CN111675923A - Method for extracting gardenia pigment through steam explosion and application method of extracted pigment - Google Patents
Method for extracting gardenia pigment through steam explosion and application method of extracted pigment Download PDFInfo
- Publication number
- CN111675923A CN111675923A CN202010587325.XA CN202010587325A CN111675923A CN 111675923 A CN111675923 A CN 111675923A CN 202010587325 A CN202010587325 A CN 202010587325A CN 111675923 A CN111675923 A CN 111675923A
- Authority
- CN
- China
- Prior art keywords
- gardenia
- pigment
- steam explosion
- steam
- extracting
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B61/00—Dyes of natural origin prepared from natural sources, e.g. vegetable sources
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/111—Aromatic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/179—Colouring agents, e.g. pigmenting or dyeing agents
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Birds (AREA)
- Organic Chemistry (AREA)
- Fodder In General (AREA)
Abstract
The invention provides a method for extracting gardenia pigment by steam explosion and application of the extracted pigment, the extraction method is simpler, and the poultry use is not influenced. A method for extracting gardenin by steam explosion comprises (1) performing steam explosion treatment on Gardenia; (2) extracting the steam exploded fructus Gardeniae with ethanol under reflux, filtering, recovering ethanol from the filtrate, concentrating the recovered residual solution, and spray drying to obtain fructus Gardeniae pigment dry powder. The gardenia pigment prepared based on the steam explosion technology mainly plays a role in coloring as a feed additive for laying fowls, deepens the color of egg yolks of eggs, and greatly meets the requirements of consumers on the quality of the eggs. The gardenia pigment is used as a feed additive to feed laying poultry directly, so that the subsequent complicated refining step can be omitted, and the application field of the gardenia pigment is expanded.
Description
Technical Field
The invention belongs to the field of natural pigment preparation methods and application, and particularly relates to a method for extracting gardenia pigment through steam explosion and an application method of the extracted pigment.
Background
The natural pigment is a colorant in food additives, and most of the natural pigments have biological activity and nutrition strengthening effect. If carotene is listed as a nutritional additive abroad, the U.S. has applied carotene to infant food; some have pharmacological action and health-care function of preventing and curing some diseases of human body, for example, anthocyanin has the functions of diminishing inflammation, resisting tumor, removing oxygen free radical, inhibiting lipoprotein oxidation and platelet aggregation. The development of research, development and application of natural edible pigment is rapid, and the natural edible pigment is a new trend of food additive development.
Yolk coloration is an important sensory index for measuring the quality of eggs, and is an index for consumers to subjectively evaluate the quality of eggs. From the economic perspective of poultry industry, the brightness of egg yolk color directly affects the commercial value of poultry eggs.
The gardenia is a traditional Chinese medicine, belongs to the 1 st batch of medicine and food dual-purpose resources issued by the Ministry of health, is cold in nature and bitter in taste, enters heart, lung, stomach and triple energizer channels, and has the effects of clearing heat and cooling blood, detoxifying and promoting diuresis, purging pathogenic fire and relieving restlessness, promoting urination and the like. The gardenia contains components such as polysaccharide, volatile oil, geniposide and the like, and pigment components of the gardenia mainly comprise terpenoid crocin and flavonoid gardenia yellow pigment which can be used as dyes, belong to natural pigments, and have high safety, good stability and no toxic or side effect. Therefore, the demand of gardenia yellow pigment is gradually increased at home and abroad.
Gardenin has been conventionally used for coloring of colored flour products in food processing, and because gardenin contained therein tends to make the colored flour products turn green, it is necessary to refine the colored flour products during extraction. In the prior art, a plurality of methods for extracting gardenia pigment exist, and Chinese patent application No. CN201210184325.0 discloses a method for preparing gardenia pigment from gardenia, the method adopts the processes of peeling, extraction, concentration, filtration, macroporous resin purification, elution, concentration, drying and the like to prepare gardenia yellow pigment and gardenoside, and the gardenoside is prepared into gardenia blue or gardenia red pigment through the biological engineering technology, the membrane filtration and the acid precipitation method, so that the production cost of the gardenia yellow pigment is reduced, the comprehensive utilization level of the raw materials is improved, and the prepared series of pigments can be applied to the fields of food, health care products, cosmetics, medicines and the like. The method mainly adopts conventional extraction method with addition of macroporous resin, membrane filtration and acid precipitation to obtain gardenin, and has the advantages of complicated process and low yield.
Chinese patent application No. CN201210029611.X discloses a process for extracting gardenia yellow pigment, which comprises the steps of adding petroleum ether for ultrasonic extraction, then carrying out ultrasonic extraction by using ethanol, then loading the obtained product into a macroporous adsorption resin column, washing the column by using deionized water, eluting the pigment by using ethanol to obtain crude gardenia yellow pigment, loading the crude gardenia yellow pigment into a polyamide column, eluting the crude gardenia yellow pigment by using ethanol, and concentrating the ethanol to obtain the gardenia yellow pigment. The method has a complex process, and needs to use more organic reagents for washing in the process of applying the macroporous adsorption resin except that petroleum ether is used for ultrasonic treatment, and reagent residues are easy to exist if the removal is not thorough.
Chinese patent application No. CN201410012353.3 discloses a preparation method of gardenia yellow pigment, which adopts compound enzymolysis, macroporous adsorption resin refining, concentration and crystallization to obtain the gardenia yellow pigment. The method reduces pigment loss caused by processing procedures and concentration times, and the obtained product has good quality and low energy consumption, and is convenient for industrial production. However, the macroporous adsorption resin needs more solvent for elution, the solvent recovery cost is increased, the equipment cost and the energy consumption are increased, a large amount of adsorption resin is needed during the process production, and the cost is relatively increased.
Disclosure of Invention
In order to solve the problems, the invention provides a method for extracting gardenia pigment by steam explosion and application of the extracted pigment, the extraction method is simpler, and the poultry use is not influenced.
The object of the invention is achieved in the following way: a method for extracting gardenin by steam explosion comprises (1) performing steam explosion treatment on Gardenia; (2) extracting the steam exploded fructus Gardeniae with ethanol under reflux, filtering, recovering ethanol from the filtrate, concentrating the recovered residual solution, and spray drying to obtain fructus Gardeniae pigment dry powder.
The step (1) is that firstly, the gardenia is put into a steam explosion device, saturated steam is introduced inwards, the steam pressure is maintained at 3-10 Mpa, and the steam explosion is carried out for 2-6 min, so as to obtain the steam explosion gardenia.
In the step (2), the extraction conditions of the gardenia subjected to steam explosion are that the concentration of ethanol for reflux is 70-80%, the ethanol is refluxed for 2 times, 20-30min is carried out each time, and filtered filtrates are combined and recovered until no alcohol smell exists.
A method for applying gardenia pigment extracted by steam explosion in poultry breeding comprises adding the gardenia pigment serving as a feed additive into daily ration of laying poultry to feed the laying poultry.
The laying fowl can be chicken, duck or goose.
The gardenia pigment is used as a feed additive and is added into daily ration according to the weight ratio of 0.05-0.1% in the early egg laying period and the egg laying period of laying fowls.
Compared with the prior art, the gardenia pigment prepared based on the steam explosion technology mainly plays a role in coloring as the laying bird feed additive, deepens the color of egg yolks of eggs, and greatly meets the requirements of consumers on the quality of egg products. The gardenia pigment is used as a feed additive to feed laying poultry directly, so that the subsequent complicated refining step can be omitted, and the application field of the gardenia pigment is expanded.
The invention has the beneficial effects that:
1. compared with the existing method for extracting the gardenia pigment, the method obtains the gardenia pigment dry powder by steam explosion, alcohol extraction and spray drying, the steam explosion breaks the glycosidic bond in the gardenia fiber from the molecular level, so that the effective ingredients wrapped by the cellulose are fully released, the yield is high, the process is simple, the reagent only uses water and ethanol, the ethanol can be recycled, and the extraction period is short.
2. The invention takes pigment dry powder prepared by reflux extraction, concentration and spray drying of gardenia after wall breaking as a feed additive to be added into daily ration of laying poultry, and the color of egg yolk obviously turns yellow when the poultry is observed after a period of feeding. The color and the egg weight of the eggshell can be improved, the requirements of consumers on the quality of eggs are greatly met, the nutritive value of the eggs is improved, the cost is reduced, the use of synthetic pigment is avoided, and the food safety of the eggs is enhanced.
3. Because the gardenia has the effects of clearing heat and cooling blood, detoxifying and promoting diuresis, purging fire and relieving restlessness, promoting urination and the like, the gardenia dregs after the pigment is extracted still have the use value, and can be used for daily health care of livestock and poultry by adding the gardenia dregs into the daily ration of the livestock and poultry, so that the gardenia resource is utilized to the maximum extent, the medicinal value is exerted, and the method is simple and feasible.
Detailed Description
A method for extracting gardenin by steam explosion comprises (1) performing steam explosion treatment on Gardenia; (2) extracting the steam exploded fructus Gardeniae with ethanol under reflux, filtering, recovering ethanol from the filtrate, concentrating the recovered residual solution, and spray drying to obtain fructus Gardeniae pigment dry powder.
The step (1) is that firstly, gardenia is put into a steam explosion device, saturated steam is introduced inwards, the steam pressure is maintained at 3-10 Mpa for 2-6 min, and an exhaust valve is opened to release the pressure in the device instantly to obtain the steam explosion gardenia.
In the step (2), the extraction conditions of the gardenia subjected to steam explosion are that the concentration of ethanol for reflux is 70-80%, the ethanol is refluxed for 2 times, 20-30min is carried out each time, filtered filtrates are combined and recovered until no alcohol smell exists, and the residual liquid is reserved.
A method for applying gardenia pigment extracted by steam explosion in poultry breeding comprises adding the gardenia pigment serving as a feed additive into daily ration of laying poultry to feed the laying poultry.
The laying fowl can be chicken, duck or goose.
The gardenia pigment is used as a feed additive and is added into daily ration according to the amount of 0.05-0.1% in the early egg laying period and the egg laying period of laying poultry.
The specific producer and model of the steam explosion device of this application: gecko biological energy Co., Ltd QB 1500.
The gardenia yellow pigment is traditionally used for coloring the coloring flour products in food processing, and the gardenia yellow pigment is easy to make the flour products dyed with the gardenia yellow pigment green due to the gardenoside contained in the flour products and needs to be refined during extraction. The gardenia yellow pigment is used as a feed additive to feed laying poultry directly, so that the subsequent complicated refining step can be omitted, and the application field of the gardenia yellow pigment is expanded.
The present invention is described in detail below with reference to specific embodiments, it should be noted that the embodiments are only used for further illustration of the present invention, and should not be construed as limiting the scope of the present invention, and those skilled in the art can make modifications and adaptations of the present invention based on the above-mentioned disclosure.
Example 1
A method for extracting gardenin by steam explosion comprises the following steps,
(1) firstly, filling the gardenia into a steam explosion device, introducing saturated steam inwards, keeping the steam pressure at 3MPa, and opening an exhaust valve after 2min to instantly release the pressure in the device to obtain the steam explosion gardenia.
(2) Extracting fructus Gardeniae with 70% ethanol under reflux for 20 min, filtering, extracting again, recovering ethanol from the combined filtrates, concentrating the residual solution, and spray drying to obtain dry powder.
The application method of gardenia pigment extracted by steam explosion in poultry breeding comprises the step of adding gardenia pigment dry powder serving as a feed additive into daily ration of laying poultry according to the weight ratio of 0.05-0.1%.
Example 2
A method for extracting gardenin by steam explosion comprises the following steps,
(1) firstly, filling the gardenia into a steam explosion device, introducing saturated steam into the steam explosion device, maintaining the steam pressure at 7MPa for 4min, and opening an exhaust valve to release the pressure in the device instantly to obtain the steam explosion gardenia.
(2) Extracting fructus Gardeniae with 75% ethanol under reflux for 20 min, filtering, extracting again, mixing filtrates, recovering ethanol, concentrating the residual solution, and spray drying to obtain dry powder.
The application method of gardenia pigment extracted by steam explosion in poultry breeding comprises the step of adding gardenia pigment dry powder serving as a feed additive into daily ration of laying poultry according to the weight ratio of 0.05-0.1%.
Example 3
A method for extracting gardenin by steam explosion comprises the following steps,
(1) firstly, filling the gardenia into a steam explosion device, introducing saturated steam inwards, keeping the steam pressure at 10MPa, and opening an exhaust valve after 6min to instantly release the pressure in the device to obtain the steam explosion gardenia.
(2) Extracting fructus Gardeniae with 80% ethanol under reflux for 30min, filtering, extracting again, recovering ethanol from the combined filtrates, concentrating the residual solution, and spray drying to obtain dry powder of gardenin.
The application method of gardenia pigment extracted by steam explosion in poultry breeding comprises the step of adding gardenia pigment dry powder serving as a feed additive into daily ration of laying poultry according to the weight ratio of 0.05-0.1%.
Test verification
To further demonstrate the effect of the present invention, the following comparative tests were performed: selecting 560 test chickens, dividing the test chickens into 7 groups including a control group and 6 experimental groups, wherein each group comprises 80 chickens, freely eating drinking water under the same feeding condition, feeding conventional chicken feed (corn-soybean meal type feed) to the control group, and feeding conventional chicken feed (corn-soybean meal type feed) respectively added with the gardenia pigment dry powder of the examples 1-3 to the experimental groups 1, 2 and 3; experiment 4 groups of conventional chicken feeds (corn-soybean meal type feeds) were fed with a feed additive lemon yellow produced by Shandong Guo Shuo Biotech Co., Ltd; experiment 5 groups of conventional chicken feeds (corn-soybean meal type feeds) were supplemented with directly crushed gardenia powder; experiment 6 groups of conventional chicken feeds (corn-soybean meal type feeds) were fed with refined gardenia yellow pigment for food coloring produced by yunnan mairei biotechnology limited; the color of the egg yolk is measured by Roche colorimetric fan (RCF value) for 1 month, and the nutrition status, egg shell strength and laying rate of the chicken are observed. The control group and the 6 experimental groups were fed with the same conventional chicken feed (corn-soybean meal type feed), and the number of times of feeding per day and the amount of feeding were the same.
The results of the experiment are as follows:
remarking: the Roche color comparison fan method is a current universal method for measuring the color of the yolk, is expressed by RCF value, is simple, practical and quick, is recognized as the international yolk quality standard, and is divided into 15 grades, the higher the grade number is, the darker the color is.
Experiments show that the laying hens in the control group, the experimental group 4 and the experimental group 6 have the conditions of diarrhea, inappetence and the like in a certain proportion, the laying rate is reduced, and the eggshells have the phenomena of cracks, soft shells and thin shells; the feed added by feeding the chicken feed can obviously improve the color and the quality of egg yolk, enhance the resistance of chicken bodies, reduce the morbidity, promote the growth of the chicken and improve the laying rate. The experimental group chickens added with the crushed gardenia powder have no conditions of inappetence, diarrhea and the like, but have poor coloring effect on egg yolk, and the effect is consistent with that of the experimental group chickens added with the crushed gardenia powder, the experimental group 6 is added with refined gardenia yellow pigment, the color of the egg yolk is obviously higher than that of a control group, but 5% of the chickens have the conditions of diarrhea, inappetence and the like, and the laying rate and the eggshell strength are basically consistent with those of the experimental group 4. The analysis reason may be that the commercially available refined gardenia yellow pigment is mainly used for coloring the colored flour products in food processing, the flour products dyed with the gardenia yellow pigment are easy to turn green due to the fact that the flour products contain more gardenoside, the gardenoside needs to be refined to remove during extraction, the technologies such as macroporous adsorption resin, extraction and the like are mostly adopted, meanwhile, more organic reagents are used during washing and elution, although gardenoside is removed and the pigment is purified, but the gardenia is used as a common traditional Chinese medicine, and the active ingredients in the gardenia yellow pigment are greatly lost. If the method is applied to the field of poultry breeding, compared with the method, the method for refining the gardenia yellow pigment firstly adds a refining step, has more complex process and higher cost, and secondly, although the color of the yolk is improved, the quality of the eggshell and the laying rate are reduced by a certain proportion, so that the breeding cost is higher for most farmers.
In conclusion, the extraction method of the gardenia yellow pigment for the egg-laying poultry breeding field is simple and feasible, the effective components of the traditional Chinese medicine gardenia can be released to the maximum extent through the steam explosion technology, the crude gardenia pigment obtained by spray drying the extracting solution can be applied to the egg-laying poultry breeding field, the extraction steps of the pigment are simplified, meanwhile, the gardenia serving as other effective components of the traditional Chinese medicine is reserved, the value of the gardenia is fully utilized, and the breeding cost is reduced.
Embodiments of the present application may also be the following embodiments.
Example 4
A method for extracting fructus Gardeniae pigment by steam explosion comprises (1) loading fructus Gardeniae into steam explosion device, introducing saturated steam, maintaining steam pressure at 3Mpa for 6min, and opening exhaust valve to release pressure in the device instantly to obtain steam-exploded fructus Gardeniae; (2) extracting fructus Gardeniae with 70% ethanol under reflux for 20 min for 2 times, filtering, recovering ethanol from filtrate, concentrating the residual solution, and spray drying to obtain dry powder of fructus Gardeniae pigment.
The application method of gardenia pigment extracted by steam explosion in poultry breeding comprises the step of adding gardenia pigment dry powder serving as a feed additive into daily ration of laying poultry according to the weight ratio of 0.05-0.1%.
Example 5
A method for extracting fructus Gardeniae pigment by steam explosion comprises (1) loading fructus Gardeniae into steam explosion device, introducing saturated steam, maintaining steam pressure at 4Mpa for 3min, and opening exhaust valve to release pressure in the device instantly to obtain steam-exploded fructus Gardeniae; (2) extracting fructus Gardeniae with 80% ethanol under reflux for 20 min for 2 times, filtering, recovering ethanol from filtrate, concentrating the residual solution, and spray drying to obtain dry powder of fructus Gardeniae pigment.
The application method of gardenia pigment extracted by steam explosion in poultry breeding comprises the step of adding gardenia pigment dry powder serving as a feed additive into daily ration of laying poultry according to the weight ratio of 0.05-0.1%.
Example 6
A method for extracting fructus Gardeniae pigment by steam explosion comprises (1) loading fructus Gardeniae into steam explosion device, introducing saturated steam, maintaining steam pressure at 6Mpa for 2min, and opening exhaust valve to release pressure in the device instantly to obtain steam-exploded fructus Gardeniae; (2) extracting fructus Gardeniae with 70% ethanol under reflux for 30min for 2 times, filtering, recovering ethanol from filtrate, concentrating the residual solution, and spray drying to obtain dry powder of fructus Gardeniae pigment.
The application method of gardenia pigment extracted by steam explosion in poultry breeding comprises the step of adding gardenia pigment dry powder serving as a feed additive into daily ration of laying poultry according to the weight ratio of 0.05-0.1%.
Example 7
A method for extracting fructus Gardeniae pigment by steam explosion comprises (1) loading fructus Gardeniae into steam explosion device, introducing saturated steam, maintaining steam pressure at 8Mpa for 4min, and opening exhaust valve to release pressure in the device instantly to obtain steam exploded fructus Gardeniae; (2) extracting fructus Gardeniae with 80% ethanol under reflux for 30min for 2 times, filtering, recovering ethanol from filtrate, concentrating the residual solution, and spray drying to obtain dry powder of fructus Gardeniae pigment.
The application method of gardenia pigment extracted by steam explosion in poultry breeding comprises the step of adding gardenia pigment dry powder serving as a feed additive into daily ration of laying poultry according to the weight ratio of 0.05-0.1%.
Example 8
A method for extracting fructus Gardeniae pigment by steam explosion comprises (1) loading fructus Gardeniae into steam explosion device, introducing saturated steam, maintaining steam pressure at 9Mpa for 3min, and opening exhaust valve to release pressure in the device instantly to obtain steam-exploded fructus Gardeniae; (2) extracting fructus Gardeniae with 75% ethanol under reflux for 30min for 2 times, filtering, recovering ethanol from filtrate, concentrating the residual solution, and spray drying to obtain dry powder of fructus Gardeniae pigment.
The application method of gardenia pigment extracted by steam explosion in poultry breeding comprises the step of adding gardenia pigment dry powder serving as a feed additive into daily ration of laying poultry according to the weight ratio of 0.05-0.1%.
Example 9
A method for extracting fructus Gardeniae pigment by steam explosion comprises (1) loading fructus Gardeniae into steam explosion device, introducing saturated steam, maintaining steam pressure at 5Mpa for 6min, and opening exhaust valve to release pressure in the device instantly to obtain steam-exploded fructus Gardeniae; (2) extracting fructus Gardeniae with 80% ethanol under reflux for 20 min for 2 times, filtering, recovering ethanol from filtrate, concentrating the residual solution, and spray drying to obtain dry powder of fructus Gardeniae pigment.
The application method of gardenia pigment extracted by steam explosion in poultry breeding comprises the step of adding gardenia pigment dry powder serving as a feed additive into daily ration of laying poultry according to the weight ratio of 0.05-0.1%.
Example 10
A method for extracting fructus Gardeniae pigment by steam explosion comprises (1) loading fructus Gardeniae into steam explosion device, introducing saturated steam, maintaining steam pressure at 5Mpa for 5min, and opening exhaust valve to release pressure in the device instantly to obtain steam exploded fructus Gardeniae; (2) extracting fructus Gardeniae with 75% ethanol under reflux for 20 min for 2 times, filtering, recovering ethanol from filtrate, concentrating the residual solution, and spray drying to obtain dry powder of fructus Gardeniae pigment.
The application method of gardenia pigment extracted by steam explosion in poultry breeding comprises the step of adding gardenia pigment dry powder serving as a feed additive into daily ration of laying poultry according to the weight ratio of 0.05-0.1%.
Example 11
A method for extracting fructus Gardeniae pigment by steam explosion comprises (1) loading fructus Gardeniae into steam explosion device, introducing saturated steam, maintaining steam pressure at 7Mpa for 4.5min, and opening exhaust valve to release pressure in the device instantly to obtain steam exploded fructus Gardeniae; (2) extracting fructus Gardeniae with 70% ethanol under reflux for 20 min for 2 times, filtering, recovering ethanol from filtrate, concentrating the residual solution, and spray drying to obtain dry powder of fructus Gardeniae pigment.
The application method of gardenia pigment extracted by steam explosion in poultry breeding comprises the step of adding gardenia pigment dry powder serving as a feed additive into daily ration of laying poultry according to the weight ratio of 0.05-0.1%.
Example 12
A method for extracting fructus Gardeniae pigment by steam explosion comprises (1) loading fructus Gardeniae into steam explosion device, introducing saturated steam, maintaining steam pressure at 3.5Mpa for 4.5min, and opening exhaust valve to release pressure in the device instantly to obtain steam exploded fructus Gardeniae; (2) extracting fructus Gardeniae with 76% ethanol under reflux for 30min for 2 times, filtering, recovering ethanol from filtrate, concentrating the residual solution, and spray drying to obtain dry powder of fructus Gardeniae pigment.
The application method of gardenia pigment extracted by steam explosion in poultry breeding comprises the step of adding gardenia pigment dry powder serving as a feed additive into daily ration of laying poultry according to the weight ratio of 0.05-0.1%.
Example 13
A method for extracting fructus Gardeniae pigment by steam explosion comprises (1) loading fructus Gardeniae into steam explosion device, introducing saturated steam, maintaining steam pressure at 5.5Mpa for 3.5min, and opening exhaust valve to release pressure in the device instantly to obtain steam exploded fructus Gardeniae; (2) extracting fructus Gardeniae with 74% ethanol under reflux for 30min for 2 times, filtering, recovering ethanol from filtrate, concentrating the residual solution, and spray drying to obtain dry powder of fructus Gardeniae pigment.
The application method of gardenia pigment extracted by steam explosion in poultry breeding comprises the step of adding gardenia pigment dry powder serving as a feed additive into daily ration of laying poultry according to the weight ratio of 0.05-0.1%.
Example 14
A method for extracting fructus Gardeniae pigment by steam explosion comprises (1) loading fructus Gardeniae into steam explosion device, introducing saturated steam, maintaining steam pressure at 6.8Mpa for 5.5min, and opening exhaust valve to release pressure in the device instantly to obtain steam exploded fructus Gardeniae; (2) extracting fructus Gardeniae with 78% ethanol under reflux for 30min for 2 times, filtering, recovering ethanol from filtrate, concentrating the residual solution, and spray drying to obtain dry powder of fructus Gardeniae pigment.
The application method of gardenia pigment extracted by steam explosion in poultry breeding comprises the step of adding gardenia pigment dry powder serving as a feed additive into daily ration of laying poultry according to the weight ratio of 0.05-0.1%.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various changes and modifications can be made without departing from the overall concept of the present invention, and these should also be considered as the protection scope of the present invention.
Claims (6)
1. A method for extracting gardenia pigment by steam explosion is characterized by comprising the following steps: comprises the following steps of (1) carrying out steam explosion treatment on gardenia; (2) extracting the steam exploded fructus Gardeniae with ethanol under reflux, filtering, recovering ethanol from the filtrate, concentrating the recovered residual solution, and spray drying to obtain fructus Gardeniae pigment dry powder.
2. The steam explosion method for extracting gardenia pigment according to claim 1, which is characterized in that: the step (1) is that firstly, the gardenia is put into a steam explosion device, saturated steam is introduced inwards, the steam pressure is maintained at 3-10 Mpa, and the steam explosion is carried out for 2-6 min, so as to obtain the steam explosion gardenia.
3. The steam explosion method for extracting gardenia pigment according to claim 1, which is characterized in that: in the step (2), the extraction conditions of the gardenia subjected to steam explosion are that the concentration of ethanol for reflux is 70-80%, the ethanol is refluxed for 2 times, 20-30min is carried out each time, and filtered filtrates are combined and recovered until no alcohol smell exists.
4. An application method of gardenia pigment extracted by steam explosion in poultry breeding is characterized in that: the gardenia pigment as claimed in any one of claims 1 to 3 is added to daily ration of laying fowls as a feed additive to feed the laying fowls.
5. The method for applying gardenia pigment extracted by steam explosion in poultry farming according to claim 4, which is characterized in that: the laying fowl can be chicken, duck or goose.
6. The method for applying gardenia pigment extracted by steam explosion in poultry farming according to claim 5, which is characterized in that: the gardenia pigment is used as a feed additive and is added into daily ration according to the weight ratio of 0.05-0.1% in the early egg laying period and the egg laying period of laying fowls.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010587325.XA CN111675923B (en) | 2020-06-24 | 2020-06-24 | Method for extracting gardenia pigment through steam explosion and application method of extracted pigment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010587325.XA CN111675923B (en) | 2020-06-24 | 2020-06-24 | Method for extracting gardenia pigment through steam explosion and application method of extracted pigment |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN111675923A true CN111675923A (en) | 2020-09-18 |
| CN111675923B CN111675923B (en) | 2021-05-25 |
Family
ID=72456546
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010587325.XA Active CN111675923B (en) | 2020-06-24 | 2020-06-24 | Method for extracting gardenia pigment through steam explosion and application method of extracted pigment |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111675923B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108967955A (en) * | 2018-08-01 | 2018-12-11 | 山东农业大学 | The extracting method of melanoidin in a kind of blacking jujube |
Citations (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1670037A (en) * | 2005-02-24 | 2005-09-21 | 河南大学 | Complex utilization of Gardenia |
| CN101165104A (en) * | 2006-10-18 | 2008-04-23 | 大闽食品（漳州）有限公司 | Technique for preparing gardenia yellow pigment |
| CN101190255A (en) * | 2006-11-22 | 2008-06-04 | 中国科学院过程工程研究所 | Kudzu root total flavone extracting after steam-explosion process |
| CN101411466A (en) * | 2008-11-24 | 2009-04-22 | 浙江省中药研究所有限公司 | Method for preparing cape jasmine yellow pigment using cape jasmine as raw material |
| CN101607218A (en) * | 2009-07-17 | 2009-12-23 | 中国科学院过程工程研究所 | Corn steam explosion separates and endosperm multi-component co-production utilizes technology |
| CN101659794A (en) * | 2009-09-27 | 2010-03-03 | 广西山云生化科技有限公司 | New process for extracting high-purity gardenia yellow pigment by membrane separation and purification technology |
| CN102115611A (en) * | 2010-01-04 | 2011-07-06 | 中国科学院过程工程研究所 | Method for performing steam explosion on chestnut hulls and coproducing pigment, tanning extract and active carbon |
| CN102732049A (en) * | 2012-06-04 | 2012-10-17 | 华中科技大学 | Method for preparing and extracting carotenoid from microbial thalli |
| CN202822824U (en) * | 2012-08-30 | 2013-03-27 | 潜江市绿海宝生物技术有限公司 | Novel flash tank for producing gardenia yellow pigment |
| CN106543768A (en) * | 2016-09-27 | 2017-03-29 | 济南米铎碳新能源科技有限公司 | The method that stalk pigment is extracted using steam explosion technology |
| WO2017156744A1 (en) * | 2016-03-17 | 2017-09-21 | Dsm Ip Assets B.V. | New gardenia blue pigment, preparation and use thereof |
| CN107266931A (en) * | 2017-07-10 | 2017-10-20 | 桂林融通科技有限公司 | A kind of method for extracting peanut shell uranidin |
| CN108912731A (en) * | 2018-08-09 | 2018-11-30 | 界首市恒仁服饰有限公司 | A kind of preparation method of vegetable dyeing agent |
| CN209967720U (en) * | 2019-05-17 | 2020-01-21 | 山东省农业科学院农产品研究所 | Novel flash tank for producing gardenia yellow pigment |
| CN110903677A (en) * | 2019-12-13 | 2020-03-24 | 九江学院 | Method for simultaneously preparing gardenia yellow pigment and blue pigment |
-
2020
- 2020-06-24 CN CN202010587325.XA patent/CN111675923B/en active Active
Patent Citations (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1670037A (en) * | 2005-02-24 | 2005-09-21 | 河南大学 | Complex utilization of Gardenia |
| CN101165104A (en) * | 2006-10-18 | 2008-04-23 | 大闽食品（漳州）有限公司 | Technique for preparing gardenia yellow pigment |
| CN101190255A (en) * | 2006-11-22 | 2008-06-04 | 中国科学院过程工程研究所 | Kudzu root total flavone extracting after steam-explosion process |
| CN101411466A (en) * | 2008-11-24 | 2009-04-22 | 浙江省中药研究所有限公司 | Method for preparing cape jasmine yellow pigment using cape jasmine as raw material |
| CN101607218A (en) * | 2009-07-17 | 2009-12-23 | 中国科学院过程工程研究所 | Corn steam explosion separates and endosperm multi-component co-production utilizes technology |
| CN101659794A (en) * | 2009-09-27 | 2010-03-03 | 广西山云生化科技有限公司 | New process for extracting high-purity gardenia yellow pigment by membrane separation and purification technology |
| CN102115611A (en) * | 2010-01-04 | 2011-07-06 | 中国科学院过程工程研究所 | Method for performing steam explosion on chestnut hulls and coproducing pigment, tanning extract and active carbon |
| CN102732049A (en) * | 2012-06-04 | 2012-10-17 | 华中科技大学 | Method for preparing and extracting carotenoid from microbial thalli |
| CN202822824U (en) * | 2012-08-30 | 2013-03-27 | 潜江市绿海宝生物技术有限公司 | Novel flash tank for producing gardenia yellow pigment |
| WO2017156744A1 (en) * | 2016-03-17 | 2017-09-21 | Dsm Ip Assets B.V. | New gardenia blue pigment, preparation and use thereof |
| CN106543768A (en) * | 2016-09-27 | 2017-03-29 | 济南米铎碳新能源科技有限公司 | The method that stalk pigment is extracted using steam explosion technology |
| CN107266931A (en) * | 2017-07-10 | 2017-10-20 | 桂林融通科技有限公司 | A kind of method for extracting peanut shell uranidin |
| CN108912731A (en) * | 2018-08-09 | 2018-11-30 | 界首市恒仁服饰有限公司 | A kind of preparation method of vegetable dyeing agent |
| CN209967720U (en) * | 2019-05-17 | 2020-01-21 | 山东省农业科学院农产品研究所 | Novel flash tank for producing gardenia yellow pigment |
| CN110903677A (en) * | 2019-12-13 | 2020-03-24 | 九江学院 | Method for simultaneously preparing gardenia yellow pigment and blue pigment |
Non-Patent Citations (4)
| Title |
|---|
| 曾凡逵等: "栀子黄色素的研究现状 ", 《中国食品添加剂》 * |
| 汤兴俊等: "栀子色素研究进展 ", 《食品科学》 * |
| 辛莎等: "栀子黄色素提取与纯化研究进展 ", 《经济林研究》 * |
| 高彦祥等: "栀子黄色素提取与精制研究进展 ", 《中国食品添加剂》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108967955A (en) * | 2018-08-01 | 2018-12-11 | 山东农业大学 | The extracting method of melanoidin in a kind of blacking jujube |
| CN108967955B (en) * | 2018-08-01 | 2022-03-22 | 山东农业大学 | Method for extracting melanoidin from black red dates |
Also Published As
| Publication number | Publication date |
|---|---|
| CN111675923B (en) | 2021-05-25 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104068238A (en) | Extruded crucian feed and preparation method thereof | |
| CN110143903A (en) | A method of extracting utility from shrimp shell | |
| CN103494008A (en) | Forage for 30- to 90-day-old pigs and preparation method thereof | |
| CN111675923B (en) | Method for extracting gardenia pigment through steam explosion and application method of extracted pigment | |
| CN106318602A (en) | Processing method of high-quality camellia oil | |
| CN102344872A (en) | Method for preparing sweet potato yellow wine containing anthocyanidin | |
| CN106387476A (en) | Feed for preventing poultry Newcastle disease | |
| CN107455331B (en) | Production method of DHA-rich nutritional health-care eggs | |
| CN103875939A (en) | Composite pig feed capable of increasing appetite as well as preparation method thereof | |
| CN105935100A (en) | Chai-chicken feed mainly composed of sweet potato and preparation method thereof | |
| CN106260690A (en) | A kind of chicken feed | |
| CN105199921A (en) | Passion fruit wine and brewing method thereof | |
| CN106071270A (en) | A kind of dog grain improving immunity and preparation method thereof | |
| CN104026355A (en) | Environment-friendly pig feed containing Chinese medicinal herbs and preparation method thereof | |
| CN105746866A (en) | Dog food suitable for feeding small dogs | |
| CN107254351A (en) | Utilize the black efficient method for producing oil of tiger seed | |
| CN100999615A (en) | Pigment extracted from fish skin and preparation process thereof | |
| CN108740331B (en) | Eucalyptus leaf polyphenol feed additive for improving fish meat quality and preparation method thereof | |
| CN112385746A (en) | Green ecological medicinal pig feed and preparation method thereof | |
| KR0135673B1 (en) | Hen's egg contains astaxanthin | |
| CN105901387A (en) | Broiler chicken feed for guiding qi downward and helping digestion and preparation method thereof | |
| CN104938651A (en) | Walnut oil capable of promoting intelligence and tonifying brains | |
| CN105767483A (en) | Dog food suitable for feeding large dogs | |
| CN112106888A (en) | Chinese wolfberry fruit residue antibiotic-free feed additive and processing and feeding methods thereof | |
| CN108936052A (en) | A kind of egg feedstuff and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |