CN111493220A - Lactation promoting paste for dogs and cats - Google Patents
Lactation promoting paste for dogs and cats Download PDFInfo
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- CN111493220A CN111493220A CN202010322911.1A CN202010322911A CN111493220A CN 111493220 A CN111493220 A CN 111493220A CN 202010322911 A CN202010322911 A CN 202010322911A CN 111493220 A CN111493220 A CN 111493220A
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/40—Feeding-stuffs specially adapted for particular animals for carnivorous animals, e.g. cats or dogs
- A23K50/45—Semi-moist feed
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/158—Fatty acids; Fats; Products containing oils or fats
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
Abstract
The invention relates to a lactation promoting paste for dogs and cats, and solves the problem that the lactation amount of the existing lactation period dog food is reduced due to the fact that the appetite of a female dog is easily reduced due to the smell of traditional Chinese medicine components. The paint comprises the following components in parts by weight: 30-45 parts of small red beans, 5-15 parts of angelica sinensis, 5-18 parts of astragalus membranaceus, 5-20 parts of motherwort, 10-25 parts of wolfberry fruits, 100-150 parts of brown sugar, 110-160 parts of amino acids, 60-100 parts of fatty acids, 120-180 parts of vitamin complex minerals, 180-250 parts of matrix, 10-25 parts of forming agent and 200-250 parts of water. The invention has the effects of improving the physical function of the post-partum bitch (cat) and increasing the lactation yield of the post-partum bitch (cat), provides energy, vitamins and minerals for the post-partum bitch (cat), provides nutrient substances required by the body, and also has the effect of improving the content of the nutrient substances in milk.
Description
Technical Field
The invention relates to the technical field of pet nutritional supplements, in particular to a cream for promoting dogs and cats.
Background
The Chinese herbal medicine is a cultural treasure left in China for thousands of years, has the health-care pharmacological effects of strengthening spleen and stimulating appetite, tonifying liver and kidney, regulating qi and activating blood, calming nerves and centering, nourishing blood and tonifying qi, preventing diseases in active service and the like, contains a certain amount of nutrient substances, contains animal growth promoting factors, and has the effects of protecting health, stimulating appetite, helping digestion, promoting growth, improving production function, reducing feed consumption and the like when being added into feed.
Chinese herbal medicines are used as livestock and poultry feed in China for a long time, most of the Chinese herbal medicines contain natural nutrient substances, are low in toxicity and side effects, and are mixed and added into the feed in combination with the physiological characteristics of livestock and poultry and the concrete practical effects so as to enhance the growth function of the livestock and poultry. With the development of the pet market, more and more people begin to raise pets, dogs and cats are popular among people as pets which are easy to care and have strong resistance, and dogs and cats need to feed cubs after delivery, so that the demand for nutrient substances is large. According to the ordinary feeding mode, the lactation amount of dogs and cats can not meet the requirement of cubs.
The Chinese invention application with the application publication number of CN104509744A discloses a lactation dog food which comprises the following components in parts by mass: 5-8 parts of Chinese cabbage, 8-12 parts of carrot, 10-15 parts of corn, 30-40 parts of wheat, 5-15 parts of millet, 12-15 parts of angelica sinensis, 5-7 parts of codonopsis pilosula, 5-6 parts of astragalus membranaceus, 5-7 parts of beautiful sweetgum fruit, 3-5 parts of dried orange peel, 5-7 parts of white paeony root, 15-30 parts of fish meat, 5-10 parts of beef, 5-8 parts of chicken, 5-8 parts of beef bone, 2-3 parts of chicken bone and 0.3-0.5 part of egg. According to the lactation promoting dog food, the traditional Chinese medicine components which are beneficial to lactation such as the angelica sinensis and the astragalus membranaceus are added, so that the lactation promoting effect of the dog food is achieved, but the lactation promoting effect is to be improved.
Disclosure of Invention
The invention aims to provide a cream for dogs and cats, which has the advantages that: has the effects of promoting lactation and promoting lactation, and can remarkably improve the lactation yield and the milk quality of the bitch (cat).
The above object of the present invention is achieved by the following technical solutions:
the cream promoter for dogs and cats comprises the following components in parts by weight: 30-45 parts of small red beans, 5-15 parts of angelica sinensis, 5-18 parts of astragalus membranaceus, 5-20 parts of motherwort, 10-25 parts of wolfberry fruits, 100-150 parts of brown sugar, 110-160 parts of amino acids, 60-100 parts of fatty acids, 120-180 parts of vitamin complex minerals, 180-250 parts of matrix, 10-25 parts of forming agent and 200-250 parts of water.
By adopting the technical scheme, small red beans, angelica sinensis, astragalus membranaceus, motherwort and wolfberry fruits are ground into powder, then the powder is uniformly mixed with brown sugar, amino acid, fatty acid, matrix and vitamin complex minerals, then water is added, the mixture is uniformly stirred, and finally a forming agent is added, the mixture is stirred and mixed into paste.
The small red bean has the effects of promoting blood circulation, enriching blood, inducing diuresis, reducing edema and promoting lactation, the Chinese angelica has the effects of enriching blood, promoting blood circulation and promoting the hematopoietic function of organisms, and the motherwort has the effect of promoting blood circulation and can improve the lactation yield of a bitch (cat); radix astragali has effects of invigorating spleen and replenishing qi, and fructus Lycii has effects of nourishing liver and kidney, and can improve puerperal asthenia of female dog (cat); the brown sugar contains abundant trace element components, has the effects of tonifying qi and enriching blood, stimulating the hemopoiesis of organisms, improving the physical function of the female dog (cat) after delivery, being beneficial to the rapid recovery of the female dog (cat) after delivery and being beneficial to lactation.
The amino acid is used for ensuring the normal operation of postpartum physiological function and body metabolism of a female dog (cat), providing energy, improving the body resistance, promoting the secretion of milk and improving the content of nutrient components in the milk, for example, the valine can improve the content of milk protein and milk fat in the milk and improve the nutrient value of the milk, the L-lysine can provide nutrient components for the female dog (cat), promote the fat metabolism and improve the body resistance of the female dog (cat), and the L-arginine is used for promoting the formation of urea and keeping the body balance.
The vitamin complex minerals provide abundant vitamins and minerals for a post-partum female dog (cat), provide nutrients required by the body, and promote carbohydrate metabolism, protein synthesis and carbohydrate decomposition, such as: the vitamin A can improve the immunity of postpartum organisms of the bitch (cat), promote the generation of hemoglobin and the absorption of iron, and is beneficial to lactation; the vitamin D can improve the absorption and metabolism of calcium and phosphorus of the bitch (cat), prevent acute postpartum calcium deficiency caused by too low blood calcium concentration of the bitch (cat), avoid the phenomena of four limbs convulsion, whole body stiffness and the like of the bitch (cat), and enhance the physique of the bitch (cat); the vitamin E can promote sex hormone secretion and improve lactation yield of female dogs (cats).
The fatty acid is used for providing body energy for the postpartum canines (cats) so as to keep the canines (cats) in a good physical state; the matrix is used for improving the taste and palatability of the lactation promoting cream, increasing the appetite and intake of the bitch (cat), and being beneficial to improving the lactation yield and milk quality of the bitch (cat); the forming agent is used for mixing and forming the components of the cream to be paste, is convenient for packaging and storage and is convenient for the female dog (cat) to eat.
The lactation promoting paste obtained by combining the components has the effects of improving the postpartum body weakness state of the female dog (cat), improving the body function of the female dog (cat), accelerating the postpartum recovery speed of the female dog (cat), improving the lactation yield of the female dog (cat), providing energy, vitamins and minerals for the female dog (cat) after parturition, providing nutrient substances required by the body, simultaneously improving the content of the nutrient substances in milk and enhancing the resistance and immunity of the puppy (cat), and is beneficial to the healthy growth of the puppy (cat).
The amino acid composition further comprises, by weight, L-arginine 6-8.5 parts, histidine 3-4.5 parts, L-leucine 6-12 parts, isoleucine 10-20 parts, valine 35-50 parts, L-lysine 5-11 parts, D L-methionine 25-35 parts, L-threonine 7-14 parts, phenylalanine 5-10 parts and L-tryptophan 1-3 parts.
By adopting the technical scheme, the histidine is used for relaxing blood vessels, accelerating blood circulation and promoting lactation, L-leucine and isoleucine can provide energy for a bitch (cat) after delivery and are favorable for lactation, meanwhile, the isoleucine has high nutritional value and can improve the content of nutritional ingredients in milk and is favorable for growth of a puppy (cat), D L-methionine and L-threonine are used for improving amino acid balance and promoting protein synthesis, the phenylalanine can promote carbohydrate metabolism and fat metabolism of an organism and provide energy for the organism, and the L-tryptophan can promote plasma protein metabolism in the bitch (cat), improve resistance and promote lactation, and the amino acid combination enables the cream to improve the resistance of the bitch (cat) organism, promote lactation and improve the content of the nutritional ingredients in the milk.
The invention is further configured to: the fatty acid comprises the following components in parts by weight: 35-55 parts of fish oil and 25-45 parts of chicken oil.
By adopting the technical scheme, the fish oil and the chicken oil contain rich fatty acid, so that the lactation promoting paste can provide a large amount of energy for postpartum canines (cats), improve the body functions of the canines (cats) and improve the lactation yield.
The invention is further configured to: the vitamin and mineral complex comprises the following components in parts by weight: 1-3.5 parts of vitamin A, 0.5-1 part of vitamin D, 4-8 parts of vitamin E, 10.3-0.7 part of vitamin B, 20.5-1 part of vitamin B, 60.1-0.25 part of vitamin B, 1-3 parts of nicotinic acid, 0.7-1.3 parts of pantothenic acid, 120.3-0.5 part of vitamin B, 0.04-0.055 part of folic acid, 0.001-0.0012 part of biotin, 12-17 parts of choline chloride, 74-87 parts of milk calcium, 7-12 parts of magnesium sulfate, 8-13 parts of ferrous lactate, 8-12 parts of zinc gluconate and 7-13 parts of selenium-enriched yeast (2000 ppm/kg).
By adopting the technical scheme, the vitamin B1 can improve the digestion capacity of the bitch (cat) on carbohydrate, and is beneficial to providing energy for organisms; the vitamin B2 can promote metabolism of the body of the bitch (cat), and improve the absorption and utilization of the body to protein; nicotinic acid is used for promoting the metabolism of lipids and the decomposition of saccharides in a bitch (cat); pantothenic acid is used for promoting energy production and fat metabolism in vivo; the vitamin B12 can promote the development and maturation of erythrocytes, improve the hematopoietic function of the body, facilitate lactation and promote the metabolism of carbohydrates, fat and proteins; the folic acid and the vitamin B12 can jointly promote the generation of red blood cells, is beneficial to lactation and improves the immunity of the organism; the biotin is used for promoting the metabolism of fat and protein of the organism and enhancing the immunity and resistance of the organism; the choline chloride can enhance the postpartum physique and resistance of the female dog (cat), improve the utilization rate of amino acid in the body, promote the body development and improve the lactation yield.
The milk calcium contains rich nutrient components such as mineral substances, proteins, vitamins and the like, improves the quality and the nutritional value of the milk, contains the calcium content which is easy to be absorbed by the body, can quickly supplement the calcium ions which are lacked in the mother body, and avoids acute postpartum calcium deficiency caused by the low blood calcium concentration of the female dog (cat); magnesium sulfate is used for providing magnesium element, ferrous lactate is used for providing iron element, and the metabolism of carbohydrate and protein in a body and the generation of hemoglobin are promoted; the zinc gluconate can provide organic zinc, the selenium-enriched yeast is used for providing biological selenium, the synthesis of nucleic acid and protein is promoted, the immunity of an organism is enhanced, the organic zinc and the biological selenium can greatly reduce the stimulation to intestines and stomach, the absorption and the utilization are easy, and the quality of milk is improved.
The addition of the compound vitamin mineral enables the lactation promoting cream to provide abundant vitamins and minerals for the female dogs (cats) after delivery, provide nutrient substances required by organisms and promote lactation.
The invention is further configured to: the components of the matrix in parts by weight comprise: 55-65 parts of concentrated whey protein, 55-70 parts of goat milk powder, 25-35 parts of chicken powder, 25-40 parts of chicken liver powder and 25-40 parts of crucian meat powder.
By adopting the technical scheme, the concentrated whey protein has rich types and contents of amino acids, high nutritional value, easy digestion and absorption, improved milk quality, contribution to the growth of larvae, sufficient protein content in the concentrated whey protein, capability of enabling the lactation promoting paste to provide body energy for the bitch (cat), and improved immunity and resistance; the goat milk powder contains a large amount of whey protein, minerals, vitamins and trace elements, has high nutritional value, does not contain foreign protein, is easier to digest and absorb, does not cause discomfort of intestines and stomach of a larva, and improves the quality of milk; the chicken powder, the chicken liver powder and the crucian carp powder contain more nutrient components, are beneficial to lactation of a bitch (cat), can improve the taste and the palatability of the galactagogue, promote the appetite of the bitch (cat), and ensure the intake of the bitch (cat) to improve the lactation amount and the milk quality.
The invention is further configured to: the components of the forming agent in parts by weight comprise: 2-3.5 parts of edible gelatin and 12-18.5 parts of sodium carboxymethylcellulose.
By adopting the technical scheme, the edible gelatin and the sodium carboxymethyl cellulose play a role of a food thickener and are used for bonding the components of the cream promoter together to form a paste; the edible gelatin is high in protein, rich in amino acid, free of fat and cholesterol, easy to absorb, strong in emulsifying capacity, and capable of inhibiting protein coagulation caused by gastric acid, thereby being beneficial to absorption and digestion of milk; the sodium carboxymethylcellulose has good emulsification stabilizing effect, keeps the catalytic cream in a paste shape, can improve the palatability of the catalytic cream and prolongs the storage time.
In conclusion, the beneficial technical effects of the invention are as follows:
the small red bean, the Chinese angelica, the motherwort, the astragalus and the medlar are used as raw materials of traditional Chinese medicinal materials and added into the brown sugar, so that the lactation promoting paste has the effects of tonifying qi and activating blood, promoting the hematopoietic function of organisms, promoting lactation and the like, can improve the postpartum body weakness state of a bitch (cat), strengthen the physical state after delivery of the bitch (cat), further improve the lactation yield of the bitch (cat), and the brown sugar in the lactation promoting paste contains trace element components, can stimulate the hematopoietic function of the organisms, can improve the postpartum body function of the bitch (cat), and is beneficial to lactation.
The amino acid and the fatty acid in the lactation promoting paste are used for ensuring the normal operation of postpartum physiological functions and body metabolism of the bitch (cat), providing energy and improving the body resistance; the vitamin and mineral complex in the lactation promoting paste provides rich vitamins and minerals for the postpartum female dogs (cats), provides nutrients required by the body, and promotes the metabolism of carbohydrates, the synthesis of proteins and the decomposition of saccharides.
Along with the increase of lactation amount of a female dog (cat), the milk intake amount of a puppy (cat) can be improved, the growth of the puppy (cat) is facilitated, meanwhile, the amino acid and the vitamin complex mineral substance in the lactation promoting paste can also improve the content of nutrient substances in the milk, the resistance and the immunity of the puppy (cat) can be enhanced, the physical function of the puppy (cat) is improved, the possibility of illness of the puppy (cat) is reduced, and the healthy growth of the puppy (cat) is facilitated.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the tables in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, other embodiments obtained by persons of ordinary skill in the art with the understanding of the inventive concept of the present invention are within the scope of the present invention.
The invention discloses a cream for promoting dogs and cats, which comprises the following raw materials in parts by weight: 30-45 parts of small red beans, 5-15 parts of angelica sinensis, 5-18 parts of astragalus membranaceus, 5-20 parts of motherwort, 10-25 parts of wolfberry fruits, 100-150 parts of brown sugar, 110-160 parts of amino acids, 60-100 parts of fatty acids, 120-180 parts of vitamin complex minerals, 180-250 parts of matrix, 10-25 parts of forming agent and 200-250 parts of water.
In order to facilitate understanding of the technical solution of the present invention, the cream for promoting dogs and cats provided by the present invention is further described in detail below, but is not intended to limit the scope of the present invention.
The specific embodiment is as follows:
1. experimental animals and groups
The experiment picks medium-sized dams with similar body conditions, the dams are grouped according to the average weight of cubs after the dams leave the cubs, the dams are divided into a control group A and an experimental group B-an experimental group H, each group is provided with 8 dams, the experiment is also suitable for cats, and the detection result can be analogized to the cats.
2. Feeding management
The feed of the female dogs from pregnancy to lactation period is the same; recording the birth day of the cubs as 1 day old, and carrying out cross breeding in groups; after 7 groups of experimental group dogs farrowing and colostrum collection, except for daily diet feeding, feeding was respectively carried out according to the components of the emulsifiable concentrates in different weight parts (using amount: 0.5g of ointment/kg of body weight, once a day), and the control group A was not fed with the emulsifiable concentrates, but fed with daily diet.
3. Milk sample Collection
Collecting milk respectively within 24h after delivery of the female dog and at 7 th, 14 th and 21 th days of lactation, collecting milk from the front, middle and rear nipples of the female dog respectively, mixing, wherein each milk sample is 15ml, and placing the milk samples in a refrigerator at-80 ℃ for storage to be tested.
4. Index and method of measurement
4.1. Lactation volume of bitch
Milk is collected for measurement within 24h after delivery of the female dog and at 7 th, 14 th and 21 th days of lactation, and the lactation amount is obtained.
4.2. Weight of baby
The weights of the pups were measured at 1 day, 7 days, 14 days, and 21 days of age, and the weights were calculated by comparing the pups at 7 days, 14 days, and 21 days of age with the pups at 1 day of age.
4.3. Milk ingredient determination
And comparing according to the lactation promoting effect, selecting a group of components with better lactation promoting effect in parts by weight for milk component test analysis, and respectively analyzing and determining the content of milk protein, the content of milk fat and the content of non-fat solids in the group of milk.
The measuring instrument adopts a full-automatic breast milk analyzer (model: HY-MR 600) produced by Jining Huayang flourishing medical science and technology Limited.
The specific experimental data is shown in the following table (all data processing was analyzed by means of the software IBM SPSS Statistics 20):
feeding the bitches with the following 7 weight parts of prolactin emulsion respectively:
table 1: raw material components of the cream promoter
Under the same conditions, the lactation amount of the canines in the control group A which are not fed with the prolactin are used as comparison, and then the lactating creams of the components in the weight parts of the embodiments 1 to 7 are respectively fed to the canines in the experimental groups B to H to carry out comparison experiments, so as to compare the control group A.
Table 2: lactation volume of the bitch within 24h, within 7 days, within 14 days and within 21 days after delivery at the same time (n-8,
)
note: p < 0.05 indicates significant difference, and p < 0.01 indicates extremely significant difference.
From the data analysis of table 2, it can be seen that: after the parturited bitches in the experimental groups B-H eat the lactation promoting paste, the average lactation amount in 7-21 days after parturition is larger than that of the bitches which do not eat the lactation promoting paste in the control group A, and the lactation amount of the bitches which eat the lactation promoting paste at each measurement time node is also larger than that of the bitches which do not eat the lactation promoting paste, so that the lactation promoting paste can play a role in promoting lactation of the parturited bitches, and is favorable for increasing the lactation amount of the parturited bitches.
The lactation promoting effect of the lactation promoting cream on the bitches at the same time within 24 hours is not obvious when the bitches after delivery of the control group A and the experimental groups B-H are parted (the contents are uniform, p is more than 0.05), the data in the table 1 and the table 2 are combined, the data in the experimental group B and the experimental group C are compared, the lactation promoting cream used by matching and mixing the amino acid content is higher, the lactation promoting effect of the lactation promoting cream on the bitches is obvious when the amino acid content is higher, the data in the experimental group C and the experimental group D are compared, L-arginine, valine, L-lysine and vitamin are matched, the lactation promoting cream has obvious difference on the lactation promoting amount (the contents are uniform, p is less than 0.05) when the amino acid content and the vitamin content are compared, the lactation promoting cream used for the bitches is proved to be beneficial to lactation, the data in the experimental group E, the experimental group F and the experimental group G are compared, the data in the cream group E, the experimental group F and the experimental group G are proved that the contents of leonurus and the cream have influence on the lactation promoting effect on the lactation promoting cream on the lactation promoting effect when the contents of the cream is increased, the lactation promoting cream and the fish oil content of the experimental group is known to be beneficial to be improved when the lactation promoting cream and the lactation promoting oil content of the experimental group H.
As can be seen from the data in the above experiments, when the weight parts of the components of the galactagogue are the same as the ratio in example 4, the galactagogue has a good galactagogue effect (P is less than 0.01, and the mean value of the lactation amount of example 4 is the largest), and the lactation amount of the mare is remarkably different (the variance is uniform, and P is less than 0.01).
Comparative experiments were carried out with 5 groups of catalytic emulsions of example 3 to example 7 with different parts by weight:
table 3: the effect of the creams of examples 3 to 7 on the weight gain of pups (n 8,
)
note: p < 0.05 indicates significant difference, and p < 0.01 indicates extremely significant difference.
From the data analysis of table 3, it can be seen that: the weight of the control group and the puppies of examples 3-7 at birth were not significantly different; at each measurement time node of the weight of the puppy at birth, the weight of the puppy fed by the bitch fed with the emulsifiable paste is greater than that of the puppy fed by the bitch not fed with the emulsifiable paste, so that the emulsifiable paste can increase the content of nutrient substances in the bitch and has a beneficial effect on the growth of the puppy;
examples 3 to 5 show that the accelerating creams with the components of the weight parts in examples 6 to 7 have better accelerating effect on the growth of puppies than the accelerating creams with the components of the weight parts in examples 6 to 7 (with uniform variance, p < 0.05 and obvious difference), which indicates that the effect of the accelerating creams can be maximized under the condition that the red bean, the angelica, the astragalus, the motherwort and the brown sugar are in proper proportion; the effect of the cream promoter of the component in the weight part in the example 4 is better, and the cream promoter is more beneficial to the growth of puppies (the variance is uniform, p is less than 0.01, the difference is extremely obvious, and the average value of the weight gain of pups in the example 4 is the highest), so that the effect of the cream promoter of the component in the weight part in the example 4 is the best one of the effect of the cream promoter of the 5 different weight parts.
Since the galactagogue of example 4 has a good galactagogue effect, the milk secreted by the dogs fed with the galactagogue of this weight fraction is used as a sample, the milk protein, milk fat and non-fat solids in the milk are measured and analyzed, and the milk secreted by the dogs not fed with the galactagogue is selected as a comparative sample for measurement and analysis, and the specific data are as follows:
table 4: comparison of the milk protein, milk fat and non-fat solids content in the milk of the bitches of control group a and experimental group E (with the caltrops of the composition of example 4) (n-8,
)
note: p < 0.05 indicates significant difference, and p < 0.01 indicates extremely significant difference.
From the data in table 4, it can be seen that: the contents of milk proteins, milk fat and non-fat solids in the milk secreted 24h after delivery did not differ significantly between the dogs fed the prolactin and the dogs not fed the prolactin (well-formed variance, p > 0.05), but the milk proteins, milk fat and non-fat solids in the milk secreted 7 days after delivery were all higher than in the milk secreted by the dogs not fed the prolactin, and the milk proteins, milk fat and non-fat solids in the milk secreted at both the nodes 14 days after delivery and 21 days after delivery were all higher than in the milk secreted by the dogs not fed the prolactin, as can be seen from the p-value (p < 0.05) in combination with the fact that the prolactin significantly increased the milk protein, milk fat and non-fat solids in the milk of the dogs, further improving the physique of the puppy at the early growth stage, enhancing the resistance and the immunity of the puppy, reducing the possibility of the disease occurrence of the puppy and being beneficial to the healthy growth of the puppy.
The detection results of the experiments can be similar to those of cats, and in conclusion, the lactation promoting paste can improve the lactation yield of the bitch (cat) and the content of nutrient substances in milk, so that the resistance and the immunity of the puppy (cat) are enhanced, and the healthy growth of the puppy (cat) is facilitated.
In order to test the safety of the invention and verify the influence of the toxicity of the traditional Chinese medicine components on the growth of the dog and cat organisms, the following experiment is designed: a 30-day feeding experiment (short-term oral toxicity experiment, subacute toxicity experiment, reference GB/T23179-.
1. Laboratory animals and apparatus
1.1. Test article
And (3) cream acceleration: sample batch number: 20190722, respectively; sample properties: and (4) ointment.
The usage and dosage are as follows: the weight of the dog or cat is less than 5kg, 1ml is fed every time, the weight of the dog or cat is 5-10 kg, 2ml is fed every time, the weight of the dog or cat is more than 10kg, 3-4 ml is fed every time, and the weight is twice a day.
The syringe-contained product was measured to weigh about 1g per 1m L bolus of the columnar paste extruded from the nozzle, and for ease of handling the experiment, it was now defined that dogs and cats weigh 5kg and were fed 0.4g/kg.BW per day at a dose of paste extruded by pushing the syringe 2m L, thus rats were tested at the basal recommended dose of 40mg/100 g.BW.
1.2. Experimental animals and experimental environment
64 SPF-level weaning SD rats (healthy, half female and half male, 3-4 weeks old) are selected and provided by the Guangdong province medical experimental animal center (license number: SCXK (Guangdong) 2013-.
The experimental animal is fed and observed for 7-10 days before the experiment to adapt to the feeding environment, and the experimental animal which is unhealthy or has unqualified weight is eliminated (the elimination standard is referred to GB/T231798.4-2008), and the experimental environment temperature is as follows: 20-26 ℃, relative humidity: 50-70%. The daily feed for feeding (general-grade rat maintenance compound feed, quality certification number: No. 44200300012363; ingredient information, see table 5) was provided by the Guangdong province medical laboratory animal center.
1.3. Experimental equipment
Mouse cage, injector, stomach filling needle, electronic scale (Guangzhou jade instrument Co., Ltd., model: JY-10002), beaker, reagent bottle, full-automatic biochemical analyzer (Tianjin micro nano core technology Co., Ltd., model: V3), full-automatic blood cell analyzer (Tianjin micro nano core technology Co., Ltd., model: VH3) and related instruments for dissection.
Table 5: ingredient information of compound feed for maintaining common rats
2. Experimental protocol
2.1. Acute toxicity test
2.1.1. The experimental period is as follows: 4 weeks.
2.1.2. Experimental methods
Acute toxicity test is carried out by oral acute toxicity test in feed GB/T15193.3-2003 and related documents. After adaptive feeding, the rats were randomly divided into 8 groups by weight, 1 control group and 7 experimental groups, each group being equipped with 4 female and male rats. The split-flow accelerating cream for the experimental rats is respectively shown in examples 1-7, and the test substances are administered by liquid drenching, wherein distilled water is drenched for the control group, the self-prepared liquid with the same volume is drenched for the experimental rats, and the corresponding liquid is 1ml/100g. Each group of rats was fed in a single cage and fed with free access to food and water (distilled water), the formulations are as shown in Table 6.
Table 6: preparation of each liquid and gavage amount
2.1.3. Observation index and evaluation
The rats were observed for abnormal reactions (up, lying, pale concha or hyperemia, exophthalmos, teetering, muscle paralysis, dyspnea, coma, convulsion, incontinence of stool and urine, etc.) within 2 weeks, and were examined for death and reason for death, and the dead mice were immediately necropsied.
2.2. Thirty days feeding experiment
2.2.1. The experimental period is as follows: for 30 days.
2.2.2. Experimental methods
The method for grouping and preparing the liquid is carried out according to the feeding experiments (short-term oral toxicity experiments and preliminary efficacy experiments) for 30 days in feed GB/T23179-.
Data collection: observing the growth and development, activity and weight change of the mice within 30 days, weighing the weight at least once per week, adjusting the sample amount according to the weight, killing the animals continuously for 30 days at 31 days, taking blood for routine blood detection, taking main organs to measure organ coefficients and performing pathological examination. The results of the analysis were analyzed for variance and correlation using SPSS 19.0 statistical software, and the results were expressed as (x ± s, test level p is 0.05.
2.2.3. Observation index and evaluation
Growth status, body weight and food availability: and observing the conditions of the activity, the eating, the drinking and the defecation of the rat within 30 days, and calculating the weight, the weight increment, the food intake and the food utilization rate.
Hematology and blood biochemistry index on day 1 after 30 days of feeding withdrawal, the tail of each group of rats was sampled and submitted for blood routine examination, such as hemoglobin (Hb), red blood cell count (RBC), white blood cell count (WBC) and classification, etc., using a full-automatic blood cell analyzer, and blood biochemical examination, such as total protein (Tp), glutamic-pyruvic transaminase (A L T), urea nitrogen (BUN), Creatinine (CRE), etc., using an automatic biochemical analyzer.
And C, performing autopsy, namely performing autopsy after all rats are quickly killed by a cervical dislocation method on the 1 st day after feeding is stopped for 30 days, observing pathological changes of heart, liver, spleen, lung, kidney, stomach, intestine and the like, measuring the body coefficient values of liver, kidney, spleen, testis (male) and ovary (female), and calculating the body coefficient (organ/body weight × 100%).
3. Results of the experiment
3.1. Thirty days feeding experimental results
3.1.1. General observations
During the experiment, the rats in each group grow well, and the behavior and activity, mental status, hair color, food and water intake and defecation are normal, so that the galactagogue has no adverse effect on the general conditions of the rats in each group.
3.1.2. Influence on body weight
According to the data in table 7, the body weights and total weight gains of male and female rats in each experimental group are compared with those of the control group without significant difference (with uniform variance, p > 0.05), and it is known that the body weight increase and growth and development of SD rats are not affected by the continuous feeding of the lactation promoting agent in examples 1-7 for 30 days.
Table 7: effect of the prohibition on weight gain in SD rats (n-4,
)
note: indicates significant correlation at the p-0.05 level (bilateral), as follows.
3.1.3. Influence on food utilization
According to the data in Table 8, the food utilization rate of rats in each experimental group was compared with that of the control group, and it was found that the SD rats were not affected by the continuous feeding of the creams of examples 1 to 7 for 30 days.
Table 8: effect of the galactagogue on food availability in SD rats (n-4,
)
3.1.4 Effect on hematology
From the data in table 9, it is clear that the blood routine indicators such as white blood cell count, lymphocyte, intermediate cell, granulocyte and ratio thereof, red blood cell count, hemoglobin, etc. of the rats in each experimental group are not significantly different (p > 0.05) from those in the control group, and that none of the creams of examples 1 to 7 has any adverse effect on the basic indicators of the hematology of SD rats.
Table 9: effect of the galactagogue on hematology in SD rats (n-4,
)
3.1.5. influence on blood biochemistry
According to the data in table 10, when the blood biochemical indexes such as serum total protein, glucose, urea nitrogen, glutamic-pyruvic transaminase, alkaline phosphatase, creatinine, urea nitrogen/creatinine, etc. of rats in each experimental group were compared with those of the control group, no significant difference (p > 0.05) was observed, and it was found that the creams of examples 1 to 7 did not have any adverse effect on the main indexes of blood biochemistry of SD rats.
Table 10: the effect of the prohibiting cream on blood biochemistry in SD rats (n-4,
)
3.1.6. influence on major visceral organs of SD rats
The rats in each experimental group were anatomically compared with the rats in the control group, and no obvious macroscopic lesions were found. From the data in tables 11 and 12, it was found that the weight and coefficient of the organ of the rats in each experimental group were not significantly different from those of the control group (p > 0.05), and the creams in examples 1 to 7 had no significant adverse effect on the weight and coefficient of the organ of the SD rat.
Table 11: SD rats each group weight (n-4,
)
table 12: the organ coefficients of each group of SD rats (n-4,
)
in conclusion, the experiments show that the cream catalysis in examples 1 to 7 has no adverse effect on the growth and development, behavior and activity, mental status, hair color, drinking water and urine of SD rats after being continuously fed for 30 days, has no adverse effect on the weight increase, food utilization rate, hematology index and development, weight and coefficient of main organs of SD rats, and the fact that the cream catalysis in each half of the SD rats has no effect on the growth of male and female individuals in pups of dogs and cats can be inferred, so that the toxicology of the traditional Chinese medicine components in the invention has no adverse effect on the growth of the bodies of dogs and cats.
The embodiments of the present invention are preferred embodiments of the present invention, and the scope of the present invention is not limited by these embodiments, so: all equivalent changes made according to the principles of the present invention should be covered by the protection scope of the present invention.
Claims (6)
1. The cream for dogs and cats is characterized in that: the cream promoter comprises the following components in parts by weight: 30-45 parts of small red beans, 5-15 parts of angelica sinensis, 5-18 parts of astragalus membranaceus, 5-20 parts of motherwort, 10-25 parts of wolfberry fruits, 100-150 parts of brown sugar, 110-160 parts of amino acids, 60-100 parts of fatty acids, 120-180 parts of vitamin complex minerals, 180-250 parts of matrix, 10-25 parts of forming agent and 200-250 parts of water.
2. The cream for dogs and cats as claimed in claim 1, wherein the amino acid comprises L-arginine 6-8.5 parts, histidine 3-4.5 parts, L-leucine 6-12 parts, isoleucine 10-20 parts, valine 35-50 parts, L-lysine 5-11 parts, D L-methionine 25-35 parts, L-threonine 7-14 parts, phenylalanine 5-10 parts, L-tryptophan 1-3 parts.
3. The creotropic cream for dogs and cats according to claim 1, characterized by that: the fatty acid comprises the following components in parts by weight: 35-55 parts of fish oil and 25-45 parts of chicken oil.
4. The creotropic cream for dogs and cats according to claim 1, characterized by that: the vitamin and mineral complex comprises the following components in parts by weight: 1-3.5 parts of vitamin A, 0.5-1 part of vitamin D, 4-8 parts of vitamin E, 10.3-0.7 part of vitamin B, 20.5-1 part of vitamin B, 60.1-0.25 part of vitamin B, 1-3 parts of nicotinic acid, 0.7-1.3 parts of pantothenic acid, 120.3-0.5 part of vitamin B, 0.04-0.055 part of folic acid, 0.001-0.0012 part of biotin, 12-17 parts of choline chloride, 74-87 parts of milk calcium, 7-12 parts of magnesium sulfate, 8-13 parts of ferrous lactate, 8-12 parts of zinc gluconate and 7-13 parts of selenium-enriched yeast (2000 ppm/kg).
5. The creotropic cream for dogs and cats according to claim 1, characterized by that: the components of the matrix in parts by weight comprise: 55-65 parts of concentrated whey protein, 55-70 parts of goat milk powder, 25-35 parts of chicken powder, 25-40 parts of chicken liver powder and 25-40 parts of crucian meat powder.
6. The creotropic cream for dogs and cats according to claim 1, characterized by that: the components of the forming agent in parts by weight comprise: 2-3.5 parts of edible gelatin and 12-18.5 parts of sodium carboxymethylcellulose.
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