CN111527988A - Bud forcing method for edible fungi - Google Patents

Bud forcing method for edible fungi Download PDF

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Publication number
CN111527988A
CN111527988A CN202010567822.3A CN202010567822A CN111527988A CN 111527988 A CN111527988 A CN 111527988A CN 202010567822 A CN202010567822 A CN 202010567822A CN 111527988 A CN111527988 A CN 111527988A
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CN
China
Prior art keywords
edible fungi
bud
edible
aqueous solution
bud forcing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202010567822.3A
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Chinese (zh)
Inventor
雷萍
吴亚召
张文隽
渠敬峰
马婧嘉
钱磊
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Microbiology Institute Of Shaanxi
Original Assignee
Yangling Kangyu Biological Technology Co ltd
SHAANXI PROVINCE INSTITUTE OF MICROBIOLOGY
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Yangling Kangyu Biological Technology Co ltd, SHAANXI PROVINCE INSTITUTE OF MICROBIOLOGY filed Critical Yangling Kangyu Biological Technology Co ltd
Priority to CN202010567822.3A priority Critical patent/CN111527988A/en
Publication of CN111527988A publication Critical patent/CN111527988A/en
Pending legal-status Critical Current

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Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • A01G18/60Cultivation rooms; Equipment therefor
    • A01G18/69Arrangements for managing the environment, e.g. sprinklers

Abstract

The invention belongs to the technical field of edible fungus cultivation, and provides a bud forcing method for edible fungi. The invention utilizes cellulase to perform bud forcing on edible fungi, and provides a bud forcing method for the edible fungi, which comprises the following steps: (1) performing mushroom scratching treatment on the edible mushrooms, and applying cellulase aqueous solution to fruiting surfaces of the edible mushrooms after hypha recovery; (2) spraying water for 2-4 times every day after treatment in the step (1), keeping the ambient humidity at 75-95%, the ambient temperature at 15-30 ℃, windowing and ventilating for 0.5-1.5 h after spraying water for each time, and managing for 3-4 days; (3) and (3) increasing the ventilation quantity according to the method in the step (2), managing for 2-3 days, and ending the bud forcing. The method provided by the invention improves the bud incidence rate of the fungus bag, improves the quality and yield of the edible fungi, and hastens the bud of the edible fungi by spraying the cellulase aqueous solution, so that the management is easy and the bud emergence is stable.

Description

Bud forcing method for edible fungi
Technical Field
The invention belongs to the technical field of edible fungus cultivation, and particularly relates to a bud forcing method for edible fungi.
Background
The bud forcing is a key link in the cultivation and management of the edible fungi, and relates to the yield and quality of the edible fungi. The cultivation bag with good bud promoting effect has the advantages of more and straight mushroom buds, high yield and excellent quality. The cultivation bags with poor bud promoting effect have sparse mushroom buds, a plurality of small cluster mushroom buds dispersed on the surface of the cultivation material, thick stipe and large pileus, and cannot be compensated even if the management is strengthened in the later period. Usually, the bud forcing of edible fungi is mainly controlled by temperature difference, but the temperature difference is an uncontrollable factor in the cultivation of edible fungi in a greenhouse. Once the climatic conditions with too small temperature difference occur in the bud period of the edible fungi, the cultivation bag with poor bud forcing effect is difficult to avoid.
Disclosure of Invention
The invention aims to provide application of cellulase to bud forcing of edible fungi aiming at the problem of poor bud forcing in the production of the edible fungi, and provides a bud forcing method capable of promoting the bud forcing of the edible fungi and improving the yield of the edible fungi.
In order to achieve the above object, the present invention provides the following technical solutions:
the invention provides application of cellulase in bud forcing of edible fungi.
The invention also provides a bud forcing method of edible fungi, which comprises the following steps:
(1) performing mushroom scratching treatment on the edible mushrooms, and applying cellulase aqueous solution to fruiting surfaces of the edible mushrooms after hypha recovery;
(2) spraying water for 2-4 times every day after treatment in the step (1), keeping the ambient humidity at 75-95%, the ambient temperature at 15-30 ℃, windowing and ventilating for 0.5-1.5 h after spraying water for each time, and managing for 3-4 days;
(3) and (3) increasing the ventilation quantity according to the method in the step (2), managing for 2-3 days, and ending the bud forcing.
Preferably, the concentration of the cellulase aqueous solution is 0.5-2 g/L.
Preferably, the cellulase aqueous solution is used in an amount capable of wetting fruiting surfaces.
Preferably, the mode of increasing the ventilation volume is to open the shed and open the window for ventilation after spraying water each time.
Preferably, the edible fungi comprise oyster mushrooms, black fungi, needle mushrooms, pleurotus eryngii and lucid ganoderma.
The application and the method provided by the invention improve the bud incidence rate of the fungus bag and the quality of the edible fungi, and the edible fungi are promoted to bud by spraying the cellulase aqueous solution, so that the management is easy and the bud emergence is stable.
Detailed Description
The invention provides application of cellulase in bud forcing of edible fungi.
The invention also provides a bud forcing method of edible fungi, which comprises the following steps:
(1) performing mushroom scratching treatment on the edible mushrooms, and applying cellulase aqueous solution to fruiting surfaces of the edible mushrooms after hypha recovery;
(2) spraying water for 2-4 times every day after treatment in the step (1), keeping the ambient humidity at 75-95%, the ambient temperature at 15-30 ℃, windowing and ventilating for 0.5-1.5 h after spraying water for each time, and managing for 3-4 days;
(3) and (3) increasing the ventilation quantity according to the method in the step (2), managing for 2-3 days, and ending the bud forcing.
The bud forcing method of the invention is that after edible fungi are subjected to mycelium stimulation treatment and hyphae are recovered, cellulase aqueous solution is applied to fruiting surfaces of the edible fungi.
In the present invention, the treatment of mycelium stimulation is not particularly limited, and the conventional mycelium stimulation of edible fungi may be carried out.
In the invention, the concentration of the cellulase aqueous solution is preferably 0.5-2 g/L, and more preferably 1 g/L.
In the present invention, the cellulase aqueous solution is preferably used in an amount capable of wetting the fruiting surface.
According to the invention, after the fruiting surface is applied with the cellulase aqueous solution, bud forcing management is carried out.
In the invention, water is preferably sprayed into the mushroom shed 2-4 times per day. The humidity of the environment is preferably kept at 75 to 95%, more preferably 80 to 90%, and still more preferably 85%. The times and the interval time of water spraying are determined according to the environmental humidity, and water spraying and humidifying are carried out when the environmental humidity does not meet the requirement.
In the invention, the bud forcing environment temperature is preferably 15-30 ℃, more preferably 18-28 ℃, and still more preferably 25 ℃.
In the present invention, the ventilation time is preferably 0.5 to 1.5 hours, and more preferably 1 hour.
In the present invention, it is preferable to perform window opening ventilation after each water spray every day.
After 3-4 days of management, hypha on the surface of the compost is twisted and water drops appear, ventilation is increased at the moment, more fresh air is fed, dense roe-shaped primordia are promoted to appear on the fruiting surface, after 2-3 days of management, the primordia grow over the surface of the compost and begin to differentiate into mushroom buds, and when the diameter of a pileus reaches 1mm and the length of a stipe is about 1.5mm, bud forcing is finished.
In the invention, the preferable mode of increasing the ventilation quantity is to open the shed and open the window for ventilation after spraying water each time.
In the invention, the edible fungi preferably comprise oyster mushroom, black fungus, needle mushroom, pleurotus eryngii and lucid ganoderma.
The technical solutions provided by the present invention are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention.
Example 1
Transferring the needle mushroom cultivation bag full of mushrooms into a mushroom growing shed, performing mushroom removing treatment on the mushroom bag, spraying 1g/L cellulase aqueous solution once on fruiting surfaces of the edible mushrooms after mycelia are recovered, and wetting the fruiting surfaces by the cellulase aqueous solution. And then carrying out bud forcing management, spraying water 3 times a day, keeping the ground and the surrounding walls moist at 8:30 in the morning, 11:50 in the noon and 3:15 in the afternoon respectively, controlling the environment humidity to be 85% and the temperature to be 25 ℃, spraying water each time every day, then windowing and ventilating for 1 hour, and treating for 3 days. After 3 days of bud forcing management, the hyphae begin to kink and drop. Then under the same air humidity and temperature conditions, the ventilation quantity is increased, and the shed is opened and the window is opened for ventilation for 1 hour after water is sprayed every day. The ventilation quantity is increased, so that more fresh air can enter, the fresh air is contacted in the fungus bags, and the generation of a large number of mushroom buds is promoted. And after 2 days of bud forcing management, the primordium grows over the surface of the culture material and begins to differentiate into mushroom buds, and when the diameter of the pileus reaches 1mm and the length of a stipe is about 1.5mm, the bud forcing is finished. The incidence rate of mushroom buds in the fungus bags is calculated to be 95%, and the yield of the edible fungi is improved by 50%.
Example 2
Transferring the full row pleurotus eryngii cultivation bag into a fruiting shed, carrying out mycelium stimulation treatment on the bag, spraying 0.5g/L cellulase aqueous solution once onto fruiting surfaces of the edible mushrooms after mycelium recovery, and wetting the fruiting surfaces by the cellulase aqueous solution. And performing bud forcing management, spraying water 2 times a day at 10:00 am and 2:10 pm respectively, keeping the ground and the surrounding walls moist, controlling the environment humidity to be 90% and the temperature to be 30 ℃, windowing and ventilating for 1.5 hours after spraying water each time every day, and treating for 4 days. After the bud forcing management for 4 days, the hyphae begin to kink and bead appears. Then under the same air humidity and temperature conditions, the ventilation quantity is increased, and the shed is opened and the window is opened for ventilation for 1.5 hours after water is sprayed every day. The ventilation is increased to allow more fresh air to enter, so that the fresh air is contacted in the fungus bags, and the generation of a large number of mushroom buds is promoted. And after 3 days of bud forcing management, the primordium grows over the surface of the culture material and begins to differentiate into mushroom buds, and when the diameter of the pileus reaches 1mm and the length of a stipe is about 1.5mm, the bud forcing is finished. The incidence rate of mushroom buds in the fungus bags is calculated to be 95%, and the yield of the edible fungi is improved by 20%.
Example 3
Transferring the mushroom cultivation bag full of mushroom into a mushroom growing shed, performing mushroom removing treatment on the mushroom bag, spraying 2g/L cellulase aqueous solution once on the fruiting surface of the edible mushroom after the mycelium is recovered, and wetting the fruiting surface by the cellulase aqueous solution. Then carrying out bud forcing management, spraying water 4 times a day at 8:00 am, 11:15 am, 2:00 pm and 4:30 pm respectively, keeping the ground and the surrounding walls moist, controlling the environment humidity to be 75% and the temperature to be 18 ℃; after spraying water each time every day, opening the window for ventilation for 0.5 hour, and treating for 3 days. After 3 days of bud forcing management, the hyphae begin to kink and drop. Then under the same air humidity and temperature conditions, the ventilation quantity is increased, and the shed is opened and the window is opened for ventilation for 0.5 hour after water is sprayed every day. The ventilation is increased to allow more fresh air to enter, so that the fresh air is contacted in the fungus bags, and the generation of a large number of mushroom buds is promoted. And after 2 days of bud forcing management, the primordium grows over the surface of the culture material and begins to differentiate into mushroom buds, and when the diameter of the pileus reaches 1mm and the length of a stipe is about 1.5mm, the bud forcing is finished. The incidence rate of mushroom buds in the fungus bags is calculated to be 95%, and the yield of the edible fungi is improved by 30%.
According to the embodiment, the invention provides the bud forcing method for the edible fungi, the method for cultivating the oyster mushrooms, the black fungi and the lucid ganoderma can enable the occurrence rate of fungus bag buds to reach 95% and the yield of the edible fungi to be improved by 20-50%, and the method is simple, easy to control and stable in bud emergence.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.

Claims (6)

1. Application of cellulase in promoting edible fungi budding is provided.
2. A bud forcing method of edible fungi is characterized by comprising the following steps:
(1) performing mushroom scratching treatment on the edible mushrooms, and applying cellulase aqueous solution to fruiting surfaces of the edible mushrooms after hypha recovery;
(2) spraying water for 2-4 times every day after treatment in the step (1), keeping the ambient humidity at 75-95%, the ambient temperature at 15-30 ℃, windowing and ventilating for 0.5-1.5 h after spraying water for each time, and managing for 3-4 days;
(3) and (3) increasing the ventilation quantity according to the method in the step (2), managing for 2-3 days, and ending the bud forcing.
3. The bud forcing method for edible fungi as claimed in claim 2, wherein the concentration of the cellulase aqueous solution is 0.5-2 g/L.
4. A method for inducing primordium of edible fungi according to claim 2 or 3, wherein the aqueous solution of cellulase is used in an amount capable of wetting the fruiting surface.
5. The method for inducing primordium of edible fungi according to claim 2, wherein the ventilation quantity is increased by opening a shed and opening windows for ventilation after each water spraying.
6. The method according to claim 2, wherein the edible fungi comprise Pleurotus ostreatus, Lentinus edodes, Auricularia, Flammulina velutipes, Pleurotus eryngii, and Ganoderma lucidum.
CN202010567822.3A 2020-06-19 2020-06-19 Bud forcing method for edible fungi Pending CN111527988A (en)

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CN202010567822.3A CN111527988A (en) 2020-06-19 2020-06-19 Bud forcing method for edible fungi

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CN202010567822.3A CN111527988A (en) 2020-06-19 2020-06-19 Bud forcing method for edible fungi

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Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CH660832A5 (en) * 1983-06-16 1987-05-29 Interfive Ltd Process for growing mycelium on a cellulose substrate
CN106576899A (en) * 2016-11-24 2017-04-26 凤台县兰韵食用菌专业合作社 Cultivation method capable of facilitating straw mushroom primordium differentiation
US20180000013A1 (en) * 2016-02-26 2018-01-04 Functional Fungi, Llc Enhanced Mycelium Growth Medium and Method
CN107950301A (en) * 2017-12-26 2018-04-24 大连民族大学 A kind of composting method of Alfalfa mushroom compost
CN108739067A (en) * 2018-06-06 2018-11-06 湖南科技大学 A kind of method and application shortened oyster mushroom liquid spawn and be inoculated with bacterium bag bacteria developing period
CN108849241A (en) * 2018-07-12 2018-11-23 通化县金汇生态农业发展有限公司 A method of utilizing agricultural crop straw culturing edible fungus
CN110249911A (en) * 2019-07-28 2019-09-20 王问渠 A kind of oyster mushroom flower bud method

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CH660832A5 (en) * 1983-06-16 1987-05-29 Interfive Ltd Process for growing mycelium on a cellulose substrate
US20180000013A1 (en) * 2016-02-26 2018-01-04 Functional Fungi, Llc Enhanced Mycelium Growth Medium and Method
CN106576899A (en) * 2016-11-24 2017-04-26 凤台县兰韵食用菌专业合作社 Cultivation method capable of facilitating straw mushroom primordium differentiation
CN107950301A (en) * 2017-12-26 2018-04-24 大连民族大学 A kind of composting method of Alfalfa mushroom compost
CN108739067A (en) * 2018-06-06 2018-11-06 湖南科技大学 A kind of method and application shortened oyster mushroom liquid spawn and be inoculated with bacterium bag bacteria developing period
CN108849241A (en) * 2018-07-12 2018-11-23 通化县金汇生态农业发展有限公司 A method of utilizing agricultural crop straw culturing edible fungus
CN110249911A (en) * 2019-07-28 2019-09-20 王问渠 A kind of oyster mushroom flower bud method

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
袁瑞奇 等: "《杏鲍菇栽培实操技术图解》", 28 February 2015, 中原农民出版社 *
辛宇: "两种食用菌栽培基质中酶制剂的施用效果研究", 《中国优秀硕士学位论文全文数据库 农业科技》 *

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Effective date of registration: 20220815

Address after: 710043 Shaanxi province Xi'an Xiying Road No. 8

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