CN111616988B - Anti-aging skin care product and preparation method thereof - Google Patents

Anti-aging skin care product and preparation method thereof Download PDF

Info

Publication number
CN111616988B
CN111616988B CN202010582275.6A CN202010582275A CN111616988B CN 111616988 B CN111616988 B CN 111616988B CN 202010582275 A CN202010582275 A CN 202010582275A CN 111616988 B CN111616988 B CN 111616988B
Authority
CN
China
Prior art keywords
oil
extract
resin
parts
seed oil
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN202010582275.6A
Other languages
Chinese (zh)
Other versions
CN111616988A (en
Inventor
不公告发明人
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Guangzhou Xiyan Biotechnology Co.,Ltd.
Original Assignee
Guangxi Mandili Biotechnology Co ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Guangxi Mandili Biotechnology Co ltd filed Critical Guangxi Mandili Biotechnology Co ltd
Priority to CN202010582275.6A priority Critical patent/CN111616988B/en
Publication of CN111616988A publication Critical patent/CN111616988A/en
Application granted granted Critical
Publication of CN111616988B publication Critical patent/CN111616988B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
    • A61K8/922—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof of vegetable origin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/19—Cosmetics or similar toiletry preparations characterised by the composition containing inorganic ingredients
    • A61K8/23—Sulfur; Selenium; Tellurium; Compounds thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9789—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/08—Anti-ageing preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
    • A61K2800/59—Mixtures
    • A61K2800/592—Mixtures of compounds complementing their respective functions
    • A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
    • A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95

Abstract

The invention discloses an anti-aging skin care product and a preparation method thereof, wherein the skin care product is compound essential oil, and the compound essential oil comprises the following raw materials: the sunflower seed oil, the evening primrose oil, the rose hip oil, the sea buckthorn fruit oil and the Augusman resin extract have good anti-aging and anti-wrinkle skin care effects.

Description

Anti-aging skin care product and preparation method thereof
Technical Field
The invention relates to the field of cosmetics, in particular to an anti-aging skin care product and a preparation method thereof.
Background
The essential oil is divided into diluted compound essential oil and undiluted single essential oil, the volatility of the essential oil is very strong, and the essential oil can be volatilized quickly once contacting air. The essential oil is extracted from plants containing the balsamic glands, the balsamic glands of different plants are distributed differently, some are on petals and leaves, and some are on rhizomes and trunks; essential oils are composed of chemical molecules such as terpenes, aldehydes, esters, and alcohols, and are called "oils" because of their high fluidity, but they are fundamentally different from vegetable oils that are routinely seen.
The single essential oil can not be directly used, has no lubrication degree, high concentration and strong irritation, needs to be diluted by base oil, is easy to burn skin, and is common in base oil such as jojoba oil, sweet almond oil, grape seed oil, rose hip oil and the like.
The compound essential oil is prepared by mixing more than two single-component essential oils and base oil according to a certain proportion, and can be directly used for skin care. Each compound essential oil has the targeted effects of whitening, moisturizing, resisting wrinkles and the like; the compound essential oil is prepared, can be used immediately, is prepared into a finished product after combination and blending, and is convenient to use. In order to meet the requirement of people on aging prevention, the research on the compound essential oil with the effects of resisting aging and wrinkles is a constantly-explored target.
Disclosure of Invention
In view of the defects of the prior art, the invention adopts the following specific technical scheme:
the anti-aging skin care product is compound essential oil, and the compound essential oil comprises the following raw materials: sunflower (Helianthus ANNUUS) seed oil, evening primrose (Oenothera BIENNIS) oil, Rosa rugiginosa (Rosa rugiginosa) seed oil, and Hippophae RHAMNOIDES (Hippophae RHAMNOIDES) fruit oil.
Preferably, the anti-aging skin care product is compound essential oil which is prepared from the following raw materials in parts by weight: 30-50 parts of sunflower (Helianthus ANNUUS) seed oil, 30-50 parts of evening primrose (Oenothera BIENNIS) oil, 0.5-5 parts of ROSA rugosa (Rosa RUBIGINOSA) seed oil and 0.5-5 parts of HIPPOPHAE RHAMNOIDES (Hippophae RHAMNOIDES) fruit oil.
Preferably, the anti-aging skin care product is compound essential oil which is prepared from the following raw materials in parts by weight: 30-50 parts of sunflower (Helianthus ANNUUS) seed oil, 30-50 parts of evening primrose (Oenothera BIENNIS) oil, 0.5-5 parts of ROSA rugosa (Rosa RUBIGINOSA) seed oil, 0.5-5 parts of HIPPOPHAE RHAMNOIDES (Hippophae RHAMNOIDES) fruit oil and 0.5-5 parts of Aucumea klaneana resin extract.
Preferably, the anti-aging skin care product is compound essential oil which is prepared from the following raw materials in parts by weight: 30-50 parts of sunflower (Helianthus ANNUUS) seed oil, 30-50 parts of evening primrose (Oenothera BIENNIS) oil, 0.5-5 parts of ROSA rusticana (Rosa RUBIGINOSA) seed oil, 0.5-5 parts of sea buckthorn (Hippophae RHAMNOIDES) fruit oil, 0.5-5 parts of Aucumea klainiana resin extract and 0.5-5 parts of unilaterally flowering (Orthilia SECUNDA) extract.
The inventor finds that if the compound essential oil is only compounded by the rosa multiflora seed oil and the seabuckthorn fruit oil, the anti-aging and anti-wrinkle effects of the compound essential oil are poor due to the low activity of anti-aging and anti-wrinkle components of the rosa multiflora seed oil and the seabuckthorn fruit oil; according to the invention, on the basis of only compounding the rosa roxburghii tratt seed oil and the seabuckthorn fruit oil in the prior art, the rosa roxburghii tratt resin extract and the unilaterally flower extract with specific contents are added to be compounded with the rosa roxburghii tratt seed oil and the seabuckthorn fruit oil with specific contents, and by utilizing high-activity anti-aging and anti-wrinkle components contained in the rosa roxburghii tratt resin extract and the unilaterally flower extract, the technical problem that compound essential oil compounded by only the rosa roxburghii tratt seed oil and the seabuckthorn fruit oil in the prior art has poor anti-aging and anti-wrinkle effects is solved, and the anti-aging.
The invention adds two high-activity extracts of the Oregamann resin extract and the unilaterally flower extract at the same time, synergizes and supplements each other, on one hand, the anti-wrinkle active ingredient contained in the invention can stimulate cell metabolism and promote the regeneration of skin collagen elastic fibers, thereby improving the skin and preventing the generation of new wrinkles; on the other hand, the anti-wrinkle active ingredient is used as an antioxidant to prevent skin subcutaneous fat from being oxidized, enhance the activity of skin epidermis and dermal cells, avoid skin premature senility and further achieve the effects of anti-wrinkle and skin care.
The preparation method of the anti-aging compound essential oil comprises the following steps: mixing semen Helianthi (Helianthus ANNUUS) seed oil, evening primrose (Oenothera BIENNIS) oil, Rosa RUBIGINOSA (Rosa RUBIGINOSA) seed oil and fructus Hippophae (Hippophae RHAMNOIDES) fruit oil uniformly according to formula, heating to 50-70 deg.C, adding Aucoumea KLAINEANA resin extract and Orthosiphon (Ortholinia SECURNDA) extract, stirring at 50-70 deg.C for 10-30min, stirring at a rotation speed of 100 and 200rpm, cooling to 25-35 deg.C, filtering, inspecting, discharging, and packaging.
The raw materials of the anti-aging skin care product are specifically introduced as follows:
sunflower (helioanthus ANNUUS) seed oil, CAS number: 8001-21-6, is rich in linoleic acid, vitamin E, phytosterol, phospholipid, carotene, and other natural moisturizing and antioxidant nutritional ingredients, and is suitable for whole body use. The skin care base oil can rapidly lock water in a sebum layer and supply various natural nutrients and healthy oil required by functions, has strong water retention capacity, enables a sebum membrane to be in a healthy state constantly, can promote self-repair of damaged and sensitive skin, achieves the effects of continuously preserving moisture and locking water, lubricating the skin, resisting wrinkles and preventing looseness, and is common skin care base oil.
Evening primrose (OENOTHERA BIENNIS) oil, CAS number: 65546-85-2, contains rich gamma-linoleic acid, is often used for treating skin allergy, eczema, dryness, itching, etc., and can rebuild skin protective membrane, retain skin moisture, improve acne and mottle caused by endocrine disturbance, delay aging, improve skin quality, and promote hair luster.
Sea buckthorn (HIPPOPHAE RHAMNOIDES) fruit oil, CAS number: 90106-68-6, can inhibit expression of UVB-mediated metalloprotease, and has effects in eliminating superoxide radical, resisting wrinkle, and delaying aging.
Red rose (ROSA RUBIGINOSA) seed oil, EINECS No.: 927-418-4. The skin conditioner has the main effects of resisting oxidation, preventing alopecia and nourishing hair, has no acne-causing property and is safe, and the promotion effect on the generation of ceramide indicates that the ceramide content in sebum can be increased to play a role in softening skin; has effect in inhibiting elastase, resisting wrinkle, and resisting aging.
Macadamia nut (MECADAMIA TERNIFOLIA) seed oil, CAS No.: 128497-20-1, is rich in unsaturated fatty acids, mainly contains oleic acid and palmitic acid, and has effects in preventing heart disease, regulating and controlling blood sugar level, and improving lipid metabolism of diabetic. The cosmetic is mainly used for moisturizing, skin care and sun protection, and the prior art does not use the cosmetic for resisting wrinkles.
The resin extract of Aucumea (Aucoumea KLAINEANA) contains abundant volatile oil, carene as main ingredient, and nonvolatile substances such as phenols, and is mainly used as skin conditioner in daily chemical products, and is not used in anti-wrinkle cosmetics in the prior art.
Extract of unilateral flower (Orthilia SECUNDA) mainly contains arbutin, hydroquinone, coumarin, vitamin C, resin, flavonoids, saponin, tartaric acid and citric acid, and has antitumor, antiinflammatory, blood stasis dispelling, antibacterial, diuretic and analgesic effects. The inventor researches and discovers that the unilateral flower (Orthilia SECUNDA) extract has certain anti-wrinkle effect, which is not reported in cosmetic application.
Preferably, the preparation method of the augustum resin extract comprises the following steps:
s1, pretreatment: adding 1-2mol/L NaOH solution into the Oregamann resin, adjusting the pH value to 8-10, wherein the mass ratio of the Oregamann resin to the 1-2mol/L NaOH solution is 1: (2-20), stirring, mixing, standing for 10-30min, filtering, washing with water, and drying for later use;
s2, supercritical fluid extraction: extracting the Auguman resin treated in the step S1 by adopting a supercritical fluid extraction technology;
extraction conditions are as follows: extraction pressure: 6-12 MPa; the extraction temperature is as follows: 32-40 ℃; CO 22Flow rate: 7-12L/h; extract (Cuiyu)Taking time: 1.5-2.5 h; the entrainer is 1-butyl-3-methylimidazole hexafluorophosphate, the addition amount is 1-5 wt% of the Oregaman resin, and the entrainer flow rate is as follows: 0.2-2 mL/min;
s3, purifying the extract: adding the extract obtained in the step S2 into an ionic liquid, wherein the volume ratio of the extract to the ionic liquid is 10: (30-70), wherein the ionic liquid is 1-propyl-3-methylimidazolium tetrafluoroborate; introducing CO2，CO2Introducing at a speed of 1-2mL/min for 3-10min, oscillating, and stopping introducing CO2Standing for layering, separating liquid, collecting an organic layer, adding a drying agent for drying, wherein the mass ratio of the drying agent to the ionic liquid is 1: (1-5) filtering to obtain the Oregamann resin extract.
The invention is to supercritical CO2The fluid extract is added with entrainer to enhance supercritical CO2And (3) dissolving out the anti-wrinkle active ingredients in the resin by the fluid, so that most of the anti-wrinkle active ingredients in the Oregamann resin are extracted.
Preferably, the drying agent is at least one selected from magnesium sulfate, sodium sulfate and anhydrous copper sulfate. Preferably, the drying agent is a mixture of magnesium sulfate and anhydrous copper sulfate according to a mass ratio of 5: 1.
When anhydrous copper sulfate is used, the anhydrous copper sulfate can be mixed with other drying agents and then dried to serve as an indicator, and if the drying agents do not turn blue, the liquid can be obtained after direct filtration.
The invention has the beneficial effects that: the inventor finds that if the compound essential oil is only compounded by the rosa multiflora thunb seed oil and the seabuckthorn fruit oil, the anti-wrinkle effect of the compound essential oil is poor due to the low activity of anti-wrinkle components in the rosa multiflora thunb seed oil and the seabuckthorn fruit oil; according to the invention, on the basis of only compounding the rosa roxburghii tratt seed oil and the seabuckthorn fruit oil in the prior art, the rosa roxburghii tratt resin extract and the unilaterally flower extract with specific contents are added to be compounded with the rosa roxburghii tratt seed oil and the seabuckthorn fruit oil with specific contents, and by utilizing high-activity anti-wrinkle components contained in the rosa roxburghii tratt resin extract and the unilaterally flower extract, the technical problem of poor anti-wrinkle effect of the compound essential oil compounded by only adopting the rosa roxburghii tratt seed oil and the seabuckthorn fruit oil in the prior art is solved, and the anti-aging.
The invention adds two high-activity extracts of the Oregamann resin extract and the unilaterally flower extract at the same time, synergizes and supplements each other, on one hand, the anti-wrinkle active ingredient contained in the invention can stimulate cell metabolism and promote the regeneration of skin collagen elastic fibers, thereby improving the skin and preventing the generation of new wrinkles; on the other hand, the anti-wrinkle active ingredient is used as an antioxidant to prevent skin subcutaneous fat from being oxidized, enhance the activity of skin epidermis and dermal cells, avoid skin premature senility and further achieve the effects of anti-wrinkle and skin care.
Detailed Description
The specific parameters of some substances in the examples and comparative examples of the invention are as follows:
1-propyl-3-methylimidazolium tetrafluoroborate, the structural formula is as follows:
Figure BDA0002552767110000061
the preparation method is shown in the following documents: ionic liquids of imidazole like Yuanzhi, Cai Yuan, Huangdeying [ PMIm ]]Synthesis and characterization of BF4]，Science&Technology Information，2010,35：68,91。
1-butyl-3-methylimidazolium hexafluorophosphate, the structural formula is as follows:
Figure BDA0002552767110000062
the preparation method is shown in the following documents: ionic liquid [ Bmim ] of Sinkiang Phoebia, Deng Tianlong, Liuming Ming, etc]Br and [ Bmim ]]PF6Synthesis of (1) and study of physicochemical Properties thereof [ J]University of Tianjin science, 2014,29 (5): 42-47.
The method for preparing the augustum resin extract of examples 1-2 and comparative example 3 comprises the following steps:
s1, pretreatment: adding 1.5mol/L NaOH solution into the Oregamann resin, adjusting the pH value to 9, wherein the mass ratio of the Oregamann resin to the 1.5mol/L NaOH solution is 1: 9, stirring, mixing, standing for 20min, filtering, washing with water, and drying for later use;
s2, supercritical fluid extraction: extracting the Auguman resin treated in the step S1 by adopting a supercritical fluid extraction technology to obtain an extract liquid;
extraction conditions are as follows: extraction pressure: 10 MPa; the extraction temperature is as follows: 36 ℃; CO 22Flow rate: 10L/h; extraction time: 2 h; the entrainer is 1-butyl-3-methylimidazole hexafluorophosphate, the addition amount of the entrainer is 3 wt% of the Oregaman resin, and the entrainer flow rate is as follows: 0.3 mL/min;
s3, purifying the extract: adding the extract obtained in the step S2 into an ionic liquid, wherein the volume ratio of the extract to the ionic liquid is 10: 50, the ionic liquid is 1-propyl-3-methylimidazole tetrafluoroborate; introducing CO2，CO2Introducing at a speed of 1.5mL/min for 5min, oscillating, and stopping introducing CO2Standing for layering, separating liquid, collecting an organic layer, adding a drying agent for drying, wherein the mass ratio of the drying agent to the ionic liquid is 1: and 4, mixing the magnesium sulfate and anhydrous copper sulfate according to the mass ratio of 5:1, and filtering to obtain the Augusman resin extract.
In the examples and comparative examples, the same commercially available extract of one lateral flower (Orthilia SECUNDA) or one lateral flower (Orthilia SECUNDA) prepared by the following method may be used: grinding the dried unilaterally flower, sieving with a 100-mesh sieve, adding 87 wt% ethanol water solution, wherein the mass ratio of unilaterally flower powder to 87 wt% ethanol water solution is 3: 37; reflux-extracting at 77 deg.C for 3.5h, centrifuging, filtering, concentrating the filtrate under reduced pressure, and drying.
In the examples and comparative examples, the same commercially available raw materials were used unless otherwise specified.
Comparative example 1
An anti-aging compound essential oil is prepared from the following raw materials in parts by weight: 46 parts of sunflower (Helianthus ANNUUS) seed oil, 50 parts of evening primrose (Oenothera BIENNIS) oil, 2 parts of ROSA rugosa (Rosa rugiginosa) seed oil and 1.5 parts of sea buckthorn (HIPPOPHAE RHAMNOIDES) fruit oil.
The preparation method of the anti-aging compound essential oil comprises the following steps: mixing semen Helianthi (Helianthus ANNUUS) oil, evening primrose (Oenothera BIENNIS) oil, Rosa rugiginosa (Rosa RUBIGINOSA) seed oil and fructus Hippophae (Hippophae RHAMNOIDES) fruit oil uniformly according to formula, heating to 58 deg.C, adding Aucumea klainiana (Aucumea klainiana) resin extract, stirring at 58 deg.C for 25min, stirring at 120rpm, cooling to 30 deg.C, filtering, inspecting, discharging, and packaging.
Example 1
An anti-aging compound essential oil is prepared from the following raw materials in parts by weight: 45 parts of sunflower (Helianthus ANNUUS) seed oil, 50 parts of evening primrose (Oenothera BIENNIS) oil, 2 parts of ROSA rustica (Rosa RUBIGINOSA) seed oil, 1.5 parts of HIPPOPHAE RHAMNOIDES (Hippophae RHAMNOIDES) fruit oil, and 1 part of Aucumea KLAINEANA resin extract.
The preparation method of the anti-aging compound essential oil comprises the following steps: mixing semen Helianthi (Helianthus ANNUUS) oil, evening primrose (Oenothera BIENNIS) oil, Rosa rugiginosa (Rosa RUBIGINOSA) seed oil and fructus Hippophae (Hippophae RHAMNOIDES) fruit oil uniformly according to formula, heating to 58 deg.C, adding Aucumea klainiana (Aucumea klainiana) resin extract, stirring at 58 deg.C for 25min, stirring at 120rpm, cooling to 30 deg.C, filtering, inspecting, discharging, and packaging.
Example 2
An anti-aging compound essential oil is prepared from the following raw materials in parts by weight: 45 parts of sunflower (Helianthus ANNUUS) seed oil, 50 parts of evening primrose (Oenothera BIENNIS) oil, 2 parts of ROSA rusticana (Rosa RUBIGINOSA) seed oil, 1.5 parts of sea buckthorn (Hippophae RHAMNOIDES) fruit oil, 0.5 part of Aucumaria klainiana (Aucumea klainiana) resin extract, and 0.5 part of unilaterally florum (Orthilia SECUNDA) extract.
The preparation method of the anti-aging compound essential oil comprises the following steps: mixing semen Helianthi (Helianthus ANNUUS) oil, evening primrose (Oenothera BIENNIS) oil, Rosa RUBIGINOSA (Rosa RUBIGINOSA) seed oil and fructus Hippophae (Hippophae RHAMNOIDES) fruit oil uniformly according to formula, heating to 58 deg.C, adding Aucumea KLAINEANA (Aucumea klainea) resin extract and unilaterally flower (Orthilia SECUNDA) extract, stirring at 58 deg.C for 25min, stirring at 120rpm, cooling to 30 deg.C, filtering, inspecting, discharging, and packaging.
Comparative example 2
An anti-aging compound essential oil is prepared from the following raw materials in parts by weight: 45 parts of sunflower (Helianthus ANNUUS) seed oil, 50 parts of evening primrose (Oenothera BIENNIS) oil, 2 parts of ROSA rusticana (Rosa RUBIGINOSA) seed oil, 1.5 parts of HIPPOPHAE RHAMNOIDES (Hippophae RHAMNOIDES) fruit oil, and 1 part of unilaterally flowering plant (Orthilia SECUNDA) extract.
The preparation method of the anti-aging compound essential oil comprises the following steps: mixing semen Helianthi (Helianthus ANNUUS) seed oil, evening primrose (Oenothera BIENNIS) oil, Rosa rugiginosa (Rosa rugiginosa) seed oil and fructus Hippophae (Hippophae RHAMNOIDES) fruit oil uniformly according to formula, heating to 58 deg.C, adding unilateral flower (Orthilia SECUNDA) extract, stirring at 58 deg.C for 25min, stirring at 120rpm, cooling to 30 deg.C, filtering, inspecting, discharging, and packaging.
Comparative example 3
An anti-aging compound essential oil is prepared from the following raw materials in parts by weight: 45 parts of sunflower (Helianthus ANNUUS) seed oil, 50 parts of evening primrose (Oenothera BIENNIS) oil, 2 parts of ROSA rusticana (Rosa RUBIGINOSA) seed oil, 1.5 parts of sea buckthorn (Hippophae RHAMNOIDES) fruit oil, 0.5 part of Aucumaria KLAINEANA (Aucumea klainiana) resin extract, and 0.5 part of macadamia nut (MECADAMIA TERNIFOLIA) seed oil.
The preparation method of the anti-aging compound essential oil comprises the following steps: mixing semen Helianthi (Helianthus ANNUUS) oil, evening primrose (Oenothera BIENNIS) oil, Rosa RUBIGINOSA (Rosa RUBIGINOSA) seed oil and fructus Hippophae (Hippophae RHAMNOIDES) fruit oil uniformly according to formula, heating to 58 deg.C, adding Aucumea KLAINEANA (Aucumea klainea) resin extract and unilaterally flower (Orthilia SECUNDA) extract, stirring at 58 deg.C for 25min, stirring at 120rpm, cooling to 30 deg.C, filtering, inspecting, discharging, and packaging.
Comparative example 4
Essentially the same as example 2, except that: the Aucumman (Aucoumea KLAINEANA) resin extract is prepared by different methods.
The process for the preparation of the augustum resin extract in this example comprises the following steps:
s1, pretreatment: adding 1.5mol/L NaOH solution into the Oregamann resin, adjusting the pH value to 9, wherein the mass ratio of the Oregamann resin to the 1.5mol/L NaOH solution is 1: 9, stirring, mixing, standing for 20min, filtering, washing with water, and drying for later use;
s2, supercritical fluid extraction: extracting the Auguman resin treated in the step S1 by adopting a supercritical fluid extraction technology to obtain an extract liquid;
extraction conditions are as follows: extraction pressure: 10 MPa; the extraction temperature is as follows: 36 ℃; CO 22Flow rate: 10L/h; extraction time: 2 h; the entrainer is deionized water, the addition amount is 3 wt% of the Oregaman resin, and the entrainer flow is as follows: 0.3 mL/min;
s3, purifying the extract: adding the extract obtained in the step S2 into an ionic liquid, wherein the volume ratio of the extract to the ionic liquid is 10: 50, the ionic liquid is 1-propyl-3-methylimidazole tetrafluoroborate; introducing CO2，CO2Introducing at a speed of 1.5mL/min for 5min, oscillating, and stopping introducing CO2Standing for layering, separating liquid, collecting an organic layer, adding a drying agent for drying, wherein the mass ratio of the drying agent to the ionic liquid is 1: and 4, mixing the magnesium sulfate and anhydrous copper sulfate according to the mass ratio of 5:1, and filtering to obtain the Augusman resin extract.
Comparative example 5
Essentially the same as example 2, except that: the Aucumman (Aucoumea KLAINEANA) resin extract is prepared by different methods.
The process for the preparation of the augustum resin extract in this example comprises the following steps:
s1, pretreatment: adding 1.5mol/L NaOH solution into the Oregamann resin, adjusting the pH value to 9, wherein the mass ratio of the Oregamann resin to the 1.5mol/L NaOH solution is 1: 9, stirring, mixing, standing for 20min, filtering, washing with water, and drying for later use;
s2, supercritical fluid extraction: extracting the Auguman resin treated in the step S1 by adopting a supercritical fluid extraction technology to obtain an extract liquid;
extraction conditions are as follows: extraction pressure: 10 MPa; the extraction temperature is as follows: 36 ℃; CO 22Flow rate: 10L/h; extraction time: 2 h; the entrainer is ethyl acetate, the addition amount is 3 wt% of the Augusman resin, and the entrainer flow rate is as follows: 0.3 mL/min;
s3, purifying the extract: adding the extract obtained in step S2And (3) adding the extraction liquid to the ionic liquid, wherein the volume ratio of the extraction liquid to the ionic liquid is 10: 50, the ionic liquid is 1-propyl-3-methylimidazole tetrafluoroborate; introducing CO2，CO2Introducing at a speed of 1.5mL/min for 5min, oscillating, and stopping introducing CO2Standing for layering, separating liquid, collecting an organic layer, adding a drying agent for drying, wherein the mass ratio of the drying agent to the ionic liquid is 1: and 4, mixing the magnesium sulfate and anhydrous copper sulfate according to the mass ratio of 5:1, and filtering to obtain the Augusman resin extract.
Test example 1
Skin condition improvement assay: 70 female subjects with healthy skin are selected, the left half face of the female subject is smeared with a test sample, and the right half face of the female subject is not smeared with any substance and is used as a blank control. The experiment is 8 weeks, the skin of the testee is detected and analyzed by a skin detector at 1 week, 4 weeks and 8 weeks respectively, the improvement effect of the to-be-detected sample on the skin elasticity, fine wrinkles and skin roughness is evaluated, and the average value of the three tests at 1 week, 4 weeks and 8 weeks is taken.
TABLE 1 skin quality improvement test table
Figure BDA0002552767110000111
Note: "+" is increasing and "-" is decreasing.
The anti-aging compound essential oil provided by the invention can be used for obviously improving the fine lines and roughness of the skin, and the skin is more elastic. As can be seen from the comparison between the example 2 and the example 1 and the comparison examples 1-3, the invention simultaneously adds two high-activity extracts of the Oregamann resin extract and the unilaterally flower extract, has synergistic effect and mutual supplement, and on one hand, the anti-wrinkle active ingredient contained in the invention can stimulate cell metabolism and promote the regeneration of skin collagen elastic fibers, thereby improving the skin and preventing the generation of new wrinkles; on the other hand, the anti-wrinkle active ingredient is used as an antioxidant to prevent skin subcutaneous fat from being oxidized, enhance the activity of skin epidermis and dermal cells, avoid skin premature senility and further achieve the effects of anti-wrinkle and skin care.
Test example 2
And (3) measuring the anti-aging capability: the anti-aging ability is measured by using SOD activity as an index.
Grouping and administration of experimental animals:
80 Kunming clean-grade mice, half male and female, with weight (20 +/-4) g, provided by the Experimental animal center of Zhejiang university, were randomly divided into 8 groups of 10 mice each. Starting from 8:00 in the morning of the first day, the test samples to be tested were separately gavaged daily for experimental intervention, mice were weighed 1 time every 1 day, and the gavage amount (10g/kg) was adjusted accordingly according to body weight. The control group was also perfused with an equal amount of saline. After 1 month, the patient is killed by cutting off the head, the brain is washed with cold physiological saline, and the cerebral cortex is peeled off for later use.
Index measurement: the cerebral cortex is taken to be prepared into homogenate of 10 percent (mass percentage), and each index is measured according to the specification of an SOD test box.
TABLE 2 SOD Activity test Table
Figure BDA0002552767110000121
Compared with a blank group, the experimental result P of the test sample to be tested of the examples 1-2 and the comparative examples 1-5 is less than 0.05, and the significant difference exists. The anti-aging compound essential oil can obviously improve the SOD activity of the cerebral cortex of the mouse. As can be seen from comparison between the example 2 and the examples 1 and the comparative examples 1 to 3, the invention simultaneously adds two high-activity extracts of the Augustan resin extract and the unilaterally flower extract, has synergistic effect, further improves the SOD activity of the cerebral cortex of the mice, enhances the capability of an organism for removing free radicals and lightens the attack of the free radicals on the macromolecules of the biological membrane by improving the SOD activity of the cerebral cortex, thereby weakening the damage of excessive free radicals on the biological membrane, ensuring the structural and functional integrity of the cell membrane, delaying the occurrence of apoptosis and having obvious anti-aging effect.
The foregoing detailed description of the preferred embodiments of the invention has been presented. It should be understood that numerous modifications and variations could be devised by those skilled in the art in light of the present teachings without departing from the inventive concepts. Therefore, the technical solutions available to those skilled in the art through logic analysis, reasoning and limited experiments based on the prior art according to the concept of the present invention should be within the scope of protection defined by the claims.

Claims (2)

1. The anti-aging skin care product is characterized by being compound essential oil which is prepared from the following raw materials in parts by weight: 30-50 parts of sunflower seed oil, 30-50 parts of evening primrose oil, 0.5-5 parts of russet rose seed oil, 0.5-5 parts of sea buckthorn fruit oil, 0.5-5 parts of Augusman resin extract and 0.5-5 parts of unilatera flower extract;
the preparation method of the Oregaman resin extract comprises the following steps:
s1, pretreatment: adding 1-2mol/L NaOH solution into the Oregamann resin, adjusting the pH value to 8-10, wherein the mass ratio of the Oregamann resin to the 1-2mol/L NaOH solution is 1: (2-20), stirring, mixing, standing for 10-30min, filtering, washing with water, and drying for later use;
s2, supercritical fluid extraction: extracting the Auguman resin treated in the step S1 by adopting a supercritical fluid extraction technology;
extraction conditions are as follows: extraction pressure: 6-12 MPa; the extraction temperature is as follows: 32-40 ℃; CO 22Flow rate: 7-12L/h; extraction time: 1.5-2.5 h; the entrainer is 1-butyl-3-methylimidazole hexafluorophosphate, the addition amount is 1-5 wt% of the Oregaman resin, and the entrainer flow rate is as follows: 0.2-2 mL/min;
s3, purifying the extract: adding the extract obtained in the step S2 into an ionic liquid, wherein the volume ratio of the extract to the ionic liquid is 10: (30-70), wherein the ionic liquid is 1-propyl-3-methylimidazolium tetrafluoroborate; introducing CO2，CO2Introducing at a speed of 1-2mL/min for 3-10min, oscillating, and stopping introducing CO2Standing for layering, separating liquid, collecting an organic layer, adding a drying agent for drying, wherein the mass ratio of the drying agent to the ionic liquid is 1: (1-5), filtering to obtain the Oregamann resin extract;
the drying agent is formed by mixing magnesium sulfate and anhydrous copper sulfate according to the mass ratio of 5: 1.
2. The method of preparing the anti-aging skin care product of claim 1, comprising the steps of: mixing sunflower seed oil, evening primrose oil, rose hip seed oil and sea buckthorn fruit oil uniformly according to the formula, heating to 50-70 ℃, adding the Oregaman resin extract and the unilaterally flower extract, stirring for 10-30min at 50-70 ℃, stirring at the rotation speed of 100 plus 200rpm, cooling to 25-35 ℃, filtering, inspecting, discharging and packaging.
CN202010582275.6A 2020-06-23 2020-06-23 Anti-aging skin care product and preparation method thereof Active CN111616988B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202010582275.6A CN111616988B (en) 2020-06-23 2020-06-23 Anti-aging skin care product and preparation method thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202010582275.6A CN111616988B (en) 2020-06-23 2020-06-23 Anti-aging skin care product and preparation method thereof

Publications (2)

Publication Number Publication Date
CN111616988A CN111616988A (en) 2020-09-04
CN111616988B true CN111616988B (en) 2021-01-29

Family

ID=72267433

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202010582275.6A Active CN111616988B (en) 2020-06-23 2020-06-23 Anti-aging skin care product and preparation method thereof

Country Status (1)

Country Link
CN (1) CN111616988B (en)

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2757046B1 (en) * 1996-12-16 1999-03-05 Dior Christian Parfums USE OF AN OKOUME RESIN EXTRACT, IN THE COSMETIC AND PHARMACEUTICAL AREAS, ESPECIALLY DERMATOLOGICAL
RU2012152178A (en) * 2012-12-04 2014-06-10 Федеральное государственное учреждение науки Институт общей и экспериментальной биологии Сибирского отделения Российской академии наук (ФГБУН ИОЭБ СО РАН) METHOD FOR PRODUCING ANTI-OXIDATIVE AND ANTI-INFLAMMATORY ACTIVITY PRODUCT
CN103361181A (en) * 2013-07-19 2013-10-23 中国医学科学院药用植物研究所海南分所 Rose compound essential oil for resisting skin aging
CN107441021A (en) * 2017-08-14 2017-12-08 北京健旭康技术有限公司 Purposes of the northeast produce in cosmetics are prepared
CN109223623A (en) * 2018-11-23 2019-01-18 黄岳娇 A kind of formula of oil-control composition

Also Published As

Publication number Publication date
CN111616988A (en) 2020-09-04

Similar Documents

Publication Publication Date Title
CN108721161B (en) Hair-nourishing composition and preparation method of liposome thereof
CN109157447B (en) Application of cherokee rose fruit extract in whitening, moisturizing and anti-aging skin care product and preparation method thereof
CN110037981A (en) A kind of skin care item and the preparation method and application thereof
CN105496901A (en) Cosmetic composition with anti-aging effect as well as preparation method and application thereof
CN106580732A (en) Emulsion and preparation method thereof
CN113995693B (en) Essence composition of liposome with anti-inflammatory effect, and preparation method and application thereof
KR101817514B1 (en) New use of hesperetin
CN104586700A (en) Vitex negundo linn leaf extract and preparation method as well as application thereof
CN112043661B (en) Whitening aloe gel and preparation method thereof
KR20140145278A (en) Cosmetic composition containing Cimicifuga heracleifolia extract, Cornus officinalis extract and Geranium nepalense extract for skin convergence and elasticity effect
CN109512734A (en) Have effects that restore exterior-applied plant extract, cosmetics of elasticity of skin and gloss and preparation method thereof
CN111616988B (en) Anti-aging skin care product and preparation method thereof
CN113940896B (en) Anti-aging skin repair liquid containing vitamin A alcohol and preparation method thereof
CN114053186B (en) Long-acting moisturizing and whitening essence liquid containing roses and preparation method of essence liquid
CN107441340B (en) Hand and foot chap cream for relieving swelling, pain and itching and nourishing skin and preparation method thereof
CN105342966A (en) Skin care moisturizer containing hirudo nipponia and preparation method of skin care moisturizer
CN112842983B (en) Herbal emulsion for sensitive muscle repair and preparation method thereof
CN115414289A (en) Cordyceps flower cosmetics and preparation method thereof
JP5855949B2 (en) Keratin production promoter, hair dye and nail polish
CN110946770A (en) Acne-removing cream with liquid crystal structure and preparation method thereof
KR100431393B1 (en) Cosmetic Composition for the Inhibition of Sebum Secretion Containing the Extracts of the Fruits of Prunus Mume and Punica Granatum
CN112402309A (en) Multi-vitamin mineral essence cream and preparation method thereof
KR101154501B1 (en) A composition of eliminating keratin comprising saussurea involucratae extract
RO107545B1 (en) Cream for oily skin
KR102337422B1 (en) Cosmetic composition comprising jojoba oil for skin calming care and skin trouble improvement, and preparing method the same

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant
TR01 Transfer of patent right
TR01 Transfer of patent right

Effective date of registration: 20210329

Address after: 510000 4th floor, building 2, No. 39, huancun street, Maogang, Huangpu District, Guangzhou City, Guangdong Province (office only)

Patentee after: Guangzhou Xiyan Biotechnology Co.,Ltd.

Address before: Floor 8, building 5, biological industry Incubation Park, No.60 Keyuan Avenue, high tech Development Zone, Nanning, Guangxi 530000

Patentee before: Guangxi mandili Biotechnology Co.,Ltd.