CN112442451B - Endophytic mycorrhizal fungi for promoting growth of blueberries and application thereof - Google Patents
Endophytic mycorrhizal fungi for promoting growth of blueberries and application thereof Download PDFInfo
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- CN112442451B CN112442451B CN202011532501.6A CN202011532501A CN112442451B CN 112442451 B CN112442451 B CN 112442451B CN 202011532501 A CN202011532501 A CN 202011532501A CN 112442451 B CN112442451 B CN 112442451B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
Abstract
The invention provides an endophytic mycorrhizal fungus for promoting growth of blueberries, wherein the strain is DYD-1 of Phialocephala fortini (Phialophthalla fortini) with the preservation number of CGMCC No.21050, and the application is also provided for promoting the growth of the blueberries. The fungus Phialocephala fortinii (DYD-1) is inoculated to the blueberries to realize mycorrhization, the growth of the blueberries is promoted, the application range of the blueberries is widened after inoculation, the blueberries can grow well under the condition of proper organic matters without regulating acid, and the environment-friendly effect is realized.
Description
Technical Field
The invention belongs to the technical field of strains, and particularly relates to endophytic mycorrhizal fungi for promoting growth of blueberries and application thereof.
Background
The blueberry planting needs special soil environments with high organic matters, high humidity and acidity, the produced soil needs sulfur for adjusting acid, adding organic matters and the like, the production cost is high, the technical requirement is high, and the subsequent utilization and sustainability of the soil are damaged.
Disclosure of Invention
Aiming at the defects of the prior art, the technical problem to be solved by the invention is to provide an endophytic mycorrhizal fungus for promoting the growth of blueberries and an application thereof.
In order to solve the technical problems, the invention adopts the technical scheme that: an endophytic mycorrhizal fungus for promoting growth of blueberries is a fungus strain DYD-1 of Nostoc laterosporus (Phialoca fortini), the preservation unit is the common microorganism center of China Committee of culture Collection of microorganisms, the preservation number is CGMCC No.21050, and the preservation date is 11, 12 days in 2020.
Preferably, the ITS rDNA of the endophytic mycorrhizal fungus is shown in SEQ ID NO 1.
The invention also provides application of the endophytic mycorrhizal fungi for promoting the growth of the blueberries.
Compared with the prior art, the invention has the following advantages:
the fungus (Phialoca fortini) DYD-1 is a new strain, is inoculated on blueberries to realize mycorrhization, promotes the growth of the blueberries, widens the application range of the blueberries after inoculation, can well grow under the condition of proper organic matters without regulating acid, and is an environment-friendly green biological growth promotion method.
The present invention will be described in further detail with reference to the accompanying drawings and examples.
Drawings
FIG. 1 is a diagram of DYD-1 colonies according to example 1 of the present invention.
FIG. 2 is a state diagram of mycorrhization of DYD-1 strain in blueberry root in example 1 of the present invention.
FIG. 3 is a biomass growth diagram of blueberry growth promotion by the endophytic mycorrhizal fungi for promoting blueberry growth.
Detailed Description
Example 1
The endophytic mycorrhizal fungi for promoting the growth of the blueberries, which is the strain DYD-1 of Nostoc laterosporus (Dialocephala fortini), has the preservation unit of China general microbiological culture Collection center (CGMCC) with the preservation number of CGMCC No.21050 and the preservation date of 2020, 11 months and 12 days; the ITS rDNA of the endophytic mycorrhizal fungi is shown as SEQ ID NO 1.
The fungus, named Futing bottlecephala fortini (Dialocephala fortini) DYD-1 in this example is obtained by separating and purifying from wild blueberry roots in spring forest farm in New Qing region of Yichun city, heilongjiang province, and the DYD-1 obtained by separation and purification in this example is inoculated to a peptone agar solid medium for culture, wherein the peptone agar solid medium is prepared from the following raw materials in parts by weight: 10g/L of peptone, 20g/L of maltose and 20g/L of agar. As shown in FIG. 1, the colonies are dark gray, the edges of the colonies are fine (FIG. 1A), and the hyphae penetrate into the medium (FIG. 1B). Under microscope observation, the hyphae were branched and separated in dark color (FIG. 2E, arrow).
The mode of the hypha of the Phialocephala fortinii DYD-1 in the blueberry root planting is shown in figure 2, wherein A in figure 2 is a root cell, a thick spiral structure is formed by transparent thick hypha, and the hypha is blue after being dyed (an arrow in figure 2A). FIG. 2B shows that the hyphae, even after staining, remained light brown (arrows 2B), shuttling between colonized cells (arrows 2C) and within cells (FIG. 2C). Hyphae mainly colonize the outer surface of the root after infection (as shown in FIG. 2D), and dark hyphae with septa among cells enter the epidermal cells of the root and then attach to the inner wall of the cells as shown in FIG. 2F (arrows in FIG. 2F). The hypha sheath is formed by narrow hyaluronic acid hyphae and turns into blue after being dyed. Part of the root system is embedded in a loose net formed by dark diaphragmatic hyphae. These parenchyma tissues (fig. 2D arrows) are typically on the surface of the implanted fine heel, fig. 2D. The samples of FIGS. 2A, 2B, 2C and 2E were stained with Trypan blue and observed by an upright microscope equipped with DIC, and the samples of FIGS. 2D and 2F were observed by a transmission electron microscope.
Example 2
The application of the endophytic mycorrhizal fungus for promoting the growth of blueberries, wherein the endophytic mycorrhizal fungus, phialocephala fortini (DYD-1) is used for promoting the growth of blueberries.
The test plant is a blueberry tissue culture seedling, the variety of the blueberry is Meideng, the test is carried out in an indoor artificial climate incubator, and a conidium liquid pot culture root irrigation inoculation method is adopted.
Adopting root irrigation inoculation method, the seed containing 1 × 10 6 The method comprises the steps of inoculating blueberry tissue culture seedlings with the seedling age of 60 days with a single spore/mL of fungus Phialocephala fortinii (Dialocephala fortinii) DYD-1 conidium suspension, inoculating 5mL of conidium suspension to each blueberry seedling for 3 times, inoculating once every other week to serve as a treatment group, setting 3 repetitions to the treatment group, setting 3 blueberry seedlings for each repetition, and setting a control group at the same time, wherein the blueberry seedlings of the control group are inoculated with the same amount of sterilized distilled water. The culture medium is selected from sterilized moss (shown in fig. 3A and 3C), and is not usedAdjusting pH with sulfur to 6.0. Culturing in an artificial climate room at 25 ℃ (keeping moist by spraying water) for 60 days, completely digging out the whole plant (figure 3B), measuring the nitrogen concentration of each part of the root, stem and leaf of the plant, weighing the fresh weight, the root length, the root surface area and the root volume of the plant, and calculating the biomass growth rate of the ratio of the blueberry seedling inoculated with the fungus Phialocephala fortini (Dialocephala fortini) DYD-1 to the control group (distilled water treatment).
Biomass growth rate (%) =100 × (treatment group biomass-control group biomass)/control group biomass
As can be seen from FIG. 3, DYD-1 of Acetotrichum fornici (Dialocephala fortini) can remarkably promote the increase of biomass of blueberry seedlings, and the growth rate is 26.67 +/-0.94%.
TABLE 1 variation of root length, root surface area, root volume, etc. of Myrtillus familiaris (Pheallocephala fortini) DYD-1 inoculated blueberry seedlings (60 d)
| Sample (I) | Root length (cm) | Root surface area (cm) 2 ) | Root volume (cm) 3 ) |
| Control group | 2056.39±462.26b | 1953.52±271.32b | 280.87±88.87b |
| Treatment group | 4564.58±970.81a | 4339.23±952.62a | 665.58±176.15a |
Note: the letters in the table indicate significance of differences at p < 0.01 levels, as in table 2.
As can be seen from Table 1, DYD-1 of Acidocella fortinii (Dialocephala fortinii) can significantly promote the growth of blueberry roots, the increase of root surface area and root volume.
TABLE 2 Nitrogen concentration of Phosphacephala (Phialocephala fortini) DYD-1 inoculated blueberry seedlings (60 d)
As can be seen from Table 2, DYD-1 of Aconitum forbesii (Phialocephala fortini) can remarkably promote the increase of the nitrogen concentration at the blueberry leaf part, improve the biomass and promote the growth of blueberry seedlings.
The above description is only for the preferred embodiment of the present invention, and is not intended to limit the present invention in any way. Any simple modification, change and equivalent changes of the above embodiments according to the technical essence of the invention are still within the protection scope of the technical solution of the invention.
Sequence listing
<110> village happy science and technology institute of agriculture academy of sciences of Heilongjiang province
<120> endophytic mycorrhizal fungi for promoting growth of blueberries and application thereof
<130> 2020.11.15
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 564
<212> DNA
<213> Artificial Synthesis (Artificial synthesis)
<400> 1
ttccgtaggt gaacctgcgg aaggatcatt acaagtgagg ctaccgaacg ttggaaacag 60
cggttaggag cttacaccca cccgtgtcta catactattg ttgctttggc gggccgtggc 120
ctccactgcg ggctctgctc gtgtgtgccc gccagagaac caaactctga atgttagtga 180
tgtctgagta ctatctaata gttaaaactt tcaacaacgg atctcttggt tctggcatcg 240
atgaagaacg cagcgaaatg cgataagtaa tgtgaattgc agaattcagt gaatcatcga 300
atctttgaac gcacattgcg ccctgtggta ttccgcaggg catgcctgtt cgagcgtcat 360
ttaaccactc acgcctagcg tggtattggg gcacgcggtc tccgcggccc tcaaaattag 420
tggcggcgcc ggtgggctct aagcgtagta catactcccg ctatagagtt cccccggtgg 480
ctcgctagaa cccccaattt ttacaggttg acctcggatc aggtagggat acccgctgaa 540
cttaagcata tcaataagcg gagg 564
Claims (2)
1. An endophytic mycorrhizal fungus for promoting growth of blueberries, wherein the endophytic mycorrhizal fungus strain is Ustilaginoides formosanus (Dialocephala fortini) DYD-1, the preservation unit is China general microbiological culture Collection center, the preservation number is CGMCC No.21050, and the preservation date is 11 months and 12 days 2020; the ITS rDNA of the endophytic mycorrhizal fungi is shown as SEQ ID NO 1.
2. Use of an endophytic mycorrhizal fungus for promoting growth of blueberries according to claim 1, wherein the endophytic mycorrhizal fungus is used for promoting growth of blueberries.
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| WO2020251379A1 (en) * | 2019-06-11 | 2020-12-17 | Uniwersytet Jagielloński | Inoculum, a method of preparing inoculum and a method of biotization of american blueberry |
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| CN101486970A (en) * | 2008-12-01 | 2009-07-22 | 浙江大学 | Fungus strain and uses thereof |
| JP2013042695A (en) * | 2011-08-24 | 2013-03-04 | Ibaraki Univ | Production method of asparagus seedling utilizing root endophyte (dse) phialocephala fortinii |
| CN107872955A (en) * | 2014-12-29 | 2018-04-03 | Fmc有限公司 | Beneficial to the bacillus licheniformis RTI184 compositions and its application method of plant growth |
| CN111184031A (en) * | 2018-11-15 | 2020-05-22 | 浙江省大盘山国家级自然保护区管理局 | Liquid composite mycorrhizal inoculant for promoting growth of blueberry seedlings and preparation method thereof |
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