CN111771671A

CN111771671A - Tissue culture seedling transplanting matrix

Info

Publication number: CN111771671A
Application number: CN202010576865.8A
Authority: CN
Inventors: 许亮亮; 赵金华; 马盼; 左振朋; 陈雪; 门洪文; 冯锡鸿
Original assignee: Shandong Xinxile Biotechnology Co ltd
Current assignee: Shandong Xinxile Biotechnology Co ltd
Priority date: 2020-06-23
Filing date: 2020-06-23
Publication date: 2020-10-16

Abstract

The invention provides a tissue culture seedling transplanting substrate, particularly relates to a substrate for transplanting tissue culture seedlings from aseptic bottles to the outside of the bottles to harden the seedlings, and belongs to the technical field of cash crop production. The main raw materials of the invention are organic fermentation product, northeast grass carbon, moss peat and perlite, and the organic fermentation product is fermented at high temperature by high-temperature actinomycetes. Different from steam high-temperature sterilization in the prior art, the substrate treated by high-temperature actinomycetes high-temperature fermentation is 'live', the high-temperature actinomycetes is a single dominant strain, the propagation of other mixed bacteria is obviously inhibited, the survival rate of plants is effectively improved, and simultaneously, the rooting of seedlings is ensured.

Description

Tissue culture seedling transplanting matrix

Technical Field

The invention relates to a tissue culture seedling transplanting substrate, in particular to a substrate for transplanting tissue culture seedlings from a sterile bottle to the outside of the bottle and hardening the seedlings, and belongs to the technical field of cash crop production.

Background

The transplanting matrix appears in the tissue culture seedlings at the earliest time, the seedlings in sterile bottles are transplanted in the hole trays and serve as a medium for the tissue culture seedlings to excessively reach the natural environment, and the self-growing environment is greatly different before and after the tissue culture seedlings are transplanted, particularly the soil aspect, and the transplanting matrix is particularly expressed in the aspects of soil fertility, water retention, microbial diseases and the like.

Disclosure of Invention

Aiming at the problems in the prior art, the invention provides a tissue culture seedling transplanting substrate, aiming at the transition period of tissue culture seedling transplanting and hardening.

The tissue culture seedling transplanting and hardening matrix comprises an organic fermentation product, and the specific preparation method of the organic fermentation product comprises the following steps:

15-20 parts of sheep manure, 15-20 parts of cow manure, 5-25 parts of agaricus bisporus mushroom dregs, 5-25 parts of needle mushroom dregs, 10-40 parts of crop straws and 0.8-1.5% of a fermentation microbial inoculum, and the organic fermentation product is obtained after uniform mixing, fermentation and aging.

Wherein the fermentation bacteria agent is a high-temperature fermentation bacteria agent, in particular to high-temperature actinomycetes.

Wherein, the specific steps of fermentation and aging are as follows: making the fermentation raw material into strips, wherein the bottom width is 2 meters, the height is 1.5 meters, the length of the strips is not less than 5 meters, the fermentation temperature is above 75 ℃, the first pile turning is carried out, the pile turning is carried out once in 3-5 days, the temperature of a position 30cm below the surface of a pile body is maintained to be not less than 55 ℃, and the large pile is piled up after 30 days and aged for 20 days.

Wherein, the organic waste fermentation product is cooled to below 40 ℃.

The specific formula of the tissue culture seedling transplanting matrix is as follows: 20-30 parts of organic fermentation product, 20-30 parts of northeast grass carbon, 10-50 parts of moss peat and 10-20 parts of perlite.

Tissue culture seedling hardening matrix in the prior art is mostly sterilized by high-temperature steam, the aseptic degree is high, the matrix cost is high, and meanwhile, the aseptic matrix is favorable for breeding harmful microorganisms. The biological matrix provided by the invention is 'living': the dominant flora of the fermentation product obtained after the fermentation of the high-temperature actinomycetes is single, and the method has the obvious effect of inhibiting other mixed bacteria from invading the tissue culture seedling when the tissue culture seedling is hardened, and provides a natural barrier for the tissue culture seedling to gradually adapt to the external environment. The fermentation product is rich in a large amount of nutrient substances and provides rich nutrients for the growth of tissue culture seedlings.

The invention has the beneficial effects

(1) The survival rate of the tissue culture seedling in transplanting and hardening is improved and is used as a transition substrate in natural environment soil for transplanting the tissue culture seedling, and the transplanting substrate provided by the invention can be used for promoting the growth condition of a root system of the tissue culture seedling to the fastest extent and increasing the stress resistance of the tissue culture seedling;

(2) low cost, and adopts organic waste (mushroom dregs and excrement) of agriculture and animal husbandry.

Detailed Description

Transplanting and hardening the strawberry tissue culture seedling substrate: t1 steam sterilizing northeast grass carbon; t2 steam inactivation of moss peat; 20 parts of T3 organic fermentation product, 20 parts of northeast grass carbon, 50 parts of moss peat and 10 parts of perlite; CK perlite.

Selecting the Octopus vulgaris strawberries, 3-5 cm high strawberry bottle seedlings, 3-5 roots and equal root length, counting 120 bottles, washing off agar carriers, and planting in a substrate plug tray, wherein each 30 plants are treated.

Rooting powder (concentration 0.1 mg/L) is used in combination for T1, T2, T3 and CK.

The humidity in the incubator is kept between 80% and 90%, and other conditions are the same.

Investigation of survival rate and average root length of each strawberry after 10 days

Claims

1. A tissue culture seedling transplanting and hardening matrix is characterized by comprising organic fermentation products;

the specific preparation method of the organic fermentation product comprises the following steps: 15-20 parts of sheep manure, 15-20 parts of cow manure, 5-25 parts of agaricus bisporus mushroom dregs, 5-25 parts of needle mushroom dregs, 10-40 parts of crop straws and 0.8-1.5% of a fermentation microbial inoculum, and the organic fermentation product is obtained after uniform mixing, fermentation and aging.

2. The substrate according to claim 1, wherein the fermentation inoculum is a thermophilic fermentation inoculum, in particular a thermophilic actinomycete.

3. The substrate of claim 1, wherein the fermentation and aging steps are as follows: making the fermentation raw material into strips, wherein the bottom width is 2 meters, the height is 1.5 meters, the length of the strips is not less than 5 meters, the fermentation temperature is above 75 ℃, the first pile turning is carried out, the pile turning is carried out once in 3-5 days, the temperature of a position 30cm below the surface of a pile body is maintained to be not less than 55 ℃, and the large pile is piled up after 30 days and aged for 20 days.

4. The matrix of claim 1, wherein the specific formulation of the matrix for transplanting tissue culture seedlings is as follows: 20-30 parts of organic fermentation product, 20-30 parts of northeast grass carbon, 10-50 parts of moss peat and 10-20 parts of perlite.