CN106420531B - It is a kind of containing hiding schizonepeta have effects that the Chinese traditional medicine composition extract of skin whitening, moisturizing and application - Google Patents
It is a kind of containing hiding schizonepeta have effects that the Chinese traditional medicine composition extract of skin whitening, moisturizing and application Download PDFInfo
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- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
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- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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Abstract
The invention discloses a kind of schizonepeta containing hiding to have effects that the Chinese traditional medicine composition extract of skin whitening, moisturizing, is prepared by the combined extracts by following Chinese medicine materials by weight: hiding schizonepeta 10-30 parts, 10-40 parts of black fruit fructus lycii leaf, 10-30 parts of Malus toringoides Hughes leaf, 10-40 parts of highland barley, 10-30 parts of rhizoma polygonati.The Chinese traditional medicine composition extract of the schizonepeta containing hiding of the invention has the characteristics that skin whitening, moisturizing, natural safety, synergy.
Description
Technical field
The invention belongs to fine chemistry industry technical field of daily necessities, and in particular to be that a kind of schizonepeta containing hiding has skin whitening, moisturizing
The Chinese traditional medicine composition extract of effect and application.
Background technique
The colour of skin refers to the skin that human skin surface layer is reflected by pigment depositions such as melanin, hemn, phylloerythrin
Color.Wherein, melanin plays a decisive role.The effect of script melanin is radiation injury of the ultraviolet light to skin in order to prevent.
Once it is superfluous that melanin secretion occurs in skin, just will appear spot deposition, thus lead to the problem of it is a series of cosmetically.
Cause there are many kinds of melanin Producing reasons, such as ultraviolet light exhaust gas, active oxygen environmental problem and certain heredity
Factor, Producing reason is considerably complicated, and universal result of study shows that the process of B16 cell is as follows, with intracellular junket ammonia
Acid is used as matrix, and DOPA quinone is generated under the action of tyrosinase, becomes to be further oxidized to after dopachrome from DOPA quinone
5,6- dihydroxy indoles and 5,6- dihydroxy -2- hydroxy acid, the polymerization of these products ultimately generate aggressiveness melanin.In cell metabolism
Promotion layer by layer under be transferred to skin surface, excreted if cannot be metabolized in time, precipitating will be built up and form color
Spot eventually leads to hyperchromlsm.And the reason of leading to dry skin, has very much, mainly skin moisture deficiency, grease are secreted not
Caused by balance and collagen are lost etc., show as skin-bound, individual sites it is dry itch, skin color dimness etc.
Deng, and have dry skin and be also unfavorable for further skin care.
At present in research, skin-whitening agents are usually all using tyrosinase as action target spot, by inhibiting tyrosinase
Activity or block oxidizing tyrosine generate melanin reaction to reduce the generation of dermal melanin, thus reach skin beauty
White effect.It is most of using hydrogen peroxide, ammoniated mercury and various phenol derivatives as skin-whitening agents, these
Compound can rapidly disintegrate melanin tissue and achieve the effect that quick whitening, but have corrosivity, cytotoxicity to skin
It is poor with adverse effects, the safety such as anaphylaxis.In addition, the whitening agent inhibiting mechanism of one-component is relatively simple, cannot reach
Ideal whitening effect.For skin moisture-keeping, physical method moisturizing or the stage using moisture saver mask are rested on mostly at present,
These products are capable of providing certain moisture-keeping function, but moisture preserving time is shorter, cannot be guaranteed in terms of safety, and to skin
Skin also has certain stimulation.
Currently, on the market there are many kinds of skin whitening, moisturizing products, but mostly all containing the huge sum of money such as lead, mercury in some whitening products
Belong to substance.In the present invention, a variety of natural extracts are applied in combination, not only can satisfy the effect of skin whitening, moisturizing, but also can guarantee
Skin is not stimulated, no tingling sensation.
Hiding schizonepeta is the herb that Lamiaceae plant hides schizonepeta Nepeta angustifolia C.Y.Wu.It originates in Tibet
(Jiangzi, Lhasa) is the distinctive Tibetan medicine material in Tibet.Hiding schizonepeta be clinically mainly used for coma convulsion faint, apoplexy, epilepsy, brain overflow
Blood, sore injure the treatment of the diseases such as pain, have good curative effect." Jingzhubencao " is pointed out: this product " controls epilepsy, cerebral hemorrhage, sore wound
And the diseases such as pain ", also there are " Tibetan medicine will ", " medicine name is assembled " hiding schizonepeta treatment apoplexy, evil disease, pleurisy and dermopathic record.
Modern studies have found that the effects of hiding schizonepeta can play swelling and pain relieving, wound healing.In addition, some groups of hiding Herba Schizonepetae volatile oil
Dividing has anti-oxidant, anti-inflammatory, analgesic effect.
Malus toringoides Hughes leaf is also referred to as E Seye, is the medicinal material of the civil dual-purpose of drug and food of Tibetan Autonomous Prefecture of Garze Tibetan, derives from
The dried leaf of rosaceous plant Malus toringoides Hughes Malus toringoides (Rehd.) Hughes.It is recorded in " Tibetan medicine crystalline substance mirror earliest
Book on Chinese herbal medicine " and " hiding Chinese voluminous dictionary ", it is recorded according to this two medical books, which has assaulting fortified position product, except function such as the stagnant, Hepatoprotective cholagogues of greasy paint
Effect, its magical medicinal efficacy have been logged the most authoritative Tibetanmedicine works Four-Volume Medical Code.For making for Malus toringoides Hughes leaf
With traditional Tibetan medicine's usage is oral with boiling with butter.It is mainly used for treating indigestion and some liver diseases, it is curative for effect,
Usage is unique.Modern pharmacological studies have shown that contain higher Flavonoids in Malus toringoides Hughes leaf, to DPPH free radical and
Hydroxyl radical free radical has preferable scavenging capacity, and chemical component phloridzin, phloretin have anti-atherogenic in Malus toringoides Hughes leaf
The pharmacological actions such as hardening, anti-inflammatory.
Black fruit fructus lycii leaf is the cauline leaf of Solanaceae Lycium black fruit fructus lycii Lycium Ruthenicum Murr..Currently,
The research of black fruit fructus lycii focus mostly on the pigment contained by its fruit and polysaccharide extraction and bioactivity in terms of, and its blade is extracted
Object has not been reported beautifying whitening.Modern studies have found that general flavone is to removing mice serum hydroxyl radical free radical in black fruit fructus lycii leaf
Influence, to the protective effect of erythrocyte hemolysis and inhibit mouse liver MDA to generate aspect all there is remarkable efficacy, show
Apparent antioxidant activity.Researcher of the present invention has found that lycium ruthenicum leaf extract has inhibiting effect to tyrosinase, shows
Certain white-skinned face function.
Highland barley Hordeum vulgareLinn.var.nudum Hook.f., that is, naked barley is grass family Hordeum crop,
It is Qinghai-Tibet that Tibet, Qinghai, the Ganzi in Sichuan and Aba Prefecture, the enlightening celebrating in Yunnan, the Gannan etc. in Gansu are mainly distributed in China
Area is exactly one of the staple food crop of the Tibetan area people since ancient times.It is poly- containing great amount of soluble fiber, β-Portugal in highland barley
The nutritional ingredients such as sugar, vitamin, minerals.Highland barley pigment content in highland barley is higher.Modern studies have found that highland barley pigment has
It is anti-oxidant, adjust blood lipid, prevent coronary heart disease, the anti-inflammatory and pharmacological actions such as antitumor, it is wide to can also be used as ultraviolet absorbing agent
It is general to be applied to cosmetic industry.It is black big that animal experiment study shows that highland barley pigment is higher than the scavenging capacity of hydroxyl radical free radical
Other natural pigments such as beans, Radix Ophiopogonis；Mice serum antiactive oxygen ability can be enhanced, peroxide MDA is inhibited to generate, it is significant to drop
MDA content in low each organ-tissue.
Rhizoma polygonati Polygonatum sibiricum is Liliaceae Polygonatum herbaceos perennial, and smell is gentle, and quality is grown
Profit has the yin of tonic lung, moisturizes the effect of promoting the production of body fluid.Siberian solomonseal rhizome polysaccharide is principle active component and the rhizoma polygonati master of rhizoma polygonati water extract
Want one of biological active ingredients.Modern pharmacology confirms that Siberian solomonseal rhizome polysaccharide has hypoglycemic, reducing blood lipid, enhancing immune function, antioxygen
The functions such as change, anti-aging.The removing of interior free yl depends on the activity of the antioxidases such as SOD, GSH-Px, and MDA is as body
The product of peroxidatic reaction of lipid can reflect body cell by the degree of free radical attack damage.Animal experiment study shows
Siberian solomonseal rhizome polysaccharide can be substantially reduced rat blood serum, skeletal muscle MDA content, improve the activity of rat blood serum endogenous SOD and GSH-Px
And skeletal muscle endogenous SOD activity, illustrate that Siberian solomonseal rhizome polysaccharide has stronger anti-oxidant, anti-aging effects.
Summary of the invention
The purpose of the present invention is overcome the deficiencies of the prior art and provide one kind schizonepeta containing hiding to pacify with skin whitening, moisturizing, naturally
Entirely, synergistic Chinese traditional medicine composition extract and application.
In order to achieve the above-mentioned object of the invention, technical scheme is as follows:
A kind of schizonepeta containing hiding has effects that the Chinese traditional medicine composition extract of skin whitening, moisturizing, the combined extracts by it is following by weight
The Chinese medicine material of part meter is prepared: hiding schizonepeta 10-30 parts, 10-40 parts of black fruit fructus lycii leaf, 10-30 parts of Malus toringoides Hughes leaf, highland barley
10-40 parts, 10-30 parts of rhizoma polygonati.
Preferably, which is prepared by following Chinese medicine materials according to the mass fraction: hiding schizonepeta 10-
20 parts, 20-40 parts of black fruit fructus lycii leaf, 10-20 parts of Malus toringoides Hughes leaf, 20-40 parts of highland barley, 10-20 parts of rhizoma polygonati.
The schizonepeta containing hiding has effects that the preparation method of the Chinese traditional medicine composition extract of skin whitening, moisturizing, comprising the following steps:
(1) dry hiding schizonepeta, black fruit fructus lycii leaf, Malus toringoides Hughes leaf medicinal material are taken, is crushed, 20 meshes are crossed, according to volume matter
Amount is extracted 1-3 hours every time than 5-8 times of amount (V/m) 50%-80% ethyl alcohol, refluxing extraction 1-3 times is added, and filtration, filtrate is closed
And ethyl alcohol is recovered under reduced pressure under the conditions of 55-75 DEG C to no alcohol taste, then upper D101 type or AB-8 type macroreticular resin column purification, it collects
40%-70% ethanol elution part is recovered under reduced pressure ethyl alcohol under the conditions of 60 DEG C, obtains medicinal extract, be dried in vacuo under the conditions of 50-70 DEG C, powder
It is broken to get above-mentioned three tastes medicinal substances extract.
(2) dry highland barley, rhizoma polygonati are taken, is crushed, 40 meshes are crossed, 10-20 times is added according to mass ratio and measures water, unsaturated carbonate
It is 8.0 that sodium solution, which adjusts pH value, and heating and refluxing extraction 1-3 hours, extracting solution 3000-5000rpm/min was centrifuged 15-30min, is taken
Supernatant, adjusting pH value with the hydrochloric acid solution of 1mol/L is 6.0, and Thermostable α-Amylase enzymatic hydrolysis is added by highland barley inventory 5u/g
Starch, 85-95 DEG C enzymatic hydrolysis 30-45 minutes, checked with iodine solution complete to enzymatic hydrolysis.It boils enzyme deactivation 10 minutes, lets cool to room temperature,
3000-5000rpm/min is centrifuged 15-30min, takes supernatant, and adjusting pH value with the hydrochloric acid solution of 1mol/L is 4.5, places
Night.3000-5000rpm/min is centrifuged 15-30min, takes supernatant, and adjusting pH value with saturated sodium carbonate solution is 7.0, is concentrated into
Solid-liquid ratio 1:3 (m:V), adding 95% ethyl alcohol to alcohol content is 70%, and stirring is stood overnight.It filters, must precipitate, it is dry, it is as green
Highland barley, Rhizoma Polygonati extract.
(3) highland barley that the extract that step (1) obtains is obtained with step (2), Rhizoma Polygonati extract are uniformly mixed, as this
The schizonepeta containing hiding of invention has effects that the Chinese traditional medicine composition extract of skin whitening, moisturizing.
A kind of above-mentioned schizonepeta containing hiding has effects that the Chinese traditional medicine composition extract of skin whitening, moisturizing is preparing answering in skin care item
With.
Mass percentage of the Chinese traditional medicine composition extract for having effects that skin whitening, moisturizing in skin whitening, moisturizing skin care item
For 0.1-10%, preferred mass percentage composition is 1-8%, remaining is the common matrix and deionized water of cosmetics exterior-applied formulation
Or purified water or distilled water.By common process, it can be made into whitening and moisturizing facial cleanser and preparation method of facial cleanser, skin whitening, moisturizing Cleansing Foam, skin whitening, moisturizing and wash
Face cream, skin whitening, moisturizing cream, skin whitening, moisturizing frost, skin whitening, moisturizing lotion, skin whitening, moisturizing water, whitening moisturizing face masque, skin whitening, moisturizing gel
Or the cosmetics exterior-applied formulation such as skin whitening, moisturizing soap.
Solution of the invention is based on relevant medical tradition theory, in conjunction with modern medicine and pharmacology investigative technique and hand
Section, by many experiments preferably with verifying, it was found that hiding schizonepeta, black fruit fructus lycii leaf, Malus toringoides Hughes leaf, highland barley, the five tastes such as rhizoma polygonati
Medicinal material is used cooperatively, and can play apparent skin whitening, moisturizing skin care effect.Formula preferred for this invention, is examined by domestic and foreign literature
Rope does not find related application, is screened in addition by many experiments, and gained formula rate is optimum formula ratio, and formula is scientific, and
It is highly-safe, it can be used for daily skin nursing.
The invention has the following advantages that
(1) skin whitening, moisturizing for having found that hiding schizonepeta, black fruit fructus lycii leaf, Malus toringoides Hughes leaf, highland barley, rhizoma polygonati are used cooperatively is made
With expanding the application range of above-mentioned raw materials, application especially in the treatment of skin；
(2) it screens through a large number of experiments, it is determined that the optimal proportion range of raw material, it is ensured that the effect that product of the present invention uses
Fruit；
(3) inventive formulation definite effect and stabilization, it is unstable effectively to overcome natural products skin care item effect, also overcomes
The disadvantages of pure chemistry is at partial safety factor lower.
Specific embodiment
Technical solution of the invention is further illustrated combined with specific embodiments below, these embodiments should not be understood as
It is the limitation to technical solution.
Embodiment 1
Hide 10 parts of schizonepeta, 40 parts of black fruit fructus lycii leaf, 10 parts of Malus toringoides Hughes leaf, 30 parts of highland barley, 10 parts of rhizoma polygonati
(1) dry hiding schizonepeta, black fruit fructus lycii leaf, Malus toringoides Hughes leaf medicinal material are taken, is crushed, 20 meshes are crossed, according to volume matter
Amount refluxing extraction 3 times, is extracted 2 hours, filtration, filtrate merges, in 65 DEG C of conditions every time than 8 times of amount (V/m) 70% ethyl alcohol are added
Under ethyl alcohol is recovered under reduced pressure to no alcohol taste, then upper AB-8 type macroreticular resin column purification collects 60% ethanol elution part, 60 DEG C of conditions
Under ethyl alcohol is recovered under reduced pressure, obtain medicinal extract, be dried in vacuo under the conditions of 70 DEG C, crush to get above-mentioned three tastes medicinal substances extract.
(2) dry highland barley, rhizoma polygonati are taken, is crushed, 40 meshes are crossed, 20 times of amount water, saturated sodium carbonate is added according to mass ratio
It is 8.0 that solution, which adjusts pH value, and heating and refluxing extraction 3 hours, extracting solution 5000rpm/min was centrifuged 30min, took supernatant, is used
It is 6.0 that the hydrochloric acid solution of 1mol/L, which adjusts pH value, by highland barley inventory 5u/g addition Thermostable α-Amylase enzymatic starch, 95 DEG C
Enzymatic hydrolysis 45 minutes is checked complete to enzymatic hydrolysis with iodine solution.It boils enzyme deactivation 10 minutes, lets cool to room temperature, 5000rpm/min centrifugation
30min takes supernatant, and adjusting pH value with the hydrochloric acid solution of 1mol/L is 4.5, stands overnight.5000rpm/min is centrifuged 30min,
Supernatant is taken, adjusting pH value with saturated sodium carbonate solution is 7.0, is concentrated into solid-liquid ratio 1:3 (m:V), adds 95% ethyl alcohol to alcohol-containing
Amount is 70%, and stirring is stood overnight.It filters, must precipitate, drying, as highland barley, Rhizoma Polygonati extract.
(3) highland barley that the extract that step (1) obtains is obtained with step (2), Rhizoma Polygonati extract are uniformly mixed, as this
The schizonepeta containing hiding of invention has effects that the Chinese traditional medicine composition extract of skin whitening, moisturizing.
Embodiment 2
Hide 20 parts of schizonepeta, 20 parts of black fruit fructus lycii leaf, 10 parts of Malus toringoides Hughes leaf, 40 parts of highland barley, 10 parts of rhizoma polygonati
(1) dry hiding schizonepeta, black fruit fructus lycii leaf, Malus toringoides Hughes leaf medicinal material are taken, is crushed, 20 meshes are crossed, according to volume matter
Amount refluxing extraction 2 times, is extracted 3 hours, filtration, filtrate merges, in 75 DEG C of conditions every time than 6 times of amount (V/m) 80% ethyl alcohol are added
Under ethyl alcohol is recovered under reduced pressure to no alcohol taste, then upper D101 type macroreticular resin column purification collects 70% ethanol elution part, 60 DEG C of conditions
Under ethyl alcohol is recovered under reduced pressure, obtain medicinal extract, be dried in vacuo under the conditions of 55 DEG C, crush to get above-mentioned three tastes medicinal substances extract.
(2) dry highland barley, rhizoma polygonati are taken, is crushed, 40 meshes are crossed, 15 times of amount water, saturated sodium carbonate is added according to mass ratio
It is 8.0 that solution, which adjusts pH value, and heating and refluxing extraction 2 hours, extracting solution 3000rpm/min was centrifuged 20min, took supernatant, is used
It is 6.0 that the hydrochloric acid solution of 1mol/L, which adjusts pH value, by highland barley inventory 5u/g addition Thermostable α-Amylase enzymatic starch, 90 DEG C
Enzymatic hydrolysis 30 minutes is checked complete to enzymatic hydrolysis with iodine solution.It boils enzyme deactivation 10 minutes, lets cool to room temperature, 3000rpm/min centrifugation
20min takes supernatant, and adjusting pH value with the hydrochloric acid solution of 1mol/L is 4.5, stands overnight.3000rpm/min is centrifuged 20min,
Supernatant is taken, adjusting pH value with saturated sodium carbonate solution is 7.0, is concentrated into solid-liquid ratio 1:3 (m:V), adds 95% ethyl alcohol to alcohol-containing
Amount is 70%, and stirring is stood overnight.It filters, must precipitate, drying, as highland barley, Rhizoma Polygonati extract.
(3) highland barley that the extract that step (1) obtains is obtained with step (2), Rhizoma Polygonati extract are uniformly mixed, as this
The schizonepeta containing hiding of invention has effects that the Chinese traditional medicine composition extract of skin whitening, moisturizing.
Embodiment 3
Hide 15 parts of schizonepeta, 25 parts of black fruit fructus lycii leaf, 10 parts of Malus toringoides Hughes leaf, 35 parts of highland barley, 15 parts of rhizoma polygonati
(1) dry hiding schizonepeta, black fruit fructus lycii leaf, Malus toringoides Hughes leaf medicinal material are taken, is crushed, 20 meshes are crossed, according to volume matter
Amount refluxing extraction 3 times, is extracted 2 hours, filtration, filtrate merges, in 55 DEG C of conditions every time than 7 times of amount (V/m) 80% ethyl alcohol are added
Under ethyl alcohol is recovered under reduced pressure to no alcohol taste, then upper AB8 type macroreticular resin column purification collects 50% ethanol elution part, 60 DEG C of conditions
Under ethyl alcohol is recovered under reduced pressure, obtain medicinal extract, be dried in vacuo under the conditions of 65 DEG C, crush to get above-mentioned three tastes medicinal substances extract.
(2) dry highland barley, rhizoma polygonati are taken, is crushed, 40 meshes are crossed, 10 times of amount water, saturated sodium carbonate is added according to mass ratio
It is 8.0 that solution, which adjusts pH value, and heating and refluxing extraction 3 hours, extracting solution 4500rpm/min was centrifuged 15min, took supernatant, is used
It is 6.0 that the hydrochloric acid solution of 1mol/L, which adjusts pH value, by highland barley inventory 5u/g addition Thermostable α-Amylase enzymatic starch, 95 DEG C
Enzymatic hydrolysis 45 minutes is checked complete to enzymatic hydrolysis with iodine solution.It boils enzyme deactivation 10 minutes, lets cool to room temperature, 4500rpm/min centrifugation
30min takes supernatant, and adjusting pH value with the hydrochloric acid solution of 1mol/L is 4.5, stands overnight.4500rpm/min is centrifuged 30min,
Supernatant is taken, adjusting pH value with saturated sodium carbonate solution is 7.0, is concentrated into solid-liquid ratio 1:3 (m:V), adds 95% ethyl alcohol to alcohol-containing
Amount is 70%, and stirring is stood overnight.It filters, must precipitate, drying, as highland barley, Rhizoma Polygonati extract.
(3) highland barley that the extract that step (1) obtains is obtained with step (2), Rhizoma Polygonati extract are uniformly mixed, as this
The schizonepeta containing hiding of invention has effects that the Chinese traditional medicine composition extract of skin whitening, moisturizing.
Embodiment 4
Hide 10 parts of schizonepeta, 30 parts of black fruit fructus lycii leaf, 20 parts of Malus toringoides Hughes leaf, 20 parts of highland barley, 20 parts of rhizoma polygonati
(1) dry hiding schizonepeta, black fruit fructus lycii leaf, Malus toringoides Hughes leaf medicinal material are taken, is crushed, 20 meshes are crossed, according to volume matter
Amount refluxing extraction 1 time, is extracted 3 hours, filtration, filtrate merges, in 60 DEG C of conditions every time than 5 times of amount (V/m) 80% ethyl alcohol are added
Under ethyl alcohol is recovered under reduced pressure to no alcohol taste, then upper D101 type macroreticular resin column purification collects 40% ethanol elution part, 60 DEG C of conditions
Under ethyl alcohol is recovered under reduced pressure, obtain medicinal extract, be dried in vacuo under the conditions of 50 DEG C, crush to get above-mentioned three tastes medicinal substances extract.
(2) dry highland barley, rhizoma polygonati are taken, is crushed, 40 meshes are crossed, 15 times of amount water, saturated sodium carbonate is added according to mass ratio
It is 8.0 that solution, which adjusts pH value, and heating and refluxing extraction 1 hour, extracting solution 3500rpm/min was centrifuged 15min, took supernatant, is used
It is 6.0 that the hydrochloric acid solution of 1mol/L, which adjusts pH value, by highland barley inventory 5u/g addition Thermostable α-Amylase enzymatic starch, 85 DEG C
Enzymatic hydrolysis 30 minutes is checked complete to enzymatic hydrolysis with iodine solution.It boils enzyme deactivation 10 minutes, lets cool to room temperature, 3500rpm/min centrifugation
20min takes supernatant, and adjusting pH value with the hydrochloric acid solution of 1mol/L is 4.5, stands overnight.3500rpm/min is centrifuged 20min,
Supernatant is taken, adjusting pH value with saturated sodium carbonate solution is 7.0, is concentrated into solid-liquid ratio 1:3 (m:V), adds 95% ethyl alcohol to alcohol-containing
Amount is 70%, and stirring is stood overnight.It filters, must precipitate, drying, as highland barley, Rhizoma Polygonati extract.
(3) highland barley that the extract that step (1) obtains is obtained with step (2), Rhizoma Polygonati extract are uniformly mixed, as this
The schizonepeta containing hiding of invention has effects that the Chinese traditional medicine composition extract of skin whitening, moisturizing.
Embodiment 5
Hide 10 parts of schizonepeta, 25 parts of black fruit fructus lycii leaf, 15 parts of Malus toringoides Hughes leaf, 35 parts of highland barley, 15 parts of rhizoma polygonati
(1) dry hiding schizonepeta, black fruit fructus lycii leaf, Malus toringoides Hughes leaf medicinal material are taken, is crushed, 20 meshes are crossed, according to volume matter
Amount refluxing extraction 1 time, is extracted 2 hours, filtration, filtrate merges, in 65 DEG C of conditions every time than 8 times of amount (V/m) 55% ethyl alcohol are added
Under ethyl alcohol is recovered under reduced pressure to no alcohol taste, then upper AB-8 type macroreticular resin column purification collects 55% ethanol elution part, 60 DEG C of conditions
Under ethyl alcohol is recovered under reduced pressure, obtain medicinal extract, be dried in vacuo under the conditions of 60 DEG C, crush to get above-mentioned three tastes medicinal substances extract.
(2) dry highland barley, rhizoma polygonati are taken, is crushed, 40 meshes are crossed, 10 times of amount water, saturated sodium carbonate is added according to mass ratio
It is 8.0 that solution, which adjusts pH value, and heating and refluxing extraction 1.5 hours, extracting solution 3000rpm/min was centrifuged 25min, took supernatant, is used
It is 6.0 that the hydrochloric acid solution of 1mol/L, which adjusts pH value, by highland barley inventory 5u/g addition Thermostable α-Amylase enzymatic starch, 90 DEG C
Enzymatic hydrolysis 40 minutes is checked complete to enzymatic hydrolysis with iodine solution.It boils enzyme deactivation 10 minutes, lets cool to room temperature, 4000rpm/min centrifugation
25min takes supernatant, and adjusting pH value with the hydrochloric acid solution of 1mol/L is 4.5, stands overnight.4000rpm/min is centrifuged 25min,
Supernatant is taken, adjusting pH value with saturated sodium carbonate solution is 7.0, is concentrated into solid-liquid ratio 1:3 (m:V), adds 95% ethyl alcohol to alcohol-containing
Amount is 70%, and stirring is stood overnight.It filters, must precipitate, drying, as highland barley, Rhizoma Polygonati extract.
(3) highland barley that the extract that step (1) obtains is obtained with step (2), Rhizoma Polygonati extract are uniformly mixed, as this
The schizonepeta containing hiding of invention has effects that the Chinese traditional medicine composition extract of skin whitening, moisturizing.
Embodiment 6
1 extract of embodiment, 2 extract of embodiment, 3 extract of embodiment test l-tyrosine inhibition of enzyme activity
1 materials and methods:
1.1 sample
1 extract of embodiment, 2 extract of embodiment, 3 extract of embodiment, arbutin (positive control).
1.2 experimental method
(1) preparation of phosphate buffer: precision weighs Na2HPO412H2O 26.797g, NaH2PO42H2O
13.803g is settled in 500mL volumetric flask respectively with after deionized water dissolving.Take sodium dihydrogen phosphate and disodium phosphate soln
Each 100mL is into 500mL volumetric flask, and deionized water constant volume shakes up, as the phosphate buffer of pH=6.8.
(2) above-mentioned four kinds of samples the preparation of sample solution: are configured to concentration 0.1mg/mL, 0.2mg/ with DMSO respectively
ML, 0.3mg/mL, 0.4mg/mL, 0.5mg/mL, 0.6mg/mL, 0.7mg/mL, 0.8mg/mL testing sample solution.
(3) preparation of substrate l-tyrosine solution: substrate l-tyrosine is configured to mass concentration with phosphate buffer and is
0.05% l-tyrosine solution.
(4) preparation of tyrosinase solution: tyrosinase is configured to the solution of 100U/mL with phosphate buffer solution.
(5) tetra- groups of C1, C2, T1, T2 for removing tyrosinase solution accurately the measurement of tyrosinase inhibition rate: are drawn by table 1
React sample liquid, 37 DEG C of constant temperature water baths after ten minutes, add tyrosinase solution, react 10 minutes, be the reference of C1 with C2,
System absorbance A 1 at 475nm is measured, is the reference of T1 with T2, measures system absorbance A 2.For parallel sample bring system
System error, has been separately added into the DMSO of ImL in C1 and C2.To eliminate sample light absorbance error at 475nm, also add in T2
LmL sample solution is entered.The inhibiting rate of tyrosinase is calculated according to the following formula, the results are shown in Table 2.
Wherein A1For be not loaded product reaction solution absorbance value change, A2For the absorbance of reaction solution after addition sample
The change of value.
1 tyrosinase inhibition test reagent dosage table of table
2 experimental results
Influence (%) of the extract of the present invention of table 2 to inhibitory activity against tyrosinase
The result shows that three kinds of embodiment extracts of the invention have good inhibiting effect to tyrosinase activity, wherein
The inhibitory effect of 1 extract of embodiment is best, and for concentration in 0.2mg/mL, inhibiting rate reaches 50.06%, and embodiment 2 is extracted
The concentration of object is in 0.4mg/mL, inhibiting rate 58.27%, and the concentration of 3 extract of embodiment inhibits in 0.4mg/mL
Rate is 52.14%, and with the increase of concentration, the inhibiting rate of tyrosinase also increase accordingly three kinds of Example formulations, wherein implements
The tyrosinase inhibition rate of example 1 is totally higher than embodiment 2 and embodiment 3.As a result illustrate three kinds of embodiment extracts to tyrosine
The inhibitory effect of enzyme is obvious, and three kinds of embodiment extracts all have good whitening effect, wherein especially with 1 extract of embodiment
Effect it is best, when concentration be 0.8mg/mL when, it is suitable with arbutin to the inhibiting rate of tyrosinase activity.
Embodiment 7
1 extract of embodiment, 2 extract of embodiment, the anti-oxidant experiment of 3 extract of embodiment
1 embodiment, 1 extract, 2 extract of embodiment, 3 extract of embodiment remove the experiment of DPPH free radical
1.1 materials and methods
1.1.1 sample
1 extract of embodiment, 2 extract of embodiment, 3 extract of embodiment, vitamin C (positive control).
1.1.2 experimental method
Above-mentioned four kinds of samples are configured to dehydrated alcohol respectively concentration 0.05mg/mL, 0.06mg/mL, 0.07mg/mL,
0.08mg/mL, 0.09mg/mL, 0.1mg/mL, 0.2mg/mL, 0.3mg/mL, 0.4mg/mL, 0.5mg/mL testing sample solution.
2.5mL sample solution is taken, the DPPH standard solution that 2.5mL concentration is 50mg/L is added, mixes immediately, is put into darkroom at room temperature
React 30min, meanwhile, take 2.5mL sample solution, be added 95% ethanol solution 2.5ml be uniformly mixed, 517nm at measurement
Absorbance.DPPH free radical scavenging activity is calculated according to the following formula, the results are shown in Table 3.
The absorbance for the mixed liquor that the sample solution of As:2.5mlLDPPH solution and 2.5mL various concentration forms；
A: for the absorbance of the mixed liquor of test fluid and 2.5mL the dehydrated alcohol composition of 2.5mL various concentration；
A0: for the absorbance of 2.5mL DPPH solution and the mixed solution of 2.5mL dehydrated alcohol composition.
1.2 experimental result
Influence (%) of the extract of the present invention of table 3 to DPPH free radical scavenging activity
2 embodiment, 1 extract, 2 extract of embodiment, 3 extract of embodiment remove the experiment of ABTS free radical
2.1 materials and methods
2.1.1 sample
1 extract of embodiment, 2 extract of embodiment, 3 extract of embodiment, vitamin C (positive control).
2.1.2 experimental method
By above-mentioned four kinds of samples with 95% ethyl alcohol be configured to respectively concentration 0.05mg/mL, 0.06mg/mL, 0.07mg/mL,
0.08mg/mL, 0.09mg/mL, 0.1mg/mL, 0.2mg/mL, 0.3mg/mL, 0.4mg/mL, 0.5mg/mL testing sample solution.
The ABTS solution that precision measures 7mmol/L (is prepared) 0.20ml by distilled water, molten with the potassium peroxydisulfate of the 2.6mmol/L of 0.20ml
Liquid mixing, dilutes 40-50 times, the absorbance value at solution 754nm is made to be maintained at 0.7 ± 0.02, has both obtained the work of ABTS free radical
Liquid is kept in dark place at room temperature.4mlABTS is drawn respectively and 1ml Tea Samples are sufficiently mixed uniformly, 6min is stood, with 95% second
Alcohol measures absorbance value as blank photo at 754nm.Clearance rate is calculated according to the following formula:
Wherein, A0For the absorbance value of ABTS free radical itself, A is sample to the absorbance after ABTS Free Radical
Value.It the results are shown in Table 4.
2.2 experimental result
Influence (%) of the extract of the present invention of table 4 to ABTS free radical scavenging activity
It is above-mentioned anti-oxidant the experimental results showed that, three kinds of embodiment extracts of the invention have good antioxidation,
In, the antioxidation of 1 extract of embodiment is better than 3 extract of embodiment 2 and embodiment.Modern research shows that melanin shape
At related with oxygen radical, therefore by scavenging activated oxygen, the generation of melanin can be effectively reduced, whitening function is played.It is above-mentioned
Anti-oxidant experiment shows that the antioxidant activity of 1 extract of embodiment is better than other embodiments, as a result presses down with l-tyrosine enzymatic activity
System experiment is consistent.
Embodiment 8
1 extract of embodiment, 2 extract of embodiment, 3 extract hygroscopicity of embodiment and moisture retention measurement
1 embodiment, 1 extract, 2 extract of embodiment, the measurement of 3 extract hygroscopicity of embodiment
1.1 materials and methods
1.1.1 sample
1 extract of embodiment, 2 extract of embodiment, 3 extract of embodiment, aloe powder (positive control).
1.1.2 experimental method
Hydroscopicity measurement: at room temperature, each 0.5000g of sample that precision weighs above-mentioned drying to constant weight is respectively placed in training
It supports in ware, culture dish is individually positioned in two driers, is placed with saturated sodium carbonate solution (relative humidity in a drier
43%), to be placed in another drier saturated ammonium sulfate solution (relative humidity 81%), each sample place 4,8,12,24,
36, it 48,60, is weighed after 72h, hydroscopicity is calculated as follows:
It the results are shown in Table 5 and table 6.
1.2 experimental result
The extract hygroscopicity of the present invention of table 5 measures (%) (relative humidity 43%)
The extract hygroscopicity of the present invention of table 6 measures (%) (relative humidity 81%)
Table 5 and table 6 the result shows that, three embodiment extracts and aloe powder are under the conditions of relative humidity 81%, hydroscopicity
It is all larger than the hydroscopicity of relative humidity 43%.Three embodiment extracts have good hydroscopicity, wherein the suction of embodiment 1
Wet rate is substantially better than the extract of other two embodiments.
2 embodiment, 1 extract, 2 extract of embodiment, the measurement of 3 extract moisturizing rate of embodiment
2.1 materials and methods
2.1.1 sample
1 extract of embodiment, 2 extract of embodiment, 3 extract of embodiment, aloe powder (positive control).
2.1.2 experimental method
Moisture retention measurement: at room temperature, each 0.5000g of sample that precision weighs above-mentioned drying to constant weight is respectively placed in training
It supports in ware, the water that mass fraction is 10% is added, slowly shakes, so that sample is fully absorbed moisture, be placed in equipped with dry silica gel
In drier, each sample places 4,8,12,24,36,48,60, weigh after 72h, hydroscopicity is calculated as follows:
(H0Contain water quality, H to place preceding all product1To place sample size after a timing is fixed time).It the results are shown in Table 7.
2.2 experimental result
The extract moisture retention of the present invention of table 7 measures (%)
Table 7 the results show that the moisture retention result of three embodiment extracts of the invention and aloe is consistent with hygroscopicity result,
Three kinds of extracts have good moisture retention, and in for 24 hours, moisturizing rate is all larger than 50%.Wherein, the guarantor of 1 extract of embodiment
Wet effect is substantially better than other two embodiment extracts, and in 72h, moisture retention is still higher than 50%.
Embodiment 9
1 product of the embodiment of the present invention, 2 product of embodiment, 3 product normal individual trial evaluation experimental of embodiment
The preparation of 1 skin whitening, moisturizing frost
The present invention takes above-mentioned compound Chinese medicine extract to be configured to skin whitening, moisturizing frost, is formulated following (by mass percentage):
A phase: stearic acid 3.0%
Vaseline 8.0%
Glycerin monostearate 3.0%
Liquid paraffin 10.0%
Hexadecanol 2.0%
Benzoic acid 0.2%
B phase: purified water 55.0%
Propylene glycol 5.0%
Polyethylene glycol 1500 5.0%
Tween-40 3.0%
Triethanolamine 0.5%
Sodium sulfite 0.1%
C phase: compound Chinese medicine extract 5.0%
D phase: essence 0.2%
Preparation process is as follows:
A phase is separately heated to 80 DEG C with B phase, is thoroughly mixed uniformly, under the conditions of 80 DEG C, B phase is stirred, A is added
Xiang Zhong adds C phase, continues to stir, until D phase is added when temperature is down to 45 DEG C, stirring to room temperature has to get the schizonepeta containing hiding
Skin whitening, moisturizing frost.
Corresponding whitening is made in 1 extract of embodiment, 2 extract of embodiment, 3 extract of embodiment according to the method described above
Moisturiser carries out corresponding human experimentation.
2 materials and methods
2.1 sample
1 extract skin whitening, moisturizing frost of embodiment, 2 extract skin whitening, moisturizing frost of embodiment, 3 extract skin whitening, moisturizing of embodiment
White, commercially available skin whitening, moisturizing is white (main component: witch hazel, Radix Ophiopogonis, kuh-seng, cactus etc.).
2.2 experimental method
Voluntary 80 people of trier (40 people of male, 40 people of women, 18-25 years old age) is chosen, face has pigementation, skin
Dark yellow, the problems such as skin is dry and astringent, no cosmetic allergic contact dermatitis history.Volunteer is divided into 4 groups, every group of 20 people, male to female ratio is fifty-fifty.First
Group tries out 1 product of embodiment；Second group, try out 2 product of embodiment；Third group is tried out 3 product of embodiment, the 4th group, is tried out
Commercially available skin whitening, moisturizing frost.
Application method: after cleaning face sooner or later, taking appropriate Evaluation product to be uniformly applied to face, gently pats and absorbs, and
It gently massages 3-5 minutes, skin is allowed to fully absorb, it is continuous 8 weeks on probation.By questionnaire form, trier is investigated to use production
The subjective assessment of product, including whitening effect, the indexs such as moist, moisturizing and the dry and astringent, excitement of product.It the results are shown in Table 8.
3 experimental results
8 trier of table is to product evaluation application form
Table 8 is trier to the evaluation table of Evaluation product, in table the results show that the embodiment of the present invention 1,2 and of embodiment
3 skin whitening, moisturizing frost product of embodiment has preferable whitening and moist moisture-keeping function, the especially whitening function of 1 product of embodiment
It is substantially better than commercial product, effect is also superior to other two kinds of embodiment products.Three kinds of embodiment products are without allergy and stimulation
Sense, Product Safety are high.
The above embodiment is a preferred embodiment of the present invention, but embodiments of the present invention are not by above-described embodiment
Limitation, other any changes, modifications, substitutions, combinations, simplifications made without departing from the spirit and principles of the present invention,
It should be equivalent substitute mode, be included within the scope of the present invention.
Claims (6)
1. it is a kind of containing hiding schizonepeta have effects that the Chinese traditional medicine composition extract of skin whitening, moisturizing, which is characterized in that the combined extracts by
Following Chinese medicine materials by weight are prepared: hiding schizonepeta 10-30 parts, 10-40 parts of black fruit fructus lycii leaf, Malus toringoides Hughes leaf
10-30 parts, 10-40 parts of highland barley, 10-30 parts of rhizoma polygonati.
2. a kind of schizonepeta containing hiding according to claim 1 has effects that the Chinese traditional medicine composition extract of skin whitening, moisturizing, feature
It is, which is prepared by following Chinese medicine materials by weight: hiding schizonepeta 10-20 parts, black fruit fructus lycii leaf
20-40 parts, 10-20 parts of Malus toringoides Hughes leaf, 20-40 parts of highland barley, 10-20 parts of rhizoma polygonati.
3. a kind of schizonepeta containing hiding according to claim 1 has effects that the Chinese traditional medicine composition extract of skin whitening, moisturizing in preparation beauty
Application in white Moisturizer.
4. application according to claim 3, it is characterised in that: described to have effects that the Chinese medicinal compound extract of skin whitening, moisturizing
Mass percentage in skin whitening, moisturizing skin care item is 0.1-10%.
5. application according to claim 3, it is characterised in that: described to have effects that the Chinese medicinal compound extract of skin whitening, moisturizing
Mass percentage in skin whitening, moisturizing skin care item is 1-8%.
6. application according to claim 3, it is characterised in that: the skin whitening, moisturizing skin care item be whitening and moisturizing facial cleanser and preparation method of facial cleanser,
Skin whitening, moisturizing Cleansing Foam, skin whitening, moisturizing wash one's face frost, skin whitening, moisturizing cream, skin whitening, moisturizing frost, skin whitening, moisturizing lotion, skin whitening, moisturizing water,
Whitening moisturizing face masque, skin whitening, moisturizing gel or skin whitening, moisturizing soap.
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| CN107007526A (en) * | 2017-05-09 | 2017-08-04 | 上海绰然生物科技有限公司 | A kind of Preparation method and use of highland barley bran extract |
| CN108524392A (en) * | 2018-05-28 | 2018-09-14 | 上海应用技术大学 | A kind of sunscreen composition and suncream containing barley young leaf powder and calophyllum inophyllum kernel oil |
| CN111374915A (en) * | 2018-12-29 | 2020-07-07 | 上海洵都医疗科技有限公司 | Anti-aging composition and preparation method thereof |
| CN110227050B (en) * | 2019-07-26 | 2022-08-09 | 嘉兴言肌草生物技术有限公司 | Anti-aging repairing slow-release essence and anti-aging repairing mask prepared from same |
| CN113197817A (en) * | 2021-04-16 | 2021-08-03 | 云南白药集团股份有限公司 | Whitening application of pineapple extract composition |
| CN113230166A (en) * | 2021-04-16 | 2021-08-10 | 云南白药集团股份有限公司 | Mask liquid, preparation method and application thereof |
| CN114010560B (en) * | 2021-11-18 | 2024-02-23 | 班戈卓攀健康产业有限责任公司 | Compound Tibetan herba schizonepetae composition with skin color brightening, anti-allergy and soothing effects and preparation method thereof |
-
2016
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Non-Patent Citations (4)
| Title |
|---|
| 藏叶"俄色叶"的质量标准研究;夏冬梅;《中国优秀硕士学位论文全文数据库医药卫生科技辑》;20150615(第6期);E056-438页 |
| 藏荆芥乙酸乙酯部位化学成分及抗炎、镇痛活性研究;胡丹丹;《中国优秀硕士论文全文数据库医药卫生科技辑》;20160815(第8期);E057-150页 |
| 青稞β-葡聚糖理化性质、流变性质以及在化妆品中的应用;董磊;《中国优秀硕士学位论文全文数据库工程科技I辑》;20160215(第2期);B016-91页 |
| 黑果枸杞叶总黄酮的体外抗氧化活性研究;李进等;《食品科学》;20100701;第21卷(第13期);第259-262页 |
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