CN105969672A - Mixed endophytic fungi capable of promoting growth of heights and ground diameters of Taiwan acacia seedlings - Google Patents

Mixed endophytic fungi capable of promoting growth of heights and ground diameters of Taiwan acacia seedlings Download PDF

Info

Publication number
CN105969672A
CN105969672A CN201610452322.9A CN201610452322A CN105969672A CN 105969672 A CN105969672 A CN 105969672A CN 201610452322 A CN201610452322 A CN 201610452322A CN 105969672 A CN105969672 A CN 105969672A
Authority
CN
China
Prior art keywords
bacterial strain
fusarium
trichoderma
endogenetic fungus
seedling
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN201610452322.9A
Other languages
Chinese (zh)
Other versions
CN105969672B (en
Inventor
周艳芬
林晗
陈灿
林宇
洪滔
吴承祯
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fujian Agriculture and Forestry University
Original Assignee
Fujian Agriculture and Forestry University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fujian Agriculture and Forestry University filed Critical Fujian Agriculture and Forestry University
Priority to CN201610452322.9A priority Critical patent/CN105969672B/en
Publication of CN105969672A publication Critical patent/CN105969672A/en
Application granted granted Critical
Publication of CN105969672B publication Critical patent/CN105969672B/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/885—Trichoderma
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00—Botany in general
    • A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/145—Fungal isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/77—Fusarium

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • General Engineering & Computer Science (AREA)
  • Environmental Sciences (AREA)
  • Biochemistry (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Ecology (AREA)
  • Forests & Forestry (AREA)
  • Dentistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention discloses mixed endophytic fungi capable of promoting growth of heights and ground diameters of Taiwan acacia seedlings. The mixed endophytic fungi consists of Trichoderma sp. strains A and Fusarium sp. strains E, and is registered and stored by the general microbiology centre of the microbial preservation management committee in China; the storage No. of the strains A is CGMCC No. 11911, and the storage No. of the strains E is CGMCC No. 11909. The strains of the mixed endophytic fungi are separated and purified from the Taiwan acacia seedlings; the mixed endophytic fungi is prepared into an application bacterial solution, so as to achieve the effect of promoting growth of the heights and the ground diameters of the Taiwan acacia seedlings, and promote the growth of plants.

Description

A kind of mixing endogenetic fungus that acacia confusa height of seedling and footpath, ground can be promoted to increase
Technical field
The present invention relates to a kind of mixing endogenetic fungus that acacia confusa height of seedling and footpath, ground can be promoted to increase and application thereof.
Background technology
The research of endophyte of plant started from for 19 end of the centurys, and Vogl isolates the first strain endophyte from rye grass Lolium temulentum L. seed.But really start the endophyte in numerous studies plant and originate in the eighties in last century, mainly conduct a research in the vegetation of Temperate Region in China, subtropical zone and torrid areas.
The distribution of plant endogenesis epiphyte is wide, kind is many, and forefathers' research shows, can isolated endogenetic fungus in most plants, it can be seen that endogenetic fungus is prevalent in plant.At least in more than 80 gramineae farm crop belonging to kind more than 290, it is found that endophyte in worldwide.The endo-mycorrhiza separated from plant at present can be divided into according to its unique function having: the plant growth-promoting bacterias such as nitrogen-fixing bacteria, solid potassium bacterium, solid phosphorus bacterium, has the biocontrol microorganisms of disease and insect resistance and has the promotion plant resistance bacterium to the repair ability of poor environment.
Through consulting literatures material, the influence research grown acacia confusa about endogenetic fungus at present is also blank.
Summary of the invention
It is an object of the invention to provide a kind of mixing endogenetic fungus that acacia confusa height of seedling and footpath, ground can be promoted to increase, its bacterial strain uses therefor is isolated and purified from acacia confusa plant to be obtained, and the application bacteria liquid has that it is made promotes the effect that acacia confusa height of seedling and footpath, ground increase.
For achieving the above object, the present invention adopts the following technical scheme that
A kind of can promote acacia confusa height of seedling and ground footpath increase mixing endogenetic fungus, its by acacia confusa endogenetic fungus trichoderma (Trichoderma sp.) A bacterial strain and Fusarium (Fusarium sp.) E bacterial strain composition；Described trichoderma (Trichoderma sp.) A bacterial strain and Fusarium (Fusarium sp.) E bacterial strain on January 27th, 2016 China Committee for Culture Collection of Microorganisms's common micro-organisms center registration preservation (address is Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3), wherein, the preserving number of A bacterial strain is CGMCC No. 11911, and the preserving number of E bacterial strain is CGMCC No. 11909.
The separation of acacia confusa endogenetic fungus used, purge process include:
A, material: by collecting the root of different year, branch stem, leaf are divided into three parts, clean up with tap water, are dispensed in valve bag, in 4 DEG C of Refrigerator stores；
B, sterilization: sodium hypochlorite rinsing (root 10min, stem 5min, leaf 2min) → aseptic water washing of 75% ethanol rinsing 30s → aseptic water washing 3-4 time → 15% 4-5 time；Water after being embathed for the last time by sample is contained in the small beaker of sterilizing, and at flat lining out as comparison, the sterilization with sample survey is the most thorough, generates if any bacterium colony behind some skies, then show that sample surfaces sterilization is not thorough, need to continue to adjust sterilization method；
C, the selection of inoculation position: blade: leaf tip, leaf central part and leaf base 3 process；Branch stem: upper, middle and lower is divided into 3 positions, and wherein the 3rd position is branch stem junction；Root: be divided into the tip of a root and 2 parts of foundation；
D, inoculation: cut by the outer implant vaccinating lancet after sterilization, be laid in media surface, observe colony growth situation in 28 DEG C of constant incubators after cultivating 5-7 days；Rapidly, the bacterial strain that can be first produced carries out isolated and purified the breeding of some bacterial strains；The bacterial strain of poor growth and negligible amounts can extend observing time, until purification after its growth is stable；
Solid culture: use improvement Martin's solid medium, formula is peptone 5.0g/L, yeast extract powder 2.0g/L, glucose 20.0g/L, dipotassium hydrogen phosphate 1.0g/L, magnesium sulfate 0.5g/L, agar 14.0g/L, other be sterilized water, pH value 6.4 ± 0.2, cultivation temperature is 28 DEG C, training method is that flat board is cultivated, and incubation time is 48h；
E, different types of endogenetic fungus separation bred access plating medium and are purified, and respectively obtain the above-mentioned of single culture after 2-3 point connects purification, switchingTrichoderma sp.WithFusarium sp.Function stem.
Gained can promote that the mixing endogenetic fungus that acacia confusa height of seedling and footpath, ground increase can be prepared as applying bacterium solution, and the rhizosphere soil for acacia confusa nursery stock waters grants nursery stock direct inoculation.
The preparation method of described application bacterium solution be respectively by trichoderma (Trichoderma sp.) A bacterial strain and Fusarium (Fusarium sp.) E bacterial strain access fluid medium, shaking table shaken cultivation, cultivation temperature is 28 DEG C, incubation time 48 ~ 72h, utilizes blood counting chamber to calculate bacterial concentration, then bacterium solution ultra-pure water is diluted to 5.5 × 106Cfu/mL, then two kinds of bacterium solution are mixed to prepare by 1:1 by volume；Described fluid medium for improvement Martin's culture medium, wherein peptone 5.0g/L, yeast extract powder 2.0g/L, glucose 20.0g/L, dipotassium hydrogen phosphate 1.0g/L, magnesium sulfate 0.5g/L, other be sterilized water, pH value 6.4 ± 0.2.
The using method of this application bacterium solution is rhizosphere gained application bacterium solution being watered by every strain 100mL and imposing on acacia confusa.
Bacterial strain uses therefor of the present invention is isolated and purified from acacia confusa plant to be obtained, and it has the effect promoting that acacia confusa height of seedling and footpath, ground increase, thus promotes plant strain growth.
The application bacterium solution prepared by this mixing endogenetic fungus is inoculated in acacia confusa seedling by watering the method executed, measure inoculation 4 months, 6 months time acacia confusa height of seedling, footpath.Result shows, the height of seedling rate of increase of plant and adjoining tree that acacia confusa endogenetic fungus AE processes, footpath rate of increase significant difference, the height of seedling rate of increase that strains A E processes is 2.3 times that footpath, ground rate of increase is comparison that 1.5 times of strains A E of comparison process, it was demonstrated that this mixing endogenetic fungus AE to acacia confusa height of seedling, the growth in footpath there is obvious facilitation.
Detailed description of the invention
A kind of can promote acacia confusa height of seedling and ground footpath increase mixing endogenetic fungus, its by acacia confusa endogenetic fungus trichoderma (Trichoderma sp.) A bacterial strain and Fusarium (Fusarium sp.) E bacterial strain composition；Described trichoderma (Trichoderma sp.) A bacterial strain and Fusarium (Fusarium sp.) E bacterial strain on January 27th, 2016 China Committee for Culture Collection of Microorganisms's common micro-organisms center register preservation, wherein, the preserving number of A bacterial strain is CGMCC No. 11911, and the preserving number of E bacterial strain is CGMCC No. 11909.
The described mixing endogenetic fungus that can promote that acacia confusa height of seedling and footpath, ground increase can be prepared as applying bacterium solution, and the rhizosphere soil for acacia confusa nursery stock waters grants nursery stock direct inoculation.
The preparation method of described application bacterium solution be respectively by trichoderma (Trichoderma sp.) A bacterial strain and Fusarium (Fusarium sp.) E bacterial strain access fluid medium, shaking table shaken cultivation, cultivation temperature is 28 DEG C, incubation time 48 ~ 72h, utilizes blood counting chamber to calculate bacterial concentration, then bacterium solution ultra-pure water is diluted to 5.5 × 106Cfu/mL, then two kinds of bacterium solution are mixed to prepare by 1:1 by volume；Described fluid medium for improvement Martin's culture medium, wherein peptone 5.0g/L, yeast extract powder 2.0g/L, glucose 20.0g/L, dipotassium hydrogen phosphate 1.0g/L, magnesium sulfate 0.5g/L, other be sterilized water, pH value 6.4 ± 0.2.
In order to make content of the present invention easily facilitate understanding, below in conjunction with detailed description of the invention, technical solutions according to the invention are described further, but the present invention is not limited only to this.
Rhizosphere soil waters grants the application of nursery stock direct inoculation
This test uses earth culture pot experiment, the acacia confusa one year seedling that test material therefor provides for Municipal Forestry Bureau of Zhangzhou City of Fujian Province.Select the consistent acacia confusa seedling planting of growing way in diameter 15cm, high 10cm plastic tub in.Soil used is strictly through the yellow soil of fumigation.Weighing through mixing, 3kg soil put into by every basin.After the restorative growth of month, continuous three days apply 100mL concentration in acacia confusa rhizosphere is 5.5 × 106Bacterium solution AE of cfu/mL, separately waters with distilled water solution and applies as blank CK.
Prepared by bacterium solution: strains tested is respectively connected to the fluid medium of 40mL, culture medium for improvement Martin's culture medium, wherein peptone 5.0g/L, yeast extract powder 2.0g/L, glucose 20.0g/L, dipotassium hydrogen phosphate 1.0g/L, magnesium sulfate 0.5g/L, other be sterilized water, pH value 6.4 ± 0.2；Through the cultivation of 72h in constant-temperature shaking incubator, press decimal dilution method with physiological saline solution and bacterium solution is diluted, utilize blood counting chamber to calculate bacterial concentration, then bacterium solution ultra-pure water is diluted to 5.5 × 106Cfu/mL, then two kinds of bacterium solution are mixed to prepare by 1:1 by volume.
In inoculate 4 months, 6 months time, use steel ruler measure height of seedling, electronic digital indicator measure ground footpath, the results are shown in Table 1.
Table 1 different disposal is on acacia confusa seedling footpath, ground and the impact of height of seedling
As shown in table 1, the seedling height of seedling rate of increase of inoculating strain AE is 47.432%, and comparison is only 26.438%, can be obtained by variance analysis, acacia confusa seedling height of seedling rate of increase after strains A E processes reaches pole significant level (sig=0.000, significant level is 0.01) compared with matched group.Footpath, the seedling ground rate of increase of inoculating strain AE is 35.466%, and comparison is only 15.190%, reaches pole significant level (sig=0.001, significant level is 0.01) with matched group diversity.
As can be seen here, the mixing endogenetic fungus that the present invention provides can remarkably promote acacia confusa height of seedling and the growth in footpath, ground, thus promotes plant strain growth.
The foregoing is only presently preferred embodiments of the present invention, all impartial changes done according to scope of the present invention patent and modification, all should belong to the covering scope of the present invention.

Claims (3)

1. one kind can promote acacia confusa height of seedling and ground footpath increase mixing endogenetic fungus, it is characterised in that: this mixing endogenetic fungus by acacia confusa endogenetic fungus trichoderma (Trichoderma sp.) A bacterial strain and Fusarium (Fusarium sp.) E bacterial strain composition；Described trichoderma (Trichoderma sp.) A bacterial strain and Fusarium (Fusarium sp.) E bacterial strain on January 27th, 2016 China Committee for Culture Collection of Microorganisms's common micro-organisms center register preservation, wherein, the preserving number of A bacterial strain is CGMCC No. 11911, and the preserving number of E bacterial strain is CGMCC No. 11909.
2. the application of the mixing endogenetic fungus that acacia confusa height of seedling and footpath, ground can be promoted to increase as claimed in claim 1, it is characterised in that: being prepared as described mixing endogenetic fungus applying bacterium solution, the rhizosphere soil for acacia confusa nursery stock waters grants nursery stock direct inoculation.
Can promote the most according to claim 2 acacia confusa height of seedling and ground footpath increase mixing endogenetic fungus application, it is characterised in that: the preparation method of described application bacterium solution be respectively by trichoderma (Trichoderma sp.) A bacterial strain and Fusarium (Fusarium sp.) E bacterial strain access fluid medium, shaking table shaken cultivation, cultivation temperature is 28 DEG C, incubation time 48 ~ 72h, utilizes blood counting chamber to calculate bacterial concentration, then bacterium solution ultra-pure water is diluted to 5.5 × 106Cfu/mL, then two kinds of bacterium solution are mixed to prepare by 1:1 by volume；
Described fluid medium for improvement Martin's culture medium, wherein peptone 5.0g/L, yeast extract powder 2.0g/L, glucose 20.0g/L, dipotassium hydrogen phosphate 1.0g/L, magnesium sulfate 0.5g/L, other be sterilized water, pH value 6.4 ± 0.2.
CN201610452322.9A 2016-06-22 2016-06-22 A kind of mixing endogenetic fungus that acacia confusa height of seedling and ground diameter can be promoted to increase Expired - Fee Related CN105969672B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201610452322.9A CN105969672B (en) 2016-06-22 2016-06-22 A kind of mixing endogenetic fungus that acacia confusa height of seedling and ground diameter can be promoted to increase

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201610452322.9A CN105969672B (en) 2016-06-22 2016-06-22 A kind of mixing endogenetic fungus that acacia confusa height of seedling and ground diameter can be promoted to increase

Publications (2)

Publication Number Publication Date
CN105969672A true CN105969672A (en) 2016-09-28
CN105969672B CN105969672B (en) 2019-03-12

Family

ID=57021583

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201610452322.9A Expired - Fee Related CN105969672B (en) 2016-06-22 2016-06-22 A kind of mixing endogenetic fungus that acacia confusa height of seedling and ground diameter can be promoted to increase

Country Status (1)

Country Link
CN (1) CN105969672B (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106754426A (en) * 2017-01-17 2017-05-31 中国科学院烟台海岸带研究所 A kind of trichoderma asperellum and its application

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104762219A (en) * 2015-04-14 2015-07-08 福建农林大学 Endophytic fungus for promoting biomass growth of aleurites montana in low-phosphorus environment

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104762219A (en) * 2015-04-14 2015-07-08 福建农林大学 Endophytic fungus for promoting biomass growth of aleurites montana in low-phosphorus environment

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
S. PIRIYAPRIN等: "Identification and Characterization of Halophilic, Endophytic Fungi from Acacia ampliceps", 《THAI JOURNAL OF AGRICULTURAL SCIENCE》 *
吕成群等: "相思树种根瘤菌的生物学特性", 《微生物学通报》 *
石前: "石灰岩地区台湾相思根瘤内生细菌的初步研究", 《万方数据知识服务平台》 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106754426A (en) * 2017-01-17 2017-05-31 中国科学院烟台海岸带研究所 A kind of trichoderma asperellum and its application
CN106754426B (en) * 2017-01-17 2020-05-08 中国科学院烟台海岸带研究所 Trichoderma asperellum and application thereof

Also Published As

Publication number Publication date
CN105969672B (en) 2019-03-12

Similar Documents

Publication Publication Date Title
CN104805019B (en) One plant of endogenetic fungus that can promote aleurite montana Nutrient Absorption
CN105543132A (en) Bacillus methylotrophicus YB-F7 and application thereof in preventing plant diseases
CN104004666B (en) One strain can promote the endogenetic fungus of Growth of Chinese Fir
CN105462881A (en) Paenibacillus polymyxa for preventing and treating vertieillium wilt in crops and application of paenibacillus polymyxa
CN108795794A (en) A kind of preparation method and application of bacillus pumilus and its screening technique, microbial inoculum
CN104762219B (en) One plant of endogenetic fungus for promoting aleurite montana biomass to increase under low-phosphorous environment
CN103173364B (en) Endophytic fungus promoting casuarina equisetifolia biomass growth
CN103173359B (en) Endophytic fungus promoting casuarina equisetifolia root system growth effect
CN104004664B (en) One strain can improve the photosynthetic endogenetic fungus of China fir
CN103173361B (en) Endophytic fungus promoting casuarina equisetifolia nutrient element absorption
CN105969672A (en) Mixed endophytic fungi capable of promoting growth of heights and ground diameters of Taiwan acacia seedlings
CN103173360B (en) Endophytic fungus increasing casuarina equisetifolia chlorophyll content
CN106010984B (en) One plant of endogenetic fungus for promoting acacia confusa biomass to increase under low-phosphorous environment
CN106085872B (en) A kind of mixing endogenetic fungus that can promote acacia confusa Nutrient Absorption
CN104789481A (en) Endophytic fungus for promoting growth and photosynthesis enhancement of aleurites montana in low-phosphorus environment
CN104818219B (en) One plant of endogenetic fungus for promoting aleurite montana root growth under low-phosphorous environment
CN104004665B (en) One strain can alleviate the China fir endogenetic fungus of P deficiency
CN103409342A (en) Biocontrol strain YKY01 for preventing and treating aromatic tobacco black shank and anti-biological inoculant of biocontrol strain YKY01
CN106085873B (en) The mixing endogenetic fungus that acacia confusa P elements can be promoted to absorb under low-phosphorous environment
CN115948254B (en) Trichoderma viride 192-45 and application thereof
CN103194396B (en) Aspergillus strain capable of promoting root growth of Casuarina
CN105861334B (en) One plant of endogenetic fungus that acacia confusa biomass can be promoted to increase
CN104818218B (en) One plant can promote the endogenetic fungus that aleurite montana phosphorus absorbs
CN105861335B (en) The endogenetic fungus of one plant of promotion acacia confusa Nutrient Absorption under low-phosphorous environment
CN104774771B (en) One plant promotes the photosynthetic endogenetic fungus of aleurite montana under low-phosphorous environment

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20190312

Termination date: 20210622