CN107581354A - A kind of feed addictive of antibacterial and raising biological immune power and preparation method thereof - Google Patents
A kind of feed addictive of antibacterial and raising biological immune power and preparation method thereof Download PDFInfo
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- CN107581354A CN107581354A CN201710704174.XA CN201710704174A CN107581354A CN 107581354 A CN107581354 A CN 107581354A CN 201710704174 A CN201710704174 A CN 201710704174A CN 107581354 A CN107581354 A CN 107581354A
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Abstract
The present invention relates to a kind of antibacterial and the feed addictive for improving biological immune power and preparation method thereof, it is made up of the raw material of following parts by weight：1~15 part of folium eucalypti extract, 5~20 parts of Cortex cinnamomi japonici (Ramulus Cinnamomi) extract, 5~20 parts of sodium citrate, 10~20 parts of royal jelly freeze-dried powder, 5~25 parts of SDPP；Its preparation method comprises the following steps：Folium eucalypti and cassia bark are dried in the shade, crushed, absolute ethyl alcohol is added, ultrasonic extraction after mixing, filtering, filtrate decompression is concentrated, is dried in vacuo, obtain the alcohol extracting thing of folium eucalypti and cassia bark；Folium eucalypti extract, Cortex cinnamomi japonici (Ramulus Cinnamomi) extract, sodium citrate, royal jelly freeze-dried powder and SDPP are mixed again, granulation, and particle is toasted into drying, both.Main composition of the present invention uses all-natural product, safe without toxic side effect, immune response reaction can effectively be strengthened, improve survival rate, health status and the growth performance of animal, alternative conventional antibiotic, and preparation technology is simple, production cost is relatively low, has important society and economic value.
Description
Technical field
The invention belongs to feed additive field, and in particular to a kind of antibacterial and the feed addictive for improving biological immune power
And preparation method thereof.
Background technology
With the high speed development of aquaculture industry of China, modern large-scale cultivation turns into main trend, wherein science and technology cultivation also has
Relatively broad application space.But simple pursuit culture benefit, causes the potential risk formed for a long time in aquaculture
Do not obtain adequate solution.Such as various antibiotic are abused for a long time, medicament residue and drug resistance in animal body is caused to be caused a disease
Bacterium amount reproduction, animal wastes and sewage discharged in cultivation, is not also handled effectively, causes two serious secondary rings
Border is polluted, and then influences the life and existence of the mankind；Secondly, long-term use of antibiotic, animal cell immunity, humoral immunity are caused
Function reduces, so as to influence its production performance.Therefore, use of many advanced countries of the world to antibiotic proposes limitation, such as Europe
Alliance completely forbids in January, 2006 and uses antibiotic as feed addictive.The new feed addictive management rules in China
The use of (No. 168 bulletins) also to Antibiotic Additive is made that regulation and limitation.
However, prohibitting the use of due to antibiotic growth promoter, livestock and poultry infect asking for ethnic specific pathogenetic microorganism
Inscribe getting worse, breeding performonce fo animals decline, production cost increase.Therefore, research and development have no toxic side effect, nothing remains in vivo,
It is pollution-free, have no drug resistance, low cost, the feed addictive of positive effect, have become scientific research and cultivate one of field it is important
Subject under discussion.
The content of the invention
It is an object of the present invention in view of the shortcomings of the prior art, there is provided one kind has antibacterial and improves biological immune power
Feed addictive of effect and preparation method thereof, the feed addictive main composition use all-natural product, safe nothing
Toxic side effect, alternative conventional antibiotic, there is important society and economic value.
The present invention is achieved through the following technical solutions：
A kind of antibacterial and the feed addictive for improving biological immune power, include the raw material of following weight parts：Folium eucalypti extracts
1~15 part of thing, 5~20 parts of Cortex cinnamomi japonici (Ramulus Cinnamomi) extract, 5~20 parts of sodium citrate, 10~20 parts of royal jelly freeze-dried powder, SDPP 5
~25 parts.
Preferably, the feed addictive of a kind of antibacterial and raising biological immune power, the raw material of following weight parts are included：Eucalyptus
5~10 parts of leaf extract, 8~15 parts of Cortex cinnamomi japonici (Ramulus Cinnamomi) extract, 8~15 parts of sodium citrate, 13~17 parts of royal jelly freeze-dried powder, blood plasma egg
10~20 parts of white powder.
Preferably, the feed addictive of a kind of antibacterial and raising biological immune power, the raw material of following weight parts are included：Eucalyptus
7 parts of leaf extract, 11 parts of Cortex cinnamomi japonici (Ramulus Cinnamomi) extract, 11 parts of sodium citrate, 15 parts of royal jelly freeze-dried powder, 15 parts of SDPP.
Folium eucalypti extract：Eucalyptus is the general designation of Myrtaceae Myrtaceae eucalyptus category Eucalyptus plants, originating from Australia
Continent.Eucalyptus leaves contains mass efficient composition, and these materials have strong physiologically active, food, chemical industry, medicine, environment,
Energy etc. has extensive purposes, such as the active material 1 in folium eucalypti extract, 8- cineoles, spathulenol, trans rosin
Celery alcohol, australene, p-cymene, globulol, cryptone, β-phellandrene, melalinol, borneol, limonene and different spoon leaf
Cineol etc., there is stronger antibacterial activity, can effectively suppress and kill most of gram-positive bacterium and partly remove from office blue
Family name's negative bacteria.
Cortex cinnamomi japonici (Ramulus Cinnamomi) extract：Cassia bark is that the bark of camphor tree section plant Chinese cassia tree and stem's epidermis form through drying, also known as Chinese cassia tree, is had
Strong Chinese cassia tree fragrance, mainly originates in the ground such as Guangxi, Guangdong, Yunnan, in addition the also country such as India, Vietnam.Cassia bark alcohol extracting
Thing key component has 9,12- octadecanes dienoic acid methyl ester, 9- octadecenic acids methyl esters, methyl palmitate, anisylacetone etc., studies table
It is bright, its alcohol extracting thing have to Escherichia coli, Pseudomonas fluorescens, Listeria monocytogenes, Lactobacillus saki it is significant suppress and
Deactivation.
Sodium citrate：Sodium citrate is a kind of excellent chelating agent, has a variety of excellent performances, is widely used, is such as used as
Flavor enhancement, buffer, emulsifying agent, swelling agent, stabilizer and preservative etc..Due to prepare the raw material basic source of sodium citrate in
Grain, thus safety non-toxic.Sodium citrate and Cortex cinnamomi japonici (Ramulus Cinnamomi) extract have good synergy, can significantly increase cassia bark and carry
Take the bactericidal activity of thing, the present invention compounds both, products obtained therefrom not only to gram-positive bacterium (staphylococcus aureus,
Hay bacillus, Listeria monocytogenes etc.) there is obvious antibacterial and bactericidal action, to gramnegative bacterium (intestines
Enterohemorrhagic E.coli, Bacterium enteritidis, salmonella typhimurium) also there is significant bactericidal effect.
Royal jelly：Royal jelly is also known as royal jelly, bee milk, emperor's slurry, royal jelly, is the feeding of worker bee hypopharyngeal gland and maxilla glandular secretion
Queen bee and a kind of white or faint yellow sticky slurry of bee larva, mainly contain protein, amino acid, organic acid and dimension
The compositions such as raw element, also a small amount of phytosterol, acetylcholine, AMP, it is the day for collecting nutrition, dietotherapy and health care many integration
Right material, there is antibacterial, anti-oxidant, anti-aging, hypoglycemic, reducing blood lipid, antitumor, adjusting immune and cosmetology etc., other are more
Aspect physiological action.The bacteriostasis of royal jelly is extremely obvious.10-HAD is to Escherichia coli, bacillus subtilis and golden yellow Portugal
Grape coccus shows inhibitory activity.The molten thing of ether and the hydrotrope of royal jelly are to Escherichia coli, pseudomonad, 3 kinds of salmonella
Gram-negative bacteria shows inhibitory action.But royal jelly is only in high concentration to staphylococcus aureus, Candida albicans
There is bacteriostasis, and its suppression on the growth of Escherichia coli, salmonella, Staphylococcus aureus and hay bacillus is influenceed by pH,
Bacteriostatic activity under neutral environment is markedly less than alkaline environment.
SDPP：SDPP is that collection through the qualified animal new blood of quarantining is raw material, through anti-freezing, cold
But, the high-quality protein of the technological process such as filtering, separation, nanofiltration, refrigeration, high-pressure homogeneous, spray drying, dry powder quickly cooling production
Feedstuff, there is the advantages of palatability is good, protein content is high, amino acid balance and utilization rate are high, especially process
In remain the activity of various functions immunoglobulin in former blood plasma simultaneously, immunoglobulin is by acting on the mucous membrane of body
Organize (such as small intestine, breathing, reproduction) to play the function of the reaction of enhancing immune response and lifting vaccine valence, improve body pair
The resistance of the antigens such as pathogen, virus and protozoon, to reduce the morbidity of pathogenic bacteria, virus and protozoal infection condition lower body
Rate and the death rate, improve the survival rate, health status and growth performance of animal.
A kind of preparation method of the feed addictive of antibacterial and raising biological immune power, comprises the following steps：
(1) take folium eucalypti lucifuge to dry in the shade, be crushed to 80 mesh, by the mass body of folium eucalypti quality (kg) and Extraction solvent volume (L)
Product is than being 1：9 add absolute ethyl alcohol, are placed in and are cleaned by ultrasonic in instrument after mixing, ultrasonic extraction, each ultrasonic 20min, totally 4 times, mistake
Filter, filtrate decompression is concentrated, and is dried in vacuo, and both obtains folium eucalypti extract；
(2) take cassia bark lucifuge to dry in the shade, be crushed to 80 mesh, by the mass body of cassia bark quality (kg) and Extraction solvent volume (L)
Product is than being 1：9 add absolute ethyl alcohol, are placed in and are cleaned by ultrasonic in instrument after mixing, ultrasonic extraction, each ultrasonic 20min, totally 4 times, mistake
Filter, filtrate decompression is concentrated, and is dried in vacuo, and both obtains Cortex cinnamomi japonici (Ramulus Cinnamomi) extract；
(3) weigh by weight folium eucalypti extract obtained by step (1), Cortex cinnamomi japonici (Ramulus Cinnamomi) extract obtained by step (2), sodium citrate,
Royal jelly freeze-dried powder and SDPP, after being well mixed, it is manufactured into particle diameter and is 0.5~1cm particle, and particle is placed in 80
15~30min is toasted in DEG C baking oven, is dried, both feed addictive of the present invention.
Beneficial effects of the present invention：
1st, the present invention compounds folium eucalypti extract and Cortex cinnamomi japonici (Ramulus Cinnamomi) extract and sodium citrate, three's synergy, can not only
Effectively suppress and kill gram-positive bacteria, also there is obvious inhibitory action to Gram-negative bacteria, significantly enhance folium eucalypti
The bactericidal activity and antimicrobial spectrum of extract and Cortex cinnamomi japonici (Ramulus Cinnamomi) extract.
2nd, inventive compound royal jelly and SDPP rich in nutrition content, and with antibacterial, anti-oxidant, regulation
Many-sided physiological action such as immune, applied to immune response reaction in feed addictive, can be strengthened, improve body to disease
The resistance of the antigens such as opportunistic pathogen, virus and protozoon, reduce pathogenic bacteria, virus and protozoal infection condition lower body the incidence of disease and
The death rate, improve the survival rate, health status and growth performance of animal.
3rd, main composition of the present invention uses all-natural product, does not produce drug resistance, safe without toxic side effect, can replace
For conventional antibiotic, and preparation technology is simple, and production cost is relatively low, has important society and economic value.
Embodiment
In order to make the purpose , technical scheme and advantage of the present invention be clearer, with reference to embodiments, to the present invention
It is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to
Limit the present invention.
Embodiment 1~5
A kind of antibacterial of the present invention is listed in table 1 and improves the feed addictive of biological immune power 5 are specific
Embodiment.
Table 1
A kind of antibacterial described in embodiment 1~5 is prepared in accordance with the following steps and improves the feed addictive of biological immune power：
(1) take folium eucalypti lucifuge to dry in the shade, be crushed to 80 mesh, by the mass body of folium eucalypti quality (kg) and Extraction solvent volume (L)
Product is than being 1：9 add absolute ethyl alcohol, are placed in and are cleaned by ultrasonic in instrument after mixing, ultrasonic extraction, each ultrasonic 20min, totally 4 times, mistake
Filter, filtrate decompression is concentrated, and is dried in vacuo, and both obtains folium eucalypti extract；
(2) take cassia bark lucifuge to dry in the shade, be crushed to 80 mesh, by the mass body of cassia bark quality (kg) and Extraction solvent volume (L)
Product is than being 1：9 add absolute ethyl alcohol, are placed in and are cleaned by ultrasonic in instrument after mixing, ultrasonic extraction, each ultrasonic 20min, totally 4 times, mistake
Filter, filtrate decompression is concentrated, and is dried in vacuo, and both obtains Cortex cinnamomi japonici (Ramulus Cinnamomi) extract；
(3) weigh by weight folium eucalypti extract obtained by step (1), Cortex cinnamomi japonici (Ramulus Cinnamomi) extract obtained by step (2), sodium citrate,
Royal jelly freeze-dried powder and SDPP, after being well mixed, it is manufactured into particle diameter and is 0.5~1cm particle, and particle is placed in 80
15~30min is toasted in DEG C baking oven, is dried, both feed addictive of the present invention.
Application Example 1
A kind of antibacterial of the present invention and inhibitory action of the feed addictive to pathogen for improving biological immune power
1st, test sample：A kind of antibacterial and the feed addictive of raising biological immune power prepared by above-described embodiment 1~5.
2nd, pathogen is tested：Staphylococcus aureus, Escherichia coli, pseudomonas aeruginosa, Candida albicans.
3rd, test culture medium：Bacterium is beef extract-peptone bacteria culture media；Candida albicans is husky Bao Shi culture mediums.
4th, method of testing：Inhibition zone size is determined using lysoplate assay.Key step is as follows：
(1) feed addictive prepared by above-described embodiment 1~5 is configured to by the molten of 40mg/mL with 75% ethanol respectively
Liquid；
(2) after cause of disease bacteria strain to be measured being activated and diluted respectively, 0.1mL bacteria suspensions even spread is taken to culture medium flat plate
On, after cultivating 1h in 37 DEG C of incubators, often containing only the hole of making a call to 3 diameter 6mm is spaced a distance on bacterium flat board, in three
Horn shape is arranged, and the feed addictive solution of 100 μ L steps (1) preparation is added into every hole, blank control is done with 75% ethanol,
Each processing is repeated 3 times, results averaged；
(3) culture dish is upside down in incubator, 37 DEG C of incubated 48h (Candida albicans culture 72h), takes out measure suppression
The diameter of bacterium circle, the fungistatic effect of feed addictive prepared by comparing embodiment 1~5.
5th, test result：Testing result is as shown in table 2 below.
A kind of 2 antibacterial of table and the feed addictive Antibacterial Activity result for improving biological immune power
Compared with the control, embodiment 1~5 can significantly inhibit staphylococcus aureus, Escherichia coli, P. aeruginosa
Bacterium, the growth of Candida albicans, wherein, the action effect of embodiment 3 is most notable, compared with the control, staphylococcus aureus, large intestine
Bacillus, pseudomonas aeruginosa, the inhibition zone of Candida albicans increase 19.9mm, 15.3mm, 15.3mm, 17.1mm respectively, for this
The most preferred embodiment of invention.
Application Example 2
The influence of a kind of antibacterial of the present invention and the feed addictive of raising biological immune power to Growth Performance of Weaning Piglets
1st, experimental design
This experiment is designed using single factor experiment, and experiment chooses that body weight is close, male and female ratio is consistent, is in a good state of health
DLY 15kg or so weanling pig 126.Test pig is randomly divided into 6 groups, and each 3 repetitions for the treatment of group are each to repeat 7
Pig.Control group fed basal diet, prepared by above-described embodiment 1~5 that test group feeds basal diet addition 10g/kg respectively
Feed addictive.
2nd, feeding management
Test and experiment pig house, feeding equipment thoroughly clean and sterilize before Jian begins.Test pig was raised in advance using 5 days,
Between pre-feeding period, pig is micro-adjusted, before making on-test, difference is little between each group test pig.Formal test starts
On the same day, test pig is carried out to weigh on an empty stomach.During experiment, the feeding management of test pig and immune according to pig farm conventinal breeding
Rule of management operation is carried out.Test period is 30 days, during experiment, will record the feed consumption situation of each group test pig daily.
3rd, test index assay method
On-test and terminate the same day morning, to test pig carry out on an empty stomach weigh, calculate respectively test pig starting weight and
End weight.During experiment, observation and the feed intake and mental status of record test pig are paid attention to.Finally according to experimental period record case meter
Calculate：Test pig average daily gain, average daily gain, feed-weight ratio, diarrhea rate and survival rate.
Average daily gain=(test pig end weight-test pig starting weight)/total number of days of experiment
Average daily gain=(testing material scale of feeding-feed surplus)/experiment number of days
Feed-weight ratio=average daily gain/average daily gain
Diarrhea rate=summation × 100% of daily grice diarrhoea head number during formal/(tests piglet sum × total day of experiment
Number)
4th, result of the test：Testing result is as shown in table 3 below.
Influence of the feed addictive of a kind of 3 antibacterial of table and raising biological immune power to Growth Performance of Weaning Piglets
| Test index | Embodiment 1 | Embodiment 2 | Embodiment 3 | Embodiment 4 | Embodiment 5 | Control |
| Average daily gain (g/d) | 563 | 570 | 579 | 572 | 568 | 506 |
| Average daily gain (g/d) | 810 | 813 | 788 | 792 | 790 | 964 |
| Feed-weight ratio (g/g) | 1.44 | 1.42 | 1.36 | 1.38 | 1.39 | 1.91 |
| Diarrhea rate (%) | 0.53 | 0.47 | 0.25 | 0.31 | 0.39 | 3.56 |
| Survival rate (%) | 100 | 100 | 100 | 100 | 100 | 85.7 |
Compared with the control, the feed addictive that prepared by embodiment 1~5 can significantly improve the average daily gain of weanling pig
And survival rate, average daily gain, feed-weight ratio and diarrhea rate are reduced, wherein, the action effect of embodiment 3 is most notable, and to photograph
Than weanling pig average daily gain improves 14.43%, and survival rate improves 16.69%, and average daily gain reduces by 18.26%, material
Again than reducing by 28.80%, diarrhea rate reduces by 92.98%, and it is highly preferred embodiment of the present invention to show embodiment 3.
5th, conclusion
A kind of antibacterial of the present invention and the feed addictive of raising biological immune power can significantly improve the flat of weanling pig
Equal daily gain and survival rate, reduce average daily gain, feed-weight ratio and diarrhea rate, alternative conventional antibiotic, prolonged application sheet
The feed addictive of invention, be advantageous to improve efficiency of feed utilization, reduce aquaculture cost, increase economic efficiency, have a extensive future.
Claims (4)
1. a kind of antibacterial and the feed addictive for improving biological immune power, it is characterised in that the raw material including following weight parts：
1~15 part of folium eucalypti extract, 5~20 parts of Cortex cinnamomi japonici (Ramulus Cinnamomi) extract, 5~20 parts of sodium citrate, 10~20 parts of royal jelly freeze-dried powder, blood plasma
5~25 parts of albumen powder.
2. a kind of antibacterial according to claim 1 and the feed addictive for improving biological immune power, it is characterised in that including
The raw material of following weight parts：5~10 parts of folium eucalypti extract, 8~15 parts of Cortex cinnamomi japonici (Ramulus Cinnamomi) extract, 8~15 parts of sodium citrate, royal jelly
13~17 parts of freeze-dried powder, 10~20 parts of SDPP.
3. a kind of antibacterial according to claim 2 and the feed addictive for improving biological immune power, it is characterised in that including
The raw material of following weight parts：7 parts of folium eucalypti extract, 11 parts of Cortex cinnamomi japonici (Ramulus Cinnamomi) extract, 11 parts of sodium citrate, royal jelly freeze-dried powder 15
Part, 15 parts of SDPP.
A kind of 4. system of the feed addictive of a kind of antibacterial as described in any one of claims 1 to 3 and raising biological immune power
Preparation Method, comprise the following steps：
(1) take folium eucalypti lucifuge to dry in the shade, be crushed to 80 mesh, by the mass volume ratio of folium eucalypti quality (kg) and Extraction solvent volume (L)
For 1：9 add absolute ethyl alcohol, are placed in and are cleaned by ultrasonic in instrument after mixing, ultrasonic extraction, each ultrasonic 20min, totally 4 times, filter, will
Filtrate decompression concentrates, and vacuum drying, both obtains folium eucalypti extract；
(2) take cassia bark lucifuge to dry in the shade, be crushed to 80 mesh, by the mass volume ratio of cassia bark quality (kg) and Extraction solvent volume (L)
For 1：9 add absolute ethyl alcohol, are placed in and are cleaned by ultrasonic in instrument after mixing, ultrasonic extraction, each ultrasonic 20min, totally 4 times, filter, will
Filtrate decompression concentrates, and vacuum drying, both obtains Cortex cinnamomi japonici (Ramulus Cinnamomi) extract；
(3) folium eucalypti extract obtained by step (1), Cortex cinnamomi japonici (Ramulus Cinnamomi) extract, sodium citrate, queen bee obtained by step (2) are weighed by weight
Freeze-dried powder and SDPP are starched, after being well mixed, particle diameter is manufactured into and is 0.5~1cm particle, and particle is placed in 80 DEG C of bakings
In case toast 15~30min, dry, both feed addictive of the present invention.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114424797A (en) * | 2020-10-29 | 2022-05-03 | 洪江市金土地生态农业有限责任公司 | Feed additive for improving pig immunity, preventing swine fever and improving lean meat percentage |
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|---|---|---|---|---|
| CN102934644A (en) * | 2012-11-13 | 2013-02-20 | 江苏大学 | Mixture of natural plant extracts and preparation method and application of mixture |
| CN104489332A (en) * | 2014-12-15 | 2015-04-08 | 平顶山市九州同心饲料有限公司 | No-antibiotic feed for piglet and preparation process thereof |
| CN105767532A (en) * | 2016-03-22 | 2016-07-20 | 江西天佳动物药业有限公司 | Compound plant essential oil-type feed additive capable of replacing antibiotics |
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2017
- 2017-08-16 CN CN201710704174.XA patent/CN107581354A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102934644A (en) * | 2012-11-13 | 2013-02-20 | 江苏大学 | Mixture of natural plant extracts and preparation method and application of mixture |
| CN104489332A (en) * | 2014-12-15 | 2015-04-08 | 平顶山市九州同心饲料有限公司 | No-antibiotic feed for piglet and preparation process thereof |
| CN105767532A (en) * | 2016-03-22 | 2016-07-20 | 江西天佳动物药业有限公司 | Compound plant essential oil-type feed additive capable of replacing antibiotics |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114424797A (en) * | 2020-10-29 | 2022-05-03 | 洪江市金土地生态农业有限责任公司 | Feed additive for improving pig immunity, preventing swine fever and improving lean meat percentage |
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