtggca 74160
acaggtgcga caggttacca atggaagaaa ggtgaggaag acatcctcga tgcaactact 74220
gcaacttaca ccaaagaagg tgcaaccact gctgatgcag gaagttacac ctgtgtagtt 74280
actggtgcag gtggctctgt aacatccaat gcagcaacag ttacagttaa cccagcaggg 74340
gaggcataat gcaactctca agaaaaggtt tagaagctat taagttcttt gaaggtctta 74400
agctagaggc ttacgaagat tctgctggaa tccctacaat tggatatggg actatccgta 74460
ttgctggtaa gcctgttaag atgggaatga aaatcaccgc tgaacaagct gaacagtatc 74520
ttctcgcgga tgttgaaaag ttcgttgcag cagtgaacaa agctatcaag gttccaactt 74580
ctcagaatga gttcgatgca cttgtaagtg aaacatacaa catcggtatc acagctatgc 74640
aggactcaac atttatcaag cgccacaacg atggtaataa ggtaggttgt gcagaagcta 74700
tgcagtggtg gaacaaagtt acagtcaaag gtaagaaggt cacttcaaac ggcctgaaaa 74760
acagacgtag gatggaagct gacatttatc ttgacagtgt atatccaaag taatatcttc 74820
ataggctcct tcgggagcct tttttatttt ctaaggagaa agctatgaaa ctttgggcta 74880
gtgactttgg aacttttaag tacactcgta atggctcgct tgtacgcatt gtcgggaaca 74940
atgtggtttc aagaggtgac aaggtatata cacggtttac tgtagagctt gttgagttgt 75000
cacctattga gtctgtgaac aatggcctgt tcaagtttga gacttacaat gtcaacgaac 75060
atggacaatt caaccctctt ggtgaaagtg gacttgatat tatttcagag cacccattga 75120
caaaagagca acttgcaggt tactataaat ctgttctgga aaggcagtta gcaacacatg 75180
aacaagaagc taaatatcat ttacaacatt gcgaagcttt aagagcaaaa atagaacaag 75240
cagagagagg tttttatgaa taaagagatt caagtaaaag ttgacacttt tggccatgat 75300
ggtcgagaag atgctggact tgccgacgca gtgatttttg agtgtaatgg aaatattagt 75360
cttcgcttta caggctcagg tagcacactt cgcagaatgc gagatgatgc agatggttgc 75420
cctgtatttg gctggtatga tatggagttc cctctttacg ccatccatta tccagatggt 75480
ggagaagact ggacaactca atcaatccgc gatgagctta atggcattcc agttgagcaa 75540
gaagagcaag aacctgttga actcacattc acttttatca aagaagagaa agtaggtgac 75600
ttgtcagtaa ctgaagcaat tcaagtctca caggttattc gttaatgagt aaagtacagg 75660
ttgttttccc tatttgtgac ttctcactag agcgtgaact tgacctgtac gaagaaatta 75720
ctgacgaaat tatctggtct gttgtagaag aggctatcaa gaagctgtat ggtggcctct 75780
taaatacatc aagtaagaag ttaagcacta agcaagtagc tgacccatat atttcatacg 75840
atgcctacaa caaacctttt gaaaatacct gctttgacct tatggtaggt aataacaaag 75900
tcaactattt cttcgtcaga gagtttaacg atgagtaagc tccatgtcat agtgtacaaa 75960
aacttctcag atattaaaga gtctttaaca aataagcttg acttacaacg taaaagactc 76020
tttttaatgt acgatataga caactacaac catcctaaag agtttaacta caaagatggt 76080
acaaaggttg ttgagtttga agattctgtg acggtatatg tcaagcatga cttaccagca 76140
aagtacatag ggatgctaga gtactacata ttcaaacata ctggtatgcg tggtgagtct 76200
gttaagatat cttctataga agtttttgag aaacctaaca cacaacttaa aaagtattta 76260
atgaggaaac tgtaatgtca gaagaacatg agcatatcca accagaagtt acattagtaa 76320
aacatttcgg aaatgtagaa ggctgtgtag cactttttca gactaacatc aattctccag 76380
tcaaactgtg caaattgacg ataaacacca acaatatcag tgtatgtgtg aatgaagaag 76440
tccaatactt caaattcaat gataaagagg ttgatgctgc actgctgaaa tacagagcaa 76500
atcttgaaaa agatattgac cataaagaag ttgtgagtct ctttgcagac ctccataagc 76560
ttcttgataa ggttgaaaaa agaacatatt acatgaataa tggctctatc atcaccacta 76620
tgatttcacc atgtatctct gagccaatct tcacagatga tggtgcttat tagcagtgta 76680
gcatcagcag tgtctgattg gtggttaaaa aataacgccc tgaaaacagt gattgatgct 76740
atcagagaac acatccctac atttagtccg tggaagggta aaggtgatga atttatcgca 76800
ctgttgagtg aagagagtaa caagcgcagt gcattactgc ctaaaaaata ctcttgaccg 76860
agtatacaat attgattatg atgggagcta tcgagaggta gttccctttt tagtttctgg 76920
agaatagata tggctaaagt gaaagaacac gataaaatta ttttgtatgt taaacagaat 76980
ccttcaaaga gtgttgaggc tgttgtaaca cacatctcac acaatgggat agtttatttt 77040
cgaccactga agaacttaga ctttgacata acaccaaacc acacatttag tacctccgca 77100
agactgtgtg caggaactgt atactcaact gctgagattg aatctttagc tttcacaggt 77160
gcttacaaga actttatagg gattgcttta aaggactctg aggaatcttt taaatttttc 77220
atggatgctg ctaatggtgg tagtagatat accatgccag tctttaagaa agttgataca 77280
gagattgtca acaagtgcga agaaattagc agtaacctga aggaacttaa aacatctgtg 77340
cagaatgcaa tttacattat tgaaaagaat cacttagaag ttcttttcag agatatgctg 77400
aaagcagggt ttactccaga cgaaattaca agagaaactc aacgtcagtt cagaaatgct 77460
atggtgaaag aatgaaaaaa ctaactacag ttgaagatta ctacaagctg tctctattag 77520
aacaatatcg tagaagtcag agtataagaa gatgctatgg cagacacgct gaaggtgatt 77580
tttaccattg ctatgacgca gatttaaaag gtgtaacacc tagaggtaaa gtcctacaaa 77640
gacttgttga ccttgagtgg aacaaacgat tgagagggat tggaaaatga tttacgaaga 77700
aagatacaag atagattacc agaagactgg tcatcatgtc tccctaagag taacaaaacc 77760
aaatggagat accggtatca tagcacattt tggtggtgat tattggtatg gtacaggttg 77820
ctttgaaggc tacactcaag aatacttgaa agcattctac agagacttta caaacgacta 77880
caacagagtt gttgacgaaa agaataagtg cattaagcat gaacaccatg ccagaggttg 77940
tttgagtaca gtaatggtac tggcattttt cttggcaaca ttactggcag tatcagcaat 78000
cagttccata gctcaagatt tgaccattac acagattact gcaaaggtat atgatgtttg 78060
gtacttgtat gctgtccctt tagttggtat catcatctca ctaatgagat tcagagttca 78120
taagaaacga cttaaggact ctgaagttaa acttgaagag gtaagtaaag aatgcaacct 78180
acaattatag ctgtatgtgt tcgttttgca atcgctgaaa tgattaacaa ggcaatcttg 78240
aaagatgctt atggagagac taagtaatgg ttaagacacc tgtaccaatt tttggatttc 78300
cttctataga agagtttaaa gtttatcttg acaaaaactt ctacaatgag cagcctgtta 78360
ctttactgaa gagtgactta tcagagcttc ttgatatggt tattaaggca acttctgaga 78420
aggagcctga gcagaaagct gagaagaaga ctagtaagaa atccgacaag aagactgaaa 78480
agtctgagta ataacttgag gggttacttg atagcccctt tgtagaaact tagaaggtag 78540
cgaatatgca aatcatagct ggtcaagaac ttgacatcat agatgcaaaa acacaggagt 78600
acatcgcaac agtgaaggtt ataggagtca gggagtggaa tactgagtac ccaattcaat 78660
gccttgtgct ggagaagttt aaagctaatg gtgtacactt ttaccacggt gactacatca 78720
gctttaacaa agatggttac tggcgtggta gtgaccaccc tcaagcaaat gagtttaata 78780
tgcgtctagc aataccacaa aaagataacc aacaaaacgt aaaagatatc cttgtagaag 78840
cctatgaaga aggtataata gatgttgtgc atgatgttga aaaagcttta aagcttgtta 78900
tgccacattt agagtctgga aaactgactt tagagatgct taatagggtt attcggagag 78960
cttatgaaag taaaagagat agtgcaaaga gcaatgcttg acaactcgac taaagatgag 79020
atgtacacag aaatttgtga taagttaaac tgttcaagac atgctgctaa ggttcttgta 79080
cactgtttta tctgggaatg ctcagaagct tatatgaaac acgtagcttt tgagagttct 79140
catttactcg gtgatgcaaa aataggtgta gcactaaaag agcctgagat gaaaacagta 79200
cctaaagttg gtaatgtgta taaactaaaa aattttatta caggcgagat tgttgcagaa 79260
ggtgaagtgc aacaggttta tcatgatggt aaatatctgt ttaagatatt cgagtatgat 79320
agtcgttaca gacacttatg tggtattaca tttttagttt cagaggatga cctcatcaag 79380
aacaatagta ataaattcgc agtaccagaa tatcaagttt tacgataatg gtgtgataga 79440
tatggaaaaa gataacttaa aagaagttga aggtaactac ctagtacttg actggagtga 79500
tgttcgagaa gcactctccg aagaaaatct tgactggttc gaacaaatta tctttgaagt 79560
acggcataac agagaagttg aaaatggtaa agagccactt gaaggaatct ttgtagaaag 79620
gtcttatccg ttctatgaag acactttgca gaaagttaaa atgtacttta aacagaagaa 79680
ccgtaaagtg gtcactatgg tatctcttgg tggacagagt atgtctatta tggaagatat 79740
caaccctaag agaccgagta aaggtgtctg tataaaaatc acaggtcatg aagagtatat 79800
ctcagtggat gattttagtg tattctgtaa tggttctgct gtctttaatg gatttcacta 79860
tgacatccga gtgtacccaa tcaagggaga tgctgttatg atgcagatat tcgataacaa 79920
caatggattc actcattttt atcagacaac taagtcatca ttgaagacag ttttagaaag 79980
cctgatttaa aatatcttaa gagcctcctt aatcgggggc tttttaattt ttataaaatt 80040
ttatcggttg tcccaacctc tgggtcacca tcatatgtga ataaaatatc tatccttctt 80100
aacaaccttc ttaatcttac tctctatata tagttatata gtactatata gtatcttagt 80160
aactatctag tggtcttaat agccctctgt atagcccttc taaatagcca tctaaacact 80220
cttctaacaa gccttcttaa caacctatac aatcacctta aacatctctc tacaccttct 80280
tagaagctcc tatacacctc ttaatctcta tgttacctat gacactctct ataggtgttc 80340
actaggaaat actaagcctc tatatagtct gttttaacaa acttaacagg aatatcttta 80400
cagaccttat ctcacaaggt ctaacacggt actaacaagg tatcttataa gcctgtttct 80460
acctgtattt ttaaaagact gtgtaggtaa ttattcagaa ttttaaaatt tttataaaat 80520
aagactgtca agtcattttt tagggtcttt gaaggaactc aaaaatatct gattaggatg 80580
tatatctgct tggtagccct taacagctcc cttacaagta ctttgcaaaa ttttaaaata 80640
tccttaacaa tctcttaaca gttctcacat cttcttaaca actcttcaaa ggtcttaaca 80700
gattcttaac agaactaaca aggctgtaac agtttcttaa cagttgatac ataggtttta 80760
cagaatctta acagatgact aacaagagct aacaagttaa ttttaaagag ttattttgtt 80820
aagataatta acaagtttct aacaagtgtt taatcttttt aactagctat taacatggtc 80880
actatctctt tcttatcgtt ccctttcctt tcttagtgat tccttaaaca tacctcttaa 80940
aagctctcta tagctcttta taagctcttt taagtctctt cctagtagtt acaccccaac 81000
tgtacagata actctttata gggcttcaca ggagctttta aggctatgtc aaagactctt 81060
acaaggcttt tacgtggtct taacagagat ttaacaaggg gctaacaagt tgctaacaag 81120
tgtcttaaaa gcataactat gtagggattg ttaagaaatt gttaaggggg ctagcattta 81180
cgtaactttt aagctacttc aaagtaactt tatagcccct ataacatact tttaagacaa 81240
taacaagccc tttttagtag ttcacaagat aactaacgag atgataaagc ttacaccctt 81300
ataagattct tttaagcctc tataaagccc tgtaaagagc tttttaagat aggttaatgc 81360
aatcccttaa gttaggttga taaggctgtt atagagcttt atagagcgtt ttaggggttg 81420
cccttccctt tcgggttatg ggcttgtatt taagttgatt tgataaaagg ctatagaata 81480
gactatagag ttattgcttt taaggtatga gatagattct atcgaatcag tggatagatt 81540
ctagaaaggt gagggaaagg gattgtataa ggattcgata gacgtaaaaa agccccttta 81600
aataggggct gttcaggtat ctggggctaa atagccccgc tgcgtcgatt agttataatc 81660
ttgtttgttg tagtacaggg agacaatttt ttctccccaa tagtgataag tataaccatc 81720
cttgagaact tcttgttttg tttcctccca gaactcatcc gaaggattat aatctttgat 81780
ggtctgactg ataagacctt tcaccatttc aaaggcgtta tcagtgcctg caatgtcaaa 81840
tttaacatcc agagaggaaa aaccgaactc gttaacagta gcgcggatag ttgccatttt 81900
tagattctcc agtttaggaa gtagtttaag gactttattt agtggggaag gtcattccct 81960
ccccgttgat tagtaattta ctgcatattg gcgtatgttg caagcctttt ttcgttgtat 82020
tttgtaagaa tctcacaaga caaaacatca tactttgtaa cagcgttgcc tttgtcgagg 82080
tcaaagaatc caaacgcttt accatctttg aatccgacaa cttcataaga ttctttgatg 82140
atttcaccat tgaacaaatc gctttcgata cggtgaaata atgctacatc ttctttgtcg 82200
gttgcaatct gcaatagtgt ggttaattct acgttttcca tcttgataac tccagtttag 82260
gactttattt agtggggaag gtcattccct ccccgttgat tgataattta cagacttttg 82320
attgtctcgt caatatcttt ttcaatatct tttaaagttt tttctaagtt cttgatattg 82380
aaggttaaac cttcaccgag agttttagcg taaccgttag gacgtaagtc ataaaggcca 82440
atttgttcta cattggagag gttgaccatt tcaccgctta catcataagc tattgataca 82500
gtattttcag gggctttata gaacttgttt aaggacttga tagaacgttc ataagccatt 82560
ttataaccgt ttaaaaggtt tttaacgtgg ttgtgaacgt ttttacactc atctaagtaa 82620
atctctaaga ataacggtaa taattccgct acagcttgca taaagtgaga gactacaacg 82680
gttgcaacat tacgacgatg tacataataa gccccgtttt ctgacttagt gatggttgca 82740
aagtgtaccc cattacaacg aaattcgtat gtatctttta caggcttaga cattacagca 82800
tttgaacgga tgaagttgat agctttaaac ttgttcattt tgactctcct agtttaagga 82860
ttttatttag tggtgaggtc atccctcccg ttgattagta atttacttgt tttgatagct 82920
gattgcaagt aattttttga gcttatctat aaagagtctt ttaagtgaat cccttttaga 82980
taaaccccct cgaacaaagt taccaatgtc aaggggtatt gtcaacactt ttttaaaagt 83040
tttttgtaag ttattgatta actgatgaat attttttcag gttttacata gctgatacgc 83100
tgacttttta gcaggtcatc ccagattaac gcggcgatat gggcataacc ggattcacgt 83160
aaaagcttca ttgttttact tctcgtttca aaacggcttg catagtactc actattcagt 83220
gtcgttttat tcccttcgtt gtctacatgg ataatctgca tcacttgaaa ggggcttgtg 83280
tcctcgcctt tattccactg ttcaaagtat tttaggcact gcataggtga catcttatca 83340
cttacttgag ttgaccaaga tgcgccagct ttatcggtaa ttacggttac ataagccatt 83400
tttgaatctc ctagtttaag gactttattt agtgggggag gtcatttcct ccccgttgat 83460
tgacaattta cagactttta tcaggcttgt ctagtctttt tcaatgataa tctcatactg 83520
accatcaaca ctatcgaaat agattccgtc gtcatcttct gtcttaaagt aacactgaat 83580
atcatcactt gcgggatagt cttgccttgc tatctggcaa acttgaatag cattttcttt 83640
acttcctgtg aactcctcaa gccctttaaa atcacatgaa tggccattac aaatagaaag 83700
gataaggtaa acggttgtaa gcatgattta agccccctaa aagcccctac aaggggctga 83760
ttagtgttta taaggtgatt gcataggttt ttagaaaaag ctctttaaat cctcttctgc 83820
aatgtttcct gtcagtgtat ccatgatgtc acaaaaaacc tgaatactgt ccgactgttg 83880
aacactttta gcaagaagct ccagagactc atcaagatag tttaaatttg ggttatccaa 83940
tgaaggaaga caaaaaggtg aatacatagc gttaaactct gttgtataga cctttttaat 84000
ccaatttaca tcaaagtact ctgtaaaact tgctacactc gacatattat cacaagaata 84060
atcatcgttt agctcatcct ctggaatatc gagttctaag ataacaatat tttgagtctt 84120
catctgaaaa gctgcggtga tagtggcatt acctagagct tgctgaatgc cctctgatgc 84180
tatttcttcc tcatcgtcgc catagatttt attttctgga tacacgtaaa aattgccgtc 84240
catatccgaa caattccatg cgcctgatgg tttatcaccg ttattaatca ggttgaggaa 84300
attttcttga gtagtaccgt gaaaacattt catttttgaa tctccagttt aggattgagt 84360
agtaaagttc tttatctggg gcttaagata tcaactttta agccccttgt aaagtacttt 84420
atttgttatt tttgacaact tttaaaactg caagataacc taagcaataa gaatcaccga 84480
agagagtcct acccatgaaa gaatcccctt taatatcctt tgcagtgaca gttttttcta 84540
cgccgtgaaa aagaactgta tcaccggctt taatgtcttt gatgtgtaca gtctcgatag 84600
atacgccgtt aataatagcc attttagaat ctccagttta ggatttagtg gtaaagttct 84660
ttatctgggg cttaagatat caacttttaa gccccttgta aagtacttta ttgcgtcttc 84720
ttcgtaaata ttagttttac agcgattgtt ggaagctctt cgatatttgt aaatagcgtt 84780
actttttgag tagtaacctt gtaaccgttt tttgcagcgt aacccgtaaa gcggttaact 84840
gtgtagctat atgtttttgg tgtattgtct ggcatatggt acaggaaagg tttgcctaaa 84900
ttttctggtt gcgtcaacag tcggcaagta tcagggatat ttaaaaaatt catcattttg 84960
aatctcctag tttaaggact ttatttagtg gggaaggtta atacctcccc gttgatgtgt 85020
aatttaatct atctaaaaat aagtgtcaac aattttttag agttctgcat aaattgcatc 85080
gcagatatta cgaaaatcgt gatttgacaa attacgctgc aaccaagtgg cgaaaaagtc 85140
agcgattgca aagttagcat catactcgac agataaccac actttaacag caaatcggaa 85200
cattttcaaa tctaactctt tattcattac cttagatttg cctaacatgt ctgacatttc 85260
atattgcgcg ccgtctagcg tgatacctga tgcaacgtca gccaatgaac tttttacatt 85320
ttcaacgtct aagataacac tttgaatctt cccgataatg ttgtgcaatg tttcatcagg 85380
cgctgatgat gtcagaacat taatcctgtt gacggtcaaa cgtgctttta aaattgataa 85440
ttcacggtta ttaatagcca tttttaagtt ctccaattta ggattgagta gtaaagctct 85500
ttatctgggg cttactttat cgaactataa gccccttgta aagtacttta cattaaatgc 85560
agttttttgc cttttactat aatgtaactc gatgcgctat tgctttgcac atcttgataa 85620
attctgcgca aacgtccttc gtagtatact ttccaactgg ttggaatctt tttcccgtat 85680
cctgtagcag ttttttggag tcctgcaact tgccaatcca aaatgtgctg ttcagcttgt 85740
aaatcctcga agtgcattac accgttcaca tagtgacgta gtgtgacatt taaagtacga 85800
ccgttgttag tagtatacat tttaaaagct cctagtttaa ggattttatt ttgacagttc 85860
actaattaag acatcaaaga ttatatcatt atctgctaca gttgcaacac cagtgatata 85920
atcaatttct aagataacaa caactttcgt acataaaccg tcatctatat cattccagac 85980
aactttatca cctgcacaaa acattttttg aatctccagt ttaggacttt atttagtggg 86040
gaaggtcatt ccctccccgt tgatgtgtaa tttaatctat ctaaaaacca gtgtcaacag 86100
ttttgttttc gtttgctcaa ctcttgaaca attacagcgt agtagtcttt taagtcactg 86160
acatttaaag catttatcag agaattataa tcatcgacta actgaaaatc atctttgaag 86220
atgtcactta tctgataggt tttcgcttta tatctgaagc tcatatctag ccgctttatt 86280
tgctcatcac tgagacgccc tagaaagctc tctacaaggt tattgctaac ctgctgtgtg 86340
attgccttat ttgctagatt ataggcttta tatgccttat atagcgcgaa aatggcaatg 86400
attgcagtga taccagtcat taacatggtg tttcctcttt acgtgatact ttgccactta 86460
cagttatctc ataggattct aataaatcgc ttttagactt tactggactt ttaaaagtgt 86520
catcatagat attgacttgc aatacaggtt ttttatactc cctgcaactg ttccataagc 86580
aaatcactac acgacgacct tttattttgc cgtgtaacca ttccataaca tcatggctat 86640
tccttgatgt ttcaatgaaa tcaattttgt tgagtaattt gctcagattt tcacgcactt 86700
gatttgataa tttcatttta gaatctccta gtttaagggc tttatttgat aaatctctct 86760
atctggggct tactttatcg aactataagc cccttgtaaa gtactttatt tgttaatttt 86820
gaagatagtt acgtaaccat cactaccgat tatttgaggc gctccagctt tttctacttg 86880
atagcccata ttttcaagat gataaacagc atcatcataa ccatatgcgc caccctgata 86940
acgtctctta tcacatttta tcaggttttt accacgtcca gcaagtgcat tcagtacagc 87000
tttttcactt gatagcgtac catctaacat ggttgccgtg taatggttga ttctattccc 87060
gtttacatca tacttaaaat tgaatgcgtg aacaacgata atgttgcctt taaaattttc 87120
atcaagtgac ttctccagtg cgttgcgaaa gttgtctttg ttgatgaatt taggtgcacg 87180
atatgccatt tttgaatctc cagtttagga ctttatttag tggggaaggt cattccctcc 87240
ccgttgatgc aaatactaga taaccacgtt caaagagtca agaaaaaatt ttaaaaatat 87300
ttttggcaaa actgtaatga aacgggcgca cacgactacc acaaaacaga acacaaatca 87360
agtacctaat ggtcgagtcg aacctattca tattttgatt ggacgtcagc ttcgtaaaga 87420
aactatgcac gatgctatta aaaatttcct ggatgttaat ccagaggatt aatcaaccaa 87480
gcctgatctc agcagatcag gcttttattt ttcttgcttt ttgctaaaaa ttttggtata 87540
attcttagat aaattgatga taaggctatc ttcgtatgtg ttctagccct cttaataaat 87600
ctatatgcac tacaataaaa agatttttct tgctcaacct ggtttatctc ttcactattt 87660
gt 87662
<210> 3
<211> 57
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 3
Thr Asn Ser Glu Glu Ile Asn Gln Val Glu Gln Glu Lys Ser Phe Tyr
1 5 10 15
Cys Ser Ala Tyr Arg Phe Ile Lys Arg Ala Arg Thr His Thr Lys Ile
20 25 30
Ala Leu Ser Ser Ile Tyr Leu Arg Ile Ile Pro Lys Phe Leu Ala Lys
35 40 45
Ser Lys Lys Asn Lys Ser Leu Ile Cys
50 55
<210> 4
<211> 56
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 4
Thr Arg Ser Phe Arg Leu Leu Asn Ser Leu Leu Ile Ser Lys Met Ser
1 5 10 15
Val Asp Leu Phe Leu Trp Ser Thr Ile Ser His Asn Cys Pro Leu Ile
20 25 30
Phe Pro Arg Arg Thr Thr Ile Gln Lys His Phe Leu Gln Leu Ser Tyr
35 40 45
Leu Phe Gly Thr Asn Cys Thr Leu
50 55
<210> 5
<211> 77
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 5
Asn Ile Pro Asp Thr Cys Arg Leu Leu Thr Gln Pro Glu Asn Leu Gly
1 5 10 15
Lys Pro Phe Leu Tyr His Met Pro Asp Asn Thr Pro Lys Thr Tyr Ser
20 25 30
Tyr Thr Val Asn Arg Phe Thr Gly Tyr Ala Ala Lys Asn Gly Tyr Lys
35 40 45
Val Thr Thr Gln Lys Val Thr Leu Phe Thr Asn Ile Glu Glu Leu Pro
50 55 60
Thr Ile Ala Val Lys Leu Ile Phe Thr Lys Lys Thr Gln
65 70 75
<210> 6
<211> 180
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 6
Met Lys Cys Phe His Gly Thr Thr Gln Glu Asn Phe Leu Asn Leu Ile
1 5 10 15
Asn Asn Gly Asp Lys Pro Ser Gly Ala Trp Asn Cys Ser Asp Met Asp
20 25 30
Gly Asn Phe Tyr Val Tyr Pro Glu Asn Lys Ile Tyr Gly Asp Asp Glu
35 40 45
Glu Glu Ile Ala Ser Glu Gly Ile Gln Gln Ala Leu Gly Asn Ala Thr
50 55 60
Ile Thr Ala Ala Phe Gln Met Lys Thr Gln Asn Ile Val Ile Leu Glu
65 70 75 80
Leu Asp Ile Pro Glu Asp Glu Leu Asn Asp Asp Tyr Ser Cys Asp Asn
85 90 95
Met Ser Ser Val Ala Ser Phe Thr Glu Tyr Phe Asp Val Asn Trp Ile
100 105 110
Lys Lys Val Tyr Thr Thr Glu Phe Asn Ala Met Tyr Ser Pro Phe Cys
115 120 125
Leu Pro Ser Leu Asp Asn Pro Asn Leu Asn Tyr Leu Asp Glu Ser Leu
130 135 140
Glu Leu Leu Ala Lys Ser Val Gln Gln Ser Asp Ser Ile Gln Val Phe
145 150 155 160
Cys Asp Ile Met Asp Thr Leu Thr Gly Asn Ile Ala Glu Glu Asp Leu
165 170 175
Lys Ser Phe Phe
180
<210> 7
<211> 113
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 7
Met Ala Tyr Val Thr Val Ile Thr Asp Lys Ala Gly Ala Ser Trp Ser
1 5 10 15
Thr Gln Val Ser Asp Lys Met Ser Pro Met Gln Cys Leu Lys Tyr Phe
20 25 30
Glu Gln Trp Asn Lys Gly Glu Asp Thr Ser Pro Phe Gln Val Met Gln
35 40 45
Ile Ile His Val Asp Asn Glu Gly Asn Lys Thr Thr Leu Asn Ser Glu
50 55 60
Tyr Tyr Ala Ser Arg Phe Glu Thr Arg Ser Lys Thr Met Lys Leu Leu
65 70 75 80
Arg Glu Ser Gly Tyr Ala His Ile Ala Ala Leu Ile Trp Asp Asp Leu
85 90 95
Leu Lys Ser Gln Arg Ile Ser Tyr Val Lys Pro Glu Lys Ile Phe Ile
100 105 110
Ser
<210> 8
<211> 87
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 8
Met Glu Asn Val Glu Leu Thr Thr Leu Leu Gln Ile Ala Thr Asp Lys
1 5 10 15
Glu Asp Val Ala Leu Phe His Arg Ile Glu Ser Asp Leu Phe Asn Gly
20 25 30
Glu Ile Ile Lys Glu Ser Tyr Glu Val Val Gly Phe Lys Asp Gly Lys
35 40 45
Ala Phe Gly Phe Phe Asp Leu Asp Lys Gly Asn Ala Val Thr Lys Tyr
50 55 60
Asp Val Leu Ser Cys Glu Ile Leu Thr Lys Tyr Asn Glu Lys Arg Leu
65 70 75 80
Ala Thr Tyr Ala Asn Met Gln
85
<210> 9
<211> 100
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 9
Pro Ser Pro Leu Asn Lys Val Leu Lys Leu Leu Pro Lys Leu Glu Asn
1 5 10 15
Leu Lys Met Ala Thr Ile Arg Ala Thr Val Asn Glu Phe Gly Phe Ser
20 25 30
Ser Leu Asp Val Lys Phe Asp Ile Ala Gly Thr Asp Asn Ala Phe Glu
35 40 45
Met Val Lys Gly Leu Ile Ser Gln Thr Ile Lys Asp Tyr Asn Pro Ser
50 55 60
Asp Glu Phe Trp Glu Glu Thr Lys Gln Glu Val Leu Lys Asp Gly Tyr
65 70 75 80
Thr Tyr His Tyr Trp Gly Glu Lys Ile Val Ser Leu Tyr Tyr Asn Lys
85 90 95
Gln Asp Tyr Asn
100
<210> 10
<211> 87
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 10
Leu Glu Asn Leu Lys Met Ala Thr Ile Arg Ala Thr Val Asn Glu Phe
1 5 10 15
Gly Phe Ser Ser Leu Asp Val Lys Phe Asp Ile Ala Gly Thr Asp Asn
20 25 30
Ala Phe Glu Met Val Lys Gly Leu Ile Ser Gln Thr Ile Lys Asp Tyr
35 40 45
Asn Pro Ser Asp Glu Phe Trp Glu Glu Thr Lys Gln Glu Val Leu Lys
50 55 60
Asp Gly Tyr Thr Tyr His Tyr Trp Gly Glu Lys Ile Val Ser Leu Tyr
65 70 75 80
Tyr Asn Lys Gln Asp Tyr Asn
85
<210> 11
<211> 87
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 11
Phe His Cys Ser Asp Ala Ser Ser Asn Val Leu Glu Thr Lys Gly Arg
1 5 10 15
Gln Ile Asn Phe Lys Val Asn Cys Ala Ser Ile Val Gly Lys Phe His
20 25 30
His Phe Thr Lys Asp Thr Leu Gly Asp Phe Ile Ile Ile Arg Arg Ile
35 40 45
Leu Glu Pro Leu Phe Cys Phe Leu Phe Asn Glu Leu Ile Thr Ile Ser
50 55 60
Ile Val Ile Pro Ser Phe Phe Asn Asp Gly Gln Val Val Val Phe Leu
65 70 75 80
Ile Ile Val Leu Arg Arg Leu
85
<210> 12
<211> 106
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 12
Asp Ile Leu Phe Met Thr Cys Asp Phe Tyr Thr Asp Thr Phe Thr Arg
1 5 10 15
Ser Leu Arg Val Asp Ile Phe His Asn Arg His Thr Leu Ser Thr Lys
20 25 30
Arg Tyr His Ser Asp His Phe Thr Val Leu Leu Phe Lys Val His Phe
35 40 45
Asn Phe Leu Gln Ser Val Phe Ile Glu Arg Ile Arg Pro Phe Tyr Lys
50 55 60
Asp Ser Phe Lys Trp Leu Phe Thr Ile Phe Asn Phe Ser Val Met Pro
65 70 75 80
Tyr Phe Lys Asp Asn Leu Phe Glu Pro Val Lys Ile Phe Phe Gly Glu
85 90 95
Cys Phe Ser Asn Ile Thr Pro Val Lys Tyr
100 105
<210> 13
<211> 102
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 13
Met Thr Cys Asp Phe Tyr Thr Asp Thr Phe Thr Arg Ser Leu Arg Val
1 5 10 15
Asp Ile Phe His Asn Arg His Thr Leu Ser Thr Lys Arg Tyr His Ser
20 25 30
Asp His Phe Thr Val Leu Leu Phe Lys Val His Phe Asn Phe Leu Gln
35 40 45
Ser Val Phe Ile Glu Arg Ile Arg Pro Phe Tyr Lys Asp Ser Phe Lys
50 55 60
Trp Leu Phe Thr Ile Phe Asn Phe Ser Val Met Pro Tyr Phe Lys Asp
65 70 75 80
Asn Leu Phe Glu Pro Val Lys Ile Phe Phe Gly Glu Cys Phe Ser Asn
85 90 95
Ile Thr Pro Val Lys Tyr
100
<210> 14
<211> 154
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 14
Arg Leu Val Gln Tyr Glu Pro Val Ala Phe Lys Asn Ser Asn Asn Lys
1 5 10 15
Ile Leu Asp Asp Glu Ser Val Leu Phe Thr Ile Gly Cys Leu His Arg
20 25 30
Tyr Arg Ser Asp Tyr Glu Phe Ile Lys Phe Leu Tyr Lys Gly Asp His
35 40 45
Tyr Val Gln Gln Val Glu Gly Glu Ala Val Ile Glu Gly Thr Ile Phe
50 55 60
Asn Lys Leu Lys Tyr Val Asn Gly Val Lys Pro Val Thr Lys Met Glu
65 70 75 80
Pro Glu Lys Leu Ala Val Gly Ile Lys Ala Asp Gly Leu Leu His Ser
85 90 95
Ser Glu Phe Ala Val His Lys Met Tyr Ala Glu Ser Cys Glu Trp Ile
100 105 110
Phe Cys His Val Leu Val Lys Ala Ala His Arg Ser Cys Asn Leu Lys
115 120 125
Asp Cys Ile Glu Thr Tyr Met Glu Asp Lys Thr Ser Asn Asp Leu Met
130 135 140
Ala Arg Gln Val Ile Glu Lys Val Lys Met
145 150
<210> 15
<211> 151
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 15
Ala Asn Ser Lys Ala Ser Asp Arg Lys Ser Glu Tyr Ala Arg Arg Ile
1 5 10 15
Val Arg Asn Leu Met Glu Leu Thr Leu Lys Gly Ser Glu Leu His Pro
20 25 30
Met Val Leu Lys Leu Ala Lys Glu Val Asp His Val Val Asp Ile Ile
35 40 45
Gly Glu Glu Tyr Ala Glu Val Leu Ile Asp Lys Val Asn Gln Gln Asn
50 55 60
Asp Lys Gln Pro Ile Ala Leu Arg Met Asn Phe Glu Asn Ala Gln Pro
65 70 75 80
His Asp Ser Gly Arg Trp Tyr Gly Asp Lys Asn Phe Ser Ile Tyr Asp
85 90 95
Gly His Tyr Phe His Val Gly Asn Ala Lys Phe Lys Glu Leu Val Leu
100 105 110
Cys Gln Ile Pro Tyr Glu Thr Asn Trp Glu Arg Val Gly Ile Val Lys
115 120 125
Val Thr Ala Ile Tyr Glu Gln Thr Lys Ala Asp Ile Ile Asp Leu Glu
130 135 140
Gln Gly Ala Ile Ile Gln Met
145 150
<210> 16
<211> 74
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 16
Glu Ser Lys Glu Thr Lys Lys Asp Ser Lys Lys Ser Thr Lys Lys Glu
1 5 10 15
Ala Lys Gln Glu Pro Glu Lys Glu Ser Thr Ala Lys Ile Val Met Asp
20 25 30
Leu Leu Glu Ser Leu Asp Ser Lys Leu Leu Thr Val Pro Gln Glu Asn
35 40 45
Tyr Phe Asn Lys Asp Leu Tyr Val Lys Phe Glu Glu Ile Ser Pro Phe
50 55 60
Gly Phe Ile Pro Val Pro Thr Lys Val Met
65 70
<210> 17
<211> 193
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 17
Ser Tyr Lys Lys Pro Leu Leu Ala Ser Arg Lys Asn Ser Glu Glu Ser
1 5 10 15
Leu Leu Ala Ile Phe Glu Asp Gly Lys Gly Lys Trp Pro Ser Phe Thr
20 25 30
Pro Ile His Glu Arg Ile Ala Asp Ile Val Thr Lys Leu Ala Asn Asn
35 40 45
Lys Leu Trp Trp Asp Ser Val Ala Ser Ala Val Ile Tyr Tyr Ala Gly
50 55 60
Asp Asp Thr Phe Ile Pro Glu Ser Ile Cys Pro Ser Ile Met Thr Thr
65 70 75 80
Ile Ile Ser Gly Asn Asn Met Tyr Tyr Thr Arg Lys Glu Val Lys Asp
85 90 95
Leu Leu Lys His Leu Asp Ala Phe Leu Ser Val Val Glu Lys His Asp
100 105 110
Ile Asp Lys Glu Leu Asn Ala Arg Tyr Lys Leu Leu Ala Ala Asp Val
115 120 125
Glu Lys Asp Asn Phe Lys Phe Tyr Gln Val Glu Glu Asn Val Cys Val
130 135 140
Ser Ile Asn Asn Thr Asn Ile Thr Leu Lys Cys Leu Lys Val Pro Ser
145 150 155 160
Asn Ile Asn Thr Gln Phe Leu Ala Val Cys Gly Glu Val Asn Gly Phe
165 170 175
His Lys Val Leu Thr Val Glu Pro Gln Ile His Glu His Glu Glu Ser
180 185 190
Met
<210> 18
<211> 69
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 18
Leu Ser Ile Ser Tyr Ile Lys Lys Ser Leu Leu Arg Cys Lys Ser Ser
1 5 10 15
Leu Phe Val Lys Asp Ser Leu Ile Ser Glu Lys Phe Leu Tyr Thr Met
20 25 30
Thr Trp Ser Leu Leu Ile Val Lys Leu Ser Asp Glu Glu Ile Val Asp
35 40 45
Phe Val Ile Thr Tyr His Lys Val Lys Ala Gly Ile Phe Lys Arg Phe
50 55 60
Val Val Gly Ile Val
65
<210> 19
<211> 97
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 19
Glu Asp Asn Phe Glu Arg Val Phe Phe Tyr Asn Val Lys Asn Asn Gly
1 5 10 15
Val Met Leu Asp Phe Cys Thr Asn Glu Phe Pro Lys Asn Tyr Ala Asp
20 25 30
Tyr Ser Ile Tyr Pro Asp Ala Val Gln Lys Thr Ser Leu Lys Lys Ser
35 40 45
Ser Thr Asn Leu Leu Gly Gly Tyr Leu Lys Lys Ile Ala Glu Glu Val
50 55 60
Val Ser Trp Ile Ile Glu Asp Thr Ile Glu Glu Tyr Leu Asp Leu Glu
65 70 75 80
Arg Glu Leu Ser Phe Asp Cys Ile Pro Phe Val Val Gln Val Lys Ser
85 90 95
Met
<210> 20
<211> 113
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 20
Leu His Tyr Lys Ala Glu Gln Glu His Thr Ala Leu Gln Arg Glu Leu
1 5 10 15
Val Ser Lys Tyr Tyr Gly Ala Leu Gln Glu Lys Thr Leu Pro His Glu
20 25 30
Ser Ile Ile Asp Leu Gly Ser Glu Gly Leu Pro Asn Phe Gln Gly His
35 40 45
Glu Asn Val Asn Tyr Thr Glu Phe Lys Phe Leu Gly Asn Asn Val Ser
50 55 60
Glu Ile Pro Ser Leu Glu Val Leu Glu Val Thr Phe Arg Thr Tyr Val
65 70 75 80
Lys Asp Gly Arg Ser Val Val Asn Asn Gly Val Ile Arg Val Leu Ser
85 90 95
Gly Asn Arg Thr Tyr Lys Phe Thr Gly Phe Asp Ser Ala Trp Leu Lys
100 105 110
Met
<210> 21
<211> 120
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 21
Leu Leu Asn Leu Phe Leu Arg Val Ala Leu Cys Leu Pro Cys Trp Val
1 5 10 15
Asn Cys Asn Cys Cys Cys Ile Gly Cys Tyr Arg Ala Thr Cys Thr Ser
20 25 30
Asn Tyr Thr Gly Val Thr Ser Cys Ile Ser Ser Gly Cys Thr Phe Phe
35 40 45
Gly Val Ser Cys Ser Ser Cys Ile Glu Asp Val Phe Leu Thr Phe Leu
50 55 60
Pro Leu Val Thr Cys Arg Thr Cys Cys His Asn Ile Gln Arg Gln Gly
65 70 75 80
Leu Thr Phe Ser Asp Phe Leu Ile Gly Trp Leu Leu Ser Asp Tyr Trp
85 90 95
Lys Cys Ile Tyr Ser Asn Cys Gly Cys Val Arg Cys Asp Arg Ala Thr
100 105 110
Cys Thr Cys Tyr Asn Ala Ser Val
115 120
<210> 22
<211> 154
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 22
Lys Pro Tyr Val Ser Asp Leu Tyr Ile Asp Ala Glu Met Arg Arg Arg
1 5 10 15
Asn Lys Leu Gly Asn Ser Thr Val Lys Lys Gly Lys Val Thr Val Lys
20 25 30
Asn Trp Trp Gln Met Ala Glu Ala Cys Gly Val Lys Asn Gly Asp Asn
35 40 45
His Arg Lys Ile Phe Thr Ser Asp Gln Met Ala Thr Ile Gly Ile Asn
50 55 60
Tyr Thr Glu Ser Val Leu Ala Asp Phe Glu Asn Gln Ser Thr Pro Val
65 70 75 80
Lys Ile Ala Lys Asn Val Ala Ala Val Phe Lys Glu Val Asp Ala Leu
85 90 95
Leu Tyr Gln Glu Ala Gln Glu Ala Thr Ile Lys Met Gly Met Lys Val
100 105 110
Pro Lys Gly Ala Ile Arg Ile Thr Gly Tyr Gly Ile Thr Pro Ile Gly
115 120 125
Ala Ser Asp Glu Tyr Ala Glu Leu Lys Leu Gly Glu Phe Phe Lys Ile
130 135 140
Ala Glu Leu Gly Lys Arg Ser Leu Gln Met
145 150
<210> 23
<211> 160
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 23
Lys Pro Tyr Val Ser Asp Leu Tyr Ile Asp Ala Glu Met Arg Arg Arg
1 5 10 15
Asn Lys Leu Gly Asn Ser Thr Val Lys Lys Gly Lys Val Thr Val Lys
20 25 30
Asn Trp Trp Gln Met Ala Glu Ala Cys Gly Val Lys Asn Gly Asp Asn
35 40 45
His Arg Lys Ile Phe Thr Ser Asp Gln Met Ala Thr Ile Gly Ile Asn
50 55 60
Tyr Thr Glu Ser Val Leu Ala Asp Phe Glu Asn Gln Ser Thr Pro Val
65 70 75 80
Lys Ile Ala Lys Asn Val Ala Ala Val Phe Lys Glu Val Asp Ala Leu
85 90 95
Leu Tyr Gln Glu Ala Gln Glu Ala Thr Ile Lys Met Gly Met Lys Val
100 105 110
Pro Lys Gly Ala Ile Arg Ile Thr Gly Tyr Gly Ile Thr Pro Ile Gly
115 120 125
Ala Ser Asp Glu Tyr Ala Glu Leu Lys Leu Gly Glu Phe Phe Lys Ile
130 135 140
Ala Glu Leu Gly Lys Arg Ser Leu Gln Met Ile Gly Gly Arg Ser Pro
145 150 155 160
<210> 24
<211> 55
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 24
Arg His Val Leu Val Thr Phe Ser Asn Met Val Leu Leu Phe Lys Ile
1 5 10 15
Thr Gln Asn Ile Thr Leu Ser Arg Leu Lys Gln Leu Ile Leu Lys Leu
20 25 30
Gly Ile Arg Ala Ile Pro Leu His Tyr Phe Leu Arg Lys Ile His Glu
35 40 45
Leu Ile Met Gly Arg Val Leu
50 55
<210> 25
<211> 377
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 25
Ala Glu Gly Ala Pro Asn Val Thr Val Thr Ala Ala Asn Ser Thr Val
1 5 10 15
Ser Gly Gly Ala Gly Thr Val Val Cys Thr Tyr Ser Gly Ala Asp Ala
20 25 30
Thr Thr Ala Gly Glu Lys Thr Tyr Thr Ala Thr Thr Ala Asp Leu Ile
35 40 45
Asp Glu Glu Gly Lys Lys Trp Gln Tyr Gly Thr Ala Gly Thr Ala Val
50 55 60
Val Asn Leu Thr Leu Thr Glu Gly Glu Thr Ile Glu Gln Asn Thr Pro
65 70 75 80
Gln Gln Thr Ile Val Pro Leu Ala Asn Val Thr Val Thr Ala Ala Asn
85 90 95
Ser Thr Val Ser Gly Gly Ala Gly Thr Val Val Cys Thr Tyr Ser Gly
100 105 110
Ala Asp Thr Pro Leu Ala Gly Ser Lys Ser Tyr Thr Ala Asn Thr Ala
115 120 125
Ser Pro Ile Asp Ser Gly Asp Lys Lys Trp Gln Tyr Gly Thr Ala Gly
130 135 140
Thr Ala Thr Val Glu Leu Ser Ile Asn Gly Gly Glu Asn Ile Thr Gln
145 150 155 160
Ser Ser Pro Gln Gln Thr Ile Val Pro Leu Ala Asn Val Thr Val Thr
165 170 175
Ala Ala Asp Ser Thr Val Ser Thr Gly Ala Glu Gly Ser Val Val Cys
180 185 190
Thr Tyr Leu Gly Ala Asp Ser Pro Ala Val Ser Gln Lys Thr Tyr Thr
195 200 205
Ala Ser Asn Ala Ser Pro Ile Asp Glu Gly Asp Lys Lys Trp Gln Tyr
210 215 220
Gly Thr Ala Asn Thr Ala Leu Ile Ser Leu Thr Leu Thr Asp Gly Glu
225 230 235 240
Asn Val Thr Gln Gly Thr Pro Gln Gln Thr Ile Ala Pro Pro Lys Ile
245 250 255
Ala Asp Lys Leu Lys Ala Asn Leu Met Ser Phe Pro Asn Val Arg Ala
260 265 270
Leu Trp Glu Cys Tyr Ile Thr Asp Val Asp Lys Gly Asn Ile Ile Ala
275 280 285
Tyr Gln Leu Asp Met Phe Phe Asp Ala Thr Arg Met Gln Ala Gly Val
290 295 300
Ser Tyr Leu Leu Glu Asp Ser Phe Pro Val Lys Tyr Asp Pro Lys Tyr
305 310 315 320
Asp Phe Lys Glu Val Glu Ala Ile Asp Ala Glu Pro Trp Tyr Ser Ser
325 330 335
Tyr Thr Leu Pro Leu Ile Ala Glu Gln Tyr Thr Arg Phe Asp Tyr Arg
340 345 350
Lys Gly Ala Lys Val Asn Ala Val Phe Gly Lys Val Phe Glu Lys Phe
355 360 365
Asp Pro Tyr Thr Lys Ala Lys Ile Met
370 375
<210> 26
<211> 379
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 26
Ala Glu Gly Ala Pro Asn Val Thr Val Thr Ala Ala Asn Ser Thr Val
1 5 10 15
Ser Gly Gly Ala Gly Thr Val Val Cys Thr Tyr Ser Gly Ala Asp Ala
20 25 30
Thr Thr Ala Gly Glu Lys Thr Tyr Thr Ala Thr Thr Ala Asp Leu Ile
35 40 45
Asp Glu Glu Gly Lys Lys Trp Gln Tyr Gly Thr Ala Gly Thr Ala Val
50 55 60
Val Asn Leu Thr Leu Thr Glu Gly Glu Thr Ile Glu Gln Asn Thr Pro
65 70 75 80
Gln Gln Thr Ile Val Pro Leu Ala Asn Val Thr Val Thr Ala Ala Asn
85 90 95
Ser Thr Val Ser Gly Gly Ala Gly Thr Val Val Cys Thr Tyr Ser Gly
100 105 110
Ala Asp Thr Pro Leu Ala Gly Ser Lys Ser Tyr Thr Ala Asn Thr Ala
115 120 125
Ser Pro Ile Asp Ser Gly Asp Lys Lys Trp Gln Tyr Gly Thr Ala Gly
130 135 140
Thr Ala Thr Val Glu Leu Ser Ile Asn Gly Gly Glu Asn Ile Thr Gln
145 150 155 160
Ser Ser Pro Gln Gln Thr Ile Val Pro Leu Ala Asn Val Thr Val Thr
165 170 175
Ala Ala Asp Ser Thr Val Ser Thr Gly Ala Glu Gly Ser Val Val Cys
180 185 190
Thr Tyr Leu Gly Ala Asp Ser Pro Ala Val Ser Gln Lys Thr Tyr Thr
195 200 205
Ala Ser Asn Ala Ser Pro Ile Asp Glu Gly Asp Lys Lys Trp Gln Tyr
210 215 220
Gly Thr Ala Asn Thr Ala Leu Ile Ser Leu Thr Leu Thr Asp Gly Glu
225 230 235 240
Asn Val Thr Gln Gly Thr Pro Gln Gln Thr Ile Ala Pro Pro Lys Ile
245 250 255
Ala Asp Lys Leu Lys Ala Asn Leu Met Ser Phe Pro Asn Val Arg Ala
260 265 270
Leu Trp Glu Cys Tyr Ile Thr Asp Val Asp Lys Gly Asn Ile Ile Ala
275 280 285
Tyr Gln Leu Asp Met Phe Phe Asp Ala Thr Arg Met Gln Ala Gly Val
290 295 300
Ser Tyr Leu Leu Glu Asp Ser Phe Pro Val Lys Tyr Asp Pro Lys Tyr
305 310 315 320
Asp Phe Lys Glu Val Glu Ala Ile Asp Ala Glu Pro Trp Tyr Ser Ser
325 330 335
Tyr Thr Leu Pro Leu Ile Ala Glu Gln Tyr Thr Arg Phe Asp Tyr Arg
340 345 350
Lys Gly Ala Lys Val Asn Ala Val Phe Gly Lys Val Phe Glu Lys Phe
355 360 365
Asp Pro Tyr Thr Lys Ala Lys Ile Met Glu Lys
370 375
<210> 27
<211> 123
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 27
Lys Lys Glu Ala Glu Leu His Ala Asn Leu Arg Phe Tyr Gly Lys His
1 5 10 15
Asn Pro Ser Val Asp Gly Val Val Ile Gly Lys Val Lys Tyr Gly Lys
20 25 30
Gly Ile Val Tyr Gln Ile Asp Met Leu Phe Arg Gly Leu Gly Val Ile
35 40 45
His Phe Met Pro Tyr Leu Leu Asn Glu Ser Asp Pro Ile Asn Ile Glu
50 55 60
Pro Lys Tyr Thr Arg Gln Glu Ser Thr Pro Thr Ile Asp Glu Glu Pro
65 70 75 80
Trp Lys Ser Leu Arg Trp Thr Leu Pro Thr Pro Ile Val Asp Asn Leu
85 90 95
Asn Tyr Arg Glu Gly Asn Gln Val Ser Asn Ile Tyr Ser Gln Leu Phe
100 105 110
Cys Tyr Phe Asp Lys Ala Ser Lys Ala Lys Met
115 120
<210> 28
<211> 60
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 28
Asn Pro Val Val Cys Leu Tyr Asp Glu Gln Arg Arg Tyr Asn Gln Phe
1 5 10 15
Phe Leu Tyr Leu Gly Ile Glu Ser Ser Ser Tyr Tyr Ile Leu Glu Val
20 25 30
Arg Lys Ser Glu Lys Cys His Asn Thr Asn Lys Gly Lys Lys Pro Lys
35 40 45
Asn Ser Lys Arg Met Leu Asp Gly Lys Tyr Phe Leu
50 55 60
<210> 29
<211> 123
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 29
Met Lys Asn Arg Thr Asn Lys Gly Gln Phe Lys Lys Gly Gln Ser Gly
1 5 10 15
Asn Pro Ser Gly Arg Pro Lys Gly Ser Arg Asn Lys Ser Ser Leu Val
20 25 30
Lys Ala Gln Leu Thr Ile Asp Asn Ser Ala Glu Phe Ala Ala Lys Leu
35 40 45
Phe Glu Ala Ile Val Thr Arg Asp Ala Ala Lys Leu Ala Glu Phe Gly
50 55 60
Leu Asn Thr Asp Asp Val Thr Ile Lys Ser Met Ile Glu Val Gly Lys
65 70 75 80
Thr Ile Met Thr His Ser Ala Gly Glu Met Lys Ala Ile Ala Ala Asp
85 90 95
Thr Lys Lys Thr Pro Asp Asn Gly Gly Gln Ser Gln Thr Asp Asn Lys
100 105 110
Pro Thr Phe Ser Ala Val Ala Thr Leu Lys Lys
115 120
<210> 30
<211> 56
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 30
Val His Ala Gly Glu His Leu Asp Phe Gln Phe His His Gln Leu Gly
1 5 10 15
Tyr Lys Met Leu Thr Gln Glu Gln Leu Ser Lys Cys Leu Gln Gln Asn
20 25 30
Leu Gly Tyr Cys His Leu Phe Arg Val Leu Gly Thr Arg Cys Leu Lys
35 40 45
Ala His Pro His Gln His Gln Leu
50 55
<210> 31
<211> 61
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 31
Leu Cys Ser Lys Val Thr Asn Lys Leu Cys Thr Phe Ser Val Val Gln
1 5 10 15
Ser Leu Phe Ile His Trp Thr Leu Lys Asp Tyr Glu Arg Ile His Leu
20 25 30
Glu Ile Leu Asn Ile Ser Ser Val Thr Thr Cys Val Lys Phe Cys Asn
35 40 45
Leu Ala Leu Val Phe Phe Ser Ser Ile Leu His Trp Leu
50 55 60
<210> 32
<211> 62
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 32
Leu Leu Tyr Leu Phe Ala Pro Tyr Ser Ile Gly Cys Ser Thr Asn Gln
1 5 10 15
Tyr Lys Ala Phe Arg Leu Pro Val Gln Ser Thr Asp Thr Leu Ser Leu
20 25 30
Pro Thr Ala Ile Thr Asp Pro Lys Glu Gly Ala Val Lys Phe Ala Arg
35 40 45
Ser Pro Pro Ser Phe Asn Ser Gly Ile Leu Thr Ile Ser Leu
50 55 60
<210> 33
<211> 57
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 33
Leu Arg Gly Leu Phe Ser Pro Ser Leu Lys Gly Phe Leu Arg Val Ser
1 5 10 15
Phe Lys Gly Ala Glu Asp Gln Ile Gln Gln Val Arg Met Ile Phe Ser
20 25 30
Ser Gln Phe Asn Phe Glu Lys Phe Lys Thr Trp Ser Gly Ser Thr Asn
35 40 45
Leu Gln Lys Thr Val Tyr Ser Lys Thr
50 55
<210> 34
<211> 59
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 34
Met Val Met Arg Gln Thr Phe Asn Leu Thr Gly Asp Gly Phe Glu Ser
1 5 10 15
Leu His Phe Tyr Gln Tyr Gly Ser Val Ala Asp Lys Val Met Gln Gly
20 25 30
Phe His Lys Pro Tyr Glu Tyr Gly Phe Asp Ser His Leu Asn Leu Gln
35 40 45
Leu Trp Val Ser Ile Asn Gly Asn Ala Asn Gly
50 55
<210> 35
<211> 58
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 35
His Met Trp Leu Lys Phe Pro Phe Ile Val Thr Lys Trp Val Ser Thr
1 5 10 15
Ser Pro Glu Ile Gly Glu Pro His Glu Leu Asn Ile Ser Ser Glu Tyr
20 25 30
Leu Gln Ser Asn Cys Met Gly Gln Leu Ser Pro Ser Ala Pro Glu Leu
35 40 45
Gly Thr Leu Leu Val Leu Glu Ser Thr Leu
50 55
<210> 36
<211> 59
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 36
Phe Leu Leu Leu Leu Ala Arg Ser Tyr Thr Asn Lys Arg Glu Ser Gly
1 5 10 15
Phe Leu Ile Lys Leu Cys Pro Lys Lys Val Ser Asn Tyr Lys Ile Leu
20 25 30
Leu Ser Ile Cys Tyr Thr Glu Ser Thr Cys Gln Pro Lys Glu Lys Val
35 40 45
Thr Glu Thr Gly Asp Lys Lys Leu Pro Phe Met
50 55
<210> 37
<211> 540
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 37
Phe Tyr Leu Arg Arg Arg Leu Met Asp Leu Asn Ala Val Lys Gln Lys
1 5 10 15
Arg Val Glu Asp Val Arg Lys Val Leu Ala Gly Glu Leu Gly Leu Ser
20 25 30
Asp Glu Val Lys Glu Ile Ile Lys Ser Phe Gly Lys Asp Pro Ser Lys
35 40 45
Phe Leu Pro Thr Gln Ile Leu Thr Leu Leu Arg Tyr Thr Pro Asp Gln
50 55 60
Val Arg Leu Ile Phe Lys Leu Met Thr Asp Lys Asn Tyr Val Ala Pro
65 70 75 80
Gln Pro Gly Ser Gln Glu Val Phe Leu Asn Thr Asn Ala Asp Leu Val
85 90 95
Leu Tyr Gly Gly Ala Ala Gly Ala Gly Lys Thr Ala Ala Leu Leu Met
100 105 110
Asp Ser Leu Arg Phe Ile Glu Asp Pro Asn Tyr Asn Ala Val Tyr Phe
115 120 125
Arg Arg Asn Thr Thr Gln Leu Gln Gly Gly Leu Trp Pro Ala Ala Lys
130 135 140
Lys Leu Phe Gly Lys Phe Gly Gly Ile Pro His Glu Gln Lys Met Thr
145 150 155 160
Ile Thr Phe Pro Ser Gly Ala Thr Ile Lys Phe Thr Tyr Leu Glu Leu
165 170 175
Glu Lys His Ala Glu Gly His Gln Gly Ile Glu Tyr Ser Ala Ile Tyr
180 185 190
Phe Asp Glu Gly Thr His Phe Ser Ala Ser Gln Ile Ser Tyr Leu Gln
195 200 205
Thr Arg Leu Arg Ser Gly Ala Glu Gly Asp Ser Tyr Met Lys Ile Ser
210 215 220
Met Asn Pro Asp Arg Asp His Phe Ile Tyr Asp Trp Val Glu Pro Phe
225 230 235 240
Leu Asp Glu Glu Gly Tyr Pro Asp Pro Glu Lys Cys Gly Arg Ile Arg
245 250 255
Trp Tyr Val Met Asn Asp Gly Val Met Val Ser Asp Trp Glu Arg Asp
260 265 270
Lys Ile Leu Glu Met Phe Pro Leu Glu Ile Pro Gln Thr Tyr Thr Phe
275 280 285
Ile Ser Gly Thr Ile Asp Asp Asn Pro Ile Leu Asp Phe Leu Glu Pro
290 295 300
Lys Tyr Arg Gly Lys Leu Glu Asn Asn Thr Pro Val Asn Val Ala Arg
305 310 315 320
Leu Arg Phe Gly Asn Trp Lys Ala Arg Ala Glu Gly Ser Asn Tyr Trp
325 330 335
Gln Arg Gln Trp Cys Glu Ile Val Asp Ser Leu Pro Glu Asp Val Phe
340 345 350
Asp Val Arg Ala Trp Asp Leu Ala Ala Thr Leu Pro Ser Glu Ile Asn
355 360 365
Pro Asn Pro Asp Trp Thr Ala Gly Val Lys Met Gly Lys Ser Lys Lys
370 375 380
Asp Gly Cys Tyr Tyr Ile Ile Asp Val Val Arg Phe Arg Asp Arg Pro
385 390 395 400
Ser Gly Val Glu Thr Gln Ile Asn Leu Thr Ala Glu Ser Asp Gly Lys
405 410 415
Arg Thr Gly Ile Phe Ile Pro Gln Asp Pro Gly Ala Ala Gly Lys Ser
420 425 430
Tyr Ala Thr Ser Leu Ile Arg Lys Leu Ala Glu Lys Gly Tyr Arg Ala
435 440 445
Arg Ala Lys Pro Thr Asn Lys Asp Lys Val Thr Arg Phe Ala Gly Phe
450 455 460
Ser Ala Ala Ser Glu Ala Gly Leu Val Lys Val Leu Arg Gly Ser Trp
465 470 475 480
Asn Glu Ala Tyr Phe Gln Glu Leu Glu Gly Phe Cys Gly Asp Gly Lys
485 490 495
Thr Lys Asp Asp Gln Val Asp Ala Thr Ser Asp Ala Phe Asn Ser Leu
500 505 510
Asn Glu Val Lys Leu Phe Lys Pro Pro Ser Met Gly Ala His Thr Asp
515 520 525
Leu Val Arg Gly Asn Pro Tyr Glu Gly Leu Arg Arg
530 535 540
<210> 38
<211> 538
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 38
Leu Arg Arg Arg Leu Met Asp Leu Asn Ala Val Lys Gln Lys Arg Val
1 5 10 15
Glu Asp Val Arg Lys Val Leu Ala Gly Glu Leu Gly Leu Ser Asp Glu
20 25 30
Val Lys Glu Ile Ile Lys Ser Phe Gly Lys Asp Pro Ser Lys Phe Leu
35 40 45
Pro Thr Gln Ile Leu Thr Leu Leu Arg Tyr Thr Pro Asp Gln Val Arg
50 55 60
Leu Ile Phe Lys Leu Met Thr Asp Lys Asn Tyr Val Ala Pro Gln Pro
65 70 75 80
Gly Ser Gln Glu Val Phe Leu Asn Thr Asn Ala Asp Leu Val Leu Tyr
85 90 95
Gly Gly Ala Ala Gly Ala Gly Lys Thr Ala Ala Leu Leu Met Asp Ser
100 105 110
Leu Arg Phe Ile Glu Asp Pro Asn Tyr Asn Ala Val Tyr Phe Arg Arg
115 120 125
Asn Thr Thr Gln Leu Gln Gly Gly Leu Trp Pro Ala Ala Lys Lys Leu
130 135 140
Phe Gly Lys Phe Gly Gly Ile Pro His Glu Gln Lys Met Thr Ile Thr
145 150 155 160
Phe Pro Ser Gly Ala Thr Ile Lys Phe Thr Tyr Leu Glu Leu Glu Lys
165 170 175
His Ala Glu Gly His Gln Gly Ile Glu Tyr Ser Ala Ile Tyr Phe Asp
180 185 190
Glu Gly Thr His Phe Ser Ala Ser Gln Ile Ser Tyr Leu Gln Thr Arg
195 200 205
Leu Arg Ser Gly Ala Glu Gly Asp Ser Tyr Met Lys Ile Ser Met Asn
210 215 220
Pro Asp Arg Asp His Phe Ile Tyr Asp Trp Val Glu Pro Phe Leu Asp
225 230 235 240
Glu Glu Gly Tyr Pro Asp Pro Glu Lys Cys Gly Arg Ile Arg Trp Tyr
245 250 255
Val Met Asn Asp Gly Val Met Val Ser Asp Trp Glu Arg Asp Lys Ile
260 265 270
Leu Glu Met Phe Pro Leu Glu Ile Pro Gln Thr Tyr Thr Phe Ile Ser
275 280 285
Gly Thr Ile Asp Asp Asn Pro Ile Leu Asp Phe Leu Glu Pro Lys Tyr
290 295 300
Arg Gly Lys Leu Glu Asn Asn Thr Pro Val Asn Val Ala Arg Leu Arg
305 310 315 320
Phe Gly Asn Trp Lys Ala Arg Ala Glu Gly Ser Asn Tyr Trp Gln Arg
325 330 335
Gln Trp Cys Glu Ile Val Asp Ser Leu Pro Glu Asp Val Phe Asp Val
340 345 350
Arg Ala Trp Asp Leu Ala Ala Thr Leu Pro Ser Glu Ile Asn Pro Asn
355 360 365
Pro Asp Trp Thr Ala Gly Val Lys Met Gly Lys Ser Lys Lys Asp Gly
370 375 380
Cys Tyr Tyr Ile Ile Asp Val Val Arg Phe Arg Asp Arg Pro Ser Gly
385 390 395 400
Val Glu Thr Gln Ile Asn Leu Thr Ala Glu Ser Asp Gly Lys Arg Thr
405 410 415
Gly Ile Phe Ile Pro Gln Asp Pro Gly Ala Ala Gly Lys Ser Tyr Ala
420 425 430
Thr Ser Leu Ile Arg Lys Leu Ala Glu Lys Gly Tyr Arg Ala Arg Ala
435 440 445
Lys Pro Thr Asn Lys Asp Lys Val Thr Arg Phe Ala Gly Phe Ser Ala
450 455 460
Ala Ser Glu Ala Gly Leu Val Lys Val Leu Arg Gly Ser Trp Asn Glu
465 470 475 480
Ala Tyr Phe Gln Glu Leu Glu Gly Phe Cys Gly Asp Gly Lys Thr Lys
485 490 495
Asp Asp Gln Val Asp Ala Thr Ser Asp Ala Phe Asn Ser Leu Asn Glu
500 505 510
Val Lys Leu Phe Lys Pro Pro Ser Met Gly Ala His Thr Asp Leu Val
515 520 525
Arg Gly Asn Pro Tyr Glu Gly Leu Arg Arg
530 535
<210> 39
<211> 56
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 39
Arg Gly Leu Val Gln Phe Ala Arg Gln Leu Asp Asp Pro Tyr Gln Ile
1 5 10 15
Ser Leu Leu Arg Ser Gln Arg Leu Tyr Val Ser Arg Gln Lys Val Ser
20 25 30
Lys Met Gly Val Ser Glu Asp Ala Asn Gln His Gln Leu His Asn Met
35 40 45
Cys Ser Ser Lys Trp Ser Gly Leu
50 55
<210> 40
<211> 488
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 40
Met Ala Asp Ile Thr Glu Thr Gln Glu Ser Leu Pro Pro Phe Arg Met
1 5 10 15
Gly Glu Val Gly Ser Leu Gly Leu Lys Val Lys Asn Gly Arg Ile Tyr
20 25 30
Glu Glu Pro Arg Gln Ala Leu Arg Phe Pro Glu Ser Ile Lys Thr Phe
35 40 45
Gln Leu Met Met Arg Asp Pro Ala Val Ala Ala Ser Val Asn Ile Ile
50 55 60
Lys Met Phe Val Arg Lys Val Asn Trp Arg Phe Val Pro Pro Lys Gly
65 70 75 80
Lys Glu Gln Asp Pro Lys Met Leu Glu Arg Ala Asp Phe Phe Asn Ser
85 90 95
Leu Met Asp Asp Met Glu His Asp Trp Ala Asp Phe Ile Asn Ser Val
100 105 110
Met Ser Phe Cys Thr Tyr Gly Phe Cys Val Asn Glu Lys Val Tyr Lys
115 120 125
Lys Arg Gln Gly Lys Lys Gly Lys Tyr Gln Ser Lys Phe Asp Asp Gly
130 135 140
Leu Ile Gly Trp Ala Lys Leu Pro Ile Arg Asn Gln Ser Thr Leu Asp
145 150 155 160
Lys Trp Tyr Phe Asp Glu Asp Phe Arg Lys Val Thr Gly Val Arg Gln
165 170 175
Asn Leu Arg Asn Val Ser His Ile Ala Gly Ala Ile Asn Leu Gly Glu
180 185 190
Arg Pro Leu Thr Arg Lys Leu Pro Arg Ala Lys Phe Met Leu Phe Lys
195 200 205
Tyr Asp Asp Glu Tyr Gly Asn Pro Glu Gly Arg Ser Pro Leu Leu Asn
210 215 220
Ala Tyr Val Pro Trp Lys Tyr Lys Val Gln Ile Glu Glu Tyr Glu Ala
225 230 235 240
Val Gly Val Ser Arg Asp Leu Val Gly Met Pro Lys Ile Gly Leu Pro
245 250 255
Pro Asp Tyr Leu Asp Glu Asn Ala Glu Pro Glu Lys Lys Ala Phe Val
260 265 270
Gln Tyr Cys Lys Thr Val Val Asn Asp Met Ile Ala Asn Asp Arg Ala
275 280 285
Gly Leu Ile Trp Pro Arg Tyr Ile Asp Pro Asp Thr Lys Glu Asp Ile
290 295 300
Phe Glu Phe Ser Leu Val Ser Arg Gln Gly Ala Lys Ala Tyr Asp Thr
305 310 315 320
Gly Ser Ile Ile Asp Arg Tyr Ser Lys Gln Ile Met Met Ala Phe Met
325 330 335
Ser Asp Val Leu Ala Met Gly Gln Ser Lys Tyr Gly Ser Phe Ser Leu
340 345 350
Ala Asp Ser Lys Thr Ser Leu Leu Ala Met Ser Val Asp Ile Leu Leu
355 360 365
Lys Gln Ile Lys Asn Val Ile Asn Arg Asp Leu Val Ala Gln Thr Tyr
370 375 380
Ala Leu Asn Met Trp Asp Asp Glu Glu His Val Gln Ile Thr Tyr Asp
385 390 395 400
Asp Ile Glu Thr Pro Asp Leu Glu Ala Ile Gly Ser Tyr Ile Gln Lys
405 410 415
Thr Val Ala Val Gly Ala Leu Glu Val Asp Lys Glu Leu Ser Asn Lys
420 425 430
Leu Arg Glu His Ile Gly Leu Pro Pro Ala Asp Glu Ser Gln Pro Val
435 440 445
Ser Glu Lys Leu Ser Pro Asn Ser Gln Ser Arg Ser Gly Asp Gly Tyr
450 455 460
Lys Thr Ala Gly Glu Gly Thr Ala Lys Thr Pro Ser Ala Lys Asp Pro
465 470 475 480
Ser Thr Ala Asn Lys Ala Asn Lys
485
<210> 41
<211> 64
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 41
Ala Ser Leu Asn Gly Ser Leu Ile Leu Val Lys Trp Asn Ile Ile Arg
1 5 10 15
Ser Gly Ala Thr Ala Ala Asp Thr Phe Ile Ile Leu Ile Asn Thr Leu
20 25 30
Phe Thr Leu Gln Leu Asn Thr Gly Gly Phe Pro Phe Ser Cys Ser Gly
35 40 45
Leu Ile Ser Ser Leu Ala Ser Lys Lys Leu Glu Lys Ile Ser Ser Met
50 55 60
<210> 42
<211> 66
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 42
Leu Thr Lys Ser Tyr Leu Thr Asn Leu Glu Ser Ile Leu Val Phe Leu
1 5 10 15
Leu Leu Met Ser Leu Asn Gln Tyr Leu Lys Ser Phe Leu Gln Ile Ala
20 25 30
Lys Ala Ala Gln Glu Met Val Ile Arg Pro Leu Glu Lys Val Leu Gln
35 40 45
Arg His Leu Gln Arg Lys Thr Gln Ala Gln Gln Ile Arg Gln Ile Asn
50 55 60
Asn Gly
65
<210> 43
<211> 66
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 43
Thr Leu Ser Pro Pro Ile Glu Ser Thr Lys Val Ile Gln Leu Asn Ser
1 5 10 15
Ser Ala Gly Ile Leu Pro Phe Gly Asn Ile Glu Pro Phe Gln Leu Ser
20 25 30
Ile Thr Leu Ile Gly Asn Ser Leu Glu His Arg Lys Pro Thr Phe Asn
35 40 45
Tyr Leu Gln Ser Phe Leu Arg Ile Leu Thr Leu Leu Met Ser Ala Ala
50 55 60
Lys Met
65
<210> 44
<211> 450
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 44
Val Lys Met Ser Ser Asn Ile Phe Arg Leu Ala Asp Arg Leu Phe Asn
1 5 10 15
Gln Pro Leu Leu Ala Thr Glu Ser Leu Ala His Ser Ala Ala Thr Tyr
20 25 30
Val Asn Asn Arg Leu Leu Gly Asp Val Gln Ala Ala Val Asn Phe Asp
35 40 45
Lys Pro Lys Gly Glu Ala Arg Ser Leu Leu Lys Val Lys Asp Asp Ile
50 55 60
Ala Ile Ile Pro Ile Met Gly Gly Leu Thr His Arg Met Thr Phe Ile
65 70 75 80
Asp Ala Met Cys Thr Gly Gly Leu Ser Ser Tyr Glu Gly Leu Arg Arg
85 90 95
Gly Phe Asp Glu Ala Leu Ala Asp Glu Ser Ile Lys Thr Ile Val Leu
100 105 110
His Ile Asp Ser Gly Gly Gly Glu Ala Ser Gly Cys Phe Glu Leu Ala
115 120 125
Arg His Ile Met Ala Ser Arg Gly Gln Lys Lys Ile Ile Ala Tyr Val
130 135 140
Asp Glu Phe Ala Cys Ser Ala Ala Tyr Ala Leu Ala Ser Ser Ala Glu
145 150 155 160
Glu Ile Ile Ala Ser Pro Asp Ala Asp Val Gly Ser Ile Gly Val Ile
165 170 175
Met Val His Gln Glu Leu Thr Lys Ala Phe Glu Lys Asn Gly Val Thr
180 185 190
Ile Asn Val Ile Lys Ala Gly Glu Phe Lys Gly Met Gly Ser Pro Phe
195 200 205
Gln Ala Leu Ser Glu Glu Ser Lys Glu Arg Leu Gln Lys Arg Ile Asn
210 215 220
Asp Thr Tyr Ala Thr Phe Thr Gly Phe Val Ala Glu Ser Arg Asn Leu
225 230 235 240
Ser Glu Glu Ala Val Lys Asn Thr Glu Ala Asn Val Tyr Ser Ala Gln
245 250 255
Glu Ala Leu Glu Leu Gly Leu Ile Asn Ser Ile Met Ser Gln Asp Asp
260 265 270
Phe Leu Asn Tyr Leu Gln Gly Ser Glu Glu Ala Pro Val Ser Leu Asn
275 280 285
Val Asn Asn Ser Gly Glu Glu Met Thr Glu Gln Glu Lys Gln Glu Leu
290 295 300
Glu Ala Leu Arg Leu Gln Val Ala Gln Met Lys Ala Lys Glu Gln Glu
305 310 315 320
Ala Ala Leu Ser Asp Leu Thr Asn Lys Ile Ser Ala Ser Ala Glu Ala
325 330 335
Phe Gly Phe Asp Ala Lys Glu Ala Ala Thr Thr Ile Leu Gly Ala Gly
340 345 350
Leu Asp Asn Pro Leu Ser Val Leu Phe Met Asn Ala Met Glu Gly Ala
355 360 365
Asn Gln Lys Leu Asn Glu Thr Ile Ala Ser His Ala Ser Ala Met Glu
370 375 380
Glu Lys Glu Ser Glu Ile Thr Lys Leu Lys Glu Thr Ala Gly Ala Val
385 390 395 400
Leu Glu His Ser Asn Ala Met Glu Glu Met Gly Asn Asp Gly Glu Ala
405 410 415
Asp Leu Val Glu Glu Glu Lys Glu Pro Ala Lys Asn Ala Ser Glu Asp
420 425 430
Thr Ala Glu Gln Arg Lys Leu Ala Leu Gln Asn Ala Leu Lys Ser Leu
435 440 445
Ile Lys
450
<210> 45
<211> 448
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 45
Met Ser Ser Asn Ile Phe Arg Leu Ala Asp Arg Leu Phe Asn Gln Pro
1 5 10 15
Leu Leu Ala Thr Glu Ser Leu Ala His Ser Ala Ala Thr Tyr Val Asn
20 25 30
Asn Arg Leu Leu Gly Asp Val Gln Ala Ala Val Asn Phe Asp Lys Pro
35 40 45
Lys Gly Glu Ala Arg Ser Leu Leu Lys Val Lys Asp Asp Ile Ala Ile
50 55 60
Ile Pro Ile Met Gly Gly Leu Thr His Arg Met Thr Phe Ile Asp Ala
65 70 75 80
Met Cys Thr Gly Gly Leu Ser Ser Tyr Glu Gly Leu Arg Arg Gly Phe
85 90 95
Asp Glu Ala Leu Ala Asp Glu Ser Ile Lys Thr Ile Val Leu His Ile
100 105 110
Asp Ser Gly Gly Gly Glu Ala Ser Gly Cys Phe Glu Leu Ala Arg His
115 120 125
Ile Met Ala Ser Arg Gly Gln Lys Lys Ile Ile Ala Tyr Val Asp Glu
130 135 140
Phe Ala Cys Ser Ala Ala Tyr Ala Leu Ala Ser Ser Ala Glu Glu Ile
145 150 155 160
Ile Ala Ser Pro Asp Ala Asp Val Gly Ser Ile Gly Val Ile Met Val
165 170 175
His Gln Glu Leu Thr Lys Ala Phe Glu Lys Asn Gly Val Thr Ile Asn
180 185 190
Val Ile Lys Ala Gly Glu Phe Lys Gly Met Gly Ser Pro Phe Gln Ala
195 200 205
Leu Ser Glu Glu Ser Lys Glu Arg Leu Gln Lys Arg Ile Asn Asp Thr
210 215 220
Tyr Ala Thr Phe Thr Gly Phe Val Ala Glu Ser Arg Asn Leu Ser Glu
225 230 235 240
Glu Ala Val Lys Asn Thr Glu Ala Asn Val Tyr Ser Ala Gln Glu Ala
245 250 255
Leu Glu Leu Gly Leu Ile Asn Ser Ile Met Ser Gln Asp Asp Phe Leu
260 265 270
Asn Tyr Leu Gln Gly Ser Glu Glu Ala Pro Val Ser Leu Asn Val Asn
275 280 285
Asn Ser Gly Glu Glu Met Thr Glu Gln Glu Lys Gln Glu Leu Glu Ala
290 295 300
Leu Arg Leu Gln Val Ala Gln Met Lys Ala Lys Glu Gln Glu Ala Ala
305 310 315 320
Leu Ser Asp Leu Thr Asn Lys Ile Ser Ala Ser Ala Glu Ala Phe Gly
325 330 335
Phe Asp Ala Lys Glu Ala Ala Thr Thr Ile Leu Gly Ala Gly Leu Asp
340 345 350
Asn Pro Leu Ser Val Leu Phe Met Asn Ala Met Glu Gly Ala Asn Gln
355 360 365
Lys Leu Asn Glu Thr Ile Ala Ser His Ala Ser Ala Met Glu Glu Lys
370 375 380
Glu Ser Glu Ile Thr Lys Leu Lys Glu Thr Ala Gly Ala Val Leu Glu
385 390 395 400
His Ser Asn Ala Met Glu Glu Met Gly Asn Asp Gly Glu Ala Asp Leu
405 410 415
Val Glu Glu Glu Lys Glu Pro Ala Lys Asn Ala Ser Glu Asp Thr Ala
420 425 430
Glu Gln Arg Lys Leu Ala Leu Gln Asn Ala Leu Lys Ser Leu Ile Lys
435 440 445
<210> 46
<211> 125
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 46
Met Ala Tyr Gln Asp Phe Thr Lys Leu Gly Lys Arg Glu Pro Leu Asn
1 5 10 15
Asp Ile Ile Leu Trp Glu Gln Val Thr Pro Thr Gly His Ser Arg Lys
20 25 30
Glu Tyr Thr Pro Gln Ala Ser Thr Glu Tyr Arg Val Gly Glu Val Leu
35 40 45
Lys Glu Asp Gly Thr Lys Val Ala Ala Gly Glu Glu Ala Gln Ala Asp
50 55 60
Ser Val Cys Ile Val Asn Phe Tyr Ser Asp Leu Gln Leu Ser Tyr His
65 70 75 80
Gly Gln Leu Lys Val Val Gly Ile Tyr Arg Asp Ala Glu Leu Lys Asp
85 90 95
Met Leu Thr Leu Glu Ser Gly Val Asn Ala Glu Asn Val Lys Lys Ala
100 105 110
Leu Ala Ala Lys Gly Ile Asp Phe Val Pro Thr Gly Leu
115 120 125
<210> 47
<211> 150
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 47
Ile Phe Ala Leu Ser Cys Ser Ala Ala Lys Asp Ser Lys Val Leu Leu
1 5 10 15
Ile Glu Ala Glu Ala Ser Ala Lys Pro Asn Ser Ala Phe Ser Ala Ala
20 25 30
Val Val Ile Lys Pro Ala Pro Arg Ser Leu Gly Arg Leu Thr Arg Asn
35 40 45
Ile Phe Ala Ile Ser Pro Ala Leu Trp Phe Ser Leu Ser Val Ile Ala
50 55 60
Asp Trp Ala Glu Ala Ile Ser Ser Phe Ser Asp Ser Ile Val Leu Ser
65 70 75 80
Phe Ser Val Ala Pro Ala Thr Arg Ser Cys Glu Leu Ala Ile Ser Ser
85 90 95
Ile Pro Leu Ser Pro Ser Ala Ser Lys Thr Ser Ser Ser Phe Ser Gly
100 105 110
Ala Leu Phe Ala Glu Ser Ser Val Ala Ser Cys Arg Leu Ser Ala Arg
115 120 125
Trp Phe Ala Arg Phe Asp Arg Ile Leu Tyr Pro Val Cys Tyr His Cys
130 135 140
Ile Leu Ile Lys Ser Leu
145 150
<210> 48
<211> 374
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 48
Asp Ile Leu Glu Asn Phe Thr Met Leu Thr Asn Ser Glu Lys Ser Arg
1 5 10 15
Phe Phe Leu Ala Asp Leu Thr Gly Glu Val Gln Ser Ile Pro Asn Thr
20 25 30
Tyr Gly Tyr Ile Ser Asn Leu Gly Leu Phe Arg Ser Ala Pro Ile Thr
35 40 45
Gln Thr Thr Phe Leu Met Asp Leu Thr Asp Trp Asp Val Ser Leu Leu
50 55 60
Asp Ala Val Asp Arg Asp Ser Arg Lys Ala Glu Thr Ser Ala Pro Glu
65 70 75 80
Arg Val Arg Gln Ile Ser Phe Pro Met Met Tyr Phe Lys Glu Val Glu
85 90 95
Ser Ile Thr Pro Asp Glu Ile Gln Gly Val Arg Gln Pro Gly Thr Ala
100 105 110
Asn Glu Leu Thr Thr Glu Ala Val Val Arg Ala Lys Lys Leu Met Lys
115 120 125
Ile Arg Thr Lys Phe Asp Ile Thr Arg Glu Phe Leu Phe Met Gln Ala
130 135 140
Leu Lys Gly Lys Val Val Asp Ala Arg Gly Thr Leu Tyr Ala Asp Leu
145 150 155 160
Tyr Lys Gln Phe Asp Val Glu Lys Lys Thr Val Tyr Phe Asp Leu Gly
165 170 175
Asn Pro Asn Ala Asp Ile Asp Ala Ser Ile Glu Glu Leu Arg Met His
180 185 190
Met Glu Asp Glu Ala Lys Thr Gly Thr Val Ile Asn Gly Glu Glu Ile
195 200 205
His Val Val Val Asp Arg Val Phe Phe Ser Lys Leu Val Lys His Pro
210 215 220
Lys Ile Arg Asp Ala Tyr Leu Ala Gln Gln Thr Pro Leu Ala Trp Gln
225 230 235 240
Gln Ile Thr Gly Ser Leu Arg Thr Gly Gly Thr Asp Gly Val Gln Ala
245 250 255
His Met Asn Thr Phe Tyr Tyr Gly Gly Val Lys Phe Val Gln Tyr Asn
260 265 270
Gly Lys Phe Lys Asp Lys Arg Gly Lys Val His Thr Leu Val Ser Ile
275 280 285
Asp Gly Ala Gly Ala Glu Val Gly Val Gly His Ala Phe Pro Asn Val
290 295 300
Ser Met Leu Gly Glu Ala Asn Asn Ile Phe Glu Val Ala Tyr Gly Pro
305 310 315 320
Cys Pro Lys Met Gly Tyr Ala Asn Thr Leu Gly Gln Glu Leu Tyr Val
325 330 335
Phe Glu Tyr Glu Lys Asp Arg Asp Glu Gly Ile Asp Phe Glu Ala His
340 345 350
Ser Tyr Met Leu Pro Tyr Cys Thr Arg Pro Gln Leu Leu Val Asp Val
355 360 365
Arg Ala Asp Ala Lys Asp
370
<210> 49
<211> 372
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 49
Leu Glu Asn Phe Thr Met Leu Thr Asn Ser Glu Lys Ser Arg Phe Phe
1 5 10 15
Leu Ala Asp Leu Thr Gly Glu Val Gln Ser Ile Pro Asn Thr Tyr Gly
20 25 30
Tyr Ile Ser Asn Leu Gly Leu Phe Arg Ser Ala Pro Ile Thr Gln Thr
35 40 45
Thr Phe Leu Met Asp Leu Thr Asp Trp Asp Val Ser Leu Leu Asp Ala
50 55 60
Val Asp Arg Asp Ser Arg Lys Ala Glu Thr Ser Ala Pro Glu Arg Val
65 70 75 80
Arg Gln Ile Ser Phe Pro Met Met Tyr Phe Lys Glu Val Glu Ser Ile
85 90 95
Thr Pro Asp Glu Ile Gln Gly Val Arg Gln Pro Gly Thr Ala Asn Glu
100 105 110
Leu Thr Thr Glu Ala Val Val Arg Ala Lys Lys Leu Met Lys Ile Arg
115 120 125
Thr Lys Phe Asp Ile Thr Arg Glu Phe Leu Phe Met Gln Ala Leu Lys
130 135 140
Gly Lys Val Val Asp Ala Arg Gly Thr Leu Tyr Ala Asp Leu Tyr Lys
145 150 155 160
Gln Phe Asp Val Glu Lys Lys Thr Val Tyr Phe Asp Leu Gly Asn Pro
165 170 175
Asn Ala Asp Ile Asp Ala Ser Ile Glu Glu Leu Arg Met His Met Glu
180 185 190
Asp Glu Ala Lys Thr Gly Thr Val Ile Asn Gly Glu Glu Ile His Val
195 200 205
Val Val Asp Arg Val Phe Phe Ser Lys Leu Val Lys His Pro Lys Ile
210 215 220
Arg Asp Ala Tyr Leu Ala Gln Gln Thr Pro Leu Ala Trp Gln Gln Ile
225 230 235 240
Thr Gly Ser Leu Arg Thr Gly Gly Thr Asp Gly Val Gln Ala His Met
245 250 255
Asn Thr Phe Tyr Tyr Gly Gly Val Lys Phe Val Gln Tyr Asn Gly Lys
260 265 270
Phe Lys Asp Lys Arg Gly Lys Val His Thr Leu Val Ser Ile Asp Gly
275 280 285
Ala Gly Ala Glu Val Gly Val Gly His Ala Phe Pro Asn Val Ser Met
290 295 300
Leu Gly Glu Ala Asn Asn Ile Phe Glu Val Ala Tyr Gly Pro Cys Pro
305 310 315 320
Lys Met Gly Tyr Ala Asn Thr Leu Gly Gln Glu Leu Tyr Val Phe Glu
325 330 335
Tyr Glu Lys Asp Arg Asp Glu Gly Ile Asp Phe Glu Ala His Ser Tyr
340 345 350
Met Leu Pro Tyr Cys Thr Arg Pro Gln Leu Leu Val Asp Val Arg Ala
355 360 365
Asp Ala Lys Asp
370
<210> 50
<211> 149
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 50
Met Cys Tyr Thr Gly Asp Pro Ala Asn Asn Pro Leu Asp Arg Val Arg
1 5 10 15
Ile Leu Cys Thr Asp Thr Asn Asn Asp Glu Ile Leu Ile Glu Gln Ser
20 25 30
Val Leu Glu Trp Phe Tyr Leu Glu Ser Gly Lys Asp Glu Lys Lys Ala
35 40 45
Ala Ile Lys Ala Leu Lys Tyr Leu Leu Phe Gln Val Ala Lys Met Gly
50 55 60
Asp Glu Lys Val Gly Gly Val Tyr Leu Arg Asn Ser Ser Arg Phe Lys
65 70 75 80
Ser Leu Lys Ala Val Tyr Asp Asp Leu Val Lys Ser Ser Val Ser Gly
85 90 95
Leu Pro Tyr Ala Gly Gly Ile Asn Gln Cys Asp Ile Asp Met Arg Arg
100 105 110
Gln Asn Pro Cys Ser Val Lys Lys Tyr Thr Glu Tyr Gly Asp Ala Ala
115 120 125
Arg Tyr Glu Gly Arg Asp Tyr Cys Asn Arg Val Asn Gly Val Phe Ile
130 135 140
Ile Glu Arg Asp Glu
145
<210> 51
<211> 68
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 51
Leu Phe Met Thr Thr Leu Leu Lys Ala Leu Phe Gln Asp Tyr Pro Met
1 5 10 15
Gln Val Val Leu Ile Ser Val Thr Leu Ile Cys Val Val Arg Ile Leu
20 25 30
Ala Leu Ser Arg Asn Thr Gln Asn Met Val Met Leu Pro Asp Thr Lys
35 40 45
Ala Glu Ile Thr Ala Thr Val Leu Met Ala Tyr Leu Leu Ser Ser Glu
50 55 60
Met Asn Asn Gly
65
<210> 52
<211> 133
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 52
Met Arg Leu Leu Asn Arg His Ser Phe Val Val Lys Arg Lys Val Ser
1 5 10 15
Glu Asp Gly Tyr Tyr Asn Asp Asp Gly Asp Trp Val Ala Ser Gln Asp
20 25 30
Ile Val Glu Val Asn Cys Lys Gly Asn Ile Gln Pro Tyr Ile Lys Gly
35 40 45
Ser Val Lys Asn Gly Thr Gln Ile Ala Leu Pro Glu Gly Ile Arg Leu
50 55 60
Thr Asp Thr Arg Ile Leu Tyr Thr Thr Tyr Lys Leu Arg Thr Ser Asp
65 70 75 80
Asp Val Glu Trp Asn Glu Ser Asp Ile Val Met Ile Asp Gly His Glu
85 90 95
Tyr Glu Val Phe Met Thr Met Asp Trp Ser Gln Gln Leu Ala His Thr
100 105 110
Ser His Tyr Glu Tyr Ile Ile Ile Arg Arg Asp Lys Met Asn Ala Val
115 120 125
Arg Asn Ser Arg Thr
130
<210> 53
<211> 450
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 53
Met Trp Asn Pro Ile Val Asn Val Asp Ile Thr Leu Asn Thr Ala Gly
1 5 10 15
Thr Thr Arg Glu Gly Phe Gly Leu Pro Leu Phe Leu Ala Ser Thr Asp
20 25 30
Asn Phe Glu Glu Arg Val Arg Gly Tyr Thr Ser Leu Thr Glu Val Ala
35 40 45
Glu Asp Phe Asp Glu Asn Thr Ala Ala Tyr Lys Ala Ala Lys Gln Leu
50 55 60
Trp Ser Gln Thr Pro Lys Val Thr Gln Leu Tyr Ile Gly Arg Cys Ala
65 70 75 80
Met Gln Tyr Thr Val Ser Ile Pro Asp Glu Val Thr Glu Ser Thr Asp
85 90 95
Tyr Ser Ile Thr Val Ala Val Gly Gly Gly Met Ser Gln Pro Phe Gln
100 105 110
Tyr Thr Ser Gln Gly Glu Asp Thr Ala Glu Asn Val Leu Gln Gln Phe
115 120 125
Lys Thr Gln Ile Glu Ala Asp Gln Ser Ile Lys Asp Lys Val Ser Val
130 135 140
Asn Val Thr Gly Ser Asn Gly Ser Ala Thr Met Val Ile Ser Lys Ala
145 150 155 160
Gly Asp Asn Asp Phe Val Lys Val Thr Thr Thr Ala Gln Thr Val Ser
165 170 175
Ile Ala Ser Thr Thr Ala Asp Thr Ala Ser Thr Ala Leu Ala Ala Ile
180 185 190
Glu Ala Tyr Ser Thr Asp Trp Tyr Phe Ile Ala Ala Glu Asp Arg Thr
195 200 205
Gln Gln Phe Val Leu Ala Met Ala Ser Glu Ile Gln Ala Arg Lys Lys
210 215 220
Ile Phe Phe Thr Ala Asn Ser Asp Val Ala Ala Leu Gln Gly Thr Glu
225 230 235 240
Leu Asn Ser Ala Asn Asp Val Pro Ala Gln Leu Ala Lys Ser Met Tyr
245 250 255
Thr Arg Thr Val Cys Leu Trp His His Thr Ala Ala Glu Asp Tyr Pro
260 265 270
Glu Met Ala Tyr Ile Ala Tyr Gly Ala Pro Tyr Asp Ala Gly Ser Ile
275 280 285
Ala Trp Gly Asn Ala Gln Leu Thr Gly Val Ala Ala Ser Leu Gln Pro
290 295 300
Ala Asn Lys Arg Pro Leu Thr Ser Ile Gln Lys Ser Ala Leu Asp Val
305 310 315 320
Arg His Cys Asn Phe Ile Asp Leu Asp Gly Gly Val Pro Val Val Arg
325 330 335
Arg Gly Ile Thr Ser Gly Gly Glu Trp Ile Asp Ile Val Arg Gly Val
340 345 350
Asp Trp Leu Glu Ser Asp Leu Lys Thr Ser Leu Arg Asp Leu Leu Ile
355 360 365
Asn Gln Lys Gly Gly Lys Ile Thr Tyr Asp Asp Thr Gly Ile Thr Arg
370 375 380
Ile Arg Gln Val Ile Glu Thr Ser Leu Gln Arg Ala Val Asn Arg Asn
385 390 395 400
Phe Leu Ser Ser Tyr Thr Val Asn Val Pro Lys Ala Ser Gln Val Ala
405 410 415
Leu Ala Asp Lys Lys Ala Arg Ile Leu Lys Asp Val Thr Phe Ala Gly
420 425 430
Ile Leu Ala Gly Ala Ile Leu Asp Val Asp Leu Lys Gly Thr Val Ala
435 440 445
Tyr Glu
450
<210> 54
<211> 150
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 54
Ile Gly Met Ala Met Tyr Gln Gln Tyr Ser Pro Lys Asp Val Val Cys
1 5 10 15
Ser Trp Asn Gly Ile Ala Ile Glu Gly Phe Ala Pro Asp Ser Phe Leu
20 25 30
Arg Leu Gln Arg Thr Ser Pro Leu Ile Thr Pro Val Val Gly Ala Gly
35 40 45
Gly Gln Val Ala Leu Thr Arg Asn Ala Asp Lys Thr Gly Thr Ile Glu
50 55 60
Ile Glu Leu Met Gln Thr Ser Leu Ser Asn Gln Met Leu Ser Ala Ile
65 70 75 80
Gln Ala Lys Gln Asp Asn Met Glu Leu Glu Glu Asp Ile Ser Ser Asn
85 90 95
Phe Val Ile Tyr Asp Pro Ser Gly Ser Val Leu Ala Thr Gly Ile Asn
100 105 110
Ala Trp Leu Gln Glu Leu Pro Gln Ile Glu Leu Gly Arg Asp Gln Asn
115 120 125
Ser Lys Thr Trp Ile Phe Gly Cys Glu Lys Leu Asp Tyr Thr Ser Thr
130 135 140
Ile Pro Ala Ser Ser Val
145 150
<210> 55
<211> 148
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 55
Met Ala Met Tyr Gln Gln Tyr Ser Pro Lys Asp Val Val Cys Ser Trp
1 5 10 15
Asn Gly Ile Ala Ile Glu Gly Phe Ala Pro Asp Ser Phe Leu Arg Leu
20 25 30
Gln Arg Thr Ser Pro Leu Ile Thr Pro Val Val Gly Ala Gly Gly Gln
35 40 45
Val Ala Leu Thr Arg Asn Ala Asp Lys Thr Gly Thr Ile Glu Ile Glu
50 55 60
Leu Met Gln Thr Ser Leu Ser Asn Gln Met Leu Ser Ala Ile Gln Ala
65 70 75 80
Lys Gln Asp Asn Met Glu Leu Glu Glu Asp Ile Ser Ser Asn Phe Val
85 90 95
Ile Tyr Asp Pro Ser Gly Ser Val Leu Ala Thr Gly Ile Asn Ala Trp
100 105 110
Leu Gln Glu Leu Pro Gln Ile Glu Leu Gly Arg Asp Gln Asn Ser Lys
115 120 125
Thr Trp Ile Phe Gly Cys Glu Lys Leu Asp Tyr Thr Ser Thr Ile Pro
130 135 140
Ala Ser Ser Val
145
<210> 56
<211> 106
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 56
Leu Leu Ser Ile Cys Ala Gly Gly Lys Leu Arg Lys Phe Phe Arg Ser
1 5 10 15
Lys His Phe Gln Lys Pro Asn Phe Ser Val Asn Met Gly Asn Thr Leu
20 25 30
Gln Thr Pro Leu Thr Asp Ala Ala Val Glu Ala Thr Tyr Glu Glu Ala
35 40 45
Asp Glu Met Lys Phe Val Leu Ala Ile Tyr Gly Met Glu Gly Cys Lys
50 55 60
Glu Thr Leu Asp Gln Leu Phe Ala Met Thr Phe Ser Asp Leu Leu Ser
65 70 75 80
Leu Arg Gln Phe Leu Glu Ile Gln Arg Ser Tyr Lys Glu Glu Ile Ala
85 90 95
Tyr Asn Glu Leu Arg Arg Ala Gly Lys Met
100 105
<210> 57
<211> 746
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 57
Arg Lys Asn Val Met Ala Gln Tyr Thr Val Asp Ser Phe Ile Val Glu
1 5 10 15
Leu Gly Phe Ser Glu Lys Val Val Lys Gly Leu Gln Arg Val Glu Lys
20 25 30
Met Ser Met Gln Ala Ala Gln Arg Ile Glu Arg Asn Ile Asn Lys Ala
35 40 45
Phe Asp Val Lys Pro Asn Lys Ser Ser Gln Glu Ala Leu Asn Arg Ile
50 55 60
Val Lys Asn Ala Gln Ser Ala Ser Gly Arg Ile Asn Lys Ala Leu Asn
65 70 75 80
Ser Ser Leu Asn Leu Asp Ser Gln Gly Val Lys Ser Leu Lys Lys Leu
85 90 95
Glu Thr Gln Ala Lys Lys Thr Ala Lys Gly Ile Asn Lys Ser Leu Arg
100 105 110
Asp Ala Met Lys Val Asp Gly Lys Ile Thr Ile Lys Thr Gly Arg Gly
115 120 125
Arg Gly Gly Gln Ser Ile Pro Pro Val Gly Gly Gly Ala Pro Arg Gly
130 135 140
Pro Arg Val Asp Val Ala Gln Arg Gln Met Glu Arg Met Phe Asn Asn
145 150 155 160
Asn Phe Tyr Ser Gly Leu Thr Arg Arg Leu Glu Thr Ile Gly Gly Gln
165 170 175
Gly Asn Gln Met Ala Ala Ser Phe Arg Gly Ser Leu Gln Asn Ile Tyr
180 185 190
Asn Arg Tyr Lys Gly Thr Gly Lys Val Gly Glu Tyr Glu Met Glu Val
195 200 205
Lys Lys Leu Ile Asp Val Thr Lys Arg Trp Val Ile Ala Glu Asn Ala
210 215 220
Arg Leu Lys Ser Val Lys Glu Ser Ala Trp Leu Gln Asp Arg Ala Asn
225 230 235 240
Ala Ser Leu Arg Gln Leu Val Gly Gly Phe Val Ser Ala Tyr Ala Leu
245 250 255
Leu Glu Leu Ser Gln Lys Thr Ile Glu Ala Gly Val Lys Arg Gln Ser
260 265 270
Ala Gln Leu Ala Ser Thr Ala Ile Phe Gly Ala Asp Thr Gln Gln Ala
275 280 285
Arg Met Phe Ala Ala Ser Phe Ala His Gln Ile Gly Gln Asn Tyr Thr
290 295 300
Asp Thr Met Lys Gln Tyr Ser Asn Phe Ala Ala Gly Ala Gln Pro Thr
305 310 315 320
Leu Gly Phe Gln Gly Thr Gln Glu Phe Tyr Lys Asn Ala Ala Met Phe
325 330 335
Ala Arg Ile Arg Gly Ala Thr Asp Glu Asp Leu Lys Gly Ile Met Val
340 345 350
Ala Phe Gln Gln Met Ala Ser Lys Gly Lys Val Gln Ala Glu Glu Leu
355 360 365
Arg Gly Gln Leu Gly Asp Arg Leu Ala Gly Ala Val Gln Leu Phe Ala
370 375 380
Asp Ala Ile Gly Lys Thr Pro Gln Glu Leu Asp Lys Met Met Gln Asp
385 390 395 400
Gly Lys Leu Leu Ala Gln Asp Val Leu Pro Lys Val Ser Glu Arg Met
405 410 415
Ala Glu Leu Val Lys Gln Ala Gly Gly Met Asn Ala Val Ser Lys Gln
420 425 430
Thr Ala Thr Ser Met Gly Gln Ala Lys Ala Met Trp Asp Asn Thr Leu
435 440 445
Val Ala Leu Phe Asn Asn Ser Ser Glu Gly Ile Ser Gln Leu Ser Asn
450 455 460
Ser Val Ala Met Phe Leu Gln Gly Ser Met Gly Ser Thr Gln Ala Leu
465 470 475 480
Gly Leu Val Ile Gly Asn Leu Leu Lys Gly Ala Ser Asn Leu Leu Asp
485 490 495
Phe Ile Thr Asp Phe Met Tyr Arg Thr Ser Ala Leu Tyr Tyr Tyr Ala
500 505 510
Arg Ala Trp Tyr Lys Asp Leu Asp Asn Ser Gln Gln Lys Leu Ile Lys
515 520 525
Ser Ala Gly Glu Phe Leu Gly Thr Val Val Thr Ile Gly Gly Ala Val
530 535 540
Ala Val Val Ser Lys Ala Val Lys Leu Leu Ser Gly Leu Val Gly Gly
545 550 555 560
Gly Ile Phe Gly Lys Ile Leu Gln Arg Leu Gly Val Ser Ala Ala Gly
565 570 575
Thr Ala Ala Ala Gly Glu Ala Ala Ala Ala Ala Gly Gly Val Thr Ala
580 585 590
Thr Arg Met Ala Leu Gly Thr Val Gly Ser Ala Leu Met Leu Arg Gly
595 600 605
Ser Thr Asp Pro Asn Ala Ala Lys Asn Tyr Ser Glu Val Thr Leu Pro
610 615 620
Lys Pro Phe Glu Asn Ala Val Ala Asn Ile Thr Asn Pro Lys Arg Pro
625 630 635 640
Met Phe Phe Asp Glu Asn Gly Gln Leu Gln Phe Ala Gln Tyr Thr Gln
645 650 655
Asp Val Glu Gly Asn Arg Lys Leu Ile Asp Asn Gly Leu Ser Asn Trp
660 665 670
Glu Ile Ile Met Glu Lys Leu Ser Thr Ser Ile Asp Asn Phe Ala Asn
675 680 685
Lys Phe Asn Gln Thr Pro Met Met Met Thr Pro Ser Gly Leu Pro Met
690 695 700
Gln Thr Lys Gln Thr Leu Asn Val Thr Phe Asn Leu Asp Gly Lys Gln
705 710 715 720
Ile Ala Thr Lys Met Val Asp Ile Thr Asp Lys Asn Gln Glu Asp Ile
725 730 735
Leu Leu Ser Ser Ser Tyr Pro Glu Glu Glu
740 745
<210> 58
<211> 742
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 58
Met Ala Gln Tyr Thr Val Asp Ser Phe Ile Val Glu Leu Gly Phe Ser
1 5 10 15
Glu Lys Val Val Lys Gly Leu Gln Arg Val Glu Lys Met Ser Met Gln
20 25 30
Ala Ala Gln Arg Ile Glu Arg Asn Ile Asn Lys Ala Phe Asp Val Lys
35 40 45
Pro Asn Lys Ser Ser Gln Glu Ala Leu Asn Arg Ile Val Lys Asn Ala
50 55 60
Gln Ser Ala Ser Gly Arg Ile Asn Lys Ala Leu Asn Ser Ser Leu Asn
65 70 75 80
Leu Asp Ser Gln Gly Val Lys Ser Leu Lys Lys Leu Glu Thr Gln Ala
85 90 95
Lys Lys Thr Ala Lys Gly Ile Asn Lys Ser Leu Arg Asp Ala Met Lys
100 105 110
Val Asp Gly Lys Ile Thr Ile Lys Thr Gly Arg Gly Arg Gly Gly Gln
115 120 125
Ser Ile Pro Pro Val Gly Gly Gly Ala Pro Arg Gly Pro Arg Val Asp
130 135 140
Val Ala Gln Arg Gln Met Glu Arg Met Phe Asn Asn Asn Phe Tyr Ser
145 150 155 160
Gly Leu Thr Arg Arg Leu Glu Thr Ile Gly Gly Gln Gly Asn Gln Met
165 170 175
Ala Ala Ser Phe Arg Gly Ser Leu Gln Asn Ile Tyr Asn Arg Tyr Lys
180 185 190
Gly Thr Gly Lys Val Gly Glu Tyr Glu Met Glu Val Lys Lys Leu Ile
195 200 205
Asp Val Thr Lys Arg Trp Val Ile Ala Glu Asn Ala Arg Leu Lys Ser
210 215 220
Val Lys Glu Ser Ala Trp Leu Gln Asp Arg Ala Asn Ala Ser Leu Arg
225 230 235 240
Gln Leu Val Gly Gly Phe Val Ser Ala Tyr Ala Leu Leu Glu Leu Ser
245 250 255
Gln Lys Thr Ile Glu Ala Gly Val Lys Arg Gln Ser Ala Gln Leu Ala
260 265 270
Ser Thr Ala Ile Phe Gly Ala Asp Thr Gln Gln Ala Arg Met Phe Ala
275 280 285
Ala Ser Phe Ala His Gln Ile Gly Gln Asn Tyr Thr Asp Thr Met Lys
290 295 300
Gln Tyr Ser Asn Phe Ala Ala Gly Ala Gln Pro Thr Leu Gly Phe Gln
305 310 315 320
Gly Thr Gln Glu Phe Tyr Lys Asn Ala Ala Met Phe Ala Arg Ile Arg
325 330 335
Gly Ala Thr Asp Glu Asp Leu Lys Gly Ile Met Val Ala Phe Gln Gln
340 345 350
Met Ala Ser Lys Gly Lys Val Gln Ala Glu Glu Leu Arg Gly Gln Leu
355 360 365
Gly Asp Arg Leu Ala Gly Ala Val Gln Leu Phe Ala Asp Ala Ile Gly
370 375 380
Lys Thr Pro Gln Glu Leu Asp Lys Met Met Gln Asp Gly Lys Leu Leu
385 390 395 400
Ala Gln Asp Val Leu Pro Lys Val Ser Glu Arg Met Ala Glu Leu Val
405 410 415
Lys Gln Ala Gly Gly Met Asn Ala Val Ser Lys Gln Thr Ala Thr Ser
420 425 430
Met Gly Gln Ala Lys Ala Met Trp Asp Asn Thr Leu Val Ala Leu Phe
435 440 445
Asn Asn Ser Ser Glu Gly Ile Ser Gln Leu Ser Asn Ser Val Ala Met
450 455 460
Phe Leu Gln Gly Ser Met Gly Ser Thr Gln Ala Leu Gly Leu Val Ile
465 470 475 480
Gly Asn Leu Leu Lys Gly Ala Ser Asn Leu Leu Asp Phe Ile Thr Asp
485 490 495
Phe Met Tyr Arg Thr Ser Ala Leu Tyr Tyr Tyr Ala Arg Ala Trp Tyr
500 505 510
Lys Asp Leu Asp Asn Ser Gln Gln Lys Leu Ile Lys Ser Ala Gly Glu
515 520 525
Phe Leu Gly Thr Val Val Thr Ile Gly Gly Ala Val Ala Val Val Ser
530 535 540
Lys Ala Val Lys Leu Leu Ser Gly Leu Val Gly Gly Gly Ile Phe Gly
545 550 555 560
Lys Ile Leu Gln Arg Leu Gly Val Ser Ala Ala Gly Thr Ala Ala Ala
565 570 575
Gly Glu Ala Ala Ala Ala Ala Gly Gly Val Thr Ala Thr Arg Met Ala
580 585 590
Leu Gly Thr Val Gly Ser Ala Leu Met Leu Arg Gly Ser Thr Asp Pro
595 600 605
Asn Ala Ala Lys Asn Tyr Ser Glu Val Thr Leu Pro Lys Pro Phe Glu
610 615 620
Asn Ala Val Ala Asn Ile Thr Asn Pro Lys Arg Pro Met Phe Phe Asp
625 630 635 640
Glu Asn Gly Gln Leu Gln Phe Ala Gln Tyr Thr Gln Asp Val Glu Gly
645 650 655
Asn Arg Lys Leu Ile Asp Asn Gly Leu Ser Asn Trp Glu Ile Ile Met
660 665 670
Glu Lys Leu Ser Thr Ser Ile Asp Asn Phe Ala Asn Lys Phe Asn Gln
675 680 685
Thr Pro Met Met Met Thr Pro Ser Gly Leu Pro Met Gln Thr Lys Gln
690 695 700
Thr Leu Asn Val Thr Phe Asn Leu Asp Gly Lys Gln Ile Ala Thr Lys
705 710 715 720
Met Val Asp Ile Thr Asp Lys Asn Gln Glu Asp Ile Leu Leu Ser Ser
725 730 735
Ser Tyr Pro Glu Glu Glu
740
<210> 59
<211> 65
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 59
Ala Phe Ala Ile His Ser Leu Val Ser Thr Ala Ser Asn Leu Leu Glu
1 5 10 15
Leu Ser Pro Met Glu Cys Asn Asp Leu Glu Thr Ala Ile Asn Lys Cys
20 25 30
Pro Glu Ile Pro Leu Val Cys Ala Lys Pro Arg Thr Ile Pro Phe Arg
35 40 45
Asn Phe Pro Ala Leu Leu Arg Ser Ser Lys Met Val Ser Lys Met Tyr
50 55 60
Leu
65
<210> 60
<211> 103
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 60
Gln Tyr Leu Ser Arg Ser Leu Leu Trp Cys Phe Ser Ile Leu Leu Ala
1 5 10 15
Pro Ser Asn Arg Pro Val Thr Thr Val Ile Pro Pro Ala Thr Ala Thr
20 25 30
Thr Asp Phe Ala Thr Leu Ser Arg Leu Pro Lys Thr Pro Pro Pro Ile
35 40 45
Lys Pro Leu Ile Lys Cys Leu Ser Pro Thr Leu Ala Ala Pro Val Ala
50 55 60
Ala Ala Pro Ser Ala Ala Ala Ala Ala Pro Pro Thr Val Ala Val Leu
65 70 75 80
Ile Ala Ser Pro Val Thr Pro Glu Ala Asn Ile Ser Leu Pro Glu Val
85 90 95
Ser Gly Phe Ala Ala Leu Phe
100
<210> 61
<211> 75
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 61
Ser Thr Ser Pro Leu Leu Phe Asn Ile Ser Leu Pro Arg Asp Leu Gln
1 5 10 15
Ser Ile Ile Met Ser Phe Ser Asp Val Asp Ile Ser Leu Lys Ala Leu
20 25 30
Leu Asn Leu Trp Val Gly Ile Ile Ile Val Gly Glu Pro Lys Gly Ile
35 40 45
Cys Val Leu Cys Val Arg Phe Thr Val Lys Leu Arg Ser Pro Leu Cys
50 55 60
Ile Ala Val Leu Ile Thr Ser Ile Val Ser Leu
65 70 75
<210> 62
<211> 113
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 62
Met Ser Gln Tyr Ile Pro Val Pro Asp Thr Gly Trp Ser Thr Gln Thr
1 5 10 15
Val Thr Leu Asp Gly Thr Val Phe Val Ile Glu Leu Lys Tyr Lys Glu
20 25 30
Arg Leu Asp Arg Trp Phe Leu Thr Leu Ser Asp Val Asp Gly Asn Val
35 40 45
Leu Leu Ser Glu Lys Lys Cys Leu Ala Asp Gln Ser Ile Thr Gly Arg
50 55 60
Tyr Val Ile Pro Ser Leu Ala Gly Glu Leu Phe Val Glu Arg Met Tyr
65 70 75 80
Gly Thr Asp Leu Gln Pro Thr Arg Asn Asn Phe Gly Arg Glu Lys Ala
85 90 95
Phe Glu Leu Asn Tyr Tyr Thr Gln Glu Asp Met Arg Leu Met Glu Asn
100 105 110
Leu
<210> 63
<211> 139
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 63
Met Lys Thr Asp Phe Ala Leu Asn Leu Gly Gly Asp Tyr Val Ala Thr
1 5 10 15
Leu Gly Ser Asp Ser Val Tyr Val Ala His Gly Asp Leu Lys Ile Thr
20 25 30
Gly Asn Gln Ile Arg Ile Ile Pro Glu Asp Asp Lys Ala Thr Gln Val
35 40 45
Ala Gln Arg Leu His Ile Arg Cys Leu Leu Arg Ala Gly Glu Val Phe
50 55 60
Phe Asn Thr Ser Ala Gly Phe Pro Tyr Leu Gln Leu Ala Lys Phe Lys
65 70 75 80
Gln Lys Thr Ser Ile Phe Asp Asn Tyr Met Lys Ala Tyr Leu Val Glu
85 90 95
Thr Arg Asp Val Ser Asn Ile Tyr Asn Tyr Ser Ser Ser Met Asp Asn
100 105 110
Ala Gln Arg Lys Val Thr Val Asn Phe Asp Ala Thr Thr Thr Thr Asp
115 120 125
Ile Leu Thr Asp Ile Thr Gln Glu Val Asn Ile
130 135
<210> 64
<211> 489
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 64
Met Ala Gly Leu Thr Thr Thr Gly Leu Gln Thr Leu Arg Tyr Gln Glu
1 5 10 15
Ile Phe Asp Asn Ile Lys Ser Arg Leu Leu Arg Asp Ile Ser Pro Asn
20 25 30
Leu Asp Val Ser Glu Asp Ser Gln Leu Gly Leu Phe Leu Ala Ser Ile
35 40 45
Ala Arg Ser Leu Ala Asp Thr His Glu Ile Leu Ser Glu Ile Tyr Asp
50 55 60
Gly Gly Thr Ile Asp Lys Ala Glu Gly Phe Asn Leu Asp Asp Ile Thr
65 70 75 80
Ala Leu Asn Ala Val Tyr Arg Tyr Val Ala Gln Ala Thr Arg Gly Gln
85 90 95
Val Glu Phe Thr Gly Thr Thr Gly Ala Thr Ile Pro Ser Thr Thr Arg
100 105 110
Leu Arg Ser Thr Ala Gly Asn Ile Phe Tyr Pro Val Ser Asn Ile Thr
115 120 125
Leu Thr Pro Ser Tyr Cys Val Glu Ala Ile Leu Glu Val Asn Ser Leu
130 135 140
Arg Thr Asp Ala Asn Tyr Val Ile Ile Ile Asp Asn Val Ile Phe Ser
145 150 155 160
Tyr Gln Pro Lys Ser Ser Asp Thr Ile Thr Val Leu Leu Thr Glu Leu
165 170 175
Ala Asn Ala Ile Asn Gly Gly Ile Val Ala Lys Ala Glu Val Ile Asn
180 185 190
Asp Gly Ser Ala Leu Arg Val Tyr Lys Asp Glu Gly Asp Ile Ile Ala
195 200 205
Arg Thr Asn Pro Met Val Val Thr Ala Thr Thr Phe Leu Thr Phe Thr
210 215 220
Lys Ile Thr Thr Ile His Asp Val Val Ala Glu Glu Val Gly Ala Ile
225 230 235 240
Pro Ala Leu Ala Gly Thr Leu Ile Glu Ile Glu Thr Thr Val Asp Gly
245 250 255
Leu Asp Ser Val Tyr Asn Arg Tyr Gly Leu Thr Thr Gly Arg Asn Glu
260 265 270
Glu Thr Asp Thr Glu Leu Arg Gln Arg Tyr Leu Glu Ser Leu Ser Val
275 280 285
Thr Gly Val Gly Thr Leu Asp Ala Ile Val Ala Ala Val Lys Arg Val
290 295 300
Gln Gly Val Ser Asp Ala Ser Gly Val Glu Asn Asp Thr Glu Glu Thr
305 310 315 320
Ser Ser Glu Gly Leu Pro Pro Lys Ser Phe Lys Ile Val Val Val Gly
325 330 335
Gly Gln Asn Asp Asn Val Ala Gln Ala Ile Trp Asp Thr Lys Pro Ala
340 345 350
Gly Ile Arg Ala Tyr Gly Ala Ile Phe Gly Thr Ala Tyr Asp Leu Gly
355 360 365
Gly Leu Ser His Asn Ile Tyr Phe Ser Arg Pro Thr Pro Lys Tyr Ala
370 375 380
Phe Val Lys Val Ser Tyr Ser Leu Tyr Asn Glu Glu Ser Leu Thr Ile
385 390 395 400
Pro Glu Glu Asp Ile Arg Asp Ser Ile Ile Gln Gly Ile Asn Ala Tyr
405 410 415
Gly Arg Thr Leu Lys Val Gly Asn Asp Val Ile Pro Asn Arg Ile Tyr
420 425 430
Gly Tyr Ile Tyr Asn Val Ile Lys Gly Ile Glu Ile Asn Glu Ile Lys
435 440 445
Val Ala Leu Ser Asn Ser Gln Ser Val Pro Pro Ser Asp Gly Gln Tyr
450 455 460
Thr Thr Ala Arg Ile Thr Val Asp Gly Asp Gln Tyr Thr Val Trp Glu
465 470 475 480
Ser Ser Lys Tyr Thr Ile Thr Lys Glu
485
<210> 65
<211> 489
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 65
Met Ala Gly Leu Thr Thr Thr Gly Leu Gln Thr Leu Arg Tyr Gln Glu
1 5 10 15
Ile Phe Asp Asn Ile Lys Ser Arg Leu Leu Arg Asp Ile Ser Pro Asn
20 25 30
Leu Asp Val Ser Glu Asp Ser Gln Leu Gly Leu Phe Leu Ala Ser Ile
35 40 45
Ala Arg Ser Leu Ala Asp Thr His Glu Ile Leu Ser Glu Ile Tyr Asp
50 55 60
Gly Gly Thr Ile Asp Lys Ala Glu Gly Phe Asn Leu Asp Asp Ile Thr
65 70 75 80
Ala Leu Asn Ala Val Tyr Arg Tyr Val Ala Gln Ala Thr Arg Gly Gln
85 90 95
Val Glu Phe Thr Gly Thr Thr Gly Ala Thr Ile Pro Ser Thr Thr Arg
100 105 110
Leu Arg Ser Thr Ala Gly Asn Ile Phe Tyr Pro Val Ser Asn Ile Thr
115 120 125
Leu Thr Pro Ser Tyr Cys Val Glu Ala Ile Leu Glu Val Asn Ser Leu
130 135 140
Arg Thr Asp Ala Asn Tyr Val Ile Ile Ile Asp Asn Val Ile Phe Ser
145 150 155 160
Tyr Gln Pro Lys Ser Ser Asp Thr Ile Thr Val Leu Leu Thr Glu Leu
165 170 175
Ala Asn Ala Ile Asn Gly Gly Ile Val Ala Lys Ala Glu Val Ile Asn
180 185 190
Asp Gly Ser Ala Leu Arg Val Tyr Lys Asp Glu Gly Asp Ile Ile Ala
195 200 205
Arg Thr Asn Pro Met Val Val Thr Ala Thr Thr Phe Leu Thr Phe Thr
210 215 220
Lys Ile Thr Thr Ile His Asp Val Val Ala Glu Glu Val Gly Ala Ile
225 230 235 240
Pro Ala Leu Ala Gly Thr Leu Ile Glu Ile Glu Thr Thr Val Asp Gly
245 250 255
Leu Asp Ser Val Tyr Asn Arg Tyr Gly Leu Thr Thr Gly Arg Asn Glu
260 265 270
Glu Thr Asp Thr Glu Leu Arg Gln Arg Tyr Leu Glu Ser Leu Ser Val
275 280 285
Thr Gly Val Gly Thr Leu Asp Ala Ile Val Ala Ala Val Lys Arg Val
290 295 300
Gln Gly Val Ser Asp Ala Ser Gly Val Glu Asn Asp Thr Glu Glu Thr
305 310 315 320
Ser Ser Glu Gly Leu Pro Pro Lys Ser Phe Lys Ile Val Val Val Gly
325 330 335
Gly Gln Asn Asp Asn Val Ala Gln Ala Ile Trp Asp Thr Lys Pro Ala
340 345 350
Gly Ile Arg Ala Tyr Gly Ala Ile Phe Gly Thr Ala Tyr Asp Leu Gly
355 360 365
Gly Leu Ser His Asn Ile Tyr Phe Ser Arg Pro Thr Pro Lys Tyr Ala
370 375 380
Phe Val Lys Val Ser Tyr Ser Leu Tyr Asn Glu Glu Ser Leu Thr Ile
385 390 395 400
Pro Glu Glu Asp Ile Arg Asp Ser Ile Ile Gln Gly Ile Asn Ala Tyr
405 410 415
Gly Arg Thr Leu Lys Val Gly Asn Asp Val Ile Pro Asn Arg Ile Tyr
420 425 430
Gly Tyr Ile Tyr Asn Val Ile Lys Gly Ile Glu Ile Asn Glu Ile Lys
435 440 445
Val Ala Leu Ser Asn Ser Gln Ser Val Pro Pro Ser Asp Gly Gln Tyr
450 455 460
Thr Thr Ala Arg Ile Thr Val Asp Gly Asp Gln Tyr Thr Val Trp Glu
465 470 475 480
Ser Ser Lys Tyr Thr Ile Thr Lys Glu
485
<210> 66
<211> 75
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 66
Leu Thr Met Leu Pro Ile Ser Ile Leu Ser Ile Leu Thr Ala Ser Asp
1 5 10 15
Leu Leu Trp Asp Thr Gly Gly Leu Ser Pro Cys Tyr Val Val Ala Leu
20 25 30
Ile Val Thr Ser Pro Ser Trp Tyr Val Thr His Ser Leu Leu Leu Tyr
35 40 45
Val Met Val Leu Ser His Tyr His Lys Leu Phe Asn Ile Ile Asn Ile
50 55 60
Val Leu Ser Lys Ile Phe Pro Ser Asn Cys Leu
65 70 75
<210> 67
<211> 396
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 67
Ile Cys Arg Ser Leu Ile Met Ala Val Gly Glu Ile Gln Ile Ser Ala
1 5 10 15
Leu Pro Gln Ala Ser Leu Pro Ile Asp Leu Ser Asp Ile Phe His Leu
20 25 30
Lys Gln Gly Ile Glu Asp Lys Arg Cys Thr Leu Glu Gln Leu Leu Ala
35 40 45
Pro His Ala Ser Leu Arg Asn Asn Pro His Gly Val Thr Lys Thr Gln
50 55 60
Ile Gly Leu Asp Asn Val Ile Asn Ala Leu Gln Leu Val Ala Ala Asn
65 70 75 80
Asn Leu Ser Asp Ile Val Asn Val Ala Glu Ala Arg Ala Asn Leu Gln
85 90 95
Ile Met Ser Ser Glu Glu Val Asn Asn Leu Val Gln Gln His Ile Lys
100 105 110
Asp Lys Ser Asn Pro His Asn Thr Thr Lys Ala Gln Val Gly Leu Ser
115 120 125
Asn Val Gln Asn Trp Thr Thr Ser Asn Leu Tyr Asn Glu Asp Ala Asp
130 135 140
Lys Tyr Ala Thr Ala Arg Ala Val Asn Asn Leu Tyr Lys Ala Val Gln
145 150 155 160
Ala Ser Tyr Pro Val Gly Thr Ile His Leu Ser Met Asn Ser Ala Asn
165 170 175
Pro Ser Thr Tyr Leu Ile Cys Gly Gly Thr Trp Glu Leu Val Ser Lys
180 185 190
Gly Arg Ala Leu Val Gly Tyr Asp Ser Asp Thr Arg Pro Ala Gly Ser
195 200 205
Val Phe Gly Ala Ser Thr Val Thr Leu Ser Val Asn Asn Met Pro Gln
210 215 220
His Ser His Ser Val Ser Leu Ser Gly Gly Gly His Thr His Asn Ala
225 230 235 240
Thr Val Ser Ile Ser Ala Phe Asp Tyr Gly Thr Lys Thr Thr Asn Ser
245 250 255
Phe Asp Tyr Gly Thr Lys Ala Thr Asn Thr Thr Gly Asn His Thr His
260 265 270
Ser Val Ser Gly Asn Thr Ser Thr Asn Gly Thr His Asn His Asp Val
275 280 285
Tyr Pro Arg Glu Asn Asp Gly Thr Gly Asn Ala Val Ala Asp Ser Asp
290 295 300
Gly Gly Gly Ala Arg Arg Thr Ser Arg Thr Ser Ser Asp Gly Ala His
305 310 315 320
Ser His Ser Phe Asn Val Thr Ser Gly Ala Ser Gly Asp His Ser His
325 330 335
Thr Val Gly Ile Gly Ala His Ser His Thr Val Gly Ile Gly Ser His
340 345 350
Thr His Ser Gly Thr Val Thr Val Ser Ser Ser Glu His Thr His Ser
355 360 365
Gly Asn Thr Asn Ser Val Gly Gly Gly Gln Ala Val Asn Ile Glu Gln
370 375 380
Pro Ser Phe Val Leu Tyr Val Trp Gln Arg Thr Ala
385 390 395
<210> 68
<211> 390
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 68
Met Ala Val Gly Glu Ile Gln Ile Ser Ala Leu Pro Gln Ala Ser Leu
1 5 10 15
Pro Ile Asp Leu Ser Asp Ile Phe His Leu Lys Gln Gly Ile Glu Asp
20 25 30
Lys Arg Cys Thr Leu Glu Gln Leu Leu Ala Pro His Ala Ser Leu Arg
35 40 45
Asn Asn Pro His Gly Val Thr Lys Thr Gln Ile Gly Leu Asp Asn Val
50 55 60
Ile Asn Ala Leu Gln Leu Val Ala Ala Asn Asn Leu Ser Asp Ile Val
65 70 75 80
Asn Val Ala Glu Ala Arg Ala Asn Leu Gln Ile Met Ser Ser Glu Glu
85 90 95
Val Asn Asn Leu Val Gln Gln His Ile Lys Asp Lys Ser Asn Pro His
100 105 110
Asn Thr Thr Lys Ala Gln Val Gly Leu Ser Asn Val Gln Asn Trp Thr
115 120 125
Thr Ser Asn Leu Tyr Asn Glu Asp Ala Asp Lys Tyr Ala Thr Ala Arg
130 135 140
Ala Val Asn Asn Leu Tyr Lys Ala Val Gln Ala Ser Tyr Pro Val Gly
145 150 155 160
Thr Ile His Leu Ser Met Asn Ser Ala Asn Pro Ser Thr Tyr Leu Ile
165 170 175
Cys Gly Gly Thr Trp Glu Leu Val Ser Lys Gly Arg Ala Leu Val Gly
180 185 190
Tyr Asp Ser Asp Thr Arg Pro Ala Gly Ser Val Phe Gly Ala Ser Thr
195 200 205
Val Thr Leu Ser Val Asn Asn Met Pro Gln His Ser His Ser Val Ser
210 215 220
Leu Ser Gly Gly Gly His Thr His Asn Ala Thr Val Ser Ile Ser Ala
225 230 235 240
Phe Asp Tyr Gly Thr Lys Thr Thr Asn Ser Phe Asp Tyr Gly Thr Lys
245 250 255
Ala Thr Asn Thr Thr Gly Asn His Thr His Ser Val Ser Gly Asn Thr
260 265 270
Ser Thr Asn Gly Thr His Asn His Asp Val Tyr Pro Arg Glu Asn Asp
275 280 285
Gly Thr Gly Asn Ala Val Ala Asp Ser Asp Gly Gly Gly Ala Arg Arg
290 295 300
Thr Ser Arg Thr Ser Ser Asp Gly Ala His Ser His Ser Phe Asn Val
305 310 315 320
Thr Ser Gly Ala Ser Gly Asp His Ser His Thr Val Gly Ile Gly Ala
325 330 335
His Ser His Thr Val Gly Ile Gly Ser His Thr His Ser Gly Thr Val
340 345 350
Thr Val Ser Ser Ser Glu His Thr His Ser Gly Asn Thr Asn Ser Val
355 360 365
Gly Gly Gly Gln Ala Val Asn Ile Glu Gln Pro Ser Phe Val Leu Tyr
370 375 380
Val Trp Gln Arg Thr Ala
385 390
<210> 69
<211> 690
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 69
Pro Leu Leu Arg Gly Lys Phe Ile Met Ala Asp Tyr Lys Leu Ser Glu
1 5 10 15
Leu Asn Ser Ile Asp Thr Ile Arg Ser Asp Asp Leu Leu His Val Arg
20 25 30
Val Lys Lys Arg Pro Lys Met Leu Gly Asp Glu Asp Arg Arg Met Thr
35 40 45
Tyr Gln Asp Phe Leu Ala Ser Phe Lys Leu Glu Arg Phe Val Gln Ile
50 55 60
Ala Gly Ser Thr Met Thr Gly Asp Leu Gly Ile Val Lys Leu Leu Tyr
65 70 75 80
Gly Gly Lys Ala Val Phe Asp Pro Thr Gly Ser Ser Glu Ile Thr Val
85 90 95
Gly Asp Val Leu Lys Thr Phe Lys Ile Asn Ala Asn Gly Leu Lys Leu
100 105 110
Thr Ile Ala Asp Ala Ser Arg Ser Ala Thr Val Tyr His Thr Leu Asn
115 120 125
Lys Pro Ser Pro Asn Glu Leu Gly Met Arg Thr Asn Glu Glu Asn Asp
130 135 140
Ala Arg Tyr Ala Arg Leu Ala Val Asn Asn Thr Phe Arg Gly Thr Gln
145 150 155 160
Ala Ile Leu Ser Asp Asn Glu Ala Leu Ile Ile Lys Asn Ile Thr Gln
165 170 175
Gly Ala Pro Leu Tyr Leu Arg Gly Gln Asp Ala Asp Gly Thr Asn Arg
180 185 190
Trp Tyr Leu Gly Asn Asp Asn Arg Gly Thr Asn Asn Leu Val Leu Lys
195 200 205
Asn Val Lys Ser Asp Val Ser Ile Ala Ile Leu Glu Asn Leu Val Thr
210 215 220
Val Asn Lys Thr Val Arg Ile Thr Gly Gln Val Gln Pro Ser Asp Trp
225 230 235 240
Thr Asn Ile Asp Ser Arg Tyr Ile Pro Ala Ala Thr Leu Ser Asn Leu
245 250 255
Ala Lys Leu Ser Asp Val Asn Thr Phe Arg Ser Ala Gln Ile Ile Asn
260 265 270
Gln Asn Gly Leu Leu Leu Gln Leu Lys Asn Thr Thr Gln Asp Ser Gln
275 280 285
Leu Tyr Phe Ala Gly Phe Asn Ala Asp Asn Val Arg Arg Trp Tyr Ile
290 295 300
Gly Asn Gly Glu Gln Lys Pro Glu Ser Phe Val Ile His Asn Asp Arg
305 310 315 320
Thr Ala Thr Thr Ile Phe Met Val Asn Asp Phe Leu Leu Asn Lys Thr
325 330 335
Val Arg Ile Thr Gly Gln Val Gln Pro Ser Asp Phe Ser Asn Leu Asp
340 345 350
Ala Arg Tyr Tyr Thr Gln Ser Ala Ala Asn Ala Arg Tyr Met Leu Ala
355 360 365
Ser Ile Thr Gly Ser Ala Ser Glu Ala Asp Gly Gly Asp Gly Val Ala
370 375 380
Trp Asp Ala Lys Thr Gly Leu Tyr Lys Val Thr Asn Thr Thr Gly Gln
385 390 395 400
Ser Ser Ser Leu Val Tyr His Met Phe Leu Gly Thr Gly Ser Thr Pro
405 410 415
Ser Ala Gln Leu Lys Phe Ser Phe Lys Asn Gly Gly Met Trp Tyr Arg
420 425 430
Thr Ser Arg Asp Ala Tyr Gly Phe Glu Val Asn Trp Ser Lys Val Tyr
435 440 445
Thr Glu Ala Gln Lys Pro Thr Pro Ser Glu Ile Gly Ala Tyr Thr Lys
450 455 460
Ala Glu Thr Asp Gln Lys Ile Ala Thr Ala Ile Ser Asp Ser Thr Asp
465 470 475 480
Leu Asn Lys Ile Tyr Pro Val Gly Ile Val Thr Trp Phe Asn Ser Asn
485 490 495
Val Asp Pro Asn Thr Ala Leu Pro Gly Leu Thr Trp Thr Tyr Leu Asn
500 505 510
Asn Gly Val Gly Arg Thr Ile Arg Ile Ala Ala Ala Asn Gly Ser Asp
515 520 525
Val Ala Thr Thr Gly Gly Ser Asp Ser Val Thr Leu Ser Val Gly Asn
530 535 540
Leu Pro Ser His Thr His Ser Phe Ser Ala Thr Thr Ser Ser Phe Asp
545 550 555 560
Tyr Gly Thr Lys Gly Thr Asn Ser Thr Gly Gly His Ser His Ser Val
565 570 575
Ser Gly Thr Thr Ser Ala Ala Gly Asn His Ala His His Leu Gly Leu
580 585 590
Leu Leu Val Asn Gly Gly Asp Ala Gln Tyr Gly Tyr Thr Thr Val Ser
595 600 605
Asn Ser Lys Thr Arg Thr Leu Asp Trp Leu Asp Arg Arg Asp Asn Lys
610 615 620
Phe Pro Asn Thr Asn Thr Thr Gly Asn His Thr His Thr Trp Ser Gly
625 630 635 640
Thr Thr Ser Gly Ala Gly Asp His Ser His Ser Val Gly Ile Gly Ala
645 650 655
His Ser His Thr Val Ser Gly Asn Thr Gly Ser Thr Gly Ser Gly Ser
660 665 670
Ala Phe Ser Val Thr Asn Gln Phe Tyr Lys Leu Met Ala Trp Val Arg
675 680 685
Thr Ala
690
<210> 70
<211> 682
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 70
Met Ala Asp Tyr Lys Leu Ser Glu Leu Asn Ser Ile Asp Thr Ile Arg
1 5 10 15
Ser Asp Asp Leu Leu His Val Arg Val Lys Lys Arg Pro Lys Met Leu
20 25 30
Gly Asp Glu Asp Arg Arg Met Thr Tyr Gln Asp Phe Leu Ala Ser Phe
35 40 45
Lys Leu Glu Arg Phe Val Gln Ile Ala Gly Ser Thr Met Thr Gly Asp
50 55 60
Leu Gly Ile Val Lys Leu Leu Tyr Gly Gly Lys Ala Val Phe Asp Pro
65 70 75 80
Thr Gly Ser Ser Glu Ile Thr Val Gly Asp Val Leu Lys Thr Phe Lys
85 90 95
Ile Asn Ala Asn Gly Leu Lys Leu Thr Ile Ala Asp Ala Ser Arg Ser
100 105 110
Ala Thr Val Tyr His Thr Leu Asn Lys Pro Ser Pro Asn Glu Leu Gly
115 120 125
Met Arg Thr Asn Glu Glu Asn Asp Ala Arg Tyr Ala Arg Leu Ala Val
130 135 140
Asn Asn Thr Phe Arg Gly Thr Gln Ala Ile Leu Ser Asp Asn Glu Ala
145 150 155 160
Leu Ile Ile Lys Asn Ile Thr Gln Gly Ala Pro Leu Tyr Leu Arg Gly
165 170 175
Gln Asp Ala Asp Gly Thr Asn Arg Trp Tyr Leu Gly Asn Asp Asn Arg
180 185 190
Gly Thr Asn Asn Leu Val Leu Lys Asn Val Lys Ser Asp Val Ser Ile
195 200 205
Ala Ile Leu Glu Asn Leu Val Thr Val Asn Lys Thr Val Arg Ile Thr
210 215 220
Gly Gln Val Gln Pro Ser Asp Trp Thr Asn Ile Asp Ser Arg Tyr Ile
225 230 235 240
Pro Ala Ala Thr Leu Ser Asn Leu Ala Lys Leu Ser Asp Val Asn Thr
245 250 255
Phe Arg Ser Ala Gln Ile Ile Asn Gln Asn Gly Leu Leu Leu Gln Leu
260 265 270
Lys Asn Thr Thr Gln Asp Ser Gln Leu Tyr Phe Ala Gly Phe Asn Ala
275 280 285
Asp Asn Val Arg Arg Trp Tyr Ile Gly Asn Gly Glu Gln Lys Pro Glu
290 295 300
Ser Phe Val Ile His Asn Asp Arg Thr Ala Thr Thr Ile Phe Met Val
305 310 315 320
Asn Asp Phe Leu Leu Asn Lys Thr Val Arg Ile Thr Gly Gln Val Gln
325 330 335
Pro Ser Asp Phe Ser Asn Leu Asp Ala Arg Tyr Tyr Thr Gln Ser Ala
340 345 350
Ala Asn Ala Arg Tyr Met Leu Ala Ser Ile Thr Gly Ser Ala Ser Glu
355 360 365
Ala Asp Gly Gly Asp Gly Val Ala Trp Asp Ala Lys Thr Gly Leu Tyr
370 375 380
Lys Val Thr Asn Thr Thr Gly Gln Ser Ser Ser Leu Val Tyr His Met
385 390 395 400
Phe Leu Gly Thr Gly Ser Thr Pro Ser Ala Gln Leu Lys Phe Ser Phe
405 410 415
Lys Asn Gly Gly Met Trp Tyr Arg Thr Ser Arg Asp Ala Tyr Gly Phe
420 425 430
Glu Val Asn Trp Ser Lys Val Tyr Thr Glu Ala Gln Lys Pro Thr Pro
435 440 445
Ser Glu Ile Gly Ala Tyr Thr Lys Ala Glu Thr Asp Gln Lys Ile Ala
450 455 460
Thr Ala Ile Ser Asp Ser Thr Asp Leu Asn Lys Ile Tyr Pro Val Gly
465 470 475 480
Ile Val Thr Trp Phe Asn Ser Asn Val Asp Pro Asn Thr Ala Leu Pro
485 490 495
Gly Leu Thr Trp Thr Tyr Leu Asn Asn Gly Val Gly Arg Thr Ile Arg
500 505 510
Ile Ala Ala Ala Asn Gly Ser Asp Val Ala Thr Thr Gly Gly Ser Asp
515 520 525
Ser Val Thr Leu Ser Val Gly Asn Leu Pro Ser His Thr His Ser Phe
530 535 540
Ser Ala Thr Thr Ser Ser Phe Asp Tyr Gly Thr Lys Gly Thr Asn Ser
545 550 555 560
Thr Gly Gly His Ser His Ser Val Ser Gly Thr Thr Ser Ala Ala Gly
565 570 575
Asn His Ala His His Leu Gly Leu Leu Leu Val Asn Gly Gly Asp Ala
580 585 590
Gln Tyr Gly Tyr Thr Thr Val Ser Asn Ser Lys Thr Arg Thr Leu Asp
595 600 605
Trp Leu Asp Arg Arg Asp Asn Lys Phe Pro Asn Thr Asn Thr Thr Gly
610 615 620
Asn His Thr His Thr Trp Ser Gly Thr Thr Ser Gly Ala Gly Asp His
625 630 635 640
Ser His Ser Val Gly Ile Gly Ala His Ser His Thr Val Ser Gly Asn
645 650 655
Thr Gly Ser Thr Gly Ser Gly Ser Ala Phe Ser Val Thr Asn Gln Phe
660 665 670
Tyr Lys Leu Met Ala Trp Val Arg Thr Ala
675 680
<210> 71
<211> 58
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 71
Met Leu Asp Thr Ile Pro Asn Leu Pro Gln Thr Gln Asp Ile Cys Leu
1 5 10 15
Gln Ala Ser Leu Val Arg Leu Leu Lys Gln Met Val Val Met Val Leu
20 25 30
His Gly Met Gln Lys Gln Val Phe Ile Arg Leu Leu Thr Pro Leu Asp
35 40 45
Ser Leu Leu His Leu Tyr Ile Thr Cys Phe
50 55
<210> 72
<211> 61
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 72
Met Val Gly Cys Gly Ile Glu His Gln Glu Met Pro Met Ala Ser Lys
1 5 10 15
Leu Ile Gly Leu Lys Phe Thr Arg Lys His Lys Asn Gln Pro Leu Gln
20 25 30
Arg Leu Val His Thr Leu Lys Leu Lys Leu Thr Arg Lys Leu Gln Gln
35 40 45
Gln Leu Val Thr Leu Gln Thr Leu Ile Lys Ser Ile Gln
50 55 60
<210> 73
<211> 12
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 73
Pro Asn Thr Ala Leu Pro Gly Leu Thr Trp Thr Tyr
1 5 10
<210> 74
<211> 85
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 74
Asn Ile Thr Thr Ile Ser Leu Ile Thr Val Cys Ser Asn Ala Val Ala
1 5 10 15
Leu Cys Ser Ser Lys Val Pro Lys Ile Ser Ser Ile Val Leu Glu Arg
20 25 30
Val Ser Val Cys Ser Thr Val Met Cys Val Arg Pro Arg Thr Ser Cys
35 40 45
Ala Thr Ser Thr Val Met Gly Val Arg Tyr Thr Asn Thr Ser Met Ala
50 55 60
Val Cys Asn Thr Thr Val Ser Thr Ala Cys Thr Arg Thr Arg Cys Lys
65 70 75 80
Thr Asn Ser Val Leu
85
<210> 75
<211> 63
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 75
Leu Tyr Thr Asn Leu Lys Ser Arg Cys Met Glu Leu Phe Ile Val His
1 5 10 15
Gln Phe Ile Asn Leu Leu His Met Val Leu Ile Asn Leu Ser Ile Ser
20 25 30
Lys Asp Thr Tyr Gln Val Pro Trp Ser Val Ser Ser His Leu Thr Lys
35 40 45
Asn Phe Gln Asn Lys Thr Ile Arg Arg Asn Ile Lys Trp Lys Pro
50 55 60
<210> 76
<211> 299
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 76
Lys Gly Val Ser Leu Pro Ala Glu Ile Lys Gly Ala His Gln Tyr Asn
1 5 10 15
Asn Ile Gly Gly Ile Phe Asp Ser Ala Thr Leu His Lys Leu Asp Ser
20 25 30
Leu Thr Asn Leu Gln Val Gly Ile Glu Phe Thr Pro Ala His Phe Glu
35 40 45
Glu Asn Asn Met Leu Glu Tyr Val Gln Lys Met His Asn Gln Tyr Ile
50 55 60
Gln Thr Asp Gly Phe Val Gly Ile Leu Tyr Arg Pro Thr Tyr Gly Thr
65 70 75 80
Met Lys Cys Leu Val Glu Leu Ile Phe Gly Tyr Ser Ala Ile Asn Phe
85 90 95
Pro Leu Gly Leu Phe Thr Asp Val Ser Arg Gln Tyr Trp Gln Leu Asp
100 105 110
Val Glu Gly Glu Asn Thr Ile Tyr Ile Gln Phe Leu Val His Cys Pro
115 120 125
Ala Leu Ala Met Ser Asn Ala Ala Ile Asp Ala Ala Asn Trp Ala Asn
130 135 140
Val Leu Met Tyr Arg Asp Thr Ile Asp Gly Lys Met Lys Thr Val Leu
145 150 155 160
Thr Glu Leu Gln Asp Val Val Cys Gly Asn His Gly Glu Phe Ser Arg
165 170 175
Trp Gln Val Gly Tyr Ile Arg Gly Gly Ala Asp Glu Leu Trp Asp Gln
180 185 190
Glu Ser Ser Tyr Asn Ser Ser Leu Trp Arg Lys Phe Asp Asp Ser Trp
195 200 205
Ile Thr Trp Arg Glu Gly Asp Asn Glu Gly Trp Thr Arg Tyr Arg Leu
210 215 220
Glu Gly Leu Lys Asn Glu Gly Asn Leu Phe Trp Leu Ala Glu Gly Ile
225 230 235 240
Val Gln Arg Thr Phe Val Gln Lys Ser Thr Leu Leu Pro Phe Gly Thr
245 250 255
Arg Met Asp Phe Arg Phe Gln Pro Pro Ala Phe Ala Ser Ile Val Asp
260 265 270
Gly Thr Arg Thr Thr Arg Leu Glu Gly Val Ser Leu Val His Asn Leu
275 280 285
Ile Asn Lys Tyr Ser Ser Asp Ala Gln Ser Met
290 295
<210> 77
<211> 173
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 77
Ile Ser Lys Val Ala Leu Pro Ser Ser Asp Gly Phe Asp Cys Thr Phe
1 5 10 15
Val Asn Thr Arg Leu Ser Tyr Ser Phe Ser Lys Lys Phe Ser Lys Gly
20 25 30
Lys Cys Thr Val Ala Ser Ser Val Thr Leu Trp Lys Thr Val Trp Asn
35 40 45
Thr Thr Pro Ala Tyr Gly Lys Ala Leu Thr Arg Thr Pro Ala Pro Pro
50 55 60
Ile Thr Lys Thr Ser Ser Ser Ile Glu Leu Ser Arg Tyr Phe Lys Asn
65 70 75 80
Ala Pro Asn Asp Ser Leu Leu Ala Leu Ala Tyr Ile Thr Ser Ser Leu
85 90 95
Pro Lys Ser Met Pro Lys Tyr Gly Thr Glu Phe Val Lys Ile Arg Leu
100 105 110
Ala Arg Ser Gly Arg Gly Leu Leu Pro Arg Val Leu Lys Val Ser Leu
115 120 125
Pro Met Thr Thr Lys Leu Phe Leu Val Ser Leu Leu Asn Ser Cys Met
130 135 140
Ser Cys Leu Cys Arg Gly His Gly Ile Pro Val Gly Val Pro Asn Ser
145 150 155 160
Pro Phe Ser Pro Leu Ala Lys Ile Asn Leu Ile Ile Leu
165 170
<210> 78
<211> 181
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 78
Phe His Gly Lys Val Gln Pro Val Tyr Ile Glu Phe Thr Ala Lys Gly
1 5 10 15
Asp Glu Ser Pro Lys Ser Gln Val Lys Thr Phe Val Arg Asn Asp Tyr
20 25 30
Leu Lys Glu Phe Phe Asn Glu Phe Pro Leu His Val Thr Ala Glu Glu
35 40 45
Thr Val Lys His Phe Val Thr His Phe Val Val Gly Ala Tyr Pro Leu
50 55 60
Ala Asn Val Leu Val Gly Ala Gly Gly Ile Val Phe Val Asp Glu Asp
65 70 75 80
Ile Ser Ser Asp Leu Tyr Lys Leu Phe Ala Gly Phe Ser Glu Lys Ser
85 90 95
Ala Lys Ala Tyr Met Val Asp Asp Lys Gly Phe Asp Ile Gly Leu Tyr
100 105 110
Pro Val Ser Asn Thr Leu Ile Leu Ser Ala Arg Glu Pro Leu Pro Lys
115 120 125
Ser Gly Leu Thr Lys Phe Thr Glu Asn Gly Met Val Val Leu Asn Asn
130 135 140
Lys Thr Leu Arg Lys Phe Glu Gln Met Asp Gln Lys His Arg Pro Trp
145 150 155 160
Pro Met Gly Thr Pro Thr Gly Phe Glu Gly Asn Glu Gly Ser Ala Phe
165 170 175
Ile Leu Lys Ile Met
180
<210> 79
<211> 71
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 79
Met Trp Thr Thr Asp Arg Cys Ser Ile Arg Leu Ile Ala Val Phe Asn
1 5 10 15
Phe His Leu Phe Asn Leu Lys Asn Trp Phe Lys Asn Gln Tyr Lys Val
20 25 30
Tyr Thr Val Ile Phe Phe Ile Gln Asn Thr Ile Lys Val Leu Arg Tyr
35 40 45
Lys Val Glu Glu Ala His Arg Ile Ser Val Arg Ser Asn Pro Asp Ser
50 55 60
Val Pro Tyr Ile Leu Tyr Ser
65 70
<210> 80
<211> 86
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 80
Lys Thr Phe Gly Lys Lys Ile Phe Trp Asn Ile Val Ser Lys Val Val
1 5 10 15
Lys Ser Arg His Tyr Ile Phe His Pro Ser Pro Gln Tyr Ser Gly Tyr
20 25 30
Ala Trp Phe Ala Ile Ile Glu Asn Cys Gly Thr Cys Arg Thr Ser Glu
35 40 45
Glu Ala Ile His Gly Asn Val Ile Glu Ser Val Glu Gly Gln Leu Asp
50 55 60
Thr Cys Gly Cys His Pro Cys Arg Ile Pro Thr Gly Asp Ser Glu Tyr
65 70 75 80
Cys His Glu Ile Lys Met
85
<210> 81
<211> 109
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 81
Asp Ala Leu Arg Leu Asn Phe Asn Val Gly Phe Lys Glu Cys Val Glu
1 5 10 15
Leu Met Tyr Glu Leu Ser Val Asn Gly Thr Leu His Ile Ile Asn Ser
20 25 30
Val Arg Ala Gln Ser Ile Gly Cys Ile Lys Ala Val Gln Glu Gln Ser
35 40 45
Leu Gly Arg Ser Lys Ile Glu Arg Lys Phe Arg His Ala Val Phe Asp
50 55 60
Arg Met Glu Asp Val Asp Met Pro Cys Ile Tyr Ser Glu Met Glu Ala
65 70 75 80
Glu Arg Asp Ala Glu Trp Asp Asp Gln Pro Ala Tyr Pro Asp Phe Val
85 90 95
Glu Asn Met Lys Arg Arg Gly Asn Ala His Leu Thr Leu
100 105
<210> 82
<211> 70
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 82
Glu Asp Lys Lys Lys Arg Lys Arg Ser Thr Asp Ile Leu Ser Asp Ile
1 5 10 15
Asn Thr Ala Arg Val Lys Glu Arg Glu Asp Asn Leu Tyr Asp Asp Leu
20 25 30
Ala Lys Glu Ile Gln Ser Asp Asp Gln Cys Ile Val Thr Phe Val Thr
35 40 45
Phe Gly Asp Thr Asp Asp Ile Ile Lys Gly Tyr Gly Ser Arg Pro His
50 55 60
Leu Lys Lys Leu Ala Met
65 70
<210> 83
<211> 124
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 83
Phe Tyr Arg Lys Thr Thr Lys Ala Trp Thr Pro Ile Thr Tyr Thr Thr
1 5 10 15
Lys Val Ala Ser Ile Phe Gln Asn His Gln Glu Phe Phe Phe Gly Asp
20 25 30
Leu Leu Phe Ile Glu His Gln Thr Lys Leu Tyr Arg Pro Val Thr Glu
35 40 45
Asp Trp Thr Val Ala His Asn Ser Cys Cys Ala Asn Leu Asn Arg Phe
50 55 60
Ser Ile Asp Lys Val Trp Ile Leu Ser Lys Phe Leu Trp Val Cys Trp
65 70 75 80
Ala Val Leu Asp Ser His Trp Glu Asp Tyr Ile Cys Gln His Asn Gly
85 90 95
Ser Val Asn His Leu Thr Lys Phe Val Glu Phe Cys Thr Arg Leu Asp
100 105 110
Thr Ser Leu Asn His Leu Thr Val His Asn Lys Thr
115 120
<210> 84
<211> 89
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 84
Leu Thr Arg Pro Asp Leu Thr Leu Pro Cys His Thr Val Pro Asn Arg
1 5 10 15
Thr Leu Pro Tyr Leu Thr Ser Leu Lys Asn Pro Leu Arg Arg Leu Leu
20 25 30
Ser Asp Ala Gly Glu Tyr Pro Leu Val Tyr Ala Ala Gln Tyr Thr His
35 40 45
Gln Ile Phe Tyr Val Asn Asn Phe Leu Tyr Lys Ser Pro Asp Ser Tyr
50 55 60
Met Cys Tyr Lys Thr Glu Asp Cys Glu Gln Asn Thr His Cys Lys Arg
65 70 75 80
Cys Val Gln Lys Leu Thr Cys Asn Val
85
<210> 85
<211> 384
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 85
Glu Tyr Asp Arg Ile Ala Leu Gly Val Pro Phe Gln Leu Ser Gly Lys
1 5 10 15
Arg Thr Met Lys Gln Phe Gln Val Thr Leu Pro Lys Gly Ile Phe Glu
20 25 30
Glu Pro His Glu Ala Leu Tyr Gln Lys Lys Glu His Thr Thr Lys Trp
35 40 45
Ser Val Asp Val Phe Val Asn Pro Leu Thr Glu Val Val Phe Lys Gly
50 55 60
His Gly Arg Lys Asp Thr Lys Val Gly Thr Val Lys Phe Glu Glu Thr
65 70 75 80
His Met Lys Lys His Lys Ile Leu Phe Tyr Ser Arg His Cys Cys Glu
85 90 95
Tyr Phe Ser Asp Glu Ser Ile Ser Arg Tyr Met Ala Gly Glu Phe Lys
100 105 110
Ala Glu Val Trp Lys Asp His Ala Glu Asp Phe Glu Glu Trp Ser Asn
115 120 125
Val Arg Arg Ser Lys Val Pro Val Val Lys Cys Gly Asp Arg Phe Asp
130 135 140
Asn Leu Pro Ser Thr Glu Ile Ile Asp Arg Arg Glu Pro Trp Thr Lys
145 150 155 160
Thr Ser Asp Cys Ile Asp Tyr Trp Tyr Phe Lys Leu Leu His Arg Asp
165 170 175
Pro Asn Asp Glu Asn Lys Ile Met Ser Val Ile Asp Glu Leu Asp Trp
180 185 190
Gly His Ala Tyr Ile Glu Gly Asp Phe Glu Asp Val Asp Lys His Leu
195 200 205
Arg Leu Met Ala Met Ile Asp Pro Ile Leu Glu Thr Pro Ile Lys Tyr
210 215 220
Val Thr Asn Glu Arg Ser Tyr Ala Thr Phe Val Ser Gly Ile Ser Leu
225 230 235 240
His Arg Ser Ile Arg Cys Arg Val Gly Asp Leu Lys Pro Gln Ala Asp
245 250 255
Ala Pro Phe Lys Leu Tyr Lys Ala His Ser Val Lys Ala Ala Asp His
260 265 270
Ala Leu Gln Val Gly Val Lys Glu Thr Asn Gln Ala Ser Asp Lys Thr
275 280 285
Tyr Ala Tyr Gly Lys Arg Asp Val Gln Lys Lys Tyr Lys Ala Ala Val
290 295 300
Glu Phe Leu Ala Gln Gln Glu Ala Asn Arg Glu Asn Lys Lys Gly Ile
305 310 315 320
Asn Lys Pro Glu Ala Thr Tyr Glu Glu Phe Met Met Lys Gly Asn Glu
325 330 335
Lys Gly Tyr Thr Thr Ile Val Lys Asp Gly Val Ala Val Cys Ser Trp
340 345 350
Val Asn Phe Ser Gly Asp Lys Asn Gln Lys Tyr Leu Ile Thr Met Lys
355 360 365
Leu Arg Gln Thr Ile Gly Leu Thr Ala Pro Met Val His Thr Phe Ser
370 375 380
<210> 86
<211> 90
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 86
Leu Thr Ile Phe Ala Ile Ser Ile Arg Cys Cys Cys Leu Arg Val Trp
1 5 10 15
Ala Ser Asp Asn Asn Asn Ile Pro Ala Tyr Ser Ile Pro Ile Glu Phe
20 25 30
Val Asp Phe Ala Val Leu His Leu Trp Arg Glu Ala Val Arg Gly Ile
35 40 45
Phe Val Phe Ile Arg Trp Val Thr Ala Ser Gln Lys Asn Tyr Ser Lys
50 55 60
Cys Asn His Cys Phe His Thr Thr Leu Asn Leu Ile Val His Asp Asn
65 70 75 80
Leu Pro Leu Cys Met Phe Leu Asn Ser Glu
85 90
<210> 87
<211> 101
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 87
Lys Arg Lys Gly Gly Ile Glu Val Asp Ile Val Gln Val Asp Gly Ala
1 5 10 15
Val Asp Phe Lys His Ile Ile Ser Glu Val Thr Tyr Thr Trp Gly Glu
20 25 30
Ile Arg Val Leu Glu Gly Glu Arg Pro Val Ile Pro Asn Arg Gln Ala
35 40 45
Val Thr Ala Thr Ser Tyr Val Val Asp Tyr Cys Tyr Ile Tyr His Val
50 55 60
Leu Val Gln Met Asn Thr Glu Asp Met Leu Leu Leu Leu Thr His Asn
65 70 75 80
Val Met Lys Ala Met Asp Ile Leu His Gln Gln Lys Leu Thr Gln Ala
85 90 95
Asp Ser Leu Leu Leu
100
<210> 88
<211> 188
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 88
Ser Leu Lys Ala Phe Glu Gly His Leu Lys Ile Ala Ala Glu Cys Tyr
1 5 10 15
Ala Lys His Ala Glu Glu Pro Thr Asp Phe Met Gly Leu His Tyr Asn
20 25 30
Lys Gly Gln Leu Arg Ile Met Ala Arg Phe Arg Lys Ser Ile Lys Gln
35 40 45
Val Gly Lys Pro Leu Gly Ser Pro Ser Glu Arg Ser Arg Asn Cys Ser
50 55 60
Asn Gln Ser Ala Thr Cys Val Arg Leu Asn Leu Tyr Arg Asn Asp Trp
65 70 75 80
Gly Asp Gly Asn Lys His Asp Val Gln Leu Thr Lys Asp Pro Leu Tyr
85 90 95
Gly Asp Met Tyr Leu Ile Val Leu Arg Asn Ala Gln Tyr Gly Arg Gly
100 105 110
Asp Ile Leu Leu Gln Val Ser Lys His Gly Asp Thr Asn Thr Arg Tyr
115 120 125
Asn Gly Ala Arg Lys Gly Gly Phe Arg Ser Asn Phe Asn Ala Asn Gly
130 135 140
Glu Arg Phe Lys Trp Thr Phe Val Gly Thr Glu Pro Asp Tyr Asp Leu
145 150 155 160
Leu Glu Lys Leu Arg Ile Glu Thr Ile Met Glu Val Gly Asp Ile Tyr
165 170 175
Lys Trp Gly Ala Arg Val Leu Arg Ala Gln Ala Leu
180 185
<210> 89
<211> 119
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 89
Met Glu Leu His Lys Leu Phe Ile Ser Phe Pro Thr Ile Asp Ser Leu
1 5 10 15
Leu Thr His Pro Glu Arg Cys Phe Phe Ser Ser His Val Gly Phe Ile
20 25 30
Ser Val Tyr Ala Thr Leu Leu Lys Leu Glu Ile Leu Ile Ile Lys Cys
35 40 45
Pro Phe Ser Phe Gly Val Asn Thr Asp Ile Phe Tyr Asn Thr Ser Glu
50 55 60
Asp Phe Cys Ile Pro His Thr Pro Met Phe Asn Leu Trp Phe Thr Asn
65 70 75 80
Thr Asn Thr Ser Thr Pro Cys Ser Gln Ala Met Ser Thr Phe Ile Thr
85 90 95
Leu Trp Ser Trp Ser Ile His Pro Leu Asn Thr Arg Leu Asp Lys Arg
100 105 110
Ile Leu Lys Glu Ile Thr Pro
115
<210> 90
<211> 910
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 90
His Val Glu Leu Trp Ser Lys Gly Val Lys Gly Thr Gly Thr Val Leu
1 5 10 15
Thr Glu Ser Lys Leu Gly Leu Asn Lys Ser Ala Gln Gly Tyr Met Trp
20 25 30
Asp Thr Ile Ala Lys Val Glu Gln Thr Met Ser Glu Pro Cys Glu Trp
35 40 45
Ser Asn Glu Asp His Tyr Ala Leu Leu Leu Arg Ala Gly Ile Ala Pro
50 55 60
Lys Arg Gly Asn Leu Lys Thr Leu Gly Glu Glu Cys Ala Arg Arg Cys
65 70 75 80
Ala Leu Asn Ile Ala Glu Asn Gln Ile Gln Ala Glu Ser Gly Met Leu
85 90 95
Leu Tyr Asn Leu Leu Lys His Pro Ser Lys Cys Trp Leu Trp Ala Gly
100 105 110
Ala Val Lys Val Phe Gly Gly Lys Ser Ile Ala Ala Thr Lys Gly Phe
115 120 125
Val Glu Glu Leu Ala Lys Lys Thr Ala Lys Tyr Asp Ala Glu Leu Asp
130 135 140
Asp Leu Leu Lys Lys Ile Lys Pro Leu Arg Ile Phe Tyr Thr Gln Lys
145 150 155 160
Thr Leu Ala Gly Glu Gln Thr Thr Ala Ala Lys Ile Thr Lys Ala Phe
165 170 175
Lys Glu Asp Gly Ala Gly Phe Leu Leu Ala Tyr Ile Gly Asn Lys Ala
180 185 190
Lys Lys Arg Gly Lys Ser Ile Glu His Leu Leu Asn Glu Asp Gln Thr
195 200 205
Ala Arg Cys Thr Leu Ile Asp Gln Glu Asp Asn Leu Asn Phe Tyr Ile
210 215 220
Ser Asn Leu Thr His Ala Asp Thr Gly Ser Tyr Val Val Tyr Leu Asp
225 230 235 240
Asn Glu Ser Asp Tyr Arg Leu Tyr Lys Asp Ile Glu Pro Asn Leu Tyr
245 250 255
Glu Asp Phe His Lys Cys Tyr Tyr Arg Gly Asp Glu Gln Arg Ile Phe
260 265 270
Glu Ile Glu Glu Lys Gly Gln Thr Val Ala Lys Thr Phe Asp Lys Asp
275 280 285
Gly Met Phe Asn Cys Leu Leu Val Leu Gln Ala Ser Asn Met Asp Val
290 295 300
Ser Val Val Asn Thr Gly Glu Glu Cys Ile Trp Val Ala Arg Met Pro
305 310 315 320
Glu Gly Tyr Val Ala Ala Gly Ser Pro Val Asn Val Ile Gly Tyr Gln
325 330 335
Thr Met Arg Gly Thr Ser Thr Gly Phe Val Met Ala Gly Ala Ser Leu
340 345 350
Arg Gly Asp Pro Arg Ile Gln Asn Leu Trp Gly Lys Glu Asp Asp Lys
355 360 365
Ser Asn Glu Ile Thr Arg Arg Arg His Met Leu Thr Tyr Tyr Lys Ala
370 375 380
Ile Lys Gln Gly Leu Glu Gly Glu Ile Thr Asp Tyr Ser Ser Glu Thr
385 390 395 400
Leu Lys Pro Ser Thr Arg Ile Leu Cys Pro Glu Leu Leu Asp Thr Tyr
405 410 415
Lys Lys Val Ser Trp Asn His Glu Ile Tyr Gln Val Gly Glu His Glu
420 425 430
Val Tyr Ser Leu Gly Cys Arg Glu Val Met Glu Gln Trp Lys Pro His
435 440 445
Lys Thr Ile Met Lys Lys Asn Asp Lys Phe Arg Cys Val Lys Val Pro
450 455 460
Arg Gly Asp Ser Asp Lys Lys Tyr Asn Trp Asp Asp Pro Ile Trp Gly
465 470 475 480
Val Ser Lys Leu Tyr Asp Lys Val Val Ala Val Gln Thr Met Arg Ser
485 490 495
Val Glu Phe Glu Ile Pro Thr Tyr Pro Ala Asp Ile Leu Gly Thr Tyr
500 505 510
Arg Ser Ser Glu Leu Glu Phe His Asn Val Val His Ser Asn Tyr Tyr
515 520 525
Lys Val Thr Lys Val Thr Lys Val Pro Lys Trp Asp Lys Cys Lys Lys
530 535 540
Gly Ile Lys Ala Asn Leu Glu Asn Cys Ile Ala Arg Ala Asp Lys Met
545 550 555 560
Ala Val Phe Gly Glu Lys Tyr Glu Glu Gly Thr Val Ser Asn Thr Pro
565 570 575
Thr Tyr Cys Asn Arg Lys Glu Ile Asn Phe Ile Lys Thr Thr Pro Lys
580 585 590
Gly Phe Pro Lys Ile Glu Tyr Thr Gln Met Glu Gly Asn Arg Val Gln
595 600 605
Gln Arg Tyr Lys Thr Lys Pro Tyr Lys Thr Ile Arg Lys Leu Gly Asp
610 615 620
Ala His Gly Phe Thr Glu Val Tyr Glu Asn Trp Ala Lys Ala Phe Glu
625 630 635 640
Glu Gly Thr Val Lys Gly Lys Thr Lys Ile Thr Pro Pro Leu His Pro
645 650 655
Glu Val Glu Ser Ala Leu Glu Asn Thr Leu Lys Asp Leu Glu Lys Val
660 665 670
His Phe Glu Met Leu Glu Lys Asp Ala Lys Trp Gly Asn Ile Ala Gln
675 680 685
Gln Ser Met Trp Phe Ser Thr Glu Lys Ala Arg Met Tyr Thr Glu Tyr
690 695 700
Thr Asp Ile Gly Cys Lys Lys Leu Lys Leu Tyr Glu Asn Asp Leu Ala
705 710 715 720
Arg Lys Thr Lys Ala Asn Ile Arg Ile Asp Glu Val Val Arg Thr Phe
725 730 735
Ile Glu Ala Asn Trp Val Gly Trp His Glu Ile Glu Pro Lys Arg Val
740 745 750
Gly Val Arg Ala Gly Trp Ala Ala Leu Gly His Ala Gly Lys Tyr Gly
755 760 765
Lys Pro Thr Pro Arg Asp Met Trp Gln Val Arg Ser Gln Ile Leu Ser
770 775 780
Asp Gln Phe Phe Asn Ser Trp Pro Lys Cys Lys Pro Asn Asp Phe Asn
785 790 795 800
Trp Leu Asp Pro Ala Ile His Asn Phe Val Trp Trp Asp Tyr Gly Leu
805 810 815
Gly Asn His Ile Cys Leu Arg Lys Ala Lys Leu Ala Phe Glu Ile Gly
820 825 830
Glu Arg Leu Gly Gly Lys Ile Tyr Lys Thr Tyr Lys His Gly Leu Ile
835 840 845
Pro Glu Asp Thr Phe Val Trp Val Pro Pro Glu Glu Asp Asp Ser Lys
850 855 860
Tyr Asp Ala Phe Ala Met Val His Val Lys Thr Phe Glu Leu Leu Leu
865 870 875 880
Gly Asp Thr Glu Thr Asp Gly Val Leu His Leu Asn Lys Phe Asp Val
885 890 895
Asn Glu Met Lys Val Gly Glu Leu Ile Met Leu Arg Met Cys
900 905 910
<210> 91
<211> 154
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 91
Asp Lys Arg Leu Lys Leu Asn Ser Tyr Leu Lys Glu Ala Tyr Met Gln
1 5 10 15
Lys Met Phe Val His Pro Asp Ile Ala Asp Phe Val Arg Ala Leu Gln
20 25 30
Lys Leu Glu Glu Leu Asp Ile Ala Ala Gln Arg Glu Tyr Ala His His
35 40 45
His Arg Arg Met Val Asp Ile Gln His Glu Ile Glu Leu Ser Glu Asn
50 55 60
Tyr Glu Asp Asp Leu Lys Cys Ser Val Phe Glu His Met Lys Asp Val
65 70 75 80
Ala Thr Ala Arg Arg Lys Ala Lys Asp Thr Val Ala Leu Leu Asp Ser
85 90 95
Leu Lys Lys Arg Leu Gln Ser Gly Thr Asp Leu Cys Asn Leu Ala Ser
100 105 110
Leu Ile Asn Asp Val Glu Thr Ser Trp Asp Arg His Tyr Tyr Pro Arg
115 120 125
Ser Glu Lys Thr Leu Asp Phe Ser Ser Ser Glu Asn Leu Lys Cys Ser
130 135 140
Arg Lys Lys Leu Asn Gln Leu Arg Glu Lys
145 150
<210> 92
<211> 270
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 92
Glu Arg Asn Asn Met Asn Ile Asp Val Glu Lys Met Leu Glu Glu Lys
1 5 10 15
Gly Ile Asp Glu Ala Phe Leu Ile Asn Ala Val Glu Ser Met Ala Met
20 25 30
Ile Leu Arg Gly Asn Asn Tyr Thr Glu Gln Phe Met Pro Ala Ser Tyr
35 40 45
Asn Leu Pro Glu Asp Lys Ser Asp Asn Gly Ala Phe Ala Glu Gln Val
50 55 60
Asn Met Met Leu Cys Asp Lys Thr Leu Ala Leu Ala Ser Ile Val Val
65 70 75 80
Gly Val Asp Ala Leu Ala Glu Phe Ile Phe Tyr Glu Met Gln Lys Asn
85 90 95
Asp Tyr Ile Ala Met Thr Thr Glu Glu Lys Asp Leu Leu Leu Leu Ala
100 105 110
Gly Asn Asp Glu Leu Ile Asp Leu Leu Ile Ser Ser Thr Ser Ala Val
115 120 125
Met Ala Ala Leu Glu Lys Arg Met Glu Glu Lys Leu Ser Arg Tyr Glu
130 135 140
Lys Leu Asp Trp Gly Val Glu Glu Leu Asn Pro Arg Ile Lys Glu Ile
145 150 155 160
Ile Gln Gln Thr Lys Glu Ser Leu Glu Glu Ala Val Glu Asn Asp Ser
165 170 175
Val Ala Asp Ile Glu Pro Leu Gln Val Lys Val Ala Leu Met Ile Pro
180 185 190
Ala Ile Ser Thr Met Met Asn Ile Val Ala Leu Ile Gln Leu Ser Gln
195 200 205
Met Met Gly Val Ser Ile Glu Phe Ile Glu Glu Met Thr Gly Gly Val
210 215 220
Ser Met Gln Ala Ile Asn Asp Val Leu Val Asn Ile Gly Ala Ser Ile
225 230 235 240
Ile Glu Glu Lys Leu Arg Ile Asn Phe Gly Asp Asp Phe Val Lys Gln
245 250 255
Ile Ser Ala Met Ala Glu Lys Glu Tyr Ala Glu Gln Leu His
260 265 270
<210> 93
<211> 247
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 93
Met Ser Asn Ile Ile Ala Phe Thr Gly Lys Ala Arg Ser Gly Lys Asp
1 5 10 15
Thr Ser Cys Ser Ile Val Lys Asn Ile Leu Glu Asp Glu His Gly Tyr
20 25 30
Asn Val Ala Val Met Ala Phe Ala Asp Asn Leu Lys Leu Ser Ala Ser
35 40 45
Lys Ile Phe Asp Leu Thr Trp Asn Asp Leu Tyr Gly Glu Thr Lys Glu
50 55 60
Thr Pro Gln Val Phe Asp Leu Ser Tyr Pro Glu Leu Met Phe Lys Val
65 70 75 80
Thr Glu Ala Met Glu Phe Thr Phe Arg Asp Glu Arg Tyr His Met Asp
85 90 95
Phe Lys Leu Met Ser Glu Leu Thr Gly Arg Leu Ile Met Glu Leu Lys
100 105 110
Lys Val Ala Lys Pro Thr Leu Leu Thr Arg Leu Gly Phe Ser Lys Lys
115 120 125
Tyr Lys Phe Ser Ser Arg Gln Ile Gln Gln Ile Trp Gly Thr Glu Val
130 135 140
Val Arg Lys Val Met Gly Asp Lys Phe Trp Ser Lys Asp Leu Glu Lys
145 150 155 160
Arg Met Val Arg Phe Tyr Glu Ser Cys Ser Leu Arg Asn Gln Tyr Gly
165 170 175
Phe Val Leu Ile Ser Asp Leu Arg Phe Asp Ser Glu Ala Glu Trp Leu
180 185 190
Ser Arg Phe Ala His Gln Thr Ile Glu Val Lys Arg Asp Asn Val Asp
195 200 205
Lys Ile Ser Ser His Val Ser Glu Asn Gly Leu Ser Thr Lys Tyr Glu
210 215 220
Arg Asp Ile Ile His Asn Asn Gly Thr Leu Thr Asp Leu Glu Asn Lys
225 230 235 240
Leu Arg Ala Ile Ile Lys Val
245
<210> 94
<211> 670
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 94
Arg Gln Asn Tyr Asn Glu Glu Val Leu Met Ser Lys Gly Arg Lys Leu
1 5 10 15
Lys Glu Ala Gly Gln Phe Val Gly His Cys Ala Cys Pro Arg Cys Gly
20 25 30
Ser Ser Asp Ala Gly Ser Ile Tyr His His Asp Asp Asp Ser Tyr Ser
35 40 45
Met Thr Cys Phe Ser Cys Asn Lys Gly Phe Pro Glu Trp Asp Phe Asp
50 55 60
Lys Gly Gln Ile Val Ser Thr Tyr Ser Thr Gly Ser Asp Asn Lys Asn
65 70 75 80
Arg Thr Phe Arg Gly Met Asp Leu Asp Asp Val Lys Asp Asn Leu Glu
85 90 95
Ala Met Asp Leu Lys Asp Arg Lys Ile Pro Ala Lys Val Leu Glu Arg
100 105 110
Leu Gly Ile Lys Val Asp Ile Asp Ser Asp Gly Glu Ile Asp Ala His
115 120 125
Phe Tyr Pro Thr Tyr Lys Arg Asn Glu Asp Gly Lys Leu Glu His Val
130 135 140
Gly Tyr Arg Val Arg His Arg Tyr Pro Glu Asp His Pro Lys Glu His
145 150 155 160
Leu Arg Gly Lys Leu Lys Asp Phe Ser Gly Gly Val Gly Asp Ile Lys
165 170 175
Gly Glu Leu Ala Met Phe Gly Ser Trp Ile Ala Pro Glu Gly Gly Asn
180 185 190
Arg Leu Phe Ile Trp Glu Gly Glu Met Glu Cys Ala Thr Ala Ile Tyr
195 200 205
Met Thr Ser Leu Ala Ile Lys Asp Lys Ser Arg Arg Lys Asn Tyr Cys
210 215 220
His Val Ser Val Pro Ser Gly Ala Asn Ile Lys Ser Ile Lys Asp Asn
225 230 235 240
Tyr Gln Tyr Ile Thr Ser Phe Asp Glu Ile Tyr Leu Cys Phe Asp Asn
245 250 255
Asp Glu Ala Gly Ala Lys Ala Thr Lys Glu Ala Ala Gly Ile Leu Pro
260 265 270
Ile Glu Lys Val Arg Leu Phe Gln Tyr Pro Glu Gly Val Lys Asp Leu
275 280 285
Asn Glu Trp Trp Thr Lys Phe Tyr Lys Glu Lys Asp Thr Val Leu Glu
290 295 300
Gly Phe Lys Gln Arg Ile Tyr Asn Ala Pro Arg Tyr Cys Pro Ala Gly
305 310 315 320
Ile Lys Asn Phe Ala Asp Gly Phe Glu Ala Met Lys Asn Arg Gly Gln
325 330 335
Ile Pro Leu Ile Pro Phe Pro Glu Ser Phe Gly Asp Leu Asn Arg Leu
340 345 350
Thr Tyr Gly Gly Tyr Gly Leu Gly Glu Ile Thr Thr Ile Ala Ala Pro
355 360 365
Ser Ser Val Gly Lys Ser Ala Tyr Thr Arg Glu Met Ile Tyr Ser Ala
370 375 380
Trp Lys Glu Thr Asp Tyr Asn Ile Gly Val Ile Pro Val Glu Asp Thr
385 390 395 400
Tyr Glu Glu Leu Met Glu Met Leu Cys Ala Ile His Leu Ser Lys Gln
405 410 415
Ile Ser Glu Ile Pro Tyr Asp Glu Arg Asp Trp Asp Glu Leu Lys Gly
420 425 430
Ala His Ala Glu Leu Ser Lys Gly Arg Arg Ile His Ile Val Asp His
435 440 445
Gln Gly Ala Ile Asp Gln Asp Asn Leu Leu Glu Phe Val Asp Tyr Leu
450 455 460
Val Asn Ser Leu Asp Cys Lys Ile Ile Ile Leu Asp Pro Ile Thr Leu
465 470 475 480
Ala Leu Ser Arg Ser Asp Thr Asp Glu Glu Glu Val Leu Ser Glu Leu
485 490 495
Leu Arg Arg Cys Lys Arg Tyr Gln Tyr Ala Gln Val Asn Val Cys His
500 505 510
Val Arg Lys Ser Ala Gly Gly Gln Lys Ala Asn Ser Glu Gly Gly Asp
515 520 525
Ile Ser Glu Glu Asp Ile Lys Gly Ser Gly Ala Tyr Phe Gln Ile Ser
530 535 540
Met Asn Asn Ile Leu Leu Met Arg Asn Lys Val Asp Pro Asp Pro Val
545 550 555 560
Lys Lys Asn Leu Thr Lys Ile Lys Leu Thr Lys Cys Arg Arg His Gly
565 570 575
Lys Ser Thr Gly Ile Ala Gly His Thr Trp Tyr Asn Pro Asp Thr Gly
580 585 590
Arg Leu Ile Lys Ala Ser Gly Cys Gly Val Asp Ile Asp Gly Ala Ala
595 600 605
Glu Asn Ile Arg Gln Gln Phe Gly Ile Gly Glu Ala Glu Asp His Tyr
610 615 620
Asp Asp Ser Leu Pro His Tyr Glu Asp Glu Val Phe Asp Arg Glu Thr
625 630 635 640
Gly Glu Val Tyr Thr Glu Glu Gln Arg Gln Ser Ser Thr Ile Pro Pro
645 650 655
Val Leu Ser Glu Asp Ala Asp Asp Cys Pro Phe Glu Thr Glu
660 665 670
<210> 95
<211> 661
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 95
Met Ser Lys Gly Arg Lys Leu Lys Glu Ala Gly Gln Phe Val Gly His
1 5 10 15
Cys Ala Cys Pro Arg Cys Gly Ser Ser Asp Ala Gly Ser Ile Tyr His
20 25 30
His Asp Asp Asp Ser Tyr Ser Met Thr Cys Phe Ser Cys Asn Lys Gly
35 40 45
Phe Pro Glu Trp Asp Phe Asp Lys Gly Gln Ile Val Ser Thr Tyr Ser
50 55 60
Thr Gly Ser Asp Asn Lys Asn Arg Thr Phe Arg Gly Met Asp Leu Asp
65 70 75 80
Asp Val Lys Asp Asn Leu Glu Ala Met Asp Leu Lys Asp Arg Lys Ile
85 90 95
Pro Ala Lys Val Leu Glu Arg Leu Gly Ile Lys Val Asp Ile Asp Ser
100 105 110
Asp Gly Glu Ile Asp Ala His Phe Tyr Pro Thr Tyr Lys Arg Asn Glu
115 120 125
Asp Gly Lys Leu Glu His Val Gly Tyr Arg Val Arg His Arg Tyr Pro
130 135 140
Glu Asp His Pro Lys Glu His Leu Arg Gly Lys Leu Lys Asp Phe Ser
145 150 155 160
Gly Gly Val Gly Asp Ile Lys Gly Glu Leu Ala Met Phe Gly Ser Trp
165 170 175
Ile Ala Pro Glu Gly Gly Asn Arg Leu Phe Ile Trp Glu Gly Glu Met
180 185 190
Glu Cys Ala Thr Ala Ile Tyr Met Thr Ser Leu Ala Ile Lys Asp Lys
195 200 205
Ser Arg Arg Lys Asn Tyr Cys His Val Ser Val Pro Ser Gly Ala Asn
210 215 220
Ile Lys Ser Ile Lys Asp Asn Tyr Gln Tyr Ile Thr Ser Phe Asp Glu
225 230 235 240
Ile Tyr Leu Cys Phe Asp Asn Asp Glu Ala Gly Ala Lys Ala Thr Lys
245 250 255
Glu Ala Ala Gly Ile Leu Pro Ile Glu Lys Val Arg Leu Phe Gln Tyr
260 265 270
Pro Glu Gly Val Lys Asp Leu Asn Glu Trp Trp Thr Lys Phe Tyr Lys
275 280 285
Glu Lys Asp Thr Val Leu Glu Gly Phe Lys Gln Arg Ile Tyr Asn Ala
290 295 300
Pro Arg Tyr Cys Pro Ala Gly Ile Lys Asn Phe Ala Asp Gly Phe Glu
305 310 315 320
Ala Met Lys Asn Arg Gly Gln Ile Pro Leu Ile Pro Phe Pro Glu Ser
325 330 335
Phe Gly Asp Leu Asn Arg Leu Thr Tyr Gly Gly Tyr Gly Leu Gly Glu
340 345 350
Ile Thr Thr Ile Ala Ala Pro Ser Ser Val Gly Lys Ser Ala Tyr Thr
355 360 365
Arg Glu Met Ile Tyr Ser Ala Trp Lys Glu Thr Asp Tyr Asn Ile Gly
370 375 380
Val Ile Pro Val Glu Asp Thr Tyr Glu Glu Leu Met Glu Met Leu Cys
385 390 395 400
Ala Ile His Leu Ser Lys Gln Ile Ser Glu Ile Pro Tyr Asp Glu Arg
405 410 415
Asp Trp Asp Glu Leu Lys Gly Ala His Ala Glu Leu Ser Lys Gly Arg
420 425 430
Arg Ile His Ile Val Asp His Gln Gly Ala Ile Asp Gln Asp Asn Leu
435 440 445
Leu Glu Phe Val Asp Tyr Leu Val Asn Ser Leu Asp Cys Lys Ile Ile
450 455 460
Ile Leu Asp Pro Ile Thr Leu Ala Leu Ser Arg Ser Asp Thr Asp Glu
465 470 475 480
Glu Glu Val Leu Ser Glu Leu Leu Arg Arg Cys Lys Arg Tyr Gln Tyr
485 490 495
Ala Gln Val Asn Val Cys His Val Arg Lys Ser Ala Gly Gly Gln Lys
500 505 510
Ala Asn Ser Glu Gly Gly Asp Ile Ser Glu Glu Asp Ile Lys Gly Ser
515 520 525
Gly Ala Tyr Phe Gln Ile Ser Met Asn Asn Ile Leu Leu Met Arg Asn
530 535 540
Lys Val Asp Pro Asp Pro Val Lys Lys Asn Leu Thr Lys Ile Lys Leu
545 550 555 560
Thr Lys Cys Arg Arg His Gly Lys Ser Thr Gly Ile Ala Gly His Thr
565 570 575
Trp Tyr Asn Pro Asp Thr Gly Arg Leu Ile Lys Ala Ser Gly Cys Gly
580 585 590
Val Asp Ile Asp Gly Ala Ala Glu Asn Ile Arg Gln Gln Phe Gly Ile
595 600 605
Gly Glu Ala Glu Asp His Tyr Asp Asp Ser Leu Pro His Tyr Glu Asp
610 615 620
Glu Val Phe Asp Arg Glu Thr Gly Glu Val Tyr Thr Glu Glu Gln Arg
625 630 635 640
Gln Ser Ser Thr Ile Pro Pro Val Leu Ser Glu Asp Ala Asp Asp Cys
645 650 655
Pro Phe Glu Thr Glu
660
<210> 96
<211> 95
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 96
Met Gln Met Thr Ala Arg Leu Lys Leu Ser Asp Ser Tyr Lys Arg Glu
1 5 10 15
Gly Phe Ile Pro Phe Phe Glu Glu Lys Ser Leu Asn Glu Phe Gln Glu
20 25 30
Glu Cys Leu Thr Lys Val Asp Arg Phe Tyr Arg Met Leu Tyr Met Lys
35 40 45
Cys Asp Gly Gly Val Glu Val Glu Glu Lys Phe His His Ser Met Ile
50 55 60
Thr Val Thr Ile Thr Ile Pro Arg His Asn Ile Gly Trp Leu Phe Ile
65 70 75 80
Ile Lys Glu Asn Val Phe Glu Tyr Gln Val Tyr Arg Arg Ile Ser
85 90 95
<210> 97
<211> 49
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 97
Met Lys His Ser Lys Ala Phe Glu Gln Val Phe Gly Asp Ser Leu Lys
1 5 10 15
Ala Thr Ala Gly Lys Pro Ala Lys Tyr Tyr Glu Glu Lys Arg Val Arg
20 25 30
Thr Gly Lys Thr Ala Arg Lys Ala Ala Ser Lys Asp Lys His Asn Phe
35 40 45
Gln
<210> 98
<211> 285
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 98
Met Ser Lys Val Val Lys Met Lys Ala Pro Val Glu Lys Tyr Asn Gly
1 5 10 15
Thr Glu Arg Gln Thr Leu Arg Tyr Leu Leu Lys Asp Val Trp Phe Tyr
20 25 30
Tyr Leu Asn Thr Ser Pro Arg Pro Gly Lys Gly Lys Ser Ile Asp Lys
35 40 45
Lys Phe Pro Gly Lys Asp Cys Asn Tyr Ser Val Ser Ile Leu Ala Glu
50 55 60
Asp Gly Asn Lys Leu Phe Lys Glu Phe Thr Lys Ser Lys Lys Asn Pro
65 70 75 80
Glu Gly Trp Asp Lys Val Thr Thr Glu Ala Val Asp Ala Asp Asp Phe
85 90 95
Glu Glu Lys Phe Gly Cys Lys Pro Pro Phe Glu Ala Asp Thr Tyr Tyr
100 105 110
Ile Leu Lys Val Ser Arg Ala Ala Ala Tyr Lys Asp Gly Ala Val Trp
115 120 125
Thr Ala Lys Gln Ser Phe Pro Val Met Leu Ile Glu Glu Val Asn Gly
130 135 140
Lys Arg Val Ala Val Lys Gln Pro Met Lys Lys Ile Lys Ala Gln Ala
145 150 155 160
Ser Asp Lys His Glu Asp Asp Lys Asn Tyr Asp Val Ile His Pro Asp
165 170 175
Ile Ala Val Gly Asn Gly Ser Phe Gly Ser Val Ile Leu Ser Thr His
180 185 190
Phe Tyr Thr Phe Glu Asn Asn Val Leu Thr Lys Pro Ile Gln Glu Gln
195 200 205
Phe Ile Ile Asp Thr Leu Val Pro Tyr Thr Gly Gly Asn Gly Ala Asn
210 215 220
Gly Glu Pro Glu Leu Asp Glu Asp Glu Leu Ala Met Leu Gly Leu Asp
225 230 235 240
Gly Val Glu Asp Asn Gly Glu Ile Thr Glu Glu Asp Ala Thr Asp His
245 250 255
Lys Pro Ser Asn Asn Ser Asp Asp Gly Asp Asp Glu Asp Leu Pro Asp
260 265 270
Pro Asp Asp Glu Glu Asp Glu Asp Phe Asp Thr Glu Asp
275 280 285
<210> 99
<211> 359
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 99
Leu Tyr Leu Val Trp Gly Phe Phe Ile Trp Arg Val Thr Met Glu Lys
1 5 10 15
Tyr Thr Leu Thr Lys Leu Pro Asp Ser Val Thr His Val Phe Ile Asp
20 25 30
Ser Asp Ser Ile Ala Tyr Lys Gly Ala Cys Val Val Glu Lys Ala Lys
35 40 45
Tyr Lys Tyr Val Asn Lys Leu Thr Ala Glu Glu Ser Glu Pro Phe Asp
50 55 60
Asn Ala Lys Asp Ala Ala Arg Trp Leu Ala Asp Gln Arg Ile Leu Val
65 70 75 80
Glu Glu Leu Gly Leu Thr Phe Asp Glu Asp Glu Trp Glu Arg Gln Thr
85 90 95
Trp Lys Glu Ala Lys Ser Glu Lys Glu Ala Ile Met Ala Thr Gln Gln
100 105 110
Val Leu Gln Glu Trp Leu Lys Val Val Gly Lys Glu Arg Thr Trp Val
115 120 125
Gly Tyr Leu Thr Glu Lys Gly Val His Lys His Lys Asp Ile Lys Gly
130 135 140
Leu Glu His Gln Tyr Gln Gly Asn Arg Lys Asp Ala Val Thr Pro Thr
145 150 155 160
His Leu Val Ala Cys Arg Glu Tyr Leu Leu Ser Arg Pro Glu Phe Lys
165 170 175
Leu Leu Lys Gly Gly Phe Glu Ala Asp Ser Ile Val Ile Ala Lys Ala
180 185 190
Glu Lys Met Gly Lys Lys Ala Ala Leu Met Ser Ile Asp Lys Asp Leu
195 200 205
Arg Gln Ala Glu Gly Thr Tyr Cys Ile Asp Met Thr Tyr Glu Lys Ser
210 215 220
Pro Leu Ile Phe Ile Ala Asp Asn Asn Val Gly Asp Ile Trp Asp Cys
225 230 235 240
Pro Ile Lys Ser Lys Pro Lys Ala Ala Lys Thr Val Gly Val Gly Phe
245 250 255
Lys Phe Leu Cys Tyr Gln Ala Val Ala Gly Asp Asn Ala Asp Asn Tyr
260 265 270
Phe Gly Leu Lys Gly Ala Gly Lys Val Ala Val Met Asn Ala Leu Asp
275 280 285
Gly Lys Glu Thr Tyr Lys Glu Cys Leu Asp Ala Ile Tyr Glu Leu Tyr
290 295 300
Ala Lys Lys Glu Ser Tyr Thr Tyr Val Ser Trp Asp Gly Gln Thr Val
305 310 315 320
Thr Lys Thr Pro Leu Glu Leu Met Glu Gln His Phe Trp Val Ala Tyr
325 330 335
Gln Glu Arg Asn Pro Lys Asp Asn Phe Ser Phe Lys Lys Tyr Asn Trp
340 345 350
Glu Pro Cys Met Asn Glu Asp
355
<210> 100
<211> 165
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 100
Met Lys Ile Lys Gly Phe Gly Asn Cys Pro Thr Tyr Gly His Trp Val
1 5 10 15
Ser Leu Cys Gly Glu Val Asp Pro Thr Lys His Phe Gly Phe Val Tyr
20 25 30
Leu Val Tyr Cys Lys Lys Thr Gly Gln Tyr Tyr Ile Gly Lys Lys Gln
35 40 45
Leu Asn Ser Val Thr Lys Arg Lys Val Ala Gly Lys Thr Arg Lys Lys
50 55 60
Val Val Thr Lys Glu Ser Asp Trp Met Thr Tyr Glu Thr Ser Ser Glu
65 70 75 80
Tyr Ile Lys Lys Asp Ile Glu Ser Phe Gly Lys Glu Phe Phe Asp Phe
85 90 95
Tyr Ile Ile Gln Thr Tyr His Thr Lys Gly Gly Leu Val Tyr Gly Glu
100 105 110
Ala Asn Leu Gln His Lys Phe Asp Val Met Thr Lys Arg Ile Asp Ser
115 120 125
Lys Leu Arg Leu Phe Tyr Asn Ala Asn Ile Ala Ala Ile Lys Phe Ile
130 135 140
Thr Lys Glu Thr Tyr Glu Asp Ala Glu Lys Arg Ile His Lys Val Met
145 150 155 160
Lys Ala Asn Cys Ala
165
<210> 101
<211> 744
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 101
Met Ile Lys Thr Ile Lys Lys Ser Asn Gly Thr Val Val Ser Phe Asp
1 5 10 15
Pro Glu Arg Leu Asn Lys Trp Ala Ser Trp Ala Asp Lys Arg Gly Ile
20 25 30
Ile Trp Ser Glu Val Thr Met Glu Ala Met Lys Arg Val Tyr Gly Gly
35 40 45
Cys Thr Thr Lys Glu Met His Gln Ala Met Ile Asp Val Cys Val Asp
50 55 60
Lys Gln Thr Gln Glu Tyr Ser Asp Met Ala Gly Arg Leu Leu Leu Gly
65 70 75 80
Ile Ile Tyr Lys Glu Ala Phe Gly Gly Phe Thr Lys Val Pro Thr Leu
85 90 95
Val Thr Phe Val Lys Asn Met Glu Arg Ala Gly Leu Trp Glu Lys Met
100 105 110
Asp Tyr Ser Gln Glu Glu Leu Glu Tyr Leu Gln Gly Tyr Ile Val His
115 120 125
Ser Lys Asp Ile Ser Tyr Gly Tyr Ala Val Leu Lys Gln Phe Arg Asp
130 135 140
Lys Tyr Gly Ile Arg Asp Ile Lys Thr Gly Arg Leu Phe Glu Ser Pro
145 150 155 160
Gln Phe Met Phe Met Gly Met Ala Met Lys Ala Phe Glu Lys Gln Pro
165 170 175
Lys His Arg Arg Leu Gln Asp Val Ile Lys Leu Tyr Thr Tyr Leu Ser
180 185 190
Asp Leu Lys Ile Asn Ala Pro Thr Pro Tyr Leu Asn Gly Leu Arg Ala
195 200 205
Thr Lys Ser Gly Tyr Ala Ser Cys Cys Leu Ile Lys Ala Asn Asp Thr
210 215 220
Ala Glu Ser Leu Gly Ile Ala Ala Lys Val Ala Tyr Asp Met Thr Thr
225 230 235 240
Lys Gln Ala Gly Ile Gly Met Leu Met Glu Thr Arg Thr Ile Gly Asp
245 250 255
Gly Ile Arg Gln Asn Thr Ile Glu His Met Gly Lys Leu Pro Tyr Tyr
260 265 270
Lys Leu Val Arg Ser Ser Val Glu Ala Asn Lys Gln Lys Ser Arg Gly
275 280 285
Gly Ser Ala Asn Asn Phe Tyr Thr Ala Leu Asp Pro Gln Ile Glu Asp
290 295 300
Leu Leu Arg Leu Lys His Pro Thr Thr Val Pro Ser Lys Arg Ile Asn
305 310 315 320
Glu Met Asp Tyr Ser Phe Gly Thr Asn Asp Tyr Phe Trp Gln Cys Val
325 330 335
Gln Tyr Asp Thr Asp Trp Leu Leu Phe Ser Tyr Lys Asp Ala Pro Lys
340 345 350
Leu Tyr Asp Met Phe Tyr Thr Ala Ser Ala Asp Glu Phe Ala Met Ala
355 360 365
Val Gly His Ala Val His Ser Gly Val Lys His Arg Arg Val Lys Ala
370 375 380
Arg Glu Ile Ala Lys Leu Phe Ile Gln Gln Arg Tyr Ala Thr Gly Arg
385 390 395 400
Val Tyr Pro Phe Phe Thr Asn Asn Ala Asn Thr His Thr Pro Phe Lys
405 410 415
Glu Pro Leu Lys Met Ser Asn Leu Cys Met Glu Ile Val Leu Pro Val
420 425 430
Tyr Gly Phe Glu Lys Glu Thr Asp Leu Tyr Arg Asp Asp Ala Val Lys
435 440 445
Glu Asp Gly Glu Val Ala Leu Cys Phe Leu Ala Ser Leu Val Ala Gly
450 455 460
Arg Ile Ser Glu Asp Glu Tyr Ala Asp Val Ala Tyr Tyr Ala Leu Ala
465 470 475 480
Met Val Asp Ser Val Ile Asp Leu Met Asp Tyr Pro Tyr Pro Ser Met
485 490 495
Arg Asn His Val Gln Lys Arg Arg Ser Val Gly Ile Gly Leu Thr Asn
500 505 510
Val Ala His Tyr Leu Ala Lys Asn Tyr Val Asn Tyr Ser Ser Arg Ala
515 520 525
Gly Lys Thr Lys Leu His Glu Leu Ala Glu Met His Ser Tyr Tyr Leu
530 535 540
His Glu Ala Ser Leu Arg Leu Ala Lys Glu Arg Gly Val Pro Glu Tyr
545 550 555 560
Met Lys Phe Thr Lys Tyr Pro Glu Gly Trp Val Pro Pro Lys Thr Ala
565 570 575
Asn Lys Lys Ile Asp Glu Lys His Asp Ala Lys Leu Arg Tyr Asp Trp
580 585 590
Asp Asp Leu Ala Gln Arg Ile Lys Glu Asn Gly Gly Ile Arg Asn Ser
595 600 605
Val Leu Glu Ala Tyr Met Pro Asn Glu Ser Ser Ser Leu Ala Thr Asn
610 615 620
Thr Thr Asn Gly Leu Tyr Pro Ile Arg Asp Phe Ile Leu Thr Lys Lys
625 630 635 640
Ser Ala Thr Gly Asn Val Leu Phe Ile Val Pro Asp Tyr Glu Glu Leu
645 650 655
Lys Tyr Val Tyr Glu Ile Ala Trp Gly Ile Asp Thr Phe Asp Leu Ile
660 665 670
Asp Cys Tyr Ala Ile Val Gln Lys Phe Thr Gly Gln Ala Ile Ser Ser
675 680 685
Asp Phe Tyr Val Asp Tyr Ala Lys Ala Lys Lys Val Ser Leu Ala Gln
690 695 700
Ala Leu Lys Tyr Met Ile Tyr Ala Asn Ser Val Gly Met Lys Thr Met
705 710 715 720
Tyr Tyr Leu Asn Ser Arg Ile Gly Val Gly Lys Ser Ala Leu Gln Asp
725 730 735
Ala Tyr Cys Glu Gly Cys Gly Val
740
<210> 102
<211> 377
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 102
Leu Gly Ser Phe Arg Gln Lys Thr Ser Leu Arg Asn Asp Lys Glu Arg
1 5 10 15
Arg Asn His Ala Met Ile Asn Gln His Pro Ile Phe Leu Gly Gly Glu
20 25 30
Arg Lys Thr Phe Asp Ser Leu Asn Lys His Tyr Pro Lys Ile Phe Glu
35 40 45
Leu Tyr Lys Gln Gln Lys Ala Gln Asp Trp Ser Glu Asp Glu Phe Pro
50 55 60
Phe Glu Gln Ser Arg Leu Asp Phe Glu Ser Val Pro Ala Ser Met Ser
65 70 75 80
Gly Val Met Leu Glu Ile Leu Lys Trp Gln Trp Glu Ala Asp Thr Gln
85 90 95
Val Ala Lys Ser Leu Ala Phe Ala Phe Ala Pro Phe Ile Ser Asp Asp
100 105 110
Ile Tyr Ala Thr Ala Ile Met Lys Gln Ser Glu Ile Glu Asn Leu His
115 120 125
Ala Leu Thr Tyr Ser Glu Ile Val Arg Gln Cys Ile Lys Asn Pro Glu
130 135 140
Thr Ile Leu Asp Glu Ile Asn Gln Asn Val Ala Val Gln Asp Arg Leu
145 150 155 160
Lys Thr Val Asn Arg Val Leu Glu Glu Leu Leu Asp Glu Gly Ile Asn
165 170 175
Tyr Arg Leu Ser Tyr Val Arg Asp Ser Leu Leu Asp Lys Asp Pro Leu
180 185 190
His Phe His Lys Val Ile Leu Lys Gly Leu Phe Ala Val Thr Ala Leu
195 200 205
Glu Gly Ile Ser Phe Met Ala Ser Phe Ala Cys Thr Phe Ala Leu Asp
210 215 220
Ala Gln Asp Lys Phe Gln Gly Ile Ala Gln Ala Val Gln Lys Ile Met
225 230 235 240
Leu Asp Glu Ile Leu His Thr Lys Ile Asp Ile Glu Val Leu Lys Glu
245 250 255
Thr Leu Arg Asp Asp Glu Trp Gln Lys Ala Phe Gln Gln Ile Leu Pro
260 265 270
Glu Ile Lys Val Ile Leu Asp Glu Val Val Glu Ser Glu Glu Lys Trp
275 280 285
Ser Tyr Tyr Ile Phe Ser Glu Gly Arg Ala Val Val Gly Leu Asn Thr
290 295 300
Lys Leu Leu His Glu Trp Val Tyr Tyr Asn Ala Ala Pro Leu Tyr Asp
305 310 315 320
Met Phe Gly Ile Pro Arg Asp Phe Val Ala Pro Lys Glu Pro Pro Leu
325 330 335
Lys Tyr Met Ile Lys Lys Met Glu Ile Asp Lys Glu Gln Asn Ala Asn
340 345 350
Gln Glu Gln Gln Asn Gly Ala Tyr Leu Leu Asn Thr Val Val Asp Asp
355 360 365
Leu Asn Ser Gly Phe Leu Glu Val Pro
370 375
<210> 103
<211> 80
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 103
Met Thr Tyr Val Ile Tyr Ser Lys Thr Gly Cys Pro Gln Cys Glu Thr
1 5 10 15
Ala Lys Asn Phe Ala Lys Ala Arg Gly Ile Asp His Val Val Arg Met
20 25 30
Leu Gly Gln Asp Tyr Glu Leu Ser Asp Leu Met Glu Ile Ala Gln Met
35 40 45
Pro Val Arg Gln Met Pro Phe Ile Met Lys Thr Asp Gly Gln Asn Leu
50 55 60
Lys Pro Val Gly Thr Leu Gln Asn Phe Met Ala Glu Val Asn Asn Ala
65 70 75 80
<210> 104
<211> 730
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 104
Asn Glu Arg Val Ser Ser Glu Thr Phe Leu Ala Cys Lys Gly Ala Ile
1 5 10 15
Met Asn Lys Val Gln Ile Ile Lys Lys Asn Gly Ser Leu Glu Glu Pro
20 25 30
Asp Ile Lys Lys Val Leu Ala Ala Val Thr Lys Ser Ala Asn Arg Val
35 40 45
Gly Tyr Lys Glu Leu Pro Pro Asp Val Thr Gln Ala Leu Glu Ser Ala
50 55 60
Phe Met Arg Ile Leu Val Lys Ser Thr Lys Gln Asn Asn Leu Leu Ile
65 70 75 80
Ser Val Asn Asp Ile His Ser Ile Val Glu Gly Ala Leu Ala Glu Val
85 90 95
Asn His Glu Ile Tyr Glu Ser Tyr Ser Thr Tyr Arg Asn Tyr Arg Lys
100 105 110
Glu Val Ala Gln Asn Trp Asp Glu Leu Tyr Gln Lys Thr Lys Asp Thr
115 120 125
Leu Phe Leu Gly Asp Arg Glu Asn Ala Asn Phe Asp Ser Ser Leu Ile
130 135 140
Ser Thr Lys Gly Ser Ile Ile Arg Gly Tyr Leu Thr Lys Glu Ile Phe
145 150 155 160
Lys Gln Tyr His Leu Thr Pro Glu Glu Leu Glu Ala Ile Glu Lys Gly
165 170 175
Phe Ile Tyr Ile His Asp Leu Arg Asp Leu Ile Phe Gly Gly Ile Asn
180 185 190
Cys Cys Leu Phe Asp Ile Gly Lys Val Leu Lys Gly Gly Phe Glu Met
195 200 205
Ser Gly Ile Glu Tyr Cys Glu Pro Lys Ser Val Leu Ser Ala Leu Gln
210 215 220
Val Ile Gly Asp Val Val Leu Ser Ala Thr Ala Gln Gln Phe Gly Gly
225 230 235 240
Phe Thr Leu Ala Glu Ile Asp Lys Val Leu Val Pro Tyr Ala Lys Lys
245 250 255
Ser Leu Arg Tyr His Ala Glu Lys Ala Ala Ser Tyr Gly Ile Pro Lys
260 265 270
Glu His Tyr His Asn Tyr Val Met Glu Gln Leu Gln Ile Glu Leu Thr
275 280 285
Gln Gly Phe Gln Ser Leu Glu Met Lys Leu Asn Thr Val Pro Cys Ser
290 295 300
Arg Gly Asp Phe Ala Phe Thr Thr Leu Thr Phe Gly Leu Leu Asp Ser
305 310 315 320
Asp Met Ser Asn Glu Asp Asn Arg Leu Gln Tyr Met Ile Ala Ser Thr
325 330 335
Ile Leu Asp Val Arg Met Asn Gly Gln Gly Lys Ser Lys Lys Pro Val
340 345 350
Val Phe Pro Lys Leu Val Tyr Ile Tyr Asp Gln Lys Arg His Asn Glu
355 360 365
Asn Ile Cys Gln Gly His Leu Tyr Ser Lys Ala Ile Glu Cys Cys Ser
370 375 380
Lys Ala Met Tyr Pro Asp Phe Leu Ser Val Ser Gly His Gly Ala Val
385 390 395 400
Ala Glu Ala Phe Glu Arg Ser Gly Lys Val Ile Ser Pro Met Gly Cys
405 410 415
Arg Ala Phe Leu Ser Pro Tyr Leu Asn Glu Asp Gly Glu Glu Phe Tyr
420 425 430
Val Gly Arg Ala Asn Ile Gly Ala Val Ser Leu Asn Leu Pro Met Ile
435 440 445
Tyr Gln Tyr Ser Lys Glu Asn Gly Leu Asp Phe Trp Lys Glu Leu Asp
450 455 460
Lys Tyr Leu Glu Met Ile Arg Ser Phe His Lys Lys Arg Tyr Glu Met
465 470 475 480
Ile Ala Asn Met Pro Ala Ser Ser Asn Pro Leu Ala Phe Thr Gln Gly
485 490 495
Gly Leu Tyr Lys Gly Thr Lys Lys Pro Thr Asp Arg Val Gly Trp Asp
500 505 510
Ile Val Lys Ser Phe Thr Ala Ser Phe Gly Val Thr Ala Leu Asp Glu
515 520 525
Leu Ser Val Leu Ala Glu Gly Lys Arg Leu His Glu Val Gly Ser Cys
530 535 540
Ser Phe Ala Tyr Asp Val Leu Glu Tyr Ile Asn Met Lys Thr Glu Glu
545 550 555 560
Phe Lys Asn Glu Asp Gly Phe Leu Tyr Ala Val Tyr Gly Thr Pro Ala
565 570 575
Glu Ser Leu Cys Gly Thr Gln Leu Lys Gln Phe Arg Glu Met Phe Gly
580 585 590
Val Ser Lys Gly Val Ser Asp Lys Glu Tyr Phe Thr Asn Ser Phe His
595 600 605
Met Asn Val Ala Ala Asp Ile Thr Pro Phe Glu Lys Gln Asp Leu Glu
610 615 620
Glu Pro Phe Phe His Ile Cys Arg Gly Gly Arg Ile Gln Tyr Val Arg
625 630 635 640
Val Ala Asn Pro Glu Asn Leu Ser Ala Leu Lys Ser Cys Ile Thr Arg
645 650 655
Gly Met Leu Lys Gly Phe Tyr Gln Gly Leu Asn Phe Asp Leu Ala Ile
660 665 670
Cys Glu His Cys Gly Asn Arg Pro Lys Ala Asp Val Glu Glu Cys Glu
675 680 685
Val Cys His Ser His Asp Ile Ser Val Ile Asn Arg Val Cys Gly Tyr
690 695 700
Leu Gly Trp Thr Lys Ile Lys Gly Glu Ser Arg Met Asn Asp Ala Lys
705 710 715 720
Ile Ala Glu Ile Arg Asp Arg Ile Ser Met
725 730
<210> 105
<211> 714
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 105
Met Asn Lys Val Gln Ile Ile Lys Lys Asn Gly Ser Leu Glu Glu Pro
1 5 10 15
Asp Ile Lys Lys Val Leu Ala Ala Val Thr Lys Ser Ala Asn Arg Val
20 25 30
Gly Tyr Lys Glu Leu Pro Pro Asp Val Thr Gln Ala Leu Glu Ser Ala
35 40 45
Phe Met Arg Ile Leu Val Lys Ser Thr Lys Gln Asn Asn Leu Leu Ile
50 55 60
Ser Val Asn Asp Ile His Ser Ile Val Glu Gly Ala Leu Ala Glu Val
65 70 75 80
Asn His Glu Ile Tyr Glu Ser Tyr Ser Thr Tyr Arg Asn Tyr Arg Lys
85 90 95
Glu Val Ala Gln Asn Trp Asp Glu Leu Tyr Gln Lys Thr Lys Asp Thr
100 105 110
Leu Phe Leu Gly Asp Arg Glu Asn Ala Asn Phe Asp Ser Ser Leu Ile
115 120 125
Ser Thr Lys Gly Ser Ile Ile Arg Gly Tyr Leu Thr Lys Glu Ile Phe
130 135 140
Lys Gln Tyr His Leu Thr Pro Glu Glu Leu Glu Ala Ile Glu Lys Gly
145 150 155 160
Phe Ile Tyr Ile His Asp Leu Arg Asp Leu Ile Phe Gly Gly Ile Asn
165 170 175
Cys Cys Leu Phe Asp Ile Gly Lys Val Leu Lys Gly Gly Phe Glu Met
180 185 190
Ser Gly Ile Glu Tyr Cys Glu Pro Lys Ser Val Leu Ser Ala Leu Gln
195 200 205
Val Ile Gly Asp Val Val Leu Ser Ala Thr Ala Gln Gln Phe Gly Gly
210 215 220
Phe Thr Leu Ala Glu Ile Asp Lys Val Leu Val Pro Tyr Ala Lys Lys
225 230 235 240
Ser Leu Arg Tyr His Ala Glu Lys Ala Ala Ser Tyr Gly Ile Pro Lys
245 250 255
Glu His Tyr His Asn Tyr Val Met Glu Gln Leu Gln Ile Glu Leu Thr
260 265 270
Gln Gly Phe Gln Ser Leu Glu Met Lys Leu Asn Thr Val Pro Cys Ser
275 280 285
Arg Gly Asp Phe Ala Phe Thr Thr Leu Thr Phe Gly Leu Leu Asp Ser
290 295 300
Asp Met Ser Asn Glu Asp Asn Arg Leu Gln Tyr Met Ile Ala Ser Thr
305 310 315 320
Ile Leu Asp Val Arg Met Asn Gly Gln Gly Lys Ser Lys Lys Pro Val
325 330 335
Val Phe Pro Lys Leu Val Tyr Ile Tyr Asp Gln Lys Arg His Asn Glu
340 345 350
Asn Ile Cys Gln Gly His Leu Tyr Ser Lys Ala Ile Glu Cys Cys Ser
355 360 365
Lys Ala Met Tyr Pro Asp Phe Leu Ser Val Ser Gly His Gly Ala Val
370 375 380
Ala Glu Ala Phe Glu Arg Ser Gly Lys Val Ile Ser Pro Met Gly Cys
385 390 395 400
Arg Ala Phe Leu Ser Pro Tyr Leu Asn Glu Asp Gly Glu Glu Phe Tyr
405 410 415
Val Gly Arg Ala Asn Ile Gly Ala Val Ser Leu Asn Leu Pro Met Ile
420 425 430
Tyr Gln Tyr Ser Lys Glu Asn Gly Leu Asp Phe Trp Lys Glu Leu Asp
435 440 445
Lys Tyr Leu Glu Met Ile Arg Ser Phe His Lys Lys Arg Tyr Glu Met
450 455 460
Ile Ala Asn Met Pro Ala Ser Ser Asn Pro Leu Ala Phe Thr Gln Gly
465 470 475 480
Gly Leu Tyr Lys Gly Thr Lys Lys Pro Thr Asp Arg Val Gly Trp Asp
485 490 495
Ile Val Lys Ser Phe Thr Ala Ser Phe Gly Val Thr Ala Leu Asp Glu
500 505 510
Leu Ser Val Leu Ala Glu Gly Lys Arg Leu His Glu Val Gly Ser Cys
515 520 525
Ser Phe Ala Tyr Asp Val Leu Glu Tyr Ile Asn Met Lys Thr Glu Glu
530 535 540
Phe Lys Asn Glu Asp Gly Phe Leu Tyr Ala Val Tyr Gly Thr Pro Ala
545 550 555 560
Glu Ser Leu Cys Gly Thr Gln Leu Lys Gln Phe Arg Glu Met Phe Gly
565 570 575
Val Ser Lys Gly Val Ser Asp Lys Glu Tyr Phe Thr Asn Ser Phe His
580 585 590
Met Asn Val Ala Ala Asp Ile Thr Pro Phe Glu Lys Gln Asp Leu Glu
595 600 605
Glu Pro Phe Phe His Ile Cys Arg Gly Gly Arg Ile Gln Tyr Val Arg
610 615 620
Val Ala Asn Pro Glu Asn Leu Ser Ala Leu Lys Ser Cys Ile Thr Arg
625 630 635 640
Gly Met Leu Lys Gly Phe Tyr Gln Gly Leu Asn Phe Asp Leu Ala Ile
645 650 655
Cys Glu His Cys Gly Asn Arg Pro Lys Ala Asp Val Glu Glu Cys Glu
660 665 670
Val Cys His Ser His Asp Ile Ser Val Ile Asn Arg Val Cys Gly Tyr
675 680 685
Leu Gly Trp Thr Lys Ile Lys Gly Glu Ser Arg Met Asn Asp Ala Lys
690 695 700
Ile Ala Glu Ile Arg Asp Arg Ile Ser Met
705 710
<210> 106
<211> 43
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 106
Met Val Trp Gly Phe Ser Asp Pro Gln Trp Ser Phe Ser Tyr His Gly
1 5 10 15
Gln Met Asp Leu Trp Ser Cys Phe Lys Pro Phe Leu Gly Leu Ala Ile
20 25 30
Ala Phe Ser Ala Leu Pro Thr Lys Val Lys Leu
35 40
<210> 107
<211> 131
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 107
Met Ile Lys Leu Ser Gln Lys Gln Leu Glu Trp Ile Lys Asp Tyr Ala
1 5 10 15
Ser Glu Cys Gly Ser Cys Glu Lys Asn His Val Arg Tyr Ser Thr Phe
20 25 30
His Thr Val Phe Thr Leu Tyr Val Gly Asp Asn Val Leu Ser Asp Ser
35 40 45
Val Val Asp Gly Val Pro Leu Pro Asn Glu Leu Leu Asp Lys Leu Ala
50 55 60
Val Val Thr Gly Thr Trp Ser Glu Glu Asp Gly His Asp Leu Ser Asn
65 70 75 80
Asp Val Val Phe Tyr Ser Leu Glu Asn Ile Met Asn Pro Glu Tyr Ile
85 90 95
Met Leu Met Thr Cys Ala Gln Asp Cys Val Pro Leu Gln Asn Phe Ile
100 105 110
Lys Glu His Cys Glu Glu Phe Ile Thr Lys Gln Val Pro Cys Gln Val
115 120 125
Val Phe Glu
130
<210> 108
<211> 161
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 108
Met Asn Tyr Met Glu Ile Arg Pro Phe Asp Thr Ala Asn Gly Glu Gly
1 5 10 15
Val Arg Val Ser Leu Phe Val Ala Gly Cys Lys His His Cys Glu Gly
20 25 30
Cys Phe Asn Arg Glu Ser Trp Lys Phe Asn Ala Gly Lys Glu Phe Thr
35 40 45
Tyr Ala Asn Leu Tyr Gly Ile Ile Lys Leu Met Asp Asp Lys Ala Ile
50 55 60
Ser Gly Leu Ser Ile Leu Gly Gly Glu Pro Leu Asp Asp Arg Asn Ile
65 70 75 80
Gln Glu Val Thr Asn Ile Cys Lys Arg Ile Lys Thr Val Tyr Pro Glu
85 90 95
Lys Ser Ile Trp Leu Trp Thr Gly Phe Gln Leu His Glu Lys Ile His
100 105 110
Leu Asp Val Met Lys Tyr Val Asp Val Val Ile Asp Gly Lys Tyr Asp
115 120 125
Ser Ser Lys Pro Thr Val Lys Pro Tyr Arg Gly Ser Asp Asn Gln Asn
130 135 140
Leu Trp Arg Lys Glu Tyr Gly Trp Gln Gly Asp Cys Gln Trp Arg Ala
145 150 155 160
Glu
<210> 109
<211> 38
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 109
Met Cys Tyr Arg Thr Asn Leu Thr Val Gly Val Thr Glu Glu Glu Leu
1 5 10 15
Lys Ala Phe Cys Glu Lys Ile Glu Arg Asp Thr Lys Trp Glu Ser Gly
20 25 30
Thr Val Leu Asn Gln Lys
35
<210> 110
<211> 106
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 110
Met Ser Lys Val Tyr Asn Thr Arg Lys Leu Gln Ile Phe Val Leu Cys
1 5 10 15
Gln Phe Met Ala Lys Glu Tyr Asn Cys Tyr Tyr Cys Gly Thr Gly Phe
20 25 30
Ile Ser Asp Asn Asp Gly Ser Tyr Leu Pro Phe Lys Glu Ala Val Lys
35 40 45
Leu Phe Asn Glu Glu Val Ser Ser Lys Lys Asp Ile Glu Lys Val Lys
50 55 60
Leu Thr Tyr Ser Lys Lys Asp Lys Lys Ile Ile Cys Leu Asp Asn Phe
65 70 75 80
Val Lys Val Thr Gly Glu Ala Lys Glu Phe Met Asp Glu Ser Glu Ile
85 90 95
Ser Phe Thr Ser Ile Leu Lys Val Ala Gln
100 105
<210> 111
<211> 92
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 111
Met Ala Arg Thr Asp Trp Glu Val Lys Glu Leu Val Ser Asp Ala Gly
1 5 10 15
Asn Gly Cys Glu Val Tyr Met Ile Arg Gly His Val Pro Asp Pro Leu
20 25 30
Ala Ile Glu Met Ile Asp His His Thr Ser Gly Phe Tyr Lys Glu Leu
35 40 45
Gly Glu Pro Ser Val Glu Arg Thr Trp Thr Lys Pro Val Pro Asp Ser
50 55 60
Thr Gly Asn Cys Ser Val Ile Tyr His Ile Val Asn Pro Gln Lys Cys
65 70 75 80
Lys Thr Ala Met Ala Val Thr Phe Val Thr Phe Asp
85 90
<210> 112
<211> 298
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 112
Leu Arg Glu Lys Ile Met Lys Thr Ile Ile Glu Ala Val Val Thr Ala
1 5 10 15
Gln Glu Lys Phe Phe His Thr Glu Lys Phe Asn Ile Ile Gln Phe Pro
20 25 30
Ser Gly Glu Ile Gly Gly Asn Phe Ser Glu Asp Phe Val Lys Phe Thr
35 40 45
Glu Arg Asn Ala Gly Lys Ile Asp Asn Val Ile Leu Thr Val Gln Gly
50 55 60
Tyr Asp Lys Asp Thr Leu Phe Ala Leu Ala Leu Ala Lys Asp Ala Val
65 70 75 80
Asp Ser Leu Val Pro Tyr Lys Ser Ala Met Lys Thr Val Val Phe Gly
85 90 95
Phe Leu Pro Asn Ala Arg Tyr Asp Arg His Met Phe Lys Gly Asp Ser
100 105 110
Ala Ala Leu Lys Ala Phe Ala Asn Leu Val Asn Ala Met Gly Phe Asp
115 120 125
Ala Val Cys Ala Leu Asp Pro His Ser Asn Val Ala Glu Asn Leu Phe
130 135 140
Lys Cys Phe Gln Ser Met Lys Gln Lys Asp Val Ala Ala His Phe Ala
145 150 155 160
Ser Asp Pro Arg Ile Asp Phe Leu Val Ala Pro Asp Ala Gly Ala Ala
165 170 175
Lys Lys Thr Glu Glu Thr Ala Lys Glu Val Asp Lys Pro Tyr Ile Thr
180 185 190
Met Ser Lys Val Arg Asn Leu Lys Thr Gly Glu Ile Thr Gly Met Arg
195 200 205
Ile Leu Asp Thr Val Asp Leu Thr Asp Lys Thr Val Met Ile Leu Asp
210 215 220
Asp Ile Cys Asp Gly Gly Arg Thr Phe Ile Glu Ala Ala Lys His Leu
225 230 235 240
Arg Glu Ala Gly Ala Lys Arg Val Glu Leu Tyr Val Thr His Gly Ile
245 250 255
Phe Ser Lys Gly Val Glu Asn Leu Leu Asp Asn Gly Val Asp His Ile
260 265 270
Tyr Thr Thr Asn Ser Leu Gly Glu Ala Lys Asp Arg Gly Leu Thr His
275 280 285
Tyr Thr Gln Val Thr Val Val Thr Ile Asp
290 295
<210> 113
<211> 128
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 113
Pro Gln Ser Met Arg Leu Lys Gln Asn Leu Ser Phe Ile Ile Phe Val
1 5 10 15
Ile Ile Ser Ala Thr Thr Val Ala Cys Ser Phe Asn Lys Trp Val Ser
20 25 30
Phe Asn Leu Ile Met Trp Asn Gly Asp Pro Ser Ile Pro Pro Phe Lys
35 40 45
Glu Ser Ser Lys Thr Leu Asn Val Ser Arg Leu Ala Pro Leu Ile Ser
50 55 60
Leu Thr Ile Asn Val Thr Cys Pro Tyr Ser Leu Ser Val Ser Asn Ala
65 70 75 80
Asn Ala Lys Ala Leu Ser Ala Thr Ser Leu Arg Thr Gly Tyr Leu Asp
85 90 95
Ala Ile Phe Val Thr Thr Lys Pro Asn Lys Gly Phe Ala Arg Tyr Ser
100 105 110
Arg Trp Ile Asn Leu Pro Ser Glu Ala Ala Ser Phe Ala Lys Ala Leu
115 120 125
<210> 114
<211> 556
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 114
Leu Asn His Phe Met Gln Tyr Gln Gln Val Thr Gln Met Leu Thr Asn
1 5 10 15
Leu Val Thr Phe Thr Ser Thr Leu Val Gln Gln Asn Thr Cys Ser Ile
20 25 30
His His Val Val Ile Ser Gly Ser Thr Ala Pro Gln Leu Met Val Ser
35 40 45
Leu Leu Val Phe Asn Val Leu Leu Lys Ile Thr Thr Thr Gly Thr Pro
50 55 60
Pro Ser Ser Asn Val Thr Lys Lys Lys Gln Leu Thr Lys Ser Lys Leu
65 70 75 80
Thr Val Phe Trp Val Lys Met Leu Leu Val Glu Asn Ile Gly Lys His
85 90 95
Ser Thr Thr Val Ile Tyr Gln Leu Lys Ser Thr Leu Lys Lys Ala Gln
100 105 110
Leu Phe Leu Cys Val Tyr Gln Leu Ser Ser Arg Thr Leu Phe Gln Val
115 120 125
Ser Ile Gly Arg Asp Ile Trp Lys Met Leu Ser Leu Leu Arg Phe Gly
130 135 140
Arg Leu Val Pro Leu Gln Leu Leu His Cys Thr Thr Asn Val Ser Val
145 150 155 160
Arg Ser Gly Leu Thr Leu Leu Val Thr Thr Thr Tyr Ile Cys Leu Ile
165 170 175
Ser Ala Met Thr Leu Leu Cys Val Val Cys Pro Ala Leu Leu Met Thr
180 185 190
His Leu Thr Leu Val Thr Gln Ala Leu Lys Gly Leu Ile Ala Ser Leu
195 200 205
Arg Tyr Ile Gln Leu Asn Ala Ser Met Asp Ser Pro Ile Gln Ser Leu
210 215 220
Ile Leu Val Ala Leu Tyr Gln Pro Leu Asn Thr Leu Cys Val Gln Thr
225 230 235 240
Leu Leu Gly Lys Val Val Met Ser Leu Lys Lys Lys Asp Ala Ser Lys
245 250 255
Val Ser Tyr Lys Pro Ser Val Ala Ser Gln Lys Leu Thr Gln Leu Val
260 265 270
Leu Gln Val Leu Phe Gln Thr Leu Ile Ile Ser Gly Glu Leu Tyr Gln
275 280 285
Tyr Tyr Gln Asn Ser Val Lys Arg Leu Trp Asn Val Met Ala Asn Trp
290 295 300
Phe Val Leu Thr Leu Glu Thr Leu Tyr Ile Leu Ser Gln Asp Ile Lys
305 310 315 320
Gln Phe Thr Ser Val Leu Lys Arg Leu Ile Thr Asn Thr Ala Ser Arg
325 330 335
Met Pro Val Thr Gln Cys Ala Pro Ser Thr Ser Leu Lys Ile Leu Thr
340 345 350
Met Leu Trp Leu Gly Gly Tyr Val Gln Lys Ala Met Lys Trp Trp Leu
355 360 365
Thr Glu Lys Thr Leu Lys Pro Ile Gln Cys Cys Lys Met Leu Thr Ser
370 375 380
Thr Ala Leu Gln Thr Leu His Val Leu Arg Arg Leu Met Glu Leu Leu
385 390 395 400
Arg His Cys Thr Thr Ser Leu Gly Glu Leu Leu Thr Leu Lys Gly Leu
405 410 415
Arg Tyr Trp Met Ser Thr Ser Ala Leu Ser Met Val Thr Leu Ser Arg
420 425 430
Trp Asn Val Gln Thr Lys Ser Ser Val Cys Met Lys Trp Val Leu Gln
435 440 445
Ala Leu Thr Cys Leu Val Ala Leu Thr Leu Thr Ser Thr Leu Val Ile
450 455 460
Pro Leu His Leu Leu Ser Lys Gln Leu Leu Gln Ala Leu Val Val Lys
465 470 475 480
Arg Leu Cys Leu Gln Lys Thr Leu Lys Gln Ile Val Val Leu Arg Asn
485 490 495
Leu Leu Leu Val Val His Pro Cys Gly Met Ala Lys Ser Lys Leu Met
500 505 510
Ala Met Gly Ser Arg Ala Leu Gln Met Tyr Leu Thr Thr Gln Leu Val
515 520 525
Leu Tyr Val Ser Leu Val Thr Leu Ser Ser Ser Ala Thr Gln Leu Leu
530 535 540
Val Ile Phe Glu Val Ile Leu Thr Ser Ser Phe Lys
545 550 555
<210> 115
<211> 169
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 115
Thr Cys Phe Ala Ile Phe His His Asn Val Ser Phe Val Lys Phe Tyr
1 5 10 15
Gly Ile Cys His Gln Gln Phe Ile Ser Val Asp Val Ala Arg Cys Val
20 25 30
Asn Tyr Cys Thr Arg Tyr Arg Leu Asn Ile Ser Ser Asn Leu Cys Gln
35 40 45
Val Val Asp Lys Pro Ser Ser Asn Val Arg Phe Pro Lys Leu Tyr Gln
50 55 60
Ile Leu Val Asp Ala Lys Asp Ile Thr Val Arg Asp Arg Gln Phe Thr
65 70 75 80
Cys Val Phe Asp Gln Leu Thr Gln Ile Phe His Thr Lys Cys Ala Arg
85 90 95
Val Tyr His Lys Thr Tyr Ala Arg Val Ser Val Leu Val His Ser Thr
100 105 110
Ile Gly Lys Cys Lys Ser Asp Phe Cys Ser Lys Cys Ala Asn Thr Thr
115 120 125
Phe Leu Asn His Lys Cys Phe Val Arg Phe Cys Ile Thr Thr Asn Leu
130 135 140
Phe Arg Ser Lys Thr Thr Tyr Cys Gly His Pro Ile Ala Phe Leu Gln
145 150 155 160
Phe Ser Tyr Ile Ala Ile Pro Glu Leu
165
<210> 116
<211> 792
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 116
Glu Glu Ser Ile Met Arg Met Val Asn Asp His Ala Glu Val Ile Lys
1 5 10 15
Ser Ser Thr Ser Leu Glu Thr Ser Gln Ala Gln Ile Thr Met Thr Pro
20 25 30
Glu Met Phe Ser Leu Leu Ser Ser Gly Val Tyr Thr Phe Lys Glu Arg
35 40 45
Ala Val Ile Arg Glu Leu Ser Cys Asn Ala Val Asp Ala Gln Lys Glu
50 55 60
Ala Gly Arg Glu Asn Ile Pro Phe His Val His Leu Pro Thr Arg Phe
65 70 75 80
Glu Pro Tyr Phe Glu Val Arg Asp Phe Gly Thr Gly Leu Thr His Asp
85 90 95
Lys Val Met Ser Leu Tyr Leu Asn Tyr Gly Ala Ser Thr Lys Asn Asp
100 105 110
Ser Asn Asp Tyr Ile Gly Ala Met Gly Ile Gly Ser Lys Ser Pro Phe
115 120 125
Ala Ile Ala Gln Ser Phe Thr Val Ser Ser Tyr Val Asp Gly Val Val
130 135 140
Asn Lys Tyr Ser Val Tyr Leu Glu Asn Gly Ile Pro Gln Val Thr Lys
145 150 155 160
Leu Thr Thr Asn Pro Thr Lys Glu Pro Asn Gly Leu Ala Val Arg Val
165 170 175
Ala Val Ala Asp His Arg Ile Ser Lys Phe Phe Glu Glu Ala Gly Asn
180 185 190
Val Tyr Ser Tyr Phe Ala Val Lys Pro Glu Ser Asn Ile Val Tyr Asp
195 200 205
Asp Val Leu Ala Asp Met Asn Val Ile Ala Arg Glu Glu Gly Val Tyr
210 215 220
Asp Ala Met Ile His Lys Gln Ser Trp Arg Ser Ser Gly Asn Arg Thr
225 230 235 240
Glu Phe Asn Val Val Met Gly Asn Ile Ala Tyr Pro Val Asn Met Glu
245 250 255
Ala Leu Leu Gly Asp Asp Phe Phe Lys Val Leu Pro Glu Phe Phe Arg
260 265 270
Arg Ser Val Asp Leu Val Asn Ile Tyr Met Pro Ile Gly Ser Val Ala
275 280 285
Ile Ala Ala Ser Arg Glu Ala Leu Gln Met Asn Asp Thr Thr Lys Asn
290 295 300
Val Ile Ile Glu Ala Thr Lys Lys Ile Thr Glu Ala Ile Thr Lys Asp
305 310 315 320
Val Ile Lys Lys Val Asn Ser Gln Pro Thr Leu Met Asp Ala Ala Gln
325 330 335
Ala Tyr Ala Glu Leu Arg Leu Asn Ser Arg Glu Met Phe Asn Ala Val
340 345 350
Cys Pro Lys Leu Glu Trp Gly Gly Val Lys Leu Asp Ser Leu Glu Glu
355 360 365
Glu Leu Leu Asn Ile Arg Arg Gly Ile Ile Tyr Ala Glu Asp Gly Ser
370 375 380
Val Ile Tyr Glu Arg Asp Gly Lys Gly Asn Ile Lys Val Asp Ser Lys
385 390 395 400
Gly Asn Asn Ile Pro Lys Val Asp Tyr Leu Tyr Asn Pro Val Ala Tyr
405 410 415
Val Lys Phe Asn Ser Leu Glu Ser Lys Ile Arg Ala Thr Ala Leu Ser
420 425 430
Tyr Thr Gln Glu Ala Ser Met Phe Asn Ile Phe Gly Ala Met Arg Lys
435 440 445
Ser Gln Ile Glu Gln Phe Leu Phe Val Ile Asn Asp Arg Arg Asn Lys
450 455 460
Asn Gly Thr Glu Lys Thr Val Gly Arg Asn Gln Ile Leu Arg Gly Ala
465 470 475 480
Cys Arg Asp Tyr Ala Ser Glu Ser Asn Leu Phe His Arg Tyr Asn Gly
485 490 495
Ile Val Phe Val Phe Ser Thr Glu Lys Glu Leu Asp Asp Leu Ile Asn
500 505 510
Leu His Lys Leu Asp Lys Ser Leu Leu Lys Ile Val Lys Met Ser Asp
515 520 525
Lys Glu His His Tyr Gln Arg Lys Glu Ala Val Arg Gly Val Val Lys
530 535 540
Leu Trp Lys Ala Val Pro Ala Glu Thr Lys Ala Ser Tyr Lys Glu Val
545 550 555 560
Ser Glu Asp Leu Asp Thr Ile Glu Glu Pro Gln Leu Tyr Ile Lys Ala
565 570 575
Val Gly Asp Thr Val Asp Gly Arg Cys Phe Cys Ser Ser Pro Glu Asp
580 585 590
Val Ala Lys Ser Val Ala Asn Val Thr Gly Lys Thr Val Tyr Val Phe
595 600 605
Arg Lys Ala Asn Trp Lys Lys Ile Pro Glu Asp Trp Ile Glu Val Asp
610 615 620
Glu Lys Leu Leu Asn Asp Ser Leu Thr Asp Val His Trp Ile Asn His
625 630 635 640
Asn Arg Tyr Met Thr Arg Ile Tyr Met Asp Gly Val Leu Asp Leu Thr
645 650 655
Ser Ser Trp Ile Ile Ala Arg Asn Phe Thr Phe Asn Asn Arg Lys Ile
660 665 670
Ser Arg Gly Tyr Cys Tyr Ser Arg Asp Thr Ile Lys Thr Ile Phe Leu
675 680 685
Glu Gly Asn Glu Asp Ala Val Glu Ala Met Phe Gly Lys Ile Gln Tyr
690 695 700
Val Ala Ala Pro Phe Ala Tyr Thr Tyr Thr Ile Ser Val Leu Gln Asp
705 710 715 720
Leu Lys Ser Cys Leu Asp Asn Asp Thr Lys Leu Tyr Lys Lys Ile Lys
725 730 735
Lys Ala Gly Asp Arg Met Val Ile Lys Leu Thr Asn Tyr Leu Ser Lys
740 745 750
Arg Lys Gln Glu Asn Phe Leu Leu Ser His Leu Asp Trp Asn Lys Val
755 760 765
Ser Pro Ile Glu Val Ser Lys Phe Leu Gly Phe Asn Val Lys Cys Val
770 775 780
Pro Glu Gly Thr Thr Ile Tyr Asp
785 790
<210> 117
<211> 788
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 117
Met Arg Met Val Asn Asp His Ala Glu Val Ile Lys Ser Ser Thr Ser
1 5 10 15
Leu Glu Thr Ser Gln Ala Gln Ile Thr Met Thr Pro Glu Met Phe Ser
20 25 30
Leu Leu Ser Ser Gly Val Tyr Thr Phe Lys Glu Arg Ala Val Ile Arg
35 40 45
Glu Leu Ser Cys Asn Ala Val Asp Ala Gln Lys Glu Ala Gly Arg Glu
50 55 60
Asn Ile Pro Phe His Val His Leu Pro Thr Arg Phe Glu Pro Tyr Phe
65 70 75 80
Glu Val Arg Asp Phe Gly Thr Gly Leu Thr His Asp Lys Val Met Ser
85 90 95
Leu Tyr Leu Asn Tyr Gly Ala Ser Thr Lys Asn Asp Ser Asn Asp Tyr
100 105 110
Ile Gly Ala Met Gly Ile Gly Ser Lys Ser Pro Phe Ala Ile Ala Gln
115 120 125
Ser Phe Thr Val Ser Ser Tyr Val Asp Gly Val Val Asn Lys Tyr Ser
130 135 140
Val Tyr Leu Glu Asn Gly Ile Pro Gln Val Thr Lys Leu Thr Thr Asn
145 150 155 160
Pro Thr Lys Glu Pro Asn Gly Leu Ala Val Arg Val Ala Val Ala Asp
165 170 175
His Arg Ile Ser Lys Phe Phe Glu Glu Ala Gly Asn Val Tyr Ser Tyr
180 185 190
Phe Ala Val Lys Pro Glu Ser Asn Ile Val Tyr Asp Asp Val Leu Ala
195 200 205
Asp Met Asn Val Ile Ala Arg Glu Glu Gly Val Tyr Asp Ala Met Ile
210 215 220
His Lys Gln Ser Trp Arg Ser Ser Gly Asn Arg Thr Glu Phe Asn Val
225 230 235 240
Val Met Gly Asn Ile Ala Tyr Pro Val Asn Met Glu Ala Leu Leu Gly
245 250 255
Asp Asp Phe Phe Lys Val Leu Pro Glu Phe Phe Arg Arg Ser Val Asp
260 265 270
Leu Val Asn Ile Tyr Met Pro Ile Gly Ser Val Ala Ile Ala Ala Ser
275 280 285
Arg Glu Ala Leu Gln Met Asn Asp Thr Thr Lys Asn Val Ile Ile Glu
290 295 300
Ala Thr Lys Lys Ile Thr Glu Ala Ile Thr Lys Asp Val Ile Lys Lys
305 310 315 320
Val Asn Ser Gln Pro Thr Leu Met Asp Ala Ala Gln Ala Tyr Ala Glu
325 330 335
Leu Arg Leu Asn Ser Arg Glu Met Phe Asn Ala Val Cys Pro Lys Leu
340 345 350
Glu Trp Gly Gly Val Lys Leu Asp Ser Leu Glu Glu Glu Leu Leu Asn
355 360 365
Ile Arg Arg Gly Ile Ile Tyr Ala Glu Asp Gly Ser Val Ile Tyr Glu
370 375 380
Arg Asp Gly Lys Gly Asn Ile Lys Val Asp Ser Lys Gly Asn Asn Ile
385 390 395 400
Pro Lys Val Asp Tyr Leu Tyr Asn Pro Val Ala Tyr Val Lys Phe Asn
405 410 415
Ser Leu Glu Ser Lys Ile Arg Ala Thr Ala Leu Ser Tyr Thr Gln Glu
420 425 430
Ala Ser Met Phe Asn Ile Phe Gly Ala Met Arg Lys Ser Gln Ile Glu
435 440 445
Gln Phe Leu Phe Val Ile Asn Asp Arg Arg Asn Lys Asn Gly Thr Glu
450 455 460
Lys Thr Val Gly Arg Asn Gln Ile Leu Arg Gly Ala Cys Arg Asp Tyr
465 470 475 480
Ala Ser Glu Ser Asn Leu Phe His Arg Tyr Asn Gly Ile Val Phe Val
485 490 495
Phe Ser Thr Glu Lys Glu Leu Asp Asp Leu Ile Asn Leu His Lys Leu
500 505 510
Asp Lys Ser Leu Leu Lys Ile Val Lys Met Ser Asp Lys Glu His His
515 520 525
Tyr Gln Arg Lys Glu Ala Val Arg Gly Val Val Lys Leu Trp Lys Ala
530 535 540
Val Pro Ala Glu Thr Lys Ala Ser Tyr Lys Glu Val Ser Glu Asp Leu
545 550 555 560
Asp Thr Ile Glu Glu Pro Gln Leu Tyr Ile Lys Ala Val Gly Asp Thr
565 570 575
Val Asp Gly Arg Cys Phe Cys Ser Ser Pro Glu Asp Val Ala Lys Ser
580 585 590
Val Ala Asn Val Thr Gly Lys Thr Val Tyr Val Phe Arg Lys Ala Asn
595 600 605
Trp Lys Lys Ile Pro Glu Asp Trp Ile Glu Val Asp Glu Lys Leu Leu
610 615 620
Asn Asp Ser Leu Thr Asp Val His Trp Ile Asn His Asn Arg Tyr Met
625 630 635 640
Thr Arg Ile Tyr Met Asp Gly Val Leu Asp Leu Thr Ser Ser Trp Ile
645 650 655
Ile Ala Arg Asn Phe Thr Phe Asn Asn Arg Lys Ile Ser Arg Gly Tyr
660 665 670
Cys Tyr Ser Arg Asp Thr Ile Lys Thr Ile Phe Leu Glu Gly Asn Glu
675 680 685
Asp Ala Val Glu Ala Met Phe Gly Lys Ile Gln Tyr Val Ala Ala Pro
690 695 700
Phe Ala Tyr Thr Tyr Thr Ile Ser Val Leu Gln Asp Leu Lys Ser Cys
705 710 715 720
Leu Asp Asn Asp Thr Lys Leu Tyr Lys Lys Ile Lys Lys Ala Gly Asp
725 730 735
Arg Met Val Ile Lys Leu Thr Asn Tyr Leu Ser Lys Arg Lys Gln Glu
740 745 750
Asn Phe Leu Leu Ser His Leu Asp Trp Asn Lys Val Ser Pro Ile Glu
755 760 765
Val Ser Lys Phe Leu Gly Phe Asn Val Lys Cys Val Pro Glu Gly Thr
770 775 780
Thr Ile Tyr Asp
785
<210> 118
<211> 788
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 118
Met Arg Met Val Asn Asp His Ala Glu Val Ile Lys Ser Ser Thr Ser
1 5 10 15
Leu Glu Thr Ser Gln Ala Gln Ile Thr Met Thr Pro Glu Met Phe Ser
20 25 30
Leu Leu Ser Ser Gly Val Tyr Thr Phe Lys Glu Arg Ala Val Ile Arg
35 40 45
Glu Leu Ser Cys Asn Ala Val Asp Ala Gln Lys Glu Ala Gly Arg Glu
50 55 60
Asn Ile Pro Phe His Val His Leu Pro Thr Arg Phe Glu Pro Tyr Phe
65 70 75 80
Glu Val Arg Asp Phe Gly Thr Gly Leu Thr His Asp Lys Val Met Ser
85 90 95
Leu Tyr Leu Asn Tyr Gly Ala Ser Thr Lys Asn Asp Ser Asn Asp Tyr
100 105 110
Ile Gly Ala Met Gly Ile Gly Ser Lys Ser Pro Phe Ala Ile Ala Gln
115 120 125
Ser Phe Thr Val Ser Ser Tyr Val Asp Gly Val Val Asn Lys Tyr Ser
130 135 140
Val Tyr Leu Glu Asn Gly Ile Pro Gln Val Thr Lys Leu Thr Thr Asn
145 150 155 160
Pro Thr Lys Glu Pro Asn Gly Leu Ala Val Arg Val Ala Val Ala Asp
165 170 175
His Arg Ile Ser Lys Phe Phe Glu Glu Ala Gly Asn Val Tyr Ser Tyr
180 185 190
Phe Ala Val Lys Pro Glu Ser Asn Ile Val Tyr Asp Asp Val Leu Ala
195 200 205
Asp Met Asn Val Ile Ala Arg Glu Glu Gly Val Tyr Asp Ala Met Ile
210 215 220
His Lys Gln Ser Trp Arg Ser Ser Gly Asn Arg Thr Glu Phe Asn Val
225 230 235 240
Val Met Gly Asn Ile Ala Tyr Pro Val Asn Met Glu Ala Leu Leu Gly
245 250 255
Asp Asp Phe Phe Lys Val Leu Pro Glu Phe Phe Arg Arg Ser Val Asp
260 265 270
Leu Val Asn Ile Tyr Met Pro Ile Gly Ser Val Ala Ile Ala Ala Ser
275 280 285
Arg Glu Ala Leu Gln Met Asn Asp Thr Thr Lys Asn Val Ile Ile Glu
290 295 300
Ala Thr Lys Lys Ile Thr Glu Ala Ile Thr Lys Asp Val Ile Lys Lys
305 310 315 320
Val Asn Ser Gln Pro Thr Leu Met Asp Ala Ala Gln Ala Tyr Ala Glu
325 330 335
Leu Arg Leu Asn Ser Arg Glu Met Phe Asn Ala Val Cys Pro Lys Leu
340 345 350
Glu Trp Gly Gly Val Lys Leu Asp Ser Leu Glu Glu Glu Leu Leu Asn
355 360 365
Ile Arg Arg Gly Ile Ile Tyr Ala Glu Asp Gly Ser Val Ile Tyr Glu
370 375 380
Arg Asp Gly Lys Gly Asn Ile Lys Val Asp Ser Lys Gly Asn Asn Ile
385 390 395 400
Pro Lys Val Asp Tyr Leu Tyr Asn Pro Val Ala Tyr Val Lys Phe Asn
405 410 415
Ser Leu Glu Ser Lys Ile Arg Ala Thr Ala Leu Ser Tyr Thr Gln Glu
420 425 430
Ala Ser Met Phe Asn Ile Phe Gly Ala Met Arg Lys Ser Gln Ile Glu
435 440 445
Gln Phe Leu Phe Val Ile Asn Asp Arg Arg Asn Lys Asn Gly Thr Glu
450 455 460
Lys Thr Val Gly Arg Asn Gln Ile Leu Arg Gly Ala Cys Arg Asp Tyr
465 470 475 480
Ala Ser Glu Ser Asn Leu Phe His Arg Tyr Asn Gly Ile Val Phe Val
485 490 495
Phe Ser Thr Glu Lys Glu Leu Asp Asp Leu Ile Asn Leu His Lys Leu
500 505 510
Asp Lys Ser Leu Leu Lys Ile Val Lys Met Ser Asp Lys Glu His His
515 520 525
Tyr Gln Arg Lys Glu Ala Val Arg Gly Val Val Lys Leu Trp Lys Ala
530 535 540
Val Pro Ala Glu Thr Lys Ala Ser Tyr Lys Glu Val Ser Glu Asp Leu
545 550 555 560
Asp Thr Ile Glu Glu Pro Gln Leu Tyr Ile Lys Ala Val Gly Asp Thr
565 570 575
Val Asp Gly Arg Cys Phe Cys Ser Ser Pro Glu Asp Val Ala Lys Ser
580 585 590
Val Ala Asn Val Thr Gly Lys Thr Val Tyr Val Phe Arg Lys Ala Asn
595 600 605
Trp Lys Lys Ile Pro Glu Asp Trp Ile Glu Val Asp Glu Lys Leu Leu
610 615 620
Asn Asp Ser Leu Thr Asp Val His Trp Ile Asn His Asn Arg Tyr Met
625 630 635 640
Thr Arg Ile Tyr Met Asp Gly Val Leu Asp Leu Thr Ser Ser Trp Ile
645 650 655
Ile Ala Arg Asn Phe Thr Phe Asn Asn Arg Lys Ile Ser Arg Gly Tyr
660 665 670
Cys Tyr Ser Arg Asp Thr Ile Lys Thr Ile Phe Leu Glu Gly Asn Glu
675 680 685
Asp Ala Val Glu Ala Met Phe Gly Lys Ile Gln Tyr Val Ala Ala Pro
690 695 700
Phe Ala Tyr Thr Tyr Thr Ile Ser Val Leu Gln Asp Leu Lys Ser Cys
705 710 715 720
Leu Asp Asn Asp Thr Lys Leu Tyr Lys Lys Ile Lys Lys Ala Gly Asp
725 730 735
Arg Met Val Ile Lys Leu Thr Asn Tyr Leu Ser Lys Arg Lys Gln Glu
740 745 750
Asn Phe Leu Leu Ser His Leu Asp Trp Asn Lys Val Ser Pro Ile Glu
755 760 765
Val Ser Lys Phe Leu Gly Phe Asn Val Lys Cys Val Pro Glu Gly Thr
770 775 780
Thr Ile Tyr Asp
785
<210> 119
<211> 376
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 119
Leu Thr Arg Glu Ser Asn Lys Met Thr Thr Lys Thr Lys Ala Gln Ile
1 5 10 15
Asp Ala Glu Ile Tyr Lys Leu Val Lys Glu Gly Lys Leu Thr Lys Thr
20 25 30
Ala Ile Ala Gln Lys Phe Asn Thr Ser Thr Arg Ser Val Gly Arg Ala
35 40 45
Val Glu Arg His Glu Ala Thr Leu Lys Gly Lys Lys Lys Ala Thr Ser
50 55 60
Thr Pro Ala Thr Lys Thr Leu Lys Gln Val Ala Lys Thr Phe Lys Lys
65 70 75 80
Lys Ala Gly Lys Pro Val Glu Lys Val Val Lys Asp Thr Val Gln Lys
85 90 95
Ala Pro Val Asn Lys Leu His Glu Ala Met Gln Lys Asp Asp Lys Ile
100 105 110
Glu Tyr Met Ile Thr Gly Asp Ser Val Ile Met Thr Tyr Gly Ser Glu
115 120 125
Ser Glu Ile Val Glu Ser Thr His Pro Asn Tyr Gln Glu Ile Val Val
130 135 140
His Val Val Lys Gly Glu Phe Lys Lys Ala Phe Glu Leu Met Asn Ile
145 150 155 160
Arg Lys Ser Ile Glu Asn Phe Thr Gln Gly Ala Ile Thr Ile Lys Gly
165 170 175
Asp Lys Leu Phe Tyr Gly Ala Val Glu Met Arg Ser Thr Leu Val Asp
180 185 190
Arg Ile Leu His Met Met Lys Thr Gly Asp Lys Gly Phe Glu Arg Leu
195 200 205
Val Met Phe Phe Glu Lys Leu Met Glu Asn Pro Ser Lys Asp Ser Val
210 215 220
Glu Gln Leu Trp Gly Phe Val Ser His Leu Asp Val Glu Ile Asp Glu
225 230 235 240
Glu Gly Tyr Ile Ile Gly Trp Lys Lys Val Ser Thr Arg Glu Gly Lys
245 250 255
Leu Val Asp Ser His Thr Tyr Lys Val Pro Asn Asp Leu Gly Asn Ile
260 265 270
Val Glu Met Pro Arg Trp Met Val Asp Asn Asn Arg Asn Val Thr Cys
275 280 285
Ser Gln Gly Leu His Val Gly Ala Trp Asp Tyr Val Arg Cys Phe Ser
290 295 300
Gly Asp Thr Ile Leu Lys Val Arg Val His Pro Arg Asp Val Val Ser
305 310 315 320
Val Pro Thr Asp Tyr Asn Asp Met Lys Met Arg Ser Ser Arg Tyr Glu
325 330 335
Val Ala Ala Ile Val Asp Asn Gln Arg Lys Val Leu Glu Ala Trp Asp
340 345 350
Gly Lys Thr Glu Ala Leu His Val Ile Val Gly Thr Ala Gly Glu Leu
355 360 365
Ile Ser Gln Arg Lys Arg Glu Ile
370 375
<210> 120
<211> 369
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 120
Met Thr Thr Lys Thr Lys Ala Gln Ile Asp Ala Glu Ile Tyr Lys Leu
1 5 10 15
Val Lys Glu Gly Lys Leu Thr Lys Thr Ala Ile Ala Gln Lys Phe Asn
20 25 30
Thr Ser Thr Arg Ser Val Gly Arg Ala Val Glu Arg His Glu Ala Thr
35 40 45
Leu Lys Gly Lys Lys Lys Ala Thr Ser Thr Pro Ala Thr Lys Thr Leu
50 55 60
Lys Gln Val Ala Lys Thr Phe Lys Lys Lys Ala Gly Lys Pro Val Glu
65 70 75 80
Lys Val Val Lys Asp Thr Val Gln Lys Ala Pro Val Asn Lys Leu His
85 90 95
Glu Ala Met Gln Lys Asp Asp Lys Ile Glu Tyr Met Ile Thr Gly Asp
100 105 110
Ser Val Ile Met Thr Tyr Gly Ser Glu Ser Glu Ile Val Glu Ser Thr
115 120 125
His Pro Asn Tyr Gln Glu Ile Val Val His Val Val Lys Gly Glu Phe
130 135 140
Lys Lys Ala Phe Glu Leu Met Asn Ile Arg Lys Ser Ile Glu Asn Phe
145 150 155 160
Thr Gln Gly Ala Ile Thr Ile Lys Gly Asp Lys Leu Phe Tyr Gly Ala
165 170 175
Val Glu Met Arg Ser Thr Leu Val Asp Arg Ile Leu His Met Met Lys
180 185 190
Thr Gly Asp Lys Gly Phe Glu Arg Leu Val Met Phe Phe Glu Lys Leu
195 200 205
Met Glu Asn Pro Ser Lys Asp Ser Val Glu Gln Leu Trp Gly Phe Val
210 215 220
Ser His Leu Asp Val Glu Ile Asp Glu Glu Gly Tyr Ile Ile Gly Trp
225 230 235 240
Lys Lys Val Ser Thr Arg Glu Gly Lys Leu Val Asp Ser His Thr Tyr
245 250 255
Lys Val Pro Asn Asp Leu Gly Asn Ile Val Glu Met Pro Arg Trp Met
260 265 270
Val Asp Asn Asn Arg Asn Val Thr Cys Ser Gln Gly Leu His Val Gly
275 280 285
Ala Trp Asp Tyr Val Arg Cys Phe Ser Gly Asp Thr Ile Leu Lys Val
290 295 300
Arg Val His Pro Arg Asp Val Val Ser Val Pro Thr Asp Tyr Asn Asp
305 310 315 320
Met Lys Met Arg Ser Ser Arg Tyr Glu Val Ala Ala Ile Val Asp Asn
325 330 335
Gln Arg Lys Val Leu Glu Ala Trp Asp Gly Lys Thr Glu Ala Leu His
340 345 350
Val Ile Val Gly Thr Ala Gly Glu Leu Ile Ser Gln Arg Lys Arg Glu
355 360 365
Ile
<210> 121
<211> 113
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 121
Glu Val Thr Ser Asn Leu His Thr Arg Ser Gln Asn Val Thr Asn Lys
1 5 10 15
Val His His Phe Ser Ala Ile Phe Thr Lys Phe Ser Lys Tyr His Glu
20 25 30
Glu Phe Phe Gln His Phe Val Trp Arg Phe Ile Arg Tyr Phe Leu Lys
35 40 45
Pro Ser Lys Tyr Arg Val Lys Ile Asn Phe Asn Ile Phe Phe Ala Val
50 55 60
Asp Asn Thr Pro Leu Phe Asp Arg Ser Thr Phe Ala Leu Gln Asn Val
65 70 75 80
Arg Val Gly Val Phe His Arg Ile Ile Gln Thr Val Asn Tyr Phe His
85 90 95
Trp Thr Pro His Asn Ile Ile Val Thr Val Tyr Ser Ala Arg Leu Thr
100 105 110
Lys
<210> 122
<211> 65
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 122
Val Asn Ser Thr Phe Lys Asn Ser Gln Val Ser Glu Ser Asp Lys Tyr
1 5 10 15
Thr Gln Glu Glu Ser Arg Tyr Ile Ile Lys Gly Asn Thr Gly Ser Asp
20 25 30
Val Ala Ala Ala Leu Glu Phe Tyr Arg Asp Gly Phe Tyr Gln Cys Thr
35 40 45
Ile Lys Ala Asn Asn Leu Ile Asp Met Ile Leu Leu Gly Asn Lys Gln
50 55 60
Gln
65
<210> 123
<211> 108
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 123
Met Gln Ile Asn Gly Arg Asp Phe Val Ala Val Tyr Tyr Glu Lys Gly
1 5 10 15
Lys Glu Val Gly Val Ala Gln Val Ser Tyr Gly Asn Gly Lys Trp Leu
20 25 30
Tyr Gly Thr Ile Ala Val Val Gly Thr Arg Ser Asp Thr Lys Thr Phe
35 40 45
Lys Asp Cys Val Asp Leu Leu Glu Glu Ser Ile Gln Asn His Trp Cys
50 55 60
Leu Ile Trp Met Thr Asp Asn Glu Val Ile Glu Arg Phe Lys Lys Ile
65 70 75 80
Asp Ile Asn Ile Asp Ser Ile Glu His Val Asp Leu Tyr Glu Leu Thr
85 90 95
Glu Lys Val Asn Tyr Glu Ser Asn Ser Ser Gln Arg
100 105
<210> 124
<211> 261
<212> PRT
<213> 未知
<220>
<223> 在分离自环境的噬菌体中发现
<400> 124
Met Gly Ile Val Lys Ile Ile Lys Gly Asp Ile Phe Asp Ala Phe Asp
1 5 10 15
Lys Gly Lys Phe Asp Ile Ile Gly His Gly Cys Asn Cys Met Asn Phe
20 25 30
Met Gly Ala Gly Ile Ala Ala Thr Ile Ser Lys Arg Tyr Pro Lys Val
35 40 45
Tyr Asp Thr Asp Thr Glu Val Tyr Leu Phe Ala Gly Gly Ile Arg His
50 55 60
Lys Pro Cys Glu Asn Leu Leu Gly Asn Phe Ser Val Ala Arg Leu Lys
65 70 75 80
Gln Gly Arg Ile Ala Asn Leu Tyr Thr Gln Leu His Thr Gly Lys Asp
85 90 95
Ala Arg Tyr Ser Ala Leu Glu Ser Ser Leu Lys Gln Leu Asn Arg Tyr
100 105 110
Cys Glu Val Asn Gln Leu Lys Lys Val Gly Leu Pro Met Ile Gly Ala
115 120 125
Gly Ile Gly Gly Leu Asp Pro Gln Ala Val Thr Val Ile Ile Asn Gln
130 135 140
Val Met Lys Ser Val Asp Val Tyr Leu Tyr Val Tyr Glu Ala Lys Met
145 150 155 160
Tyr Asn Glu Leu His Ser Gly Trp Lys Asn Tyr Cys Glu Pro Glu Tyr
165 170 175
Phe Ala Gly Val Val Val Phe Thr Asp Asn Glu Val Thr Leu Phe Arg
180 185 190
Arg Arg Lys Gly Lys Ile His Gln Ser Asn Pro Pro Val Glu Lys Met
195 200 205
Ser Leu Ser Asn Ala Leu Val Thr His Leu Ser Lys Ser Asn His Arg
210 215 220
Ile Ala Val Thr Phe Gly Ser Asp Ala Asp Thr Tyr Ile Tyr Ala Arg
225 230 235 240
Thr Asp Glu Asp Ile Glu Thr Val Phe Ser Ser Pro Glu Val Thr Phe
245 250 255
Leu Asp Ala Lys Asn
260
<210> 125
<211> 876
<212> DNA
<213> 肠沙门氏菌
<400> 125
atgtcgcgac tggcgcagga tatgaaaaaa ctggcgcacc gcgcaggtgg cagccacaaa 60
accgtccacg accgcgaaca gatggcgcag cgttttgccc gtcatttgct ggcgcagaat 120
attcaggtca ccagcacctg ccagctcaaa gccagacaca tcgcgggata tattcatgaa 180
tggctggcgc agggtatttc tctgcgaacg ctacagaatg agatggcaat ggtgcgctct 240
attctggctg aagccggacg cacacaactc agccagtcag aattaatcag caaccagtcg 300
ctcggtatca gtggtgccag ctgtgacggg acgcaccggg ccattccgga tgcactttac 360
catcaggtac tggaccgggt tcatcatatc gacgcaggcc tcgcagccag cctgcaactg 420
gcgcgggtga tggggctgcg cggtcaggaa gcagtacagt gctgccagtc gctgaaaacc 480
tgggataaac agctggaaaa aggggctgaa cgtctgccgg tgatcttcgg caccaaaggc 540
gggcgaccac gcatgacaca ggtgacggac cgggaagcgg ttcgtcaggc agtgaaagag 600
gcgcttggga ttgccgggga gcgtaacggg catctcattg acaagccgga tctgaaatct 660
gcaatggact actggcacaa ccatctccgc gataccgggc tgacggggga atactctccc 720
cactccctgc gctatgcctg ggcgcaggat gcgatccgct attacgaaga gcagggtctg 780
agccataaag aggcgctggc ggtaacttca acggatctgg gccatggtga tgggcgggga 840
atatatattg agcaggtata cgggtatact gattga 876
<210> 126
<211> 876
<212> DNA
<213> 肺炎克雷伯菌
<400> 126
atgtcacgtc tggcgcagga tatgaaaaaa ctggcgcacc gtgcgggtgg tagccataaa 60
acggtccatg atcgcgaaca gatggcgcag cgtttcacac atcatttgct ggcgcagaat 120
atccagataa cccgaaccga ccatattaag gccaggcaca tcacaggcta tatcaatgaa 180
agactggcgc agggcatttc cccgcgtacc ttacagaatg agatggcgat ggtgcgctct 240
attctgactg aagccgggcg tacacaactc agccagtcag aattaatcag caaccaatcg 300
ctcggtatca acggtgcctg ccgcgatgga acacatcgag caattccgga tgcgttttac 360
cagcaggtgc tggagcggat acagcaaacc gacgctggtc tcgcggccag cctgcaactg 420
gcaagggtga tgggcctgcg tggtcaggag gcggtacagt gctgccagtc actgaaaacc 480
tgggataaac agctggaaaa aggggctgaa cgtctgcccg tgattttcgg caccaagggc 540
ggacgaccac gcatgacgca ggtgacggac cgggaagcgg ttcgtcaggc ggtgaaagag 600
gcactgaata ttgccgggga gcgtaacgga catctgattg acaagccgga tctgaaatcc 660
gcgatggatt actggcacaa ccacctccgc gatgctggac tgacgggcga atactcgccc 720
cactccctgc gctatgcctg ggcacaggat gcgatccggt attacgagga gcaggggctg 780
agtcacaaag aggcgctggc ggtaacctca acggatctgg ggcacggtga cgggcgcggg 840
cggtatatcg agcagattta cgggcagact ggctga 876
<210> 127
<211> 366
<212> DNA
<213> 蜡状芽孢杆菌
<400> 127
atggaggaaa gagatatgaa taaatcaaca gttcaaatag ctttaagtat tacaaaattt 60
tcagcacaga agggatggaa tgatgaagaa ttttgggaag caatcgaatt acttcgatta 120
aataaggaag atacaagaca aacaatgata gaaaaattag atgggattcc agttacttac 180
gataatacaa atgattatcc gattatgttg aaagtagatc atgtggcaga agtattaggg 240
atttcgtcaa gaaaagcata tgacattatg gatcaaaaag gattcccatt agtaaaaatc 300
gggcgtagga aagtggtacc aagggatgca ttctttaatt ggctcgaaaa ggaggtgtca 360
gcatag 366
<210> 128
<211> 759
<212> DNA
<213> 阴沟肠杆菌
<400> 128
atgccgatta ttcagtctgt tgaacgtgcg ttgcagatcc tcgacctgtt caacgagcag 60
gccaccgagc ttaagatcac cgacatcagc aaactgatgg ggctaagcaa gagtaccctc 120
cactcgttgc tgaaaaccct gcagcttcat ggctatatcg atcagaaccc ggagaacggc 180
aagtatcgcc tcggcatgaa gctggtcgag cgcggtcatt tcgttgtggg ctccatcgat 240
attcggcaga aggcgaaagg ctggctgacg gagctgtcac agcggaccgg gcagacgacc 300
catctgggga tcctggacgg gcgtgaaggg gtttatatcg agaagattga aggcaagctg 360
gccgccatcg cctattcgcg cattggccgc cgtctgccgg ttcacgccac cgccatcggc 420
aaggtgttga ttgcctggct gggcgaaacc gagctgaacg ccctgcttga gggttatcaa 480
tacactacct acaccccttc caccctcgcc tctcgcgaag ccttaatggc cgccctggcg 540
cagacccgcg agcagggcta tgccctggat agcgaagaga acgagcaggg cgtgcgctgc 600
gtggcggtgc cggtgtggaa ccacgagtcc cgcgtcattg ccgccctgag cctgtcgacg 660
ctgacctccc gcgtggacga cgaagagctg gctaatttcc gcgagcagct tcagcaggcc 720
gggctccagc tctcacgcgc gctgggctac ccggcctga 759
<210> 129
<211> 246
<212> DNA
<213> 副溶血性弧菌
<400> 129
atgcctgtgt gtgctctacc taacgcggac ggttttctcg ctgtcgttcc tgacattgaa 60
gcggcttcat gccgtggtta tgtcatggtg acggctcaag aatatgacac gttaatgagc 120
tacacacagc tgactccagg agagatatct caagcgttcg gcttgggttt caccttggtg 180
ttcgttggcg gatatctctc aacttacgcc atcaagatgg caatacgttt aataaaacta 240
ctttga 246
<210> 130
<211> 348
<212> DNA
<213> 苏云金芽孢杆菌
<400> 130
atgaaaaatt ctacagttca aatcgcgctg gcaattacaa agttttcagc tcaaaaggga 60
tggaaggatg aagagttttg ggaggctatt gagttacttc aattcaataa agaagatgaa 120
aggcaaacaa ctgtagaaaa gctagatagc attccagtta ctaatgatag tacagattat 180
ccaattatgt taaacgtaac acacttagaa gaaatattag gagtagcgaa aaggacagct 240
tacgaaatta tggatcaaaa aggattcccg ttagtgaaaa tcgggcgtaa gaaggcggtt 300
ccaagggatg cattctttaa ttggttagaa aagggggtgt cagcatga 348
<210> 131
<211> 741
<212> DNA
<213> 粪肠球菌
<400> 131
atggtaaaaa aaataaaagc cgaagatcta tcagttgaaa aaggaactta catgatttac 60
gcaaatcctg gtatgggaaa aacttattct ttaggatttc ttccaggtaa atcattggta 120
ttagatgttg acggatcatc atcgacattg gcaaagcatc ctaacaaaga aaatattgaa 180
gtgtgggaat tagattcttc tgatatttgg caagaatggc ttgatactat ttcagactta 240
gtcgcaaata aatcttccta cgaaaagcaa ttcgacaata tttgtgtaga taacatttct 300
gaattgttca aagcgcaatt ggaagattta ggcaagaaag gaaaaaatag tggtgttcct 360
tctcaagccg actatcaaag aacagatttt atgaatttgc gaggtcttag agcattgaat 420
aatttagatt gccgaatagt cctgactgca tgggaaacaa cagacacata tacagaacct 480
aatggacaat tttttacaag atcaatgcca gatataagat caaaaatttt aaataatttt 540
ttaggcttat gtgatgtagt tggcagactt gtaataaaaa aagatgacga tggtaatgaa 600
acaagaggct taatactaaa gcctactagt aacgtttatg caaaaaacag attagatgaa 660
cgtagtgggt gtttggtgga acaattggtt gttagagcag gcggtgaacc gaatgtatca 720
actccgaccg tatcagatta a 741
<210> 132
<211> 261
<212> DNA
<213> 大肠杆菌O157
<400> 132
atgcgtaata tttctgtgtc tgcgccagct cctgctgcgc cattatttcc tgtatcgaac 60
catcatgagc gatttttacg cctgcctgag gtgatgcact tatgcgggtt gtcccgttcg 120
accgtttacg acctgatcag ccgcgatgcg tttccgaagc agatcccgct tggcggtaaa 180
aatgtcgcct gggcacagtc tgaggtcagc gcatggatgg cggaccgcat cagcgcccgt 240
gggcggggat gtgatgcatg a 261
<210> 133
<211> 894
<212> DNA
<213> 大肠杆菌str. K-12 substr. MC4100
<400> 133
atgaagctct tagcaaaagc acctctaaat ctgttacgcg cctttgaagc ggctggtcgt 60
accggagctt ttgcgttggc cgcctctgag ttggaactgt cacccagtgc gatcagccat 120
gccatccgca aactggaaaa cttgcttgat gtacgccttt ttcagcgcag tacgcgagaa 180
attacgctga cgaaagaagg tgaaatactg cttgagcaca tacagcgggg atttaacgaa 240
ttacagcagg ggttggcatt agtgacggct gatgaatcca ggcctttgcg ccttcatact 300
gcaccgagtt ttgcccatca atggctttta ccacgtcttg gtaagttcat acgtgagaac 360
ccaagcatcg atctgcgact ttcagccagc acagaatatg cacgttttga acaggatgat 420
tttgatctcg atatagtcta cggtgaaccc cgcccatcac cctatgagaa gatcccgctt 480
gctgttgaag aacttacgcc actgtgttct ccccaactgg ctgagcggct aaagaaacca 540
gaggatctct atgcgttgac attgattcag tgcgatgtgc agttgtacca gtggaaagga 600
tggtttgagg cgaataagat gacgccaccc aataattatg gccttcgatt tgaccgtagc 660
tttatggcga ttgctgccgc agttgacggc ttgggagttg tgcttgaatc aaaactactg 720
gctgaacgtg aaatcgctag cggtaaactg gtatgcccac ttgtaaacag taccagcgaa 780
atacattaca tcggtcatta tttggttttc ccccagcacc aacacatgca ttctgcgctt 840
gatgtgttca aaacctggct actgaatgaa ctcaatctgg gtaaaattcg ttga 894
<210> 134
<211> 477
<212> DNA
<213> 假伯克霍尔德氏菌
<400> 134
atgccgaagc tccatgaagc ggtcgccaac gggctgtccg aacgacttag aacgattcga 60
aaacgtgccg gcctctcgca agacgaactc gctgcgaggg cgtcgctggc tcgcacgaac 120
ctcgcgagcg tcgaacaagg acggcgggca aacctccgac tctcgaccct ttcccgtcta 180
gctgatgtac tgggcgtgga tgtgctcgac ttcttctgtg accggcctgt cggcgaacag 240
gaaccagccg gggaagacgc cccggctcgg gtaatagcga atgtgaaacg gctgcgcagt 300
gaggcagggc tcagtcaaga agcactctcg gtcaaagccc atcgatttcg gacgtacgtc 360
ggccggttgg agaacgaagc ggccagccca atggtggtcg atttgcagga cctggctgac 420
gcccttggca tctcgattcc ggcactcctc gagcctagcc agcacaaagc caaataa 477
<210> 135
<211> 897
<212> DNA
<213> 鼠疫耶尔森菌
<400> 135
atgttcgtaa tgagtcggtt aagtcgtgag atgaaaatgc tggcaaaaca agctggcggt 60
agccataaaa ccgttcatga tcgtattcgg attatggaca ggtttagccg ccatctgctg 120
gcccttaata ttcaggtgcg tgatgttaaa cacctaaaag caaagcatat tgaaagttat 180
attactgacc gtttttcaca ggggatcgcg atccgttctc ttcataacga aatggcggca 240
ttgcgcaccg ttttcagatt cgctggtcgg gataaaatcg tcttatcaga acgattaacc 300
aacaaagcat tggggttagg tggagccagc cgcgccggta gcaaattcgc tatccctgag 360
gaggtgtatc aagccgctct gcgcaccgcc caacagcaag acaaaggact cgcctgcgcc 420
cttcagcttg cccgcctgct ggggctgcgt tcacaagagg cggtgcaatg cgccaactcg 480
ttgaaaacat ggcaaaagga gcttgagcag aaccggtcta aactaaccgt ggtgtttggc 540
accaagggag gccgctcacg cgaaacccgc attctggatc ctgaagcagt caaacgggca 600
gtcaaagaag cgcaacagat cgccgaaaca cgcggcggca aacttattga taaacctgac 660
ctgaaaaccg cgatgaattt ctggcgctcc cataccaccc gcttagggct gaccggacat 720
tactcccccc acagcctgcg ctatgcctgg gcgcaagacg ctattaatta ttacctcgcc 780
gcaggattta gccgcattga agccagagcg ttaacgtcaa tggatctggg tcatggtgat 840
ggccgtggcc gctatgttga acgcgtttat acccgcaagg aggcgaacta tgtctga 897
<210> 136
<211> 321
<212> DNA
<213> 鼠疫耶尔森菌
<400> 136
atgattatgg ccaaagcccg tttacatgat gacgctatgg ttcagcttct tatggaagac 60
cctgaatttg cgcaagtata tctgcatcag gctcttctag atattgatga ggaaggaggg 120
caagaagcct ttctgatggc cctacgtcat gtagttgagg cccgaggagg gatggcaagt 180
gtggctaaaa aagcgggtgt atcgagagaa acactgtacc ggacgttatc accttcaggt 240
aatccaacac taaaaacact gcttagtgtg gtatctgcaa cgggatttca gttttcacat 300
cttgcatcga ttaccgcatg a 321
<210> 137
<211> 417
<212> DNA
<213> 胡萝卜欧文氏菌
<400> 137
atgtcccttg gcgaaagatt aagagaagag cgtgaacgac taggattcag ccaagctgat 60
tttgctgagt tagttggtgc atcaaggaaa tcgcaaataa ggtgggaaaa agctgaatca 120
gctcctagcg ctgattcaat aagtctgtgg gccaatgttg gcttagacgt gttgtatctg 180
atcactgggc aagctcagaa atcgtcatca aagacacaaa aaagtagtga aatagatgaa 240
attctactgg gtaagattgt tcataaactg gacttattag cgaaacatgc caatcgcaga 300
tggacaccta gcgaactcgt tttacagtca tcgaaaatat ataacttttt aatcaacgag 360
tcggtagttg atgacgatag gatcgataac gtgcttaaac tggttgttaa taattaa 417
<210> 138
<211> 492
<212> DNA
<213> 噬菌体
<400> 138
atgcgattca gtgacaacgg tctaagattt acggcagcat gggaggcttt cagcccggtg 60
ccgtatttcg cgaccaagaa agagcaggcc cgcggcctgt acacttgggg ttatggtcat 120
accggcacta atccgcctcg aagcattacc cgcgcggaag cgctggagct actcaagcgc 180
gatgtggcgt atgccgagaa ctgggtgaac aaatacgcac atccgagcat taatcaggcg 240
caattcgacg cgctggtgga cctggtaatc aatgctggtc caggtccgat cgtaccggac 300
catgtcgcga acgatttcga tgatgcggtg cgactgggtg actgggcgaa ggtccgggct 360
atactgccgc agttccgcaa gcaaggcggg tcggtgctct taggtctggt acgtcgcgca 420
atcggcagac aggcgctatt cgatggtaag cagtgggacg ttgccgagaa aatcgggcgt 480
aacgcagcat aa 492
<210> 139
<211> 414
<212> DNA
<213> 噬菌体
<400> 139
atgagtttta aatttggtaa aaatagcgaa aaacaattag ctaccgttaa gccagagcta 60
caaaaagtag ctcgtagagc tttagaatta tctccatatg attttacgat cgtacagggt 120
attcgtacag tagcacaaag tgcccagaat attgctaatg gtacttcatt tttaaaggat 180
cctagtaaaa gtaagcatat tactggggat gctcttgatt ttgctccata cattaatggc 240
aaaattgatt ggaatgactt agaagcattt tgggcagtta aaaaggcttt tgaacaagct 300
ggtaaagaac taggcattaa gcttcgtttt ggtgctgatt ggaatgcttc gggagactat 360
cacgatgaaa taaaacgtgg tacctatgat ggcggtcatg tcgaactagt ttaa 414
<210> 140
<211> 489
<212> DNA
<213> 噬菌体
<400> 140
atgaatatat ttgaaatgct tcgtaatgac gaaggtctta gactgacttt atacaaagac 60
actgaaggct tttggacgat tggcataggc catttagtaa caaagaaccc gtctttagcc 120
gtagctaaag ctgaacttga cagaatgatc ggtcgtaaat gcaacggtac aattaccctt 180
gatgaggctg aaaagctatt taatgaagac gttgataaag ccgtccgcgg aatcttgggt 240
aatgctaaac ttaaaccggt atatgattct ttagatgcag ttcgtcggtg tgcattggtc 300
aatatggtct tccaaatggg tgtagcaggc gtcgctggtt ttactaattc tcttcgtatg 360
cttaaacaga aacgttggga tgaagcggca gtaaatctag ctcaatctaa atggtatcgt 420
cagacaccta atcgcgcgaa acgcgtaatc tcaacattta aaacaggaac ttggaaagcg 480
tatatatga 489
<210> 141
<211> 465
<212> DNA
<213> 噬菌体
<400> 141
atgcaactct caagaaaagg tttagaagct attaagttct ttgaaggtct taagctagag 60
gcttacgaag attctgctgg aatccctaca attggatatg ggactatccg tattgctggt 120
aagcctgtta agatgggaat gaaaatcacc gctgaacaag ctgaacagta tcttctcgcg 180
gatgttgaaa agttcgttgc agcagtgaac aaagctatca aggttccaac ttctcagaat 240
gagttcgatg cacttgtaag tgaaacatac aacatcggta tcacagctat gcaggactca 300
acatttatca agcgccacaa cgatggtaat aaggtaggtt gtgcagaagc tatgcagtgg 360
tggaacaaag ttacagtcaa aggtaagaag gtcacttcaa acggcctgaa aaacagacgt 420
aggatggaag ctgacattta tcttgacagt gtatatccaa agtaa 465
<210> 142
<211> 486
<212> DNA
<213> 噬菌体
<400> 142
atgcgattca gtgacaacgg tctaagattt acggcagcat gggagcaatt cagcccagtg 60
ccgtatttcg cgactaagaa agagcaggcc cgcggcctgt acacctgggg ttatggtcat 120
accggcacca acccgcctcg aagcattacc cgagcggaag cgctggagct actgcgcaag 180
gatattgccg tagcagagac gtttgtgcag cgcgcaatgc cgatcggggc attcaaccaa 240
gcccagtacg atgcgctcgt agacctggtg ttcaacgtag gtccgcgtgc aatcggcgcg 300
aatactggaa ctggtcgcgc cgttcgtgct ggggatgttc agacgatccg tgttaaactg 360
ccacaattcc gtatgcaagc cggtgtagtt gttaagggtc tggtgcgtcg cgctaatgga 420
cgccttgcgc tattcgacgg aaagcagtgg gacgttgccg agcgtatcgg tcgtaattca 480
gcgtaa 486
<210> 143
<211> 498
<212> DNA
<213> 噬菌体
<400> 143
atggacattt ttggtatgct tcgtattgac gaaggctgta aattagaatt atacaaagac 60
actgaaggtt tttggactat tggcatagga caattaatta ccaaaaaccc ttcatacaat 120
gtagcacgtg atgagcttga tcgattaatg ggtcgggttt gtaatggtcg tatcactcaa 180
caggaagcag aaaatctttt caattcttct gtagaaaaag ctcgtaaagg tatcttggct 240
aatgctactc taaaacctgt ttatgatgtg ctagatgaag ttcgtcgttg tgcgttaatt 300
aacatggtgt tccagatggg tgttgccggt acagcaggtt tccctaaagg tatgcgtctg 360
ttaaaagcga aacagtggga caaagccgca atcgagttag ctgattctcg ctggtacaaa 420
tctcaaaccc ctaatcgcgc caaacgcgtg attgcaactt ttaaaactgg tacttggaaa 480
gcatatgaaa acctataa 498
<210> 144
<211> 885
<212> DNA
<213> 金黄色葡萄球菌
<400> 144
acacgtttaa caacacaaga attattatat ctaaatgaac taaaattagc aataccttgt 60
aaataaaaaa tgtttatatt tttcactatt atagagctat ttatctaaaa aggttcaata 120
agacttaaat acgaattcag gcaacttaat tgtgttaaat acagttttga atgcctaact 180
gtatttcttt tctctttaaa atacagttaa gtacattata agatgttgtg cggataaaca 240
aactaattgc atcaaattta ttttaaaata acaacaacaa aacgttaaga gaataacatt 300
tcggtgattt aaaagctacg cacgtttttg ttatcttcaa atttaaattt taaggagtgt 360
tttcaatgga aggtttattt aacgcaatta aagataccgt aactgcagca attaataatg 420
atggcgcaaa attaggcaca agcattgtga gcatcgttga aaatggcgta ggtttattag 480
gtaaattatt cggattctaa tttcaatatg ttatgtaagt aatcagtatt atttcaaagg 540
tgagggagag atttaaatga ctggactagc agaagcaatc gcaaatactg tgcaagctgc 600
acaacaacat gatagtgtga aattaggcac aagtatcgta gacatcgttg ctaacggtgt 660
gggtttacta ggtaaattat ttggattcta atataataac taatattctt taaaataaac 720
tgggtgagca tactttaatg ttatgcactc agtttatttt atttgcagaa atttgagcct 780
ctgttaagat ttagatacat agacaatata ggagatgggg aaattgggat ataaaaatat 840
tttgatagac tttgatgata caattgttga tttttatgat gcaga 885
<210> 145
<211> 1395
<212> DNA
<213> 细菌
<400> 145
atgaccatcg acgttgccat cgcctttgcc gacgctaacc actccaccta cctcgaagac 60
ctgaaaggcc tcgcgcgcat accgagcgtc agcttcccgg gcttcgacgc tgccgaagtg 120
gagcggtccg cggaacacgt cgcggacctg tgccggaatc gtggcctcga gaacgtcgaa 180
gtccttcgcg tccccggcgc ccacccctac gtctacggcg actggctaca tgcgccaggc 240
aagccgacgc tgcttctcta cgcgcaccac gatgtgcagc cgccgggccg cgaggagctg 300
tggctcacgc cgcccttcga gccgacggag cgcgatgggc gcctgtacgg ccgcggctgc 360
gctgatgaca aagccggcgc tatcacgcac atcgctgcga ttgcagcgtg gctgggcgcg 420
accggctcgc tgccgctgaa cgtgaagctg atcatcgaag gggaggaaga gatcggctct 480
gagcaccttg agtcgtttct ggaaacccat gcttcgaagc tgatggcgga cgccatcgtg 540
ctcaccgaca ctgccaactt cgatgccgac acgccgtcga tcacagtggc cctccgcggc 600
cttgtcgcag tcgacgtcac ggtgcggtcc atggaccatc cattgcactc cggcctgtgg 660
ggcgggccga taccggaccc ggtgcaggcc ctcgcccgca tgattgccgc ctgcactgac 720
gcgacggggc ggatgaccat ccccggcata tacgacgacg tcgcggagcc cggcgctgcc 780
gccgaggcaa gcctcaaggt gctggagtac cctgaagcgc tcttccgcga gcacaccggc 840
ctcaaccctg agtcgcagct actggtggcg tctacagacg tcctgcgctc gaactggtac 900
cagccgtcgc tctccgtgaa cgccatccag gcttcgtcgc gcagggacgt cgcgaacatc 960
atcaacgaca gtgcctgggc gcacgtcggc atccgcatcg tgcccaacat ggacgcgcgc 1020
aagaccctcg aggcgctgaa agcgcacctc gcagctaatg cgccgtgggg cgtgcgcgtc 1080
gaattctcca acgaagccgc cagccccgcc tggaccacgg ataccgacgc accggccttc 1140
gcagccgccc gccgcgccct cgaacgcggc tacgccaagc ccgccatcga catgggctgc 1200
ggcggctcca taccctttgt cggccccttc gccgctgccc tcaagggcgc cccggccctg 1260
cttatcggcg tcgaagaccc gctgtcaaac gcccacagcg agaacgagag cctgcatctc 1320
ggcatgttcc gcaaagcaat tgcgtcggcg gtagcgctgt acggagaaat cgcggcgcta 1380
gacagcgtga agtag 1395
<210> 146
<211> 1029
<212> DNA
<213> 细菌
<400> 146
atggccgacc gcagcgacgt ggcaccccga cctccgcggg cccggaccgg cgacgccatc 60
cgcgtgcccg cggccgcgct cgccagcgcg gtcgccgcgc tgggccgccg ggcggtcggc 120
cggaagcggc ggcccgcctg gagcgcgcgg ctggaagcca ccatcgccgc ctaccgcgga 180
tcgtggtcga ccatgcccgc gctcgggatc gtgcgctggc gcaacgtcgg cgaagcgctg 240
agccgcctcc gcacggacgg cctccgcccg cgcttcaccc gcttcgagag cggccagtac 300
ggcatccagg gcacctggct cgagccggcc ggcgccagtg gggccgtgct gctctacttc 360
cacggcgggg gcttctgctt cggctcgatc cggacccacg gtccgctcat cggcgcgctg 420
gcccgggcgg cgcgggcgcg cacgttcgcc gccgagtatc gcctcgcgcc cgagcacacg 480
gcgcccgccg cccacgacga cgcgctgtcg gcctaccggc acctgctcgg tgaaggcatc 540
gcgccgcggc agatcgtgct cgccggcgat tcgtccgggg gcacgctggt cctcagcacg 600
ctgctggcgc tgcgcgacgc cggggaggcg atgccagcgg ccggcatcgc catctcgccc 660
tgggtggacc tgtcgtgctc gggcgcgagc ttcaaggcca acgccccctt cgacttcgtc 720
ggcgagccgc actgtcggct ggcggcggtc agctatctgg gcgccgtcga tccgcggcgg 780
cccgacatct cgccgctgtt cgccgacctg cgcggcctgc cgccgctgct gatccaggcc 840
ggcggcgccg aggtcctggt cgaccagatc cgggcgttcg cgggtcgcgc ccgcgacgcc 900
ggcgtcgacg tcacgctctc ggtctacgac gacatggtgc acgtgtggca cctgatgcgc 960
gacgtcacgc gggacggcca gcgggccatc gacgaggccg ccgcgttcgc gcgcgcccac 1020
gcctcgtga 1029
<210> 147
<211> 1086
<212> DNA
<213> 细菌
<400> 147
ttgctgttcc gcctcctcgc cgtcgccgac ggacacaccc cggccttgac cccgaaactc 60
gtggacccca gggccgggcc ggtgaccttc aagctcgccg cgcatgacct cgacaagcgt 120
gaccccgccc tggtgctcaa gggccgcgtc ctcgacgagg acaggaagcc cgtggccggc 180
gccgtggtcg agccctgggg gttccagaaa gggcagcggg gtcagggcgg cgggctgaag 240
gggttcgacc atctcgccgt caccaacaac aagggggagt tccggctggg cgtccccgag 300
cccgggttag aggtatgggt ccaggtcggg gcgcggttca tggccgccca gaaagcatgg 360
ctccttgccg gccccaaggc gcatgacttg accctcgtca ccggcgcctc cgtcaccggc 420
cgcctgctga aggacggcaa gccgctcgcc ggcgtcgcgg tcgggctggc gcagaagagc 480
cgcgacccct tcaccttcgt cggcgacttc caggcggcca ccgatgagat gggtgtcttt 540
cgcatcccgc acgtcctgcc gagagtcgag tacttcctct acggcaagat ggacagcctg 600
cgggcacacg gggccatcgc ggtgaaggag gtgcggaccg gcgccagcgg ggcggaggtg 660
gatgtgggcg acctggccgt ggcccctggg cacaagctga cgggccaggt ggtcctggcg 720
gacgacaagc cggtgcccga aggggcgagg gtgctcgtgt cgcgcgagga ggcgtgggac 780
acgcagcagg cggtcgtcga caaggatgga catttcgcgt tggaggggct gccgccggag 840
cggtattccc tctcggtgcg ggtgcccggc taccgcctct cgccgaagaa cggcagctac 900
gacctgctgg gcggtttccg cctgctgggt gtggtccgct cggacatcaa gtcgctgcgg 960
gtcctgctgg agaaggggga ggggccggac cgacttccca catttgacca gaagcggcac 1020
aaggagtacc aacgcctccg ggacgcgccc ttgcgcgggg ccccggaaga gggccgcgcc 1080
aagtga 1086
<210> 148
<211> 483
<212> DNA
<213> 细菌
<400> 148
atgaaaaaat taatggcaac cctgattctg gcaggcttgg caacttccgt atctgcagcc 60
ggtatccata ttgaagacgg ttgggcacgt gccactgtcg aaggtatgaa aatgggtggc 120
gcattcatga aaatccaaaa cgatgaagcc aaacaagact tcttggtagg tggcagcagc 180
cctgttgcag aacgcgttga agtacacacc catattaacg acaacggtgt tatgcgtatg 240
cgcgaagtta aaggcggcgt gcctttggct gcaaaaggcg taaccgagct gaaaccaggc 300
agctaccacg ttatgttcat gggtttgaaa aaacaattga aagaaggcga aaaagtgcct 360
gtaaccctga aattcaaaaa cgcgaaacct caaactgtcg agctggaagt gaaaaccgca 420
ccacaatccg gtatggatca cggtcacgac cacggtgcac acggtggcgc acatcagcac 480
taa 483
<210> 149
<211> 828
<212> DNA
<213> 家蚕
<400> 149
acattctgtc tcgagcagcg aaacctgtca caattcaaaa tgtattccaa ggtgttgtta 60
tccgctgcac tccttgtatg cgtgaacgct caagtttcta tgcctcctgg ttacgcagag 120
aagtatccga tcaccagcca attttcaagg tcagtccgac accctcgcga tattcacgac 180
tttgtcactt gggacaggga aatgggggga gggaaggtct tcgggacttt gggagagagc 240
gaccaaggac tttttggtaa aggtggttac aacagggagt tcttcaatga tgaccgcggc 300
aaactgaccg gacaggctta cggcaccaga gtattaggac ctggaggcga cagtaccagt 360
tacggtggtc gtctagactg ggccaatgag aacgccaagg ctgctattga cttgaacagg 420
caaattggtg gcagcgctgg gatcgaagca tcagcttccg gcgtgtggga tcttggtaag 480
aatactcact tgtcagctgg cggagtggtc tctaaggagt tcggtcacag aaggcctgat 540
gtcggtttac aggcccagat tactcacgag tggtaattgc caacagcatt atcaaagcag 600
ctatataagt gccgcttaga caagcattct tgcctcgtaa gcctagaagt atgaataaaa 660
ttttgtctaa aaacattgtt ataaaaatcg attgatttgt aactatttat ctgcgttctg 720
taatttcctc tttaaatcac caacattcat ctaacatttt ttaaatatga ataaaaataa 780
atttcttaat ataaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaa 828
<210> 150
<211> 805
<212> DNA
<213> 家蚕
<400> 150
acattcggtc ttgaggagcg aaacttgata caactcaaaa tgaatacaaa tctgttttat 60
atcttcgcta caactctggt gtgtgttaac gcagaagttt acggaccttc tgattacgcg 120
gaagattact cgatcagcgg gcaatcctca aggcgacacc ctcgtgacgt cacttgggac 180
aaacaaatgg ggggaggcaa ggtctttggc actttgggac aaaacgatga tggactcttt 240
ggaaaagctg gttacaacag agagatcttt aatgacgacc gcggcaaact aaccggtcag 300
gcttacggca ccagagtttt aggacctgga ggcgacagca ccaactacgg cggacgccta 360
gactgggcga acaagaatgc acaagccact attgacctaa atagacaaat cggtggcaga 420
tctgggatga cagcatcagg ctccggtgtg tgggatctgg ataagaacac ccacttttct 480
gccggtggta tggtctcgaa ggagttcggt cacaaaagac cagacgtcgg tcttcaagca 540
gagatccgcc atgattggtg atcacctcaa cacttaaaca ataatctttt gccaaataaa 600
ttagtttttt ttaattattc aatataggtt attattgatt gttttatcta aaatcgaaat 660
ttacgaagta tgtctaactg taagtttgtt tcataatgta attagacaaa taggatctcg 720
tgctttgtta tgacggttag aagtacaatt taataaaaaa attacgtttc ctaaaaaaaa 780
aaaaaaaaaa aaaaaaaaaa aaaaa 805
<210> 151
<211> 879
<212> DNA
<213> 家蚕
<400> 151
acattctgaa ttaactagcc aaaccacaaa caactcaaaa tgaattccaa attgctgttt 60
ttcatcgcta cagtcctggt gtgtgtaaac gcagaagtat acaggtctcc tgattacgaa 120
gaagaatacc ccatcagagg cctattttca aagcgacacc cccgtgacgt cacgtgggac 180
acgaaaatgg gaggagggaa ggtcttcggc actttgggac agaacgacga tggactcttc 240
ggtaaagccg gttacaacag agagattttc aatgatgacc gcggtcaact aaccggccag 300
gcttacggca ccagggtctt aggacccgga ggtgacagca ctaactacgg tggacgtctg 360
gactgggcca acaagaacgc acaagccgcc attgacataa acagacaaat cggaggcaga 420
tctggaatga cagcctcggg ctccggtgtg tgggatcttg ataagaacac ccacatctca 480
gccggcggta tggtctcgaa ggaatttggt cacagaagac cagacgtcgg tcttcaagca 540
gagatccggc atgagtggtg attgtcaaca caatcccatc attcatagaa ttatgtttgt 600
ctttcgccaa aaaaagctac tatttttttt ctgtgtatat tttgtaatta ctagtgtaaa 660
atgttatttt tgattgattt aagaaaatgt caatattatt aacgaaatat gtcttacata 720
ttatgtactg ttaacttgtt ttcttaatgt aattatgcaa acagtatttt gctttatctc 780
atactttgtg ataaattaat taaaccatct agataataaa taataataat aaaaaattaa 840
tagtttaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaa 879
<210> 152
<211> 840
<212> DNA
<213> 家蚕
<400> 152
acattctgtc tcaagcagcg aaacctgaca caagtcaaaa tgaattccaa actattatat 60
ttcttcgcca cggtcctggt gtgtgtcaac gcagaagttt attgggagga cgaagaagga 120
tatccagtta gcggtcagtt ttcaaagcga cacccgcgtg acgtcacctg ggacaaacaa 180
gtggggggag ggaaggtctt cggcacgctg ggccaaaatg atgatgggct ttttggtaaa 240
gccggttaca acagagagat cttcaatgat gaccgcggca aattgaccgg gcaggcctac 300
ggcaccaggg tcctgggacc cgcaggggac agtacaaact acggtggacg tctagactgg 360
gccaataaga atgcggaagc tgctattgat ataaatagac agatcggagg cagatctgga 420
atgacagcaa caggctcagg agtgtgggat cttgacaaga acacccgcct ctcagccggc 480
ggtatggtct cgaaggaatt cggtcacaga agaccagacg tcggcgtcca ggcagagttc 540
cgtcatgatt ggtgatcaag aagatcccat caagacatca tagatctaaa caataatctt 600
ttgccaaaga aatcgatact ttcactctgc atttttttaa attactaaga actgaatcaa 660
attttagaat tattataatt aaagttatat attttagtga taaattattt ggataatgta 720
agtaaataga tatagttaca gtattctact ttacttgtac ttttaataaa acaaattgaa 780
ttcaatggaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 840
<210> 153
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 多克隆位点
<400> 153
gccggcagtg gatccccggg gaagatattc 30
<210> 154
<211> 15
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 154
gctacactgc tgaga 15
<210> 155
<211> 15
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 155
tctcagcagt gtagc 15
<210> 156
<211> 13
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 156
catcagatgc tgg 13
<210> 157
<211> 13
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 157
ccagcatctg atg 13
<210> 158
<211> 35
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 158
taatactcta cagacaccac taactgatgc tgctg 35
<210> 159
<211> 54
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 159
ctcgtttcaa catcttttat tttgtacata caagggatta agcagttctt accc 54
<210> 160
<211> 54
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 160
ctcgtttcaa catcttttat tttgtacata caagggatta agcagttctt accc 54
<210> 161
<211> 29
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 161
atgtacaaaa taaaagatgt tgaaacgag 29
<210> 162
<211> 32
<212> DNA
<213> 人工序列
<400> 162
caccccttaa ttaaataaag tgtattaggg tc 32
<210> 163
<211> 53
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 163
cactttattt aattaagggg tgatgactga ttgttaagat ggtgttaata ttc 53
<210> 164
<211> 29
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 164
atgtacaaaa taaaagatgt tgaaacgag 29
<210> 165
<211> 33
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 165
tcacccctta attaagtaaa gtgtattagg gtc 33
<210> 166
<211> 55
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 166
agaccctaat acactttact taattaaggg gtgatgactg attgttaaga tggtg 55
<210> 167
<211> 33
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 167
gtggtaaggt aaggtatgga aggatggcag tag 33
<210> 168
<211> 1173
<212> DNA
<213> 葡萄球菌噬菌体SP5
<400> 168
atgtacaaaa taaaagatgt tgaaacgaga ataaaaaatg atggtgttga cttaggtgac 60
attggctgtc gattttacac tgaagatgaa aatacagcat ctataagaat aggtatcaat 120
gacaaacaag gtcgtatcga tctaaaagca catggcttaa cacctagatt acatttgttt 180
atggaagatg gctctatatt caaaaatgag ccccttatta tcgacgatgt tgtaaaagga 240
ttcattacct acaagatacc taaaaaggtt atcaaacacg ctggttatgt tcgctgtaag 300
ctgtttttag agaaagaaga agaaaaaata catgtcgcga acttttcttt caatatcgtt 360
gatagtggta ttgaatctgc tgtagcaaaa gaaatcgatg ttaaattggt agatgatgct 420
attacgagaa tcttaaaaga taacgcgaca gatttattga acaaagactt taaagagaaa 480
atagataaag atgtcatttc ttacatcgaa aagaatgaaa gtagatttaa aggtgcgaaa 540
ggtgataaag gcgaaccggg acaacctgga gcaaaaggtg aagtaggtaa aaaaggagaa 600
caaggcgcac ccggtaaaaa cggtactgta gtatcaatca atcctgacac taaaatgtgg 660
caaattgatg gtaaagatac agatatcaaa gcagaacctg agttattgga caaaatcaat 720
atcgcaaatg ttgaagggtt agaagataaa ttgcaagaag ttgaaaaaat caaagataca 780
actctcaacg actctaaaac gtatacggat acaaaaattg ctgaactagt tgatagcgcg 840
cctgaatcta tgaacacatt aagagaatta gcagaagcaa tacaaaacaa ctctatttca 900
gaaagcgtat tgcaacagat tggctcaaaa gttagtacag aagattttga gagattcaaa 960
caaacactaa atgatttata tgctccaaaa aatcataatc atgacgagcg gtatgttttg 1020
tcatctcaag cttttactaa acaacaagcg gataatttat atcaactaaa aagcgcatct 1080
caaccgacgg ttaaaatttg gacaggaaca gaaaatgaat ataactatat atatcaaaaa 1140
gaccctaata cactttattt aattaagggg tga 1173
<210> 169
<211> 1173
<212> DNA
<213> 葡萄球菌噬菌体SP6
<400> 169
atgtacaaaa taaaagatgt tgaaacgaga ataaaaaatg atggtgttga cttaggtgac 60
attggctgtc gattttacac tgaagatgaa aatacagcat ctataagaat aggtatcaat 120
gacaaacaag gtcgtatcga tctaaaagca catggcttaa cacctagatt acatttgttt 180
atggaagatg gctctatatt caaaaatgag ccccttatta tcgacgatgt tgtaaaagga 240
ttcattacct acaagatacc taaaaaggtt atcaaacacg ctggttatgt tcgctgtaag 300
ctgtttttag agaaagaaga agaaaaaata catgtcgcga acttttcttt caatatcgtt 360
gatagtggta ttgaatctgc tgtagcaaaa gaaatcgatg ttaaattggt agatgatgct 420
attacgagaa tcttaaaaga taacgcgaca gatttattga acaaagactt taaagagaaa 480
atagataaag atgtcatttc ttacatcgaa aagaatgaaa gtagatttaa aggtgcgaaa 540
ggtgataaag gcgaaccggg acaacctggt gcaaaaggtg aagcaggtaa aaaaggagaa 600
caaggcgcac ccggtaaaaa cggtactgta gtatcaatca atcctgacac taaaatgtgg 660
caaattgacg gtaaagatac agatatcaaa gcagaacctg agttattgga taaaatcaat 720
atcgcaaatg ttgaagggtt agaagataaa ttgcaagaag ttaaaaaaat ccaagataca 780
actctcaacg actctaaaac gtatacggat tcaaaaattg ctgaactagt tgatagcgcg 840
cctgaatcta tgaacacatt aagagaatta gcagaagcaa tacaaaacaa ctctatttca 900
gaaagtgtat tgcaacagat tggctcaaaa gttagtgcag aagattttga ggaattcaaa 960
caaacactaa atgatttata cgctccaaaa aatcataatc atgacgagcg gtatgttttg 1020
tcatctcaag cttttactaa acaacaagcg gataatttat atcaactaaa aagcgcatct 1080
caaccgacgg ttaaaatttg gacaggaaca gaaaatgaat ataactatat atatcaaaaa 1140
gaccctaata cactttactt aattaagggg tga 1173
<210> 170
<211> 34
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 170
aagactcgga agaaggtagt cactaaggaa agtg 34
<210> 171
<211> 56
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 171
ccgtaaatct tagaccgttg tcactgaatc gcatgtcaag ttttacatag aaatcc 56
<210> 172
<211> 38
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 172
atgcgattca gtgacaacgg tctaagattt acggcagc 38
<210> 173
<211> 37
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 173
ttatgctgcg ttacgcccga ttttctcggc aacgtcc 37
<210> 174
<211> 55
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 174
ttgccgagaa aatcgggcgt aacgcagcat aaaaggtgat gtgggtcttg atagg 55
<210> 175
<211> 35
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 175
gcaacactgt atcggctact tcaaagtctt ctctg 35
<210> 176
<211> 492
<212> DNA
<213> 噬菌体
<400> 176
atgcgattca gtgacaacgg tctaagattt acggcagcat gggaggcttt cagcccggtg 60
ccgtatttcg cgaccaagaa agagcaggcc cgcggcctgt acacttgggg ttatggtcat 120
accggcacta atccgcctcg aagcattacc cgcgcggaag cgctggagct actcaagcgc 180
gatgtggcgt atgccgagaa ctgggtgaac aaatacgcac atccgagcat taatcaggcg 240
caattcgacg cgctggtgga cctggtaatc aatgctggtc caggtccgat cgtaccggac 300
catgtcgcga acgatttcga tgatgcggtg cgactgggtg actgggcgaa ggtccgggct 360
atactgccgc agttccgcaa gcaaggcggg tcggtgctct taggtctggt acgtcgcgca 420
atcggcagac aggcgctatt cgatggtaag cagtgggacg ttgccgagaa aatcgggcgt 480
aacgcagcat aa 492
<210> 177
<211> 53
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 177
catatcctgc gccagtcgcg acatacaagg gattaagcag ttcttaccca agc 53
<210> 178
<211> 34
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 178
atgtcgcgac tggcgcagga tatgaaaaaa ctgg 34
<210> 179
<211> 27
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 179
tcaatcagta tacccgtata cctgctc 27
<210> 180
<211> 50
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 180
ttgagcaggt atacgggtat actgattgat gactgattgt taagatggtg 50
<210> 181
<211> 51
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 181
catatcctgc gccagtcgcg acatgtcaag ttttacatag aaatcctgtc a 51
<210> 182
<211> 34
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 182
atgtcgcgac tggcgcagga tatgaaaaaa ctgg 34
<210> 183
<211> 27
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 183
tcaatcagta tacccgtata cctgctc 27
<210> 184
<211> 52
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 184
ttgagcaggt atacgggtat actgattgaa aggtgatgtg ggtcttgata gg 52
<210> 185
<211> 34
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 185
acaaatagtg aagagataaa ccaggttgag caag 34
<210> 186
<211> 45
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 186
ttgacgttga atctggagtc gataggtgcg acaggttacc aatgg 45
<210> 187
<211> 41
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 187
gtcgcaccta tcgactccag attcaacgtc aaggtctcac c 41
<210> 188
<211> 35
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 188
ttccaagacg gattcgaacc gtcactagta caagg 35
<210> 189
<211> 47
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 189
ttaatgatgt tatctcgata acgtcgacat ggagactcag taaatgg 47
<210> 190
<211> 44
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 190
tctccatgtc gacgttatcg agataacatc attaaggttg tacc 44
<210> 191
<211> 49
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 191
ttgaataaac cgttatcgcc ttcttaaagc aacctgtatt gcgttctgc 49
<210> 192
<211> 50
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 192
ttgctttaag aaggcgataa cggtttattc aacaaaccct catttcattg 50
<210> 193
<211> 49
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 193
tctgtgtagt tcggccaact gtagtgtgcg aatgatgcag cgaacattc 49
<210> 194
<211> 50
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 194
ttcgcacact acagttggcc gaactacaca gataccatga agcagtactc 50

Claims (37)

1.一种将噬菌体工程化的方法，所述方法包括：
-鉴定噬菌体；
-分离所述噬菌体；
-从所述噬菌体的基因组中去除天然黏附基因；和
-在所述噬菌体的基因组中插入非天然黏附基因，其中，所述非天然黏附基因特异性用于黏附于所选择的细菌。
2.一种将噬菌体工程化的方法，所述方法包括：
-分离噬菌体；
-从所述噬菌体的基因组中去除所有黏附基因；
-插入编码一个或更多个黏附基因的第一独特开放阅读框，并插入编码一个或多个可用于克服细菌防御的基因的第二独特开放阅读框；
-在所述第一开放阅读框中插入非天然黏附基因，其中，所述非天然黏附基因特异性用于黏附于所选择的细菌。
3.如权利要求2所述的方法，其中，一个或多个可用于克服细菌防御的基因是细胞内溶素、生物膜减少剂、糖萼穿透剂或其任何组合。
4.一种将噬菌体工程化的方法，所述方法包括：
-分离噬菌体；
-从所述噬菌体的基因组中去除任何黏附基因；
-在所述基因组中插入多限制性酶盒；和
-在所述盒中插入非天然黏附基因，其中，所述非天然黏附基因特异性用于黏附于所选择的细菌。
5.一种将噬菌体工程化的方法，所述方法包括：
-分离噬菌体；
-从所述噬菌体的基因组中去除所有天然黏附基因；和
-在所述噬菌体的基因组中插入非天然黏附基因；
其中，所述非天然黏附基因特异性用于黏附于所选择的细菌。
6.一种使噬菌体生长的方法，所述方法包括：
-制备包含酵母和酵母营养物的酵母培养物；
-用噬菌体感染所述酵母；
-通过菌落PCR筛选所述酵母中的阳性转化子。
7.如权利要求1至6中任一项所述的方法，其中，所述噬菌体是裂解性噬菌体。
8.如权利要求1至7中任一项所述的方法，其中，所述去除步骤和所述插入步骤利用所述噬菌体的无细胞克隆。
9.一种噬菌体，其包含多限制性酶盒。
10.一种噬菌体，其包含非天然黏附基因，其中，所述非天然黏附基因特异性用于黏附于所选择的细菌。
11.如权利要求8至10中任一项所述的噬菌体，其中，所述噬菌体不包含天然黏附基因。
12.如权利要求8至11中任一项所述的噬菌体，其中，所述噬菌体是裂解性噬菌体。
13.如权利要求8至12中任一项所述的噬菌体，其中，所述非天然黏附基因对致病性细菌有特异性。
14.权利要求8至13中任一项所述的噬菌体在清洁、治疗或预防细菌污染中的应用。
15.如权利要求8至14中任一项所述的噬菌体，所述噬菌体还包含报告基因。
16.如权利要求8至15中任一项所述的噬菌体，其中，所述报告基因是绿色荧光蛋白基因。
17.权利要求8至16中任一项所述的噬菌体在诊断特定细菌存在或不存在中的应用。
18.一种产生突变体噬菌体的方法，所述方法包括：
使选定的噬菌体的黏附基因失活，所述选定的噬菌体分离自来自环境的噬菌体；
在选定的噬菌体中插入第一异源核酸序列，第一异源核酸序列包含编码第一特异性黏附基因的第一开放阅读框，第一特异性黏附基因不同于所失活的黏附基因并对所选择的细菌具有特异性，从而产生所述突变体噬菌体。
19.如权利要求18所述的方法，所述方法还包括插入第二异源核酸序列，第二异源核酸序列包含编码可用于克服细菌防御的基因的第二开放阅读框。
20.如权利要求19所述的方法，其中，所述可用于克服细菌防御的基因包含生物膜降解基因、糖萼降解基因、编码抗菌蛋白的基因、或破坏细菌壁的酶的基因中的一种或更多种，从而产生所述突变体噬菌体。
21.如权利要求20所述的方法，其中，所述破坏细菌壁的酶的基因是细胞内溶素。
22.如权利要求21所述的方法，其中，所述细胞内溶素包含核苷酸序列SEQ ID No：138；SEQ ID No：139；SEQ ID No：140；SEQ ID No：141；SEQ ID No：142；或SEQ ID No：143；或其片段。
23.如权利要求20所述的方法，其中，所述破坏细菌壁的酶的基因包含核苷酸序列SEQID No：144，或其片段。
24.如权利要求20所述的方法，其中，所述生物膜降解基因和糖萼降解基因包含Cathelicidin抗菌肽LL-37；组氨素3(HTN3)；乳酸链球菌素；分散素B；内切-1,4-β-葡聚糖酶(纤维素酶)；Aureolysin；NucB；丝氨酸蛋白酶(SspA)；LapG蛋白酶；蜂毒素；内切-1,4-β-甘露糖苷酶(manA)；或α-淀粉酶，或其片段中的一种或更多种。
25.如权利要求20所述的方法，其中，所述破坏细菌壁的酶的基因是靶向细菌细胞壁中的连接化学的基因。
26.如权利要求25所述的方法，其中，所述靶向细菌细胞壁中的连接化学的基因包含SEQ ID No：145；SEQ ID No：146；SEQ ID No：146；SEQ ID No：147；SEQ ID No：149；SEQ IDNo：150；SEQ ID No：151；SEQ ID No 152；或其片段中一种或更多种的核苷酸序列。
27.如权利要求18至26中任一项所述的方法，所述方法还包括筛选溶原基因，并使所述溶原基因失活。
28.如权利要求18至27中任一项所述的方法，其中，所述选定的噬菌体是溶原基因拷贝数低的噬菌体。
29.如权利要求18至28中任一项所述的方法，其中，所述失活步骤使所述选定的噬菌体的所有黏附基因失活。
30.如权利要求18至29中任一项所述的方法，其中，所述选定的噬菌体是裂解性噬菌体、具有小的基因组尺寸的细菌噬菌体、或具有裂解革兰氏阴性和革兰氏阳性细菌的结构和功能基因的噬菌体中的一种或更多种，或其任何组合。
31.如权利要求18至30中任一项所述的方法，其中，所述第一特异性黏附基因是黏附基因SP5、黏附基因SP6、或其片段。
32.如权利要求18至31中任一项所述的方法，其中，所述第一特异性黏附基因包含核苷酸序列SEQ ID No：125；SEQ ID No：126；SEQ ID No：127；SEQ ID No：128；SEQ ID No：129；SEQ ID No：130；SEQ ID No：131；SEQ ID No：132；SEQ ID No：133；SEQ ID No：134；SEQ IDNo：135；SEQ ID No：136；或SEQ ID No：137；或其片段。
33.如权利要求19至32中任一项所述的方法，其中，所述第一开放阅读框还编码不同于所述第一特异性黏附基因的第二特异性黏附基因。
34.一种突变体噬菌体，其根据权利要求18至33中任一项所述的方法产生。
35.一种用于对表面消毒或去污的抗微生物组合物，所述组合物包含权利要求18至34中任一项所述的突变体噬菌体。
36.一种对怀疑含有传染性细菌的表面去污的方法，所述方法包括施用杀菌有效量的权利要求35所述的组合物至所述表面。
37.一种消除微生物污染物的方法，所述方法包括：
获得由权利要求34所述的突变体噬菌体产生的一种或更多种裂解酶；
在无需事先用噬菌体对细菌污染物进行感染的情况下，将所述一种或更多种裂解酶施加于细菌污染物，以消除所述细菌污染物。
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