CN107007526A - A kind of Preparation method and use of highland barley bran extract - Google Patents
A kind of Preparation method and use of highland barley bran extract Download PDFInfo
- Publication number
- CN107007526A CN107007526A CN201710321368.1A CN201710321368A CN107007526A CN 107007526 A CN107007526 A CN 107007526A CN 201710321368 A CN201710321368 A CN 201710321368A CN 107007526 A CN107007526 A CN 107007526A
- Authority
- CN
- China
- Prior art keywords
- highland barley
- barley bran
- bran extract
- preparation
- solvent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/10—General cosmetic use
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Microbiology (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Mycology (AREA)
- Botany (AREA)
- Biotechnology (AREA)
- Engineering & Computer Science (AREA)
- Dermatology (AREA)
- Medicines Containing Plant Substances (AREA)
- Cosmetics (AREA)
Abstract
The invention provides a kind of preparation method of highland barley bran extract, including it is 1 that highland barley bran is pressed into solid-liquid ratio:8~1:20 addition solvent, is extracted under 30~70 DEG C of stirring in water bath, and the time is 2~6h, extraction times 1~3 time；Filtering, removes filter residue, obtains yellow or brown color supernatant liquid；Concentrated by rotary evaporation, removes solvent, obtains paste thick liquid；Acid solution washing is added, alkali lye regulation pH is 9 ~ 10；Concentration, organic solvent extraction；Concentrated extract, dries, obtains highland barley bran extract.Present invention also offers a kind of application of the highland barley bran extract prepared using the above method in personal nursing.The above-mentioned highland barley bran extract prepared is subjected to ultraviolet test, acquired results have ultraviolet absorption peak in 280nm or so.The composition is fresh rare in the result of study of highland barley.Discovery and preparation of the present invention to alkaloid in highland barley bran are studied, and the utilization and research and development for highland barley provide a thinking.
Description
Technical field
The invention belongs to field of biology, it is related to a kind of highland barley bran, specifically a kind of highland barley bran extract
Preparation method and use.
Background technology
Highland barley（Hordeum Vulgare L. var. nudum Hook. f）It is that Qinghai-xizang Plateau Region has distinctive paddy
Crops are planted, are the high and cold farming region staple food crops that Qinghai-Tibet Platean is harvested for one time each year, also known as barley rice, naked barley, meter Mai, are belonged to
One of barley mutation.It is mainly distributed on 4 200~4 500m Qinghai-Tibet extremely frigid zones, including height above sea level Tibet, Qinghai, Gansu Province are sweet
The Tibetan Autonomous Prefecture of Deqen of southern autonomous region of Tibetan, Szechwan Ganzi state and Abbe state and Yunnan.Highland barley by color phenol be white highland barley,
Cyanine highland barley, black highland barley and purple highland barley etc..Highland barley is the food of high protein, high microsteping, homovitamin, low fat and low sugar, is paddy
Good merchantable brand in class crop.Research report, highland barley is rich in the physiologically active ingredients such as beta glucan, tocol, flavones, amino acid, tool
There are anti arteriosclerosis, regulation blood glucose, reduction cholesterol and improve immunity, Liquidity limit and organic matter to influence material generation
The function such as thank, enjoy the favor of consumers in general.
Alkaloid（alkaloids）It is often referred to be present in the class organic compounds containing nitrogen in organism, isolating protein, ammonia
Base acid, polypeptide, nucleic acid, nucleotides, vitamins, choline, porphyrin and low molecular open chain fatty acid amine.From structure, greatly
It is general that there is alkalescence and physiologically active all containing complicated nitrogen heterocyclic ring in the molecule of most alkaloids.It is distributed in plant kingdom
It is relatively wide, it is a larger class in plant time metabolite, the alkaloid separated at present from plant there are about 5000~7000
Kind.According to different chemical structural types, alkaloid can be divided into pyroles, quinolines, iloquinoline derivative, piperidines, indoles,
Henbane alkanes, terpene, steroid, peptides etc., wherein isoquinoline alkaloid are distributed more widely and dose known amounts are most.Because of it
The diversity of structure and different pharmacological activity, such as antibacterial, antitumor, easing pain and diminishing inflammation, antiviral, hypotensive, regulation are immune
Function, makes it have huge application potential in terms of medicine, health, in recent years the study hotspot as people.
In recent years, this green natural cereal with healthcare function of highland barley, was gradually paid attention to by different industries.To it
Active component research report essentially consist in beta glucan, flavonoids, amino acid.But the research for alkaloid in highland barley and
Report fresh rare, present invention aims at the discovery of alkaloid in highland barley bran and preparation, utilization and research for highland barley are provided
One thinking.
The content of the invention
For above-mentioned technical problem of the prior art, the invention provides a kind of preparation method of highland barley bran extract
And purposes, it is biological that the Preparation method and use of described this highland barley bran extract will solve highland barley bran of the prior art
Alkali is not easy the technical problem extracted.
The invention provides a kind of preparation method of highland barley bran extract, comprise the following steps：
1) it is 1 by solid-liquid ratio by highland barley bran:8~1:20 addition solvent, is extracted, the time is under 30~70 DEG C of stirring in water bath
2~6h, extraction times 1~3 time；
2) filter, remove filter residue, obtain yellow or brown color supernatant liquid；
3) concentrated by rotary evaporation, removes solvent, obtains paste thick liquid；
4) acid solution washing is added, then it is 9 ~ 10 to adjust pH with alkali lye；
5) concentrate, organic solvent extraction；
6) concentrated extract, dries, obtains highland barley bran extract.
Further, step 1）Described in solvent be ethanol or pH=3~4 that mass percent concentration is 60 ~ 80%
Acetum.
Further, step 1）Described in solvent be ethanol, glacial acetic acid and water mixture, described ethanol, ice vinegar
The volume ratio of acid and water is 70 ~ 90：0.4~0.6:15~20；The mass percent concentration of described ethanol is 60 ~ 80%.
Further, step 4）Acid in acid solution is organic acid, and described organic acid is acetic acid or citric acid, its quality
Percent concentration is 0.5 ~ 2%, and described alkali lye is sodium hydroxide solution, and its mass percent concentration is 5 ~ 20%.
Further, step 5）Described in organic solvent be petroleum ether, chloroform, ethanol, isopropanol or n-butanol.
Present invention also offers a kind of application of the highland barley bran extract prepared using the above method in personal nursing.
Further, in the applications described above, it is water, glycerine, butanediol, olive oil, castor to prepare the solvent used in liquid
Sesame oil or above-mentioned mixture.
Present invention also offers a kind of personal-care supplies containing highland barley bran extract, by glycerine, castor oil, highland barley
Wheat bran extract, Phenoxyethanol, parahydroxyacet-ophenone and water composition, glycerine, castor oil, highland barley bran extract, Phenoxyethanol,
The mass ratio of parahydroxyacet-ophenone and water is 5~25:0~5:1:0.5~1:0.1~1:67~93.4.
The present invention is compared with prior art, and its technological progress is significant.The above-mentioned highland barley bran prepared is extracted
Thing carries out ultraviolet test, and acquired results have ultraviolet absorption peak in 280nm or so.The composition is fresh few in the result of study of highland barley
See.Discovery and preparation of the present invention to alkaloid in highland barley bran are studied, and the utilization and research and development for highland barley provide one
Individual thinking.
Brief description of the drawings
Fig. 1 is the UV scanning collection of illustrative plates of highland barley bran extract prepared by the present invention of the embodiment of the present invention 4.
Embodiment
By combining case study on implementation in detail below, the present invention is further elaborated, but is not construed as to the present invention's
Limitation.In the case where not departing from spirit of the invention, content and scope, to repairing on the report and preparation method of present invention
Change, belong to the scope of the invention.
Embodiment 1：The preparation method one of highland barley bran extract
A certain amount of highland barley bran is weighed, is 1 by solid-liquid ratio:10 add solvent（Mass percent concentration is 70% ethanol：Ice
Acetic acid：Water=80:0.5:19.5）, 60 DEG C of stirring in water bath extract 2h, filter to obtain yellow or yellowish-brown liquid, and spin concentration removes molten
Agent, obtains medicinal extract, uses mass percent concentration to be washed for 1% acetic acid solution, and mass percent concentration is adjusted for 10%NaOH liquid
PH is 9.50, and isopropanol is extracted, and takes organic phase to concentrate, and dries, obtains highland barley bran extract.
Embodiment 2：The preparation method of composition two of the thing containing active Chinese drug component
A certain amount of highland barley bran is weighed, is 1 by solid-liquid ratio:12 add mass percent concentration for 70% ethanol, 60 DEG C of water-baths
Stirring extraction 1.5h, filtering；Repeat to extract once, merge extract solution, obtain yellow or yellowish-brown liquid, spin concentration removes molten
Agent, obtains medicinal extract, uses mass percent concentration to be washed for 1% acetic acid solution, and mass percent concentration is adjusted for 10%NaOH liquid
PH is 9.50, and isopropanol is extracted, and takes organic phase to concentrate, and dries, obtains highland barley bran extract.
Embodiment 3：The identification of highland barley bran extract
The extract of a certain amount of above-mentioned preparation is weighed, uses mass percent concentration to be dissolved for 80% ethanol, filters to take
Clear liquid.Measure 10mL and prepare liquid, add 2~3 and drip KI-I2Liquid or the silico-tungstic acid liquid of mass fraction 6%.Its result is as follows：
| Developer | Phenomenon |
| KI-I2 | Yellowish-brown is precipitated |
| 6% silico-tungstic acid liquid | White precipitate |
The extract prepared through above-mentioned qualification result, the method illustrated by the present invention by raw material of highland barley bran, is determined as that this is carried
Take and contain composition of alkaloids in thing.
Embodiment 4：Highland barley bran extract ultraviolet spectra is tested
By highland barley extractive be configured to mass percent concentration be 2% solution and carry out UV scanning, wave-length coverage 200~
800nm。
The instrument of use：UV-2100 type ultraviolet specrophotometers, Prism Optical Technology Co.
Scanning result shows that the highland barley bran extract that the method illustrated by the present invention is obtained has absorption in 280nm or so
Peak, alkaloid about has absorbance at 280~292nm.With reference to above-mentioned qualification result, the highland barley bran obtained by the present invention is judged
Contain composition of alkaloids in extract.
Embodiment 5：Contain highland barley bran extract liquid
Prepare the raw material needed and method is as follows：
Step is as follows：
1）A group raw materials are added, 85 DEG C, stirring to dissolving are heated to；
2）Cool down, be cooled to 5565℃；
3）B group raw materials are added, is evenly stirred until and is completely dissolved, are discharged.
Claims (8)
1. a kind of preparation method of highland barley bran extract, it is characterised in that comprise the following steps：
It is 1 that highland barley bran is pressed into solid-liquid ratio:8~1:20 addition solvent, is extracted, the time is 2 under 30~70 DEG C of stirring in water bath
~6h, extraction times 1~3 time；
Filtering, removes filter residue, obtains yellow or brown color supernatant liquid；
Concentrated by rotary evaporation, removes solvent, obtains paste thick liquid；
Acid solution washing is added, it is 9 ~ 10 then to adjust pH with alkali lye again；
Concentration, organic solvent extraction；
Concentrated extract, dries, obtains highland barley bran extract.
2. a kind of preparation method of highland barley bran extract according to claim 1, it is characterised in that：Step 1）Described in
Solvent be ethanol that mass percent concentration is 60 ~ 80% or the acetum of pH=3~4.
3. a kind of preparation method of highland barley bran extract according to claim 1, it is characterised in that：Step 1）Described in
Solvent be ethanol, glacial acetic acid and water mixture, the volume ratio of described ethanol, glacial acetic acid and water is 70 ~ 90：0.4~0.6:
15~20；The mass percent concentration of described ethanol is 60 ~ 80%.
4. a kind of preparation method of highland barley bran extract according to claim 1, it is characterised in that：Step 4）In acid solution
Acid be organic acid, described organic acid is acetic acid or citric acid, and its mass percent concentration is 0.5 ~ 2%, described alkali lye
For sodium hydroxide solution, its mass percent concentration is 5 ~ 20%.
5. a kind of preparation method of highland barley bran extract according to claim 1, it is characterised in that：Step 5）Described in
Organic solvent be petroleum ether, chloroform, ethanol, isopropanol or n-butanol.
6. a kind of application of highland barley bran extract prepared by claim 1 methods described in personal nursing.
7. application according to claim 6, it is characterised in that：Prepare liquid used in solvent for water, glycerine, butanediol,
Olive oil, castor oil or above-mentioned mixture.
8. a kind of personal-care supplies containing highland barley bran extract, it is characterised in that：By glycerine, castor oil, highland barley bran
Extract, Phenoxyethanol, parahydroxyacet-ophenone and water composition, glycerine, castor oil, highland barley bran extract, Phenoxyethanol, to hydroxyl
The mass ratio of benzoylformaldoxime and water is 5~25:0~5:1:0.5~1:0.1~1:67~93.4.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710321368.1A CN107007526A (en) | 2017-05-09 | 2017-05-09 | A kind of Preparation method and use of highland barley bran extract |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710321368.1A CN107007526A (en) | 2017-05-09 | 2017-05-09 | A kind of Preparation method and use of highland barley bran extract |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107007526A true CN107007526A (en) | 2017-08-04 |
Family
ID=59450442
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710321368.1A Withdrawn CN107007526A (en) | 2017-05-09 | 2017-05-09 | A kind of Preparation method and use of highland barley bran extract |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107007526A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109007818A (en) * | 2018-06-21 | 2018-12-18 | 江苏省农业科学院 | The preparation method and its purposes in terms of cellular anti-oxidant of a kind of wheat extract |
| CN114304688A (en) * | 2021-11-25 | 2022-04-12 | 中粮集团有限公司 | Zein coating agent, probiotic preparation coated by using zein coating agent and preparation method of probiotic preparation |
| CN114480548A (en) * | 2022-03-22 | 2022-05-13 | 劲牌持正堂药业有限公司 | Preparation method and application of highland barley bran polypeptide |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101016297A (en) * | 2007-03-08 | 2007-08-15 | 上海交通大学 | Method of extracting reserpine from davilpepper |
| CN101125119A (en) * | 2007-07-18 | 2008-02-20 | 西藏宇妥藏药研究有限公司 | Tibetan materia medica with whitening anti-senility function and application thereof |
| CN101139320A (en) * | 2007-09-01 | 2008-03-12 | 福州大学 | Method for separating nuciferine and lotus leaf flavone from lotus leaf |
| CN101696381A (en) * | 2009-10-23 | 2010-04-21 | 中国科学院西北高原生物研究所 | Novel process for preparing highland barley flavone extract and application thereof in health wine |
| CN101455794B (en) * | 2008-12-31 | 2011-08-17 | 西藏自治区高原生物研究所 | Use of highland barley bran |
| CN103275012A (en) * | 2013-06-08 | 2013-09-04 | 中国科学院过程工程研究所 | Preparation method and application of Maca alkaloid |
| CN106420531A (en) * | 2016-11-24 | 2017-02-22 | 青岛科技大学 | Traditional Chinese medicine composite extract containing nepeta angustifolia and having skin whitening and moisturizing function and application |
-
2017
- 2017-05-09 CN CN201710321368.1A patent/CN107007526A/en not_active Withdrawn
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101016297A (en) * | 2007-03-08 | 2007-08-15 | 上海交通大学 | Method of extracting reserpine from davilpepper |
| CN101125119A (en) * | 2007-07-18 | 2008-02-20 | 西藏宇妥藏药研究有限公司 | Tibetan materia medica with whitening anti-senility function and application thereof |
| CN101139320A (en) * | 2007-09-01 | 2008-03-12 | 福州大学 | Method for separating nuciferine and lotus leaf flavone from lotus leaf |
| CN101455794B (en) * | 2008-12-31 | 2011-08-17 | 西藏自治区高原生物研究所 | Use of highland barley bran |
| CN101696381A (en) * | 2009-10-23 | 2010-04-21 | 中国科学院西北高原生物研究所 | Novel process for preparing highland barley flavone extract and application thereof in health wine |
| CN103275012A (en) * | 2013-06-08 | 2013-09-04 | 中国科学院过程工程研究所 | Preparation method and application of Maca alkaloid |
| CN106420531A (en) * | 2016-11-24 | 2017-02-22 | 青岛科技大学 | Traditional Chinese medicine composite extract containing nepeta angustifolia and having skin whitening and moisturizing function and application |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109007818A (en) * | 2018-06-21 | 2018-12-18 | 江苏省农业科学院 | The preparation method and its purposes in terms of cellular anti-oxidant of a kind of wheat extract |
| CN114304688A (en) * | 2021-11-25 | 2022-04-12 | 中粮集团有限公司 | Zein coating agent, probiotic preparation coated by using zein coating agent and preparation method of probiotic preparation |
| CN114480548A (en) * | 2022-03-22 | 2022-05-13 | 劲牌持正堂药业有限公司 | Preparation method and application of highland barley bran polypeptide |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103037879A (en) | Method for preparing novel processed ginseng or an extract thereof, the usually minute ginsenoside content of which is increased | |
| CN107007526A (en) | A kind of Preparation method and use of highland barley bran extract | |
| CN104784210A (en) | Preparation method and application of skipjack extract with uric acid reduction effect | |
| CN114874344B (en) | Preparation method and application of alkali-extracted polysaccharides of highland barley tender leaves | |
| CN104800252B (en) | A kind of refined polyphenol and its preparation method and application with tumor inhibition effect | |
| CN106109515A (en) | A kind of extracting method of total Flavonoids from Suaeda salsa | |
| CN105663444A (en) | Compound immunity-enhancing and aging-resisting agent and preparation method thereof | |
| CN101229335A (en) | Method of enzyme method preparing smilax scobinicaulis total saponin extract | |
| CN105106816B (en) | Protect the traditional Chinese medicine health care product preparation and preparation method thereof of chemical damage | |
| CN103113489B (en) | Method of purifying polysaccharide of Xinjiang jun dates | |
| CN101313939A (en) | Corydalis saxicola total alkaloid, preparation method and uses thereof | |
| CN107089992A (en) | A kind of curcuma total sesquiterpene and three kinds of sequiterpene monomers and preparation method and purposes | |
| CN106047569A (en) | Preparation process for medicinal liquor | |
| CN105412613B (en) | A kind of formula and its processing method for senile dementia | |
| WO2022142561A1 (en) | Quaternary ammonium salt alkaloid compound in noni enzyme, preparation method therefor and application thereof | |
| CN108392508A (en) | Application of the white lotus parthenium extract in preparing anti-inflammatory drug | |
| CN111850080B (en) | Preparation method of pithecellobium clypearia polypeptide and preparation method of pithecellobium clypearia polypeptide liposome | |
| CN103638031B (en) | The preparation method of compound quinatic acid and the application in preparing glycosidase inhibitor | |
| CN103087119B (en) | One grows flax Cerebrosides compound and extraction and separation method thereof | |
| CN102258548B (en) | Method for preparing nardostachyos root and rhizome volatile oil beta-cyclodextrin inclusion compound | |
| CN104911068A (en) | Taxus media health-care wine and making method thereof | |
| CN101390979B (en) | Raisin tree seed extract and preparation method and use thereof in preparing anti-tumor medicine | |
| CN104946460B (en) | A kind of Taxus x media milk taste Wine and preparation method thereof | |
| CN106421455A (en) | Sugar sorghum extract as well as preparation method and application thereof | |
| CN115192624B (en) | Extraction process and application of rhododendron simsii |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20170804 |
|
| WW01 | Invention patent application withdrawn after publication |