CN111467447A - Chinese herbal medicine composition for inhibiting skin allergy - Google Patents

Abstract

The invention discloses a Chinese herbal medicine composition for inhibiting skin allergy, which contains honeysuckle, salvia miltiorrhiza, acacia flower, peony root bark, red paeony root, scutellaria baicalensis, medicinal rhubarb, glabrous greenbrier rhizome and liquorice; the solvent is octyl glycol, ethylhexyl glycerol, 1, 3-propylene glycol; the extract of the composition contains vitamin A, vitamin B, vitamin C, various inorganic salts such as potassium nitrate, potassium chloride, potassium sulfide and the like, and also contains oxalic acid, resin, flavonoid, norepinephrine and the like. It has effects in relieving skin, inhibiting skin pruritus due to dryness, preventing skin dryness and aging, improving skin comfort, scavenging free radicals, and improving skin quality.

Description

Chinese herbal medicine composition for inhibiting skin allergy

Technical Field

The invention relates to a Chinese herbal medicine composition for inhibiting skin allergy, in particular to a preparation method and application of an extract of the Chinese herbal medicine composition for inhibiting skin allergy.

Background

When the cosmetic is improperly used, phenomena such as skin allergy, pruritus, desquamation, red swelling, red blood streak and the like can be caused. Common allergens of cosmetic contact dermatitis are mainly preservatives (about 30-40% of cosmetic dermatitis allergens are preservatives), perfume essences (about 20-30%), surfactants (about 15%), sunscreens, antioxidants, antibacterial agents and the like. These components are all necessary additives in cosmetics. Skin sensitivity is increasingly receiving attention. In recent years, the cosmetic industry is gradually developing towards the direction of advocating green and returning to nature. The development of natural anti-allergy active substances with unique effects and the addition of the natural anti-allergy active substances into cosmetics to develop natural anti-allergy cosmetics become one of the most active subjects for the development of the cosmetic industry.

Nowadays, a lot of natural anti-allergy plant additives are applied to cosmetics, and are mainly divided into two categories, namely, one category is that natural plants are taken as raw materials to extract effective components and are applied to the cosmetics; the other is that the vegetable oil extracted from plant seeds, pulp and other parts is used as raw material to be added into cosmetics.

In the prior art:

the honeysuckle extract has complex chemical components and mainly contains chlorogenic acid, flavonoid, saponins and the like. The triterpenoid saponins, namely the lonicera glycoside A and the lonicera glycoside C, have anti-inflammatory effects which are equivalent to aspirin, and in addition, the volatile oil in the honeysuckle has obvious anti-inflammatory activity.

The water soluble extract of red sage root has unique and pleasant natural fragrance, is an ideal natural raw material for beauty and skin care products, can fully moisten and nourish the skin by promoting the microcirculation of the body surface, and can make the human face ruddy and glossy, eliminate ecchymosis and delay senility.

The research shows that the main chemical components of the sophora flower comprise flavone and glycosides, saponin and aglycone thereof, steroids, wax, green pigment, resin, pigment, protein (or mucus), amino acid (or peptide), condensed tannin and sugar, lauric acid, dodecenoic acid, myristic acid, tetradecenoic acid, palmitic acid, hexadecenoic acid, stearic acid and other fatty acids, β -sitosterol, D-glucose, glucohexa-glucuronic acid and gluconic acid propyl ester, vitamin A substances and the like, wherein the flavonoid glycoside (namely rutin) and the quercetin thereof have the effects of resisting inflammation, resisting viruses, resisting fungi, reducing blood fat, inhibiting aldose reductase and the like, the contained genistein has an anti-tumor effect on a human body, particularly the contained main component flavonoid glycoside has vitamin P-like effect, can reduce abnormal permeability and fragility of capillaries, can be used for treating and preventing diseases such as hypertension, cerebral hemorrhage, bleeding and the like, can maintain vascular resistance and the like, and the contained medical components such as the quercetin and the saponin have the effects of reducing blood pressure, enhancing vascular flow, dilating coronary arteries, increasing coronary artery resistance, improving the medical science and the effects on the cardiovascular system and the aspects of modern hemostasis and the like.

Peony root has anti-inflammatory and antiallergic effects. Can prevent melanin generation and freckle. And can be used for resisting skin aging caused by peroxidation.

The radix paeoniae rubrathe contains 5 mg.kg-1. d-1 of total paeoniflorin, which has obvious inhibiting effect on excessive swelling of rat paw induced by carrageenan, and has obvious prevention and treatment effect on rat polyarthritis, the action mechanism of the radix paeoniae rubrathe can be related to the synthesis of local inflammation factors such as PGE and leukotriene (L TB) by the total paeoniflorin, the inhibiting effect of the total paeoniflorin on NO secretion by mononuclear macrophage can be related to the anti-inflammatory mechanism of the total paeoniflorin.

The scutellaria baicalensis has obvious inhibition effect on mice delayed allergic reaction ear swelling caused by 2, 4-dinitrochlorobenzene, guinea pig skin vascular inflammation reaction and mice homologous passive skin anaphylaxis reaction. The results show that the scutellaria has certain treatment effect on the type I, II and IV anaphylactic reactions. Meanwhile, the medicine also has obvious inhibition effect on mouse itching reaction caused by low molecular dextran and guinea pig foot itching reaction caused by histamine, and shows that the scutellaria baicalensis has inhibition effect on itching symptoms. The literature also reports that the scutellaria baicalensis can inhibit the shrinkage of the guinea pig isolated ileum caused by histamine, and the result shows that the medicine has the function of antagonizing histamine and has obvious antiallergic effect. In addition, the scutellaria baicalensis can also inhibit the mouse ear swelling reaction caused by the dimethylbenzene, and the medicine has a certain anti-inflammatory effect.

The rhubarb is a common Chinese medicinal material, and has prominent efficacy and effect. The radix et rhizoma Rhei has strong anti-infection, antiaging, antioxidant, immunity regulating, antiinflammatory, antipyretic, and pathogenic microorganism resisting effects. Radix et rhizoma Rhei contains anthracene derivatives, glycosides, tannin, organic acids, volatile oil, etc., and can inhibit skin inflammatory reaction.

Poria mainly contains 2 kinds of compounds of triterpenes and polysaccharides; also contains steroids, choline, amino acid, histidine, volatile oil and trace elements represented by potassium salt, and has the main effects of promoting diuresis, excreting dampness, strengthening spleen and stomach, calming heart and tranquilizing mind and the like. The tuckahoe can resist acute and chronic inflammation under different experimental models, and the remarkable anti-inflammatory effect is highly recognized in foreign countries. Experiments show that the tuckahoe in the radix stephaniae tetrandrae and tuckahoe soup has a certain inhibition effect on activating the nitrite content in macrophages, and the high-dose ethanol extract of the tuckahoe has a certain cytotoxicity on resting macrophages. The pachyman can inhibit mice ear swelling caused by dimethylbenzene under a small dose, and simultaneously has an inhibition effect on the formation of rat subcutaneous granuloma caused by cotton balls, thereby proving that the pachyman has the effect of inhibiting acute and chronic inflammatory reactions.

Research on proliferation of muscle cells, proliferation promotion of vascular endothelial cells, collagen-4 type generation promotion, NO generation inhibition and the like of the liquorice extract shows that the liquorice extract has promotion effect on improving activity of skin and the like, and can be used for anti-aging cosmetics; the licorice extract has the effects of inhibiting melanin and whitening skin; has obvious inhibiting effect on some bacteria, and can be used as anti-inflammatory agent.

Disclosure of Invention

The invention adopts a technical scheme for solving the technical problems and provides a Chinese herbal medicine composition for inhibiting skin allergy, wherein the specific technical scheme is as follows:

comprises flos Lonicerae, Saviae Miltiorrhizae radix, flos Sophorae Immaturus, cortex moutan, radix Paeoniae Rubra, Scutellariae radix, radix et rhizoma Rhei, rhizoma Smilacis Glabrae, and Glycyrrhrizae radix; the solvent includes octyl glycol, ethylhexyl glycerin, and 1, 3-propylene glycol.

The Chinese herbal medicine composition for inhibiting skin allergy comprises the following components in percentage by mass: 5-30% of honeysuckle, 5-30% of salvia miltiorrhiza, 5-20% of acacia flower, 5-30% of peony root bark, 5-20% of red peony root, 5-30% of scutellaria baicalensis, 5-30% of medicinal rhubarb, 10-20% of glabrous greenbrier rhizome and 5-20% of liquorice.

A method for extracting and preparing a Chinese herbal medicine composition for inhibiting skin allergy comprises the following steps:

washing, drying and pulverizing the composition;

the extraction solvent is 75% ethanol;

the extraction process comprises leaching, extracting to obtain extract, adding octyl glycol, ethylhexyl glycerol, and 1.3-propylene glycol, and dissolving.

The extraction and preparation method of the Chinese herbal medicine composition for inhibiting skin allergy is characterized in that the extraction method is a two-time extraction method;

primary leaching: adding the mixture into an extraction tank for leaching for 2 hours at the temperature of 50-60 ℃ when the ratio of material to liquid is 1: 5;

secondary leaching: taking out the primary extraction filtrate, adding 75% ethanol into the filter cake according to the material-liquid ratio of 1:3 for secondary extraction, and extracting for 2 hours at the temperature of 50-60 ℃;

mixing the two filtrates, performing plate filtration, and settling to remove impurities;

evaporating the obtained extract to obtain extract;

the dissolving solvent is octyl glycol, ethylhexyl glycerol and 1, 3-propylene glycol in a ratio of 2:3: 5;

and uniformly dissolving the extract and the solvent according to the ratio of 3: 7.

An application of Chinese herbal medicine composition for inhibiting skin allergy is applied to preparing soothing skin care products, including facial masks, water, essence, skin moistening cream, cream and washing products, and the adding amount is 0.5-3%.

Compared with the prior art, the invention has the following beneficial effects:

the extract of the composition contains vitamin A, vitamin B, vitamin C, various inorganic salts such as potassium nitrate, potassium chloride, potassium sulfide and the like, and also contains oxalic acid, resin, flavonoid, norepinephrine and the like. It has effects in relieving skin, inhibiting skin pruritus due to dryness, preventing skin dryness and aging, improving skin comfort, scavenging free radicals, and improving skin quality.

The honeysuckle, the salvia miltiorrhiza and the medicinal rhubarb contain rich protein, mineral substances, cellulose, vitamin C and the like, and also contain rich active ingredients such as superoxide dismutase (SOD), flavonoid, polysaccharide and the like, so that the honeysuckle, the salvia miltiorrhiza and the medicinal rhubarb have the effects of protecting skin, beautifying, preventing sun, resisting aging, resisting allergy, diminishing inflammation, resisting cancer and enhancing the immunity of organisms.

The biochemical experiment proves that: the composition can inhibit hyaluronidase activity, and has good effect in eliminating DPPH and free radicals.

Detailed Description

The present invention will be further described with reference to the following examples.

Effect verification experiment 1 Hyaluronidase inhibition experiment

30% DMSO and hyaluronidase (1200 units. ml-1, 5ml) were added to 0.1 mol. l-1 acetate buffer (pH4.0), 2ml each of glutathione solutions with different mass concentrations were added, respectively, and incubated at 37 ℃ for 20min, after adding a mixture of compound 48/80CaCl2 and NaCl (1 ml each) and incubating at 37 ℃ for 20min, hyaluronic acid (1.2 mg. ml-1, 5ml) was added, the mixture was incubated at 37 ℃ for 40min, and then the reaction was stopped by adding 0.4 mol. l-1NaOH (4ml) and cooling on ice, after 10min, 800 mmol. l-1 sodium borate buffer (pH4.0, 3ml) was added, the mixture was incubated at 100 ℃ for 3min, and cooled on ice, 15ml of a developer (1g of p-dimethylbenzaldehyde dissolved in 100ml of acetic acid solution containing 1.25ml of concentrated HC L) was added, and then the mixture was incubated at 37 ℃ for 2min, absorbance at 540nm, and the hyaluronidase inhibition activity was calculated as in Table 1):

TABLE 1 Effect of Combined extracts on Hyaluronidase Activity

Effect verification experiment 2 DPPH-free radical scavenging experiment

Adding a DPPH solution and a sample solution into a 96-well plate according to a volume ratio of 1:1, shaking for 30s, and observing the absorbance (OD value) measured at 517nm after the dark reaction time is 15 min, 30 min and 45min respectively, wherein the absorbance of the sample solution added into the DPPH solution is A1(100 mu L DPPH solution +100 mu L sample solution), the absorbance of the sample solution is A2(100 mu L absolute ethyl alcohol +100 mu L sample solution), the absorbance of the DPPH solution added into a blank solvent is A3(100 mu L DPPH solution +100 mu L absolute ethyl alcohol), and the DPPH clearance (%) is calculated according to a formula, the DPPH free radical removal absorbance of the sample solution is A3- (A1-A2), and the DPPH clearance/% (1- (A1-A2)/A3) × 100%, and the detection results are shown in Table 2:

TABLE 2 DPPH & free radical scavenging ability of the combined extracts

Example 1A herbal composition for skin allergy inhibition

Washing flos Lonicerae, Saviae Miltiorrhizae radix, flos Sophorae Immaturus, cortex moutan, radix Paeoniae Rubra, Scutellariae radix, radix et rhizoma Rhei, rhizoma Smilacis Glabrae, and Glycyrrhrizae radix, drying in blast drying oven.

Weighing 5Kg of dried honeysuckle, 5Kg of salvia miltiorrhiza, 5Kg of acacia flower, 5Kg of peony root bark, 5Kg of red paeony root, 5Kg of baical skullcap root, 5Kg of medicinal rhubarb, 15Kg of glabrous greenbrier rhizome and 5Kg of liquoric root.

Placing the pulverized Chinese herbal medicines into a constant-temperature extraction tank.

Primary leaching: adding 75% ethanol 300KG, and leaching in extraction tank at 50-60 deg.C for 2 hr.

Secondary leaching: taking out the primary extraction filtrate, adding 180kg of 75% ethanol into the filter cake according to the feed liquid, and leaching for 2 hours at the temperature of 50-60 ℃.

And combining the two filtrates, performing plate filtration, and settling to remove impurities.

Evaporating the obtained extractive solution to obtain extract 4.5 kg.

The dissolving solvent is octyl glycol, ethylhexyl glycerol and 1, 3-propylene glycol in a ratio of 2:3: 5.

Adding the extract and a solvent according to the proportion of 3: 7: 10.5Kg of the product is dissolved evenly to obtain the required product.

Example 2 use of a herbal composition for skin allergy inhibition in a facial mask

The components and contents (%) of the mask are as follows:

a Chinese herbal composition for inhibiting skin allergy comprises: 1

Water: TO100

Glycerol: 3

EDTA：0.05

Sodium hyaluronate: 0.05

Xanthan gum: 0.1

Carbomer: 0.15

Betaine: 1.5

Arginine: 0.12

The preparation method of the facial mask comprises the following steps:

94.33Kg of water, 3Kg of glycerin, 0.05Kg of EDTA, 0.05Kg of sodium hyaluronate, 0.1Kg of xanthan gum, 0.15Kg of carbomer and 1.5Kg of betaine are put into a water pot, heated to 85 ℃, stirred for 30 minutes, homogenized for 3 minutes and uniformly dispersed.

Cooling to 40 ℃, adding 0.12Kg of arginine, and stirring uniformly.

Cooling to room temperature and adding a Chinese herbal composition for inhibiting skin allergy: 1Kg, and stirring the mixture evenly.

The prepared facial mask essence can become a natural relieving facial mask for daily use by people by adding the mask cloth.

Example 3 use of a herbal composition for skin allergy inhibition in cream

The cream comprises the following components in percentage by weight:

a Chinese herbal composition for inhibiting skin allergy comprises: 3

Water: TO100

Glycerol: 3

EDTA：0.05

Sodium hyaluronate: 0.05

Acrylamide dimethyl ammonium taurate/VP copolymer: 1

Betaine: 1.5

Cetearyl glucoside, sorbitan olivate, cetearyl alcohol: 1.5

Caprylic/capric triglyceride: 8

The preparation method of the cream comprises the following steps:

82.9Kg of water, 3Kg of glycerin, 0.05Kg of EDTA, 0.05Kg of sodium hyaluronate, 1Kg of acrylamide dimethyl ammonium taurate/VP copolymer and 1.5Kg of betaine are put into an emulsifying pot, heated to 85 ℃, stirred for 30 minutes, homogenized for 3 minutes and uniformly dispersed.

Adding cetearyl glucoside, sorbitan olivate, cetearyl alcohol 1.5Kg, caprylic/capric triglyceride 8Kg into oil pan, and dissolving at 85 deg.C.

Cooling to room temperature, adding 3Kg of a Chinese herbal medicine composition for inhibiting skin allergy, and stirring uniformly.

The preparation of the natural soothing cream is completed.

Claims (5)

1. A Chinese herbal composition for inhibiting skin allergy is characterized by comprising flos Lonicerae, Saviae Miltiorrhizae radix, flos Sophorae Immaturus, cortex moutan, radix Paeoniae Rubra, Scutellariae radix, radix et rhizoma Rhei, rhizoma Smilacis Glabrae, and Glycyrrhrizae radix; the solvent includes octyl glycol, ethylhexyl glycerin, and 1, 3-propylene glycol.

2. The herbal skin allergy-inhibiting composition of claim 1, consisting of, in mass percent: 5-30% of honeysuckle, 5-30% of salvia miltiorrhiza, 5-20% of acacia flower, 5-30% of peony root bark, 5-20% of red peony root, 5-30% of scutellaria baicalensis, 5-30% of medicinal rhubarb, 10-20% of glabrous greenbrier rhizome and 5-20% of liquorice.

3. A method for preparing a herbal composition for skin allergy suppression as claimed in any one of claims 1 and 2, comprising the steps of:

washing, drying and pulverizing the composition;

the extraction solvent is 75% ethanol;

the extraction process comprises leaching, extracting to obtain extract, adding octyl glycol, ethylhexyl glycerol, and 1.3-propylene glycol, and dissolving.

4. The method for preparing a herbal composition for skin allergy suppression as claimed in claim 3, wherein the extraction is characterized by two extractions;

primary leaching: adding the mixture into an extraction tank for leaching for 2 hours at the temperature of 50-60 ℃ when the ratio of material to liquid is 1: 5;

secondary leaching: taking out the primary extraction filtrate, adding 75% ethanol into the filter cake according to the material-liquid ratio of 1:3 for secondary extraction, and extracting for 2 hours at the temperature of 50-60 ℃;

mixing the two filtrates, performing plate filtration, and settling to remove impurities;

evaporating the obtained extract to obtain extract;

the dissolving solvent is octyl glycol, ethylhexyl glycerol and 1, 3-propylene glycol in a ratio of 2:3: 5;

and uniformly dissolving the extract and the solvent according to the ratio of 3: 7.

5. Use of a herbal composition for skin allergy suppression according to claim 4 in the preparation of soothing skin care products including facial masks, water, essences, skin lotions, creams and washing products in an amount of 0.5-3%.