CN111727033A - Stain-proofing agent and composition for external use on skin - Google Patents

Abstract

Provided herein are an antifouling agent excellent in the effect of preventing or inhibiting skin inflammation caused by particulate air pollutants, and a skin external composition for antifouling. An antifouling agent, wherein a plant-derived polyamine-containing extract prevents or inhibits skin inflammation caused by atmospheric pollutants.

Description

Stain-proofing agent and composition for external use on skin

Technical Field

The present invention relates to an antifouling agent for alleviating skin damage caused by airborne contaminants, particularly particulate airborne contaminants, and a skin external composition containing the same.

Background

The skin is the largest tissue located at the outermost layer and is exposed to external stimuli such as ultraviolet rays, microorganisms, viruses, air pollutants, and the like. Ultraviolet rays have been studied for a long time to cause skin aging by damaging the skin. On the other hand, in recent years, cases have been increasing in which the possibility of adhesion of particulate air pollutants to the skin to participate in aging is reported (non-patent document 1). Further, it has been reported that benzopyrene contained in tobacco activates an aromatic hydrocarbon receptor present in epidermal cells, promotes induction of cytochrome P450(CYP1) which is a drug metabolizing enzyme, generates active oxygen, induces production of interleukin 8, and causes an inflammatory reaction (non-patent document 2).

In order to protect the skin from such external irritation, it is considered to prevent particulate air pollutants or the like, which are substances causing damage, from adhering to the skin and permeating therethrough, and/or to alleviate the influence of adhered substances causing irritation on the inside of the skin.

As a measure for protecting the skin as described above, a method has been proposed in the past in which a film is formed on the skin to prevent particulate air pollutants from adhering to the skin or an agent that is easily washed away is adhered to the skin, but the skin is covered with the film, and therefore the feeling of use is poor, and it is not clear whether or not the skin is effective for all substances that cause irritation.

Since particulate air pollutants are attached to and permeate the skin to apply oxidative stress to cells to cause inflammatory reaction, cell metabolism, gene damage, and the like, the mechanisms of cell damage are extremely diverse and unknown, including abnormalities in oxidation, inflammation, cell metabolism, gene expression, and the like, and thus, the number of raw materials having an anti-pollution effect in the market is currently not large.

For example, it has been found that an extract of Hippophae (Hippophae) belonging to the genus Hippophae of the family elaeageae inhibits the increase of PGE2 caused by atmospheric aerosol particles such as particulate air pollutants, thereby inhibiting the action of skin inflammation caused by the atmospheric aerosol particles (patent document 1). In addition, focusing attention on the mechanism of inducing skin inflammation in epidermal cells by contaminants permeating into the skin, the usefulness of the present inventors has been found that the cell injury mitigating action by zinc salts of amino acids and/or zinc complexes, the CYP1 excess inducing action, the COX2 excess inducing action, the inhibition of the IL8 excess inducing action, and the like are useful (patent document 2).

In addition to the above, development of a new antifouling agent has been required from the viewpoints of diversification of preparations, increase of options for composition, synergistic effects of antifouling agents against particulate air pollutants having different mechanisms of use, and the like, and from the viewpoints of safeness and high effectiveness of antifouling agents.

Polyamines are a generic term for aliphatic hydrocarbons having 2 or more primary amino groups, and 20 or more polyamines have been found as natural products commonly existing in living organisms. Typical polyamines include putrescine, spermidine, and spermine. As main physiological actions of polyamines, (1) stabilization and structural change of nucleic acids by interaction with nucleic acids, (2) acceleration of various nucleic acid synthesis systems, (3) activation of protein synthesis systems, (4) stabilization of cell membranes and enhancement of membrane permeability of substances, (5) elimination of active oxygen, and (6) acceleration of cell proliferation are known, but the effect of preventing or suppressing skin inflammation caused by particulate air pollutants has not been confirmed yet.

Documents of the prior art

Patent document

Patent document 1: japanese patent laid-open publication No. 2016-216366

Patent document 2: japanese patent laid-open publication No. 2016-88929

Non-patent document

Non-patent document 1: j Eur Acad dermotol venereol.2015dec; 29(12): 2326-32

Non-patent document 2: j Dermatol Sci.2011Apr; 62(1): 42-9

Summary of The Invention

The present invention provides an antifouling agent having an excellent effect of preventing or inhibiting skin inflammation caused by particulate air pollutants, and an external composition for skin using the antifouling agent.

Means for solving the problems

The present inventors have found that a plant-derived polyamine-containing extract has an anti-pollution effect of mitigating skin inflammation and the like caused by air pollutants, and thus have completed the present invention.

That is, the present invention is as follows.

Item 1. antifouling agent containing polyamine-containing extract of plant origin as an active ingredient.

The antifouling agent according to item 1, wherein the polyamine-containing extract derived from a plant is an extract derived from at least one material selected from the group consisting of soybean seeds, soybean germs, soybean sprouts, wheat seeds, wheat germs, wheat malt, soybean milk, and soybean dregs.

Item 3. the stain resistant agent of item 1 or 2, wherein the plant-derived polyamine-containing extract is a soybean germ-derived extract.

The antifouling agent according to any one of claims 1 to 3, wherein the polyamine comprises at least one compound selected from the group consisting of putrescine, spermidine and spermine.

Item 5 the antifouling agent according to any one of items 1 to 4, which has an effect of inhibiting the expression of at least 1 gene selected from the group consisting of interleukin-1 α (IL1 α), interleukin-8 (IL8) and cytochrome P450(CYP 1).

An external composition for skin comprising the antifouling agent according to any one of items 1 to 5.

Effects of the invention

It is expected that the use of the plant-derived polyamine-containing extract of the present invention can prevent or suppress skin inflammation caused by particulate air pollutants, and can provide an external preparation for skin aiming at the effect.

Drawings

FIG. 1 is a graph showing the results of the expression level of IL 1a in example 1, after the keratinocytes were treated with soybean extract in advance and treated with urban atmospheric dust (environmental standard substance)/CRM No.28) for 2 hours after 5 hours.

FIG. 2 is a graph showing the results of the expression level of IL8 after the keratinocytes in example 1 were treated with soybean extract in advance and treated with city atmospheric dust (environmental standard substance)/CRM No.28) for 2 hours after 5 hours.

FIG. 3 is a graph showing the results of the expression level of CYP1 after the treatment of keratinocytes with soybean extract in advance and the treatment with urban atmospheric dust (environmental standard substance)/CRM No.28) for 2 hours after 5 hours in example 1.

Detailed Description

In the present invention, the air pollutant means a harmful substance or a group of harmful substances existing in the atmosphere. Specific examples include, but are not limited to, soot, dust, automobile exhaust gas, specific substances, harmful air pollutants, Volatile Organic Compounds (VOC), and the like, which are the subject of control by the air pollution prevention act (homepage of environmental regeneration protection agency of independent administrative law; https:// www.erca.go.jp/yobo/taiki/taisa ku/01 — 01. html). More specifically, there are mentioned floating particulate matters containing various harmful substances such as PM10 and PM2.5, and the anti-staining agent and the composition for external application to skin containing the same of the present invention can prevent or inhibit skin inflammation caused by PM2.5 in particular. In the present invention, anti-contamination means an effect of preventing or inhibiting inflammatory reaction of the skin due to air pollutants. Specifically, the effect of inhibiting the gene expression of IL1 α, IL8 or CYP1 can be exhibited as a criterion, but is not particularly limited thereto.

"plant extract" in the present invention means those obtained from plants and/or plant processed products.

In the present invention, "polyamine-containing extract of plant origin" means a plant extract containing (natural) polyamines obtained from plants and/or plant processed products, a plant extract containing 3 kinds of (natural) putrescine, spermidine, spermine obtained from plants and/or plant processed products, and a plant extract containing 3 kinds of (natural) putrescine, spermidine, spermine obtained from plants and/or plant processed products. In the present invention, the "polyamine-containing extract of plant origin" is also referred to as "polyamine-containing plant extract and/or plant extract containing polyamine as an active component". The polyamine concentration in the polyamine-containing extract derived from a plant is usually 0.00001 to 100mM, preferably 0.00005 to 75mM, and more preferably 0.0001 to 50mM, in terms of M (mol/l). The content of the metal oxide is usually 0.0001 to 100% by weight, preferably 0.001 to 75% by weight, and more preferably 0.01 to 50% by weight.

It is presumed that the polyamine-containing extract of plant origin is more excellent in the anti-fouling effect than the compositions each containing chemically synthesized putrescine, spermidine, spermine because it contains natural active components other than the polyamines of putrescine, spermidine, spermine at the same time. As natural active ingredients, there may be mentioned, for example: saccharides such as monosaccharides and oligosaccharides, peptides, proteins, etc. Furthermore, since the plant-derived polyamine-containing extract is an aqueous solution, the production conditions are milder than those of the respective polyamine compounds, and an advantage of high safety is expected.

In the present invention, polyamine refers to a generic term of aliphatic hydrocarbons having 2 or more primary amino groups, and 20 or more polyamines have been found as natural products commonly existing in living bodies. Examples thereof include: 1, 3-diaminopropane, putrescine, cadaverine, bis (3-aminopropyl) amine (cardine), spermidine, homo-spermidine (homopsperamine), aminopropylcadaverine, terminine (terminine), spermine, heat-spermine, canavanine (canavaline), aminopentyl norspermine (aminopentoranpersemine), N-bis (aminopropyl) cadaverine, homo-spermine, thermophilic pentamine (caldopentamine), homothermophilic pentamine (homothermophilic pentamine), thermophilic hexamine (calhexamine), homothermophilic hexamine (homocalhexamine), and the like. Typical polyamines include putrescine, spermidine, and spermine.

The "putrescine" mentioned in the present invention is one of representative polyamines, which are general natural substances commonly existing in living organisms, and are aliphatic hydrocarbon compounds having 2 primary amino groups. "spermidine" is one of the representative polyamines, and is a general natural product existing in living bodies, and is an aliphatic hydrocarbon compound having 3 primary amino groups. "spermine" is one of the representative polyamines, and is a general natural product existing in living bodies, and is an aliphatic hydrocarbon compound having 4 primary amino groups.

The plant from which the polyamine-containing plant extract and/or the plant extract containing polyamine as an active component is obtained is not particularly limited, and examples thereof include dicotyledons, monocotyledons, herbaceous plants, arbor plants, cucurbitaceae plants, solanaceae plants, gramineae plants, cruciferae plants, leguminous plants, malvaceae plants, compositae plants, chenopodiaceae plants, leguminous plants, extracts of the plants, essences of the plants, and the like. Examples thereof include: sweet potato, tomato, cucumber, pumpkin, melon, watermelon, tobacco, Arabidopsis (Arabidopsis thaliana), sweet pepper, eggplant, bean, Araceae (aroid), spinach, carrot, strawberry, potato, rice, corn, alfalfa, wheat, barley, soybean, rape, sorghum, eucalyptus (eucalyptus), poplar (aspen), kenaf (kenaf), eucommia (Eucommiaulmoides), sugarcane, sugar beet, cassava, sago palm, Chenopodiaceae (quinoa), lily, orchid, carnation, rose, chrysanthemum, petunia, butterfly plant (torenia), snapdragon, cyclamen, star, geranium, sunflower, turf grass, cotton, needle mushroom (velvet sank), yuzu (Lyophyllum, phyllum), pine mushroom (Tricholoma matsutake), mushroom (Agaricus blazei), black tea, Banana, kiwi, natto, soybean milk, soybean essence, wheat essence, germ essence, fruit juice, bean dregs (okara), rice germ, wheat germ, barley germ, soybean germ, corn germ, milo germ, sunflower germ and the like.

The plant or processed product thereof is preferably a monocotyledon or dicotyledon, more preferably a rice plant or leguminous plant, and examples of the particularly preferred plant or processed product thereof include corn, mushroom, soybean, wheat, natto, soybean milk, soybean residue, wheat germ, soybean germ, corn germ, soybean essence, wheat essence, germ essence, and germ essence. Further preferable examples include soybean seeds, soybean germs, soybean sprouts, wheat seeds, wheat germs, wheat malt, soybean milk, and soybean dregs.

Plant tissues from which polyamine-containing plant extracts and/or plant extracts containing polyamines as active components are obtained are not particularly limited. Preferably in seed form, those that are in the process of growing. The plant in the process of growing may be obtained from all or part of the tissue. The site that can be obtained is not particularly limited, and is a whole plant, a flower bud, an ovary, a fruit, a leaf, a cotyledon, a stem, a bud, a root, a seed, a dried seed, an embryo, a germ, a root, or the like. Preferably fruit, leaf, stem, bud, seed, dried seed, embryo, particularly preferably seed, dried seed, embryo and the like.

The plant from which the polyamine-containing plant extract and/or the plant extract containing polyamine as an active component is obtained may be a processed plant. The processing method comprises recovering the extract from the plant by soaking, distilling, squeezing, ultrasonic method, supercritical fluid method, or subcritical fluid method at low temperature, room temperature, and heating conditions with water, organic solvent, or mixture of water and organic solvent. Also included are processed products obtained by further subjecting plants and extracts recovered from plants to a processing treatment such as fermentation. Examples thereof include plant essence, soybean milk, soybean dregs, flour, fermentation essence, natto and the like.

As a method for obtaining a polyamine-containing plant extract and/or a plant extract containing polyamines as an active component, there is a method of adding an acid solution to a plant and/or a plant processed product in such a manner as to achieve acidic conditions. The acidic condition means a condition of pH 6 or less. In the extraction, the polyamine composition can be efficiently and stably recovered from plant tissues by adjusting the pH to acidic conditions. This effect can be obtained as long as the pH is 6 or less, preferably 4 or less, and particularly preferably 2 or less. The lower limit is not particularly limited, regardless of the pH of the stock solution of the acid solution used, and is preferably 0 to 2.

By obtaining the plant extract by using acidic conditions (aqueous acid solution), the recovery rate of polyamine amount is higher, and particularly, compound polyamine which is hardly soluble in water can be made soluble in water depending on the acidic conditions, as compared with the plant extract recovered by using an organic solvent such as ethanol or methanol, and the content of free polyamine which is less efficient in extraction by using an organic solvent is also increased. The polyamine exhibits excellent stability under acidic conditions, and the stability of the polyamine and other active components contained in the plant extract is improved. Further, a plant extract is obtained by utilizing acidic conditions (aqueous acid solution), whereby natural active components other than polyamines are simultaneously recovered. Examples of the natural active ingredient include saccharides such as monosaccharides and oligosaccharides, peptides, and proteins. The anti-fouling effect is further enhanced by containing a natural active component other than polyamine and polyamine at the same time. Further, since the recovered plant extract is an aqueous solution, an advantage of higher safety is expected as compared with an organic solvent.

Examples of the acid solution to be added to realize acidic conditions include inorganic acids such as hydrochloric acid, sulfuric acid, and phosphoric acid, organic acids such as acetic acid, propionic acid, butyric acid, oxalic acid, malonic acid, succinic acid, fumaric acid, maleic acid, malic acid, lactic acid, tartaric acid, citric acid, and benzoic acid, and acidic water, inorganic acids such as 0.01 to 6N hydrochloric acid, sulfuric acid, nitric acid, acetic acid, phosphoric acid, trichloroacetic acid, sulfosalicylic acid, formic acid, citric acid, lactic acid, and 0.1 to 10% perchloric acid, and organic acids.

The yield of a polyamine-containing plant extract and/or a plant extract containing polyamines as active ingredients can be increased by adding an acid solution to a plant and/or a processed plant product so as to achieve an acidic condition, or adding a polyphenol adsorbent to the acid solution so as to achieve an acidic condition, followed by crushing, pulverizing, and mixing. In particular, in the case of plant tissues, it is desirable to damage the cell wall because it has a cell wall. In the case of a plant processed product or a plant essence, since it does not contain a cell wall, it is not necessary to specially crush or pulverize the cell wall to damage it. As a method for crushing and pulverizing, for example: blenders, mixers, homogenizers, mortars, ultrasonicators, and the like.

Polyamine contained in a plant and/or a processed plant product is sufficiently extracted in an acid solution (liquid fraction), and then the liquid fraction is separated from a residue or a precipitate by centrifugation or filtration. The liquid fraction thus recovered contains a large amount of polyamine, and "a plant extract containing polyamine and/or a plant extract containing polyamine as an active ingredient" can be obtained.

The polyamine-containing plant extract and/or the plant extract containing polyamine as an active ingredient can be used as an antifouling agent as it is, and is preferably used by compounding with a skin external preparation. The concentration of the polyamine is not particularly limited, and is usually 0.00001 to 100mM, preferably 0.00005 to 75mM, more preferably 0.0001 to 50mM, depending on the degree of absorption, degree of action, product form, frequency of use, and the like.

The antifouling agent and the composition for external application to the skin for preventing the pollution of the present invention contain a polyamine-containing extract derived from a plant as an active ingredient. In the present invention, the anti-contamination means an effect of mitigating damage of the skin caused by air pollutants. More specifically, by preventing or inhibiting, for example: air pollutants adhere to the skin and permeate into the skin to cause inflammation of cells, thereby alleviating skin damage.

The mechanism of action of the anti-contamination effect of the present invention is presumed to be that the plant-derived polyamine-containing extract as the active ingredient of the present invention acts on the inside and outside of epidermal cells of the skin to alleviate the inflammatory action of cells expressed by the adhesion and invasion of air pollutants.

More specifically, it is considered that the anti-contamination effect in the present invention is due to the inhibition of gene expression of IL1 α, IL8, which is an intracellular inflammatory mediator, induced by particulate air pollutants. Further, it is considered that the reason is to suppress the gene expression of the drug metabolizing enzyme CYP1 involved in the induction of expression of these inflammatory mediators.

In the stain-proofing agent and the composition for external application to skin of the present invention, other raw materials may be contained in addition to the plant-derived polyamine-containing extract as an active ingredient within a range that does not impair the effects of the present invention. Examples of such raw materials include water, excipients, antioxidants, preservatives, wetting agents, thickening agents, buffering agents, adsorbents, solvents, emulsifiers, stabilizers, surfactants, lubricants, water-soluble polymers, sweeteners, flavoring agents, souring agents, alcohols and the like. In addition, the stain-proofing agent and the composition for external application to skin of the present invention may contain other active ingredients within a range not impairing the effects of the present invention. As specific examples of the active ingredient, there may be mentioned, for example: antioxidant component, antiaging component, antiinflammatory component, whitening component, cell activating component, vitamins, blood circulation promoting component, moisturizing component, component with effect of preventing and/or repairing DNA damage, anti-glycation component, peptide or its derivative, amino acid or its derivative, hydroquinone glycoside and its ester, etc.

The antifouling agent of the present invention can be used in combination with components and additives for cosmetics, quasi drugs, foods, beverages, medicines and the like, as necessary, in addition to components of a plant-derived polyamine-containing extract, within a range in which the effects of the present invention are not impaired, if necessary.

Examples of the fats and oils include avocado oil, almond oil, anise oil, perilla oil, olive oil, orange oil, new zealand red fish oil (orange roughy oil), sesame oil, cocoa butter, chamomile oil, carrot oil, cucumber oil, beef tallow (beefttail), tallow acid, tung oil, safflower oil, soybean oil, camellia oil, corn oil, rape oil, peach kernel oil, castor oil, cotton seed oil, peanut oil, turtle oil, mink oil, egg yolk oil, cocoa butter, palm oil, palm kernel oil, japan wax, coconut oil, beef tallow, lard, hydrogenated oil, and hydrogenated castor oil.

Examples of waxes include beeswax, carnauba wax, spermaceti wax, lanolin, liquid lanolin, reduced lanolin (reductranolin), hard lanolin, candelilla wax, montan wax, and shellac wax (shellac wax).

Examples of mineral oils include liquid paraffin, vaseline, paraffin, ozokerite (ozokerite), ceresin (ceresin), microcrystalline wax, polyethylene powder, squalene, squalane and pristane (pristine).

Examples of the fatty acid include natural fatty acids such as lauric acid, myristic acid, palmitic acid, stearic acid, behenic acid, oleic acid, 12-hydroxystearic acid, undecylenic acid, tall oil, and wool acid; and synthetic fatty acids such as isononanoic acid, caproic acid, 2-ethylbutyric acid, isovaleric acid, 2-methylvaleric acid, 2-ethylhexanoic acid and isovaleric acid.

Examples of the alcohols include natural alcohols such as ethanol, isopropanol, lauryl alcohol, cetyl alcohol, stearyl alcohol, oleyl alcohol, lanolin alcohol, cholesterol and phytosterol; synthetic alcohols such as 2-hexyldecanol, isostearyl alcohol and 2-octyldodecanol; and polyhydric alcohols such as ethylene oxide, ethylene glycol, diethylene glycol, triethylene glycol, ethylene glycol monoethyl ether, ethylene glycol butyl ether, diethylene glycol monomethyl ether, diethylene glycol monoethyl ether, polyethylene glycol, propylene oxide, propylene glycol, polypropylene glycol, 1, 3-butanediol, glycerol, batyl alcohol, pentaerythritol, sorbitol, mannitol, glucose and sucrose.

Examples of esters include isopropyl myristate, isopropyl palmitate, butyl stearate, hexyl laurate, myristyl myristate, oleyl oleate, decyl oleate, octyldodecyl myristate, hexyldecyl dimethyloctanoate, cetyl lactate, myristyl lactate, diethyl phthalate, dibutyl phthalate, lanolin acetate, ethylene glycol monostearate, propylene glycol monostearate, and propylene glycol dioleate.

Examples of the metal soap include aluminum stearate, magnesium stearate, zinc stearate, calcium stearate, zinc palmitate, magnesium myristate, zinc laurate and zinc undecylenate.

Examples of gum substances (gum mate) and water-soluble polymer compounds include gum arabic, benzoin gum, gum sumac (gum dammar), guaiac, irish moss, karaya gum, tragacanth gum, locust bean gum (gum carob), quince seed (quince seeds), agar, casein, dextran, gelatin, pectin, starch, carrageenan, carboxyalkyl chitin, chitosan, hydroxyalkyl chitin, low molecular weight chitosan, chitosan salts, sulfated chitin, phosphated chitin, alginic acid, alginates, hyaluronic acid, hyaluronate, chondroitin sulfate, heparin, ethylcellulose, methylcellulose, carboxymethyl cellulose, carboxyethyl cellulose, sodium carboxyethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, nitrocellulose, crystalline cellulose, polyvinyl alcohol, polyvinyl methyl ether, polyvinyl pyrrolidone, sodium alginate, gelatin, sodium alginate, polyvinyl methacrylates, polyacrylates, polyalkylene oxides such as polyethylene oxide and polypropylene oxide, crosslinked polymers of polyalkylene oxides, carboxyvinyl polymers, and polyethyleneimines.

Examples of the surfactant include anionic surfactants (carboxylate, sulfonate, sulfate and phosphate), cationic surfactants (amine salt and quaternary ammonium salt), amphoteric surfactants (carboxylic amphoteric surfactant, sulfuric amphoteric surfactant, sulfonic amphoteric surfactant and phosphoric amphoteric surfactant), nonionic surfactants (ether nonionic surfactant, ether-ester nonionic surfactant, block copolymer nonionic surfactant and nitrogen-containing nonionic surfactant), and other surfactants (natural surfactants, derivatives of protein hydrolysates, polymeric surfactants, titanium-silicon-containing surfactants and fluorocarbon surfactants).

Examples of vitamins include: retinol, retinal (vitamin a1), dehydroretinal (vitamin a2), carotene and lycopene (provitamin a) in the vitamin a group; thiamine hydrochloride, thiamine sulfate (vitamin B1), riboflavin (vitamin B2), pyridoxine (vitamin B6), cyanocobalamin (vitamin B12), folic acid, nicotinic acid, pantothenic acid, biotin, choline, and inositol in the vitamin B group; ascorbic acid and ascorbic acid derivatives of the vitamin C group; ergocalciferol (vitamin D2), cholecalciferol (vitamin D3) and tachysterol in the vitamin D group; tocopherols, tocopherol derivatives and ubiquinones from the vitamin E group; and menadione (phytonadione) (vitamin K1), menaquinone (vitamin K2), menadione (vitamin K3) and menadione (vitamin K4) among the vitamin K group.

Examples of amino acids include: valine, leucine, isoleucine, threonine, methionine, phenylalanine, tryptophan, lysine, glycine, alanine, asparagine, glutamine, serine, cysteine, cystine, tyrosine, proline, hydroxyproline, aspartic acid, glutamic acid, hydroxylysine, arginine, ornithine and histidine; and amino acid derivatives such as the aforementioned sulfates, phosphates, nitrates, citrates and pyrrolidone carboxylates.

Examples of the whitening agent include ascorbic acid, ascorbic acid derivatives, sulfur, placenta hydrolysate, ellagic acid derivatives, kojic acid derivatives, glucosamine derivatives, arbutin derivatives, hydroxycinnamic acid derivatives, glutathione, arnica essence, Scutellaria (Scutellaria) root essence, mulberry bark essence, bupleurum (Bupleurum) root essence, Saposhnikovia (Saposhnikovia) root essence, Ganoderma (Ganoderma) mycelium culture, Ganoderma mycelium culture extract, basswood essence, peach leaf essence, rose fruit essence, sophora root essence, Sanguisorba (Sanguisorba officinalis) essence, japanese angelica root essence, coix seed essence, oriental persimmon leaf essence, rhubarb essence, moutan bark essence, hamamelis essence, chestnut tree essence, forsythia suspensa (Hypericum erectum) essence and oil-soluble Glycyrrhiza (Glycyrrhiza) essence.

Examples of the moisturizer include hyaluronic acid, polyglutamic acid, serine, glycine, threonine, alanine, collagen, hydrolyzed collagen, skin-firming glycoprotein (hydrolction), fibronectin, keratin, elastin, royal jelly, heparin chondroitin sulfate, glycerophosphate, glyceroglycolipid, sphingomyelin, glycosphingolipid, linoleic acid, linoleate, eicosapentaenoic acid ester, pectin, Lactobacillus bifidus (Lactobacillus bifidus) fermentation product, lactic acid fermentation product, yeast extract, Ganoderma lucidum (Ganoderma lucidum) mycelium culture, Ganoderma lucidum mycelium culture extract, wheat germ oil, avocado oil, rice germ oil, jojoba oil, soybean phospholipid, gamma-oryzanol, hollyhock essence, coix seed essence, rehmannia root essence, jujube essence, shrimp essence, Aloe arborescens (Aloe arborescens) essence, Aloe vera essence, and Aloe vera essence, Burdock essence, rosemary essence, arnica essence and wheat bran.

Examples of the hair restorer include glyceryl pentadecanoate, Coleus (Coleus) essence, gentian essence, pine cone essence, royal jelly essence, Sasa Veitchii (Sasa veitchii) essence, t-flavanone, 6-benzylaminopurine, Swertia japonica (Swertia japonica) essence, carpronium chloride, minoxidil, finasteride, adenosine, nicotinamide, mulberry root essence, rehmannia root essence, and 5-aminolevulinic acid.

Examples of extracts and essences from animals, plants, and crude drugs include those from: uncaria gambir (Uncaria gambir) (gambir), Angelica keiskei (Angelica keiskei), acerola (acerola), Althea (Althaa), Arnica montana (arnica), avocado, Hydrangea macrophylla (Hydrangea macrophylla Seringevar. thunbergii) (thunbergii Makino) (Hydrangea macrophylla), Aloe (aloe), Aloe vera (aloe vera), Urtica dioica (nettle), Ginkgo biloba (Ginko ba biolo) (Ginkgo biloba and Ginkgo biloba), fennel, Curcuma longa (Asarum sieboldii) (Asarum root of Pinna, Japanese apricot (Japanese apricott), Quercus salicina (Quercus salicina), Berberis uva ursi (Arstaphylos uva-ursi), Scutellaria multiflora (Rosaceae) (bark of Rosaceae), Scutellaria barbata (bark of Rosaceae), Scutellaria (Scutellaria), Scutellaria baicalensis (Scutellaria baicalensis) (Scutellaria), Scutellaria baicalensis (root of Rosaceae) (Scutellaria), Scutellaria baicalensis (root (bark of Rosa), Scutellaria) of Rosaceae (Rosaceae) (Scutellaria), Scutellaria grandiflorum), Scutellaria sinensis (bark of Rosa), Scutellaria (Rosa) (Scutellaria sinensis (Rosa), Scutellaria sinensis (Rosa) of Rosa), Scutellaria (Rosa), Scute, Hypericum erectum (Hypericum erectum), Laminaria sessilifolia (Lamium album), Nasturtium officinale (Nasturtium officinale), Citrus reticulata (orange), Polygala tenuifolia (Polygala tenuifolia) (root of Polygala tenuifolia), Prunella vulgaris (Prunella vulgaris), Polygonum multiflorum (Polygonum multiflorum) (root of Polygonum multiflorum), Sophora japonica (Sophora flower), Artemisia vulgaris (Artemisia Princeps) (folium Artemisiae Argyi), Curcuma zedoaria (Curcumaria zedoaria) (rhizoma Curcumae), Pueraria lobata (Pueraria lobata) (root of Pueraria lobata), Valeriana officinalis (root of Valeriana officinalis), Chrysanthemum morifolium ramosissimum morifolium (Anthemis nobilis), Trichosanthes kirilowii (Trichosanthemum kirilowii) (root of Trichosanthemum japonicum), Artemisia pinnatifida (Rhus chinensis) (flower), Citrus japonica (Rhynchosia japonica) (root of Rosa), Chrysanthemum morinda officinalis (Chrysanthemum morifolium et wilfordii) (root of Chrysanthemum), Chrysanthemum morifolium Rutaceae (Rutaceae) (root of Rosa grandiflorum), Campani) (root of Rutaceae), Campanulaceae (Rutaceae flower of Rosa grandiflorum), Campani (Rutaceae), Campanulaca grandiflorum), Platycodon grandiflorum (root of Campanuml), Campanuml (root, Citrus reticulata (Citrus reticulata) (bark of Citrus reticulata), cucumber, Aralia cordata (Aralia cordiata) and Notopterygium (notopterygium root and Aralia root), almond oil (Prunus armeniaca) (almond), Lycium barbarum (Lycium chinensis) (bark of Lycium barbarum), Lycium barbarum fruit and Lycium barbarum leaf, Sophora flavescens (Sophora flavescens) (root of Picrasma japonica), Cinnamomum camphora (Cinnamomum camphora), Phyllostachys nigra (Sasa veitchii), Citrus grandis (Citrus paradisi) fruit, Cinnamomum zeylanicum (Cinnamomum zeylanicum) (bark of Cinnamomum Cassia), Schizonepeta tenuifolia (Cassia orientalis), Cassia obtusifolia (Cassia obtusifolia) (bark of Cassia japonica), Cassia obtusifolia (Cassia obtusifolia), Phaseolus angustifolia (Trigonolobus grandifolia), and Phaseolus orientalis (Isopteris sativa), Gardenia (Magnolia officinalis) (bark of Myrotheca), Carthamus officinalis (Acipes officinalis), Myrotheca officinalis (Acipes officinalis), Myrotheca, Fructus Schisandrae (Schisandra chinensis) (Schisandra chinensis fruit), rice bran, wheat, Bupleurum scorzonerifolium (Bupleurum chinense), saffron, Saponaria officinalis (Saponaria officinalis), Crataegus cuneata (Crataegus cuneata) (Crataegus hawthorn fruit), Zanthoxylum bungeanum (Zanthoxylum piperitum) (Zanthoxylum bungeanum fruit), Salvia officinalis, Panax notoginseng (Panax notoginseng) (Panax notoginseng root), Lentinus edodes (Lentinula edodes) (Lentinus edodes), Rehmannia glutinosa (Rehmannia glutinosa (Rehmannia root), Quisqualis indica (Quisquassia indica) (Combreviffia seed), Lithospermum erythrorhizomes (Lithospermum erythrorhizon) (Boraginacea root), Perilla frutescens (Perilla occidentalis) (leaf and Perilla frutess orientalis), persimmon (powder), Paeonia lactiflora (Paenia acumia auriculata (Aceris), Plantagia purpura (Aceris purpurea) (Psyllia), Acorus gramineus (Plantago officinalis) (Psyllium officinalis) (Psyllium frugium frutus (Psyllium fru) and leaf (Psyllium frutus) (Acorus calamus (Pilea) and leaf), Acorus calamus (Acorus calamus) and Acorus calamus, Betula pendula (Betula alba), honeysuckle (Lonicera japonica) (honeysuckle and honeysuckle leaves and stems), English catharanthus roseus, yarrow, Sambucus nigra, Ormosia adzuki (red bean), Sambucus nigra (Sambucus sieboldiana)), mallow, Ligusticum wallichii (Ligusticum wallichii), swertia japonica (swertia davidi), Morus alba (Mulberry bark and Mulberry leaf), Zizyphi fructus, Soy bean, Rheum officinale (Aralia elata), Panax japonicus (Panax japonica) (rhizome of Panax japonicus), Anrrhena asphodeloides (Anrrhena asphodeloides) (rhizome of Anrrhena asphodeloides), sanguisorba officinalis (Sanguynia cordifolia), Houttuynia cordata (Houttuynia cordifolia), Cordyceps (Cordycepssinensis) (Cordyceps sinensis (plant)), pepper, Physalis alkekengi) (root of Physalis glauca), Thymus vulgaris, Green tea, black tea, Eugenia caryophylli (Citrus reticulata japonica) (bark, Phyllanthus japonica (Acronychus chinensis), Eugenia japonica (Angelica sinensis (Acipes sinensis (Miq.) DC (Acipes sinensis (Acipes) and/Acipes (Acipes), Calendula (calndiula officinalis), Citrus aurantium (Citrus aurantium), sanguisorba officinalis, corn (corn milk), Eucommia ulmoides (Eucommia ulmoides) (bark and leaf of Eucommia ulmoides), tomato, Nandina domestica (Nandina domestica), garlic, barley (malt), dictamnus (root bark of dictamnus), ophiopogon japonicus (ophiopogonospora), parsley, sweet potato, mint (Mentha arvensis) (mint herb), witch hazel, rose, loquat (eriobotrya japonica) leaf (loguat leaf), poria (porifera cocos) (kernel of poria), grape leaf, muskmelon, linden, peony (moutan bark), lupin, sasanqua, rose hip (yellow plum) (yellow flower), chestnut, maroon (pine needle), peach kernel, honey peach (honey palm), honey palm (honey palm) leaf, honey palm (honey palm) and honey palm (honey palm) leaf), honey palm (honey palm stem, honey palm (honey palm) leaf, honey palm (honey palm) and honey palm stem, honey palm (honey palm) seed), honey palm stem, honey palm (honey palm stem, honey palm, Motherwort (motherwort), cornflower, saxifrage (strawberry geranium), bayberry (myrica), Alnus japonica (Alnus firma), coix (Coixlacryma-jobi var. ma-yuen) (coix seed), Artemisia mongolica (Artemisia mongolia), Artemisia desertorum (Artemisia japonica), lavender, apple fruit, Ganoderma lucidum (ganodera lucidum) (leys. ex. fr.) Karst, lemon fruit, forsythia suspense (forsythia fruit), astragalus mongholicus, geranium herbs, scopoletin (root of scopoletin), cockscomb (comb of cock), bovine/human placenta extract, porcine/bovine stomach/duodenal/intestinal extract/decomposed extract, water-soluble collagen derivatives, hydrolyzed collagen, elastin, hydrolyzed elastin, water-soluble elastin derivatives, silk protein decomposition products, and bovine blood cell protein decomposition products.

Examples of the microorganism culture metabolites include yeast essence, zinc-containing yeast essence, germanium-containing yeast essence, selenium-containing yeast essence, magnesium-containing yeast essence, fermented glutinous rice essence, gymnema extract, and lactic acid fermentation product of non-fat dry milk.

Examples of alpha-hydroxy acids include glycolic acid, citric acid, malic acid, tartaric acid, and lactic acid.

Examples of the inorganic pigments include anhydrous silicic acid, magnesium silicate, talc, kaolin, bentonite, mica, titanium mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, calcium carbonate, magnesium carbonate, yellow iron oxide, red iron oxide, black iron oxide, ultramarine blue, chromium oxide, chromium hydroxide, carbon black, and calamine.

Examples of the ultraviolet absorber include p-aminobenzoic acid derivatives, salicylic acid derivatives, anthranilic acid derivatives, coumarin derivatives, amino acid compounds, benzotriazole derivatives, tetrazole derivatives, imidazoline derivatives, pyrimidine derivatives, dioxane derivatives, camphor derivatives, furan derivatives, pyridone derivatives, nucleic acid derivatives, allantoin derivatives, nicotinic acid derivatives, vitamin B6 derivatives, oxybenzone, benzophenone, guaiazulene, shikonin, baicalin, baicalein, and berberine.

Examples of astringents include lactic acid, tartaric acid, succinic acid, citric acid, allantoin, zinc chloride, zinc sulfate, zinc oxide, calamine, zinc p-phenolsulfonate, potassium aluminum sulfate, resorcinol, ferric chloride, and tannic acid.

Examples of antioxidants include ascorbic acid, ascorbate, stearate, tocopherol ester derivatives, nordihydroguaiaretic acid, Butylated Hydroxytoluene (BHT), Butylated Hydroxyanisole (BHA), p-hydroxyanisole, propyl gallate, sesamol, sesamolin, and gossypol.

Examples of anti-inflammatory agents include ichthyol, indomethacin (indomethacin), kaolin, salicylic acid, sodium salicylate, methyl salicylate, acetylsalicylic acid, diphenhydramine hydrochloride, d/dl camphor, hydrocortisone, guaiazulene, chamazulene, chlorphenamine maleate, glycyrrhizic acid, glycyrrhetate, glycyrrhetinic acid, and glycyrrhetinate.

Examples of germicidal disinfectants include rivanol, sulphur, benzalkonium chloride, benzethonium chloride, methylrosaniline chloride, cresol, calcium gluconate, chlorhexidine gluconate, sulfonamides, mercurochrome, lactoferrin, and hydrolyzed lactoferrin.

Hair conditioners include selenium disulfide, alkylisoquinolinium bromide (alkyl isoquinoline bromide) solution, zinc pyrithione (zinc pyrithione), phencyclamate (biphenamine), dithiane (thiantol), castoreum tincture, tincture of ginger, tincture of capsicum, quinine hydrochloride, strong ammonia, potassium bromate, sodium bromate and thioglycolic acid.

Examples of perfumes include natural animal perfumes such as musk, civet, castoreum and ambergris; plant perfumes such as anise (anisie) essential oil, angelica essential oil, ylang essential oil, orris essential oil, fennel essential oil, citrus essential oil, ylang essential oil, caraway essential oil, cardamom essential oil, guaiazuki essential oil, fennel essential oil, piper spicatum essential oil, cinnamon essential oil, geranium essential oil, copaiba balsam essential oil, coriander essential oil, perilla essential oil, cedar essential oil, citronella essential oil, jasmine essential oil, zingiber essential oil, cedar essential oil, spearmint essential oil, peppermint essential oil, anise essential oil, tuberose essential oil, clove essential oil, orange blossom essential oil, gaultheria essential oil, tuunk essential oil, patchouli essential oil, rose essential oil, cypress essential oil, thujoram essential oil, sandalwood essential oil, bitter orange leaf essential oil, bay essential oil, vetiver essential oil, fingered citron essential oil, pery essential oil, peruvian rosewood essential oil, balsamica essential oil, balsamium essential oil, balsamifera essential oil, balsami, Camphor tree essential oil, citrus essential oil, eucalyptus essential oil, lime essential oil, lavender essential oil, galois essential oil, lemon grass essential oil, lemon essential oil, rosemary essential oil, and japanese mint essential oil; and other synthetic flavors.

Examples of the pigments/colorants include cabbage red pigment, red rice pigment, madder pigment, annatto pigment, sepia pigment, curcumin, sophora flavescens pigment, krill pigment, persimmon pigment, caramel, gold, silver, gardenia pigment, corn pigment, onion pigment, tamarind pigment, spirulina pigment, buckwheat whole plant pigment, cherry pigment, laver pigment, hibiscus pigment, grape juice pigment, calendula pigment, purple sweet potato pigment, taro pigment, shellac pigment and rutin.

Examples of sweeteners include sugar, sweet hydrangea leaf, fructose, arabinose, galactose, xylose, mannose, maltose, honey, glucose, miraculin and monellin (monellin).

Examples of nutritional additives include calcined calcium, cyanocobalamin, yeast, wheat germ, soybean germ, egg yolk powder, hemicellulose and oxymyl red.

In addition, there are hormones, metal-ion blocking agents, pH adjusting agents, chelating agents, antiseptics/antifungals, refrigerants, stabilizers, emulsifiers, animal/plant proteins, decomposed animal/plant proteins, animal/plant polysaccharides, decomposed animal/plant polysaccharides, animal/plant glycoproteins, decomposed animal/plant glycoproteins, blood flow increasing agents, anti-inflammatory agents, anti-allergic agents, cell activators, keratolytic agents, wound healing agents, foaming agents, thickening agents, oral care agents, deodorants, bittering agents, seasonings, enzymes, and the like.

The anti-staining agent of the present invention may be used in any desired dosage form and may be blended to provide quasi-drugs, skin/hair care cosmetics, bath agents, foods, drinks or medicines in the form of ampoules, capsules, powders, granules, pills, tablets, solids, liquids, gels, foams, emulsions, creams, ointments, sheets or mousses, and the like.

Specific examples of cosmetic and quasi-drug products include internal/external pharmaceutical preparations; basic cosmetics, face cleansers and skin cleansers such as lotions, creams, ointments, lotions, oils and masks; hair cosmetics such as shampoo, rinse (rinse), hair care, hair cream, hair wax, hair gel, hair styling agent (hair dressing), hair setting solution (permanent solution), hair tonic, hair dye, and hair restorer; makeup cosmetics (make-up cosmetics) such as foundation cream, white powder (white powder), face powder (face powder), lipstick, blush, eye shadow, eyeliner (liquid), mascara, eyebrow pencil, and false eyelashes; cosmetic preparations such as manicure; a perfume; bath agents, dentifrices; breath fresheners/mouthwashes; liquid odor inhibitors/deodorants; a sanitary article; sanitary napkins and wet wipes.

Examples of the food and drink products include drink products such as refreshing beverages, carbonated beverages, nutritional supplement beverages, fruit juice beverages, and lactic acid beverages; freezing points such as ice cream, sherbet and water ice; noodles such as buckwheat noodles, wheat noodles, vermicelli, dumpling (dumpling and steamed dumpling) skin, Chinese noodles and instant noodles; confectionery (confectioneries) such as fruit hard candies (drop), candies (confections), chewing gums, chocolates, tableted candies, snacks, biscuits, jellies, jams, creams, baked goods and breads; aquatic products such as crab, salmon, mackerel, tuna, sardine, shrimp, bonito, mackerel, whale, oyster, saury, squid, red shellfish, scallop, abalone, sea urchin, salmon caviar, and abalone shell; fishery/livestock processed foods such as fish sausage, ham and sausage; dairy products such as milk powder, processed milk and fermented milk; oils/fats and processed oils/fats foods such as salad oil, frying oil, margarine, mayonnaise, shortening, whipped cream and sauces; seasonings such as soy sauce and sauce (dip); steaming and boiling bag food such as curry, stew, grilled chicken and egg rice, porridge containing vegetables or fish, fried mixed rice, fried pork chop rice, fried fish or vegetable rice, grilled eel rice, meat sauce rice, Japanese mixed rice (Kanto cooking), spicy bean curd, seasoned beef rice, meat soup, egg soup, omelet seasoned rice, dumpling (dumpling and shaomai), hamburger and meat ball; various forms of health/nutritional supplement foods; promoting health food; a tablet; capsules; nutritional supplement drinks and lozenges.

The antifouling agent of the present invention and the composition for external application to skin containing the same can be suitably used for humans, and can also be used for animals other than humans, and their respective effects can be expected as much as possible.

The embodiment of the composition for external application to the skin of the present invention is not particularly limited, and examples thereof include: face washes, cleansers, lotions (e.g., whitening lotions), creams (e.g., vanishing creams, cold creams), lotions, gels, beauty lotions, face masks (e.g., jelly-like frostings, pasty wiping-off, powdered rinse-off), sheet masks, makeup removers, foundations, lipsticks, lip gloss, lip pencils, blush, pre-makeup creams, shaving creams, sun creams, after-sun lotions, deodorizing lotions, body lotions (including hand creams, foot creams), body oils, and the like. From the viewpoint of anti-staining, particularly preferred is a skin external composition to be used in a leave-on-day type, and examples thereof include lotions, creams, lotions, gels, beauty lotions, foundations, pre-creams, sun screens, body lotions, body oils, and the like.

Hereinafter, the present invention will be described in more detail based on examples. The present invention is not particularly limited to the examples.

Examples

Example 1 preparation of polyamine-containing plant extract and/or plant extract containing polyamine as active ingredient from soybean germ

To 1kg of soybean germ (For you, inc.) was added diluted ginseng solution (1, 7-diaminoheptane, internal parameter 10 μmol), 5L of 1N hydrochloric acid solution was added, and the mixture was left at room temperature For 1 hour. Then, 80g of PolyclarVT (ISP) as a polyphenol adsorbent was added thereto, and the soybean germs were sufficiently crushed by a homogenizer and then left at room temperature for 1 hour to be extracted under acidic conditions. The crushed material was centrifuged at 22,000 Xg for 30 minutes at 2 ℃ and a liquid fraction was extracted, neutralized with 30% sodium hydroxide solution and the liquid was used as a plant extract (soybean germ extract). The plant extract (soybean germ extract) was desalted using an electrodialysis device (acilzer, manufactured by Astom) and concentrated by freeze-drying. This powder was dissolved in a 30% butanediol solution to 5.0%, and used as a soybean extract for various evaluations.

(example 2) evaluation of expression suppressing action on target Gene

The above-mentioned soybean extract was used as a test sample, and the expression inhibitory effect of the target gene was evaluated by the following test method. The target genes in this example refer to IL1 α gene, IL8 gene and CYP1 gene.

In-use glueA Collagen-coated T75 flask was inoculated with human normal neonatal epidermal keratinocytes (NHEK) in a Collagen-coated original protein Coating Solution (manufactured by Toyo Kabushiki Kaisha), and a medium (Humedia-KG2) for proliferating normal human epidermal keratinocytes was added thereto at 37 ℃ in the presence of 10% CO₂Culturing was carried out by appropriately replacing the medium, culturing was continued for 8 days until confluence of 80% or more was achieved, removing the medium and rinsing with PBS, trypsinizing and recovering the cells, inoculating the cells in a 96-well plate to 1 × 10⁴Cells/well, 10% CO at 37 ℃₂The culture was carried out for 1 day.

The medium was removed and replaced with medium containing the sample to be tested at a given concentration, 10% CO at 37 deg.C₂The culture was conditioned for 5 hours. In addition to the samples tested, dexamethasone, known as an anti-inflammatory agent, was dissolved in DMSO and used as a positive control (final concentration 1 μ M). PM2.5 extract (200mg/ml) was added to a final concentration of 0.2mg/ml, and the mixture was further cultured for 2 hours. PM2.5 extract, by adding 200mg of city atmospheric dust (NIES CRM No.28) to DMSO1mL, stirring, centrifugation, and collection of supernatant. After the culture, the cells were collected and Cell Lysis was performed using SuperPrep (registered trademark)&An RTqPCR kit (manufactured by toyobo co., ltd.) was prepared from cells.

Based on the obtained cDNA, mRNA gene was amplified by qRT-PCR using THUNDERBIRD SYBR (registered trademark) qPCR Mix (manufactured by Toyobo Co., Ltd.). The composition of the reaction solution was in accordance with the attached document of the kit. The reaction solution volume was set at 20. mu.l/well and the cDNA volume was set at 3. mu.l. The reaction cycle conditions were carried out at 95 ℃ for 1 minute → (95 ℃, 15 seconds → 60 ℃, 45 seconds) × 40 → 95 ℃, 15 seconds → 60 ℃, 1 minute → 95 ℃, 15 seconds. The primer used contains an oligonucleotide having a base sequence represented by SEQ ID Nos. 1 to 8. Gene amplification used a 7500 Rapid real-time PCR System (Applied Biosystems). The glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene was used as an internal control, and the expression level of the target gene was normalized. Relative values were calculated by dividing the normalized expression level of each target gene by the expression level of the gene (untreated control) confirmed in the same manner as described above except that no PM2.5 was added.

The results obtained are shown in FIGS. 1 to 3. As can be seen from fig. 1 to 3, in NHEK, in the negative control to which no soybean extract was added, the particulate air pollutants excited the expression levels of IL1 α gene, IL8 gene and CYP1 gene more than in the untreated control, and it was confirmed that the expression levels of IL1 α gene, IL8 gene and CYP1 gene were reduced by the addition of the plant-derived polyamine-containing extract. From the results, it was found that the plant-derived polyamine-containing extract of the present invention suppressed the hyperactivity of IL1 α gene, IL8 gene and CYP1 gene induced by PM2.5 in epidermal cells, and suppressed inflammatory response.

Example 3 preparation of composition for external application to skin

Hereinafter, examples of the prescription of the present invention as an external preparation for skin are shown.

(1) Toning lotion

A toner was produced according to a conventional method with the following composition.

89.80g of purified water

3.00g of glycerin

0.20g of phenoxyethanol

5.00g of butanediol

1.00g of pentanediol

Soybean extract 1.00g

(2) Gel

Gels were made according to conventional methods with the following composition.

88.50g of purified water

Carbomer (carbomer) 0.30g

Xanthan gum 0.10g

Arginine 0.40g

5.00g of glycerin

0.20g of phenoxyethanol

5.00g of butanediol

0.50g of soybean extract

(3) Cream

The cream was produced according to a conventional method according to the following composition.

58.50g of purified water

10.00g of butanediol

5.00g of glycerin

0.20g of phenoxyethanol

0.20g of ethylhexylglycerin

Squalane 10.00g

10.00g of olive oil

Docosanol 2.50g

Polyglyceryl-10 pentastearate 1.90g

Sodium stearyl lactate 0.60g

Palmitic acid cetyl ester 1.00g

0.10g of Soybean extract

Industrial applicability

Any of these agents is expected to have an effect of inhibiting the expression of IL1 α gene, IL8 gene and CYP1 gene whose expression is stimulated by PM2.5 in epidermal cells, and is effective as an antifouling agent. The antifouling agent is particularly effective as a component of an external preparation for skin.

Claims (6)

1. An antifouling agent comprising a polyamine-containing extract of plant origin as an active ingredient.

2. The stain resistant agent according to claim 1, wherein the polyamine-containing extract of plant origin is an extract of at least one or more material sources selected from the group consisting of soybean seeds, soybean germs, soybean sprouts, wheat seeds, wheat germs, wheat malt, soybean milk, and soybean dregs.

3. Stain repellent according to claim 1 or 2, wherein the polyamine-containing extract of vegetable origin is an extract of soy germ origin.

4. The antifouling agent according to any one of claims 1 to 3, which contains at least one or more compounds selected from the group consisting of putrescine, spermidine and spermine as the polyamine.

5. The anti-staining agent according to any one of claims 1-4, which has the effect of inhibiting the expression of at least 1 gene selected from the group consisting of interleukin-1 a (IL1 a), interleukin-8 (IL8) and cytochrome P450(CYP 1).

6. An external composition for skin, which contains the antifouling agent as claimed in any one of claims 1 to 5.

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