CN111603429A - Traditional Chinese medicine skin care lotion with whitening and freckle removing effects and preparation method thereof - Google Patents
Traditional Chinese medicine skin care lotion with whitening and freckle removing effects and preparation method thereof Download PDFInfo
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/35—Ketones, e.g. benzophenone
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/67—Vitamins
- A61K8/678—Tocopherol, i.e. vitamin E
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/735—Mucopolysaccharides, e.g. hyaluronic acid; Derivatives thereof
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- A—HUMAN NECESSITIES
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- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
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- A61K2800/78—Enzyme modulators, e.g. Enzyme agonists
- A61K2800/782—Enzyme inhibitors; Enzyme antagonists
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- A—HUMAN NECESSITIES
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- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
Abstract
The invention discloses a traditional Chinese medicine skin moisturizer with whitening and freckle removing effects and a preparation method thereof. The skin care lotion is rich in active ingredients with whitening and freckle removing functions, such as phenols, flavonoids, polysaccharides, volatile oil, organic acids, triterpenoid saponins and the like. The invention also discloses a preparation method of the skin moisturizer, which is simple, convenient and is convenient to operate.
Description
Technical Field
The invention belongs to the field of traditional Chinese medicine skin care, and particularly relates to a formula of a traditional Chinese medicine skin care lotion with whitening and freckle removing effects and a preparation method thereof.
Background
In recent years, with the increasing aesthetic consciousness of people, people seek fair skin, the products with poor quality stimulate people to seek higher quality for beauty care, and the use of skin-whitening and freckle-removing skin care products to achieve bright and white skin becomes a common pursuit of many beauty lovers. In recent years, beauty and skin care products have raised the trend of returning to nature, and green, nontoxic and effective substances in the nature are taken as raw materials, so that the beauty effect is ensured, and meanwhile, the requirements of consumers are met by the products which are milder and safer.
Various types of spots appear on the skin surface, and the main cause is the deposition of melanin. Melanin is a high molecular biological pigment mainly distributed in epidermal cells and keratinocytes of a human body, and the formation process of the melanin is a series of complex physiological and biochemical processes, so that in order to achieve the effects of whitening and removing freckles, the generation of the melanin is inhibited from the source, and the migration or decomposition of the generated melanin is cut off. The formation of melanin is closely related to tyrosinase, which is the main rate-limiting enzyme in the process of melanin formation, and when melanin is excessively accumulated, problems such as color spots, freckles, skin darkness and the like can be caused. The method reduces influence on melanin generation by inhibiting the activity of tyrosinase, and is the most main way for whitening and removing freckles; in addition, free radicals are one of the factors influencing melanogenesis, and research shows that the reduction of melanogenesis can be realized by removing free radicals; for the generated melanin, a mode of blocking the melanin from being transported from dendrites of melanocytes to keratinocytes can be adopted, and the expression of epidermal melanin is reduced, so that the whitening effect is achieved, and the better whitening and freckle removing effects are achieved.
Disclosure of Invention
The invention aims to provide the skin care lotion which is composed of traditional Chinese medicine extracts and has the effects of whitening and removing freckles, and can safely and effectively fade facial spots and make skin have good color.
The invention also aims to provide a preparation method of the skin care lotion with the effects of whitening and removing freckles, which is composed of the traditional Chinese medicine extracts, can retain the active ingredients in the extracting solution to the greatest extent, and is simple and convenient to operate.
In order to achieve the purpose, the technical scheme of the invention is as follows:
the traditional Chinese medicine skin care lotion with the effects of whitening and removing freckles is prepared from the following compound traditional Chinese medicine components in parts by mass: 5-25% of liquorice extract, 5-25% of jasmine flower extract, 5-25% of catechu extract, 5-25% of poria cocos extract, 4-15% of roughhaired holly root extract, 4-15% of angelica dahurica extract, 4-12% of kelp extract, 4-12% of broussonetia papyrifera extract, 4-12% of duckweed extract, 4-12% of oroxylum indicum extract, 4-12% of lygodium japonicum extract, 4-12% of radix pseudostellariae extract, 4-12% of lophatherum gracile extract, 2-8% of mulberry bark extract, 2-8% of peach blossom extract, 2-8% of aster tataricus extract, 2-8% of fresh aloe juice, 2-8% of 95% ethanol, 1-5% of propylene, 1-5% of glycerin, 1-5% of astaxanthin, 1-5% of ethoxylated lanolin (lanolin polyoxyethylene ether), 1-5% of tocopherol acetate and 0.05-0.3% of essence, wherein liquorice, jasminum grandiflorum, catechu, poria cocos, holly, kelp, angelica dahurica, broussonetia papyrifera, duckweed, oroxylum indicum, sargentgloryvine stem, radix pseudostellariae, lophatherum gracile and white mulberry root bark are the core formula of the compound traditional Chinese medicine skin care lotion, and other components are auxiliary formulas.
By adopting the technical scheme, the invention relates to a traditional Chinese medicine skin care lotion with whitening and freckle removing effects, which has the following beneficial effects: the liquorice in the formula contains 3 major active ingredients: flavones, triterpenes (glycyrrhizic acid as main ingredient) and polysaccharides, which have effects of preventing skin aging, effectively eliminating superoxide ion and inhibiting tyrosinase activity; the jasminum grandiflorum is rich in flavonoids, phenols, triterpenes and saccharides, and can improve immunity of the organism, prevent skin aging and inhibit melanin generation; catechin rich in Catechu can regulate oxygen free radical system, improve organism oxidation resistance and relieve pigmentation; the imperatorin contained in radix Angelicae Dahuricae can inhibit tyrosinase activity in melanocyte or block conduction of melanin synthesis signal, and can be used for preventing and treating mottle and chloasma; the radix pseudostellariae contains various saponins, amino acids, arbutin, vitamins and other effective components, and has remarkable curative effects on freckles, chloasma, butterfly spot and senile skin pigmentation; tuckahoe, poria cocos: the pachyman can improve and enhance cellular immunity and promote metabolism, the pachyman and pachylon triterpene can enhance superoxide dismutase activity and reduce peroxidase activity, and have antioxidant and antiaging effects, and hydroxyproline can increase collagen fiber in skin and delay skin aging. Most of the medicinal materials used in the main formula are selected from genuine producing areas, and the whitening active ingredients are rich, so that the effect is better compared with that in common producing areas.
Preferably, the preparation method of the traditional Chinese medicine skin care lotion with the effects of whitening and removing freckles comprises the following steps:
(1) respectively cleaning licorice, jasminum grandiflorum, catechu, poria cocos, roughhaired holly root, kelp, angelica dahurica, broussonetia papyrifera, duckweed, oroxylum indicum, lygodium japonicum, radix pseudostellariae, lophatherum gracile, cortex mori radicis, peach blossom, radix asteris and other medicinal materials, repeatedly extracting for 2-4 times with deionized water (95-100 ℃) in a ratio of 1: 12-14, 2-4 hours each time, filtering, and combining to obtain water extract of each traditional Chinese medicine;
(2) adding 95% ethanol (material-liquid ratio 1: 7-10) into the residue of each Chinese medicinal material, soaking at 40-70 deg.C for 24-48 hr, filtering, and mixing the ethanol filtrate with the water extractive solution of step (1);
(3) respectively adding the combined extracting solution into a macroporous resin adsorption column for adsorption elution, wherein an eluant is an ethanol solution with the mass/volume percentage of 20-30%, the elution flow rate is 2mL/min, and collecting the eluted extracting solution;
(4) adding the extract of each Chinese medicine after elution into a sterile tank according to the weight percentage of the formula in claim 2, and simultaneously adding propylene glycol, sodium hyaluronate, glycerol, astaxanthin, ethoxylated lanolin (lanolin polyoxyethylene ether), tocopherol acetate, fresh aloe juice and essence into the sterile tank according to the weight percentage of the formula in claim 2, and aging for 24-48 hours;
(5) filtering the aged traditional Chinese medicine skin care solution by adopting ultrafiltration membrane filtering equipment, wherein the adopted ultrafiltration membrane filtering equipment is Bestriver UF-BRT-380, the filtering mode is internal pressure type, the operating pressure is 0.3MPa, the working temperature is 35 ℃, the filtering precision is 6-50nm, macromolecular impurities invisible to naked eyes are thoroughly separated, and the traditional Chinese medicine skin care solution is bottled after being inspected to be qualified.
After the technical scheme is adopted, the method is simple and convenient to operate, the prepared traditional Chinese medicine skin care lotion is clear and bright, the feed liquid in the filtering process is operated in an internal pressure type mode, no filter aid is added, the filtering process is operated at pure physical normal temperature, no chemical reaction exists, thermosensitive components are not damaged, the purity of the traditional Chinese medicine is improved, the traditional Chinese medicine skin care lotion is easy to absorb by the skin, and the generation of melanin is inhibited and the discharge of melanin is promoted through multiple ways, so that the effects of whitening and removing freckles are achieved.
Detailed Description
First, in order to further explain the technical solution of the present invention, the present invention is explained in detail by the following specific examples.
Example 1:
in the embodiment, the important medicinal materials selected in the main medicine formula are genuine medicinal materials.
Preferably, the licorice origin is the western inner Mongolia; the production place of the jasminum grandiflorum is Yunnan; the producing place of the catechu is the south of Yunnan; the origin of the tuckahoe is the Dabie mountain area in the north of east Hubei province; the production place of the roughhaired holly is Guangdong Meizhou; the production place of the oroxylum indicum is Fujian; the radix Pseudostellariae is Cudrania tricuspidata No. 1 of Cudrania tricuspidata county, Ningde City of Fujian province.
(1) Respectively cleaning Glycyrrhrizae radix, flos Jasmini sambac, Catechu, Poria, flos Ilicis Asprellae, thallus laminariae, radix Angelicae Dahuricae, radix Broussonetiae, herba Spirodelae, semen Oroxyli, caulis Sargentodoxae, radix Pseudostellariae, folium Bambusae, cortex Mori, flos persicae, and radix Asteris, extracting with deionized water (98 deg.C) at a ratio of 1: 13 repeatedly for 3 times, each for 3 hr, filtering, and mixing to obtain water extractive solution of each Chinese medicine.
(2) Soaking the residue of each Chinese medicinal material in 95% ethanol (material-liquid ratio of 1: 8) at 55 deg.C for 36 hr, filtering, and mixing the ethanol filtrate with the water extractive solution.
(3) And (3) respectively adding the combined extracting solution into a macroporous resin (consisting of SP207 and HP2MGL in a mass ratio of 3: 7) adsorption column for adsorption elution, wherein an eluent is an ethanol solution with the mass/volume percentage of 25%, the elution flow rate is 2mL/min, and collecting the eluted extracting solution.
(4) Weighing 10% of eluted liquorice extract, 8% of jasminum grandiflorum extract, 8% of catechu extract, 8% of poria cocos extract, 7% of roughhaired holly extract, 6% of kelp extract, 6% of angelica dahurica extract, 6% of broussonetia papyrifera extract, 5% of duckweed extract, 4% of oroxylum indicum extract, 4% of lygodium japonicum extract, 4% of radix pseudostellariae extract, 4% of lophatherum gracile extract, 3% of mulberry bark extract, 3% of peach blossom extract and 2% of radix asteris extract, adding 1% of propylene glycol, 1.8% of sodium hyaluronate, 1.15% of glycerol, 1% of astaxanthin, 1% of ethoxylated lanolin (lanolin polyoxyethylene ether), 1% of tocopherol acetate, 5% of fresh aloe juice and 0.05% of essence into an aseptic tank.
(5) The aged traditional Chinese medicine skin care solution adopts Bestriver UF-BRT-380 ultrafiltration membrane filtration equipment, under the conditions that the operating pressure is 0.3MPa, the working temperature is 35 ℃ and the filtration precision is 6-50nm, an internal pressure type filtration mode is adopted to thoroughly separate macromolecular impurities invisible to the naked eye, and the aged traditional Chinese medicine skin care solution is bottled after being inspected to be qualified. Sterilizing, and labeling to obtain the final product.
Example 2:
in the embodiment, the important medicinal materials selected in the main medicine formula are genuine medicinal materials.
Preferably, the licorice origin is the western inner Mongolia; the production place of the jasminum grandiflorum is Yunnan; the producing place of the catechu is the south of Yunnan; the origin of the tuckahoe is the Dabie mountain area in the north of east Hubei province; the production place of the roughhaired holly is Guangdong Meizhou; the production place of the oroxylum indicum is Fujian; the radix Pseudostellariae is Cudrania tricuspidata No. 1 of Cudrania tricuspidata county, Ningde City of Fujian province.
(1) Respectively cleaning Glycyrrhrizae radix, flos Jasmini sambac, Catechu, Poria, flos Ilicis Asprellae, thallus laminariae, radix Angelicae Dahuricae, radix Broussonetiae, herba Spirodelae, semen Oroxyli, caulis Sargentodoxae, radix Pseudostellariae, folium Bambusae, cortex Mori, flos persicae, and radix Asteris, extracting with deionized water (98 deg.C) at a ratio of 1: 13 repeatedly for 3 times, each for 3 hr, filtering, and mixing to obtain water extractive solution of each Chinese medicine.
(2) Soaking the residue of each Chinese medicinal material in 95% ethanol (material-liquid ratio of 1: 8) at 55 deg.C for 36 hr, filtering, and mixing the ethanol filtrate with the water extractive solution. .
(3) And (3) respectively adding the combined extracting solution into a macroporous resin (consisting of SP207 and HP2MGL in a mass ratio of 3: 7) adsorption column for adsorption elution, wherein an eluent is an ethanol solution with the mass/volume percentage of 25%, the elution flow rate is 2mL/min, and collecting the eluted extracting solution.
(4) Weighing 8% of eluted liquorice extract, 7% of jasminum grandiflorum extract, 7% of catechu extract, 6% of poria cocos extract, 6% of roughhaired holly extract, 7% of kelp extract, 7% of angelica dahurica extract, 4% of broussonetia papyrifera extract, 6% of duckweed extract, 5% of oroxylum indicum extract, 5% of lygodium japonicum extract, 5% of radix pseudostellariae extract, 4% of lophatherum gracile extract, 4% of cortex mori extract, 4% of peach blossom extract and 3% of radix asteris extract according to weight percentage, adding the raw materials into an aseptic tank, and simultaneously adding 1% of propylene glycol, 1.8% of sodium hyaluronate, 1.15% of glycerol, 1% of astaxanthin, 1% of ethoxylated lanolin (lanolin polyoxyethylene ether), 1% of tocopherol acetate, 5% of fresh.
(5) The aged traditional Chinese medicine skin care solution adopts Bestriver UF-BRT-380 ultrafiltration membrane filtration equipment, under the conditions that the operating pressure is 0.3MPa, the working temperature is 35 ℃ and the filtration precision is 6-50nm, an internal pressure type filtration mode is adopted to thoroughly separate macromolecular impurities invisible to the naked eye, and the aged traditional Chinese medicine skin care solution is bottled after being inspected to be qualified. Sterilizing, and labeling to obtain the final product.
Example 3:
in the embodiment, the important medicinal materials selected in the main medicine formula are genuine medicinal materials.
Preferably, the licorice origin is the western inner Mongolia; the production place of the jasminum grandiflorum is Yunnan; the producing place of the catechu is the south of Yunnan; the origin of the tuckahoe is the Dabie mountain area in the north of east Hubei province; the production place of the roughhaired holly is Guangdong Meizhou; the production place of the oroxylum indicum is Fujian; the radix Pseudostellariae is Cudrania tricuspidata No. 1 of Cudrania tricuspidata county, Ningde City of Fujian province.
(1) Respectively cleaning Glycyrrhrizae radix, flos Jasmini sambac, Catechu, Poria, flos Ilicis Asprellae, thallus laminariae, radix Angelicae Dahuricae, radix Broussonetiae, herba Spirodelae, semen Oroxyli, caulis Sargentodoxae, radix Pseudostellariae, folium Bambusae, cortex Mori, flos persicae, and radix Asteris, extracting with deionized water (98 deg.C) at a ratio of 1: 13 repeatedly for 3 times, each for 3 hr, filtering, and mixing to obtain water extractive solution of each Chinese medicine.
(2) Soaking the residue of each Chinese medicinal material in 95% ethanol (material-liquid ratio of 1: 8) at 55 deg.C for 36 hr, filtering, and mixing the ethanol filtrate with the water extractive solution.
(3) And (3) respectively adding the combined extracting solution into a macroporous resin (consisting of SP207 and HP2MGL in a mass ratio of 3: 7) adsorption column for adsorption elution, wherein an eluent is an ethanol solution with the mass/volume percentage of 25%, the elution flow rate is 2mL/min, and collecting the eluted extracting solution.
(4) Weighing 5% of eluted liquorice extract, 5% of jasminum grandiflorum extract, 5% of catechu extract, 5% of poria cocos extract, 7% of roughhaired holly extract, 7% of kelp extract, 8% of angelica dahurica extract, 6% of broussonetia papyrifera extract, 6% of duckweed extract, 6% of oroxylum indicum extract, 4% of lygodium japonicum extract, 4% of radix pseudostellariae extract, 4% of lophatherum gracile extract, 6% of cortex mori extract, 5% of peach blossom extract and 5% of radix asteris extract according to weight percentage, adding the raw materials into an aseptic tank, and simultaneously adding 1% of propylene glycol, 1.8% of sodium hyaluronate, 1.15% of glycerol, 1% of astaxanthin, 1% of ethoxylated lanolin (lanolin polyoxyethylene ether), 1% of tocopherol acetate, 5% of fresh.
(5) The aged traditional Chinese medicine skin care solution adopts Bestriver UF-BRT-380 ultrafiltration membrane filtration equipment, under the conditions that the operating pressure is 0.3MPa, the working temperature is 35 ℃ and the filtration precision is 6-50nm, an internal pressure type filtration mode is adopted to thoroughly separate macromolecular impurities invisible to the naked eye, and the aged traditional Chinese medicine skin care solution is bottled after being inspected to be qualified. Sterilizing, and labeling to obtain the final product.
Example 4:
in this example, all herbs were selected from the general production area.
(1) Respectively cleaning Glycyrrhrizae radix, flos Jasmini sambac, Catechu, Poria, flos Ilicis Asprellae, thallus laminariae, radix Angelicae Dahuricae, radix Broussonetiae, herba Spirodelae, semen Oroxyli, caulis Sargentodoxae, radix Pseudostellariae, folium Bambusae, cortex Mori, flos persicae, and radix Asteris, extracting with deionized water (98 deg.C) at a ratio of 1: 13 repeatedly for 3 times, each for 3 hr, filtering, and mixing to obtain water extractive solution of each Chinese medicine.
(2) Soaking the residue of each Chinese medicinal material in 95% ethanol (material-liquid ratio of 1: 8) at 55 deg.C for 36 hr, filtering, and mixing the ethanol filtrate with the water extractive solution.
(3) And (3) respectively adding the combined extracting solution into a macroporous resin (consisting of SP207 and HP2MGL in a mass ratio of 3: 7) adsorption column for adsorption elution, wherein an eluent is an ethanol solution with the mass/volume percentage of 25%, the elution flow rate is 2mL/min, and collecting the eluted extracting solution.
(4) Weighing 10% of eluted liquorice extract, 8% of jasminum grandiflorum extract, 8% of catechu extract, 8% of poria cocos extract, 7% of roughhaired holly extract, 6% of kelp extract, 6% of angelica dahurica extract, 6% of broussonetia papyrifera extract, 5% of duckweed extract, 4% of oroxylum indicum extract, 4% of lygodium japonicum extract, 4% of radix pseudostellariae extract, 4% of lophatherum gracile extract, 3% of mulberry bark extract, 3% of peach blossom extract and 2% of radix asteris extract, adding 1% of propylene glycol, 1.8% of sodium hyaluronate, 1.15% of glycerol, 1% of astaxanthin, 1% of ethoxylated lanolin (lanolin polyoxyethylene ether), 1% of tocopherol acetate, 5% of fresh aloe juice and 0.05% of essence into an aseptic tank.
(5) The aged traditional Chinese medicine skin care solution adopts Bestriver UF-BRT-380 ultrafiltration membrane filtration equipment, under the conditions that the operating pressure is 0.3MPa, the working temperature is 35 ℃ and the filtration precision is 6-50nm, an internal pressure type filtration mode is adopted to thoroughly separate macromolecular impurities invisible to the naked eye, and the aged traditional Chinese medicine skin care solution is bottled after being inspected to be qualified. Sterilizing, and labeling to obtain the final product.
And secondly, irritation and allergy tests of the traditional Chinese medicine skin care lotion.
(1) Experimental animals: 50 Kunming white mice were randomly divided into 5 groups, a blank group and 10 groups of examples 1, 2, 3 and 4, and the back hair of the mice was shaved with a shaver.
(2) The experimental process comprises the following steps: the back of the blank group of mice was smeared with physiological saline, and the mice of the example group were smeared with the prepared skin moisturizers, and continuously observed for one week.
(3) The experimental results are as follows: by comparing the blank group mice with the test group mice, no irritation or anaphylactic reaction is found, which indicates that the skin lotion has no irritation or anaphylaxis.
And thirdly, the samples prepared in the examples measure the tyrosinase inhibition rate and the DPPH free radical clearance rate.
(I) determination of tyrosinase inhibition:
1. preparing a solution:
(1) preparation of Phosphate Buffered Saline (PBS): respectively and accurately weighing 71.6g of disodium hydrogen phosphate and 31.2g of sodium dihydrogen phosphate, adding deionized water to fully dissolve, respectively transferring to a 1000mL measuring flask to constant volume to prepare 0.2 mol.L～1The solution of (4) is mixed uniformly according to the proportion of 51: 49, and the pH is adjusted to 6.8, thus obtaining the product.
(2) Preparation of tyrosinase solution: accurately weighing a certain amount of 920 U.mg～1Tyrosinase, dissolved in PBS to 200 U.mg～1The enzyme solution is preserved and frozen in a refrigerator at the subzero temperature environment.
(3) Preparing a dopa solution: precisely weighing a certain amount of L-dopamine, and adding a small amount of 0.1 mol.L-1Pre-dissolving in hydrochloric acid solution, diluting with PBS to 1.5 g.L～1Dopa solution, stored at 4 ℃ in the dark.
(4) And diluting the skin care lotion obtained in the examples 1-4 by 2 times by using PBS to obtain a sample to be detected.
2. Measurement method
Respectively adding PBS, a sample to be detected and a tyrosinase solution into each test tube according to the design of table 1, uniformly mixing, reacting in a constant-temperature water bath (37 ℃) for 10min, respectively and rapidly transferring into 1mL of dopa solution, uniformly mixing, placing in a water bath for reacting for 5min, and immediately detecting A when the reaction reaches 5minλWhen the test piece is 475, A is measured1、A2、A3And A4The test was performed in parallel 3 times. Calculating according to the formula:
TABLE 1 composition of the reaction solution
(II) determination of DPPH radical scavenging Rate:
1. preparing a solution:
(1) accurately weighing a certain amount of DPPH, adding absolute ethyl alcohol to dissolve the DPPH to prepare the product with the mass concentration of about 0.04 g.L～1The DPPH solution is stored at 4 ℃ in the dark for later use.
(2) The skin lotions obtained in examples 1 to 4 were diluted 100 times with ethanol (95%) to obtain samples to be tested.
2. The determination method comprises the following steps:
precisely transferring the DPPH free radical solution and absolute ethyl alcohol into the same test tube with 2mL each, uniformly mixing, reacting for 20min in a dark place, and measuring the absorbance A of lambda-5171. Precisely transferring 2mL of each of the DPPH free radical solution and the sample solution to be detected into the same test tube, uniformly mixing, reacting for 20min in a dark place, and measuring the absorbance A of lambda (517)2(ii) a Precisely transferring 2mL of ethanol and sample solution to be detected into the same test tube, mixing, standing in dark for 20min, and measuring lambda-517 absorbance A3.3 replicates were performed. The DPPH clearance rate of the test liquid medicine is calculated according to a formula:
(III) results:
as can be seen from table 2, the skin care lotion with whitening and spot removing effects prepared in example 1 has the highest tyrosinase inhibition rate, while the DPPH clearance rate in example 2 is slightly higher than that in examples 1, 3 and 4, and the whitening effect in example 1 is the best in comprehensive consideration. Wherein the tyrosinase inhibition rate and DPPH clearance rate of example 1 using genuine herbs are higher than those of example 4 using genuine herbs, compared with example 1 where the Chinese herbal medicine components have the same specific gravity.
Table 2 results of skin lotions according to different examples
Note: the overall score is tyrosinase inhibition x 50% + DPPH clearance x 50%.
Claims (4)
1. The traditional Chinese medicine skin care lotion with the effects of whitening and removing freckles is characterized by comprising the following components: licorice, jasminum grandiflorum, catechu, poria cocos, roughhaired holly, kelp, angelica dahurica, broussonetia papyrifera, duckweed, oroxylum indicum, lygodium japonicum, radix pseudostellariae, lophatherum gracile, cortex mori, peach blossom, aster, aloe, 95% ethanol, propylene glycol, sodium hyaluronate, glycerol, astaxanthin, ethoxylated lanolin (lanolin polyoxyethylene ether), tocopherol acetate and essence.
2. The traditional Chinese medicine skin care lotion with whitening and freckle removing effects according to claim 1 is characterized in that:
the core components and the weight percentage are as follows: 5-25% of liquorice extract, 5-25% of jasmine flower extract, 5-25% of catechu extract, 5-25% of poria cocos extract, 4-15% of roughhaired holly extract, 4-15% of angelica dahurica extract, 4-12% of kelp extract, 4-12% of broussonetia papyrifera extract, 4-12% of duckweed extract, 4-12% of oroxylum indicum extract, 4-12% of lygodium japonicum extract, 4-12% of radix pseudostellariae extract, 4-12% of lophatherum gracile extract and 2-8% of cortex mori extract.
The auxiliary components and the weight percentage are as follows: 2 to 8 percent of peach blossom extracting solution, 2 to 8 percent of aster extracting solution, 2 to 8 percent of fresh aloe juice, 2 to 8 percent of 95 percent ethanol, 1 to 5 percent of propylene glycol, 1 to 5 percent of sodium hyaluronate, 1 to 5 percent of glycerol, 1 to 5 percent of astaxanthin, 1 to 5 percent of ethoxylated lanolin (lanolin polyoxyethylene ether), 1 to 5 percent of tocopherol acetate and 0.05 to 0.3 percent of essence.
3. A preparation method of a traditional Chinese medicine skin care lotion with whitening and freckle removing effects is characterized by comprising the following steps: the preparation method comprises the following specific steps:
(1) respectively cleaning licorice, jasminum grandiflorum, catechu, poria cocos, roughhaired holly root, kelp, angelica dahurica, broussonetia papyrifera, duckweed, oroxylum indicum, lygodium japonicum, radix pseudostellariae, lophatherum gracile, cortex mori radicis, peach blossom, radix asteris and other medicinal materials, repeatedly extracting for 2-4 times with deionized water (95-100 ℃) in a ratio of 1: 12-14, 2-4 hours each time, filtering, and combining to obtain water extract of each traditional Chinese medicine;
(2) adding 95% ethanol (material-liquid ratio 1: 7-10) into the residue of each Chinese medicinal material, soaking at 40-70 deg.C for 24-48 hr, filtering, and mixing the ethanol filtrate with the water extractive solution of step (1);
(3) respectively adding the combined extracting solution into a macroporous resin adsorption column for adsorption elution, wherein an eluant is an ethanol solution with the mass/volume percentage of 20-30%, the elution flow rate is 2mL/min, and collecting the eluted extracting solution;
(4) adding the extract of each Chinese medicine after elution into a sterile tank according to the weight percentage of the formula in claim 2, and simultaneously adding propylene glycol, sodium hyaluronate, glycerol, astaxanthin, ethoxylated lanolin (lanolin polyoxyethylene ether), tocopherol acetate, fresh aloe juice and essence into the sterile tank according to the weight percentage of the formula in claim 2, and aging for 24-48 hours;
(5) filtering the aged traditional Chinese medicine skin care solution by adopting ultrafiltration membrane filtering equipment, wherein the adopted ultrafiltration membrane filtering equipment is Bestriver UF-BRT-380, the filtering mode is internal pressure type, the operating pressure is 0.3MPa, the working temperature is 35 ℃, the filtering precision is 6-50nm, macromolecular impurities invisible to naked eyes are thoroughly separated, and the traditional Chinese medicine skin care solution is bottled after being inspected to be qualified.
4. The preparation method of the traditional Chinese medicine skin care lotion with whitening and freckle removing effects according to claim 3, which is characterized by comprising the following steps of: the macroporous resin of the macroporous resin adsorption column used in the preparation process is formed by mixing two types of resins, namely SP207 and HP2MGL, and the mass ratio is as follows: SP 207: HP2MGL is 3: 7.
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