CN111548913A

CN111548913A - Preparation method and application of kitchen waste microbial strain

Info

Publication number: CN111548913A
Application number: CN202010412876.2A
Authority: CN
Inventors: 金振兴; 卞义伦
Original assignee: Suzhou Zhongjieyu Technology Co ltd
Current assignee: Suzhou Zhongjieyu Technology Co ltd
Priority date: 2020-05-15
Filing date: 2020-05-15
Publication date: 2020-08-18

Abstract

The invention discloses a manufacturing method of kitchen waste microbial strains and application thereof, belonging to the field of microbial strains, and being capable of realizing rapid manufacturing of probiotic strains, reducing manufacturing difficulty by simplifying manufacturing flow, so that a large-scale farm can manufacture microbial strains by self, the purchase cost is saved, the adopted manufacturing raw materials are low in cost and easy to purchase, in addition, a fermentation barrel for manufacturing the strains is internally provided with a stirring device, the infection of foreign bacteria can be avoided, in addition, two baffles are arranged in a ventilation hole for ventilation on a barrel cover, the two baffles are provided with ventilation holes, the upper ventilation hole and the lower ventilation hole are arranged in a staggered mode, so that the kitchen waste microbial strains are breathable and light-proof, and the dark condition required by strain generation is met.

Description

Preparation method and application of kitchen waste microbial strain

Technical Field

The invention relates to the field of microbial strains, in particular to a preparation method and application of a microbial strain of kitchen waste.

Background

The microbial bacteria seed-feed fermentation strain and the biological fertilizer fermentation strain can compensate amino acid which is easy to lack in conventional feed, can quickly convert nutrient components of other coarse feed raw materials, and achieve the effect of enhancing digestion, absorption and utilization.

Therefore, probiotic strains are often added into the feed in the breeding industry for feeding, the quality of meat can be improved, the production of the probiotic strains is mostly in an industrial mode at present, the production process is complex, the consumed time is long, the cost of raw materials for production is high, and the produced economic benefit is low.

Disclosure of Invention

1. Technical problem to be solved

Aiming at the problems in the prior art, the invention aims to provide a method for manufacturing kitchen waste microorganism strains and application thereof, which can realize the quick manufacturing of probiotic strains, reduce the manufacturing difficulty by simplifying the manufacturing process, so that a large-scale farm can manufacture the microorganism strains by self, save the purchase cost, have low cost of adopted manufacturing raw materials and are easy to purchase, in addition, a fermentation barrel for manufacturing the strains is internally provided with a stirring device, the infection of foreign bacteria can be avoided, two baffles are arranged in ventilation holes for ventilation on a barrel cover, ventilation holes are arranged on the two baffles, the upper ventilation holes and the lower ventilation holes are arranged in a staggered manner, so that the kitchen waste microorganism strains are breathable and light-proof, and the dark condition required by strain generation is met.

2. Technical scheme

In order to solve the above problems, the present invention adopts the following technical solutions.

A manufacturing method of kitchen waste microbial strains comprises a fermentation barrel, a barrel cover matched with the fermentation barrel, 2 parts of probiotic strains, 4 parts of cane sugar, 1 part of glucose and 90 parts of raw water, and comprises the following steps:

s1, pouring 4 parts of cane sugar into a fermentation barrel;

s2, pouring 90 parts of raw water into the fermentation barrel, and stirring;

s3, pouring 2 parts of probiotic strains into the fermentation barrel, uniformly stirring, and then covering a barrel cover;

s4, when the fermentation days are 10-12 days, adding 1 part of glucose, and after 2-3 days, purifying and filtering for extraction.

Furthermore, the temperature in the fermentation barrel is kept between 25 ℃ and 35 ℃, and microorganisms can die due to overhigh or overlow temperature and can well grow at room temperature.

Further, after 2 to 3 days after the operation of the step S3, 1 part of a fermentation accelerator is added to the fermenter, and the fermentation accelerator can accelerate the fermentation process of the microbial strains.

Furthermore, a discharge pipe is communicated with the side wall of one side of the lower end of the fermentation barrel, a vertically arranged rotating rod is rotatably connected to the middle of the fermentation barrel, a plurality of stirring blades which are arranged in a surrounding manner are fixedly connected to the rotating rod, a rotating handle is fixedly connected to the upper end of the rotating rod, a feeding pipe is communicated to the barrel cover, a cover plate is rotatably connected to an opening at the upper end of the feeding pipe, a ventilation hole is formed in one side of the barrel cover, which is away from the feeding pipe, two transversely arranged baffle plates are fixedly connected to the inner wall of the ventilation hole, a plurality of ventilation holes are formed in the two baffle plates, the upper ventilation hole and the lower ventilation hole are arranged in a staggered manner, a stirring device is arranged in the fermentation barrel, infection of foreign bacteria can be avoided, two baffle plates are arranged in the ventilation hole for ventilation in the barrel cover, ventilation holes are formed in the two, the dark black condition required by strain generation is met.

Further, a plurality of supports of inner wall fixedly connected with of fermenter, and fixedly connected with rolling bearing between a plurality of supports, the bull stick runs through rolling bearing, and bull stick and rolling bearing's inner circle fixed connection, and rolling bearing plays stable effect to the rotation of bull stick to avoid the bull stick slope.

Further, the upper end fixedly connected with handle of bung, and the parcel has the foam-rubber cushion on hand, the bung of conveniently taking of handle, the comfort level of foam-rubber cushion increase hand.

Furthermore, the side wall of the free end of the cover plate is provided with a groove, and the groove is convenient for fingers to lift the cover plate.

Furthermore, the inner wall of the bottom of the fermentation barrel is conical, the lower end of the stirring blade is parallel to the inner wall of the bottom of the fermentation barrel, the conical bottom is convenient for discharging, and the lower end of the stirring blade is parallel to the bottom of the fermentation barrel so as to stir liquid at the bottom.

Further, every the stirring leaf is formed by the body of rod and the vertical body of rod welding of a plurality of slopes, and the stirring leaf can cover the inside of whole fermenter, and stirring range is big, guarantees the stirring.

A preparation method and application of kitchen waste microbial strains comprise the following steps:

s1, fermenting feed raw materials and a formula: 200 parts of prepared fermented feed, 70-90 parts of water, 1 part of probiotic strain and 150 g of bacillus natto;

s2, preparing diluted activated fermentation liquor: pouring probiotic strains and bacillus natto into water, and uniformly stirring to prepare activated fermentation liquor;

s3, mixing the prepared activated fermentation broth and the fermented feed uniformly, kneading the activated fermentation broth into a ball by hand with humidity without dripping water, and dispersing the ball by touch;

s4, compacting and stacking the prepared feed on the ground, and sealing with a plastic film.

3. Advantageous effects

Compared with the prior art, the invention has the advantages that:

(1) this scheme can realize the quick preparation of probiotic bacterial, reduce the degree of difficulty of preparation through simplifying the preparation flow, large-scale plant can oneself make the microbial bacterial species like this, the acquisition cost has been practiced thrift, and the preparation raw materials cost who adopts is also low, also easy purchase, be used for making in the fermentation vat of bacterial in addition from taking agitating unit, can avoid the infection of external miscellaneous fungus, and be provided with two baffles in the scavenge port that is used for taking a breath on the bung, all set up the air vent on two baffles, two upper and lower air vents crisscross setting, can breathe freely and light-tight like this, satisfy the required dark condition of bacterial generation.

(2) The temperature in the fermentation barrel is kept between 25 ℃ and 35 ℃, and microorganisms can die when the temperature is too high or too low, so that the microorganisms can well grow at room temperature.

(3) And (S3) after 2-3 days, adding 1 part of fermentation accelerator into the fermentation vat, wherein the fermentation accelerator can accelerate the fermentation process of the microbial strains.

(4) The side wall of one side of the lower end of the fermentation barrel is communicated with a discharge pipe, the middle part of the fermentation barrel is rotationally connected with a vertically arranged rotating rod, a plurality of stirring blades which are arranged around the rotating rod are fixedly connected on the rotating rod, a rotating handle is fixedly connected at the upper end of the rotating rod, a feeding pipe is communicated on the barrel cover, and the opening at the upper end of the feeding pipe is rotatably connected with a cover plate, one side of the barrel cover far away from the feeding pipe is provided with a vent hole, and the inner wall of the ventilation hole is fixedly connected with two transversely arranged baffles, a plurality of ventilation holes are arranged on the two baffles, the upper and lower air holes are arranged in a staggered way, and the fermentation barrel is internally provided with a stirring device, so that the infection of foreign bacteria can be avoided, and two baffles are arranged in the ventilation holes for ventilation on the barrel cover, the two baffles are provided with ventilation holes, and the upper ventilation hole and the lower ventilation hole are arranged in a staggered manner, so that the ventilation and the light-proof performance are realized, and the dark and dark conditions required by strain generation are met.

(5) The inner wall fixedly connected with a plurality of supports of fermenter, and fixedly connected with rolling bearing between a plurality of supports, the bull stick runs through rolling bearing, and bull stick and rolling bearing's inner circle fixed connection, and rolling bearing plays stable effect to the rotation of bull stick to avoid the bull stick slope.

(6) The upper end fixedly connected with handle of bung, and the parcel has the foam-rubber cushion on hand, and the bung conveniently takes to the handle, and the comfort level of foam-rubber cushion increase hand.

(7) The free end side wall of the cover plate is provided with a groove which is convenient for fingers to lift the cover plate.

(8) The bottom inner wall of the fermentation barrel is conical, the lower end of the stirring blade is parallel to the bottom inner wall of the fermentation barrel, the conical bottom is convenient for discharging, and the lower end of the stirring blade is parallel to the bottom of the fermentation barrel so as to stir the liquid at the bottom.

(9) Every stirring leaf forms by the body of rod and the vertical body of rod welding of a plurality of slopes, and the stirring leaf can cover the inside of whole fermenter, and stirring range is big, guarantees the stirring.

Drawings

FIG. 1 is a flow chart illustrating the fabrication of the present invention;

FIG. 2 is a schematic front view of the present invention;

FIG. 3 is a schematic cross-sectional view of a barrel lid according to the present invention;

FIG. 4 is a schematic cross-sectional view of a fermenter according to the present invention;

FIG. 5 is a schematic view of the structure at A in FIG. 3;

fig. 6 is a schematic structural diagram at B in fig. 3.

The reference numbers in the figures illustrate:

1 fermenter, 2 bung, 3 discharging pipes, 4 bull sticks, 5 stirring leaves, 6 change handle, 7 feeding pipes, 8 apron, 9 ventholes, 10 baffle, 11 air vents, 12 supports, 13 rolling bearing, 14 handles, 15 digging groove.

Detailed Description

The drawings in the embodiments of the invention will be combined; the technical scheme in the embodiment of the invention is clearly and completely described; obviously; the described embodiments are only some of the embodiments of the invention; but not all embodiments, are based on the embodiments of the invention; all other embodiments obtained by a person skilled in the art without making any inventive step; all fall within the scope of protection of the present invention.

In the description of the present invention, it should be noted that the terms "upper", "lower", "inner", "outer", "top/bottom", and the like indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the referred device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and thus should not be construed as limiting the present invention. Furthermore, the terms "first" and "second" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "disposed," "sleeved/connected," "connected," and the like are to be construed broadly, e.g., "connected," which may be fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.

Example 1:

referring to fig. 1-6, a method for preparing a kitchen waste microbial strain, referring to fig. 1, comprises a fermentation vat 1 and a vat cover 2 matched with the fermentation vat, 2 parts of probiotic strains, 4 parts of sucrose, 1 part of glucose and 90 parts of raw water, and comprises the following steps:

s1, pouring 4 parts of sucrose into the fermentation barrel 1, keeping the temperature in the fermentation barrel 1 between 25 and 35 ℃, leading to microorganism death when the temperature is too high or too low, and leading to good growth at room temperature;

s2, pouring 90 parts of raw water into the fermentation barrel 1, and stirring;

s3, pouring 2 parts of probiotic strains into the fermentation barrel 1, uniformly stirring, covering the barrel cover 2, and adding 1 part of fermentation accelerant into the fermentation barrel 1 after 2-3 days after the operation of the step S3 is finished, wherein the fermentation accelerant can accelerate the fermentation process of the microbial strains;

s4, adding 1 part of glucose when the fermentation days are 10-12 days, and purifying and filtering for extraction after 2-3 days;

referring to fig. 2-6, the fermentation barrel 1 and the barrel cover 2 are in threaded connection, the upper end of the barrel cover 2 is fixedly connected with a handle 14, the handle 14 is wrapped with a sponge pad, the handle 14 facilitates taking the barrel cover 2, the sponge pad increases the comfort of the hand, the side wall of one side of the lower end of the fermentation barrel 1 is communicated with a discharge pipe 3, the middle part of the fermentation barrel 1 is rotatably connected with a vertically arranged rotating rod 4, the inner wall of the fermentation barrel 1 is fixedly connected with a plurality of brackets 12, a rotating bearing 13 is fixedly connected between the brackets 12, the rotating rod 4 penetrates through the rotating bearing 13, the rotating rod 4 is fixedly connected with the inner ring of the rotating bearing 13, the rotating bearing 13 plays a stabilizing role in rotating the rotating rod 4 so as to prevent the rotating rod 4 from inclining, the rotating rod 4 is fixedly connected with a plurality of stirring blades 5 which are arranged around, the inner wall of the bottom of the fermentation barrel 1 is in a conical shape, and the lower end of the stirring blades 5 is parallel, the conical bottom is convenient for discharging, the lower ends of the stirring blades 5 are parallel to the bottom of the fermentation barrel 1 so as to stir liquid at the bottom, each stirring blade 5 is formed by welding a plurality of inclined rod bodies and a vertical rod body, the stirring blades 5 can cover the inside of the whole fermentation barrel 1, the stirring range is large, uniform stirring is guaranteed, the upper end of the rotating rod 4 is fixedly connected with a rotating handle 6, a feeding pipe 7 is communicated with the barrel cover 2, a cover plate 8 is rotatably connected to an opening at the upper end of the feeding pipe 7, a free end side wall of the cover plate 8 is provided with a groove digging 15, the groove digging 15 facilitates fingers to lift the cover plate 8, one side of the barrel cover 2, far away from the feeding pipe 7, is provided with a ventilation hole 9, the inner wall of the ventilation hole 9 is fixedly connected with two transversely arranged baffles 10, the two baffles 10 are both provided with a plurality of ventilation;

The fermentation tank and the tank cover are sterilized before the fermentation tank is manufactured, 4 parts of cane sugar are poured into the fermentation tank 1, 90 parts of raw water is poured into the fermentation tank 1, the rotating rod 4 is rotated to drive the stirring blades 5 to stir, the bottom of each stirring blade 5 is parallel to the bottom of the fermentation tank 1, so that liquid at the bottom can be stirred, 2 parts of probiotic strains are poured into the fermentation tank 1, the tank cover 2 is covered after the probiotic strains are uniformly stirred, 1 part of glucose is added when the fermentation days are 10-12 days, the microbial strains flow out of the discharge pipe 3 after 2-3 days, the probiotic strains can be extracted and used after purification and filtration, in the fermentation process, the strains do not need to be completely sealed, dark conditions are needed, and therefore the upper vent hole 11 and the lower vent hole 11 are arranged in a staggered mode, and therefore the air permeable and light-proof effects are achieved.

Can realize the quick preparation of probiotic bacterial, reduce the degree of difficulty of preparation through simplifying the preparation flow, large-scale plant can oneself make the microbial bacterial like this, the acquisition cost has been practiced thrift, and the preparation raw materials cost who adopts is also low, also easy purchase, be used for making in the fermenter of bacterial in addition from taking agitating unit, can avoid the infection of external miscellaneous fungus, and be provided with two baffles in the scavenge port that is used for taking a breath on the bung, all set up the air vent on two baffles, two upper and lower air vents crisscross setting, can breathe freely and light-tight like this, satisfy the required dark condition of bacterial generation.

The above; but are merely preferred embodiments of the invention; the scope of the invention is not limited thereto; any person skilled in the art is within the technical scope of the present disclosure; the technical scheme and the improved concept of the invention are equally replaced or changed; are intended to be covered by the scope of the present invention.

Claims

1. The manufacturing method of the kitchen waste microbial strains comprises a fermentation barrel (1), a barrel cover (2) matched with the fermentation barrel, 2 parts of probiotic strains, 4 parts of cane sugar, 1 part of glucose and 90 parts of raw water, and is characterized in that: the method comprises the following steps:

s1, pouring 4 parts of cane sugar into the fermentation barrel (1);

s2, pouring 90 parts of raw water into the fermentation barrel (1) and stirring;

s3, pouring 2 parts of probiotic strains into the fermentation barrel (1), uniformly stirring, and then covering the barrel cover (2);

2. The method for preparing microbial strains of kitchen waste according to claim 1, wherein the method comprises the following steps: the temperature in the fermentation barrel (1) is kept between 25 and 35 ℃.

3. The method for preparing microbial strains of kitchen waste according to claim 1, wherein the method comprises the following steps: and (3) after the operation of the step S3 is finished, adding 1 part of fermentation accelerator into the fermentation vat (1) after 2-3 days.

4. The method for preparing microbial strains of kitchen waste according to claim 1, wherein the method comprises the following steps: the fermentation barrel is characterized in that a discharging pipe (3) is communicated with the side wall of one side of the lower end of the fermentation barrel (1), a rotating rod (4) which is vertically arranged is connected to the middle of the fermentation barrel (1) in a rotating mode, a plurality of stirring blades (5) which are arranged in a surrounding mode are fixedly connected to the rotating rod (4), a rotating handle (6) is fixedly connected to the upper end of the rotating rod (4), a feeding pipe (7) is communicated to the barrel cover (2), a cover plate (8) is connected to the opening of the upper end of the feeding pipe (7) in a rotating mode, ventilation holes (9) are formed in one side, away from the feeding pipe (7), of the barrel cover (2), two transverse baffles (10) of the ventilation holes (9) are fixedly connected to the inner wall of the baffles (10), a plurality of ventilation holes (11) are formed.

5. The manufacturing method of kitchen waste microbial strains according to claim 4, characterized by comprising the following steps: the inner wall fixedly connected with of fermenter (1) has a plurality of supports (12), and fixedly connected with rolling bearing (13) between a plurality of supports (12), rolling bearing (13) is run through in bull stick (4), and the inner circle fixed connection of bull stick (4) and rolling bearing (13).

6. The manufacturing method of kitchen waste microbial strains according to claim 4, characterized by comprising the following steps: the upper end of the barrel cover (2) is fixedly connected with a handle (14), and the handle (14) is wrapped with a spongy cushion.

7. The manufacturing method of kitchen waste microbial strains according to claim 4, characterized by comprising the following steps: the free end side wall of the cover plate (8) is provided with a digging groove (15).

8. The method for preparing microbial strains of kitchen waste according to claim 1, wherein the method comprises the following steps: the bottom inner wall of the fermentation barrel (1) is conical, and the lower end of the stirring blade (5) is parallel to the bottom inner wall of the fermentation barrel (1).

9. The method for preparing microbial strains of kitchen waste according to claim 1, wherein the method comprises the following steps: each stirring blade (5) is formed by welding a plurality of inclined rod bodies and a vertical rod body.

10. The preparation method and the application of the microbial strains of the kitchen waste according to any one of claims 1 to 9, wherein the preparation method comprises the following steps: the method comprises the following steps: