CN111551417B

CN111551417B - Automatic film-making device

Info

Publication number: CN111551417B
Application number: CN202010249420.9A
Authority: CN
Inventors: 詹晓春; 付凤霞; 詹斯喻
Original assignee: Zhengzhou Zhongpu Medical Equipment Co ltd
Current assignee: Zhengzhou Zhongpu Medical Equipment Co ltd
Priority date: 2020-04-01
Filing date: 2020-04-01
Publication date: 2023-08-11
Anticipated expiration: 2040-04-01
Also published as: CN111551417A

Abstract

The application relates to an automatic sheet making device which comprises a mold cup feeding device and a smear mechanism, wherein the mold cup feeding device comprises a mold cup placing frame, a mold cup pushing mechanism, a mold cup discharging mechanism and a mold cup transferring mechanism; the mould cup placing frame comprises a frame which can move linearly and is used for placing the mould cups in a pile, and the frame sends the mould cups to the mould cup pushing mechanism; the mold cup pushing mechanism enables one mold cup to drop at a time; the mold cup transferring mechanism comprises a transferring seat, a clamping hole for placing the mold cup is arranged on the transferring seat, and the transferring seat transfers the mold cup away from the material stirring mechanism; the smear mechanism comprises a smear frame, wherein two liftable mold cup rods are arranged on the smear frame and are used for inserting and taking mold cups in the transfer seat, and the mold cups stained with the sample liquid are sent to a slide placing table to smear a slide on the slide placing table. The manual participation degree is reduced, the automation level is improved, the efficiency is improved, the operation is more standard, and the difference caused by different people is avoided.

Description

Automatic film-making device

Technical Field

The application relates to the technical field of slide preparation and dyeing, in particular to an automatic slide preparation device.

Background

Automation and intellectualization are the development directions of medical laboratories in the future. However, most hospitals and laboratories at present have the problems of low efficiency and high risk in the aspect of processing patient samples for detection. The links of film making, dyeing, scanning and the like are respectively carried out by adopting different instruments, and the manual operation has more steps, so that the efficiency is low, larger artificial difference results can be generated when different people operate the film making, dyeing and scanning, and the accuracy of the result is also affected to a certain extent. For sample detection like tuberculosis patients, the risk of infection of other related personnel may also be caused by the artificial manipulation due to the infectivity of tuberculosis.

There are also some automation devices, but most of them focus on the optimization design of each link, and there is no very effective full-flow automation device. For example, an automatic constant temperature high definition dyeing device disclosed in the patent of publication No. CN110286023A replaces an artificial dyeing and low-efficiency dyeing instrument, and can automatically dye a plurality of slides at one time, thereby improving the efficiency. Another example is a slide magazine module and microscope scanning system with the module as disclosed in the patent publication CN 207133501U. And a corresponding high-automation tabletting device is also lacking.

Disclosure of Invention

The application aims to provide an automatic film-making device which is used for improving the automation degree of a film-making link and reducing the manual participation degree.

In order to achieve the above purpose, the application adopts the following technical scheme: an automatic film-making device comprises a mold cup supply device and a smear mechanism, wherein the mold cup supply device comprises a mold cup placing frame, a mold cup pushing mechanism, a mold cup blanking mechanism and a mold cup transferring mechanism; the mould cup placing frame comprises a frame which can move linearly and is used for placing the mould cups in a pile, and the frame sends the mould cups to the mould cup pushing mechanism; the die cup pushing mechanism comprises a die cup pushing plate which moves linearly to push the piled die cups into the die cup blanking mechanism; the die cup blanking mechanism comprises a die cup positioning frame and a stirring mechanism, wherein the die cup positioning frame is positioned above the stirring mechanism and used for positioning a pushed die cup, the stirring mechanism comprises a stirring block capable of intermittently rotating or a stirring rod capable of intermittently stretching, the stirring block or the stirring rod supports the lowest die cup, and one die cup can drop at a time in the process of rotating or stretching the stirring rod; the mold cup transferring mechanism comprises a transferring seat, a clamping hole for placing the mold cup is arranged on the transferring seat, and the transferring seat transfers the mold cup away from the material stirring mechanism; the smear mechanism comprises a smear frame capable of horizontally moving, wherein two liftable mold cup rods are arranged on the upper part of the smear frame and are used for inserting and taking mold cups in the transfer seat, and the mold cups dipped with the sample liquid are sent to a slide placing table to smear slides on the slide placing table.

Further, more than two rows of mold cups are arranged in the frame, the mold cups in each row are sleeved together up and down, the upper part of each mold cup is provided with a cup edge, a support bar is arranged at the bottom in the frame, and the cup edge of the lowest mold cup is supported.

Further, a sliding block is arranged at the bottom of the frame and is driven by a screw nut mechanism.

Further, the die cup positioning frame is a U-shaped frame, and an opening faces one side of the die cup pushing plate; or the mold cup positioning frame is provided with more than two U-shaped openings so as to correspond to each stack of mold cups.

Further, the stirring mechanism comprises a driving stirring shaft and a driven stirring shaft, the axes of the driving stirring shaft and the driven stirring shaft are parallel, a driving stirring wheel and a driven stirring wheel are respectively arranged on the driving stirring shaft and the driven stirring shaft, the driving stirring wheel and the driven stirring wheel are in meshed transmission, one stirring block is respectively arranged at the end parts of the driving stirring shaft and the driven stirring shaft, the two stirring blocks are oppositely arranged and are used for supporting the lowest mold cup, the stirring blocks can intermittently rotate, and one mold cup can drop at a time in the rotating process of the stirring blocks.

Further, the driving thumb wheel and the driven thumb wheel are sector gears.

Further, the smear frame is driven by a corresponding screw nut mechanism, a supporting arm is arranged at the upper part of the smear frame, and a mold cup rod is arranged on the supporting arm and can move up and down; the smear frame is provided with a die withdrawing device, the die withdrawing device comprises a die withdrawing plate arranged on a guide rod, a die cup rod supporting seat for supporting a die cup rod is arranged on the upper portion of the die withdrawing plate, the die cup rod supporting seat is positioned above the die withdrawing plate, the die cup rod supporting seat is assembled on the guide rod in a guiding way, and a spring is arranged between the die withdrawing plate and the die cup rod supporting seat.

Further, a waste bin is further provided on one side of the smear frame for collecting the used mold cups.

Further, a sample bin is further arranged on one side of the transfer seat and used for temporarily storing sample liquid for being dipped by the mould cup.

The application has the beneficial effects that:

the automatic film-making device can realize that piled mold cups are conveyed to the transfer seat one by one under the action of the pushing mechanism, the pushing mechanism and the blanking mechanism, the transfer seat moves away from the blanking mechanism for inserting, taking and transferring mold cup rods in the film-making mechanism to a sample bin, the mold cup rods descend to enable the mold cups at the lower ends to be stained with sample liquid in the sample bin, ascend to leave and to a slide placing table, descend to contact with slides, and the sample liquid can be coated on the slides for film-making. In the whole process, the automatic mold cup placing device is automatic, manual intervention is not needed, and when the disposable mold cup is used, an operator puts a new mold cup into the mold cup placing frame.

Drawings

FIG. 1 is a schematic diagram of the appearance of a fully automated dye scanning system;

FIG. 2 is a schematic illustration of the fully automated dye scanning system of FIG. 1 with a portion of the housing removed;

FIG. 3 is a schematic view of a portion of the structure of FIG. 2;

FIG. 4 is a schematic view of the sample bottle delivery apparatus of FIG. 3;

FIG. 5 is a schematic view of the sample bottle holder of FIG. 4;

FIG. 6 is a schematic view of FIG. 4 with a portion of the sample vial rack removed;

FIG. 7 is a schematic view of the sample holder of FIG. 6;

FIG. 8 is another angular schematic view of FIG. 3;

FIG. 9 is a schematic view of a portion of the structure of FIG. 8;

FIG. 10 is a schematic view of FIG. 9 with portions of the shell plate removed;

FIG. 11 is a schematic view of the other angle of FIG. 10 (schematic view after removal of a portion of the mechanism);

FIG. 12 is a schematic view of the kick-out mechanism of FIG. 11;

FIG. 13 is a schematic view of the mechanism of FIG. 3 with portions removed;

FIG. 14 is a schematic view of the fully automated dye scanner system with the outside housing and portions of the mechanism removed; the method comprises the steps of carrying out a first treatment on the surface of the

Fig. 15 is a schematic view of the slide pushing device of fig. 14;

FIG. 16 is another angular schematic view of the slide pushing device;

FIG. 17 is a schematic view of FIG. 16 with portions of the outer panel cut away;

FIG. 18 is a schematic view of the slide placement frame of FIG. 17;

fig. 19 is a schematic view of the configuration of the slide pushing device in cooperation with the robot device;

FIG. 20 is a schematic view of the robot assembly of FIG. 19 (with a slide sandwiched therebetween);

FIG. 21 is a schematic view of the structure of the staining apparatus (with a slide inserted therein);

FIG. 22 is a schematic view of the cut-away structure of FIG. 21;

FIG. 23 is a schematic view of FIG. 22 with the slide removed;

FIG. 24 is a schematic view of a scanning device;

FIG. 25 is a schematic view of the structure of the slide automatic alignment device;

FIG. 26 is a schematic view of the glass stage of FIG. 25;

fig. 27 is a schematic view of a part of the structure of the slide storage device in fig. 14.

Name corresponding to each label in the figure:

1. the sample bottle conveying device comprises a shell, 10, a shielding plate, 2, a sample bottle conveying device, 20, a sample bottle input mechanism, 200, an input base, 201, a sample bottle rack, 2011, a guide clamping groove, 2012, a protruding part, 2013, a sample bottle positioning jack, 2014, a positioning notch, 202, a sample bottle, 203, a guide rail, 204, an input driving shaft, 21, a sample bottle output mechanism, 210, an output base, 22, a sample rack transfer mechanism, 220, a third servo motor, 221, a grabbing plate, 222 and a third lead screw; 30. the device comprises a sampling seat 301, a sampling groove 31, a second lead screw 32, a second servo motor 33 and a sample bin; 34. a scanning module; 4. a slide making device, 40, a mold cup pushing mechanism, 401, a pushing screw, 41, a material pulling mechanism, 411, a fifth servo motor, 412, a pulling block, 413, a driving pulling shaft, 414, a driven pulling shaft, 415, a driven pulling wheel, 42, a mold cup transferring mechanism, 421, a sixth servo motor, 422, a sixth screw, 423, a transferring seat, 4231, a clamping hole, 43, a frame, 431, a supporting bar, 432, a sliding block, 44, a fourth screw, 45, a mold cup positioning frame, 46, a fourth servo motor, 47, a smear mechanism, 471, a smear frame, 472, a mold cup rod, 473, a mold returning plate, 474, 475, a waste bin, 476, a seventh screw, 478, a spring, 479, a guide rod, 48, a slide placing table, 5, a slide pushing device, 51, a pushing frame, 511, a slide outlet, 512, a bayonet, 513, an inclined surface, 521, an eighth screw, 522, a slide elevating seat, 523, an eighth servo motor, 531, ninth servo motor, 532, ninth screw, 533, slide block, 5331, pushing section, 54, slide holding frame, 541, positioning boss, 6, robot device, 61, X-axis mechanism, 611, X-axis screw, 62, Z-axis mechanism, 621, Z-axis moving frame, 622, Z-axis screw, 63, Y-axis mechanism, 631, Y-axis moving frame, 632, Y-axis screw, 64, rotation mechanism, 641, gripper, 642, rotating arm, 7, dyeing device, 71, dyeing bin body, 72, liquid inlet and outlet hole, 73, overflow preventing port, 74, dyeing liquid bottle, 75, weighing module, 8, scanning device, 81, scanning microscope, 82, slide automatic alignment device, 821, up-down alignment mechanism, 8211, fixed bracket, 8212, tenth servo motor, 8213, tenth screw, 8214, up-down movement seat, 822, left-right screw nut mechanism, 8221, eleventh servo motor, 8222. the device comprises a supporting seat, 8223, an eleventh lead screw, 8224, a sliding seat, 83, a glass carrying table, 831, a slide positioning groove, 8311, a through hole, 8312, a positioning step surface, 8313, a yielding port, 832 and a slide buffer groove; A. and (3) a mold cup, B and a glass slide.

Detailed Description

The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present application.

Embodiments of the application:

in order to better illustrate and understand the automatic film making device of the application, the following description is presented by taking the whole full-automatic dyeing scanning system applied by the device as an introduction object, and describes the mechanism of an upstream link and a downstream link matched with the automatic film making device.

As shown in fig. 1 to 27, the full-automatic staining scanning system includes a sample bottle transporting apparatus 2, a slide producing apparatus 4, a staining apparatus 7, a slide storage apparatus 9, and a scanning apparatus 8, which are described in detail below. Each part is arranged in the shell 1 to form a sampling, tabletting, dyeing and scanning integrated machine.

As shown in fig. 2 to 7, the sample bottle transporting device 2 includes a sample bottle input mechanism 20 and a sample bottle output mechanism 21. The sample bottle input mechanism 20 comprises an input base 200, an input driving shaft 204 is arranged on the input base 200, the input driving shaft 204 is driven by a first servo motor through a belt wheel and a synchronous belt, two ends of the input driving shaft are rotatably assembled on the input base 200, two ends of the input driving shaft are respectively provided with an input thumb wheel (not shown in the figure), the input thumb wheels are positioned in the input base, and a plurality of thumb levers or thumb sheets are arranged on one circle of the input thumb wheels.

As shown in fig. 6, the upper part of the input base 200 is provided with guide rails 203 on both sides. A plurality of sample bottle racks 201 are arranged in a row on the input base, sample bottle positioning jacks 2013 are arranged on the sample bottle racks 201, and taper holes can be formed for the sample bottles 202 to be inserted. 4 sample bottles can be arranged on one sample bottle rack, the sample bottles are manually placed after sample liquid is collected, a corresponding push-pull shielding plate 10 is arranged on the bottom of the front side of the shell, and the sample bottles are required to be manually taken and placed. The sample bottle is used for containing a sample liquid to be detected. The bottom of the sample bottle is made of rubber and other software materials, and can be inserted by a sampling needle.

As the input thumbwheel rotates, the thumbstick can toggle and advance the sample bottle holder 201 along the guide rail 203 of the input base. As shown in fig. 5, a guiding slot 2011 is provided on the left and right sides of the sample bottle rack 201, and slidably engaged with the guiding rail 203. According to the set time interval, after the sample solution of the sample bottle on the innermost sample bottle rack is extracted and played, the next outermost sample bottle rack is pushed to move, each sample rack is sequentially pushed, and the innermost sample rack is positioned at the position to be extracted.

The sampling device is arranged below the input base 200 and comprises a sampling seat 30, two sampling grooves 301 are arranged in the sampling seat, and the sampling grooves respectively correspond to the two sample bottles 202. The sampling slots are each provided with a sampling needle (which extends out from the bottom, not shown).

As shown in fig. 7, the sampling base 30 is driven to be movable up and down by a screw nut mechanism, which is referred to as a sampling base screw nut mechanism, and includes a screw, which is referred to as a second screw 31, and a second servo motor 32 for driving the second screw. When sampling is desired, the sampling seat 30 is raised and a sampling needle is inserted into the bottom of the sample bottle 202. The sampling needle is connected to a fluid line and pump (not shown) to draw and deliver the sample into the sample chamber for tableting.

The sampling base 30 can sample two sample bottles at a time. After taking two, the sample bottle rack can move left under the action of the sample rack transfer mechanism 22, so that the other two sample bottles in one row are positioned at the position to be sampled. After all four sample bottles on the sample rack are sampled, the sample bottle rack continues to move left and is withdrawn outwards under the action of the sample bottle output mechanism 21.

The sample rack transfer mechanism 22 includes a horizontally disposed screw-nut mechanism, denoted transfer screw-nut mechanism, including a third servo motor 220 that drives the screw (third screw). The two arm plates at two sides of the grabbing plate 221 are provided with grabbing grooves which are just matched with protruding parts 2012 at two ends of the sample rack, the grabbing grooves are U-shaped grooves, and openings of the two grabbing grooves are opposite. The innermost sample rack, when sampling, has actually been disengaged from the rail 203 of the input base 200 and engaged with the grab plate 221. Thus, when sampling is completed, the innermost sample rack can be moved in the direction of the third screw 222 by the transfer screw-nut mechanism and moved to the sample bottle output mechanism 21 on one side, i.e., from the left to the right.

As shown in fig. 6, a scanning module 34 is disposed at the rear of the innermost sample bottle rack 201, specifically, the scanning module 34 is located at the rear of the rightmost sample bottle, the scanning module 34 performs identification scanning on the bar code attached to the sample bottle passing through the scanning module, and the scanning result is uploaded to a microcomputer for storage and processing, so as to correspond to the detection result of each final sample one by one. The scanning principle of the scanning module belongs to the prior art.

Further, as shown in fig. 5, the sample bottle positioning jack 2013 on the sample bottle rack 201 is provided with a positioning notch 2014 to cooperate with the positioning convex strip on the sample bottle, so as to define the angle when the sample bottle is put in, and ensure that the bar code position on the sample bottle faces to the inner side so as to be scanned by the scanning module 34.

The sample bottle output mechanism 21 includes an output base 210, the output base 210 being configured similarly to the input base. The inner side of the output base 210 is provided with an output driving shaft, two ends of the output driving shaft are respectively provided with an output thumb wheel (not shown in the figure), the output thumb wheel is positioned in the output base, and a plurality of thumb levers are arranged on one circle of the output thumb wheel. The structure of the output thumb wheel is the same as that of the input thumb wheel, but the positions are different, the thumb lever of the output thumb wheel can stir the sample rack outwards, namely forwards, and the sample rack enters the guide rail of the output base, and is similar to the entering of the sample rack, and the sample rack is also intermittently pushed outwards in sequence. The output base is provided with guide rails for sliding fit of the sample bottle rack on two sides, and the guide rail structure is arranged on the input base and is not repeated.

As shown in fig. 8, the tablet forming apparatus 4 includes a mold cup supply apparatus including a mold cup placement frame, a mold cup pushing mechanism 40, a mold cup discharging mechanism, and a mold cup transferring mechanism 42.

As shown in fig. 10, the mold cup placement frame includes a rectangular frame 43 in which two strings of mold cups a are placed, i.e., in a two-column distribution. The modeling of the mold cup is big in top and small in bottom, and can be just sleeved together up and down, and 10 mold cups are connected in series. The bottom-most mold cup, the cup edge at the upper part of the mold cup is supported by a support bar 431 in the frame. The mould cup is put into by corresponding operators, is used for dipping the sample liquid in the sample bin, and is further coated on a slide for tabletting. The mould cup belongs to disposable consumable, and after the mould cup is used, a new mould cup is put in.

According to the needs, in other embodiments, three strings of mold cups can be arranged, and one string can be set to 8; or 12 equal numbers.

As shown in fig. 10 and 11, a slide block 432 is provided at the bottom of the frame 43, the slide block 432 is driven by a screw nut mechanism, which is denoted as a die cup feed screw nut mechanism, and a screw is horizontally provided and denoted as a fourth screw 44, which is driven by a fourth servo motor 46. The sliding block 432 moves linearly along the fourth screw rod, and drives the frame 43 to move to the position of the mold cup pushing mechanism 40.

The die cup pushing mechanism 40 includes a screw-nut mechanism in which a nut is provided with a die cup pusher (not shown) that moves axially along a screw in the screw-nut mechanism, which is denoted as pushing screw 401. The movement direction of the die cup pushing plate is perpendicular to the movement direction of the frame 43, and the die cup pushing plate can push one of the strings of die cups into the die cup blanking mechanism integrally, so that the die cups are separated from the die cup placing frame. According to the requirement, the mould cup pushing plate can be temporarily and incompletely withdrawn, and the mould cup can stably enter the mould cup positioning frame. After the series of mold cups are used up, the frame continues to advance, and the mold cup pushing plate is used for pushing the other series of mold cups.

As shown in fig. 10 to 12, the mold cup blanking mechanism includes a mold cup positioning frame 45 and a material pulling mechanism 41, the mold cup positioning frame 45 is located at the opposite side of the mold cup pushing mechanism, the mold cup pushing plate moves to push a string of mold cups into the mold cup positioning frame 45, the mold cup positioning frame is a U-shaped opening, is just aligned to the mold cup pushing mechanism, and can limit the three directions of the string of mold cups, and of course, the mold cup positioning frame is matched with the function of the mold cup pushing plate, so that the stability of the stacked mold cups is ensured.

As shown in fig. 12, the material stirring mechanism 41 includes a fifth servo motor 411, the fifth servo motor coaxially drives a driving stirring shaft 413, a driving stirring wheel is arranged on the driving stirring shaft 413, a driven stirring shaft 414 parallel to the driving stirring shaft is arranged on the other side, and a driven stirring wheel 415 is arranged on the driven stirring shaft. The driving thumb wheel and the driven thumb wheel 415 are sector gears which are meshed with each other. The driving shifting shaft and the driven shifting shaft are respectively provided with a shifting block 412, and in the illustrated state, the two shifting blocks can just support the cup edge of the lowest mold cup A to prevent the falling of the mold cup A. When the blanking is needed, the fifth servo motor 411 acts, the two shifting blocks rotate at a certain angle along with the fifth servo motor, the limit is released, and the mold cup A can fall down by gravity. But soon, the fifth servo motor can reverse to reset the shifting block, and the cup edge of the lowest mold cup is supported and limited, namely only the next material is allowed at a time, and the discharged mold cup falls to the mold cup transferring mechanism. In other embodiments, the shifting block can also directly adopt a rectangular plate, and in a horizontal state, the cup edge of the mold cup can be supported, and after the set angle is turned over, the limit of the cup edge is relieved.

As shown in fig. 9, the mold cup transferring mechanism 42 includes a transferring seat 423, and two clamping holes 4231 are formed in the transferring seat 423, and each clamping hole can vertically place one mold cup. The transfer table is driven to perform linear motion by a die cup transfer screw nut mechanism, and a sixth screw 422 of the die cup transfer screw nut mechanism is driven by a sixth servo motor 421. The transfer seat 423 moves to the lower part of the material stirring mechanism 41, and after receiving a mold cup pulled down from the material stirring mechanism, the transfer seat continues to move a certain distance, and a second card Kong Qu is used for receiving a second mold cup. And after the two mold cups are connected, the transfer seat 423 moves reversely and leaves the lower position of the material stirring mechanism for the next link.

As shown in fig. 13, the slide making apparatus 4 further includes a smear mechanism 47, and the smear mechanism 47 includes a smear frame 471, which is driven by a corresponding screw-nut mechanism, and as shown in fig. 8, a screw in the screw-nut mechanism is denoted as a seventh screw 476, which is driven by a seventh servo motor. The smear frame 471 is axially movable in the left-right direction, that is, the seventh lead screw. The upper part of the smear frame is provided with a support arm which is provided with two mold cup rods 472 for inserting and taking out the two mold cups in the transfer seat 423. The mold cup rods 472 can be lifted, the lower ends of the mold cup rods are inserted into the mold cups and then lifted, the mold cup rods are driven by a seventh screw rod to move to the position of the sample bin 33, two sample bins are arranged, and the distance between the two sample bins is consistent with the distance between the two mold cup rods and corresponds to the distance between the two mold cup rods one by one. Each sample bin is provided with a sample liquid in a corresponding sample bottle, and can be dipped by a mould cup. The die cup rod descends to enable the lower portion of the die cup to be dipped with sample liquid in the sample bin. As further optimization, the mold cup rod is hollow, the upper part is connected with an air pipe, and can be pumped to form negative pressure to suck up the sample liquid; meanwhile, the bottom of the mold cup is provided with a plurality of filtering holes, and bacteria and the like to be detected can be adhered to the bottom of the mold cup.

Then, the mold cup lever is raised and moved to the slide placement stage 48 by the seventh screw.

As shown in fig. 13, the lifting mechanism of the cup lever 472 is as follows, the cup lever 472 is provided on the cup lever support 477 and driven by another screw-nut mechanism provided on the smear frame to realize the lifting movement of the cup lever in the up-down direction. The structure and principle of the screw-nut mechanism will not be described again, and the screw is shown in fig. 8 and is denoted as a mold cup lifting screw 470. The mold cup rod support seat 477 and the mold withdrawal plate 473 move up and down along the guide rod 479, and a spring 478 is connected between the mold cup rod support seat 477 and the mold withdrawal plate 473 to form a mold withdrawal device.

Two slide grooves are provided on the slide mount table 48, and two slides B are just mounted. The slide groove is two rectangular grooves, and the width of the slide groove is matched with the width of the slide. After the two mold cups are brought to the slide position by the mold cup rod, the mold cup rod is well in one-to-one correspondence, and the mold cup rod descends to coat the sample liquid dipped at the lower part of the mold cup on the slide, so that the slide preparation is completed.

To prevent the intersection of different sample liquids, it is necessary to clean the sample chamber 33 and the above-mentioned sampling pump and liquid tube with the set rinse liquid after the completion of the coating preparation of the two sample liquids. The sample bin is provided with a liquid injection hole for injecting quantitative flushing liquid, and after a certain time, the flushing liquid is sucked away again after the sampling hole is submerged in the sampling groove reaching the sampling seat 30 through the pump and the liquid pipe. The washing liquid after washing treatment can be arranged at the position of the sampling groove or the position of the sample bin, and the liquid can be sucked away by a pump, so that the washing liquid is easy to realize. After flushing, distilled water is used again to clean the flushing liquid. The injection and discharge of distilled water are similar to the flushing liquid, and do not involve complicated mechanism design, and thus will not be described in detail. For cleaning, the inner walls of the sample bin, the sampling seat and the like can be provided with non-adhesive layers.

As shown in fig. 13, after the smear is completed, the disposable mold cup needs to be removed, the mold cup rod is driven by the seventh screw rod to move to the position of the waste bin 475, the mold cup rod rises to the top and is mechanically collided, and at this time, the ejector plate 473 moves downward under the action of the spring 478, so that the mold cup is pushed down and falls into the waste bin 475.

After the smear mechanism completes one smear, i.e. after the mold cup is removed, the mold cup stem is also required to be rinsed and cleaned, similar to the rinsing of the sample bin and the sampling needle described above. The upper part of the mold cup rod is connected with a corresponding flushing pipeline, flushing liquid is pumped into the mold cup rod by a pump to flush the inner side; then distilled water is injected for flushing. The waste liquid can directly flow into a waste box and is cleaned periodically. The design of the valve for controlling on-off, the pump and the flushing pipeline is not the key point of the application, and belongs to the prior art.

The slide placement on the slide placement table 48 is accomplished by the cooperation of a slide pushing device and a robot device.

As shown in fig. 14 to 18, the slide pusher 5 includes a pusher frame 51, and a slide lifting screw nut mechanism is provided on one side of the pusher frame 51, and includes an eighth screw 521 and a slide lifting seat 522, and the eighth screw 521 is driven by an eighth servo motor 523. The slide lifting seat 522 can be lifted along the eighth screw rod to push the slide B of the stack in the slide placing frame 54 to lift, and intermittently lift for a set distance according to the setting, so as to achieve the purpose of pushing one slide at a time.

The slide placement frame 54 is a movable frame that facilitates manual placement into the pushing frame 51. The lower part of the slide placing frame is provided with a positioning protrusion 541 which is matched with a positioning groove arranged at the bayonet in the pushing frame to play a role in positioning. After the slide placement frame 54 is pushed horizontally into the bayonet 512 of the pushing frame, the slide placement frame 54 is supported by the bayonet positioning at two sides of the pushing frame, and is not pushed to move up and down, but only pushes a stack of slides therein to move up.

The slide placement frame 54 is open at the bottom and has a lip on the inside of the opening to support a stack of slides, the lip is not preferably wide, and the opening allows the slide lifting seat to contact the lowermost slide to push it upward. The side wall of the slide placing frame is also provided with a notch for the slide lifting seat to pass up and down.

The slide placement frame 54 has an open port in the upper portion to allow the slide to be lifted off the slide placement frame 54. After the slide is separated from the slide placing frame, the circumferential limit of the slide is limited by the limit wall of the pushing frame, the limit wall is provided with an inclined surface 513, a cavity with a small upper part and a large lower part is formed, the transition guiding function is achieved, the bottom of the cavity is slightly larger than the size of the slide, interference between the slide just coming out and the inner wall of the slide pushing frame is avoided, and smooth upward movement of the slide after the slide is separated from the slide placing frame is facilitated.

The uppermost slide is at the slide pushing mechanism waiting to be sent out.

As shown in fig. 17, the pusher mechanism includes a pusher screw nut mechanism, the screw of which is referred to as a ninth screw 532, and is driven by a ninth servomotor 531, and the ninth servomotor 531 is fixed to the upper portion of the pusher frame. A nut on the ninth lead screw 532, i.e., a pushing block 533, is used to push the slide. One end of the lower part of the pushing piece 533 is provided with a downward extending bulge, a pushing part 5331 for pushing one end of the slide is formed, and the bulge and the pushing piece matrix form a step surface of 90 degrees and are just matched with the end part of the slide. The length of the bulge is slightly smaller than the thickness of the slide, the exposed end part of the uppermost slide is just pushed, the rest slide is still positioned in the transition space, and the bulge can not be touched to push two slides at a time, and only one slide is pushed at a time.

The ninth lead screw 532 is spatially perpendicular to the eighth lead screw 521. The pushing block 533 moves linearly horizontally along the ninth screw 532, and the other end of the slide is pushed by the pushing block to protrude from the slide outlet 511 of the pushing frame, but is not completely pushed out, and the protruding portion is required to wait for the gripping by the robot device 6.

As shown in fig. 19 and 20, the robot apparatus 6 includes an X-axis mechanism 61, a Y-axis mechanism 63, a Z-axis mechanism 62, and a rotation mechanism 64, and the rotation mechanism 64 is provided with a holding jaw 641 for holding a slide extending from the pushing frame and transferring the slide to the slide placing table 48 for smear. After the slide is produced, the slide is transferred to the dyeing device 7 by the manipulator device for dyeing operation, or the slide can be transferred to the slide storage device for temporary storage under the condition that the dyeing device is in operation.

The manipulator device mainly uses the functions achieved by the manipulator device, the specific structure is not the focus of the application, and prior patents (the same applicant as the application) have disclosed similar functions and principles, such as a manipulator device capable of freely grabbing a slide, disclosed in the patent application publication No. CN 110587585A. Hereinafter, only the manipulator device will be described roughly:

the X-axis mechanism 61 includes an X-axis screw nut mechanism, the Z-axis mechanism 62 includes a Z-axis screw nut mechanism and a Z-axis moving frame 621, and the Y-axis mechanism 63 includes a Y-axis screw nut mechanism and a Y-axis moving frame 631. The X-axis nut of the X-axis screw nut mechanism drives the Z-axis moving frame to linearly move in the left-right direction, that is, axially move along the X-axis screw 611. The Z-axis nut of the Z-axis screw nut mechanism drives the Y-axis moving rack 631 to move up and down, that is, along the Z-axis screw 622. The Y-axis nut of the Y-axis screw nut mechanism drives the rotation mechanism 64 to move in the front-rear direction, that is, on the Y-axis screw 632 of the Y-axis moving frame.

The rotation mechanism 64 includes a fixed frame, a rotation arm 642, and a rotation motor driving the rotation arm, the fixed frame being fixed to the Y-axis moving frame 631, the rotation motor being fixed to the fixed frame. The direction of the rotation axis of the rotation arm is spatially perpendicular to the Y-axis screw. The end of the rotating arm is provided with a clamping jaw 641, and the opening and closing of the clamping jaw is driven by a clamping jaw motor or a cylinder if the clamping jaw is a pneumatic clamping jaw, which is the existing mature technology. The rotary motor may be a stepper motor, and in this embodiment the rotary arm is movable through a range of substantially 90 degrees, such as to control the jaw to switch between a horizontal position and a downward vertical position.

Each screw rod in the manipulator device is driven by a corresponding servo motor or stepping motor.

The operational characteristics of the manipulator device are briefly described below. The clamping jaw is in a horizontal position, under the cooperation of the X, Y, Z shaft screw nut mechanism, the clamping jaw grabs a slide extending out of the pushing frame 51, then withdraws along the Y axis, moves along the X axis, descends along the Z axis, places the slide in a slide groove of the slide placing table 48, and after the slide is placed in place, loosens and returns to grab the slide again.

After the slide is smeared, the slide is transferred away by a manipulator device, and is moved to a dyeing device under the cooperation of a X, Y, Z shaft screw nut mechanism, and then the clamping jaw is turned over by 90 degrees through the adjustment of a rotating mechanism, so that the slide is vertical and is inserted into a dyeing bin in the dyeing device 7.

In the present embodiment, as shown in fig. 21 to 23, the staining apparatus is provided with four rows of 24 staining bins 71, and 24 slides B can be inserted and stained simultaneously. The structure and principle of the dyeing device are as disclosed in the application patent of publication No. CN110286023A, and the dyeing structure body comprises 12 dyeing bin bodies in total. Therefore, only the dyeing apparatus in this embodiment will be briefly described below.

Each dyeing bin body 71 of the dyeing device is provided with a dyeing cavity with an upper opening, the lower part of the dyeing cavity is provided with a liquid inlet and outlet hole 72, the upper part of the dyeing cavity is provided with an anti-overflow hole 73, and excessive dyeing liquid can be discharged through the anti-overflow hole 73. The dye liquid is injected and discharged through the liquid inlet/outlet holes 72. A plurality of dyeing liquid bottles are arranged in a shell of the full-automatic dyeing scanning system, dyeing liquid required by dyeing is provided, and the types of the dyeing liquid are selected according to the needs. The dyeing cavity is used for vertically inserting the slide, and the slide is inserted by the manipulator device. The stained slide is also clamped out by the manipulator device and transferred to a scanning device for scanning detection.

As shown in fig. 24 to 26, the scanning device 8 includes a scanning microscope 81 and a slide automatic alignment device 82, and the slide automatic alignment device 82 is provided on one side of the staining device 7 and is located close to the scanning microscope. The automatic slide aligning device comprises an up-down aligning mechanism 821 and a left-right aligning mechanism, wherein the up-down aligning mechanism 821 comprises a fixed bracket 8211 and an up-down screw nut mechanism, a screw in the up-down screw nut mechanism is named as a tenth screw 8213, the tenth screw 8213 is vertically arranged, the tenth screw is driven by a tenth servo motor 8212, a nut on the tenth screw, namely an up-down moving seat 8214, is guided and assembled on a sliding rail on the fixed bracket 8211, and can move up and down.

The left-right alignment mechanism includes a left-right screw nut mechanism 822 and a glass mount 83 for placing a stained slide, and the left-right screw nut mechanism 822 is provided in the up-down movement base 8214. The screw in the left and right screw nut mechanisms is referred to as an eleventh screw 8223, and the driving motor associated with the screw is referred to as an eleventh servo motor 8221. The eleventh screw 8223 is axially disposed in the left-right direction, and two ends thereof are rotatably mounted on corresponding support bases 8222, and the support bases 8222 are fixed to the up-down movement base 8214.

The nut in the left and right screw nut mechanism, that is, the slide seat 8224, is driven by the eleventh screw 8223, and the slide seat 8224 moves in the left and right direction. The glass mount 83 is fixed to the slide base 8224. Two slide positioning grooves 831 are arranged on the glass carrier 83, positioning step surfaces 8312 are arranged at four corners of the slide positioning grooves, and four corners of the slide B are positioned. The slide can be placed in the up-down direction under the grip of the jaws of the robot device. The adjacent slide positioning grooves are communicated.

As shown in fig. 26, a through hole 8311 is formed at the bottom of the slide positioning groove to form a light hole corresponding to the sample position of the slide, so that the scanning by a microscope is facilitated. The front side of slide positioning groove is equipped with the mouth 8313 of stepping down, and the mouth 8313 of stepping down is located the below of slide B tip so that the clamping jaw can pass through this mouth 8313 of stepping down, and the slide can be placed in place.

As shown in fig. 26, two slide buffer tanks 832 are further provided on the glass stage 83 for temporarily storing the stained slides to wait for scanning. The slide buffer groove is a rectangular groove with one end open, and the length of the slide buffer groove is smaller than that of the slide positioning groove, so that the end part of the slide can extend out of the open end for clamping by the clamping jaw.

The upper and lower alignment mechanism can adjust the height position of the slide. The left and right alignment mechanism can adjust the left and right positions of the slide. The slide is sent to a proper position of a scanning microscope which can be scanned, and after one slide is scanned and detected, the glass carrier table continues to move horizontally so as to scan the other slide. In the scanning process, when the height position of the slide needs to be adjusted, the slide height position can also be adjusted by the up-down positioning mechanism. Scanning microscope, using the prior art. After the scanning is finished, the glass carrier is reset, and a new glass slide is loaded again. The scanning microscope is connected with the microcomputer, can directly process the scanning result and display the scanning result on the display screen, and can also be directly connected with the printer to print the result. The scanned slide is clamped by the manipulator device and then placed into the slide storage device 9 for subsequent centralized processing, or a collecting box of the scanned slide can be specially arranged.

The slide storage device can be seen from the patent of application publication No. CN110589320A (same as the applicant), and has the same structure and principle, and is only briefly described here.

As shown in fig. 27, the slide storage device includes a slide magazine bracket, on which a plurality of slide cassette insertion openings 92 are provided, and each slide cassette insertion opening 92 is correspondingly assembled with a slide cassette 91 in a drawing manner. The slide box comprises a box body, wherein the box body is provided with a plurality of slide slots, and each slide slot is provided with a slot cavity for inserting a slide. In this embodiment, a box body is provided with two rows of 24 slide slots, and a group of 24 slides just corresponds to the staining device. For ease of use, the cassette is provided with at least two.

In this embodiment, the slide box is retractable, and in some special cases, the slide box can be manually operated, for example, manual slides of some emergency patients can be manually put into the slide box and pushed into the slide bin bracket, and the batch of slides can be preferentially scanned by adjusting the program of the system. The slide storage device can be placed in a slide box mode for only preparing and undyed slides, then the system program is changed, the manipulator device transfers the slides to the dyeing device one by one, the slides are inserted into a dyeing bin body, and after dyeing is finished, the slides are taken out by the manipulator device and transferred to the scanning device for scanning.

The full-automatic film-making dyeing scanning system has the functions of automatic sampling, automatic smear, automatic dyeing, automatic film reading and the like, is suitable for sputum, bronchial flushing liquid, hydrothorax, ascites, urine, needle aspiration puncture liquid, cerebrospinal fluid and other tuberculosis samples, meets the diagnosis requirements of all stages of tuberculosis laboratories on the tuberculosis smear, and can also be used by the professionals of conventional laboratories and research institutions.

In other embodiments, the mold cup blanking mechanism may be designed as follows:

the mould cup locating frame is provided with two, namely two U-shaped openings which are arranged side by side. Two strings of mold cups placed in the mold cup placement frame can be pushed into the mold cup positioning frame at one time, and one string of mold cups corresponds to one mold cup positioning frame. Meanwhile, two stirring mechanisms are arranged, and one stirring mechanism can control one die cup to drop at a time for blanking, but the total of the two die cups can be used for blanking at a time, and the two die cups just correspond to the two clamping holes on the die cup transfer seat.

In the above preferred embodiment, rotation of the dial is used to control dropping of the cup. In other embodiments, the kick-out mechanism may be designed as follows:

the poking rod capable of linearly stretching is used for replacing the poking shaft and the poking block, the poking rod can be driven by a servo electric push rod or an air cylinder, the poking rod stretches out to support the cup edge of the lowest mould cup, when the mould cup is required to fall down, the poking rod retracts to release the limit, and after the mould cup falls down, the poking rod is quickly reset to support the cup edge of the lowest mould cup. The deflector rod can adopt a rectangular rod or a structure with edges at the supporting part, and has better supportability than a round rod.

Of course, in other embodiments, the lifting of the die plate can be driven by an air cylinder, and the purpose of pushing down the die cup can also be achieved. Or the die plate is not required to be arranged, a baffle is arranged on one side of the waste box, when the die cup to be withdrawn moves to the position, the die cup edge is positioned below the baffle, and when the die cup rod drives the die cup to rise, the die cup is naturally limited by the baffle and cannot rise to fall.

Claims

1. An automatic film-making device, its characterized in that: the device comprises a mold cup feeding device and a smear mechanism, wherein the mold cup feeding device comprises a mold cup placing frame, a mold cup pushing mechanism, a mold cup discharging mechanism and a mold cup transferring mechanism; the mould cup placing frame comprises a frame which can move linearly and is used for placing the mould cups in a pile, and the frame sends the mould cups to the mould cup pushing mechanism;

the die cup pushing mechanism comprises a die cup pushing plate which moves linearly to push the piled die cups into the die cup blanking mechanism; the die cup blanking mechanism comprises a die cup positioning frame and a stirring mechanism, wherein the die cup positioning frame is positioned above the stirring mechanism and used for positioning a pushed die cup, the stirring mechanism comprises a stirring block capable of intermittently rotating or a stirring rod capable of intermittently stretching, the stirring block or the stirring rod supports the lowest die cup, and one die cup can drop at a time in the process of rotating or stretching the stirring rod;

the mold cup transferring mechanism comprises a transferring seat, a clamping hole for placing the mold cup is arranged on the transferring seat, and the transferring seat transfers the mold cup away from the material stirring mechanism;

the smear mechanism comprises a smear frame capable of horizontally moving, a liftable mold cup rod is arranged at the upper part of the smear frame and used for inserting and taking a mold cup in the transfer seat, and the mold cup dipped with the sample liquid is sent to a slide placing table to smear a slide on the slide placing table; the stirring mechanism comprises a driving stirring shaft and a driven stirring shaft, the axes of the driving stirring shaft and the driven stirring shaft are parallel, a driving stirring wheel and a driven stirring wheel are respectively arranged on the driving stirring shaft and the driven stirring shaft, the driving stirring wheel and the driven stirring wheel are in meshed transmission, one stirring block is respectively arranged at the end parts of the driving stirring shaft and the driven stirring shaft, the two stirring blocks are oppositely arranged, the stirring block supports the lowest mold cup, the stirring blocks can intermittently rotate, and one mold cup can drop at a time in the rotating process of the stirring block.

2. An automated film making apparatus according to claim 1, wherein: more than two rows of mold cups are arranged in the frame, the mold cups in each row are sleeved together up and down, the upper part of each mold cup is provided with a cup edge, and a support bar is arranged at the bottom in the frame and supports the cup edge of the lowest mold cup.

3. An automated film making apparatus according to claim 2, wherein: the bottom of the frame is provided with a sliding block which is driven by a screw nut mechanism.

4. An automated film making apparatus according to claim 1, wherein: the die cup positioning frame is a U-shaped frame, and an opening faces one side of the die cup pushing plate; or the mold cup positioning frame is provided with more than two U-shaped openings so as to correspond to each stack of mold cups.

5. An automated film making apparatus according to claim 1, wherein: the driving shifting wheel and the driven shifting wheel are sector gears.

6. An automated film making apparatus according to claim 1, wherein: the smear frame is driven by a corresponding screw nut mechanism, a supporting arm is arranged at the upper part of the smear frame, and a mold cup rod is arranged on the supporting arm and can move up and down; the smear frame is provided with a die withdrawing device, the die withdrawing device comprises a die withdrawing plate arranged on a guide rod, a die cup rod supporting seat for supporting a die cup rod is arranged on the upper portion of the die withdrawing plate, the die cup rod supporting seat is positioned above the die withdrawing plate, the die cup rod supporting seat is assembled on the guide rod in a guiding way, and a spring is arranged between the die withdrawing plate and the die cup rod supporting seat.

7. An automated film making apparatus according to claim 6, wherein: and a waste box is further arranged on one side of the smear frame and used for collecting used mold cups.

8. An automated film making apparatus according to claim 1, wherein: and one side of the transfer seat is also provided with a sample bin for temporarily storing sample liquid for dipping by the mold cup.