CN111617144B

CN111617144B - Quick-sterilizing pharmaceutical composition and preparation method thereof

Info

Publication number: CN111617144B
Application number: CN202010607461.0A
Authority: CN
Inventors: 李福元; 薛兴华; 王顺山; 严永; 郭智伟; 程安达; 王心喜; 江厚生
Original assignee: Beijing Centre Biology Co ltd
Current assignee: Beijing Centre Biology Co ltd
Priority date: 2020-06-29
Filing date: 2020-06-29
Publication date: 2022-03-11
Anticipated expiration: 2040-06-29
Also published as: CN111617144A

Abstract

The invention provides a quick sterilization pharmaceutical composition and a preparation method thereof. The composition is prepared from the following raw materials in parts by weight: 1-6 parts of dark plum, 6-60 parts of water, 1-1.5 parts of thyme, 6-12 parts of 30-40% ethanol and 0.3-0.6 part of emulsifier, wherein the preparation method comprises the following steps: taking 1-6 parts of dark plum medicinal materials by weight, putting the dark plum medicinal materials into the lower part of an extraction tank, adding 6-60 parts of water, and decocting and extracting the raw materials; taking 1-1.5 parts by weight of thyme crushed into 50-100 meshes and 6-12 parts of 30-40% ethanol, putting the thyme and the ethanol into the upper part of an extraction tank, stirring the solution in the extraction process, and extracting for 2 times, wherein each time lasts for 1.5 hours; and discharging the solution extracted by the S2, adding 0.3-0.6 part of emulsifier, uniformly mixing for 1.5h, concentrating the mixed extracting solution and the solution extracted by the S1 respectively, and fully mixing to obtain the pharmaceutical composition. The invention has the advantages of high sterilization speed, obvious sterilization effect, simultaneous extraction of effective components and energy consumption saving.

Description

Quick-sterilizing pharmaceutical composition and preparation method thereof

Technical Field

The invention relates to the technical field of bactericidal medicine compositions, in particular to a quick bactericidal medicine composition and a preparation method thereof.

Background

Mastitis is one of the common diseases of dairy cows, and is the inflammation of mammary parenchyma and interstitial tissue caused by invasion of pathogenic microorganisms into breasts or other stress factors. Because it damages mammary tissue to influence lactation function, the milk yield is reduced, and in severe cases, sick cows can be eliminated, deteriorated milk can harm human health, and great economic loss is brought to the dairy cow industry.

More than one hundred pathogenic microorganisms causing mastitis are commonly seen, such as streptococcus, staphylococcus, corynebacterium pyogenes and the like, wherein the streptococcus agalactiae accounts for more than 50% of pathogens causing mastitis, the staphylococcus aureus accounts for 30%, and the corynebacterium pyogenes accounts for about 20%. Teat dipping with an antiseptic solution immediately after each milking is an effective method to reduce the incidence of mastitis. The medicated bath can kill pathogenic pathogens on the teat, inhibit proliferation of pathogens in the teat canal, and also help recovery from teat pain, prevent new wound formation, and provide a protective bactericidal environment for the teat between milkings. An effective nipple bath lotion which, when used properly, reduces new breast infections by approximately 50% to 90%.

The medicated bath of the nipples is carried out after the milking of the cows, so that the invasion of pathogenic microorganisms to the breasts can be reduced, and the clinical incidence rate of mastitis is reduced. The conventional nipple medicated bath liquid has various types, but some medicated bath liquids have certain damage and stimulation to the skin and internal tissues of the mammary gland of the dairy cow. The currently applied medicated bath solution for the nipples of the dairy cows is mainly an iodine preparation which is generally composed of polyvinylpyrrolidone (PVP), iodine, potassium iodide, glycerol and the like. The medicated bath liquid can form a protective film on the nipple of milk cow, and has certain protective effect on the nipple. But because of the instability of chemical components, the quality of the product is easy to be uncontrollable, and certain damage can be caused to the nipple skin of the dairy cow after long-term use; and usually, the raw material extraction of the medicated bath solution is realized by independently extracting various raw materials, a plurality of extraction devices are required to be arranged for independent extraction, so that the production process is increased, time and labor are wasted, a large amount of electric energy is consumed by the extraction devices, a lot of resources are wasted, and the production cost is improved.

Therefore, there is a need to provide a new pharmaceutical composition with rapid sterilization and a preparation method thereof to solve the above technical problems.

Disclosure of Invention

The invention provides a pharmaceutical composition for rapid sterilization, which has the advantages of rapid sterilization speed, remarkable sterilization effect, simultaneous extraction of effective components, stirring driven by steam and energy consumption saving, and a preparation method thereof.

A quick-sterilizing pharmaceutical composition is prepared from the following raw materials in parts by weight: 1-6 parts of dark plum, 6-60 parts of water, 1-1.5 parts of thyme, 6-12 parts of 30-40% ethanol and 0.3-0.6 part of emulsifier.

A method for producing a rapid bactericidal pharmaceutical composition, comprising the steps of:

s1: taking 1-6 parts of dark plum medicinal materials according to the weight part, putting the dark plum medicinal materials into the lower part of an extraction tank, adding 6-60 parts of water, decocting and extracting the raw materials twice, extracting for 3 hours each time, and concentrating to obtain the crude drug content of 0.05-2 g/mL;

s2: taking 1-1.5 parts by weight of thyme crushed into 50-100 meshes and 6-12 parts by weight of 30-40% ethanol, putting the thyme and the ethanol into the upper part of an extraction tank, heating the extraction tank by using water vapor generated by decoction in S1, regulating the temperature by controlling a valve to control the temperature of the upper part of the extraction tank to be 50-75 ℃, stirring the solution in the extraction process, and extracting for 2 times, wherein each time lasts for 1.5 hours;

s3, discharging the solution extracted in the S2, adding 0.3-0.6 part of emulsifier, mixing uniformly for 1.5h, and concentrating to obtain the crude drug content of 0.1-5 g/mL;

s4, fully mixing the concentrated liquid obtained from S1 and S3 to obtain a pharmaceutical composition; wherein the content of the first and second substances,

the extraction tank in the above includes:

a tank body; the channel structure is arranged on the side wall of the tank body; the device comprises a communicating structure, a first switch, a steam pipe, an upper discharging pipe, a lower discharging pipe and two filter screens, wherein the communicating structure is arranged on the side wall of the tank body and comprises a communicating pipe, the first switch, the steam pipe, the upper discharging pipe, the lower discharging pipe and the filter screens; the mixing structure is communicated with the bottom end of the communicating pipe and comprises a mixing barrel, the interior of the mixing barrel is communicated with the bottom end of the communicating pipe, and the bottom end of the upper discharging pipe is communicated with the interior of the mixing barrel; the second partition plate is fixed on the inner wall of the tank body; the ventilation structure is arranged inside the second partition plate and comprises an air outlet pipe, a ball valve and a warming cavity, the air outlet pipe is arranged inside the second partition plate, one end of the air outlet pipe is positioned inside the top end of the communicating pipe, the ball valve is rotatably connected inside the air outlet pipe, the warming cavity is arranged on the inner wall of the upper part of the tank body, and the warming cavity is communicated with the air outlet pipe and the vent hole; the stirring structure is rotatably connected to the inside of the tank body and comprises a first rotating shaft, first rotating blades, first stirring blades and second rotating blades, two ends of the first rotating shaft are rotatably connected to the top end and the bottom end of the inside of the tank body, the first rotating blades are fixed to the middle of the first rotating shaft and rotate inside the air outlet pipe, the two first stirring blades are respectively fixed to the upper portion and the lower portion of the first rotating shaft, the second rotating blades are fixed to the top end of the first rotating shaft, and the second rotating blades rotate inside the temperature rising cavity; the linkage structure is rotatably connected inside the communicating pipe and comprises a second rotating shaft, a second stirring blade and a third rotating blade, the second rotating shaft is rotatably connected inside the communicating pipe and the mixing barrel, the second stirring blade is fixed at the bottom end of the second rotating shaft, the upper part of the inside of the second stirring blade is fixed at the upper part of the second rotating shaft, and the third rotating blade is rotatably connected inside the air outlet pipe; and the one-way valve is arranged inside the second partition plate and inside the warming cavity.

Preferably, the channel structure includes first feed inlet, second feed inlet, blow vent, first relief valve and second relief valve, first feed inlet with the second feed inlet is located the lateral wall of the jar body, just first feed inlet with the second feed inlet with the lower part and the upper portion of the internal portion of jar are linked together, the blow vent is located the top surface of the jar body, first relief valve with the second relief valve is located the lateral wall of the jar body, just first relief valve with the chamber intercommunication heaies up, the second relief valve with the lower part of the internal portion of jar communicates.

Preferably, mixed structure still includes discharge gate, second switch, interpolation mouth, first baffle and intercommunication mouth, the discharge gate is located the lateral wall bottom of blending bin, just the discharge gate with the inside lower part intercommunication of blending bin, the second switch rotate connect in the inside of blending bin, it locates to add the mouth the lateral wall upper end of blending bin, just add the mouth with the inside upper portion intercommunication of blending bin, first baffle is fixed in the inner wall of blending bin, intercommunication mouth intercommunication with the inside upper and lower part of blending bin, just the second switch is contradicted the intercommunication mouth.

Preferably, the interlock structure still includes first helical gear, second helical gear, dwang and cleaning brush, first helical gear is fixed in the top of second pivot, the second helical gear with first helical gear meshing is connected, the one end of dwang is fixed in the lateral wall of second helical gear, just the dwang rotate connect in the inside of filter screen, the cleaning brush is fixed in the other end of dwang, just the cleaning brush is contradicted the lateral wall of filter screen.

Preferably, a heating plate is installed on the bottom surface of the interior of the tank body.

Compared with the related technology, the rapid sterilization pharmaceutical composition and the preparation method thereof provided by the invention have the following beneficial effects:

(1) the invention provides a pharmaceutical composition for rapid sterilization and a preparation method thereof, wherein a dark plum extract has an inhibitory effect on escherichia coli, shigella dysenteriae, typhoid bacillus, paratyphoid bacillus, cholera bacillus, pertussis bacillus, proteus bacillus, bacillus anthracis, diphtheria bacillus, diphtheroid bacillus, human mycobacterium tuberculosis, meningococcus, staphylococcus aureus, pneumococcus, hemolytic streptococcus and the like in vitro; the thyme contains volatile oil, flavonoid, organic acid, amino acid and trace elements, is a natural medicinal plant with antifungal, antibacterial and pesticide effects, and contains thymol, carvacrol, linalool and geraniol as main ingredients. Through the verification of the graded bacteriostasis concentration index of the selected traditional Chinese medicines with the antibacterial effect, after the dark plum water extract and the thyme alcohol extract are jointly used, the sterilization speed and the sterilization effect are obviously improved, and the mixed extract of the two materials does not contain components such as polyvinyl pyrrolidone (PVP), iodine, potassium iodide, glycerol and the like, so that the stimulation to the nipples of the dairy cows is almost avoided, and the nipples of the dairy cows are not damaged.

(2) The equipment of the invention simultaneously performs a dark plum extraction method and a thyme extraction method, and can heat thyme extraction by using steam generated in the dark plum extraction process, the generated steam is recycled, a stirring structure is driven by the steam to stir an extraction solution, so that an emulsifier is fully mixed with thyme extracting solution, and finally the mixed thyme extracting solution is mixed with the dark plum extracting solution, thereby greatly saving time, accelerating the preparation efficiency and saving energy consumption.

Drawings

FIG. 1 is a flow chart of a rapid sterilization pharmaceutical composition and a method for preparing the same according to the present invention;

FIG. 2 is a schematic view of the overall structure of the extraction tank of the present invention;

FIG. 3 is a schematic cross-sectional view taken at A-A of the extraction tank shown in FIG. 2;

FIG. 4 is an enlarged schematic view of area A shown in FIG. 3;

FIG. 5 is a schematic view of the first bevel gear shown in FIG. 4;

FIG. 6 is a schematic structural view of the third rotary vane shown in FIG. 3;

FIG. 7 is an enlarged schematic view of region B shown in FIG. 3;

fig. 8 is an enlarged schematic view of the region C shown in fig. 3.

Reference numbers in the figures: 100. an extraction tank 1, a tank body 2, a channel structure 21, a first inlet port 22, a second inlet port 23, an air vent 24, a first pressure relief valve 25, a second pressure relief valve 3, a communicating structure 31, a communicating pipe 32, a first switch 33, a steam pipe 34, an upper discharge pipe 35, a lower discharge pipe 36, a filter screen 4, a mixing structure 41, a mixing barrel 42, a discharge port 43, a second switch 44, an adding port 45, a first partition plate 46, a communicating port 5, a second partition plate 6, an air vent structure 61, an air outlet pipe 62, a ball valve 63, a warming cavity 7, a stirring structure 71, a first rotating shaft 72, a first rotating blade 73, a first stirring blade 74, a second rotating blade 8, a linkage structure 81, a second rotating shaft 82, a second stirring blade 83, a third rotating blade 84, a first rotating shaft 85, a second rotating shaft, 86. rotating rod 87, cleaning brush 9, one-way valve 9a and heating plate.

Detailed Description

The invention is further described with reference to the following figures and embodiments.

Referring to fig. 1, fig. 2, fig. 3, fig. 4, fig. 5, fig. 6, fig. 7 and fig. 8, a fast bactericidal pharmaceutical composition is prepared from the following raw materials in parts by weight: 1-6 parts of dark plum, 6-60 parts of water, 1-1.5 parts of thyme, 6-12 parts of food grade ethanol with the concentration of 30% -40% and 0.3-0.6 part of emulsifier.

s1: taking 1-6 parts of dark plum medicinal materials according to the weight part, putting the dark plum medicinal materials into the lower part of an extraction tank 100, adding 6-60 parts of water, decocting and extracting the raw materials twice, extracting for 3 hours each time, and concentrating to obtain the crude drug content of 0.05-2 g/mL;

s2: taking 1-1.5 parts by weight of thyme crushed into 50-100 meshes and 6-12 parts by weight of 30-40% food grade ethanol, putting the thyme and the food grade ethanol into the upper part of an extraction tank 100, heating the extraction tank by using water vapor generated by decoction in S1, regulating the temperature by controlling a valve, controlling the temperature of the upper part of the extraction tank 100 to be 50-75 ℃, stirring the solution in the extraction process, and extracting for 2 times, wherein each time lasts for 1.5 hours;

s4, fully mixing the concentrated liquid obtained from S1 and S3 to obtain a pharmaceutical composition;

wherein the content of the first and second substances,

the extraction tank 100 in the above includes: a tank body 1; the channel structure 2 is arranged on the side wall of the tank body 1; the communicating structure 3 is arranged on the side wall of the tank body 1, the communicating structure 3 comprises a communicating pipe 31, a first switch 32, a steam pipe 33, an upper discharging pipe 34, a lower discharging pipe 35 and filter screens 36, the top end of the communicating pipe 31 is arranged on the side wall of the tank body 1, the first switch 32 is rotatably connected inside the communicating pipe 31, the steam pipe 33 is fixed on the top end of the communicating pipe 31, the upper discharging pipe 34 is arranged inside the communicating pipe 31, the top end of the upper discharging pipe 34 is communicated with the upper part inside the tank body 1, one end of the lower discharging pipe 35 is communicated with the lower part inside the tank body 1, the other end of the lower discharging pipe 35 is communicated with the upper discharging pipe 34, and the two filter screens 36 are respectively arranged inside the top end of the upper discharging pipe 34 and inside the lower discharging pipe 35; the mixing structure 4 is communicated with the bottom end of the communication pipe 31, the mixing structure 4 comprises a mixing barrel 41, the interior of the mixing barrel 41 is communicated with the bottom end of the communication pipe 31, and the bottom end of the upper discharge pipe 34 is communicated with the interior of the mixing barrel 41; the second partition plate 5 is fixed on the inner wall of the tank body 1; the ventilation structure 6 is arranged inside the second partition plate 5, the ventilation structure 6 comprises an air outlet pipe 61, a ball valve 62 and a warming cavity 63, the air outlet pipe 61 is arranged inside the second partition plate 5, one end of the air outlet pipe 61 is positioned inside the top end of the communicating pipe 31, the ball valve 62 is rotatably connected inside the air outlet pipe 61, the warming cavity 63 is arranged on the inner wall of the upper part of the tank body 1, and the warming cavity 63 is communicated with the air outlet pipe 61 and the vent 23; the stirring structure 7 is rotatably connected inside the tank body 1, the stirring structure 7 includes a first rotating shaft 71, a first rotating blade 72, a first stirring blade 73 and a second rotating blade 74, two ends of the first rotating shaft 71 are rotatably connected to the top end and the bottom end inside the tank body 1, the first rotating blade 72 is fixed in the middle of the first rotating shaft 71, the first rotating blade 72 rotates inside the air outlet pipe 61, the two first stirring blades 73 are respectively fixed on the upper part and the lower part of the first rotating shaft 71, the second rotating blade 74 is fixed at the top end of the first rotating shaft 71, and the second rotating blade 74 rotates inside the warming cavity 63; the linkage structure 8, the linkage structure 8 is rotatably connected to the inside of the communication pipe 31, the linkage structure 8 includes a second rotating shaft 81, a second stirring blade 82 and a third rotating blade 83, the second rotating shaft 81 is rotatably connected to the inside of the communication pipe 31 and the mixing barrel 41, the second stirring blade 82 is fixed at the bottom end of the second rotating shaft 81, the upper part of the inside of the second stirring blade 82 is fixed, the third rotating blade 83 is fixed at the upper part of the second rotating shaft 81, and the third rotating blade 83 is rotatably connected to the inside of the air outlet pipe 61; and a check valve 9, wherein the check valve 9 is installed inside the second partition plate 5 and inside the warming cavity 63.

Specifically, channel structure 2 includes first feed inlet 21, second feed inlet 22, blow vent 23, first relief valve 24 and second relief valve 25, first feed inlet 21 with second feed inlet 22 is located the lateral wall of the jar body 1, just first feed inlet 21 with second feed inlet 22 with the lower part and the upper portion of the internal portion of jar body 1 are mutually linked, blow vent 23 is located the top surface of the jar body 1, first relief valve 24 with second relief valve 25 is located the lateral wall of the jar body 1, just first relief valve 24 with the chamber 63 that heats communicates, second relief valve 25 with the lower part of the internal portion of the jar body 1 communicates, in order to put into various raw and other materials the internal and reduction of jar pressure in the jar body 1.

Specifically, mixed structure 4 still includes discharge gate 42, second switch 43, adds mouth 44, first baffle 45 and intercommunication mouth 46, discharge gate 42 is located the lateral wall bottom of blending bin 41, just discharge gate 42 with the inside lower part intercommunication of blending bin 41, second switch 43 rotate connect in the inside of blending bin 41, it locates to add mouth 44 the lateral wall upper end of blending bin 41, just add mouth 44 with the inside upper portion intercommunication of blending bin 41, first baffle 45 is fixed in the inner wall of blending bin 41, intercommunication mouth 46 communicate with the inside upper and lower part of blending bin 41, just second switch 43 is contradicted intercommunication mouth 46, in order to incite somebody to action blending bin 41 is inside to be divided into two independent spaces, makes things convenient for the mixture and the storage of extract.

Specifically, the linkage structure 8 further comprises a first bevel gear 84, a second bevel gear 85, a rotating rod 86 and a cleaning brush 87, the first bevel gear 84 is fixed at the top end of the second rotating shaft 81, the second bevel gear 85 is engaged with the first bevel gear 84, one end of the rotating rod 86 is fixed at the side wall of the second bevel gear 85, the rotating rod 86 is rotatably connected to the inside of the filter screen 36, the cleaning brush 87 is fixed to the other end of the rotating rod 86, and the cleaning brush 87 abuts against the side wall of the filter screen 36, in order that when the second rotating shaft 81 rotates, the first bevel gear 84 can be driven to rotate, the first bevel gear 84 drives the second bevel gear 85 to rotate, and then the rotating rod 86 and the cleaning brush 87 are driven to rotate, so that the filter screen 36 at the top end of the upper discharge pipe 34 is cleaned and kept clean.

Specifically, a heating plate 9a is installed on the inner bottom surface of the can body 1 to heat the dark plum and water, thereby producing the dark plum extract.

The preparation process of the bactericidal pharmaceutical composition provided by the invention comprises the following steps:

switching on an external power supply, wherein in the preparation process, 1-6 parts by weight of dark plum medicinal materials are put into the lower part of the tank body 1 through the first feeding hole 21, 6-60 parts by weight of water is added, the heating plate 9a is opened to decoct and extract the raw materials, the extraction is carried out twice, 3 hours each time, and the extracted solution is discharged into the lower part in the mixing barrel 41 through the lower discharging pipe 35; then, 1-1.5 parts by weight of thyme crushed into 50-100 meshes and 6-12 parts by weight of 30% -40% ethanol are put into the upper part in the tank body 1 through the second feed port 22, the water vapor generated by heating the lower part of the tank body 1 is utilized to heat the upper part in the tank body 1 to 50-75 ℃, and the solution is stirred through the stirring structure 7 for 2 times of extraction. 1.5 hours each time; then the mixture is discharged into the upper part of the interior of the mixing barrel 41 through the upper discharge pipe 34, then 0.3-0.6 part of emulsifier is added, the mixture is stirred by the second stirring blade 82, and the mixture is discharged into the lower part of the interior of the mixing barrel 41 after being fully stirred; finally, taking out the thyme extract and the dark plum extract and respectively concentrating to respectively obtain the crude drug content of 0.1g-5g/mL and 0.05g-2 g/mL; the two are mixed well.

Specifically, firstly, the first feeding hole 21 and the second feeding hole 22 are opened, 1 to 6 parts of dark plum and 6 to 60 parts of water are placed in the inner lower part of the tank body 1, 1 to 1.5 parts of thyme and 6 to 12 parts of 30 to 40 percent ethanol are placed in the inner upper part of the tank body 1, the first feeding hole 21 and the second feeding hole 22 are closed, the heating plate 9a is opened, the ball valve 62 is adjusted at the same time, so that the air outlet pipe 61 is communicated with the warming cavity 63, the heating plate 9a continuously heats the raw material in the lower part of the tank body 1, the generated steam pushes open the check valve 9 in the second partition plate 6 after reaching a certain amount, so that the inner lower part of the tank body 1 is communicated with the air outlet pipe 61, the steam passes through the narrow passage of the check valve 9, and the steam spraying speed is increased, steam gets into during the outlet duct 61, can drive first rotating vane 72 rotates, first rotating vane 72 drives first pivot 71 rotates, first pivot 71 drives first stirring leaf 73 rotates, it is right raw materials in the jar body 1 stir, later steam passes through outlet duct 61 enters into in the chamber 63 heats, the user rotates according to the temperature instruction ball valve 62, change ball valve 62 with the size of the intercommunication pipeline between the chamber 63 heats, adjust the volume that gets into the steam in the chamber 63 heats, thereby control the temperature in the chamber 63 heats, the chamber 63 temperature that heats rises, and then will the upper space heating in the jar body 1 for the reaction temperature of thyme and ethanol reaches 50-75 degrees centigrade, when steam in the chamber 61 that heats reaches certain pressure after, will push open the check valve 9 of the chamber 61 top surface that heats, blowing the steam to the second rotating blade 74 to drive the second rotating blade 74 to rotate, so that the first stirring blade 73 rotates more stably, the stirring effect is better, and meanwhile, the rising steam is discharged out of the temperature rising cavity 63 through the vent 23; because the reaction time of the thyme is short, the partial raw materials on the extracting solution 1 are firstly extracted, after the extraction is completed, the ball valve 62 is rotated to disconnect the outlet pipe 61 from the warming cavity 63 and communicate with the communicating pipe 31, the first pressure release valve 24 and the upper discharge pipe 34 are opened at the same time, the steam in the warming cavity 63 is rapidly discharged, the temperature and the pressure of the upper part in the tank body 1 are rapidly reduced, the extracting solution is discharged from the upper discharge pipe 34, filtered by the filter screen 36, the thyme extracting solution flows into the upper part in the mixing barrel 41, after the thyme extracting solution completely flows into the upper part in the mixing barrel 41, the upper discharge pipe 34 is closed, meanwhile, the rapidly flowing steam enters the top end inside the communicating pipe 31 to drive the third rotating blade 83 to rotate, and then the steam is discharged through the steam pipe 33, third rotating leaf 83 drives second pivot 81 rotates, second pivot 81 drives second stirring leaf 82 with first helical gear 84 rotates, first helical gear 84 drives second helical gear 85 rotates, second helical gear 85 drives dwang 86 rotates, dwang 86 drives clearance brush 87 rotates, clearance brush 87 sweeps 36 surfaces of filter screen, clears up thyme residue, keeps filter screen 36 surfaces clean, opens this moment add mouthful 44, puts into 1-2 parts of emulsifier (emulsifier is one of tween-80, hydrogenated castor oil, fatty alcohol polyoxyethylene ether sodium sulfate), second stirring leaf 82 stirs the thyme extract of adding the emulsifier, after the intensive mixing, opens second switch 43, intercommunication mouth 46 will space intercommunication about in the mixing barrel 41, the upper part of the extracting solution enters the lower part through the communicating port 46 to be temporarily stored, the second switch 43 is closed, after the extraction of the dark plum raw material is finished, the heating plate 9a is closed, the second pressure release valve 25 is opened, the steam at the lower part in the tank body 1 is discharged, the pressure is reduced, then the first switch 32 is opened, the dark plum extracting solution enters the lower discharging pipe 35 and is filtered through the filter screen 36, the dark plum extracting solution enters the upper space of the mixing barrel 41, and the dark plum extracting solution is taken out to be separately stored. Close first relief valve 24 with second relief valve 25 opens again first feed inlet 21 with second feed inlet 22, through 6-12 parts of water are added to first feed inlet 21, through 6-12 parts of 30% -40% ethanol are added to second feed inlet 22, carry out the secondary according to above-mentioned mode and draw, mixing tank lower part space is used for storing the thyme extract after adding the emulsifier, and the dark plum extract of the second extraction is stored with the mixture of the first extraction, concentrates two kinds respectively and makes liquid, then mixes two kinds of liquid, obtains the finished product.

Example 1:

taking a dark plum medicinal material in parts by weight, adding 8 times of water, decocting and extracting for 2 times, each time for 3 hours, concentrating until the crude drug content is 0.5g/mL, and filtering; pulverizing herba Thymi into 50 mesh, adding 6 times of 30% ethanol, heating to 65 deg.C, stirring and extracting for 2 times, each time for 1.5 hr, adding tween-80, stirring, and concentrating to obtain extract with crude drug content of 0.5 g/mL; mixing mume fructus solution and thymol solution, and mixing. The use method comprises the following steps: the cow breast medicated bath is carried out after the cow milks are placed into the cow medicated bath cup in the example 1, each medicated bath is carried out for 2-5 seconds, and the medicated bath liquid is fully soaked and adhered to the breasts.

Example 2:

taking a dark plum medicinal material in parts by weight, adding 10 times of water, decocting and extracting for 2 times, each time for 3 hours, and concentrating to ensure that the crude drug content is 1 g/mL; taking thyme medicinal material with the weight part, crushing the thyme medicinal material into 100 meshes, adding 10 times of 40% ethanol, heating to 70 ℃, stirring and extracting for 2 times, extracting for 1.5 hours each time, adding hydrogenated castor oil, stirring uniformly, and concentrating to ensure that the crude drug content is 0.7 g/mL; mixing the mume fructus solution and herba Thymi solution, and mixing. The use method comprises the following steps: the cow breast medicated bath is carried out after the cow milks are placed into the cow medicated bath cup in the example 2, each medicated bath is carried out for 2-5 seconds, and the medicated bath liquid is fully soaked and adhered to the breasts.

Example 3:

determination of fractional inhibitory concentration index sample preparation of example 3: taking the dark plum fruit medicinal material in parts by weight, adding 10 times of water, decocting and extracting for 2 times, each time for 3 hours, and concentrating to ensure that the crude drug content is 0.5 g/mL.

Example 4:

determination of fractional inhibitory concentration index sample preparation of example 4: taking thyme medicinal material with the weight portion, crushing the thyme medicinal material into 50 meshes, adding 12 times of 40% ethanol, heating to 65 ℃, stirring and extracting for 2 times, extracting for 1.5 hours each time, adding sodium fatty alcohol polyoxyethylene ether sulfate, stirring uniformly, and concentrating to ensure that the crude drug content is 0.5 g/mL. And observing the effect enhancement effect of the dark plum extract and the thyme alcohol extract through the graded bacteriostasis concentration index, and determining the optimal proportion between the dark plum extract and the thyme alcohol extract.

The graded bacteriostasis concentration index detection method comprises the following steps:

calculation of FIC index: FIC index is MIC (combination a)/MIC (combination a alone) + MIC (combination B)/MIC (combination B alone).

FIC index interpretation criteria: when FIC is less than or equal to 0.5, the two medicines act synergistically; when FIC is more than 0.5 and less than or equal to 1, the two medicines have additive effect; when FIC is more than 1 and less than or equal to 2, the two medicines have unrelated effects; when FIC > 2, the two drugs are antagonistic. The tests are shown in Table 1.

TABLE 1 Graded inhibitory concentration index test design for examples 3 and 4

Note: 1:2 means that 1 part of the extract is diluted with 1 part of sterile physiological saline. 1:4 means that 1 part of extract is diluted by 3 parts of sterile normal saline and so on. "+" indicates no bacteriostatic effect, and "-" indicates complete bacteriostatic.

Table 1 shows that the dilution factor of example 3 is 1:8, the dilution factor of example 4 is 1:128, the FIC is 0.562 > 0.5, and the ratio converted to crude drug of examples 3 and 4 is 16: 1; the dilution factor of example 3 is 1:8, the dilution factor of example 4 is 1:64, the FIC is 0.625 > 0.5, and the ratio converted into the crude drug of examples 3 and 4 is 8: 1; the dilution factor of example 3 is 1:16, the dilution factor of example 4 is 1:64, the FIC is 0.375 < 0.5, and the ratio converted into the crude drug of examples 3 and 4 is 4: 1; the dilution factor of example 3 is 1:16, the dilution factor of example 4 is 1:32, the FIC thereof is 0.500 to 0.5, and the ratio in terms of crude drug amount of examples 3 and 4 is 2: 1; the dilution factor of example 3 is 1:32, the dilution factor of example 4 is 1:32, the FIC is 0.375 < 0.5, and the ratio converted into the crude drug of examples 3 and 4 is 1: 1; the dilution factor of example 3 is 1:32, the dilution factor of example 4 is 1:16, the FIC is 0.625 > 0.5, and the ratio converted into the crude drug of examples 3 and 4 is 1: 2; the dilution factor of example 3 is 1:32, the dilution factor of example 4 is 1:8, the FIC is 1.125 > 0.5, and the ratio converted into the crude drug of examples 3 and 4 is 1: 4; the dilution factor of example 3 was 1:64, the dilution factor of example 4 was 1:8, the FIC was 1.062 > 0.5, and the ratio of raw drug amounts converted to examples 3 and 4 was 1: 8.

From the results, example 3: in example 4, when the weight ratio of the raw medicines is between 4:1 and 1:1, the two medicines have synergistic antibacterial effect (FIC is less than or equal to 0.5). The combined application of the dark plum extract and the thyme extract within the proportion range has obvious antibacterial effect improvement.

The effect of rapid sterilization in example 2 was observed.

The sterilization test method comprises the following steps: example 2 was diluted with sterile physiological saline at 1:1,1: 2,1: 5, taking 5mL of each concentration, placing the 5mL of each concentration in a corresponding standard test tube, adding 0.1mL of test bacterium liquid, starting timing after uniform oscillation, centrifuging the mixed solution after 5min, removing the supernatant, adding 5mL of sterile physiological saline, uniformly mixing, stopping the bacterium and medicine effect, stopping the sterilization effect of the liquid medicine, sucking 0.1mL of bacterium and medicine mixed solution, coating the plate, and repeating twice. Sterile normal saline is used to replace the liquid medicine, the same operation is carried out, and blank control (only bacteria are added and no medicine is added) is carried out. The sterilization effect is judged by observing the number of the bacterial colonies on the flat plate. The results of the sterilization test of example 2 are shown in the following table

TABLE 2 quick sterilization test results of example 2

The results in Table 2 show that the 5-minute sterilization rate of the product can reach hundreds of percent after the product is diluted by 1, 2 and 5 times in the examples.

The efficacy of example 1 for preventing mastitis in cows was observed.

According to the records of a cattle farm, selecting cattle with similar milk yield, gestational age and lactation period to enter a test cattle group, and determining the cattle to be selected as test cattle according to the clinical symptoms, CMT screening and somatic cell analyzer measurement of the test cattle. The clinical symptoms of the tested cattle are good in mental state, normal in diet, free of swelling, redness, fever and lumps in the breast area, free of pain feeling by touch, free of reduction in milk yield and free of character change visible to naked eyes in milk quality.

And (3) CMT detection: the CMT test result of the tested cattle is negative or suspicious.

And (3) somatic cell detection: the somatic cell count in the milk to be tested must not be more than 50 ten thousand per ml.

Example 1 a bath of cow udder soak was performed using the stock solution; the povidone-iodine solution of the control group is used temporarily according to the conventional use method of a cattle farm.

Animal grouping and administration: 300 healthy lactating cows meeting the inclusion standard are randomly divided into 2 groups according to a cow farm and a cow good, the groups are respectively a medicated bath group of the example 1 and a medicated bath group of the povidone iodine solution, and nipples of the groups are respectively medicated bath with the solution of the example 1 and the solution of the povidone iodine after milking of each test group and are continuously used for 8 weeks.

TABLE 3 Total detection Rate for recessive mastitis in cows

The result shows that the compound preparation in example 1 has a good effect of preventing mastitis of the dairy cattle, is equivalent to a povidone iodine control group, can well control recessive mastitis of a cattle herd, and can replace the povidone iodine.

The above description is only an embodiment of the present invention, and not intended to limit the scope of the present invention, and all modifications of equivalent structures and equivalent processes, which are made by using the contents of the present specification and the accompanying drawings, or directly or indirectly applied to other related technical fields, are included in the scope of the present invention.

Claims

1. The production method of the bactericidal pharmaceutical composition is characterized by comprising the following steps:

s1: taking 1-6 parts of dark plum medicinal materials according to the weight part, putting the dark plum medicinal materials into the lower part of an extraction tank (100), adding 6-60 parts of water, decocting and extracting the raw materials twice for 3 hours each time, and concentrating to obtain the crude drug content of 0.05-2 g/mL;

s2: taking 1-1.5 parts by weight of thyme crushed into 50-100 meshes and 6-12 parts by weight of 30-40% ethanol, putting the thyme and the ethanol into the upper part of an extraction tank (100), heating the extraction tank by using water vapor generated by decoction in S1, controlling the temperature by controlling a valve to control the temperature of the upper part of the extraction tank (100) to be 50-75 ℃, stirring the solution in the extraction process, and extracting for 2 times, wherein each time lasts for 1.5 hours;

the extraction tank (100) described above includes:

a tank body (1);

the channel structure (2), the channel structure (2) is arranged on the side wall of the tank body (1);

the utility model discloses a novel water tank, including the jar body (1), the lateral wall of connection structure (3), connection structure (3) are located the lateral wall of the jar body (1), connection structure (3) include communicating pipe (31), first switch (32), steam pipe (33), go up discharging pipe (34), discharging pipe (35) and filter screen (36) down, the top of communicating pipe (31) is located the lateral wall of the jar body (1), first switch (32) rotate connect in the inside of communicating pipe (31), steam pipe (33) are fixed in the top of communicating pipe (31), go up discharging pipe (34) install in the inside of communicating pipe (31), just go up the top of discharging pipe (34) with the inside upper portion intercommunication of the jar body (1), discharging pipe (35) one end down with the inside lower part intercommunication of the jar body (1), the other end with go up discharging pipe (34) intercommunication, the two filter screens (36) are respectively arranged inside the top end of the upper discharge pipe (34) and inside the lower discharge pipe (35);

the mixing structure (4) is communicated with the bottom end of the communicating pipe (31), the mixing structure (4) comprises a mixing barrel (41), the interior of the mixing barrel (41) is communicated with the bottom end of the communicating pipe (31), and the bottom end of the upper discharging pipe (34) is communicated with the interior of the mixing barrel (41);

the second partition plate (5), the said second partition plate (5) is fixed to the inboard wall of the said tank body (1);

the ventilation structure (6) is arranged inside the second partition plate (5), the ventilation structure (6) comprises an air outlet pipe (61), a ball valve (62) and a warming cavity (63), the air outlet pipe (61) is arranged inside the second partition plate (5), one end of the air outlet pipe (61) is positioned inside the top end of the communicating pipe (31), the ball valve (62) is rotatably connected inside the air outlet pipe (61), the warming cavity (63) is arranged on the inner wall of the upper part of the tank body (1), and the warming cavity (63) is communicated with the air outlet pipe (61) and the vent hole (23);

the stirring structure (7) is rotatably connected to the inside of the tank body (1), the stirring structure (7) comprises a first rotating shaft (71), a first rotating blade (72), a first stirring blade (73) and a second rotating blade (74), two ends of the first rotating shaft (71) are rotatably connected to the top end and the bottom end of the inside of the tank body (1), the first rotating blade (72) is fixed to the middle of the first rotating shaft (71), the first rotating blade (72) is rotated inside the air outlet pipe (61), the two first stirring blades (73) are respectively fixed to the upper portion and the lower portion of the first rotating shaft (71), the second rotating blade (74) is fixed to the top end of the first rotating shaft (71), and the second rotating blade (74) is rotated inside the temperature rising cavity (63);

the linkage structure (8) is rotatably connected to the inside of the communicating pipe (31), the linkage structure (8) comprises a second rotating shaft (81), a second stirring blade (82) and a third rotating blade (83), the second rotating shaft (81) is rotatably connected to the communicating pipe (31) and the inside of the mixing barrel (41), the second stirring blade (82) is fixed to the bottom end of the second rotating shaft (81), the upper part of the inside of the second stirring blade (82) is fixed to the upper part of the second rotating shaft (81), and the third rotating blade (83) is rotatably connected to the inside of the air outlet pipe (61);

and the check valve (9), wherein the check valve (9) is arranged inside the second partition plate (5) and inside the warming cavity (63).

2. The method for producing a bactericidal pharmaceutical composition according to claim 1, the channel structure (2) comprises a first feeding hole (21), a second feeding hole (22), an air vent (23), a first pressure relief valve (24) and a second pressure relief valve (25), the first feed inlet (21) and the second feed inlet (22) are arranged on the side wall of the tank body (1), and the first feed inlet (21) and the second feed inlet (22) are communicated with the lower part and the upper part inside the tank body (1), the vent (23) is arranged on the top surface of the tank body (1), the first pressure release valve (24) and the second pressure release valve (25) are arranged on the side wall of the tank body (1), and the first pressure release valve (24) is communicated with the warming cavity (63), and the second pressure release valve (25) is communicated with the lower part inside the tank body (1).

3. The method for producing a sterilizing pharmaceutical composition according to claim 1, wherein the mixing structure (4) further comprises a discharge port (42), a second switch (43), an addition port (44), a first partition plate (45) and a communication port (46), the discharge port (42) is provided at the bottom end of the side wall of the mixing tub (41), the discharge port (42) is communicated with the lower portion of the interior of the mixing tub (41), the second switch (43) is rotatably connected to the interior of the mixing tub (41), the addition port (44) is provided at the upper end of the side wall of the mixing tub (41), the addition port (44) is communicated with the upper portion of the interior of the mixing tub (41), the first partition plate (45) is fixed to the inner wall of the mixing tub (41), and the communication port (46) is communicated with the upper and lower portions of the interior of the mixing tub (41), and the second switch (43) abuts against the communication port (46).

4. The method for producing a bactericidal pharmaceutical composition according to claim 1, wherein the linkage structure (8) further comprises a first helical gear (84), a second helical gear (85), a rotating rod (86) and a cleaning brush (87), the first helical gear (84) is fixed to the top end of the second rotating shaft (81), the second helical gear (85) is engaged with the first helical gear (84), one end of the rotating rod (86) is fixed to the side wall of the second helical gear (85), the rotating rod (86) is rotatably connected to the inside of the filter screen (36), the cleaning brush (87) is fixed to the other end of the rotating rod (86), and the cleaning brush (87) abuts against the side wall of the filter screen (36).

5. The method for producing a sterilized pharmaceutical composition according to claim 1, wherein a heating plate (9a) is installed on the inner bottom surface of the can body (1).