CN104004666A - Endophytic fungi capable of promoting China fir growth - Google Patents
Endophytic fungi capable of promoting China fir growth Download PDFInfo
- Publication number
- CN104004666A CN104004666A CN201410246127.1A CN201410246127A CN104004666A CN 104004666 A CN104004666 A CN 104004666A CN 201410246127 A CN201410246127 A CN 201410246127A CN 104004666 A CN104004666 A CN 104004666A
- Authority
- CN
- China
- Prior art keywords
- growth
- strain
- cylindrocarpon
- china fir
- endophytic fungi
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention provides endophytic fungi capable of promoting China fir growth, and provides methods for separating, purifying, inoculating, culturing and applying the endophytic fungi. The endophytic fungi is a Cylindrocarpon sp. CG2 bacterial stain, and is registered and preserved in the China General Microbiological Culture Collection Center on May 20, 2014 with a preservation number of CGMCC NO.9188. The bacterial strain is separated and purified from a China fir plant and inoculated to a China fir tissue cultured seedling, comprehensive evaluation on each index of photosynthesis and biological characteristics further proves that the endophytic fungi has an effect of promoting plant growth, and the endophytic fungi with a beneficial function of promoting plant growth can be applied to production.
Description
Technical field
This invention relate to the endogenetic fungus that a strain can promote Growth of Chinese Fir (
cylindrocarpon sp.), comprise that its separation, purification, inoculation and cultivation connect and application method.
Background technology
The research of endophyte of plant started from for 19 end of the centurys, and Vogl isolates the first strain endophyte from rye grass Lolium temulentum L. seed.But the endophyte really starting in large quantity research plant originates in the eighties in last century, be mainly to conduct a research in the vegetation of Temperate Region in China, subtropical zone and torrid areas.
Forefathers can isolate endophyte from majority of plant, therefore can infer that endophyte is ubiquitous in plant.In worldwide, at least in the gramineae farm crop of more than 80 genus kind more than 290, find endophyte.The unique function that the endomycorrhiza separating from plant at present has according to it can be divided into: the plant growth-promoting bacterias such as vinelandii, solid potassium bacterium, solid phosphorus bacterium, have the biocontrol microorganisms of disease and insect resistance and have the resistance bacterium that promotes the repair ability of plant to poor environment.
Xie Qingmei and Huang Jianhe (1985) morphological specificity to China fir endomycorrhiza and the qualification of mycorrhizal fungi, mainly do anatomy analysis and the mycelia obtaining from Rhizosphere Soil with wet screening-decantation and chlamydospore with the symbiotic structure of Chinese fir root bark layer tissue and endomycorrhiza fungi and observe.Result shows, according to chlamydosporic Morphologic Characteristics in the anatomy analysis of China fir endomycorrhiza and rhizosphere soil, determines that China fir endomycorrhiza is to belong to one clump of branch type endomycorrhiza of vesicle (being called for short VA mycorhiza).Further research China fir endomycorrhiza, to the growing and the effect of resistance aspect of China fir, adequately and reasonably utilizes endomycorrhiza preparation to promote the problem of Growth of Chinese Fir, has great economic worth.Particularly, in the barren red soil region of south China nutrient, research endomycorrhiza strengthens the absorption of Phosphorus in Soil, is major issue urgently to be resolved hurrily in the China fir fast growing of current Red Soil Region of South China.In summary, only carry out identification research from root position about the research of the endogenetic fungus aspect of China fir, and Lignum seu Ramulus Cunninghamiae Lanceolatae leaf position and stem position are not all related to the research of endogenetic fungus.In addition, about endogenetic fungus also exists blank on the impact research of China fir self growth.
Summary of the invention
The object of the present invention is to provide a strain that the endogenetic fungus of Growth of Chinese Fir, the endogenetic fungus separation, purification, cultivation and the application method thereof that provide a strain can promote Growth of Chinese Fir can be provided.
A strain provided by the invention can promote the endogenetic fungus (Cylindrocarpon of Growth of Chinese Fir
sp.), obtaining from Root-bark of Chinese Fir part from, purifying, its bacterium colony is soft fine hair shape, sporodochium mode and produces spore.Bottle stalk is cylindrical, has open apical pore and short neck shape thing, therefrom produces the conidium of cement shape colourless, that wall is smooth.Macroconidium, cylindrical, the blunt circle in two ends, bending, has a diaphragm.This fungi is Cylindrocarpon (Cylindrocarpon
sp.) CG2 bacterial strain, registered preservation at China Committee for Culture Collection of Microorganisms's common micro-organisms center on May 20th, 2014, address is No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, preserving number is CGMCC NO.9188.
For achieving the above object, the present invention adopts following technical scheme:
Separation, the purifying of this bacterium comprise:
A, material: the root, stem section, the leaf that collect different year are divided into three parts, clean up with tap water, point install in valve bag, in 4 DEG C of Refrigerator stores;
---sterilized water embathes once---10% clorox soaks 7min---, and sterilized water embathes once for B, sterilization: 70% alcohol-pickled 30s.Whether the water after sample embathes is for the last time contained in the small beaker of sterilizing, at flat lining out in contrast, thorough with the sterilization of check sample, after some days, generates if any bacterium colony, shows that sample surfaces sterilization is not thorough, needs to continue to adjust sterilization method;
The selection of C, inoculation position: leaf tip, leaf central part and 3 processing of leaf base; Branch: upper, middle and lower is divided into 3 positions, wherein the 3rd Wei Zhijing junction, position; Root: be divided into 2 parts of the tip of a root and foundation;
D, inoculation: the explant after sterilization is cut with vaccinating lancet, be laid in media surface, observe colony growth situation cultivate 5-7 days in 28 DEG C of constant incubators after.Some bacterial strain breedings rapidly, can first be carried out separation and purification by the bacterial strain of its generation; The bacterial strain of poor growth and comparatively small amt can extend observing time, until the stable rear purifying of its growth.
Solid culture: use improvement Martin solid medium, formula for peptone 5.0g/L, yeast extract powder 2.0g/L, glucose 20.0g/L, dipotassium hydrogen phosphate 1.0g/L, magnesium sulfate 0.5g/L, agar 14.0g/L, other is sterilized water, pH value 6.4 ± 0.2, culture temperature is 28 DEG C, training method is dull and stereotyped cultivation, and incubation time is 48h;
E, different types of endogenetic fungus access plate culture medium that separation is bred carry out purifying, obtain the above-mentioned Cylindrocarpon of single culture after 2-3 point connects purifying, switching
sp.function stem;
Point inoculation purifying: the transfering loop after sterilization is slightly clicked to thalline surface, then clicked inoculating surfaces, the point that the method is more traditional is planted culture method inoculum size more easy to control, thus inoculation efficiency and recall rate to endogenetic fungus improved.
The preparation method of above-mentioned a kind of endogenetic fungus application bacterium liquid that can promote Growth of Chinese Fir, by above-mentioned bacterial strain access liquid nutrient medium, shaking table shaking culture, culture temperature is 28 DEG C, incubation time 48-72h, utilize blood counting chamber to calculate bacterial concentration, bacterium liquid is diluted to 1.0-9.0 × 10 with ultrapure water
6it is for subsequent use that cfu/ml is finished product; Liquid nutrient medium: be improvement Martin substratum, wherein peptone 5.0g/L, yeast extract powder 2.0g/L, glucose 20.0g/L, dipotassium hydrogen phosphate 1.0g/L, magnesium sulfate 0.5g/L, other is sterilized water, pH value 6.4 ± 0.2.
The application method of above-mentioned a kind of endogenetic fungus application bacterium liquid that can promote Growth of Chinese Fir, is the bacterium liquid of this bacterial strain of application, and transplanted seedling tree dips in root and waters root for forest land early stage.
The application method of above-mentioned a kind of endogenetic fungus application bacterium liquid that can promote Growth of Chinese Fir is with 9.0 × 10
5cfu/ml bacterium liquid, waters in the rhizosphere of every strain China fir in forest land by every strain 100ml.
The application method of above-mentioned a kind of endogenetic fungus application bacterium liquid that can promote Growth of Chinese Fir is the bacterium liquid 5.0 × 10 of this bacterial strain of application
5cfu/ml dips in seedling root 10min before Rooted Cuttings is planted.
The bacterial strain the present invention relates to separation and purification from China fir plant obtains, through being inoculated in China fir tissue cultured seedling, comprehensively pass judgment on according to the indices of photosynthesis and biological characteristics, further confirm that it has promoter action to plant strain growth, the useful function endogenetic fungus searching out promoting plant strain growth can be applicable to produce.
Bacterial strain CG2 is 11.44% in the rate of increase of watering the height of seedling to plant under root mode (R), is 10.87% to the rate of increase of leading thread; Being 15.03% in the rate of increase of drenching the height of seedling to plant under hat mode (G), is 12.73% to the rate of increase of leading thread.And the height of seedling of control group plant and the rate of increase of leading thread are respectively 7.31% and 6.94%.Under above-mentioned two kinds of inoculation methods, the growth-promoting functions of CG2 is all very remarkable.
From root/shoot ratio index, the root/shoot ratio under G processes is greater than R and processes, and the root/shoot ratio of inoculation CG2 seedling is processed respectively than contrast high 17.76% and 10.82% in G processing and R.From biomass indexes, the biomass under G processes is but less than R to be processed, and the root/shoot ratio of inoculation CG2 seedling is processed respectively than contrast high 64.29% and 74.16% in G processing and R.To sum up analysis, no matter CG2 bacterial strain is the growth that all more can promote root system under G processing or R processing than contrast, and the growth-promoting effect that G processes is better than R processing.
The above-ground plant parts of inoculating strain CG2 and the nitrogen content of underground part are all high than control group; Under G processes, difference high 17.99% and 23.79%, R process lower difference high 23.84% and 6.32%.The over-ground part of inoculating strain seedling and the carbon content of underground part be difference high 10.62% and the lower difference high 9.56% and 7.05% of 8.28%, R processing under G processes.
Brief description of the drawings
The impact of Fig. 1 endogenetic fungus CG2 on China Fir Seedling biomass and root/shoot ratio.
Embodiment
embodiment 1
1, the application in water planting breeding
Get the above-mentioned endogenetic fungus application bacterium liquid preparing, make 5.0 × 10
5cfu/ml bacterium liquid dips in root 10min before tissue cultured seedling is planted.Can improve and plant seedlings surviving rate more than 10%.
2, rhizosphere soil waters and executes application
This test adopts earth culture pot experiment, tests the China fir tissue cultured seedling that China Fir Seedling used provides for forest-science academy of Fujian Province.Select the consistent nursery stock of growing way to be colonizated in diameter 15cm in May, 2013, in the plastic tub of high 10cm.Soil used is the yellow soil that strictly passes through fumigation.Through weighing, every basin is put into the 3kg soil of equivalent.Through bimestrial restorative growth, continuous three days (July 5, July 6 and July 7) apply the 100mL bacterium liquid of same concentrations respectively at China fir rhizosphere (R) and tree crown (G).Totally 5 dominant bacterias that utilize above-mentioned test to obtain, 4 repetitions of every kind of bacterium liquid, use the aqueous solution as blank.Inoculate the mensuration of carrying out indices after 60 days.
Bacterium solution preparation: strains tested is accessed to the liquid nutrient medium of 50mL, the cultivation of process 72h in constant-temperature shaking incubator.Press decimal dilution method by the dilution of bacterium liquid by stroke-physiological saline solution, utilize blood counting chamber to calculate bacterial concentration and be mixed with 5.5 × 10
6cfu/mL.
Height of seedling leading thread is measured
Steel ruler is measured height of seedling, and electronic digital indicator is measured leading thread.
Biomass estimation
By the different sites of plant respectively label put into the aluminium box of known weight (W1), put into 105 DEG C, baking oven and dry half an hour (deactivating enzyme), at 70 DEG C, dry, after 10h, taking-up aluminium box is put into loft drier and is cooled to room temperature, after weighing, put into baking oven, replication is until constant weight again.Claim to obtain the gross weight (W2) of aluminium box and sample, sample dry weight is W2-W1.
In this test, root/shoot ratio index adopts the mass ratio of plant dry-matter.Below ground, is mainly root system, is underground part; More than ground, being mainly stem and leaf, is over-ground part.
Plant carbon content and nitrogen analysis
Plant carbon content and total nitrogen content adopt CHNS elemental analyser (German Elementar company) to measure, and each sample repeats to survey twice.
2.4 data processing
Data preparation and preliminary treatment adopt Excel2003, and mapping adopts Origin8.0, and the statistical analysis of data adopts SPSS18.0.
Results and analysis
The impact of different strain on China Fir Seedling height of seedling and leading thread
As shown in Table 1, from different treatment angle analysis, employing is watered root mode (R) and the promoter action most applications of plant-growth is greater than to the promoter action of drenching hat mode (G).Bacterial strain CG2 is 11.44% in the rate of increase of watering the height of seedling to plant under root mode (R), is 10.87% to the rate of increase of leading thread; Being 15.03% in the rate of increase of drenching the height of seedling to plant under hat mode (G), is 12.73% to the rate of increase of leading thread.And the height of seedling of control group plant and the rate of increase of leading thread are respectively 7.31% and 6.94%.But under above-mentioned two kinds of inoculation methods, the growth-promoting functions of CG2 is all very remarkable.
The impact of the different endogenetic fungus of table 1 on China Fir Seedling height of seedling and leading thread
Note: in table, data are the means standard deviation of four repetitions.
The impact of different strain on China Fir Seedling root/shoot ratio and biomass
As shown in Figure 1, compared with the control, the seedling root/shoot ratio of inoculating strain CG2 and biomass are drenching hat mode (G) and are watering and under two kinds of processing of root mode (R), all want large.From root/shoot ratio index, the root/shoot ratio under G processes is greater than R and processes, and the root/shoot ratio of inoculation CG2 seedling is processed respectively than contrast high 17.76% and 10.82% in G processing and R.From biomass indexes, the biomass under G processes is but less than R to be processed, and the root/shoot ratio of inoculation CG2 seedling is processed respectively than contrast high 64.29% and 74.16% in G processing and R.To sum up, no matter CG2 bacterial strain is the growth that all more can promote root system under G processing or R processing than contrast, and the growth-promoting effect that G processes is better than R processing.
The impact of different strain on China Fir Seedling plant carbon nitrogen content
As can be seen from Table 2, the above-ground plant parts of inoculating strain CG2 and the nitrogen content of underground part are all high than control group; Under G processes, difference high 17.99% and 23.79%, R process lower difference high 23.84% and 6.32%.The over-ground part of inoculating strain seedling and the carbon content of underground part be difference high 10.62% and the lower difference high 9.56% and 7.05% of 8.28%, R processing under G processes.
The impact of table 2 different strain on China Fir Seedling plant carbon nitrogen content
Note: in table, data are the means standard deviation of four repetitions.
The present invention finds that a strain can promote the endogenetic fungus of Growth of Chinese Fir, this strain China fir endogenetic fungal bacterial strain is adopted to water root and drench two kinds of methods of hat be inoculated in China Fir Seedling.By inoculating the determination test of rear plant, the performance that draws height of seedling leading thread, root/shoot ratio, biomass and the carbon nitrogen content of bacterial strain is all better than contrast, shows that it is for promoting that Growth of Chinese Fir has very large function, and the vaccination ways that waters root is better than drenching hat.
The foregoing is only preferred embodiment of the present invention, all equalizations of doing according to the present patent application the scope of the claims change and modify, and all should belong to covering scope of the present invention.
Claims (3)
1. a strain can promote the endogenetic fungus of Growth of Chinese Fir, it is characterized in that: this fungi is Cylindrocarpon (Cylindrocarpon
sp.) CG2 bacterial strain, registered preservation at China Committee for Culture Collection of Microorganisms's common micro-organisms center on May 20th, 2014, preserving number is CGMCC NO.9188.
2. a strain as claimed in claim 1 can promote the application bacterium liquid of the endogenetic fungus of Growth of Chinese Fir, it is characterized in that: described application bacterium solution preparation method is, by Cylindrocarpon (Cylindrocarpon sp.) CG2 bacterial strain access liquid nutrient medium, shaking table shaking culture, culture temperature is 28 DEG C, incubation time 48-72h, utilizes blood counting chamber to calculate bacterial concentration, and bacterium liquid is diluted to 1.0-9.0 × 106cfu/ml with ultrapure water, and to be finished product for subsequent use; Liquid nutrient medium: be improvement Martin substratum, wherein peptone 5.0g/L, yeast extract powder 2.0g/L, glucose 20.0g/L, dipotassium hydrogen phosphate 1.0g/L, magnesium sulfate 0.5g/L, other is sterilized water, pH value 6.4 ± 0.2.
3. a strain as claimed in claim 2 can promote the application bacterium liquid of the endogenetic fungus of Growth of Chinese Fir to water the application in executing in water planting breeding and rhizosphere soil.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410246127.1A CN104004666B (en) | 2014-06-05 | 2014-06-05 | One strain can promote the endogenetic fungus of Growth of Chinese Fir |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410246127.1A CN104004666B (en) | 2014-06-05 | 2014-06-05 | One strain can promote the endogenetic fungus of Growth of Chinese Fir |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104004666A true CN104004666A (en) | 2014-08-27 |
| CN104004666B CN104004666B (en) | 2016-01-06 |
Family
ID=51365576
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410246127.1A Expired - Fee Related CN104004666B (en) | 2014-06-05 | 2014-06-05 | One strain can promote the endogenetic fungus of Growth of Chinese Fir |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104004666B (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104762219A (en) * | 2015-04-14 | 2015-07-08 | 福建农林大学 | Endophytic fungus for promoting biomass growth of aleurites montana in low-phosphorus environment |
| CN104818217A (en) * | 2015-04-14 | 2015-08-05 | 福建农林大学 | Endophytic fungus capable of promoting biomass growth of aleurites montana |
| CN110564645A (en) * | 2019-09-18 | 2019-12-13 | 嘉兴学院 | Chlorella endophyte and application thereof in promoting growth of chlorella |
| CN111004727A (en) * | 2019-12-25 | 2020-04-14 | 福建农林大学 | Endophytic fungus Z1 for increasing biomass of casuarina equisetifolia in high-salt environment |
| CN107988087B (en) * | 2017-12-29 | 2020-12-11 | 华南农业大学 | Blueberry endophytic fungus with growth promoting effect and application thereof |
-
2014
- 2014-06-05 CN CN201410246127.1A patent/CN104004666B/en not_active Expired - Fee Related
Non-Patent Citations (5)
| Title |
|---|
| JOSE G. MACIA-VICENTE 等: "Colonization of barley roots by endophytic fungi and their reduction of take-all caused by Gaeumannomyces graminis var. tritici", 《CAN.J.MICROBIOL.》, 31 December 2008 (2008-12-31), pages 600 - 609 * |
| 柯海丽 等: "野生五唇兰根部内生真菌多样性研究", 《生物多样性》, vol. 15, no. 5, 31 December 2007 (2007-12-31), pages 456 - 462 * |
| 王亚军 等: "外生菌根真菌对杉木的接种效应", 《西北植物学报》, vol. 26, no. 9, 31 December 2006 (2006-12-31), pages 1900 - 1904 * |
| 谢卿楣 等: "杉木内生菌根的研究", 《福建林学院学报》, vol. 5, no. 2, 30 November 1985 (1985-11-30), pages 82 - 84 * |
| 邓祖军 等: "红树林植物桐花树内生真菌主要无孢类群的分子鉴定", 《海洋学报》, vol. 32, no. 2, 31 March 2010 (2010-03-31), pages 161 - 167 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104762219A (en) * | 2015-04-14 | 2015-07-08 | 福建农林大学 | Endophytic fungus for promoting biomass growth of aleurites montana in low-phosphorus environment |
| CN104818217A (en) * | 2015-04-14 | 2015-08-05 | 福建农林大学 | Endophytic fungus capable of promoting biomass growth of aleurites montana |
| CN104818217B (en) * | 2015-04-14 | 2018-05-08 | 福建农林大学 | One plant can promote the endogenetic fungus that aleurite montana biomass increases |
| CN104762219B (en) * | 2015-04-14 | 2018-05-11 | 福建农林大学 | One plant of endogenetic fungus for promoting aleurite montana biomass to increase under low-phosphorous environment |
| CN107988087B (en) * | 2017-12-29 | 2020-12-11 | 华南农业大学 | Blueberry endophytic fungus with growth promoting effect and application thereof |
| CN110564645A (en) * | 2019-09-18 | 2019-12-13 | 嘉兴学院 | Chlorella endophyte and application thereof in promoting growth of chlorella |
| CN110564645B (en) * | 2019-09-18 | 2020-12-01 | 嘉兴学院 | Chlorella endophyte and application thereof in promoting growth of chlorella |
| CN111004727A (en) * | 2019-12-25 | 2020-04-14 | 福建农林大学 | Endophytic fungus Z1 for increasing biomass of casuarina equisetifolia in high-salt environment |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104004666B (en) | 2016-01-06 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101974438B (en) | Eucalyptus endophyte and application thereof | |
| CN102696466B (en) | Method for quick mycorhiza formation of azalea aseptic seedlings | |
| CN104805019B (en) | One plant of endogenetic fungus that can promote aleurite montana Nutrient Absorption | |
| CN104004666B (en) | One strain can promote the endogenetic fungus of Growth of Chinese Fir | |
| CN102703333B (en) | Mycorrhizal fungi strain efficiently growing with rhododendron seedling | |
| CN103173364B (en) | Endophytic fungus promoting casuarina equisetifolia biomass growth | |
| CN103173359B (en) | Endophytic fungus promoting casuarina equisetifolia root system growth effect | |
| CN104004667B (en) | One strain can promote the endogenetic fungus that Phosphorus In Chinese Fir absorbs | |
| CN104762219A (en) | Endophytic fungus for promoting biomass growth of aleurites montana in low-phosphorus environment | |
| CN104004664B (en) | One strain can improve the photosynthetic endogenetic fungus of China fir | |
| CN103173361B (en) | Endophytic fungus promoting casuarina equisetifolia nutrient element absorption | |
| CN102007869B (en) | Ectomycorrhizal fungi efficiently symbiotic with pinus koraiensis | |
| CN103173360B (en) | Endophytic fungus increasing casuarina equisetifolia chlorophyll content | |
| CN103173362B (en) | Endophytic fungus promoting casuarina equisetifolia photosynthesis | |
| CN101993827B (en) | Functional endophytic fungus for prompting photosynthesis of eucalypt and application thereof | |
| CN104789481A (en) | Endophytic fungus for promoting growth and photosynthesis enhancement of aleurites montana in low-phosphorus environment | |
| CN106010984B (en) | One plant of endogenetic fungus for promoting acacia confusa biomass to increase under low-phosphorous environment | |
| CN103194396B (en) | Aspergillus strain capable of promoting root growth of Casuarina | |
| CN104004665B (en) | One strain can alleviate the China fir endogenetic fungus of P deficiency | |
| CN104818219B (en) | One plant of endogenetic fungus for promoting aleurite montana root growth under low-phosphorous environment | |
| CN115948254B (en) | Trichoderma viride 192-45 and application thereof | |
| CN105969672A (en) | Mixed endophytic fungi capable of promoting growth of heights and ground diameters of Taiwan acacia seedlings | |
| CN106085872A (en) | A kind of mixing endogenetic fungus that can promote acacia confusa Nutrient Absorption | |
| CN104818218B (en) | One plant can promote the endogenetic fungus that aleurite montana phosphorus absorbs | |
| CN105861335B (en) | The endogenetic fungus of one plant of promotion acacia confusa Nutrient Absorption under low-phosphorous environment |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160106 Termination date: 20210605 |