CN103194396B - Aspergillus strain capable of promoting root growth of Casuarina - Google Patents
Aspergillus strain capable of promoting root growth of Casuarina Download PDFInfo
- Publication number
- CN103194396B CN103194396B CN201310068757.XA CN201310068757A CN103194396B CN 103194396 B CN103194396 B CN 103194396B CN 201310068757 A CN201310068757 A CN 201310068757A CN 103194396 B CN103194396 B CN 103194396B
- Authority
- CN
- China
- Prior art keywords
- aspergillus
- root
- strain
- root growth
- casuarina
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 230000002786 root growth Effects 0.000 title claims abstract description 25
- 241000228212 Aspergillus Species 0.000 title claims abstract description 17
- 241000219501 Casuarina Species 0.000 title abstract description 6
- 230000001737 promoting effect Effects 0.000 title abstract description 3
- 241000233866 Fungi Species 0.000 claims abstract description 27
- 241000228257 Aspergillus sp. Species 0.000 claims abstract description 7
- 241000219503 Casuarina equisetifolia Species 0.000 claims description 37
- 241000894006 Bacteria Species 0.000 claims description 28
- 230000001580 bacterial effect Effects 0.000 claims description 26
- 239000007788 liquid Substances 0.000 claims description 22
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 16
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 8
- 235000015097 nutrients Nutrition 0.000 claims description 6
- 244000005700 microbiome Species 0.000 claims description 5
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 4
- 239000001888 Peptone Substances 0.000 claims description 4
- 108010080698 Peptones Proteins 0.000 claims description 4
- 229940041514 candida albicans extract Drugs 0.000 claims description 4
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 4
- 239000008103 glucose Substances 0.000 claims description 4
- 230000006872 improvement Effects 0.000 claims description 4
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 4
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 4
- 235000019319 peptone Nutrition 0.000 claims description 4
- 239000000843 powder Substances 0.000 claims description 4
- 239000012138 yeast extract Substances 0.000 claims description 4
- 239000008280 blood Substances 0.000 claims description 3
- 210000004369 blood Anatomy 0.000 claims description 3
- 238000011534 incubation Methods 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims description 3
- 238000004321 preservation Methods 0.000 claims description 3
- 229910021642 ultra pure water Inorganic materials 0.000 claims description 3
- 239000012498 ultrapure water Substances 0.000 claims description 3
- 230000000694 effects Effects 0.000 abstract description 4
- 238000011156 evaluation Methods 0.000 abstract 1
- 238000009629 microbiological culture Methods 0.000 abstract 1
- 241000196324 Embryophyta Species 0.000 description 27
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 11
- 238000000034 method Methods 0.000 description 11
- 238000012545 processing Methods 0.000 description 11
- 238000011160 research Methods 0.000 description 10
- 239000002689 soil Substances 0.000 description 10
- 230000012010 growth Effects 0.000 description 8
- 238000011081 inoculation Methods 0.000 description 8
- 230000001954 sterilising effect Effects 0.000 description 7
- 238000012360 testing method Methods 0.000 description 7
- 238000004659 sterilization and disinfection Methods 0.000 description 6
- 238000005520 cutting process Methods 0.000 description 5
- 239000002609 medium Substances 0.000 description 5
- 238000000926 separation method Methods 0.000 description 4
- 239000007787 solid Substances 0.000 description 4
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 3
- 230000003321 amplification Effects 0.000 description 3
- 238000003199 nucleic acid amplification method Methods 0.000 description 3
- 239000003643 water by type Substances 0.000 description 3
- 239000002028 Biomass Substances 0.000 description 2
- 241000187809 Frankia Species 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000001543 one-way ANOVA Methods 0.000 description 2
- 229910052698 phosphorus Inorganic materials 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
- 229920003023 plastic Polymers 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 230000004044 response Effects 0.000 description 2
- 229960005486 vaccine Drugs 0.000 description 2
- 239000002023 wood Substances 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- 235000003826 Artemisia Nutrition 0.000 description 1
- 235000003261 Artemisia vulgaris Nutrition 0.000 description 1
- 240000006891 Artemisia vulgaris Species 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 241000219500 Casuarinaceae Species 0.000 description 1
- 241000222199 Colletotrichum Species 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 244000048927 Lolium temulentum Species 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 241000237502 Ostreidae Species 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 241000209504 Poaceae Species 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 241000589516 Pseudomonas Species 0.000 description 1
- 241000589771 Ralstonia solanacearum Species 0.000 description 1
- 241001185310 Symbiotes <prokaryote> Species 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 238000000540 analysis of variance Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 235000009052 artemisia Nutrition 0.000 description 1
- 235000015241 bacon Nutrition 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 230000000443 biocontrol Effects 0.000 description 1
- 229930002875 chlorophyll Natural products 0.000 description 1
- 235000019804 chlorophyll Nutrition 0.000 description 1
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 230000000249 desinfective effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 229910001873 dinitrogen Inorganic materials 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 230000024346 drought recovery Effects 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 230000007773 growth pattern Effects 0.000 description 1
- 239000011121 hardwood Substances 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 230000024121 nodulation Effects 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 235000020636 oyster Nutrition 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 238000007747 plating Methods 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 1
- 239000004575 stone Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000008399 tap water Substances 0.000 description 1
- 235000020679 tap water Nutrition 0.000 description 1
- RTKIYNMVFMVABJ-UHFFFAOYSA-L thimerosal Chemical compound [Na+].CC[Hg]SC1=CC=CC=C1C([O-])=O RTKIYNMVFMVABJ-UHFFFAOYSA-L 0.000 description 1
- 229940033663 thimerosal Drugs 0.000 description 1
- 238000012549 training Methods 0.000 description 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to an aspergillus strain capable of promoting root growth of Casuarina. The strain is Casuarina rhizosphere fungi 63 of Aspergillus sp., and is registered and preserved in China General Microbiological Culture Collection Center on June 28, 2012, with an accession No. being CGMCC No. 6311. The Casuarina rhizosphere fungi 63 is inoculated on hydroponic seedlings of the Casuarina, and a comprehensively evaluation is carried out according to various indicators of root growth, and promotion effects of the fungi on root growth is further confirmed, thereby finding a functional endophytic fungi which is benefit to promote root growth and can be applied in production.
Description
Technical field
The present invention relates to the Aspergillus strain that a strain can promote Horsetail Beefwood root growth.
Background technology
The research of endophyte of plant started from for 19 end of the centurys, and Vogl isolates the first strain endophyte from rye grass Lolium temulentum L. seed
[1].But the endophyte really starting in large quantity research plant originates in the eighties in last century, be mainly to conduct a research in the vegetation of Temperate Region in China, subtropical zone and torrid areas.
Forefathers can isolate endophyte from majority of plant, therefore can infer that endophyte is ubiquitous in plant.In worldwide, at least in the gramineae farm crop of more than 80 genus kind more than 290, find endophyte
[2].The unique function that the endomycorrhiza separating from plant at present has according to it can be divided into: the plant growth-promoting bacterias such as vinelandii, solid potassium bacterium, solid phosphorus bacterium
[3 ~ 4], there is the biocontrol microorganisms of disease and insect resistance
[5 ~ 8]with the resistance bacterium with the repair ability of promotion plant to poor environment.
Casuarinaceae plant is the symbiotrophy type plant that has Frankia bacterium, endophyte root fungus and ectotrophic mycorrhiza concurrently, therefore, about at present more frankia, Cong Zhizhuan mycorhiza (being called for short AM mycorhiza), Ralstonia solanacearum, the blue or green withered false pseudomonas bacillus etc. of relating to of research direction of Horsetail Beefwood mycology aspect, although the growth-promoting effect after Horsetail Beefwood artificial inoculation VA Mycorrhizal Fungi is unquestionable, but only stagnate in the research of root nodule bacterium root fungus, the research of Horsetail Beefwood endogenetic fungus aspect there is not yet report
[9-10].In forefathers' research, apply bacterial strain, mostly be external fungi, also do not remove to find endogenetic fungus from the basic factor that promotes root growth simultaneously, improve its science and reliability
[11].Confirmation can promote the endophyte aspect of root growth to there is not yet report.
Summary of the invention
The object of the present invention is to provide a strain can promote the Aspergillus strain of Horsetail Beefwood root growth.
A strain provided by the invention can promote the Aspergillus strain of Horsetail Beefwood root growth, for aspergillus (
aspergillus sp.) Horsetail Beefwood Flora of Rhizosphere Fungi 63, registered preservation at China Committee for Culture Collection of Microorganisms's common micro-organisms center on June 28th, 2012, deposit number is CGMCC No. 6311.
Separation, the purifying of this bacterium comprise:
A, material: the root, branch, the phyllade sprig that collect different year are divided into three parts, clean up with tap water, point install in valve bag, in 4 ℃ of Refrigerator stores;
---sterilized water embathes once---10% clorox soaks 7min---, and sterilized water embathes once for B, sterilization: 70% alcohol-pickled 30s.Whether the water after sample embathes is for the last time contained in the small beaker of sterilizing, at flat lining out in contrast, thorough with the sterilization of check sample, after some days, generates if any bacterium colony, shows that sample surfaces sterilization is not thorough, needs to continue to adjust sterilization method
[12-14];
The selection of C, inoculation position: phyllade sprig: divide the phyllade sprig of three parts in upper, middle and lower of getting plant, each phyllade sprig is divided into again a tip, branch middle part and 3 processing of branch base portion; Branch: upper, middle and lower is divided into 3 positions, wherein the 3rd Wei Zhijing junction, position; Root: be divided into 2 parts of the tip of a root and foundation;
D, inoculation: the explant after sterilization is cut with vaccinating lancet, be laid in media surface, observe colony growth situation cultivate 5-7 days in 28 ℃ of constant incubators after.Some bacterial strain breedings rapidly, can first be carried out separation and purification by the bacterial strain of its generation; The bacterial strain of poor growth and comparatively small amt can extend observing time, until the stable rear purifying of its growth.
Solid culture: use improvement Martin solid medium, formula for peptone 5.0g/L, yeast extract powder 2.0g/L, glucose 20.0g/L, dipotassium hydrogen phosphate 1.0g/L, magnesium sulfate 0.5g/L, agar 14.0g/L, other is sterilized water, pH value 6.4 ± 0.2, culture temperature is 28 ℃, training method is dull and stereotyped cultivation, and incubation time is 48h;
E, different types of endogenetic fungus access plate culture medium that separation is bred carry out purifying, obtain the above-mentioned Aspergillus sp. function stem of single culture after 2-3 point connects purifying, switching;
It is on plating medium, and bacterium colony is circular white down, and outermost layer is water profit transparence; Back is oyster white, and the speed of growth is extremely slow.Conidiophore is upright, and simple, top is spherical, and top raw stigma; Conidium (stalk spore) monospore is spherical; Be accredited as Aspergillus (Aspergillus sp.) with reference to fungi identification handbook, be kept at China Committee for Culture Collection of Microorganisms's common micro-organisms center, preserving number is CGMCC No.6311.
The preparation method of above-mentioned a kind of Aspergillus strain application bacterium liquid that can promote Horsetail Beefwood root growth, by above-mentioned bacterial strain access liquid nutrient medium, shaking table shaking culture, culture temperature is 28 ℃, incubation time 48～72h, utilize blood counting chamber to calculate bacterial concentration, bacterium liquid is diluted to 1.0-9.0 × 10 with ultrapure water
6it is for subsequent use that cfu/ml is finished product; Liquid nutrient medium: be improvement Martin substratum, wherein peptone 5.0g/L, yeast extract powder 2.0g/L, glucose 20.0g/L, dipotassium hydrogen phosphate 1.0g/L, magnesium sulfate 0.5g/L, other is sterilized water, pH value 6.4 ± 0.2.
The application method of above-mentioned a kind of Aspergillus strain application bacterium liquid that can promote Horsetail Beefwood root growth, is the bacterium liquid of this bacterial strain of application, and transplanted seedling tree dips in root and waters root for forest land early stage.
The application method of above-mentioned a kind of Aspergillus strain application bacterium liquid that can promote Horsetail Beefwood root growth is with 9.0 × 10
5cfu/ml bacterium liquid, waters in the rhizosphere of every strain Horsetail Beefwood in forest land by every strain 100ml.
The application method of above-mentioned a kind of Aspergillus strain application bacterium liquid that can promote Horsetail Beefwood root growth is the bacterium liquid 5.0 × 10 of this bacterial strain of application
5cfu/ml dips in seedling root 10min before Rooted Cuttings is planted.
The bacterial strain the present invention relates to separation and purification from Horsetail Beefwood plant obtains, aimed strain be aspergillus (
aspergillus sp.) Horsetail Beefwood Flora of Rhizosphere Fungi 63, registered preservation at China Committee for Culture Collection of Microorganisms's common micro-organisms center on June 28th, 2012, be called for short CGMCC, address is No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, and deposit number is CGMCC No. 6311.
Horsetail Beefwood Flora of Rhizosphere Fungi 63 is inoculated in to Horsetail Beefwood Rooted Cuttings, comprehensively passes judgment on according to the indices of root growth, further confirm that it has promoter action to root growth, search out promoting that the useful function endogenetic fungus of root growth can be applicable to produce.
No. 63 processing plant of bacterial strain contrast seedling root total surface area has increased by 71.97%, root system overall length has increased by 47.90%, under the level of≤0.25mm footpath, contrast increases by 37.98%, under the level of 0.25 ~ 0.5mm footpath, contrast significantly increases by 59.59%, under 0.5 ~ 0.75 mm, 0.75 ~ 1 mm and >=1 mm root system footpath level, long being all significantly higher than of the root of No. 63 nursery stocks of bacterial strain contrasts nursery stock.Therefore the long amplification of root that, the Horsetail Beefwood nursery stock under No. 63 processing of bacterial strain contrasts under the level of all footpaths of CK reaches maximum.Show that it has significant effect for the root growth that promotes plant.
Embodiment
1, the application in water planting breeding
Get the above-mentioned endogenetic fungus application bacterium liquid preparing, make 5.0 × 10
5cfu/ml bacterium liquid dips in root 10min before Rooted Cuttings is planted.Can improve and plant seedlings surviving rate more than 10%.
2, rhizosphere soil waters and executes application
Test materials is No. one, Huian Rooted Cuttings, and nursery stock is potted plant in forest ecology institute of University Of Agriculture and Forestry In Fujian field test ground in July, 2011.
After Rooted Cuttings root system length is neat, is moved into the soil that gold zone loam and the husky ratio with 3:1 are mixed, and be sub-packed in approximately 500 diameter 22cm, in the flowerpot of high 15cm.Every basin is put into the 3.36KG mixed soil of equal mass, in order to guarantee that later stage nursery stock connects the accuracy of bacterium, in every basin soil, splashes into 10 ~ 15 formaldehyde (formalin) thimerosal, and with plastics film capping basin mouth, disinfecting time is 24h.Treat that in soil, formaldehyde infiltration sterilization completely, is removed plastics film, by remaining formaldehyde evaporation, in order to avoid affect the transplanting success of seedling.Through the restorative growth of month, applying bacterial strain concentration at Horsetail Beefwood rhizosphere was 9.0 × 10
5the bacterium liquid 100ml of cfu/ml, repeats 3 times, processes as blank with distilled water solution.
The preparation of bacterium liquid: by Horsetail Beefwood Flora of Rhizosphere Fungi 63(bacterial strain 63) access liquid nutrient medium, substratum is improvement Martin substratum, every L is containing peptone 5.0g, yeast extract powder 2.0g, glucose 20.0g, dipotassium hydrogen phosphate 1.0g, magnesium sulfate 0.5g, and other is sterilized water, pH value 6.4 ± 0.2.Through the cultivation of 24h, utilize blood counting chamber to calculate bacterial concentration, bacterium liquid is diluted to 1.0 × 10 with ultrapure water
6cfu/ml to 9.0 × 10
6cfu/ml is for subsequent use.Inoculate the mensuration of carrying out the indexs such as root growth after latter 30 days, detection method and result are as follows:
2.1 bacterial strains water and execute rear wooden snippings is growth parameter(s) analysis
Inoculate the effect to Horsetail Beefwood seedling root in order to study endogenetic fungus, test has the flowerpot of Horsetail Beefwood nursery stock to topple over cultivation, take out the whole seedlings with soil and grit, after repeatedly rinsing with clear water, dry, cut underground part, be respectively charged in Envelope bag for subsequent use, 15 strain bacterial strain processing under point S and P inoculation method, 3 seedlings are adopted in each processing.Application Epson (10000XL, Epson Inc., Beijing) root scanner, deposits complete root system image scanning in computer, application (WinRHIZO
tM2009) professional Root morphology and analytical system of structure software (Regent Instruments Canada Inc.) carry out quantitative analysis to root system total surface area, root system overall length and root system footpath level (root average diameter classification)
[15], get the mean value of 3 repetition values as the Root Characteristics value of this processing nursery stock.Under same inoculation method, connect vaccine wood and the relatively employing ANOVA that do not connect between vaccine wood analyzes, different strains adopts Duncan multiple comparisons between processing, and represents with labelled notation.
interpretation of result
the impact of 3.1 different treatment on seedling root total surface area
The exchange of nutritive substance between the affects plant root of root system surface-area and soil; itself and plant moisture receptivity are closely connected; can roughly reflect the utilize present situation of nursery stock to edatope; if root system surface-area is larger; Root Distribution is just wider; plant can absorb the nutrient in soil better; simultaneously; the size of root system and the depth are also determining the development degree of root system of plant; closely related with wind resistance, drought tolerance and the vitality of plant, especially coastal or the soil conservation plant of checking winds and fixing drifting sand is also had to important impact
[16-19].
By one-way analysis of variance and Duncan multiple comparisons, the root system total surface area between different strains processing has significant difference (S:sig=0.000; P:sig=0.000), the root system total area of different treatment, bacterial strain reaches significant difference No. 63 compared with control treatment, and contrasting seedling root total surface area has increased by 71.97%.
the impact of different treatment on seedling root overall length
The same important parameter as describing Root Characteristics of total length of plant root, the size of its value is also determining the size of root system surface-area and the depth that root system spreads all over.
One-way analysis of variance shows, the root system overall length of the each bacterial strain of different inoculation methods between processing all there were significant differences (S:sig=0.000; P:sig=0.000), No. 63, bacterial strain reaches significant difference with contrasting, and contrasting seedling root overall length has increased by 47.90%.
the impact of different treatment on seedling root footpath level
Test is carried out careful statistics by stepped diameter (the minimum 0.01mm of unit that differentiates) to the root system of every strain plant by WinRHIZO root system analysis software, due in this test, Casuarina equisetifolia Seedling is clone water planting nursery stock, ≤ 0.25,0.25 ~ 0.5,0.5 ~ 0.75,0.75 ~ 1, >=1 (unit: mm) its root system is the radicula of diameter < 2mm, therefore the root system of Horsetail Beefwood water planting seedling is divided into level interval, five footpaths:.
As everyone knows, Casuarina equisetifolia Growth is in areas such as the abundant subtropics of annual precipitation, seeds itself have certain waterlogging-resistant property, Osundina studies discovery, and in waterflooding situation, Horsetail Beefwood can be exposed to the adventive root quantity on the water surface by increase, thereby the anoxic conditions of root system while alleviating Horsetail Beefwood waterlogging, therefore, the root system of plant, particularly the radicula of diameter < 2mm has responsive response to the situation of edatope resource of living in
[20-22]; By the size of plant root total surface area and root system overall length can reflect intuitively the biomass situation of foot end and the photosynthate that consumes number, for the further impact of research inoculation endogenetic fungus on Casuarina equisetifolia Seedling radicula configuration and functional character, this test has been added up under Different Diameter level and has respectively been connect the growth pattern that bacterium processing nursery stock contrasts by root system analysis software, tentatively to find the rule that connects bacterium and the each diameter yield variation of seedling root.Different inoculation method different strains are processed the eigenwert of lower seedling root Different Diameter level.
Data by table 1 can find out, under bacterial classification 63 is processed, under the level of≤0.25mm footpath, bacterial strain is processed the long amplification maximum of root of nursery stocks for No. 63, and contrast increases by 37.98%; Under the level of 0.25 ~ 0.5mm footpath, the long contrast of root that bacterial strain is processed nursery stock for No. 63 significantly increases by 59.59%; Under 0.5 ~ 0.75 mm, 0.75 ~ 1 mm and >=1 mm root system footpath level.Therefore the long amplification of root that, the Horsetail Beefwood nursery stock under No. 63 processing contrasts under the level of all footpaths of CK reaches maximum.
The impact of table 1 different treatment on the each footpath of Horsetail Beefwood seedling root level root length
* in the same columns value in table, there are different lowercase persons to represent significant difference (P < 0.05).
The present invention finds that a strain can promote the endogenetic fungus of Horsetail Beefwood root growth, and this strain Horsetail Beefwood endogenetic fungal bacterial strain is adopted and dips in root or bacterium liquid and water the method for executing and be inoculated in Casuarina equisetifolia Seedling.By inoculating the chlorophyll fluorescence test of rear plant, show that the root growth ability of bacterial strain is all largely higher than contrast, show that it has very large function for the root growth usefulness that promotes plant, thereby further confirm that obtaining this strain endogenetic fungus can promote Horsetail Beefwood root growth.
Reference
[1] Guo Liangdong. Recent Research Advance in Endophytic Fungi [J]. fungus system, 2001,20 (1): 148 ~ 152.
[2] in Hanshou, Ji Yanling, Weng Zhonghe etc. the biological characteristics in seedling stage [J] of grass endogenetic fungus research 10--endogenetic fungus symbiote. Practaculture Science, 2009,26 (10): 150-154.
[3]Lu?H,Zou?W?X,Meng?J?C,Hu?J,Tan?R?X.New?bioactive?metabolites?produced?by?Colletotrichum?sp.,an?endophytic?fungus?in?Artemisia?annua?[J].Plant?Sci,2000,151:67-73.
[4]Reis?V?M,Baldani?V?L?D,Dobereiner?J.Biological?dinitrogen?fixation?in?Cramineae?and?palm?trees[J].?Critical?Rev?Plant?Sci,2000,19:227-247.
[5]Stone?J?K,?Bacon?C?E,?White?J?F?Jr.?An?overview?of?endophytic?microbes:?endophytism?defined?Microbial?Endophytes[J]?.?New?York:Marcel?Dekker，2000;3-29.
[6] Feng Yongjun, Song Wei. endophytic bacterium [J]. Nature Journal, 2001,23 (5): 249 ~ 252.
[7] Liu Yunxia. the research of endophytic bacterium and application [J]. plant protection, 1994,20 (5): 30 ~ 32.
[8] Yang Hailian, Sun Xiaolu, Song Wei. the research [J] of endophytic bacterium. microbiology circular, 1998,25 (4): 224 ~ 227.
[9] Lin Yanqing, Hong Wei. the statistical study [J] of Chinese Wood Chinese ephedra Research Literature. protection forest science and technology, 2011, (01): 46-50.
[10] Zhang Yong, Zhong Chonglu, Chen Yu, the progress [J] of et al. Casuarina Symbiotic Microbe. Annual, 2006,22 (001): 70-75.
[11]Guérin?P.?Sur?la?présence?d’un?champignon?dans?l’ivraie[J].?Journal?de?Botanique,?1898,?12:?230-238.
[12] Cui Lin, Sun Zhen, Sun Fu, the separation of et al. potato endogenetic bacteria and the Screening and Identification [J] of ring rot Antagonistic Fungi. Plant Pathology, 2003,33 (004): 353-358.
[13] He Hong, Cai Xueqing, Hong Yongcong, the separation of et al. capsicum endogenetic bacteria and the screening [J] of Antagonistic Fungi. Chinese biological control, 2002,18 (4): 171-175.
[14] Yuan Jun, Sun Fu, Tian Hongxian, et al. prevents and treats the Isolation and screening [J] of the useful endogenetic bacteria of bacterial ring rot o potato. microorganism journal, 2002,42 (3): 270-274.
[15]L?H?M,?W?L,?F?K.?Estimating?length,average?diameter?and?surface?area?of?roots?using?two?different?image?analyses?systems[J].?Plant?Soil,?2004,?260:?111-120.
[16] Zhao Zhong, Cheng Xiangrong, Xue Wenpeng, the difference [J] of different in moisture ecotope, et al. loess plateau locust tree radicula vertical distribution. forest-science, 2006,42 (11): 1-7.
[17]Berntson?G,?Wayne?P.?Characterizing?the?size?dependence?of?resource?acquisition?within?crowded?plant?populations[J].?Ecology,?2000,?81(4):?1072-1085.
[18] Yang Guohu, Li Jiansheng, Luo Xiangning, the research [J] of Estimating Leaf Area In Maize variation and ground dry material accumulation and distribution under et al. drought condition. Journal of Northwest Sci Tech University of Agriculture and Forestry: natural science edition, 2005,33 (5): 27-32.
[19] Han Xiying, Song Fengbin. the impact [J] of drought stress on Maize Roots and rhizosphere nutrient. soil conservation journal, 2006,20 (3): 170-172.
[20] Chen Yan, kingdom is bright, and week is hard. Stress Resistance of Casuarina equisetifolia progress [J]. BULLETIN OF BOTANY Vol., 2005, (6): 746-752.
[21]Osundina?M.?Nodulation?and?growth?of?mycorrhizal?Casuarina?equisetifolia?JR?and?G.?First?in?response?to?flooding[J].?Biology?and?fertility?of?soils,?1997,?26(2):?95-99.
[22]Hendrick?R?L,?Pregitzer?K?S.?The?dynamics?of?fine?root?length,?biomass,?and?nitrogen?content?in?two?northern?hardwood?ecosystems[J].?Canadian?Journal?of?Forest?Research,?1993,?23(12):?2507-2520.
Claims (2)
1. a strain can promote the Aspergillus strain of Horsetail Beefwood root growth, for aspergillus (
aspergillus sp.) Horsetail Beefwood Flora of Rhizosphere Fungi 63, registered preservation at China Committee for Culture Collection of Microorganisms's common micro-organisms center on June 28th, 2012, deposit number is CGMCC No. 6311.
2. comprise an application bacterium liquid for aspergillus as claimed in claim 1, it is characterized in that: the preparation method of described application bacterium liquid, be by described aspergillus (
aspergillus sp.) Horsetail Beefwood Flora of Rhizosphere Fungi 63 accesses liquid nutrient medium, shaking table shaking culture, culture temperature is 28 ℃, incubation time 48～72h utilizes blood counting chamber to calculate bacterial concentration, and bacterium liquid is diluted to 1.0-9.0 × 10 with ultrapure water
6cfu/ml, for subsequent use; Liquid nutrient medium is improvement Martin substratum, wherein peptone 5.0g/L, yeast extract powder 2.0g/L, glucose 20.0g/L, dipotassium hydrogen phosphate 1.0g/L, magnesium sulfate 0.5g/L, other is sterilized water, pH value 6.4 ± 0.2.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310068757.XA CN103194396B (en) | 2013-03-05 | 2013-03-05 | Aspergillus strain capable of promoting root growth of Casuarina |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310068757.XA CN103194396B (en) | 2013-03-05 | 2013-03-05 | Aspergillus strain capable of promoting root growth of Casuarina |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103194396A CN103194396A (en) | 2013-07-10 |
| CN103194396B true CN103194396B (en) | 2014-07-09 |
Family
ID=48717215
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310068757.XA Expired - Fee Related CN103194396B (en) | 2013-03-05 | 2013-03-05 | Aspergillus strain capable of promoting root growth of Casuarina |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103194396B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110129210B (en) * | 2019-05-21 | 2020-05-15 | 天津农学院 | Aspergillus aculeatus strain and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102732435A (en) * | 2012-07-06 | 2012-10-17 | 南京林业大学 | Masson pine rhizosphere solubilizing phosphorus fungus aspergillus awamori and application thereof |
-
2013
- 2013-03-05 CN CN201310068757.XA patent/CN103194396B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102732435A (en) * | 2012-07-06 | 2012-10-17 | 南京林业大学 | Masson pine rhizosphere solubilizing phosphorus fungus aspergillus awamori and application thereof |
Non-Patent Citations (8)
| Title |
|---|
| Anju Keshawat et al.Microflora Associated With The Seeds Of Casuarina Equisetifolia.《Asian Journal of Biochemical and Pharmaceutical Research》.2012,第2卷(第1期),101-104. |
| Microflora Associated With The Seeds Of Casuarina Equisetifolia;Anju Keshawat et al;《Asian Journal of Biochemical and Pharmaceutical Research》;20121231;第2卷(第1期);101-104 * |
| 三种丛枝菌根真菌对辣椒根系生长的影响及效应分析;江盼盼 等;《中国蔬菜》;20101231;第6卷;58-62 * |
| 日本曲霉产生的黑麦酮酸F对玉米的化感作用;曾任森 等;《应用生态学报》;20040131;第15卷(第1期);145-148 * |
| 曾任森 等.日本曲霉产生的黑麦酮酸F对玉米的化感作用.《应用生态学报》.2004,第15卷(第1期),145-148. |
| 江盼盼 等.三种丛枝菌根真菌对辣椒根系生长的影响及效应分析.《中国蔬菜》.2010,第6卷58-62. |
| 适应玉米生产的溶磷真菌筛选及其应用;龚月波 等;《微生物学报》;20101204;第50卷(第12期);1619-1625 * |
| 龚月波 等.适应玉米生产的溶磷真菌筛选及其应用.《微生物学报》.2010,第50卷(第12期),1619-1625. |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103194396A (en) | 2013-07-10 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104805019B (en) | One plant of endogenetic fungus that can promote aleurite montana Nutrient Absorption | |
| CN104004666B (en) | One strain can promote the endogenetic fungus of Growth of Chinese Fir | |
| CN103173359B (en) | Endophytic fungus promoting casuarina equisetifolia root system growth effect | |
| CN103173364B (en) | Endophytic fungus promoting casuarina equisetifolia biomass growth | |
| CN104762219A (en) | Endophytic fungus for promoting biomass growth of aleurites montana in low-phosphorus environment | |
| CN106929433B (en) | Phosphate solubilizing penicillium and application thereof | |
| CN104004667A (en) | Endophytic fungus capable of accelerating phosphorus absorption of cedar | |
| CN104004664B (en) | One strain can improve the photosynthetic endogenetic fungus of China fir | |
| CN103173361B (en) | Endophytic fungus promoting casuarina equisetifolia nutrient element absorption | |
| CN103194396B (en) | Aspergillus strain capable of promoting root growth of Casuarina | |
| CN103173360B (en) | Endophytic fungus increasing casuarina equisetifolia chlorophyll content | |
| CN103173362B (en) | Endophytic fungus promoting casuarina equisetifolia photosynthesis | |
| CN104789481A (en) | Endophytic fungus for promoting growth and photosynthesis enhancement of aleurites montana in low-phosphorus environment | |
| CN104004665B (en) | One strain can alleviate the China fir endogenetic fungus of P deficiency | |
| CN106010984B (en) | One plant of endogenetic fungus for promoting acacia confusa biomass to increase under low-phosphorous environment | |
| CN101993827B (en) | Functional endophytic fungus for prompting photosynthesis of eucalypt and application thereof | |
| CN103173363B (en) | Phyllosticta strain promoting casuarina equisetifolia photosynthesis | |
| CN115948254B (en) | Trichoderma viride 192-45 and application thereof | |
| CN112779168B (en) | Aureobasidium-free cystic strain JX2 and application thereof | |
| CN105969672A (en) | Mixed endophytic fungi capable of promoting growth of heights and ground diameters of Taiwan acacia seedlings | |
| CN106085872A (en) | A kind of mixing endogenetic fungus that can promote acacia confusa Nutrient Absorption | |
| CN104818219A (en) | Endophytic fungus for promoting root growth of aleurites montana in low-phosphorous environment | |
| CN105861335B (en) | The endogenetic fungus of one plant of promotion acacia confusa Nutrient Absorption under low-phosphorous environment | |
| CN104774771B (en) | One plant promotes the photosynthetic endogenetic fungus of aleurite montana under low-phosphorous environment | |
| CN106085873B (en) | The mixing endogenetic fungus that acacia confusa P elements can be promoted to absorb under low-phosphorous environment |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140709 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |