CN111771898A

CN111771898A - Safe and long-acting disinfection mother liquor

Info

Publication number: CN111771898A
Application number: CN202010652130.9A
Authority: CN
Inventors: 陈风雨; 许西洋; 张素琴; 刘润田; 孟婕; 李冬阳
Original assignee: Shanghai Mingyong New Material Co ltd
Current assignee: Shanghai Mingyong Technology Co.,Ltd.
Priority date: 2020-07-08
Filing date: 2020-07-08
Publication date: 2020-10-16

Abstract

The invention belongs to the technical field of disinfectants, and relates to a safe and long-acting disinfection mother solution which comprises a disinfectant, a water-soluble polymer and water, wherein the disinfectant is one or more selected from guanidine salt polymers. After the disinfection mother liquor is diluted into disinfection liquid, the disinfection mother liquor is coated or sprayed on the surface of an object to form an antimicrobial film. The disinfectant is enriched on the surface of the film and has hydrogen bond association with the water-soluble polymer, so that the disinfectant can effectively and durably exert a disinfecting function on the surface of an object at a very low concentration. The disinfection mother solution has the characteristics of safety and green, can efficiently disinfect the surface of an object for a long time, and has a good application prospect.

Description

Safe and long-acting disinfection mother liquor

Technical Field

The invention belongs to the technical field of disinfectants, and particularly relates to safe and long-acting disinfection mother liquor.

Background

On the surface of objects in hospitals, public health places, office areas, etc., some germs are often present and may be present for a long time (see table 1, yangming et al, modern hospital disinfection).

TABLE 1 survival time of certain bacteria on the surface of dry objects

Bacterial species	Time to live	Bacterial species	Time to live
				Acinetobacter	For 5 months	Helicobacter pylori	1.5h
Campylobacter jejuni	6d	Certain species of the genus Listeria	Several months
				Clostridium difficile spores	For 5 months	Mycobacterium tuberculosis	4 months old
Pseudomonas aeruginosa	5 weeks	Neisseria gonorrhoeae	3d
				Diphtheria bacillus	6 months old	Proteobacteria	24h
Escherichia coli	Several months	Salmonella typhi	4 weeks
				Genus enterococcus	4 months old	Salmonella typhimurium	Over 10d
Gold grape ball (with MRSA)	7 months old	Streptococcus pneumoniae	20d

To prevent cross-contamination, the surface of the object needs to be disinfected. Many types of disinfectants are available, including peroxides (peracetic acid, hydrogen peroxide, etc.), chlorines (sodium hypochlorite, chlorine dioxide, etc.), iodines (iodine and iodine preparations, iodophor disinfectants), aldehydes (formaldehyde, glutaraldehyde, etc.), alcohols (ethanol, isopropanol, etc.), phenols (phenol, p-chlororesorcinol, etc.), guanidines (chlorhexidine, etc.), quaternary ammonium salts (benzalkonium bromide, domiphen bromide, etc.). Various disinfectants have characteristics and also have problems, and typical problems of some disinfectants include: safety problems of the disinfectant in the preparation process, the use process and after use; off-flavors and irritation problems; short disinfection action time and long effectiveness.

In recent years, disinfectants containing guanidine groups have been used in increasing amounts, and there are biguanides and monoguanidines such as chlorohexidine (trade name: chlorhexidine), 2-ethylhexyl biguanide (trade name: aciridine), polyhexamethylene guanidine hydrochloride, polyhexamethylene guanidine phosphate and the like. Among them, the polyguanidine salt high molecular disinfectant has higher safety, mildness and sterilization performance. Guanidine salt disinfectants are well soluble and are often applied as aqueous solutions.

Chinese patent 200710041048.7 discloses an alcohol-containing polyhexamethylene guanidine iodine disinfectant, comprising: polyhexamethylene guanidine, 0.05-2, iodine, 0.1-5, alcohols, 30-80, a stabilizer, 0.1-2, a penetrating agent, 0.1-1, a skin care agent, 0.1-2, 10% phosphoric acid, 0.1-0.8 and the balance of water. The concentration of alcohols is relatively high.

Chinese patent 200910114010.7 discloses a skin disinfectant, which comprises polyhexamethylene biguanide hydrochloride and ethanol as main ingredients, wherein the content of ethanol is 50-60%, and the content of polyhexamethylene biguanide hydrochloride is 0.52-0.63%. The contents of ethanol and polyhexamethylene biguanide hydrochloride are both relatively high.

Chinese patent 201110151341.5 discloses a composition, which comprises polyhexamethylene biguanide hydrochloride and ethanol as main bactericidal components, and isopropanol as synergist, wherein the concentration of ethanol is 79-467 g/L, the concentration of polyhexamethylene biguanide hydrochloride is 0.01-15 g/L, and the concentration of isopropanol is 3-54 g/L. And isopropanol is a class 3 carcinogen (see world health organization international cancer research institute's list of carcinogens, 2017).

Chinese patent 201210370169.7 discloses a broad-spectrum pesticide aqueous emulsion, which comprises the following components in percentage by weight: 1-3% of abamectin, 20-35% of PHMG, 0.5-1.5% of high-water-solubility polyvinyl alcohol, 20-40% of organic silicon and 32-52% of kelp extract. The concentration of PHMG is very high.

Chinese patent 201210416266.5 discloses a medical disinfection gel, which comprises the following components in parts by weight: 1.0-5.0% of polyhexamethylene guanidine, 1.0-5.0% of thickening agent, 3.0-8.0% of humectant, 1.0-3.0% of triamine pH regulator and the balance of deionized water. The concentration of polyhexamethylene guanidine is high.

Chinese patent 201610340134 discloses a compound quaternary ammonium salt disinfectant, which is prepared by mixing hexamethylenetetramine, benzalkonium bromide, benzalkonium chloride, polyhexamethylene guanidine, octadecyl dimethyl benzyl chloride and tween 80 in proportion. The disinfectant has complex components, the content of the polyhexamethylene guanidine is more than 0.5 percent, and the content of the quaternary ammonium salt bactericide is higher.

The compound polyhexamethylene biguanide disinfectant of Chinese patent 201811360941.0 comprises, by weight, 0.2-0.5% of polyhexamethylene biguanide, 0.2-1% of citric acid, and the balance of water. The concentration of polyhexamethylene biguanide is high.

Chinese patent 201811370414.8 discloses a skin mucosa disinfectant for wound healing, which comprises the following components: 1.0-8.0% of honeysuckle extract, 1.0-4.5% of mint extract, 1.0-6.0% of sophora flavescens extract, 1.0-6.0% of angelica dahurica extract, 0.08-0.70% of polyhexamethylene guanidine (PHMG) and the balance of deuterium-depleted water.

The above patents all relate to the use of guanidinium polymers as disinfectants in aqueous solution. However, the problem of concern is the loss of the guanidinium polymer and the reduction of the disinfecting function by the loss, i.e., the problem that the functionality does not last. Also, the guanidine salt polymer content in the above patents is generally higher.

Disclosure of Invention

The invention aims to provide a safe and long-acting disinfection mother solution to solve the problems of safety and non-durability of a disinfection solution.

The disinfection mother liquor provided by the invention is diluted into disinfectant, and then disinfects the surface of an object, so that the disinfectant is safe, long-acting, simple and feasible, and has low consumption of effective disinfection substances and high efficiency.

The purpose of the invention can be realized by the following technical scheme:

the invention provides a safe and long-acting disinfection mother solution, which comprises a disinfectant, a water-soluble polymer and water, wherein the disinfectant is one or more selected from guanidine salt polymers.

The disinfectant in the disinfectant mother liquor is within 30 wt%, the water soluble polymer is preferably used in an amount (in weight) greater than that of the disinfectant, the water soluble polymer is limited in solubility in water, and the rest is water. The concentration of guanidinium polymer will depend on ease of use. For example, if the concentration of the guanidine salt polymer in the disinfection mother liquor is 1.0 percent (weight portion), the guanidine salt polymer can be diluted by 100 times and 200 times to be prepared into disinfection solution for use. The dilution factor may be higher if the concentration of guanidinium polymer in the disinfection mother liquor is higher.

The concentration of the water-soluble polymer in the disinfecting mother liquor cannot be higher than the solubility of the water-soluble polymer in water. In addition, the concentration of the water-soluble polymer is generally greater than the concentration of the guanidinium polymer, considering the association between the water-soluble polymer and the disinfectant in the present application, but the upper limit of the concentration of the water-soluble polymer is its solubility in water.

For example, when the water-soluble polymer is selected as soluble starch, it is reasonable to formulate the starch to a concentration of 10% (by weight) and at the same time facilitate dilution for use; if the water-soluble polymer is polyvinyl alcohol, the concentration of the polyvinyl alcohol in the disinfection mother liquor can be adjusted to 8% (weight parts), and the dilution use is also convenient.

The guanidine salt polymer contains more amino groups, the selected water-soluble polymer generally contains more hydroxyl groups or amino groups, and more and stronger hydrogen bond association action exists between the guanidine salt polymer and the water-soluble polymer.

In one embodiment of the invention, the safe and long-acting disinfection mother liquor further contains alcohol, and the concentration of the alcohol is 10-50% by weight, and preferably 15-40% by weight.

In one embodiment of the invention, the guanidinium polymer is selected from the group consisting of hydrochloride, phosphate, sulfate, nitrate, or carbonate salts of one or more of the following:

polyhexamethylene biguanide, polyethylene tetramethylene biguanide, polyethylene hexamethylene biguanide, polypropylene biguanide, polyaminopropyl biguanide, poly- [2- (2-ethoxy) -ethoxyethyl ] -biguanide ], polypropylene hexamethylene biguanide, polyethylene octamethylene biguanide, polyethylene decamethylene biguanide, polyethylene dodecamethylene biguanide, polytetramethylene hexamethylene biguanide, polytetramethylene biguanide, polypropylene octamethylene biguanide, polytetramethylene octamethylene biguanide, polyhexamethylene diethylenetriamine biguanide, polytetramethylene guanidine, polypentamethylene guanidine, polyhexamethylene guanidine, polyethylene hexamethylene guanidine, polypropylene guanidine, poly- [2- (2-ethoxy) -ethoxyethyl ] -guanidine, polypropylene hexamethylene guanidine, polyethylene hexamethylene guanidine, polypropylene hexa, Polyethylene octamethylene guanidine, polyethylene decamethylene guanidine, polyethylene dodecamethylene guanidine, polypropylene octamethylene guanidine or polyhexamethylene diethylene triamine guanidine.

In one embodiment of the present invention, the water-soluble polymer is selected from one or more of natural water-soluble polymers or synthetic water-soluble polymers.

Preferably, the water-soluble polymer is selected from biodegradable water-soluble polymers, and has the characteristics of safety, environmental protection and the like. The soluble starch is white or off-white powder, has no odor and odor, is insoluble in cold water, can be made into transparent solution in hot water, does not coagulate after cooling, and is derived from natural corn, potato, etc. In the disinfectant, starch is not generally adopted as a component of the disinfectant due to the nutritional function of the starch. The method takes the efficient guanidine salt polymer as a disinfection component, utilizes the adhesive property of the starch and stronger and more hydrogen bond association action between the hydroxyl of the starch and the amino of the guanidine salt polymer, takes the starch as a component of disinfection mother liquor, strengthens the binding force between the disinfection solution and the surface of an object on the one hand, and fixes the guanidine salt polymer through the hydrogen bond association action on the other hand, and because the guanidine salt polymer and the starch have different surface activities, the guanidine salt polymer is more distributed on the outermost surface of the system, so that the guanidine salt polymer can fully, efficiently and durably play a disinfection function on the surface of the object.

In one embodiment of the present invention, the water-soluble polymer may also be selected from one or more of the following: gum arabic, starch derivatives, carboxymethylcellulose, polyvinylpyrrolidone, polyvinyl alcohol, polyacrylamide or polyethylene glycol.

In one embodiment of the present invention, the water-soluble polymer is preferably a polymer that is relatively insoluble at normal temperature and relatively soluble at high temperature, such as: is selected from soluble starch and polyvinyl alcohol with high alcoholysis degree, so that the effect of the disinfection film formed on the surface of the object can be more durable.

In one embodiment of the present invention, the water-soluble polymer is preferably a polymer having good film-forming ability, such as: is selected from soluble starch, polyvinylpyrrolidone and polyvinyl alcohol, thus forming a layer of uniform film structure on the surface of an object and being easier to exert excellent disinfection function.

In one embodiment of the present invention, the water-soluble polymer is preferably a water-soluble polymer with a large molecular weight, such as polyethylene glycol (PEG), preferably with a small molecular weight (such as 8000), so that the surface of the object is more stable and durable.

In one embodiment of the invention, the water-soluble polymer is preferably a water-soluble polymer that is transparent after film formation, for example, soluble starch, polyvinyl alcohol are selected so that no change in its state is noticeable after spraying or coating of the surface of the object.

In one embodiment of the invention, the safe and long-acting disinfection mother liquor is selected from one of the following compositions:

1) the disinfectant comprises a disinfectant, a water-soluble polymer and water, wherein the disinfectant is polyhexamethylene guanidine hydrochloride or polyhexamethylene biguanide hydrochloride, the water-soluble polymer is starch, the concentration of the water-soluble polymer is 2.0-5.0%, and the concentration of the disinfectant is 0.1-5.0%;

2) the disinfectant comprises a disinfectant, a water-soluble polymer and water, wherein the disinfectant is polyhexamethylene guanidine hydrochloride or polyhexamethylene biguanide hydrochloride, the water-soluble polymer is polyvinyl alcohol, the concentration of the water-soluble polymer is 3.0-10.0%, and the concentration of the disinfectant is 0.1-7.0%;

3) the disinfectant comprises a disinfectant, a water-soluble polymer and water, wherein the disinfectant is polyhexamethylene guanidine hydrochloride or polyhexamethylene biguanide hydrochloride, the water-soluble polymer is a mixture of polyvinyl alcohol and soluble starch, the concentration of the polyvinyl alcohol is 2-5.0%, the concentration of the soluble starch is 3-5.0%, and the concentration of the disinfectant is 1.0-8.0%;

4) the disinfectant comprises a disinfectant, a water-soluble polymer and water, wherein the disinfectant is polyhexamethylene guanidine hydrochloride or polyhexamethylene biguanide hydrochloride, the water-soluble polymer is polyvinylpyrrolidone or polyethylene glycol, the concentration of the water-soluble polymer is 3.0-10.0%, and the concentration of the disinfectant is 0.1-3.0%.

5) The disinfectant comprises a disinfectant, a water-soluble polymer, alcohol and water, wherein the disinfectant is polyhexamethylene guanidine hydrochloride or polyhexamethylene biguanide hydrochloride, the water-soluble polymer is a mixture of polyvinyl alcohol and soluble starch, the concentration of the polyvinyl alcohol is 1-5%, the concentration of the soluble starch is 1-5%, the concentration of the disinfectant is 0.1-3.0%, and the concentration of the alcohol is 15-38%;

6) the disinfectant comprises a disinfectant, a water-soluble polymer, alcohol and water, wherein the disinfectant is polyhexamethylene guanidine hydrochloride or polyhexamethylene biguanide hydrochloride, the water-soluble polymer is soluble starch, the concentration of the soluble starch is 1-5%, the concentration of the disinfectant is 0.1-5%, and the concentration of the alcohol is 15-38%.

In one embodiment of the present invention, the water-soluble polymer does not suggest the use of polyanionic polymers such as polyacrylic acid, polymaleic anhydride, etc., or the addition of anionic-containing materials such as anionic surfactants in the subsequent dilution use, in order to avoid strong interaction between cations and anions, thereby causing instability of the disinfectant, based on the cationic property of the guanidine salt polymer.

The invention also provides a preparation method of the safe and long-acting disinfection mother liquor, which can be used for preparing the aqueous solution of the guanidinium polymer and the aqueous solution of the water-soluble polymer respectively and then uniformly mixing the aqueous solution of the guanidinium polymer and the aqueous solution of the water-soluble polymer in proportion. Or one of the components can be added into a certain amount of water according to a set proportion, and the other component can be added after the dissolution. The soluble polymer may be dissolved first.

The invention also provides a safe and long-acting disinfectant, which is prepared by diluting the safe and long-acting disinfectant mother liquor by adding water, wherein in the safe and long-acting disinfectant, the concentration of a disinfectant guanidine salt polymer is more than 10 ppm; further preferably, the concentration of disinfectant guanidine salt polymer is 10-5000 ppm; even more preferably, the concentration of disinfectant guanidinium polymer is from 10 to 500 ppm; still further preferably, the concentration of disinfectant guanidinium polymer is from 10 to 90 ppm. Of course, the concentration requirements of the guanidinium polymer in the disinfecting solution may vary due to different environmental conditions being disinfected, as some environmental conditions may require concentrations of the guanidinium polymer above 100 ppm.

The invention also provides application of the safe and long-acting disinfectant, and the safe and long-acting disinfectant is coated or sprayed on the surface of an object to maintain a long-term and excellent disinfection function of the surface of the object. The safe and long-acting disinfectant is coated or sprayed on the surface of an object, and in the process of volatilizing and drying the water on the surface of the object, the disinfectant is enriched on the surface of the outermost layer due to the surface activity characteristic of the disinfectant, so that the maximum disinfection function of the surface of the object is exerted by the least disinfection active substances.

In the invention, the guanidine salt polymer and the water-soluble polymer are used together, which can produce the following beneficial effects:

(1) and is safer. The guanidine salt polymer is not in a free state due to the association of hydrogen bonds with the water-soluble polymer, so that the safety is further improved.

(2) Is more efficient. The guanidine salt polymer has surface activity, so that the guanidine salt polymer tends to be distributed on the outermost surface in the gradual drying process of water volatilization after the guanidine salt polymer is sprayed or coated on the surface of an object in a disinfection system consisting of the guanidine salt polymer, the water-soluble polymer and water, and the disinfection function can be efficiently played on the surface of the object.

(3) Is more uniform. The water-soluble macromolecules can be uniformly distributed on the surface of an object and even form a film, so that the guanidine salt polymer is driven to be more uniformly distributed on the surface.

(4) Lower amounts are used. Because the guanidine salt polymer is enriched on the surface of the outermost layer, the object surface can have excellent disinfection function by using lower guanidine salt polymer.

(5) Has longer acting time. Because the guanidine salt polymer is combined with the polymer and the polymer has certain binding force with the surface of an object, the guanidine salt polymer can not be lost and the disinfection function can be maintained for a longer time.

The main reasons for the above beneficial effects are: the disinfectant mother liquor prepared from the guanidine salt polymer and the water-soluble polymer is diluted into disinfectant by water, and then sprayed or coated on the surface of an object, the guanidine salt polymer tends to be uniformly distributed on the outermost layer of the surface along with the evaporation of water and the gradual drying of the surface, and the guanidine salt polymer has stronger and numerous hydrogen bond association effects with the water-soluble polymer, so that the loss of the guanidine salt polymer is limited. That is, the guanidinium polymer tends to be distributed on a more surface layer due to surface activity, while the water-soluble polymer tends to be distributed on the surface contacting the surface of the object, enhancing the binding force with the surface of the object; because the guanidine salt polymer and the water-soluble polymer have stronger hydrogen bond interaction and a plurality of hydrogen bond interactions, the formed guanidine salt polymer disinfection layer is not easy to run off. Therefore, the guanidine salt polymer layer which is uniformly distributed and enriched on the outermost layer can be obtained, and the disinfection function of the guanidine salt polymer can be fully exerted. Therefore, excellent disinfection effect can be obtained by a smaller amount of the guanidine salt polymer, and the method is an economical and efficient use mode of the guanidine salt polymer; meanwhile, due to the interaction between the guanidine salt polymer and the water-soluble polymer, the loss of the guanidine salt polymer is prevented, so that the existence of free guanidine salt polymer can be avoided, and the long-term disinfection function is exerted. Due to the lower dosage and the uniform distribution, better use experience can be achieved.

The disinfectant liquid generally does not contain natural nutrient substances such as starch, cellulose and the like required by bacteria. Based on the synergistic effect between polymers such as water-soluble starch and the like and the guanidine salt polymer and the binding capacity between the water-soluble polymer and the surface of an object, the guanidine salt polymer is matched with the water-soluble starch or cellulose, or the guanidine salt polymer is matched with water-soluble polymers such as polyvinyl alcohol and polyethylene glycol, or the guanidine salt polymer is matched with a natural water-soluble polymer/synthetic water-soluble polymer compound, so that safe and efficient disinfection mother liquor is developed, and the compound disinfectant has the characteristics of nature, greenness, safety, environmental friendliness, small using amount, high-efficiency disinfection and the like. After being diluted by water, the disinfectant is coated or sprayed on the surface of an object, and under the condition of not changing the surface characteristics of a substrate, the disinfectant provides protection for as long as possible to avoid microbial pollution so as to reduce the using amount of the disinfectant and the disinfection operation times, and is friendly to the natural environment.

The invention provides simple, safe and long-acting disinfection mother liquor, after the disinfection mother liquor is diluted into disinfectant by water, the dosage of the disinfectant is reduced due to the synergistic effect of the disinfectant and the water-soluble polymer, and the long-term and high-efficiency disinfection function is maintained due to the interaction of the disinfectant and the polymer, so that the disinfection times and the dosage of the disinfectant are reduced, and the environmental health and the green development are maintained.

Detailed Description

The present invention will be described in detail with reference to specific examples.

Example 1

Adding 50 g of soluble Starch (Starch) into 850 g of water, stirring, dissolving, heating to 90 ℃ for further dissolving, supplementing water to the total amount of 900 g, and preparing a Starch water solution. 10 g of polyhexamethylene guanidine hydrochloride (GP1) is taken and added into 90 g of water, stirred and dissolved to prepare the guanidino polymer water solution. Adding 100 g of prepared guanidino polymer aqueous solution into 900 g of prepared Starch aqueous solution, stirring and uniformly mixing to obtain Starch/GP1-1 composite disinfection mother liquor with the Starch concentration of 5.0% and the guanidino polymer concentration of 1.0%.

Example 2

Adding 50 g of soluble Starch (Starch) into 850 g of water, stirring, dissolving, heating to 90 ℃ for further dissolving, supplementing water to the total amount of 900 g, and preparing a Starch water solution. 5g of polyhexamethylene biguanide hydrochloride (GP2) is taken and added into 95 g of water, stirred and dissolved to prepare the guanidino polymer aqueous solution. Adding 100 g of prepared guanidino polymer aqueous solution into 900 g of prepared Starch aqueous solution, stirring and uniformly mixing to obtain Starch/GP2-2 composite disinfection mother liquor with the Starch concentration of 5.0% and the guanidino polymer concentration of 0.5%.

Example 3

Adding 20 g of soluble Starch (Starch) into 880 g of water, stirring, dissolving, heating to 90 ℃ for further dissolving, and supplementing water to the total amount of 900 g to obtain a Starch aqueous solution. 1 g of polyhexamethylene guanidine phosphate (GP3) is taken and added into 99 g of water, stirred and dissolved to prepare the guanidinium polymer water solution. Adding 100 g of prepared guanidino polymer aqueous solution into 900 g of prepared Starch aqueous solution, stirring and uniformly mixing to obtain Starch/GP3-3 composite disinfection mother liquor with the Starch concentration of 2.0% and the guanidino polymer concentration of 0.1%.

Example 4

Adding 50 g of soluble Starch (Starch) and 50 g of polyhexamethylene guanidine hydrochloride (GP1) into 900 g of water, stirring, dissolving, heating to 90 ℃ for further dissolving, and supplementing water to 1000 g of the total amount to obtain the Starch/GP1-4 compound disinfection mother liquor with the Starch concentration of 5.0% and the guanidine salt polymer concentration of 5.0%.

Example 5

100 g of PVA with the brand number of 1799 is taken and added into 890 g of water, stirred, heated to 90 ℃, dissolved, and added with a small amount of evaporated water to reach the total amount of 990 g to prepare the PVA aqueous solution. And adding 2 g of polyhexamethylene guanidine hydrochloride into 8 g of water, stirring and dissolving to obtain a guanidino polymer aqueous solution.

Adding 10 g of prepared guanidino polymer aqueous solution into 990 g of PVA aqueous solution, stirring and uniformly mixing to obtain PVA/GP1-1 composite disinfection mother liquor with PVA concentration of 10% and guanidinium polymer concentration of 0.2%.

Example 6

100 g of PVA with the brand number of 1788 is taken and added into 800 g of water, stirred, heated to 90 ℃ for dissolution, and a small amount of evaporated water is added to the mixture until the total amount reaches 900 g, thus obtaining the PVA aqueous solution. 30 g of polyhexamethylene guanidine sulfate (GP4) is taken and added into 70 g of water, stirred and dissolved to prepare the guanidinium polymer water solution.

And adding 100 g of the prepared guanidino polymer aqueous solution into 900 g of PVA aqueous solution, stirring and uniformly mixing to obtain PVA/GP4-2 composite disinfection mother liquor with PVA concentration of 10% and guanidinium polymer concentration of 3.0%.

Example 7

Adding 50 g of PVA of 1799 brand into 850 g of water, stirring, heating to 90 ℃, dissolving, adding a small amount of evaporated water to reach a total amount of 900 g, and obtaining the PVA aqueous solution. And adding 30 g of polyhexamethylene guanidine hydrochloride into 70 g of water, stirring and dissolving to obtain a guanidino polymer aqueous solution. And adding 100 g of the prepared guanidino polymer aqueous solution into 900 g of PVA aqueous solution, stirring and uniformly mixing to obtain PVA/GP1-3 composite disinfection mother liquor with the PVA concentration of 5.0% and the guanidinium polymer concentration of 3.0%.

Example 8

Adding 50 g of PVA of 1788 brand into 850 g of water, stirring, heating to 90 ℃, dissolving, adding a small amount of evaporated water to reach a total amount of 900 g, and obtaining the PVA aqueous solution. 30 g of polyhexamethylene biguanide hydrochloride (GP2) is taken and added into 70 g of water, stirred and dissolved to prepare the guanidino polymer aqueous solution. And adding 100 g of the prepared guanidino polymer aqueous solution into 900 g of PVA aqueous solution, stirring and uniformly mixing to obtain PVA/GP2-4 composite disinfection mother liquor with the PVA concentration of 5.0% and the guanidinium polymer concentration of 3.0%.

Example 9

Adding 30 g of PVA of 1788 brand into 960 g of water, stirring, heating to 90 ℃, dissolving, adding a small amount of evaporated water to 990 g of the total amount, and thus obtaining the PVA aqueous solution. Adding 1 g of polyhexamethylene guanidine phosphate into 9 g of water, stirring and dissolving to obtain a guanidino polymer aqueous solution. Adding 10 g of prepared guanidino polymer aqueous solution into 990 g of PVA aqueous solution, stirring and uniformly mixing to obtain PVA/GP3-5 composite disinfection mother liquor with PVA concentration of 3.0% and guanidinium polymer concentration of 0.1%.

Example 10

Adding 1799 g of PVA80 g and 70 g of polyhexamethylene guanidine phosphate into 870 g of water, stirring, heating to 90 ℃, dissolving, adding a small amount of evaporated water to the total amount of 1000 g, and preparing the PVA/GP3-6 composite disinfection mother liquor with the PVA concentration of 8.0% and the guanidine salt polymer concentration of 7.0%.

Example 11

50 g of soluble Starch (Starch) and 30 g of PVA (registered trademark) of 1788 are added into 820 g of water, stirred and dissolved, the temperature is raised to 90 ℃ for further dissolution, and water is supplemented to the total amount of 900 g to obtain the Starch-PVA aqueous solution. And adding 30 g of polyhexamethylene guanidine hydrochloride into 70 g of water, stirring and dissolving to obtain a guanidino polymer aqueous solution. 100 g of the prepared guanidino polymer aqueous solution is added into 900 g of the prepared Starch-PVA aqueous solution, and the mixture is stirred and mixed evenly to obtain the Starch-PVA/GP1-1 composite disinfection mother liquor with the Starch concentration of 5.0 percent, the PVA concentration of 3.0 percent and the guanidine salt polymer concentration of 3.0 percent.

Example 12

30 g of soluble Starch (Starch) and 20 g of PVA (1799) are added into 850 g of water, stirred and dissolved, the temperature is raised to 90 ℃ for further dissolution, and water is supplemented to the total amount of 900 g to obtain the Starch-PVA aqueous solution. And adding 10 g of polyhexamethylene guanidine hydrochloride into 90 g of water, stirring and dissolving to obtain a guanidino polymer aqueous solution. 100 g of the prepared guanidino polymer aqueous solution is added into 900 g of the prepared Starch-PVA aqueous solution, and the mixture is stirred and mixed evenly to obtain the Starch-PVA/GP1-2 composite disinfection mother liquor with the Starch concentration of 3.0%, the PVA concentration of 2.0% and the guanidine salt polymer concentration of 1.0%.

Example 13

50 g of soluble Starch (Starch), 50 g of PVA with the trade name of 1799 and 80 g of polyhexamethylene guanidine hydrochloride are taken and added into 820 g of water, stirred and dissolved, the temperature is raised to 90 ℃ for further dissolution, and water is supplemented to the total amount of 1000 g to obtain the Starch-PVA/GP1-3 composite disinfection mother liquor with the Starch concentration of 5.0 percent, the PVA concentration of 5.0 percent and the guanidine salt polymer concentration of 8.0 percent.

Example 14

30 g of soluble Starch (Starch) and 20 g of PVA (1799) are added into 650 g of water, stirred and dissolved, the temperature is raised to 90 ℃ for further dissolution, and water is supplemented to the total amount of 700 g to obtain the Starch-PVA aqueous solution. And adding 10 g of polyhexamethylene guanidine hydrochloride into 90 g of water, stirring and dissolving to obtain a guanidino polymer aqueous solution. 100 g of prepared guanidino polymer aqueous solution is added into 700 g of prepared Starch-PVA aqueous solution, then 200 g of 75% alcohol is added, and the mixture is stirred and mixed uniformly to obtain Starch-PVA/GP1/Alc-1 composite disinfection mother liquor with the Starch concentration of 3.0%, the PVA concentration of 2.0%, the guanidino polymer concentration of 1.0% and the alcohol (Alc) concentration of 15%.

Example 15

30 g of soluble Starch (Starch) and 20 g of PVA (1799) are added into 450 g of water, stirred and dissolved, the temperature is raised to 90 ℃ for further dissolution, and water is supplemented to the total amount of 500 g to obtain the Starch-PVA aqueous solution. And adding 10 g of polyhexamethylene guanidine hydrochloride into 90 g of water, stirring and dissolving to obtain a guanidino polymer aqueous solution. 100 g of prepared guanidino polymer aqueous solution is added into 500 g of prepared Starch-PVA aqueous solution, then 400 g of 95% alcohol is added, and the mixture is stirred and mixed uniformly to obtain Starch-PVA/GP1/Alc-2 composite disinfection mother liquor with the Starch concentration of 3.0%, the PVA concentration of 2.0%, the guanidino polymer concentration of 1.0% and the alcohol (Alc) concentration of 38%.

Example 16

Adding 30 g of soluble Starch (Starch) into 470 g of water, stirring, dissolving, heating to 90 ℃ for further dissolving, and supplementing water to the total amount of 500 g to obtain a Starch aqueous solution. And adding 10 g of polyhexamethylene guanidine hydrochloride into 90 g of water, stirring and dissolving to obtain a guanidino polymer aqueous solution. 100 g of prepared guanidino polymer aqueous solution is added into 500 g of prepared Starch-PVA aqueous solution, 400 g of 95% alcohol is added, stirring and uniform mixing are carried out, and the Starch/GP1/Alc-1 composite disinfection mother liquor with the Starch concentration of 3.0%, the guanidine salt polymer concentration of 1.0% and the alcohol (Alc) concentration of 38% is obtained.

Example 17

100 g of polyvinylpyrrolidone (PVP) is taken and added into 800 g of water, stirred and dissolved, thus obtaining 900 g of PVP aqueous solution. And adding 30 g of polyhexamethylene guanidine hydrochloride into 70 g of water, stirring and dissolving to obtain a guanidino polymer aqueous solution. And adding 100 g of the prepared guanidino polymer aqueous solution into 900 g of PVP aqueous solution, stirring and uniformly mixing to obtain PVP/GP1-1 composite disinfection mother liquor with PVP concentration of 10% and guanidino polymer concentration of 3.0%.

Example 18

100 g of polyvinylpyrrolidone (PVP) is taken and added into 890 g of water, stirred and dissolved, thus 990 g of PVP water solution is obtained. Adding 2 g of polyhexamethylene biguanide hydrochloride into 8 g of water, stirring and dissolving to obtain a guanidino polymer aqueous solution. Adding 10 g of prepared guanidino polymer aqueous solution into 990 g of prepared PVP aqueous solution, stirring and uniformly mixing to obtain PVP/GP2-2 composite disinfection mother liquor with PVP concentration of 10% and guanidino polymer concentration of 0.2%.

Example 19

100 g of polyethylene glycol (PEG, molecular weight 8000) is added into 890 g of water, stirred and dissolved to obtain 990 g of PEG aqueous solution. Adding 3 g of polyhexamethylene biguanide hydrochloride into 7g of water, stirring and dissolving to obtain a guanidino polymer aqueous solution. Adding 10 g of prepared guanidino polymer aqueous solution into 990 g of prepared PEG aqueous solution, stirring, and uniformly mixing to obtain PEG/GP2 composite disinfection mother liquor with the PEG concentration of 10% and the guanidinium polymer concentration of 0.3%.

EXAMPLES 20 TO 26 effects of Using composite Disinfection mother liquor

Example 20

1 g of Starch/GP1-1 composite disinfection mother liquor is diluted to 500 g by adding water, and the composite disinfection solution with the guanidinium polymer content of 20ppm is obtained. The composite disinfectant is coated on an agar plate (diameter of 9cm), and then 0.1ml (10 ml) of Escherichia coli bacterial suspension is inoculated³CFU/ml); agar plates (diameter 9cm) coated with Starch/GP1-1 composite disinfectant are respectively placed under the environmental condition for 30 minutes, 1 hour, 2 hours, 5 hours and 8 hours, and then 0.1ml (10 ml) of escherichia coli bacterial suspension is inoculated³CFU/ml)。

Each agar plate inoculated with the bacterial suspension was further incubated in an incubator at 37 ℃ for 24 hours, and the growth of the bacteria was observed. The results are shown in Table 1.

Example 21

1 g of Starch-PVA/GP1-1 composite disinfection mother liquor is diluted to 1500 g by adding water, and the composite disinfection solution with the guanidinium polymer content of 20ppm is obtained. The composite disinfectant is coated on an agar plate (diameter of 9cm), and then 0.1ml (10 ml) of Escherichia coli bacterial suspension is inoculated³CFU/ml); agar plates (diameter 9cm) coated with Starch-PVA/GP1-1 composite disinfectant are respectively placed under the environmental condition for 30 minutes, 1 hour, 2 hours, 5 hours and 8 hours, and then 0.1ml (10 ml) of escherichia coli bacterial suspension is inoculated³CFU/ml)。

Example 22

1 g of Starch-PVA/GP1/Alc-1 composite disinfection mother liquor is diluted to 500 g by adding water, and the composite disinfection solution with the guanidinium polymer content of 20ppm is obtained. The composite disinfectant is coated on an agar plate (diameter of 9cm), and then 0.1ml (10 ml) of Escherichia coli bacterial suspension is inoculated³CFU/ml); agar plates (diameter 9cm) coated with Starch-PVA/GP1/Alc-1 composite disinfectant are respectively placed for 30 minutes, 1 hour, 2 hours, 5 hours and 8 hours under the environmental condition, and then 0.1ml (10 ml) of escherichia coli bacterial suspension is inoculated³CFU/ml)。

Example 23

1 g of Starch/GP1/Alc-1 composite disinfection mother liquor is diluted to 500 g by adding water, and the composite disinfection solution with the guanidine salt polymer content of 20ppm is obtained. The composite disinfectant is coated on an agar plate (diameter of 9cm), and then the large intestine is inoculated0.1ml of bacillus suspension (10)³CFU/ml); agar plates (diameter 9cm) coated with Starch/GP1/Alc-1 composite disinfectant are respectively placed under the environmental condition for 30 minutes, 1 hour, 2 hours, 5 hours and 8 hours, and then 0.1ml (10 ml) of escherichia coli bacterial suspension is inoculated³CFU/ml)。

Example 24

Taking 1 g of PVA/GP1-1 composite disinfection mother liquor, adding water to dilute the mother liquor to 100 g, and obtaining the composite disinfection solution with the guanidinium polymer content of 20 ppm. The composite disinfectant is coated on an agar plate (diameter of 9cm), and then 0.1ml (10 ml) of Escherichia coli bacterial suspension is inoculated³CFU/ml); agar plates (diameter 9cm) coated with PVA/GP1-1 composite disinfectant are respectively placed for 30 minutes, 1 hour, 2 hours, 5 hours and 8 hours under the environmental condition, and then 0.1ml (10 ml) of escherichia coli bacterial suspension is inoculated³CFU/ml)。

Example 25:

taking 1 g of Starch/GP1-1 composite disinfection mother liquor, adding water to dilute the mother liquor to 1000 g, and obtaining the composite disinfection solution with 10ppm of guanidinium polymer content. The composite disinfectant is coated on an agar plate (diameter of 9cm), and then 0.1ml (10 ml) of Escherichia coli bacterial suspension is inoculated³CFU/ml); agar plates (diameter 9cm) coated with Starch/GP1-1 composite disinfectant are respectively placed under the environmental condition for 30 minutes, 1 hour, 2 hours, 5 hours and 8 hours, and then 0.1ml (10 ml) of escherichia coli bacterial suspension is inoculated³CFU/ml). Each agar plate inoculated with the bacterial suspension was further incubated in an incubator at 37 ℃ for 24 hours, and the growth of the bacteria was observed. The results are shown in Table 1.

Example 26:

taking 1 g of PVP/GP1-1 composite disinfection mother liquor, adding water to dilute the mother liquor to 100 g, and obtaining the composite disinfection solution with the guanidinium polymer content of 300 ppm. The composite disinfectant is spread on an agar plate (diameter 9cm), and then 0.1ml of Escherichia coli bacterial suspension (10 ml) is inoculated³CFU/ml); agar plates (diameter 9cm) coated with PVP/GP1-1 composite disinfectant are respectively placed for 30 minutes, 1 hour, 2 hours, 5 hours and 8 hours under the environmental condition, and then 0.1ml (10 ml) of escherichia coli bacterial suspension is inoculated³CFU/ml). Each agar plate inoculated with the bacterial suspension was further incubated in an incubator at 37 ℃ for 24 hours, and the growth of the bacteria was observed. The results are shown in Table 1.

Comparative example 1:

spreading 75% medical sterile alcohol on agar plate (diameter 9cm), inoculating 0.1ml (10 ml) of Escherichia coli bacterial suspension³CFU/ml); the agar plates (diameter 9cm) coated with 75% alcohol disinfectant were allowed to stand under ambient conditions for 30 minutes, 1 hour, 2 hours, 5 hours, and 8 hours, respectively, and then inoculated with 0.1ml (10 ml) of Escherichia coli suspension³CFU/ml). Each agar plate inoculated with the bacterial suspension was further incubated in an incubator at 37 ℃ for 24 hours, and the growth of the bacteria was observed. The results are shown in Table 1.

Comparative example 2:

preparing disinfectant with alcohol concentration of 58% and polyhexamethylene guanidine hydrochloride concentration of 0.52%, coating on agar plate (diameter of 9cm), inoculating Escherichia coli suspension 0.1ml (10 cm)³CFU/ml); the agar plates (diameter 9cm) coated with the disinfectant are respectively placed under the environmental condition for 30 minutes, 1 hour, 2 hours, 5 hours and 8 hours, and then 0.1ml (10 ml) of Escherichia coli bacterial suspension is inoculated³CFU/ml). Each agar plate inoculated with the bacterial suspension was further incubated in an incubator at 37 ℃ for 24 hours, and the growth of the bacteria was observed. The results are shown in Table 1.

Comparative example 3:

preparing disinfectant with alcohol concentration of 58% and polyhexamethylene guanidine hydrochloride concentration of 20ppm, coating on agar plate (diameter of 9cm), inoculating Escherichia coli suspension 0.1ml (10 ml)³CFU/ml); the agar plates (diameter 9cm) coated with the disinfectant are respectively placed under the environmental condition for 30 minutes, 1 hour, 2 hours, 5 hours and 8 hours, and then 0.1ml (10 ml) of Escherichia coli bacterial suspension is inoculated³CFU/ml). Each agar plate inoculated with the bacterial suspension was further incubated in an incubator at 37 ℃ for 24 hours, and the growth of the bacteria was observed. KnotThe results are shown in Table 1.

Comparative example 4:

20ppm polyhexamethylene guanidine hydrochloride disinfectant was applied to an agar plate (diameter 9cm), and then 0.1ml (10 ml) of an Escherichia coli bacterial suspension was inoculated³CFU/ml); in addition, 20ppm polyhexamethylene guanidine hydrochloride disinfectant coated agar plate (diameter 9cm) is placed under the environment condition for 30 minutes, 1 hour, 2 hours, 5 hours and 8 hours, and then 0.1ml (10 ml) of Escherichia coli bacterial suspension is inoculated³CFU/ml). Each agar plate inoculated with the bacterial suspension was further incubated in an incubator at 37 ℃ for 24 hours, and the growth of the bacteria was observed. The results are shown in Table 1.

Comparative example 5:

100ppm polyhexamethylene guanidine hydrochloride disinfectant was applied to an agar plate (diameter 9cm), and then 0.1ml (10 ml) of an Escherichia coli bacterial suspension was inoculated³CFU/ml); in addition, 100ppm polyhexamethylene guanidine hydrochloride disinfectant coated agar plate (diameter 9cm) is placed under the environment condition for 30 minutes, 1 hour, 2 hours, 5 hours and 8 hours, and then 0.1ml (10 ml) of Escherichia coli bacterial suspension is inoculated³CFU/ml). Each agar plate inoculated with the bacterial suspension was further incubated in an incubator at 37 ℃ for 24 hours, and the growth of the bacteria was observed. The results are shown in Table 1.

Blank example:

blank agar plates (diameter 9cm) without any treatment were incubated under ambient conditions for 0 min, 1 h, 2 h, 5 h, 8 h, respectively, before inoculation with 0.1ml E.coli suspension (10)³CFU/ml). The agar plate after inoculation with the bacterial suspension was further incubated at 37 ℃ for 24 hours, and the growth of the bacteria was observed. The results are shown in Table 1.

TABLE 1 comparison of disinfection longevity of composite disinfectants

+++: no bacteria grow; ++: no or little bacterial growth; +: more bacteria grow; -: normal growth of bacteria (similar to blank case)

The following examples, on glass surfaces, the efficacy of the formulated disinfectant on escherichia coli 8099, as well as the durability of the disinfectant coating, were tested according to the disinfection specifications (version 2017). The uncoated glass surface was used as a reference.

The disinfectant solution used in the test, for example a PVA/GP1-1 composite disinfectant, had a guanidinium polymer concentration of 0.01% (100 ppm).

The glass surface is treated and dried according to the disinfection specifications, and then treated with the above-mentioned disinfection solution and dried. The uncoated glass surface was used as a reference.

Mu.l of the bacterial suspension (1 × 10)⁶cfu/ml) were pipetted onto the sample surface and spread over the entire sample surface, dried in the shade at room temperature and ready for testing.

Further, the dried sample treated with the above disinfectant was left at room temperature for 1 hour, 2 hours, 5 hours, and 8 hours, and then 10. mu.l of the bacterial suspension (1 × 10) was pipetted therein⁶cfu/ml) onto the surface of the sample piece and spread over the entire surface of the sample, dried naturally in the shade at room temperature, and ready for testing. The glass surface without the applied disinfecting liquid was taken as a reference.

In the tests, it was found that the disinfectant treated surfaces significantly reduced the concentration of e.coli bacteria on the glass surface, and the results are shown in table 2.

TABLE 2 number of colonies on the surface of the disinfectant-treated glass and the untreated glass

The embodiments described above are described to facilitate an understanding and use of the invention by those skilled in the art. It will be readily apparent to those skilled in the art that various modifications to these embodiments may be made, and the generic principles described herein may be applied to other embodiments without the use of the inventive faculty. Therefore, the present invention is not limited to the above embodiments, and those skilled in the art should make improvements and modifications within the scope of the present invention based on the disclosure of the present invention.

Claims

1. The safe and long-acting disinfection mother liquor is characterized by comprising a disinfectant, a water-soluble polymer and water, wherein the disinfectant is one or more selected from guanidine salt polymers, the lower limit of the dosage of the disinfectant is less than 30% by weight, the lower limit of the dosage of the water-soluble polymer is greater than the dosage of the disinfectant, the upper limit of the dosage of the water-soluble polymer is lower than the solubility of the water-soluble polymer in the water, and the balance of the disinfectant is the water.

2. A safe and long-acting disinfection mother liquor as claimed in claim 1, wherein the safe and long-acting disinfection mother liquor further comprises alcohol, and the concentration of the alcohol is 10-50% by weight, preferably 15-40% by weight.

3. A safe and long-lasting disinfection mother liquor according to claim 1 or 2, characterized in that the guanidinium polymer is selected from the group consisting of hydrochloride, phosphate, sulfate, nitrate or carbonate of one or several of the following:

4. A safe and long-lasting antiseptic mother liquor according to claim 1 or 2 wherein the water-soluble polymer is selected from one or more of the following: water-soluble starch, gum arabic, starch derivatives, carboxymethyl cellulose, polyvinylpyrrolidone, polyvinyl alcohol, polyacrylamide or polyethylene glycol;

preferably water-soluble starch, gum arabic, starch derivatives or carboxymethylcellulose.

5. A safe, long-lasting antiseptic mother liquor according to claim 1, characterized by being selected from one of the following compositions:

6. A safe, long-lasting antiseptic mother liquor according to claim 2, characterized by being selected from one of the following compositions:

1) the disinfectant comprises a disinfectant, a water-soluble polymer, alcohol and water, wherein the disinfectant is polyhexamethylene guanidine hydrochloride or polyhexamethylene biguanide hydrochloride, the water-soluble polymer is a mixture of polyvinyl alcohol and soluble starch, the concentration of the polyvinyl alcohol is 1-5%, the concentration of the soluble starch is 1-5%, the concentration of the disinfectant is 0.1-3.0%, and the concentration of the alcohol is 15-38%;

2) the disinfectant comprises a disinfectant, a water-soluble polymer, alcohol and water, wherein the disinfectant is polyhexamethylene guanidine hydrochloride or polyhexamethylene biguanide hydrochloride, the water-soluble polymer is soluble starch, the concentration of the soluble starch is 1-5%, the concentration of the disinfectant is 0.1-5%, and the concentration of the alcohol is 15-38%.

7. Use of a safe and long-lasting disinfectant mother liquor according to claim 1, wherein said safe and long-lasting disinfectant mother liquor is diluted to prepare a disinfectant.

8. The use of the safe and long-acting disinfectant mother liquor as claimed in claim 7, wherein the safe and long-acting disinfectant mother liquor is diluted with water to form a safe and long-acting disinfectant solution, wherein the concentration of the disinfectant guanidine salt polymer is above 10 ppm; further preferably, the concentration of disinfectant guanidine salt polymer is 10-5000 ppm;

even more preferably, the concentration of disinfectant guanidinium polymer is from 10 to 500 ppm;

still further preferably, the concentration of disinfectant guanidinium polymer is from 10 to 90 ppm.

9. The use of the safe and long-acting disinfectant solution according to claim 1, wherein the safe and long-acting disinfectant mother solution is diluted with water to form the safe and long-acting disinfectant solution, and the safe and long-acting disinfectant solution is coated or sprayed on the surface of an object to maintain long-acting and excellent disinfection performance of the surface of the object.

10. A safe and long-acting disinfectant, which is prepared by diluting the safe and long-acting disinfectant mother liquor of claim 1 or 2 with water, wherein the concentration of the antibacterial agent guanidine salt polymer in the safe and long-acting disinfectant is more than 10 ppm.