CN111527989B - Culture medium of Firmiana hirsuta, artificial cultivation method and application - Google Patents
Culture medium of Firmiana hirsuta, artificial cultivation method and application Download PDFInfo
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- CN111527989B CN111527989B CN202010249784.7A CN202010249784A CN111527989B CN 111527989 B CN111527989 B CN 111527989B CN 202010249784 A CN202010249784 A CN 202010249784A CN 111527989 B CN111527989 B CN 111527989B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
Abstract
The invention provides a culture medium of Firmiana simplex, an artificial cultivation method and application, wherein the culture medium comprises a liquid culture medium, a cultivation culture medium and a preservation culture medium, and the artificial cultivation method comprises the following steps: inoculating a crude phellinus igniarius preservation strain to a solid culture medium to obtain an activated strain, and inoculating the activated strain to a liquid culture medium to culture to obtain a liquid strain; preparing a culture medium; diluting the liquid strain, inoculating to culture medium, culturing by light culture and oxygen-enriched culture until the Inonotus hirsutus is mature, and harvesting. The invention has the advantages that: the culture medium takes mulberry leaf and mulberry twig mixture and sawdust as main raw materials, the production period of the crude capillary fungus is shortened and the yield is improved by adjusting the formula of the culture medium and optimizing the environmental conditions, and the culture conditions suitable for artificially culturing the crude capillary fungus are found by light culture and oxygen-enriched culture, so that the hyphae grow vigorously, the primordium is differentiated quickly, and the culture time is short.
Description
Technical Field
The invention belongs to the technical field of edible fungi, relates to cultivation of edible fungi, and particularly relates to a culture medium of Ficus hirsuta, an artificial cultivation method and application.
Background
The crude phellinus hirsutus (Inonotus hispidus) is named as Xanthochrous fuscus hispidus (Xanthochrous hispidus) and belongs to Basidiomycota (Basidiomycota), Agaricaceae (Agaric fungi), Hymenochaetales (Hymenochaetales), Hymenochaetaceae (Hymenochaetaceae) and Microchaetaceae (Inonotus), and has important nutritional value and medicinal value.
Firmiana hirsuta is a wood-rotting medicinal fungus growing in temperate zones, mainly growing on standing woods and occasionally growing on fallen woods. Fruiting bodies appear in summer and autumn, turn black after late autumn, and are mainly parasitic on broad-leaved trees such as fraxinus mandshurica, elm, mulberry, apple tree and Japanese pagodatree, the fruiting bodies are mainly parasitic on fraxinus mandshurica in northeast and are common on mulberry trees in northwest.
The crude phellinus linteus as a medicinal fungus with good biological activity is concerned by scholars at home and abroad in recent years, but the crude phellinus linteus only stays at the stage of picking wild resources, the wild resources are gradually reduced due to the unregulated collection, and the protection of the existing resources, the optimization of cultivation and fermentation processes and the expansion of the production scale of sporocarp and fermentation production are necessary. It is reported that Ficus hirsuta has the effects of degrading macromolecular polymers and adsorbing heavy metals, and in addition, can be used as a natural dye for dyeing. Therefore, the strain has wide application prospect in the aspects of environmental protection, natural pigment development and the like.
At present, the artificial cultivation mode of the crude chaetomium globosum is immature, the technical problems of low fruiting rate, low yield, poor quality caused by incomplete differentiation of most fruit bodies and the like exist, and the development and utilization of the crude chaetomium globosum are severely restricted.
Disclosure of Invention
Aiming at the defects and shortcomings in the prior art, the invention aims to provide a culture medium of chaetomium globosum, an artificial cultivation method and application, and aims to solve the technical problems of immature chaetomium globosum artificial cultivation technology, low fruiting rate, low yield and poor quality in the prior art.
In order to achieve the purpose, the invention adopts the following technical scheme:
a culture medium for C.hirsutus, which is a liquid culture medium comprising the following components: 100-500 ml of mulberry leaf and mulberry twig leachate, 8-20 g of glucose, 10-20 g of sucrose, 4.0-8.0 g of brown sugar, 5-10 g of beef peptone, 2-5 g of yeast extract, 0.5-1.5 g of monopotassium phosphate, 1.0-2.0 g of magnesium sulfate and 1000ml of water.
The culture medium for the chaetomium globosum is a culture medium, and comprises the following components in percentage by mass: 24% of mixed wood chips, 56% of a mixture of mulberry leaves and mulberry twigs, 10% of bran, 1% of brown sugar, 5% of humus, 1.2% of monopotassium phosphate, 0.8% of magnesium sulfate, 1% of gypsum and 1% of lime; the sum of the mass percentages of the components of the solid raw material is 100 percent.
A culture medium for Inonotus hirsutus, which is a preservation medium, comprises the following components: 10g of mulberry twigs, 10g of bran and 30-50 ml of nutrient solution; the nutrient solution comprises the following components: 10-20 g of glucose, 5-10 g of brown sugar, 5-10 g of beef extract, 0.5-1.5 g of monopotassium phosphate, 1.0-2.0 g of magnesium sulfate and vitamin B24-8 mg and 1000ml of water.
An artificial cultivation method of Ficus hirsuta, which comprises the following steps:
step S41, inoculating the preservation strain of the crude capillary fungus into a solid culture medium for culture to obtain an activated strain;
step S42, inoculating the activated strain into the liquid culture medium for culture to obtain a liquid strain;
step S43, preparing a culture medium according to the formula;
and step S44, diluting the liquid strain obtained in the step S42, inoculating the diluted liquid strain to the culture medium provided in the step S43, and culturing the diluted liquid strain by adopting a light culture and oxygen enrichment culture mode until the chaetomium hirsutum is mature and harvested.
Specifically, in step S44, the liquid seed culture obtained in step S42 is diluted and inoculated to the culture medium provided in step S43, and the liquid seed culture is cultured by light culture and oxygen-enriched culture until the chaetomium hirsutum is mature and harvested.
The method specifically comprises the following steps:
step S441, spawn running: transferring the fungus bag filled with the liquid strains obtained in the step S42 into a fungus growing room, controlling the temperature at 23-26 ℃, keeping the relative humidity of air at 60-70% and the volume fraction of carbon dioxide at 0.08-0.1%, and culturing for 50-60 days under dark conditions until the bag is full of hyphae;
step S442, primordial differentiation: transferring the fungus bags full of hyphae to a mushroom growing room, controlling the temperature at 25-28 ℃, keeping the relative humidity of air at 80-85%, and adopting light culture and oxygen-enriched culture modes to culture until primordium appears around the mouths of the fungus bags;
step S443, mushroom growing management: after primordium appears at the opening of the fungus bag, controlling the temperature to be 25-28 ℃, keeping the relative humidity of air to be 90-95%, and adopting a light culture and oxygen-enriched culture mode to culture until the sporocarp of the chaetomium hirsutum is mature;
and step S444, harvesting.
Specifically, the light cultivation and oxygen-enriched cultivation method in step S442 includes maintaining the volume fraction of carbon dioxide at 0.1% -0.15%, and controlling the illumination intensity at 100-200 lx.
Specifically, the light cultivation and oxygen-enriched cultivation method in step S443 includes maintaining the volume fraction of carbon dioxide at 0.15% -0.2%, and controlling the illumination intensity at 200-300 lx.
Specifically, the method further comprises the step of strain preservation, wherein the specific operation of strain preservation is as follows: the formula of the invention is used for preparing a preservation culture medium, activated strains are inoculated in the preservation culture medium, the culture is carried out until hyphae overgrow the culture medium, and the cultured strains are stored in a refrigerator at 4 ℃.
The artificial cultivation method of the crude capillary fungus is used for cultivating the crude capillary fungus, and the cultivation method adopts light cultivation and oxygen enrichment cultivation modes.
Compared with the prior art, the invention has the following beneficial effects:
(1) the invention adopts liquid strains for inoculation, and has the advantages of vigorous hypha growth, rapid primordium differentiation and short culture time.
(2) The invention takes mulberry leaves, mulberry twigs and wood dust as main raw materials of the culture medium, effectively shortens the production period of the crude phellinus linteus by adjusting the proportion of the components of the formula and optimizing the environmental conditions, improves the yield and lays a foundation for the large-scale cultivation of the crude phellinus linteus.
(3) The method adopts light culture and oxygen-enriched culture to obtain proper culture conditions, the produced crude Inonotus pileus primordium is completely differentiated, the growth vigor is strong, the yield is high, the pileus of the fruiting body has crude hair, and the fruiting body shape of the fruiting body is similar to that of the wild Inonotus pileus.
(4) The crude capillary fungus cultured by the method can produce a large amount of spore powder, and the nutrition and health care value of the crude capillary fungus are effectively improved.
Drawings
FIG. 1 is a view showing the growth of a fruit body of artificially cultivated Inonotus hirsutus according to the present invention.
FIG. 2 is a basidiospore growth diagram of the artificially cultivated chaetomium globosum sporophore.
Detailed Description
The present invention is described in detail below by way of examples, it being necessary to point out here that the following examples are given by way of illustration only and are not to be construed as limiting the scope of the invention, which is intended to be covered by the claims and that insubstantial modifications and adaptations thereof may be made by those skilled in the art in light of the foregoing disclosure.
All starting materials in the present invention, except where specifically indicated, are commercially available and are not further processed, and laboratory instruments are also commercially available.
Fiveleaf coarse fungus: the fruit body has no handle, is semi-circular to approximate to a horseshoe shape, is soft and juicy when fresh, and is light and crisp after being dried; coarse wool with a thickness of 5 mm; dull edge, fluffy.
The collection time of the wild crude chaetomium globosum is 2015 and 7 months, the collection place is the river town of stone spring county and river town of Ankang city, the parasitic plant is mulberry, the wild chaetomium globosum is separated by adopting a tissue separation method, and is purified and cultured on a composite PDA culture medium, the strain is numbered as the crude chaetomium globosum Ih-ZJ906, and the strain is preserved by the edible fungus research institute of the agricultural science research institute of Ankang city.
Leaching solution of mulberry leaves and mulberry twigs: adding granular, dried and mildew-free mulberry leaves and mulberry twigs into 1000mL of water according to the proportion of 1: 3-5, boiling for 30 minutes, and filtering to obtain liquid, namely the mulberry leaf and mulberry twig leaching solution.
The artificial cultivation of the chaetomium globosum is completed according to the following steps:
step S41, preparing a solid culture medium according to a conventional method, wherein the formula is as follows: 200g of potato, 20g of glucose, 6g of beef extract, 1.0g of monopotassium phosphate, 1.0g of magnesium sulfate and vitamin B24mg, agar 12g, water 1000 mL. Inoculating the preserved crude capillary fungus strain into the prepared solid culture medium for activation to obtain an activated strain.
Step S42, preparing a liquid culture medium according to a conventional method, wherein the formula is as follows: 100-500 mL of mulberry leaf and mulberry twig leachate, 15g of glucose, 15g of sucrose, 5.0g of brown sugar, 10g of beef peptone, 2g of yeast extract, 0.5g of monopotassium phosphate, 1.0g of magnesium sulfate and 1000mL of water. And then, selecting the activated strain obtained in the step S41 to inoculate the activated strain into a liquid culture medium, and then putting the activated strain into a constant-temperature shaking incubator with the temperature of 23-26 ℃ and the rotating speed of 150-180 r/min to culture for 8-12 d to obtain the liquid strain.
Step S43, preparing a liquid culture medium according to a conventional method, wherein the liquid culture medium comprises the following components in percentage by mass: 24% of mixed wood dust, 56% of mulberry leaf and mulberry twig (the proportion is 1: 3-5), 10% of bran, 1% of brown sugar, 5% of humus, 1.2% of monopotassium phosphate, 0.8% of magnesium sulfate, 1% of gypsum and 1% of lime.
Under aseptic conditions, the liquid seed culture obtained in step S42 was sterilized with sterile water in a ratio of 1: diluting the culture medium according to the proportion of 4-6, and then uniformly inoculating the diluted liquid strains into the culture medium provided by the step S43; culturing by light culture and oxygen-enriched culture until the Inonotus hirsutus is mature and collected. The method specifically comprises the following steps: filling the culture medium provided in the step S43 into polypropylene dog-ear fungus bags of 17cm multiplied by 33cm multiplied by 0.006cm, wherein each bag is filled with about 450g of the culture medium, and inoculating the diluted liquid strains by an inoculation gun, wherein the inoculation amount of each bag is 15-20 ml; then spawn running is carried out according to the following specific steps: and (3) transferring the fungus bag filled with the liquid strains into a fungus growing room, controlling the temperature at 23-26 ℃, keeping the relative humidity of air at 60-70% and the volume fraction of carbon dioxide at 0.08-0.1%, and culturing for 50-60 days under dark conditions until the bag is full of mycelia.
Primordial differentiation and fruiting management were performed after germination, and the influence of the volume fraction of carbon dioxide and the intensity of light on the growth of chaetoceros hirsutum in the light culture and oxygen-enriched culture modes was observed by examples 1 and 2.
Example 1
Selecting five groups of fungus bags full of hypha, transferring the fungus bags to a mushroom growing room, and in the primordium differentiation stage: controlling the temperature to be 25-28 ℃, keeping the relative humidity of air to be 80-85%, and the illumination intensity to be 100-200 lx, culturing for about 10 days until primordia appear around the bag mouth of the fungus to obtain sporocarp, entering a fruiting management stage, and in the fruiting management stage: controlling the temperature to be 25-28 ℃, keeping the relative humidity of air to be 90% -95%, and the illumination intensity to be 200-300 lx, culturing until the sporocarp is mature, respectively setting different carbon dioxide volume fractions in primordium differentiation and fruiting management stages, recording the primordium differentiation rate and the yield of the crude capillary, and giving the influence of the different carbon dioxide volume fractions on the primordium differentiation rate and the yield of the crude capillary in table 1.
TABLE 1 influence of carbon dioxide volume fraction on the differentiation rate and yield of the protoplasm of Inonotus hirsutus
Example 2
Selecting five groups of fungus bags full of hypha, transferring the fungus bags to a mushroom growing room, and in the primordium differentiation stage: controlling the temperature to be 25-28 ℃, keeping the relative humidity of air to be 80-85%, and the volume fraction of carbon dioxide to be 0.1-0.15%, culturing for about 10 days until primordia appear around the opening of a fungus bag to obtain a fruiting body, then entering a fruiting management stage, and in the fruiting management stage: controlling the temperature at 25-28 ℃, keeping the relative humidity of air at 90-95%, the volume fraction of carbon dioxide at 0.15-0.2%, controlling the illumination conditions and intensities in the primordium differentiation stage and the sporocarp growth stage, recording the primordium differentiation rate and the yield of the crude phellinus linteus, and giving the influence of different illumination intensities on the primordium differentiation rate and the yield of the crude phellinus linteus in table 2.
TABLE 2 Effect of light intensity on the differentiation rate and yield of the protoplasm of Inonotus hirsutus
Example 3
In this embodiment, the method steps described in the present invention are adopted to cultivate chaetomium globosum, and in order to observe the influence of the ratio of the mulberry leaf-mulberry branch mixture to wood chips on the growth and yield of hyphae, when cultivating cultivars, seven groups of cultivation media with different ratios of mulberry leaves-mulberry branches to wood chips are selected for experiments, wherein the mass ratio of mulberry leaves to mulberry branches in the mulberry leaf-mulberry branch mixture is 1: 3-5, and when manufacturing the cultivation media, the cultivation media are added with the mulberry leaf-mulberry branch mixture, wood chips and bran, and other components, the mass percentage of which is 10%, specifically 1% of brown sugar, 5% of humus soil, 1.2% of potassium dihydrogen phosphate, 0.8% of magnesium sulfate, 1% of gypsum, and 1% of lime.
TABLE 3 different proportions of the mixture of mulberry leaves and mulberry branches and wood flour
Then, the following parameters were measured:
average bag full time/d, hypha growth rate/(cm/d) and yield (g/bag). Data processing:
all data were collated using Excel 2010 software and statistical analysis was performed using SPSS 21.0 for one-way anova.
Results and analysis:
TABLE 4 Effect of different cultivar media on the growth of Firmiana hirsuta
Table 4 shows the effect of different cultivar media on the growth of c.hirsutus, as can be seen from table 4: when the culture medium of the formula of the 12 th group is used, the growth rate of the chaetomium hirsutum hyphae is fastest and is 5.481mm/d, the bag filling time is shortest and is 60.2d, the yield is highest and reaches 308.46 g/bag, and the expression of the formula of the 17 th group with the mulberry leaf and mulberry branch content being zero is worst, so that the fact that the mulberry leaf and mulberry branch are added into the culture medium of the cultivated species can improve the yield of the chaetomium hirsutum.
Example 4
In this example, the method of the present invention was used to cultivate chaetomium globosum, in order to observe the influence of the component ratio of the liquid culture medium on the growth and yield of hyphae, five different groups of liquid culture media were selected when cultivating cultivars, the different ratios of the liquid culture media are shown in table 5, and the influence of the different liquid culture media on the dry quality of chaetomium globosum hyphae is shown in table 6.
Quality of dried hyphae: inoculating the activated crude capillary fungus mother strain into a liquid culture medium for culturing for 8-12 days, carrying out suction filtration on mycelium pellets, placing the mycelium pellets in a 65 ℃ oven for drying until the weight is constant, and weighing to obtain the mass which is the dry mass of the mycelium.
TABLE 5 different proportioning test of liquid culture medium
As can be seen from Table 6, the ratio of the liquid medium was different, and the difference in the dry mass of the mycelia was large. In the 21 st group of experiments, the dry mass of the mycelia is the largest and reaches 2.241g/100mL, the dry mass of the mycelia in the 20 th to 22 th groups is obviously higher than that of the mycelia in the 18 th and 19 th groups, and experimental data show that the addition of the mulberry leaf and mulberry twig leachate in a liquid culture medium is beneficial to the accumulation of dry substances of the chaetoceros hirsutus.
TABLE 6 influence of different formulations on the quality of mycelial stem of Inonotus hirsutus
Example 5
Storage Medium optimization test
In this example, the method of the present invention was used to culture chaetomium globosum, and then comparative experiments were performed to determine the effect of different storage media on the hyphal viability of chaetomium globosum.
1. Conventional composite PDA culture medium: 200g of potato, 10g of glucose, 20g of cane sugar, 2g of peptone, 1.0g of monopotassium phosphate, 0.5g of magnesium sulfate, 12g of agar and 1000mL of water.
2. Preservation culture medium: 10g of mulberry twig, 10g of bran, 10-20 g of glucose, 5-10 g of brown sugar, 5-10 g of beef extract, 0.5-1.5 g of monopotassium phosphate, 1.0-2.0 g of magnesium sulfate and vitamin B24-8 mg and 1000ml of water.
The culture media were used to store Inonotus hirsutus, and the mycelia were stored in a refrigerator at 4 ℃ for 1 month, 3 months, and 6 months, respectively, and the results are shown in Table 7.
TABLE 7 Effect of different storage times and storage media on the viability of C.hirsutum hyphae
Note: + + + indicates strong and dense hyphae; the + hyphae grow thicker; + weak hyphal growth.
As can be seen from Table 7, the chaetomium globosum preserved by the preservation medium of the invention has dense hypha growth, little hypha growth rate change, normal hypha activity and no aging phenomenon after 6 months. However, after 3 months, the mycelial growth and growth rate of the coarse chaetomium globosum preserved by using the composite PDA culture medium are weakened, and the aging phenomenon is obviously caused. This indicates that the proper storage medium is a key factor for the viability of the stored hyphae.
Finally, collecting the strains obtained after the test tube activation, wherein the strains have the preservation numbers as follows: CGMCC No.18587, its classification name: fibriella rough, Latin literature name: inonotus hispidus, depository: china general microbiological culture Collection center, address: west road No.1 hospital No. 3, north jing, chaoyang district, with a date of 10 months and 11 days in 2019.
In the later period of the experiment, the pileus of the crude-capillary-fungus sporocarp cultivated by the method has the characteristics that the pileus has crude hair, the shape of the pileus is similar to that of the wild crude-capillary-fungus sporocarp, a large amount of spore powder can be generated, and the nutrition and health care value of the crude-capillary-fungus are effectively improved.
The invention is not limited to the specific embodiments, and any equivalent changes to the technical solution of the invention by reading the specification by those skilled in the art are covered by the claims of the invention.
While the present invention has been described with reference to the embodiments shown in the drawings, the present invention is not limited to the embodiments described above, which are intended to be illustrative rather than restrictive, and it will be understood by those skilled in the art that various changes may be made therein without departing from the spirit and scope of the invention as defined in the appended claims.
Claims (2)
1. An artificial cultivation method of Ficus hirsuta, which is characterized by comprising the following steps:
step 1, inoculating a crude phellinus hirsutus preservation strain to a solid culture medium to obtain an activated strain;
the crude filamentous fungi is preserved in the China general microbiological culture Collection center of China Committee for culture Collection of microorganisms, the number of the preserved strain is Ih-ZJ906, and the preservation time is 2019, 10 months and 11 days;
step 2, inoculating the activated strain obtained in the step 1 into a liquid culture medium for culture to obtain a liquid strain;
the liquid medium comprises the following components: 100-500 ml of mulberry leaf and mulberry twig leachate, 15g of glucose, 15g of cane sugar, 5.0g of brown sugar, 10g of beef peptone, 2g of yeast extract, 0.5g of monopotassium phosphate, 1.0g of magnesium sulfate and 1000ml of water;
step 3, preparing a culture medium, wherein the culture medium comprises the following components in percentage by mass: 24% of mixed wood dust, 56% of a mixture of mulberry leaves and mulberry twigs, 10% of bran, 1% of brown sugar, 5% of humus, 1.2% of monopotassium phosphate, 0.8% of magnesium sulfate, 1% of gypsum and 1% of lime; the sum of the mass percentages of the components of the solid raw materials is 100%, and the mass ratio of the mulberry leaves to the mulberry twigs is 1: 3-5;
step 4, diluting the liquid strain obtained in the step 2, inoculating the diluted liquid strain to the culture medium provided in the step 3, culturing the diluted liquid strain in a light culture and oxygen-enriched culture mode until the Inonotus hirsutus is mature, and harvesting the Inonotus hirsutus;
the method also comprises strain preservation, and the specific operation of the strain preservation is as follows: preparing a preservation culture medium, inoculating an activated strain into the preservation culture medium, culturing until the culture medium is full of mycelia, and storing the cultured strain in a refrigerator at 4 ℃;
the preservation medium comprises the following components: 10g of mulberry twigs, 10g of bran and 30-50 ml of nutrient solution; the nutrient solution comprises the following components: 10-20 g of glucose, 5-10 g of brown sugar, 5-10 g of beef extract, 0.5-1.5 g of monopotassium phosphate, 1.0-2.0 g of magnesium sulfate and vitamin B24-8 mg of water and 1000ml of water;
step 4, the liquid strain obtained in the step 2 is diluted and inoculated to the culture medium provided in the step 3, and is cultured in a light culture and oxygen-enriched culture mode until the chaetomium hirsutum is mature and harvested, and the method specifically comprises the following steps:
step 41, spawn running: transferring the fungus bag containing the liquid strains obtained in the step 2 into a fungus growing room, controlling the temperature to be 23-26 ℃, keeping the relative humidity of air to be 60-70% and the volume fraction of carbon dioxide to be 0.08-0.1%, and culturing for 50-60 days under dark conditions until the bag is full of mycelia;
step 42, primordial differentiation: transferring the fungus bags full of hyphae to a mushroom growing room, controlling the temperature at 25-28 ℃, keeping the relative humidity of air at 80-85%, and adopting light culture and oxygen-enriched culture modes to culture until primordium appears around the mouths of the fungus bags;
step 43, fruiting management: after primordium appears at the opening of the fungus bag, controlling the temperature to be 25-28 ℃, keeping the relative humidity of air to be 90-95%, and adopting a light culture and oxygen-enriched culture mode to culture until the sporocarp of the chaetomium hirsutum is mature;
step 44, harvesting;
step 42, the light culture and oxygen-enriched culture modes comprise that the volume fraction of carbon dioxide is kept to be 0.1-0.15%, and the illumination intensity is controlled to be 100-200 lx;
and 43, the light culture and oxygen enrichment culture modes comprise that the volume fraction of the carbon dioxide is kept to be 0.15-0.2%, and the illumination intensity is controlled to be 200-300 lx.
2. The artificial cultivation method of Firmiana sinensis as claimed in claim 1, which is used for cultivating Firmiana sinensis, and is characterized in that the artificial cultivation method adopts light cultivation and oxygen enrichment cultivation.
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