CN111758822A - Traditional Chinese medicine compound preparation for improving intestinal microecological system and compound ganoderma lucidum tea bag - Google Patents
Traditional Chinese medicine compound preparation for improving intestinal microecological system and compound ganoderma lucidum tea bag Download PDFInfo
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Abstract
The invention provides a traditional Chinese medicine compound preparation for improving intestinal microecological system and a compound ganoderma lucidum tea bag, belongs to the technical field of compound traditional Chinese medicine health-care tea, relates to tea bags, and particularly relates to a compound ganoderma lucidum tea bag, wherein the compound ganoderma lucidum tea bag improves the intestinal microecological system by increasing the amount of intestinal short-chain fatty acids, and comprises the following components in parts by weight: 1-8 parts of ganoderma lucidum fruiting body extract dry powder, 2-10 parts of ganoderma lucidum wall-broken spore powder, 4-12 parts of black gastrodia tuber fine powder, 4-12 parts of morchella fruiting body dry powder and 80 parts of red date-medlar dry powder, wherein the ratio of red dates to medlar is 3: 1.
Description
Technical Field
The invention belongs to the field of tea bag beverages, and particularly relates to a method for preparing a compound ganoderma lucidum tea bag.
Background
Ganoderma lucidum is a traditional rare Chinese medicine for nourishing and strengthening body, strengthening body resistance and consolidating constitution, and is a rare dual-purpose fungus used as medicine and food in medical treasury of China. The systematic research of the components and the effects of the ganoderma lucidum in modern medicine further proves the peculiar medicinal function and the health care value of the ganoderma lucidum. In recent years, a large number of pharmacology and clinical tests prove that the most obvious drug effect component of ganoderma lucidum is polysaccharide, which can promote the synthesis of protein DNA and RNA of a body, promote the division and propagation of cells, stimulate the nonspecific resistance and the immune specific reactivity of a human body, inhibit the generation of tumor physiological active compounds, strengthen the immune system of the human body and improve the hematopoietic function.
Gastrodia elata is a high-grade Chinese medicinal material in China, is used for treating various diseases such as depression, epilepsy (epilepsy in sheep) and various inflammations in many countries, and has the effects of promoting intelligence, relieving pain and tranquilizing. Up to now, 81 active ingredients including phenols, polysaccharides, organic acids, sterols, etc. have been isolated from gastrodia elata. In recent years, the brain protection effect of the gastrodia elata is fully proved, and the gastrodia elata and the effective substances thereof have great breakthrough in preventing and treating brain functional diseases.
The morchella esculenta has high nutritive value, is rich in proteins, vitamins, trace elements and the like, and also contains functional active ingredients such as polysaccharide, polyphenol and the like. Research shows that the morchella has biological activities of resisting oxidation, resisting tumors, protecting livers, improving immunocompetence and the like. Polysaccharides are main active ingredients of morchella esculenta, can effectively promote the growth and development of immune organs and the proliferation of immune cells of animals, simultaneously improve the quality of main immune organs such as spleen, bursa of fabricius, thymus and mesenteric lymph node of animals, and the like, can antagonize the atrophy of immune organs caused by immunosuppressant, improve the functions of various immune cells, and play an important immune enhancement function through the mechanisms.
In recent years, teabags containing ganoderma lucidum fruiting body powder as a main component have become a research hotspot, are generally used for enhancing immunity, improving insomnia, inhibiting tumors and other diseases, and reports that active ingredients such as ganoderma lucidum are used for improving functional constipation are rarely seen.
Constipation refers to difficult or labored defecation, unsmooth defecation, decreased defecation frequency, dry and hard feces, small quantity and the like, and is the most common and very complex digestive tract symptom in clinic. Modern medicine considers that constipation is caused by lack of defecation power, insufficient stimulation on intestinal tracts, reduced sensitivity of intestinal mucosa and slow movement due to the fact that contents in the intestinal tracts are pushed forward and blocked by psychoneural factors; the main reason is that the stimulation intensity of the intestinal tract is insufficient and the defecation reflex cannot be generated due to the fact that the stretching and contraction strength of the intestinal muscle is reduced and the sensors are slow.
In recent years, studies have been conducted to find that the onset of chronic functional constipation is closely related to gastrointestinal hormones. SP is a non-cholinergic excitatory neurotransmitter, can promote the contraction of gastrointestinal smooth muscles and the secretion of gastrointestinal fluids, and has an important function of maintaining the normal digestive tract motor function. Plasma somatostatin SS inhibits smooth muscle contraction, inhibition of gastrin and secretion of pepsin. NO is an inhibitory neurotransmitter secreted by the digestive tract, and can significantly reduce gastrointestinal motility and significantly reduce gastrointestinal excitability, thereby causing severe constipation. Thus, by modulating SP, SS and NO levels, restoration of normal physiological and conductive functions of the gastrointestinal tract may be promoted.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides a traditional Chinese medicine compound preparation and a compound ganoderma lucidum tea bag for improving intestinal microecological systems, which are composed of ganoderma lucidum, gastrodia elata, morchella and red date-medlar, and the intestinal microecological environment is improved by increasing the quantity of intestinal short-chain fatty acids, so that the traditional Chinese medicine compound preparation and the compound ganoderma lucidum tea bag are expected to become a tea drink for improving functional constipation in the future.
Therefore, the invention adopts the following technical scheme:
a Chinese medicinal compound preparation for improving intestinal microecological system comprises Ganoderma fruiting body extract dry powder, Ganoderma wall-broken spore powder, rhizoma Gastrodiae powder and Morchella esculenta fruiting body dry powder.
Preferably, the ganoderma lucidum spore powder comprises ganoderma lucidum fruiting body extract dry powder, ganoderma lucidum wall-broken spore powder, gastrodia elata glaucescens fine powder and morchella esculenta fruiting body dry powder.
Specifically, the formula of the traditional Chinese medicine compound preparation is as follows: 1-8 parts of ganoderma lucidum fruiting body extract dry powder, 2-10 parts of ganoderma lucidum wall-broken spore powder, 4-12 parts of gastrodia elata fine powder and 4-12 parts of morchella fruiting body dry powder.
Most preferably, the formula of the traditional Chinese medicine compound preparation is as follows: 2 parts of dry powder of ganoderma lucidum fruiting body extract, 4 parts of ganoderma lucidum wall-broken spore powder, 5 parts of fine powder of black red gastrodia tuber, 5 parts of dry powder of morchella esculenta fruiting body and 40 parts of dry powder of red date and medlar
The Chinese medicinal compound preparation can be taken by tea bag.
The invention also provides a compound ganoderma lucidum tea bag for improving the intestinal microecological system, which improves the intestinal microecological system by increasing the quantity of the intestinal short-chain fatty acids, and comprises the following components in parts by weight:
1-8 parts of ganoderma lucidum fruiting body extract dry powder, 2-10 parts of ganoderma lucidum wall-broken spore powder, 4-12 parts of gastrodia elata fine powder and 4-12 parts of morchella fruiting body dry powder.
In addition, the coating also comprises the following components in parts by weight:
80 parts of red date-medlar dry powder, and the ratio of red date to medlar is 3: 1.
Preferably, the composition comprises the following components in parts by weight:
2-6 parts of ganoderma lucidum fruiting body extract dry powder, 4-8 parts of ganoderma lucidum wall-broken spore powder, 6-8 parts of black gastrodia tuber fine powder, 6-8 parts of morchella sporophore dry powder and 80 parts of red date-medlar dry powder.
Most preferably, the composition comprises the following components in parts by weight: 2 parts of ganoderma lucidum fruiting body extract dry powder, 4 parts of ganoderma lucidum wall-broken spore powder, 5 parts of black gastrodia tuber fine powder, 5 parts of morchella fruiting body dry powder and 40 parts of red date-medlar dry powder.
The preparation method of the compound ganoderma lucidum tea bag for improving intestinal microecological system comprises the following steps:
(1) extracting Ganoderma lucidum powder with hot water, centrifuging the extract, collecting supernatant, drying, and pulverizing into solid dry powder as Ganoderma lucidum fruiting body extract dry powder;
(2) the wall-broken spore powder of Ganoderma lucidum, the fine powder of rhizoma Gastrodiae and the dry powder of Morchella esculenta sporophore are obtained by drying and crushing non-wall-broken spore powder of Ganoderma lucidum, tuber of rhizoma Gastrodiae and Morchella esculenta sporophore respectively;
(3) mixing the above materials, oven drying, sterilizing, and packaging into sterile special filter paper bag for teabag.
Finally, the invention also provides a tea for improving functional constipation, which is prepared from the following raw materials in parts by weight, 1-8 parts of ganoderma lucidum fruiting body extract dry powder, 2-10 parts of ganoderma lucidum wall-broken spore powder, 4-12 parts of black gastrodia tuber fine powder, 4-12 parts of morchella fruiting body dry powder and 80 parts of red date-medlar dry powder, wherein the ratio of red dates to medlar is 3: 1.
The invention has the following effects:
1. can directly stimulate the intestinal tract to produce more short-chain fatty acids and indirectly improve the microecological level of the intestinal tract.
2. Can increase the number of leukocytes and lymphocytes in peripheral blood, and has the function of enhancing immunity.
3. As the tea for improving functional constipation, the pure natural Chinese medicinal materials are selected, the tea has no toxic or side effect, can be continuously brewed for 2 to 4 times, is seasoned by red dates and medlar in a compounding way, has good taste, and simultaneously promotes blood circulation and beautifies.
4. The effect of the compound ganoderma lucidum tea bag on relieving constipation is probably related to reducing inhibitory neurotransmitter and promoting excitatory neurotransmitter, and the constipation-related symptoms are relieved by improving intestinal peristalsis of constipation mice.
Detailed Description
The present invention will be further described in the following examples, but the scope of the present invention is not limited to these examples, and any modifications or other embodiments obtained by a person of ordinary skill in the art without departing from the inventive concept are included in the scope of the present invention.
The inventive concept of the present invention and the technical terms involved are described as follows:
in the process of researching the immunity enhancement of the ganoderma lucidum bagged tea drink, the inventor finds that the mixture of the ganoderma lucidum fruiting body extract dry powder, the ganoderma lucidum wall-broken spore powder, the black gastrodia tuber fine powder and the morchella fruiting body dry powder can stimulate the intestinal tract to generate more short-chain fatty acids by accident. One of the main ways that intestinal flora affects the body is to produce short chain fatty acids, SCFAs, which are end products of dietary fiber fermented by intestinal flora, i.e. free fatty acids with 1-6 carbon atoms dissolved in water.
The effect of the compound ganoderma lucidum tea bag on relieving constipation is probably related to reducing inhibitory neurotransmitter and promoting excitatory neurotransmitter, and the constipation-related symptoms are relieved by improving intestinal peristalsis of constipation mice. The method of the invention is expected to replace the existing means of treating functional constipation by medicaments, overcomes a plurality of side effects of medicament treatment and has the potential of being a compound preparation with the efficacy of improving functional constipation.
The ganoderma lucidum fruiting body extract dry powder is prepared by the following method:
1) selecting fresh red ganoderma lucidum which is reddish brown, has oily luster on the surface, does not have insect damage and does not have mildew, and cleaning;
2) removing stem, slicing into 2-3mm thick pieces with traditional Chinese medicine slicer, drying in 60-75 deg.C oven for 10-15 hr until the water content of Ganoderma lucidum fruiting body is less than or equal to eight percent;
3) crushing Ganoderma lucidum slices with a plant crusher, and sieving with a 100-mesh sieve;
4) placing Ganoderma lucidum powder into a glass double-layer reaction kettle, adding water to constant volume, stirring at 90 deg.C under 200rpm, leaching with hot water for 6-8 hr, standing overnight for fully soaking, and extracting Ganoderma lucidum fruiting body effective component;
5) centrifuging the Ganoderma lucidum water extractive solution, collecting supernatant, heating and concentrating the obtained precipitate-free supernatant for 24 hr to a certain volume, concentrating and drying with vacuum freeze dryer to obtain solid dry powder to obtain Ganoderma lucidum fruiting body extract with yield of about 6.21%;
the ganoderma lucidum wall-broken spore powder is prepared by the following method:
1) taking mature non-wall-broken spore powder (the non-wall-broken red ganoderma spore powder is light brown, relatively dry, extremely light in texture, greasy in hand and free of special smell), removing impurities, and drying the selected non-wall-broken spore powder at 50-80 deg.C for 6-12 hr;
2) crushing the dried non-wall-broken spore powder, pulverizing to particle size below 100 μm with ultramicro pulverizer, breaking wall rate above 95%, and drying to obtain wall-broken Ganoderma spore powder (wall-broken Ganoderma spore powder is dark brown, has wet feeling, occasionally caking, and has slight smell and bitter taste).
The fine powder of the gastrodia elata blume is prepared by the following method:
1) selecting good-quality, insect-free and mildew-free tuber of Gastrodia elata Blume, cleaning to remove soil on the surface;
2) drying the cleaned tuber of the black red gastrodia elata in an oven, and keeping the tuber of the black red gastrodia elata for later use when the water content of the tuber of the black red gastrodia elata is not changed any more;
3) grinding the dried tuber of tall gastrodia tuber of black red into powder by a grinder, and sieving the powder by a sample sieve of 100 meshes to obtain fine powder of tall gastrodia tuber of black red;
the morchella sporocarp dry powder is prepared by the following method:
1) cleaning silt on the surface of the collected artificially-cultivated black morchella sporocarp, and drying in an oven;
2) crushing dried morchella esculenta with a plant crusher, and sieving with a 100-mesh sieve to obtain dried morchella esculenta sporophore powder;
the red date dry powder is prepared by the following method:
1) selecting red dates with good quality, no worm bodies and no mildew, cleaning and removing kernels;
2) drying the red dates with the kernels removed in the sun, crushing the red dates by using a plant crusher, and screening the crushed red dates by using a 100-mesh sample screen to obtain red date dry powder;
the medlar dry powder is prepared by the following method:
1) selecting red wolfberry fruits with good quality and without worm bodies and mildew, and cleaning;
2) dehydrating and drying the washed red medlar, crushing the red medlar by a plant crusher, and sieving the crushed red medlar by a 100-mesh sieve to obtain medlar dry powder;
example 1
The formula and the production process of the compound ganoderma lucidum tea bag comprise the following steps: taking 0.2 g of ganoderma lucidum fruiting body extract dry powder, 0.25 g of ganoderma lucidum wall-broken spore powder, 0.3 g of black gastrodia tuber fine powder, 0.3 g of morchella fruiting body dry powder and 2 g of red date-medlar (ratio 3:1) dry powder. Mixing the above materials, oven drying, sterilizing, packaging, and making into teabag for administration by soaking in boiling water. 10 volunteers were invited to make tea for drinking, and the taste evaluation was identified as good.
Example 2
The formula and the production process of the compound ganoderma lucidum tea bag comprise the following steps: taking 0.1 g of ganoderma lucidum fruiting body extract dry powder, 0.2 g of ganoderma lucidum wall-broken spore powder, 0.25 g of gastrodia elata fine powder, 0.25 g of morchella fruiting body dry powder and 2 g of red date-medlar (ratio 3:1) dry powder. Mixing the above materials, oven drying, sterilizing, packaging, and making into teabag for administration by soaking in boiling water. 10 volunteers were invited to make tea for drinking, and the taste evaluation was identified as good.
Example 3
The formula and the production process of the compound ganoderma lucidum tea bag comprise the following steps: taking 0.05 g of ganoderma lucidum fruiting body extract dry powder, 0.15 g of ganoderma lucidum wall-broken spore powder, 0.2 g of gastrodia elata fine powder, 0.2 g of morchella fruiting body dry powder and 2 g of red date-medlar (ratio 3:1) dry powder. Mixing the above materials, oven drying, sterilizing, packaging, and making into teabag for administration by soaking in boiling water. 10 volunteers were invited to make tea for drinking, and the taste evaluation was identified as good.
Example 4
The formula and the production process of the compound ganoderma lucidum tea bag comprise the following steps: taking 0.025 g of ganoderma lucidum fruiting body extract dry powder, 0.1 g of ganoderma lucidum wall-broken spore powder, 0.15 g of gastrodia elata fine powder, 0.15 g of morchella fruiting body dry powder and 2 g of red date-medlar (ratio 3:1) dry powder. Mixing the above materials, oven drying, sterilizing, packaging, and making into teabag for administration by soaking in boiling water. 10 volunteers were invited to make tea for drinking, and the taste evaluation was identified as good.
Example 5
The formula and the production process of the compound ganoderma lucidum tea bag comprise the following steps: taking 0.025 g of ganoderma lucidum fruiting body extract dry powder, 0.05 g of ganoderma lucidum wall-broken spore powder, 0.1 g of black gastrodia tuber fine powder, 0.1 g of morchella fruiting body dry powder and 2 g of red date-medlar (ratio 3:1) dry powder. Mixing the above materials, oven drying, sterilizing, packaging, and making into teabag for administration by soaking in boiling water. 10 volunteers were invited to make tea for drinking, and the taste evaluation was identified as good.
Example 6
The formula and the production process of the compound ganoderma lucidum tea bag comprise the following steps: taking 0.8 g of ganoderma lucidum fruiting body extract dry powder, 1 g of ganoderma lucidum wall-broken spore powder, 1.2 g of gastrodia elata fine powder, 1.2 g of morchella fruiting body dry powder and 8 g of red date-medlar (ratio 3:1) dry powder. Mixing the above materials, oven drying, sterilizing, packaging, and making into teabag for administration by soaking in boiling water. 10 volunteers were invited to make tea for drinking, and the taste evaluation was identified as good.
Example 7
The formula and the production process of the tea for improving functional constipation comprise the following steps: taking 0.1 g of ganoderma lucidum fruiting body extract dry powder, 0.2 g of ganoderma lucidum wall-broken spore powder, 0.25 g of gastrodia elata fine powder, 0.25 g of morchella fruiting body dry powder and 2 g of red date-medlar (ratio 3:1) dry powder. Mixing the above materials, oven drying, sterilizing, and packaging to obtain weight reducing tea, which can be administered by soaking in boiling water for 2-4 times every day. 10 volunteers were invited to make tea for drinking, and the taste evaluation was identified as good.
Example 8
The formula and the production process of the compound preparation are as follows: taking 0.1 g of ganoderma lucidum fruiting body extract dry powder, 0.2 g of ganoderma lucidum wall-broken spore powder, 0.25 g of gastrodia elata fine powder, 0.25 g of morchella fruiting body dry powder and 2 g of red date-medlar (ratio 3:1) dry powder. Mixing the above materials, oven drying, and sterilizing to obtain the compound preparation.
The compound ganoderma lucidum teabag prepared by the invention can be used for promoting the proliferation of peripheral blood leukocytes and lymphocytes and regulating the gastrointestinal hormone level, and the effect is shown in the following experiment.
The biological activity of the compound ganoderma lucidum tea bag is expressed by the influence degree of the compound ganoderma lucidum tea bag on the quantity of white blood cells and lymphocytes in peripheral blood of a mouse and the contents of SP, SS and NO in serum of the mouse.
(1) Experimental methods
Animal experiments:
90 SPF BALB/c mice (8 weeks old, 18-20 g) were randomly divided into 9 groups of 10 mice each, and after being reared for one week under sterile conditions at 22 + -1 deg.C, the mice were administered by gavage for 14 days. The CT group is a blank control group, and the mice are not treated; PL group is low concentration experimental group, and is compound Ganoderma teabag with stomach-feeding amount of 50mg/Kg per day for mouse; PM group is middle concentration experiment group, and is compound Ganoderma teabag with weight of 150mg/Kg for intragastric administration of mice every day; the PH group is a high-concentration experimental group, and the mice are infused with 300mg/Kg weight of compound ganoderma lucidum tea bags every day; CTP1 group is experiment control group 1, and is prepared by intragastric administration of 150mg/Kg dry powder of Ganoderma lucidum fruiting body extract for mice per day; CTP2 group is experiment control group 2, and is prepared by intragastric administration of 150mg/Kg weight of Ganoderma lucidum wall-broken spore powder to mice every day; CTP3 group is experiment control group 3, and is prepared by intragastric administration of 150mg/Kg of rhizoma Gastrodiae fine powder per day; the CTP4 group is an experimental control group 4, and the mice are gavaged with 150mg/Kg of dry morchella sporocarp powder per day; the CTP5 group is an experimental control group 5, and the mice are intragastrically filled with 150mg/Kg of mixed dry powder of red dates and medlar every day. After 14 days, the number of leukocytes and lymphocytes in peripheral blood of the mice was measured using a fully automatic blood cell analyzer, and the results are shown in table 1.
Analysis of microbial diversity of cecal contents:
on day 15, mice were sacrificed and the cecal contents of each group of mice were collected and the bacterial 16S rDNA in the cecal contents were sequenced using a double-ended sequencing (Paired-End) method to construct a small fragment library. Followed by bioinformatics analysis such as splice filtration of DNA sequencing sequences, OTUs (operational nucleic acids units) clustering, species annotation and abundance analysis to reveal the species composition of the samples. At the family level, the relative abundance of short chain fatty acid producing bacteria is shown in table 2.
Detection of short-chain fatty acids in the large intestine:
on day 15, mice were sacrificed and the large intestine contents of each group of mice were collected and homogeneously diluted with distilled water at a ratio of 1: 6; after mixing, standing at room temperature for 30min, centrifuging at 5000r/min for 10min, taking 0.1ml of supernatant, adding 0.2% concentrated hydrochloric acid and 2-ethylbutyric acid (as internal standard, the final concentration is 1mmol/L), standing at 4 ℃ for 30min, centrifuging at 6000r/min for 10min, taking supernatant, filtering with 0.22um filter membrane, and analyzing by gas chromatography.
The chromatographic analysis was carried out by gas chromatography using a DB-FFAP column (30m X0.535 mm X1um) and FID detector. Gas phase analysis conditions: the carrier gas is helium, the flow rate of the helium is 1.0mL/min, and the split ratio is 1: 25; the detector temperature was 260 ℃ and the injection port temperature was 250 ℃. Column oven temperature program: keeping the temperature at 50 ℃ for 1 minute, then heating from 50 ℃ to 120 ℃ at a heating rate of 15 ℃/min, then heating from 120 ℃ to 170 ℃ at a heating rate of 5 ℃/min, and then heating from 170 ℃ to 240 ℃ at a heating rate of 15 ℃/min; finally, the temperature was maintained at 240 ℃ for 3.0 minutes. The sample size was 1 uL.
The standard curve was plotted using n-butyric acid, isobutyric acid, n-valeric acid, and isovaleric acid as standards, using the peak areas as the Y coordinate axis and the acid concentrations as the X coordinate axis according to the above method. The contents of butyric acid and valeric acid in the large intestine of each group of mice are shown in Table 3.
② establishment of constipation model
30 male SPF BALB/c mice (8 weeks old, 18-20 g) were allowed to eat water freely at room temperature (23. + -. 1). degree.C.for a 12h day-night cycle. After 1 week of feeding, the mice were randomly divided into 3 groups, namely a normal group (10 mice, hereinafter referred to as group A), a model group (10 mice, hereinafter referred to as group B), and a compound ganoderma lucidum tea bag treatment group (10 mice, hereinafter referred to as group C). Group B mice were gavaged 1 times daily with loperamide hydrochloride (9.6mg/kg) in the morning and evening for 2 weeks. When the mice have the symptoms of poor mental state, reduced food intake, increased water intake, weight loss, small stool grain, dryness, hardness, reduced defecation amount and the like, the molding success is indicated. The time and frequency of gavage hydrochloric acid in group C mice are the same as those in group B mice, and the gavage hydrochloric acid lasts for 2 weeks, but after the gavage hydrochloric acid is used for 1 week, the gavage is performed for 1 time every day, and the duration is 1 week. Normal group a was gavaged daily with the same volume of saline.
Index detection:
during the establishment of a constipation model of a mouse, regularly collecting excrement grains of the mouse for 4 hours every day, weighing the wet weight of the excrement grains, putting the excrement grains into an electric heating drying box, drying the excrement grains for 8 hours at 50 ℃, weighing the dry weight of the excrement grains, and recording data. After 1 week of administration, each group of mice was fasted but not water inhibited for 12h, anesthetized with 10% chloral hydrate, blood was taken from abdominal aorta, left at room temperature for 30min, centrifuged at 3000r/min for 10min to obtain mouse serum, and contents of SP, SS and NO in the serum were measured by radioimmunoassay and nitroreductase method, respectively.
(2) Results of the experiment
TABLE 1 influence of bagged tea of compound Ganoderma on the number of leukocytes and lymphocytes in peripheral blood of mice
Statistical analysis shows that the number of white blood cells and lymphocytes in peripheral blood of mice in an experimental group is obviously higher than that of a blank control group (p is less than 0.05), which indicates that the compound ganoderma lucidum teabag obtained by the invention has obvious effect on promoting the proliferation of white blood cells and lymphocytes in peripheral blood. In addition, under the same feeding amount condition, the numbers of white blood cells and lymphocytes in peripheral blood of mice in a medium-concentration experimental group are obviously higher than those in an experimental control group (p <0.05), which shows that the components of the invention have the synergistic effect in promoting the proliferation of the white blood cells and the lymphocytes in the peripheral blood.
TABLE 2 relative abundance ratios of dominant species at family level
In the above table, short chain fatty acid producing bacteriaaDescription of (1): since the short-chain fatty acid-producing bacteria are mainly distributed among 3 families of bacteria, Lachnospiraceae, ruminococcus and Erysipelotrichaceae, the proportion of the short-chain fatty acid-producing bacteria is mainly the sum of the proportions of the three families of bacteria.
TABLE 3 content of short-chain fatty acids in large intestine of each group of mice
Through statistical analysis, the contents of bacteria producing short-chain fatty acids in the caecum and butyric acid and valeric acid in the large intestine contents of the experimental group are both obviously higher than those of a blank control group (p is less than 0.05), which indicates that the compound ganoderma lucidum teabag obtained by the invention has obvious effect on promoting the proliferation of the bacteria producing short-chain fatty acids and further promoting the generation of the short-chain fatty acids in the intestinal tract. In addition, under the condition of the same feeding amount, the contents of the bacteria capable of generating short-chain fatty acid in the cecum of the mice in the medium-concentration experimental group and the contents of butyric acid and valeric acid in the large intestine are both obviously higher than those in the experimental control group (p is less than 0.05), which indicates that the invention has the function of promoting the proliferation of the bacteria capable of generating short-chain fatty acid and further promoting the generation of the short-chain fatty acid in the intestinal tract.
TABLE 4 influence of bagged tea of compound Ganoderma lucidum on the laxative action of mice (n ═ 10)
Statistics shows that the frequency of defecating, the dry weight of the defecating and the water content of excrement of mice in a group B are remarkably reduced (p is less than 0.05) compared with the mice in the group A within 4h of defecating observation; group C mice showed significant increases in stool frequency, stool dry weight, and stool water content (p <0.05) compared to group B, see table 4. The experimental result shows that the stomach-irrigation administration of the compound ganoderma lucidum tea bag has obvious function of relaxing the bowels for the constipation mice.
TABLE 5 serum SP, SS and NO content of each group of mice (n ═ 10)
Through analysis, compared with the group A, the content of SP in the serum of mice in the group B is obviously reduced, the content of SS is obviously increased, and the level of NO is obviously increased (p is less than 0.05); compared with the group B, the increase and recovery of the SP content in the serum of the mice in the group C are realized, the SS content is obviously reduced, and the NO level is obviously reduced (p is less than 0.05), which is shown in a table 5. The results suggest that the constipation relieving effect of the compound ganoderma lucidum tea bag is possibly related to the reduction of inhibitory neurotransmitter and the promotion of excitatory neurotransmitter, and the constipation related symptoms are relieved by improving the intestinal peristalsis of constipation mice.
Comparative example 1
The formula and the production process of the tea bag comprise: taking 0.1 g of ganoderma lucidum fruiting body extract dry powder, 0.2 g of ganoderma lucidum wall-broken spore powder, 0.25 g of morchella esculenta fruiting body dry powder and 2 g of red date-medlar (ratio 3:1) dry powder. Mixing the above materials, oven drying, sterilizing, and packaging.
The detection of short chain fatty acids in the large intestine is shown in Table 6.
Comparative example 2
The formula and the production process of the tea bag comprise: taking 0.1 g of ganoderma lucidum fruiting body extract dry powder, 0.2 g of ganoderma lucidum wall-broken spore powder, 0.25 g of gastrodia elata glaucens fine powder and 2 g of red date-medlar (ratio 3:1) dry powder. Mixing the above materials, oven drying, sterilizing, and packaging.
The detection of short chain fatty acids in the large intestine is shown in Table 6.
Comparative example 3
The formula and the production process of the tea bag comprise: taking 0.2 g of ganoderma wall-broken spore powder, 0.25 g of fine powder of black red gastrodia tuber, 0.25 g of morchella sporocarp dry powder and 2 g of red date-medlar (ratio 3:1) dry powder. Mixing the above materials, oven drying, sterilizing, and packaging.
The detection of short chain fatty acids in the large intestine is shown in Table 6.
TABLE 6 relative abundance ratios of dominant species at family level
Further demonstrated by a comparative example, in the aspect of promoting the generation of short chain fatty acid producing bacteria, the ganoderma lucidum fruiting body extract dry powder, ganoderma lucidum wall-broken spore powder, gastrodia elata fine powder and morchella fruiting body dry powder have a synergistic effect, and further mechanisms are researched.
Comparative example 4
The formula and the production process of the tea bag comprise: taking 0.1 g of ganoderma lucidum fruiting body extract dry powder, 0.2 g of ganoderma lucidum wall-broken spore powder, 0.25 g of gastrodia elata glaucens fine powder and 0.25 g of morchella fruiting body dry powder. Mixing the above materials, oven drying, sterilizing, and packaging. When 10 volunteers were invited to make tea for drinking, the general reaction was bitter and astringent, and the experience was poor.
Claims (10)
1. A compound ganoderma lucidum tea bag for improving intestinal microecosystem is characterized in that: the compound ganoderma lucidum tea bag improves intestinal microecosystem by increasing the amount of intestinal short-chain fatty acid, and comprises ganoderma lucidum fruiting body extract dry powder, ganoderma lucidum wall-broken spore powder, gastrodia elata powder and morchella sporocarp dry powder.
2. The compound ganoderma lucidum tea bag for improving intestinal microecosystem of claim 1, which is characterized in that: comprises Ganoderma lucidum fruiting body extract dry powder, Ganoderma lucidum wall-broken spore powder, rhizoma Gastrodiae Preparata fine powder and Morchella esculenta fruiting body dry powder.
3. The compound ganoderma lucidum tea bag for improving intestinal microecosystem of claim 1, which is characterized in that: the paint comprises the following components in parts by weight:
1-8 parts of ganoderma lucidum fruiting body extract dry powder, 2-10 parts of ganoderma lucidum wall-broken spore powder, 4-12 parts of gastrodia elata fine powder and 4-12 parts of morchella fruiting body dry powder.
4. The compound ganoderma lucidum tea bag for improving intestinal microecosystem of claim 1, which is characterized in that: the paint also comprises the following components in parts by weight:
80 parts of red date-medlar dry powder, and the ratio of red date to medlar is 3: 1.
5. The compound ganoderma lucidum tea bag for improving intestinal microecosystem of claim 4, wherein: the paint comprises the following components in parts by weight:
2-6 parts of ganoderma lucidum fruiting body extract dry powder, 4-8 parts of ganoderma lucidum wall-broken spore powder, 6-8 parts of black gastrodia tuber fine powder, 6-8 parts of morchella sporophore dry powder and 80 parts of red date-medlar dry powder.
6. The compound ganoderma lucidum tea bag for improving intestinal microecosystem of claim 4, wherein: the paint comprises the following components in parts by weight: 2 parts of ganoderma lucidum fruiting body extract dry powder, 4 parts of ganoderma lucidum wall-broken spore powder, 5 parts of black gastrodia tuber fine powder, 5 parts of morchella fruiting body dry powder and 40 parts of red date-medlar dry powder.
7. The compound ganoderma lucidum tea bag for improving intestinal microecosystem of claim 4, wherein: the preparation method of the compound ganoderma lucidum tea bag comprises the following steps:
(1) extracting Ganoderma lucidum powder with hot water, centrifuging the extract, collecting supernatant, drying, and pulverizing into solid dry powder as Ganoderma lucidum fruiting body extract dry powder;
(2) the wall-broken spore powder of Ganoderma lucidum, the fine powder of rhizoma Gastrodiae and the dry powder of Morchella esculenta sporophore are obtained by drying and crushing non-wall-broken spore powder of Ganoderma lucidum, tuber of rhizoma Gastrodiae and Morchella esculenta sporophore respectively;
(3) the composition ratio of any one of claims 3-6, wherein the components are mixed, dried, sterilized and packaged into sterile filter paper bags special for tea bags.
8. A traditional Chinese medicine compound preparation for improving intestinal microecological system is characterized in that: the formula of the traditional Chinese medicine compound preparation is as follows: 1-8 parts of ganoderma lucidum fruiting body extract dry powder, 2-10 parts of ganoderma lucidum wall-broken spore powder, 4-12 parts of gastrodia elata fine powder and 4-12 parts of morchella fruiting body dry powder.
9. The compound traditional Chinese medicine preparation for improving intestinal microecological system according to claim 6, wherein: the formula of the traditional Chinese medicine compound preparation is as follows: 2 parts of ganoderma lucidum fruiting body extract dry powder, 4 parts of ganoderma lucidum wall-broken spore powder, 5 parts of black gastrodia tuber fine powder, 5 parts of morchella fruiting body dry powder and 40 parts of red date-medlar dry powder.
10. A tea for improving functional constipation is characterized in that: the ganoderma lucidum spore powder is prepared from the following raw materials, by weight, 1-8 parts of ganoderma lucidum fruiting body extract dry powder, 2-10 parts of ganoderma lucidum wall-broken spore powder, 4-12 parts of black gastrodia tuber fine powder, 4-12 parts of morchella fruiting body dry powder and 80 parts of red date-Chinese wolfberry dry powder, wherein the ratio of red dates to Chinese wolfberry is 3: 1.
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