CN111629831A - 用于离散解链分析的方法和组合物 - Google Patents

用于离散解链分析的方法和组合物 Download PDF

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CN111629831A
CN111629831A CN201980009454.6A CN201980009454A CN111629831A CN 111629831 A CN111629831 A CN 111629831A CN 201980009454 A CN201980009454 A CN 201980009454A CN 111629831 A CN111629831 A CN 111629831A
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nucleic acid
signal
target
probe
predetermined
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Chinese (zh)
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道格拉斯·惠特曼
珍妮弗·贝尔尼尔
威廉·旺
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Luminex Corp
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Luminex Corp
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • C12Q1/6813—Hybridisation assays
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    • C12Q1/6823—Release of bound markers
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/30—Oligonucleotides characterised by their secondary structure
    • C12Q2525/301—Hairpin oligonucleotides
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    • C12Q2527/101—Temperature
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    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/107—Temperature of melting, i.e. Tm
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    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/113—PCR

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  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CN201980009454.6A 2018-01-22 2019-01-22 用于离散解链分析的方法和组合物 Pending CN111629831A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862620298P 2018-01-22 2018-01-22
US62/620,298 2018-01-22
PCT/US2019/014512 WO2019144107A1 (en) 2018-01-22 2019-01-22 Methods and compositions for discrete melt analysis

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CN111629831A true CN111629831A (zh) 2020-09-04

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US (2) US11390902B2 (enExample)
EP (1) EP3743211A4 (enExample)
JP (1) JP2021511056A (enExample)
KR (1) KR20200115552A (enExample)
CN (1) CN111629831A (enExample)
AU (1) AU2019209444A1 (enExample)
BR (1) BR112020014412A2 (enExample)
CA (1) CA3088250A1 (enExample)
WO (1) WO2019144107A1 (enExample)

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JP2021511056A (ja) * 2018-01-22 2021-05-06 ルミネックス コーポレーション 不連続融解分析のための方法および組成物
US11039089B2 (en) * 2019-03-27 2021-06-15 Indian Institute Of Technology Ropar Thermal imaging for identifying a defect in a material
AU2020395229A1 (en) * 2019-12-03 2022-06-23 Biofire Diagnostics, Llc System and method for identifying analytes in assay using normalized Tm values
WO2023023533A1 (en) 2021-08-19 2023-02-23 Luminex Corporation Digital amplification assay analysis method
US20250131748A1 (en) * 2021-09-14 2025-04-24 Combinati Incorporated Systems and methods for polymerase chain reaction quantification
US20230348957A1 (en) * 2021-12-07 2023-11-02 Luminex Corporation Methods and compositions for nucleic acid analysis
KR102750761B1 (ko) * 2021-12-29 2025-01-09 주식회사 한국인삼공사 배암차즈기를 판별하기 위한 단일염기다형성을 갖는 유전자 마커
WO2023235732A2 (en) * 2022-05-31 2023-12-07 Siemens Healthcare Diagnostics Inc. Methods and kits for determining antibiotic susceptibility
KR20260011156A (ko) * 2023-05-12 2026-01-22 라이프 테크놀로지스 코포레이션 다중 중합효소 연쇄반응 공정 및 데이터 분석을 위한 시스템 및 방법
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CN105765583A (zh) * 2013-08-30 2016-07-13 犹他大学研究基金会 去除dna解链分析中的荧光背景的量子方法
CN106715720A (zh) * 2014-08-11 2017-05-24 卢米耐克斯公司 用于在核酸测定中改善解链分辨和多重性的探针
CN107208145A (zh) * 2014-12-30 2017-09-26 端粒诊断公司 多重定量pcr
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US20050053950A1 (en) * 2003-09-08 2005-03-10 Enrique Zudaire Ubani Protocol and software for multiplex real-time PCR quantification based on the different melting temperatures of amplicons
US20130095479A1 (en) * 2004-10-18 2013-04-18 Brandeis University Primers, probes and methods for nucleic acid amplification
CN102112632A (zh) * 2008-08-08 2011-06-29 史密斯探测公司 用于pcr分析的检测算法
EP2228454A1 (en) * 2009-03-10 2010-09-15 Roche Diagnostics GmbH Multiplex quantitative nucleic acid amplification and melting assay
CN102428459A (zh) * 2009-05-15 2012-04-25 爱达荷科技公司 用于熔化曲线自动分析的系统和方法
CN105765583A (zh) * 2013-08-30 2016-07-13 犹他大学研究基金会 去除dna解链分析中的荧光背景的量子方法
CN106715720A (zh) * 2014-08-11 2017-05-24 卢米耐克斯公司 用于在核酸测定中改善解链分辨和多重性的探针
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CN108368547A (zh) * 2015-12-15 2018-08-03 Seegene株式会社 与靶核酸序列有关的信号提取

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BR112020014412A2 (pt) 2020-12-29
WO2019144107A1 (en) 2019-07-25
JP2021511056A (ja) 2021-05-06
CA3088250A1 (en) 2019-07-25
EP3743211A1 (en) 2020-12-02
US11390902B2 (en) 2022-07-19
US20190226008A1 (en) 2019-07-25
AU2019209444A1 (en) 2020-07-30
KR20200115552A (ko) 2020-10-07
EP3743211A4 (en) 2021-11-24
US20220364148A1 (en) 2022-11-17

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