CA3088250A1 - Methods and compositions for discrete melt analysis - Google Patents
Methods and compositions for discrete melt analysis Download PDFInfo
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- CA3088250A1 CA3088250A1 CA3088250A CA3088250A CA3088250A1 CA 3088250 A1 CA3088250 A1 CA 3088250A1 CA 3088250 A CA3088250 A CA 3088250A CA 3088250 A CA3088250 A CA 3088250A CA 3088250 A1 CA3088250 A1 CA 3088250A1
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2527/00—Reactions demanding special reaction conditions
- C12Q2527/143—Concentration of primer or probe
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2531/00—Reactions of nucleic acids characterised by
- C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
- C12Q2531/113—PCR
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| PCT/US2019/014512 WO2019144107A1 (en) | 2018-01-22 | 2019-01-22 | Methods and compositions for discrete melt analysis |
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| KR20200115552A (ko) * | 2018-01-22 | 2020-10-07 | 루미넥스 코포레이션 | 별개의 용융 분석을 위한 방법 및 조성물 |
| US11039089B2 (en) * | 2019-03-27 | 2021-06-15 | Indian Institute Of Technology Ropar | Thermal imaging for identifying a defect in a material |
| AU2020395229B2 (en) * | 2019-12-03 | 2026-07-16 | Biofire Diagnostics, Llc | System and method for identifying analytes in assay using normalized Tm values |
| WO2023023533A1 (en) | 2021-08-19 | 2023-02-23 | Luminex Corporation | Digital amplification assay analysis method |
| US20250131748A1 (en) * | 2021-09-14 | 2025-04-24 | Combinati Incorporated | Systems and methods for polymerase chain reaction quantification |
| CA3232383C (en) * | 2021-09-30 | 2026-05-12 | Gen-Probe Incorporated | Temperature-selectable fret cassette signaling |
| WO2023108014A1 (en) * | 2021-12-07 | 2023-06-15 | Luminex Corporation | Methods and compositions for nucleic acid analysis |
| KR102750761B1 (ko) * | 2021-12-29 | 2025-01-09 | 주식회사 한국인삼공사 | 배암차즈기를 판별하기 위한 단일염기다형성을 갖는 유전자 마커 |
| JP2025518761A (ja) * | 2022-05-31 | 2025-06-19 | シーメンス・ヘルスケア・ダイアグノスティックス・インコーポレーテッド | 抗菌薬感受性を測定する方法及びキット |
| CN121335987A (zh) * | 2023-05-12 | 2026-01-13 | 生命科技股份有限公司 | 用于多重聚合酶链式反应过程和数据分析的系统和方法 |
| WO2025179142A1 (en) * | 2024-02-22 | 2025-08-28 | Cepheid | Maximizing optical channel capabilities for target detection |
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| US5200313A (en) * | 1983-08-05 | 1993-04-06 | Miles Inc. | Nucleic acid hybridization assay employing detectable anti-hybrid antibodies |
| JP3963422B2 (ja) * | 2000-08-03 | 2007-08-22 | 日鉄環境エンジニアリング株式会社 | 核酸の測定方法 |
| US7058184B1 (en) | 2003-03-25 | 2006-06-06 | Robert Hickling | Acoustic measurement method and apparatus |
| US20050053950A1 (en) * | 2003-09-08 | 2005-03-10 | Enrique Zudaire Ubani | Protocol and software for multiplex real-time PCR quantification based on the different melting temperatures of amplicons |
| WO2006044994A2 (en) * | 2004-10-18 | 2006-04-27 | Brandeis University | Primers, probes and methods for nucleic acid amplification |
| EP2140033B1 (en) * | 2007-03-28 | 2012-10-10 | Signal Diagnostics | System and method for high resolution analysis of nucleic acids to detect sequence variations |
| WO2009158451A1 (en) * | 2008-06-25 | 2009-12-30 | Real-Time Genomics, Llc | Method and apparatus for melting curve analysis of nucleic acids in microarray format |
| US20110151461A1 (en) | 2008-08-08 | 2011-06-23 | Smiths Detection-Edgewood Inc. | Detection algorithm for pcr assay |
| US9156010B2 (en) | 2008-09-23 | 2015-10-13 | Bio-Rad Laboratories, Inc. | Droplet-based assay system |
| US9417190B2 (en) | 2008-09-23 | 2016-08-16 | Bio-Rad Laboratories, Inc. | Calibrations and controls for droplet-based assays |
| AU2009324806A1 (en) * | 2008-12-10 | 2011-07-07 | Smiths Detection Inc. | Identification and differentiation of nucleic acid sequence using temperature-dependent hybridization |
| US8039215B2 (en) * | 2009-03-10 | 2011-10-18 | Roche Molecular Systems, Inc. | Multiplex quantitative nucleic acid amplification and melting assay |
| CA2756300C (en) * | 2009-05-15 | 2017-11-14 | Idaho Technology, Inc. | Systems and methods for automated melting curve analysis |
| GB201017978D0 (en) * | 2010-10-25 | 2010-12-08 | Oxitec Ltd | Multiplex amplification and detection |
| US9169514B2 (en) * | 2010-12-03 | 2015-10-27 | Brandeis University | Detecting nucleic acid variations within populations of genomes |
| MX352460B (es) * | 2011-01-11 | 2017-11-24 | Seegene Inc | Detección de secuencias de ácido nucleico objetivo mediante ensayo de escisión y extensión del pto. |
| EP2885431A4 (en) * | 2012-08-17 | 2016-07-13 | Bio Nems Corp | CLASSIFICATION OF A NUCLEIC ACID |
| WO2015023616A2 (en) | 2013-08-12 | 2015-02-19 | The Johns Hopkins University | Method for comprehensive, quantitative, and highly sensitive discrimination of nucleic acid sequences in homogeneous and heterogeneous populations |
| CN105765583B (zh) * | 2013-08-30 | 2020-01-24 | 犹他大学研究基金会 | 去除dna解链分析中的荧光背景的量子方法 |
| WO2015147370A1 (en) * | 2014-03-28 | 2015-10-01 | Seegene, Inc. | Detection of target nucleic acid sequences using different detection temperatures |
| EP3180449B1 (en) * | 2014-08-11 | 2019-10-09 | Luminex Corporation | Probes for improved melt discrimination and multiplexing in nucleic acid assays |
| CN107109492B (zh) * | 2014-12-22 | 2021-02-26 | 阿纳帕生物技术股份公司 | 用于靶核酸多重检测的双重猝灭测定 |
| WO2016108954A1 (en) * | 2014-12-30 | 2016-07-07 | Telomere Diagnostics, Inc. | Multiplex quantitative pcr |
| RS60032B1 (sr) * | 2015-05-18 | 2020-04-30 | Saga Diagnostics Ab | Detekcija ciljne nukleinske kiseline i varijanti |
| EP3356555B1 (en) | 2015-09-29 | 2021-07-07 | Life Technologies Corporation | Systems and methods for performing digital pcr |
| CN108368547B (zh) * | 2015-12-15 | 2022-04-05 | Seegene株式会社 | 与靶核酸序列有关的信号提取 |
| US11232851B2 (en) | 2016-06-23 | 2022-01-25 | Canon Information And Imaging Solutions, Inc. | System and method for modeling and subtracting background signals from a melt curve |
| DK3512959T3 (da) | 2016-09-15 | 2021-10-18 | Hoffmann La Roche | Fremgangsmåder til udførelse af multipleks PCR |
| EP3299470B1 (en) | 2016-09-23 | 2019-10-30 | Roche Diagniostics GmbH | A method for reducing quantification errors caused by reaction volume deviations in digital polymerase chain reaction |
| EP3984642B1 (en) | 2016-11-17 | 2023-09-06 | Combinati Incorporated | Methods and systems for nucleic acid analysis and quantification |
| KR20200115552A (ko) * | 2018-01-22 | 2020-10-07 | 루미넥스 코포레이션 | 별개의 용융 분석을 위한 방법 및 조성물 |
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| US11390902B2 (en) | 2022-07-19 |
| EP3743211A1 (en) | 2020-12-02 |
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| US20190226008A1 (en) | 2019-07-25 |
| US20220364148A1 (en) | 2022-11-17 |
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| KR20200115552A (ko) | 2020-10-07 |
| EP3743211A4 (en) | 2021-11-24 |
| AU2019209444A1 (en) | 2020-07-30 |
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