CN106109515A - A kind of extracting method of total Flavonoids from Suaeda salsa - Google Patents
A kind of extracting method of total Flavonoids from Suaeda salsa Download PDFInfo
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- CN106109515A CN106109515A CN201610624968.0A CN201610624968A CN106109515A CN 106109515 A CN106109515 A CN 106109515A CN 201610624968 A CN201610624968 A CN 201610624968A CN 106109515 A CN106109515 A CN 106109515A
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- total flavonoids
- suaeda salsa
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- 241000586290 Suaeda salsa Species 0.000 title claims abstract description 21
- 229930003935 flavonoid Natural products 0.000 title claims abstract description 19
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- 235000017173 flavonoids Nutrition 0.000 title claims abstract description 19
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- 229930003944 flavone Natural products 0.000 claims abstract description 33
- 235000011949 flavones Nutrition 0.000 claims abstract description 33
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- 239000000284 extract Substances 0.000 claims abstract description 22
- 238000000605 extraction Methods 0.000 claims abstract description 21
- 239000003480 eluent Substances 0.000 claims abstract description 17
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- 108090000790 Enzymes Proteins 0.000 claims abstract description 13
- 102000004190 Enzymes Human genes 0.000 claims abstract description 13
- 150000001875 compounds Chemical class 0.000 claims abstract description 13
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- 238000001914 filtration Methods 0.000 claims abstract description 12
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- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 claims description 26
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- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 abstract description 4
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- JMGZEFIQIZZSBH-UHFFFAOYSA-N Bioquercetin Natural products CC1OC(OCC(O)C2OC(OC3=C(Oc4cc(O)cc(O)c4C3=O)c5ccc(O)c(O)c5)C(O)C2O)C(O)C(O)C1O JMGZEFIQIZZSBH-UHFFFAOYSA-N 0.000 description 1
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- 241000274938 Suaeda heteroptera Species 0.000 description 1
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- JLDSOYXADOWAKB-UHFFFAOYSA-N aluminium nitrate Inorganic materials [Al+3].[O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O JLDSOYXADOWAKB-UHFFFAOYSA-N 0.000 description 1
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- 239000008280 blood Substances 0.000 description 1
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- 150000001746 carotenes Chemical class 0.000 description 1
- 235000005473 carotenes Nutrition 0.000 description 1
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- 229910052802 copper Inorganic materials 0.000 description 1
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- IVTMALDHFAHOGL-UHFFFAOYSA-N eriodictyol 7-O-rutinoside Natural products OC1C(O)C(O)C(C)OC1OCC1C(O)C(O)C(O)C(OC=2C=C3C(C(C(O)=C(O3)C=3C=C(O)C(O)=CC=3)=O)=C(O)C=2)O1 IVTMALDHFAHOGL-UHFFFAOYSA-N 0.000 description 1
- 239000000686 essence Substances 0.000 description 1
- ZINJLDJMHCUBIP-UHFFFAOYSA-N ethametsulfuron-methyl Chemical compound CCOC1=NC(NC)=NC(NC(=O)NS(=O)(=O)C=2C(=CC=CC=2)C(=O)OC)=N1 ZINJLDJMHCUBIP-UHFFFAOYSA-N 0.000 description 1
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- HVQAJTFOCKOKIN-UHFFFAOYSA-N flavonol Natural products O1C2=CC=CC=C2C(=O)C(O)=C1C1=CC=CC=C1 HVQAJTFOCKOKIN-UHFFFAOYSA-N 0.000 description 1
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- FDRQPMVGJOQVTL-UHFFFAOYSA-N quercetin rutinoside Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 FDRQPMVGJOQVTL-UHFFFAOYSA-N 0.000 description 1
- 230000004223 radioprotective effect Effects 0.000 description 1
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- IKGXIBQEEMLURG-BKUODXTLSA-N rutin Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](C)O[C@@H]1OC[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 IKGXIBQEEMLURG-BKUODXTLSA-N 0.000 description 1
- ALABRVAAKCSLSC-UHFFFAOYSA-N rutin Natural products CC1OC(OCC2OC(O)C(O)C(O)C2O)C(O)C(O)C1OC3=C(Oc4cc(O)cc(O)c4C3=O)c5ccc(O)c(O)c5 ALABRVAAKCSLSC-UHFFFAOYSA-N 0.000 description 1
- 235000005493 rutin Nutrition 0.000 description 1
- 229960004555 rutoside Drugs 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- LPXPTNMVRIOKMN-UHFFFAOYSA-M sodium nitrite Substances [Na+].[O-]N=O LPXPTNMVRIOKMN-UHFFFAOYSA-M 0.000 description 1
- 229940082787 spirulina Drugs 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
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- NCYCYZXNIZJOKI-UHFFFAOYSA-N vitamin A aldehyde Natural products O=CC=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C NCYCYZXNIZJOKI-UHFFFAOYSA-N 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
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- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
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- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
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- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
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Abstract
The present invention relates to the extraction process of flavone, particularly relate to the extracting method of a kind of total Flavonoids from Suaeda salsa, comprise the following steps: S1. plucks Herba suadeae glaucae stem and leaf, cleans, is dried, pulverizes, sieves, obtains Herba suadeae glaucae powder；S2. weigh Herba suadeae glaucae powder and add distilled water, adjusting pH value, adding enzymolysis under the conditions of compound enzyme vacuum tightness, extraction, be centrifuged, be filtrated to get supernatant a and filtering residue；S3. take supernatant a to concentrate, add ethanol, extraction, be centrifuged, filter to obtain supernatant b；S4. being concentrated by supernatant b, then add extract extraction, separate to obtain supernatant c, concentration, lyophilization obtain total flavones crude product；S5. by total flavones crude product, dissolving with distilled water, cross macroporous resin column, ethanol elution, collect eluent, concentration, lyophilization obtain refined total flavonoids.Extracting method technique in the present invention is simple, and easy to operate, the time is short, improves the extraction ratio of chromocor compound, and effectively removes chlorophyll, polysaccharide and other miscellaneous point, improves chromocor compound product purity.
Description
Technical field
The present invention relates to the extraction process of flavone, particularly relate to the extracting method of a kind of total Flavonoids from Suaeda salsa.
Background technology
Suaeda salsa (Suacda salsa), has another name called Suaeda heteroptera, Suaeda salsa, wild on the salt flat in tidal land or saltern,
Pollution-free, it is referred to as standard green food.The nutritional labeling of Suaeda salsa is the abundantest, and the protein content of its fresh and tender stem and leaf accounts for
The 40% of dry, similar with Semen sojae atricolor；And the fat content of seed is up to 36.4%, far above Semen sojae atricolor (18.8%).It addition, alkali
Fluffy stem and leaf contains aminoacid, vitamin, carotene and Ca, P, Fe, Cu, Zn, Mn, Se etc. necessary to substantial amounts of human body micro-
Secondary element, many of which index is all higher than spirulina.It is edible as can be seen here, health care, medical value are big, non-normal food
Reached.
Total flavones is the nutrient of needed by human, and inside of human body can not synthesize and can only absorb from diet, is that a class is important
Excited state active substance, be the indispensable body physiological function regulator of life movement.Total flavones with flavone, flavonol,
The forms such as flavanone exist, and have biological effect and pharmacological action widely；Research shows that total flavones has following effect: anti-
Pathogenic microorganism, infection, anti-inflammatory, antiulcer, antiallergic, antioxidation, defying age, resisting fatigue, arteriosclerosis, antithrombotic
Formation, radioprotective, mutation, antitumor, the liver protecting and cardiac muscle, purification blood, improve microcirculating state, promote tissue again
Raw, regulate body physiological function comprehensively.Main extraction Huang from Folium Ginkgo, Semen sojae atricolor, Folium Crataegi, Folium Nelumbinis, Pericarppium arachidis hypogaeae etc.
Ketonic compound, and the research with Folium Ginkgo and Semen sojae atricolor is the most deep, the most also has been reported that extraction chromocor compound from Herba suadeae glaucae, carries
Taking technique many employings organic solvent ethanol extracts, but it is low to there is problems of chromocor compound extraction ratio, during extraction
Between long, and containing chlorophyll, polysaccharide and other miscellaneous point in chromocor compound, affect product purity.
Summary of the invention
The technical problem to be solved is: provide the extracting method of a kind of total Flavonoids from Suaeda salsa, the method work
Skill is simple, and easy to operate, the time is short, improves the extraction ratio of chromocor compound, and effectively removes chlorophyll, polysaccharide and other are miscellaneous
Point, improve chromocor compound product purity.
For solving above-mentioned technical problem, the technical scheme is that
The extracting method of a kind of total Flavonoids from Suaeda salsa, described extracting method comprises the following steps:
S1. pluck fresh Herba suadeae glaucae stem and leaf, through over cleaning, be dried and after pulverizing, cross 20-80 mesh sieve, obtaining Herba suadeae glaucae powder；
S2. weigh the Herba suadeae glaucae powder in S1 and add the distilled water of described Herba suadeae glaucae grain weight amount 10-18 times, adjusting pH value at 4.8-6
Between, be subsequently adding compound enzyme and under the conditions of the vacuum tightness of 200-500mpa enzymolysis 15-25min, extract 1-2.5h, from
The heart, it is filtrated to get supernatant a and filtering residue；
S3. the supernatant a taking S2 gained concentrates, and the volumetric concentration then adding concentrated solution volume 10-20 times is 45%-
The ethanol of 80%, extracts 1-3h, is centrifuged, is filtrated to get supernatant b；
S4. the supernatant b obtained by S3 concentrates, and then adds the extract of concentrated solution volume 1-2 times, stirs, stands,
Being extracted liquid layer and supernatant layer, through isolated supernatant c, concentrated supernatant c, that lyophilization obtains total flavones is thick
Product；
S5. the total flavones crude product obtained by S4, dissolves with distilled water, then crosses macroporous resin column, and by volumetric concentration is
The ethanol of 50%-75% carries out eluting to it, collects eluent, then obtains refined total yellow by eluent concentration, lyophilization
Ketone.
As the preferred technical scheme of one, the addition of described compound enzyme is the 0.03%-of described Herba suadeae glaucae grain weight amount
0.05%.
As the technical scheme of a kind of improvement, described compound enzyme is cellulase, pectase and papain, and described
The ratio of cellulase, pectase and papain is 1.1-1.7:0.7-1:0.3.
As the preferred technical scheme of one, the hydrolysis temperature of described enzymolysis is 40-65 DEG C.
As the technical scheme of a kind of improvement, take the filtering residue that S2 obtains, then extract 1-2h with the EtOH Sonicate of 75%, from
The heart, filtration, the filtrate obtained merges with described supernatant a.
As the preferred technical scheme of one, the concentration of the ethanol in described S3 is 65%.
As the preferred technical scheme of one, in described S5, macroporous resin column selects HPD-722 or D101-B.
As the preferred technical scheme of one, the extract in described S4 is petroleum ether, chloroform, ethyl acetate, n-butyl alcohol
In one；Or the mixed liquor of ethyl acetate and n-butyl alcohol.
As a kind of further preferred technical scheme, the extract in described S4 is the mixing of ethyl acetate and n-butyl alcohol
Liquid, and described ethyl acetate mixes according to the ratio of 1:1 with n-butyl alcohol.
After have employed technique scheme, the invention has the beneficial effects as follows:
Compared with prior art, the present invention mainly has the advantage that
(1) use compound enzyme, under high pressure vacuum airtight condition, Herba suadeae glaucae is carried out enzymolysis, make the total flavones in cell wall complete
Entirely discharge, and shorten enzymolysis time and extraction time, improve the extraction ratio of total flavones simultaneously, total flavones extraction rate reached can be made
To 267mg/g；
(2) by alcohol steep, extract extraction can effectively remove polysaccharide molecule and chlorophyll；
(3) being enriched with chromocor compound by macroporous resin column, total flavones is carried out point by elution further
From, purification, improve the purity of chromocor compound, and after purification in extract in Suaeda salsa flavones content reach 84.6%；
(4) present invention process is simple and convenient to operate, organic solvent toxicity is little, easy recovery, feasibility are good.
Detailed description of the invention
In order to make the purpose of the present invention, technical scheme and advantage clearer, below in conjunction with embodiment, to the present invention
It is further elaborated.Should be appreciated that specific embodiment described herein, only in order to explain the present invention, is not used to
Limit the present invention.
The present invention uses NaNO2-Al(NO3)3Method measures the content of total flavones in Suaeda salsa.
The drafting of standard curve: first-selected accurately weighing dries rutin standard substance (purity 98%) 20mg to constant weight, with
60% ethanol is settled to 100mL as mother solution.Then draw mother solution 0mL, 2.0mL, 4.0mL, 6.0mL, 8.0mL, 10mL to split
In 25mL volumetric flask, respectively add 5%NaNO21mL, shakes up, and stands 6min, is subsequently adding 10%Al (NO3)3Solution 1.0mL,
Shake up, stand 6min, add NaOH solution 10mL of 1mol/L, finally with 60% ethanol dilution to scale, standing 15min in
Measure absorbance under 510nm, Absorbance versus concentration set up regression equation: Y=0.0895+0.1024 (r=0.9993).
Example 1
The extracting method of a kind of total Flavonoids from Suaeda salsa, comprises the following steps:
S1. pluck fresh Herba suadeae glaucae stem and leaf, through over cleaning, be dried and after pulverizing, cross 60 mesh sieves, obtaining Herba suadeae glaucae powder；
S2. weighing the Herba suadeae glaucae powder 5g in S1 and add the distilled water of described Herba suadeae glaucae grain weight amount 10 times, adjusting pH value is 4.8, so
The compound enzyme (ratio of cellulase, pectase and papain is 1:0.7:0.3) of rear addition 0.03% at 500mpa
Vacuum tightness under the conditions of enzymolysis 15min, and hydrolysis temperature controls at 45 DEG C, extracts 1.5h, is centrifuged, is filtrated to get supernatant a
And filtering residue；
S3. the supernatant a taking S2 gained concentrates, and then adds the second that volumetric concentration is 60% of concentrated solution volume 10 times
Alcohol, extracts 1.5h, is centrifuged, is filtrated to get supernatant b；
S4. the supernatant b obtained by S3 concentrates, and then adds the isopyknic petroleum ether of concentrated solution, stirs, stands,
To extraction liquid layer and supernatant layer, obtain total flavones crude product through isolated supernatant c, concentrated supernatant c, lyophilization；
S5. the total flavones crude product obtained by S4, dissolves with distilled water, then crosses macroporous resin column (HPD-722), uses volume
Concentration is that 60% ethanol carries out eluting, eluent flow rate 1.5ml/min to it, collects eluent, then carries out dense to eluent
Contracting, lyophilization obtain refined total flavonoids.The total flavones 223mg/g extracted in Herba suadeae glaucae under these process conditions, purity is
80.1%.
Example 2
S1. pluck fresh Herba suadeae glaucae stem and leaf, through over cleaning, be dried and after pulverizing, cross 80 mesh sieves, obtaining Herba suadeae glaucae powder；
S2. weighing the Herba suadeae glaucae powder 5g in S1 and add the distilled water of described Herba suadeae glaucae grain weight amount 15 times, adjusting pH value is 5, then
Add the compound enzyme (ratio of cellulase, pectase and papain is 1.5:1:0.3) of 0.04% and 500mpa's
Enzymolysis 20min under the conditions of vacuum tightness, and hydrolysis temperature controls at 50 DEG C, extracts 2h, is centrifuged, is filtrated to get supernatant a and filter
Slag；
S3. the supernatant a taking S2 gained concentrates, and then adds the second that volumetric concentration is 75% of concentrated solution volume 15 times
Alcohol, extracts 2.5h, is centrifuged, is filtrated to get supernatant b；
S4. the supernatant b that obtained by S3 concentrates, then add concentrated solution volume 2 times extract (ethyl acetate with just
Butanol presses 1:1 mixing), stir, stand, be extracted liquid layer and supernatant layer, through isolated supernatant c, concentrate supernatant
Liquid c, lyophilization obtain total flavones crude product；
S5. the total flavones crude product obtained by S4, dissolves with distilled water, then crosses macroporous resin column (HPD-722), uses volume
Concentration is that 65% ethanol carries out eluting, eluent flow rate 2ml/min to it, collects eluent, then eluent is concentrated,
Lyophilization obtains Herba suadeae glaucae total flavones.The total flavones 267mg/g extracted in Herba suadeae glaucae under these process conditions, purity is 84.6%.
Wherein when operating S3 step, first take the filtering residue that S2 obtains, then extract 1-2h with the EtOH Sonicate of 75%, from
The heart, filtration, the filtrate obtained merges with described supernatant a.
Example 3
S1. pluck fresh Herba suadeae glaucae stem and leaf, through over cleaning, be dried and after pulverizing, cross 80 mesh sieves, obtaining Herba suadeae glaucae powder；
S2. weighing the Herba suadeae glaucae powder 5g in S1 and add the distilled water of described Herba suadeae glaucae grain weight amount 12 times, adjusting pH value is 5.5, so
The compound enzyme (ratio of cellulase, pectase and papain is 1.7:0.9:0.3) of rear addition 0.05% and
Enzymolysis 20min under the conditions of the vacuum tightness of 200mpa, and hydrolysis temperature controls at 58 DEG C, extracts 2h, is centrifuged, is filtrated to get supernatant
Liquid a and filtering residue；
S3. the supernatant a taking S2 gained concentrates, and then adding 15 times of volumetric concentrations of concentrated solution volume is the second of 75%
Alcohol, extracts 2.5h, is centrifuged, is filtrated to get supernatant b；
S4. the supernatant b obtained by S3 concentrates, and then adds the chloroform of concentrated solution volume 1.5 times, stirs, stands,
To extraction liquid layer and supernatant layer, obtain total flavones crude product through isolated supernatant c, concentrated supernatant c, lyophilization；
S5. the total flavones crude product obtained by S4, dissolves with distilled water, then crosses macroporous resin column (D101-B), uses volume
Concentration is that 70% ethanol carries out eluting, eluent flow rate 2ml/min to it, collects eluent, then eluent is concentrated,
Lyophilization obtains Herba suadeae glaucae total flavones.The total flavones 232mg/g extracted in Herba suadeae glaucae under these process conditions, purity is 82.6%.
Example 4
S1. pluck fresh Herba suadeae glaucae stem and leaf, through over cleaning, be dried and after pulverizing, cross 40 mesh sieves, obtaining Herba suadeae glaucae powder；
S2. weighing the Herba suadeae glaucae powder 5g in S1 and add the distilled water of described Herba suadeae glaucae grain weight amount 12 times, adjusting pH value is 5.5, so
The compound enzyme (ratio of cellulase, pectase and papain is 1.3:0.8:0.3) of rear addition 0.05% and
Enzymolysis 25min under the conditions of the vacuum tightness of 300mpa, and hydrolysis temperature controls at 60 DEG C, extracts 2h, is centrifuged, is filtrated to get supernatant
Liquid a and filtering residue；
S3. the supernatant a taking S2 gained concentrates, and then adds the second that volumetric concentration is 50% of concentrated solution volume 18 times
Alcohol, extracts 3h, is centrifuged, is filtrated to get supernatant b；
S4. the supernatant b obtained by S3 concentrates, and then adds the ethyl acetate of concentrated solution volume 2 times, stirs, stands,
Being extracted liquid layer and supernatant layer, through isolated supernatant c, concentrated supernatant c, that lyophilization obtains total flavones is thick
Product；
S5. the total flavones crude product obtained by S4, dissolves with distilled water, then crosses macroporous resin column (D101-B), uses volume
Concentration is that 75% ethanol carries out eluting, eluent flow rate 1.5ml/min to it, collects eluent, then carries out dense to eluent
Contracting, lyophilization obtain Herba suadeae glaucae total flavones.The total flavones 212mg/g extracted in Herba suadeae glaucae under these process conditions, purity is
81.2%.
The foregoing is only presently preferred embodiments of the present invention, not in order to limit the present invention, all essences in the present invention
Any amendment, equivalent and the improvement etc. made within god and principle, should be included within the scope of the present invention.
Claims (9)
1. the extracting method of a total Flavonoids from Suaeda salsa, it is characterised in that described extracting method comprises the following steps:
S1. pluck fresh Herba suadeae glaucae stem and leaf, through over cleaning, be dried and after pulverizing, cross 20-80 mesh sieve, obtaining Herba suadeae glaucae powder；
S2. weigh the Herba suadeae glaucae powder in S1 and add the distilled water of described Herba suadeae glaucae grain weight amount 10-18 times, adjust pH value between 4.8-6,
It is subsequently adding compound enzyme enzymolysis 15-25min, extraction 1-2.5h, centrifugal, filtration under the conditions of the vacuum tightness of 200-500mpa
Obtain supernatant a and filtering residue；
S3. the supernatant a taking S2 gained concentrates, and the volumetric concentration then adding concentrated solution volume 10-20 times is 45%-80%
Ethanol, extract 1-3h, be centrifuged, be filtrated to get supernatant b；
S4. the supernatant b obtained by S3 concentrates, and then adds the extract of concentrated solution volume 1-2 times, stirs, stands, obtain
Extraction liquid layer and supernatant layer, obtain total flavones crude product through isolated supernatant c, concentrated supernatant c, lyophilization；
S5. the total flavones crude product obtained by S4, dissolves with distilled water, then crosses macroporous resin column, and is 50%-by volumetric concentration
The ethanol of 75% carries out eluting to it, collects eluent, then eluent concentration, lyophilization is obtained refined total flavonoids.
The extracting method of a kind of total Flavonoids from Suaeda salsa the most according to claim 1, it is characterised in that: described compound enzyme
Addition is the 0.03%-0.05% of described Herba suadeae glaucae grain weight amount.
The extracting method of a kind of total Flavonoids from Suaeda salsa the most according to claim 1, it is characterised in that: described compound enzyme is
Cellulase, pectase and papain, and the ratio of described cellulase, pectase and papain is 1.1-1.7:
0.7-1:0.3。
The extracting method of a kind of total Flavonoids from Suaeda salsa the most according to claim 1, it is characterised in that: the enzyme of described enzymolysis
Solve temperature and be 40-65 DEG C.
The extracting method of a kind of total Flavonoids from Suaeda salsa the most according to claim 1, it is characterised in that: take the filter that S2 obtains
Slag, then extracts 1-2h with the EtOH Sonicate of 75%, and centrifugal, filtration, the filtrate obtained merges with described supernatant a.
The extracting method of a kind of total Flavonoids from Suaeda salsa the most according to claim 1, it is characterised in that: the second in described S3
The concentration of alcohol is 65%.
The extracting method of a kind of total Flavonoids from Suaeda salsa the most according to claim 1, it is characterised in that: big in described S5
Hole resin column selects HPD-722 or D101-B.
The extracting method of a kind of total Flavonoids from Suaeda salsa the most according to claim 1, it is characterised in that: the extraction in described S4
Taking liquid is the one in petroleum ether, chloroform, ethyl acetate, n-butyl alcohol；Or ethyl acetate and the mixed liquor of n-butyl alcohol.
The extracting method of a kind of total Flavonoids from Suaeda salsa the most according to claim 8, it is characterised in that: the extraction in described S4
Take the mixed liquor that liquid is ethyl acetate and n-butyl alcohol, and described ethyl acetate mixes according to the ratio of 1:1 with n-butyl alcohol.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108096301A (en) * | 2018-02-10 | 2018-06-01 | 沧州师范学院 | The device and its process of the ultrasonic-microwave collaboration extraction fluffy middle general flavone of alkali |
| CN108252143A (en) * | 2017-12-28 | 2018-07-06 | 潍坊友容实业有限公司 | A kind of method that plant salt and cellulose are extracted from Suaeda salsa |
| CN109805216A (en) * | 2017-11-22 | 2019-05-28 | 盘锦泰成创新科技研究院有限公司 | A kind of health drink and preparation method thereof with prevention liver and bladder disease function |
| CN111518858A (en) * | 2020-05-14 | 2020-08-11 | 武汉天天好生物制品有限公司 | Vegetable protein peptide and preparation method thereof |
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| CN1915335A (en) * | 2006-07-31 | 2007-02-21 | 徐州师范大学 | General flavone of root of lilac daphne, method of extraction, and application |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109805216A (en) * | 2017-11-22 | 2019-05-28 | 盘锦泰成创新科技研究院有限公司 | A kind of health drink and preparation method thereof with prevention liver and bladder disease function |
| CN108252143A (en) * | 2017-12-28 | 2018-07-06 | 潍坊友容实业有限公司 | A kind of method that plant salt and cellulose are extracted from Suaeda salsa |
| CN108252143B (en) * | 2017-12-28 | 2020-08-21 | 潍坊友容实业有限公司 | Method for extracting plant salt and cellulose from suaeda salsa |
| CN108096301A (en) * | 2018-02-10 | 2018-06-01 | 沧州师范学院 | The device and its process of the ultrasonic-microwave collaboration extraction fluffy middle general flavone of alkali |
| CN108096301B (en) * | 2018-02-10 | 2023-12-29 | 沧州师范学院 | Device for extracting total flavonoids in suaeda salsa by ultrasonic wave-microwave cooperation and process method thereof |
| CN111518858A (en) * | 2020-05-14 | 2020-08-11 | 武汉天天好生物制品有限公司 | Vegetable protein peptide and preparation method thereof |
| CN114903927A (en) * | 2022-05-09 | 2022-08-16 | 盐城师范学院 | Medicine for preventing and treating hepatic fibrosis and preparation method thereof |
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Application publication date: 20161116 |