CN105154252B - A kind of ginkgo biloba p.e and its extracting method and application - Google Patents
A kind of ginkgo biloba p.e and its extracting method and application Download PDFInfo
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Abstract
The invention provides a kind of ginkgo biloba p.e and its extracting method and application.The extracting method is：1) ginkgo leaf is dried, crushed；2) Ginkgo Leaf is taken, adds the sodium acetate acetic acid buffer solution of pH4.0~5.5, adds cellulase, 1~2h is digested in 50~60 DEG C of water-bath；3) absolute ethyl alcohol is added, Microwave Extraction is carried out in microwave reactor, it is 50~300W to control microwave power, and 50~80 DEG C of microwave temperature, it is 1~8min to add up the microwave time；4) filter, filtrate goes the removal of impurity with petroleum ether extraction, the rotary evaporation concentration of water intaking layer, drying, produces.Cleaning product provided by the invention, due to containing ginko leaves flavone class extract, the glycosidic bond in the supplied for electronic function synergic natural washing product of the phenolic hydroxyl group of Flavonoid substances, strengthening its function of supplying power, its water-soluble ability can be increased to a certain degree, then strengthen its dirt-removing power.
Description
Technical field
The present invention relates to effective ingredients in plant, specifically, is related to a kind of ginkgo biloba p.e and its extracting method with answering
With.
Background technology
Ginkgo leaf is one of focus of recent domestic research and development, its extract (Ginkgobilobaextracts,
GBE) it is widely used in cardiovascular and cerebrovascular disease, numerous studies show, preventive and therapeutic effect of the ginkgo biloba p.e to cardiovascular and cerebrovascular disease
With its antioxidation activity Mi Guan.Main physiologically active ingredient is flavone compound and ginkgoterpene lactone class in GBE, wherein silver
Flavone, which has, removes the multiple functions such as free radical, anti-oxidant, improvement cardiovascular circulation, antifatigue, reducing blood lipid, antibacterial, silver
The purity of flavone compound is applied to play decisive action in apricot leaf, i.e., is made up of ginkgo biloba p.e for preventing and treating heart and brain
The medicine of vascular diseases requires that general flavone purity is more than 24% (the EGB761 works that generally German W.Svhwabe companies are produced
For the standardized extract of ginkgo leaf, contain 24% chromocor compound in this extract), GINKGO BILOBA EXTRACT can also be used as food
The composition of product additive, it is fabricated to antifatigue, anti-aging health food.The extraction of Flavones from Ginkgo Leaves is early in 70
Age, Germany just applied for the patent of ginkgo biloba p.e with France, from organic solvent extractions such as conventional alcohol, ketone in recent years
The supercritical fluid extraction come, high-purity and in high yield and environmentally friendly extracting method is always what people were pursued.
Gingkgo flavonoidses structure has free phenolic hydroxyl group more, is typically soluble in polarity larger organic solvent and alkali
In property solution；In ginkgo leaf most of flavones be all in the form of the larger flavonoid glycoside of polarity exist, the big flavonoid glycoside of polarity and
The larger flavone aglycone of polarity can use the larger mixed solvent of methanol, ethanol, acetone isopolarity if alcohol-water is according to certain
Ratio is extracted.
Most of current Extracting Gingko Flavonoid Glycosides are extracted using simple solvent.Such as Publication No. CN101327228
Chinese patent application disclose a kind of low-acid gingko flavones and its extracting method, add ethanol immersion, reclaim after ethanol through tree
Fat goes out lipid and then by purifying to obtain product.Publication No. CN101134767 Chinese patent application discloses a kind of height
Purity gingkgo flavonoidses high efficiency extraction preparation method, add the lower extraction Gingko yellow ketoside of alcohol at normal temperature stirring, Ran Houyong
The macroreticular resin ethanol elution of DM~130.Above-mentioned technical proposal is although simple to operate, but causes a large amount of waste of solvent, especially
It is extracting cycle length, is unfavorable for the energy that economizes on resources.
Publication No. CN102805755A Chinese patent application discloses one kind and high-quality ginkgo is extracted from ginkgo leaf
The preparation method of flavones.Crude extract is extracted from ginkgo leaf with deionized water, alcohol precipitation removal of impurities after filtering, fining agent removal of impurities, richness
Collect GINKGO BILOBA EXTRACT and lactone, using the macroreticular resin of polarity, low pole just separate.The technical scheme is extracted using water, though
Solvent cost is so saved, but further part is used alcohol precipitation and cleaned successively, high-quality fining agent, GINKGO BILOBA EXTRACT extracts not in addition
Fully, the less flavone aglycone composition of polarity in ginkgo leaf can not still be extracted.
In addition, natural washing articles for use often have the shortcomings of dissolubility is poor, and dirt-removing power is small compared to chemical washing product,
Main cause is washing of the natural washing articles for use such as using sapindoside, natural Gleditisia sinensis Saponin and Tea Saponin as principle active component
Effective water-soluble functional group of articles for use is glycoside non-ionic group, such group due to can not ionize out in aqueous from
Son, and molecular weight is larger, have impact on its water solubility, then influences its dirt-removing power.
Utilization for ginko leaves flavone, it is at present to utilize its oxidation resistant function as food additives or be used for more
In cosmeceutical.Ginkgo biloba succi is added medicine cosmetics by the Chinese patent application such as Publication No. CN101658478A
In, it was demonstrated that it has delay skin aging and reduces the functions such as wrinkle；Publication No. CN102813683 Chinese patent application is adopted
Obtaining product with Microwave Extraction ginkgo leaf has the significant function of anti-senescence function, is applied in cosmetics；Publication No.
Ginkgo leaf extract and cloves extract solution etc. are configured to moisturizing, moisturizing and suppression by CN103655293A Chinese patent application
The gingko skin care lotion of bacterium and other effects.But the research of saponin or saponin(e dirt-removing power can be strengthened so far for ginko leaves flavone
Have no report.
The content of the invention
In order to solve problems of the prior art, it is an object of the invention to provide a kind of ginkgo biloba p.e and its carry
Take method and application.
In order to realize the object of the invention, present invention firstly provides a kind of ginkgo biloba p.e, its extracting method includes as follows
Step：
1) ginkgo leaf is dried, crushed；
2) Ginkgo Leaf is taken, adds the sodium acetate-acetic acid cushioning liquid of pH4.0~5.5, cellulase is added, in 50
1~2h is digested in~60 DEG C of water-bath；
3) absolute ethyl alcohol is added, Microwave Extraction is carried out in microwave reactor, it is 50~300W to control microwave power, microwave
50~80 DEG C of temperature, it is 1~8min to add up the microwave time；
4) filter, filtrate goes the removal of impurity with petroleum ether extraction, the rotary evaporation concentration of water intaking layer, drying, produces.
Preferably, the step 1) is specially by ginkgo leaf impurity elimination, 60 DEG C are dried to constant weight in baking oven, crush, and use
60 mesh standard sieves sieve.
Preferably, the step 2) takes Ginkgo Leaf, pH4.8 sodium acetate-acetic acid cushioning liquid is added, is added fine
Plain enzyme is tieed up, 1h is digested in 50 DEG C of water-bath.
Preferably, the step 3) adds absolute ethyl alcohol, Microwave Extraction is carried out in microwave reactor, controls microwave work(
Rate is 130W, and 78 DEG C of microwave temperature, it is 4min to add up the microwave time.
Further, after adding absolute ethyl alcohol, the volume fraction of ethanol is 50~80% in system.
Further, after adding absolute ethyl alcohol, the solid-liquid ratio of Ginkgo Leaf and solution system is 1:5~1:50.
Present invention also offers a kind of extracting method of ginkgo biloba p.e, methods described comprises the following steps：
1) ginkgo leaf is dried, crushed；
2) Ginkgo Leaf is taken, adds the sodium acetate-acetic acid cushioning liquid of pH4.0~5.5, cellulase is added, in 50
1~2h is digested in~60 DEG C of water-bath；
3) absolute ethyl alcohol is added, Microwave Extraction is carried out in microwave reactor, it is 50~300W to control microwave power, microwave
50~80 DEG C of temperature, it is 1~8min to add up the microwave time；
4) filter, filtrate goes the removal of impurity with petroleum ether extraction, the rotary evaporation concentration of water intaking layer, drying, produces.
Preferably, the step 2) takes Ginkgo Leaf, pH4.8 sodium acetate-acetic acid cushioning liquid is added, is added fine
Plain enzyme is tieed up, 1h is digested in 50 DEG C of water-bath；
The step 3) adds absolute ethyl alcohol, and the volume fraction for making ethanol in system is 50~80%, in microwave reactor
Middle carry out Microwave Extraction, it is 130W to control microwave power, and 78 DEG C of microwave temperature, it is 4min to add up the microwave time.
Present invention also offers the cleaning product containing the ginkgo biloba p.e.
Further, the cleaning product contains saponin(e and/or saponin.
Present invention also offers application of the ginkgo biloba p.e in cleaning product is prepared.
The beneficial effects of the present invention are：
The present invention makes full use of the etoh solvent aqueous solution to extract most of flavone component, in combination with microwave
It is rapidly and efficiently the characteristics of, not only cost-effective, and the time is saved, by microwave power, microwave temperature and microwave time
Limit, extract most compositions of required ginko leaves flavone easily and fast.
Cleaning product provided by the invention, due to added with ginko leaves flavone class extract, the phenolic hydroxyl group of Flavonoid substances
Supplied for electronic function synergic natural washing product in glycosidic bond, strengthen its function of supplying power, its water-soluble energy can be increased to a certain degree
Power, then strengthen its dirt-removing power.
Brief description of the drawings
Fig. 1 is absorbance-concentration curve described in the embodiment of the present invention 1.
Embodiment
Following examples are used to illustrate the present invention, but are not limited to the scope of the present invention.
The ginkgo biloba p.e of embodiment 1 and its extracting method
By ginkgo leaf impurity elimination, 60 DEG C are dried to constant weight in baking oven, crush, and are sieved with 60 mesh standard sieves.Weigh ginkgo leaf
Powder 10.000g, the sodium acetate-acetic acid cushioning liquid 60mL that PH is 4.8 is added, add 0.510g cellulases and be allowed to enzyme concentration
Respectively 8.5g/L, after digesting 1h in 50 DEG C of water-bath, adding 140mL absolute ethyl alcohols makes system be 70% ethanol solution, Yu Wei
Microwave power 100W in computer microwave reactor, 75 DEG C of microwave temperature, it is 4min to add up the microwave time.
Filter, filtrate removes chlorophyll and lipid, water intaking layer rotary evaporation concentration, in baking oven 2 times with petroleum ether extraction
Drying, obtains product.
With the recovery rate of ginkgo leaf total flavonoid in ultraviolet-spectrophotometer measurement product, measuring method is：Accurately weigh silver
Apricot leaf extract 0.200g, dissolved and be settled in 100mL volumetric flasks with 75% ethanol, accurate absorption 1.0mL coarse extraction is molten
Liquid is placed in 25mL volumetric flasks, is added 5% sodium nitrite in aqueous solution 1.0mL, is shaken up, and stands 8min；Add 10% aluminum nitrate
Aqueous solution 1.0mL, shakes up, and stands 8min；4% sodium hydrate aqueous solution 10.0mL is added, adds 75% ethanol solution constant volume
To 25mL, 30min is stood, goes out to measure absorbance with 510nm.Absorbance-concentration curve is shown in Fig. 1.And by absorbance according to standard
Curve finds concentration C mg/mL, then content and recovery rate have below equation to calculate in extract：
Extractive content=[(C × 25 × 100)/(0.200 × 1000)] × 100%
Recovery rate=[(crude extract gross mass × extractive content)/ginkgo leaf weight] × 100%
Recovery rate is 2.80%, extractive content 9.50%.
The ginkgo biloba p.e of embodiment 2 and its extracting method
By ginkgo leaf impurity elimination, 60 DEG C are dried to constant weight in baking oven, crush, and are sieved with 60 mesh standard sieves.Take ginkgo leaf powder
Last 10.000g, the cushioning liquid 60mL that pH is respectively 4.8 is added, it is 8.5g/L to add 0.510g cellulases and be allowed to enzyme concentration,
After digesting 1h in 50 DEG C of water-bath, adding 140mL absolute ethyl alcohols makes system be 70% ethanol solution, in micro computer microwave reactor
Middle power 80W, 70 DEG C of microwave temperature, it is respectively 2min to add up the microwave time.
Filter, filtrate removes chlorophyll and lipid, water intaking layer rotary evaporation concentration, in baking oven 2 times with petroleum ether extraction
Drying, obtains product.
With the recovery rate of ginkgo leaf total flavonoid in ultraviolet-spectrophotometer measurement product, measuring method is surveyed with embodiment 1
It is 2.53% to obtain recovery rate, extractive content 9.32%.
The ginkgo biloba p.e of embodiment 3 and its extracting method
By ginkgo leaf impurity elimination, 60 DEG C are dried to constant weight in baking oven, crush, and are sieved with 60 mesh standard sieves.Weigh ginkgo leaf
Powder 10.000g, the cushioning liquid 60mL that pH is respectively 4.8 is added, it is 8.5g/ to add 0.510g cellulases and be allowed to enzyme concentration
L, after digesting 1h in 50 DEG C of water-bath, adding 140mL absolute ethyl alcohols makes system be 70% ethanol solution, in micro computer microwave reaction
Microwave power 130W in device, 78 DEG C of microwave temperature, it is respectively 4min to add up the microwave time.
Filter, filtrate removes chlorophyll and lipid, water intaking layer rotary evaporation concentration, in baking oven 2 times with petroleum ether extraction
Drying, obtains product.
With the recovery rate of ginkgo leaf total flavonoid in ultraviolet-spectrophotometer measurement product, measuring method is surveyed with embodiment 1
It is 3.96% to obtain recovery rate, extractive content 10.34%.
The ginkgo biloba p.e of embodiment 4 and its extracting method
By ginkgo leaf impurity elimination, 60 DEG C are dried to constant weight in baking oven, crush, and are sieved with 60 mesh standard sieves.Weigh ginkgo leaf
Powder 10.000g, the sodium acetate-acetic acid cushioning liquid 60mL that pH is 4.8 is added, add 0.454g cellulases and be allowed to enzyme concentration
After respectively 7.56g/L digests 2h in 50 DEG C of water-bath, adding 140mL absolute ethyl alcohols makes system be 70% ethanol solution, Yu Wei
Microwave power 150W in computer microwave reactor, 80 DEG C of microwave temperature, it is 4min to add up the microwave time.
Filter, filtrate removes chlorophyll and lipid, water intaking layer rotary evaporation concentration, in baking oven 2 times with petroleum ether extraction
Drying, obtains product.
With the recovery rate of ginkgo leaf total flavonoid in ultraviolet-spectrophotometer measurement product, measuring method carries with embodiment 1
It is 3.46% to take rate, extractive content 9.76%.
The ginkgo biloba p.e of embodiment 5 and its extracting method
By ginkgo leaf impurity elimination, 60 DEG C are dried to constant weight in baking oven, crush, and are sieved with 60 mesh standard sieves.Weigh ginkgo leaf
Powder 10.000g, the sodium acetate-acetic acid cushioning liquid 60mL that pH is 4.8 is added, add 0.510g cellulases and be allowed to enzyme concentration
For 8.5g/L, after 1h is digested in 60 DEG C of water-bath, adding 140mL absolute ethyl alcohols makes system be 70% ethanol solution, Yu Wei
Microwave power 150W in computer microwave reactor, 65 DEG C of microwave temperature, it is 4min to add up the microwave time.
Filter, filtrate removes chlorophyll and lipid, water intaking layer rotary evaporation concentration, in baking oven 2 times with petroleum ether extraction
Drying, obtains product.
With the recovery rate of ginkgo leaf total flavonoid in ultraviolet-spectrophotometer measurement product, measuring method carries with embodiment 1
It is 4.00% to take rate, extractive content 8.14%.
The ginkgo biloba p.e of embodiment 6 and its extracting method
By ginkgo leaf impurity elimination, 60 DEG C are dried to constant weight in baking oven, crush, and are sieved with 60 mesh standard sieves.Weigh ginkgo leaf
Powder 10.000g, the sodium acetate-acetic acid cushioning liquid 60mL that pH is 4.5 is added, add 0.454g cellulases and be allowed to enzyme concentration
After respectively 7.56g/L digests 2h in 50 DEG C of water-bath, adding 140mL absolute ethyl alcohols makes system be 70% ethanol solution, Yu Wei
Microwave power 300W in computer microwave reactor, 80 DEG C of microwave temperature, it is 4min to add up the microwave time.
Filter, filtrate removes chlorophyll and lipid, water intaking layer rotary evaporation concentration, in baking oven 2 times with petroleum ether extraction
Drying, obtains product.
With the recovery rate of ginkgo leaf total flavonoid in ultraviolet-spectrophotometer measurement product, measuring method carries with embodiment 1
It is 3.12% to take rate, extractive content 8.92%.
The ginkgo biloba p.e of embodiment 7 and its extracting method
By ginkgo leaf impurity elimination, 60 DEG C are dried to constant weight in baking oven, crush, and are sieved with 60 mesh standard sieves.Weigh ginkgo leaf
Powder 10.000g, the sodium acetate-acetic acid cushioning liquid 60mL that pH is 4.8 is added, add 0.454g cellulases and be allowed to enzyme concentration
After respectively 7.56g/L digests 2h in 50 DEG C of water-bath, adding 60mL absolute ethyl alcohols makes system be 50% ethanol solution, Yu Wei electricity
Microwave power 150W in brain microwave reactor, 80 DEG C of microwave temperature, it is 4min to add up the microwave time.
Filter, filtrate removes chlorophyll and lipid, water intaking layer rotary evaporation concentration, in baking oven 2 times with petroleum ether extraction
Drying, obtains product.
With the recovery rate of ginkgo leaf total flavonoid in ultraviolet-spectrophotometer measurement product, measuring method embodiment 1, extraction
Rate is 3.00%, extractive content 7.46%.
The ginkgo biloba p.e of embodiment 8 and its extracting method
By ginkgo leaf impurity elimination, 60 DEG C are dried to constant weight in baking oven, crush, and are sieved with 60 mesh standard sieves.Weigh ginkgo leaf
Powder 10.000g, the sodium acetate-acetic acid cushioning liquid 60mL that pH is 4.8 is added, add 0.454g cellulases and be allowed to enzyme concentration
After respectively 7.56g/L digests 2h in 50 DEG C of water-bath, adding 240mL absolute ethyl alcohols makes system be 80% ethanol solution, Yu Wei
Microwave power 150W in computer microwave reactor, 80 DEG C of microwave temperature, it is 4min to add up the microwave time.
Filter, filtrate removes chlorophyll and lipid, water intaking layer rotary evaporation concentration, in baking oven 2 times with petroleum ether extraction
Drying, obtains product.
With the recovery rate of ginkgo leaf total flavonoid in ultraviolet-spectrophotometer measurement product, measuring method carries with embodiment 1
It is 3.80% to take rate, extractive content 8.96%.
Comparative example 1
Ginkgo Leaf 10.000g is taken, adding 140mL absolute ethyl alcohols makes system be 70% ethanol solution, micro- in micro computer
Power 80W in ripple reactor, 70 DEG C of microwave temperature, accumulative microwave time are respectively that 2min recovery rates are 1.50%, and extract contains
Measure as 4.52%.
Comparative example 2
Ginkgo Leaf 10.000g is weighed, adds the cushioning liquid 60mL that pH is respectively 4.8, adds 0.510g celluloses
It is 8.50g/L that enzyme, which is allowed to enzyme concentration, and after digesting 1h in 50 DEG C of water-bath, adding 140mL absolute ethyl alcohols makes system be 70% ethanol
Solution, the microwave power 130W in micro computer microwave reactor, 38 DEG C of microwave temperature, it is respectively 6min to add up the microwave time, is carried
It is 1.40% to take rate, extractive content 6.32%.
Comparative example 3
By ginkgo leaf impurity elimination, 60 DEG C are dried to constant weight in baking oven, crush, and are sieved with 60 mesh standard sieves.Weigh ginkgo leaf
Powder 10.000g, the sodium acetate-acetic acid cushioning liquid 60mL that pH is 4.8 is added, add 0.454g cellulases and be allowed to enzyme concentration
After respectively 7.56g/L digests 2h in 50 DEG C of water-bath, adding 140mL absolute ethyl alcohols makes system be 70% ethanol solution, Yu Wei
Microwave power 150W in computer microwave reactor, 80 DEG C of microwave temperature, it is 15min to add up the microwave time, is filtered, filtrate oil
Ether extracts 2 times and removes green element and lipid, the rotary evaporation concentration of water intaking layer, is dried in baking oven, claims crude extract quality, determine extinction
Degree, recovery rate 2.05%, extractive content 5.43%.
Comparative example 4
By ginkgo leaf impurity elimination, 60 DEG C are dried to constant weight in baking oven, crush, and are sieved with 60 mesh standard sieves.Weigh ginkgo leaf
Powder 10.000g, the sodium acetate-acetic acid cushioning liquid 60mL that pH is 4.8 is added, add 0.510g cellulases and be allowed to enzyme concentration
For 8.50g/L, after 1h is digested in 60 DEG C of water-bath, adding 140mL absolute ethyl alcohols makes system be 70% ethanol solution, Yu Wei
Microwave power 30W in computer microwave reactor, 65 DEG C of microwave temperature, it is 4min to add up the microwave time, is filtered, filtrate petroleum ether
Extract 2 times and remove chlorophyll and lipid, the rotary evaporation concentration of water intaking layer, dried in baking oven, claim crude extract quality, determine extinction
Degree, recovery rate 1.40%, extractive content 3.14%.
Articles for washing of the embodiment 9 containing ginkgo biloba p.e
Ginkgo biloba p.e 0.4g described in embodiment 1, vitamin C 0.4g, sapindoside 100g, sandalwood essential oil 0.2g,
Water purification 1Kg, 45 DEG C of heating stir, cooling.
Dirt-removing power is tested referring to GB/T13174~2008, test result：Decontamination value R85.8, can arbitrarily it be dissolved in cold
Water.
Articles for washing of the embodiment 10 containing ginkgo biloba p.e
Sodium hydroxide 80g, pure water 300g are heated with stirring to 50 DEG C of dissolvings, add Gleditisia sinensis Saponin 100g, described in embodiment 1
Ginkgo biloba p.e 0.8g, olive oil 100g, stir 60min, and powder is made in spray-dried and cyclone separator.
Dirt-removing power is tested referring to GB/T13174~2008, test result：Decontamination value R73.8,10g/100g hydroecium temperature
It can dissolve.
Articles for washing of the embodiment 11 containing ginkgo biloba p.e
Ginkgo biloba p.e 0.3g described in embodiment 1, Tea Saponin 40g, sapindoside 100g, propane diols 5g, glycerol liquor
Solve soybean protein 1g, Ginseng extract 0.2g, hydrolytic soya bean protein 10g essence 0.1g, methyl hydroxybenzoate 5g, pure water 3kg, 55 DEG C
Stir, cooling.
Dirt-removing power is tested referring to GB/T13174~2008, test result：Decontamination value R75.6,10g/50g hydroecium temperature
It can dissolve.
Comparative example 5
Vitamin C 0.4g, 50~100g of sapindoside, sandalwood essential oil 0.2g, water purification 1Kg, 45 DEG C of heating stir,
Cooling.Dirt-removing power is tested referring to GB/T13174~2008, test result：Decontamination value 69.0,50 DEG C of temperature of 30g/50g
Water dissolvable.
Comparative example 6
Tea Saponin 40g, sapindoside 100g, propane diols 5g, glycerine hydrolysis mostly albumen 1g, Ginseng extract 0.2g,
Hydrolytic soya bean protein 10g essence 0.1g, methyl hydroxybenzoate 5g, pure water 3kg, 55 DEG C stir, cooling.Dirt-removing power is surveyed
Examination referring to GB/T13174~2008, test result is：45 DEG C of decontamination value R70.9,10g/50g water can dissolve.
Although above the present invention is described in detail with a general description of the specific embodiments,
On the basis of the present invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Cause
This, these modifications or improvements, belong to the scope of protection of present invention without departing from theon the basis of the spirit of the present invention.
Claims (3)
1. a kind of extracting method of ginkgo biloba p.e, it is characterised in that methods described comprises the following steps：
1) ginkgo leaf is dried, crushed；
2) Ginkgo Leaf is taken, adds the sodium acetate-acetic acid cushioning liquid of pH4.0~5.5, cellulase is added, in 50~60
DEG C water-bath in digest 1~2h；
3) absolute ethyl alcohol is added, the volume fraction for making ethanol in system is 50~80%, and microwave is carried out in microwave reactor and is carried
Take, it is 130W to control microwave power, and 78 DEG C of microwave temperature, it is 4min to add up the microwave time；
4) filter, filtrate goes the removal of impurity with petroleum ether extraction, the rotary evaporation concentration of water intaking layer, drying, produces.
2. according to the method for claim 1, it is characterised in that the step 2) takes Ginkgo Leaf, adds pH4.8 second
Sour sodium-acetic acid buffer solution, cellulase is added, 1h is digested in 50 DEG C of water-bath.
3. the ginkgo biloba p.e that the methods described of claim 1 or 2 is prepared is in enhancing natural washing product dirt-removing power side
The application in face.
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| CN107751557A (en) * | 2016-08-22 | 2018-03-06 | 北京大伟嘉生物技术股份有限公司 | A kind of Chinese herb feed additive for reducing egg cholesterol and its application |
| CN106520858B (en) * | 2016-10-20 | 2019-12-06 | 徐州工程学院 | extraction method of ginkgo leaf water-soluble dietary fiber |
| CN107019712A (en) * | 2017-05-19 | 2017-08-08 | 临沂大学 | A kind of extraction process of ginkgo leaf |
| CN114907896A (en) * | 2022-04-27 | 2022-08-16 | 南京林业大学 | Ginkgo leaf enzymolysis treatment method and application thereof |
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