CN103340948A - Preparation method of hawthorn leaf extract - Google Patents
Preparation method of hawthorn leaf extract Download PDFInfo
- Publication number
- CN103340948A CN103340948A CN2013103011304A CN201310301130A CN103340948A CN 103340948 A CN103340948 A CN 103340948A CN 2013103011304 A CN2013103011304 A CN 2013103011304A CN 201310301130 A CN201310301130 A CN 201310301130A CN 103340948 A CN103340948 A CN 103340948A
- Authority
- CN
- China
- Prior art keywords
- ethanol
- extraction
- preparation
- crude product
- leaves
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The invention provides a preparation method of a hawthorn leaf extract. The preparation method comprises the following steps of: grinding hawthorn leaves, soaking the ground leaves for 1 hour by 70% of ethanol under the condition that the ratio of the ground leaves to ethanol is 1 to 7, extracting the soaked leaves for 45 minutes by an ultrasonic grinding machine with ultrasonic power of 150W and frequency of 80HZ, centrifuging an extracting solution for 15 minutes at speed of 4000r/min, washing residues by a small amount of 70% of ethanol, centrifuging for 5 minutes, combining centrifugal solutions, performing reflux extraction for 1 hour, filtering, performing decompression concentration on filter liquor to obtain a crude product, dissolving the crude product in 95% of ethanol, filtering, performing reduced-pressure distillation, adding 95% of ethanol and ethyl acetate in sequence, extracting for multiple times, and refining. Ultrasound is supplemented in a conventional extraction process, so that cell walls can be destroyed, effective ingredients can be conveniently extracted, and the product yield can be increased; further, a physical process does not enable the effective ingredients to be destroyed and can yet be regarded as an effective way for extraction.
Description
Technical field
The present invention relates to the extracting method of a kind of plant, be specifically related to a kind of extracting method of Folium Crataegi extract.
Background technology
Folium Crataegi total flavones is Rosaceae Crataegus plant Fructus Pyri Pashiae, the total flavones that the dried leaves of CrataegusPinnatifidaBge.VarMajorN.E.BR. or Fructus Crataegi CrataegusPinnatifidaBge. is extracted.Main Ingredients and Appearance have vitexin rhamnopyranoside, hyperin and low before paracyanogen fixed etc.Have blood pressure lowering, increase pharmacological actions such as coronary flow, blood fat reducing, heart tonifying, anti-arrhythmia.Acute toxicity test shows: the oral Folium Crataegi total flavones maximum tolerated dose of mice (be equivalent to clinical consumption 111 times) more than 9.8g/kg, namely this product has no side effect and untoward reaction.
At present, the method that Folium Crataegi extracts flavone is a lot, comprises water extraction, alcohol extracting method, macroporous adsorbent resin method.
The extracting method of 200410093893.5 1 kinds of Folium Crataegi of application number concentrates the back aqueous precipitation with the Folium Crataegi ethanol extract, crosses macroporous resin column, wash the water-ethanol eluting again with water, the concentrated spray drying, the Fructus Crataegi extract total flavones amount that this method obtains contains quantity not sufficient 60%, yield less than 1%.
The preparation method of the preparation method Folium Crataegi extract of application number 201110325953.1 Folium Crataegi extracts, polyamide resin column was listened in this invention, carried out eluting with deionized water, and decompression recycling ethanol obtains Folium Crataegi extract.The Folium Crataegi extract of the inventive method preparation, general flavone content is greater than 90%, and complicated process of preparation, yield maximum only are 2.34%.
Summary of the invention
The present invention is for addressing the above problem, and a kind of yield height, technology is simple, content is high extraction process are provided.
A kind of preparation method of Folium Crataegi extract is as follows:
Folium Crataegi is pulverized, the soak with ethanol with 70% 1 hour, and solid-liquid ratio is 1:7, be 150W with ultrasonic power, frequency is the ultrasonic grinding machine extraction 45min of 80HZ, extracting solution centrifugal (4000r/min) 15min, with a spot of 70% washing with alcohol residue, centrifugal 5min merges centrifugal liquid, reflux, extract, 1h again, filter, filtrate decompression concentrates, and gets crude product, crude product is dissolved in 95% ethanol, filter, distilling under reduced pressure adds 95% ethanol, ethyl acetate extraction successively and repeatedly makes with extra care.
After deliberation, the solvent that extraction is used and the number of times of extraction are very big to the content influence of product, so the extraction conditions that the present invention selects for use is 95% alcohol extraction 2 times, and ethyl acetate extraction 2 times.
Compared with prior art, advantage of the present invention is:
1) in tradition is extracted, be aided with ultrasonicly, can destroy cell wall, be beneficial to the extraction of effective ingredient, improve product yield, and this physical process can not make effective ingredient wreck, a kind of effective way of can yet be regarded as and extracting.
2) crude product is dissolved in 95% ethanol, filters, distilling under reduced pressure adds 95% ethanol, ethyl acetate extraction successively and repeatedly makes with extra care.Improved the content of extract.
Embodiment 1
Folium Crataegi 100g pulverizes, the soak with ethanol with 70% 1 hour, and solid-liquid ratio is 1:7, be 150W with ultrasonic power, frequency is the ultrasonic grinding machine extraction 45min of 80HZ, extracting solution centrifugal (4000r/min) 15min, with a spot of 70% washing with alcohol residue, centrifugal 5min merges centrifugal liquid, reflux, extract, 1h again, filter, filtrate decompression concentrates, and gets crude product, crude product is dissolved in 95% ethanol, filter, distilling under reduced pressure, add 95% ethanol successively 2 times, ethyl acetate extraction 2 times refining extract.The amount that detects total flavones with ultraviolet spectrophotometry is 98.4%, yield 4.5%.
Embodiment 2
Folium Crataegi 100g pulverizes, the soak with ethanol with 70% 1 hour, and solid-liquid ratio is 1:7, be 150W with ultrasonic power, frequency is the ultrasonic grinding machine extraction 45min of 80HZ, extracting solution centrifugal (4000r/min) 15min, with a spot of 70% washing with alcohol residue, centrifugal 5min merges centrifugal liquid, reflux, extract, 1h again, filter, filtrate decompression concentrates, and gets crude product, crude product is dissolved in 95% ethanol, filters distilling under reduced pressure, concentrate 95% alcohol extraction 1 time, refining extract.The amount that detects total flavones with ultraviolet spectrophotometry is 95.6%, yield 4.2%.
Claims (2)
1. the preparation method of a Folium Crataegi extract is characterized in that step is as follows:
Folium Crataegi is pulverized, crossed and shine, the soak with ethanol with 70% 1 hour, solid-liquid ratio is 1:7, is 150W with ultrasonic power, and frequency is the ultrasonic grinding machine extraction 45min of 80HZ, the centrifugal 15min of extracting solution 4000r/min, with a spot of 70% washing with alcohol residue, centrifugal 5min merges centrifugal liquid, reflux, extract, 1h filters again, and filtrate decompression concentrates, get crude product, crude product is dissolved in 95% ethanol, filter, distilling under reduced pressure adds 95% ethanol, ethyl acetate extraction successively and repeatedly makes with extra care.
2. according to the preparation method of the described Folium Crataegi extract of claim 1, it is characterized in that extraction conditions is 95% alcohol extraction 2 times, ethyl acetate extraction 2 times.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013103011304A CN103340948A (en) | 2013-07-18 | 2013-07-18 | Preparation method of hawthorn leaf extract |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013103011304A CN103340948A (en) | 2013-07-18 | 2013-07-18 | Preparation method of hawthorn leaf extract |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103340948A true CN103340948A (en) | 2013-10-09 |
Family
ID=49275808
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2013103011304A Withdrawn CN103340948A (en) | 2013-07-18 | 2013-07-18 | Preparation method of hawthorn leaf extract |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103340948A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105105282A (en) * | 2015-09-14 | 2015-12-02 | 青岛农业大学 | Composite coating fresh keeping agent containing hawthorn leaf flavone and preparation method and application thereof |
| CN112245481A (en) * | 2020-11-05 | 2021-01-22 | 广东广发制药有限公司 | Extraction method of hawthorn leaf extract and hawthorn leaf extract |
| CN112675228A (en) * | 2021-01-18 | 2021-04-20 | 南京邮电大学 | Ointment for promoting wound healing and preparation method thereof |
-
2013
- 2013-07-18 CN CN2013103011304A patent/CN103340948A/en not_active Withdrawn
Non-Patent Citations (1)
| Title |
|---|
| 许瑞波等: "山楂叶黄酮的超声提取、精制及抗氧化研究", 《食品研究与开发》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105105282A (en) * | 2015-09-14 | 2015-12-02 | 青岛农业大学 | Composite coating fresh keeping agent containing hawthorn leaf flavone and preparation method and application thereof |
| CN112245481A (en) * | 2020-11-05 | 2021-01-22 | 广东广发制药有限公司 | Extraction method of hawthorn leaf extract and hawthorn leaf extract |
| CN112675228A (en) * | 2021-01-18 | 2021-04-20 | 南京邮电大学 | Ointment for promoting wound healing and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104387356B (en) | In ginkgo leaf, extract the method for flavones | |
| CN104171157B (en) | A kind of green tea extract and preparation method thereof, application | |
| CN103467617A (en) | Method for continuous counter-current ultrasonic extraction of high-purity astragalus polysaccharide | |
| CN101560201A (en) | Technique for extracting high purity puerarin and diverse rare medical components from root of kudzuvine | |
| CN105154509A (en) | Extraction method of ginseng peptide | |
| CN105713058A (en) | Method for synchronously preparing chlorogenic acid and cynaroside from Lonicera japonica leaves | |
| CN103446195B (en) | The preparation method of Folium Ginkgo extract | |
| CN106361801A (en) | Cicer arietinum L. isoflavone separating and purifying method | |
| CN102349951A (en) | Preparation method of hawthorn leaf extract | |
| CN103520233A (en) | Preparation method and application of apocynum venetum leaf total polyphenol | |
| CN107126452A (en) | Gypenosides and preparation method thereof | |
| CN103304532A (en) | Method for extracting procyanidine from receptaculum nelumbinis | |
| CN104606262A (en) | Method for extracting total flavones from passiflora edulis peels | |
| CN101519408B (en) | Method for producing glabridin from glycyrrhiza residue | |
| CN103340948A (en) | Preparation method of hawthorn leaf extract | |
| CN104940280A (en) | Method for extracting total flavones from radix puerariae employing enzyme preparation | |
| CN103446246A (en) | Process for extracting effective ingredients from myrtle roots | |
| CN106581108B (en) | Method for removing residual pesticide propamocarb in ginseng extract | |
| CN104825523A (en) | Wild chrysanthemum flower general flavone extraction method | |
| CN104945450B (en) | A kind of method that Stibene-glucoside is extracted from the vine of multiflower knotweed | |
| CN102302539A (en) | Method for producing trifolium pratense L. isoflavones | |
| CN106138203A (en) | A kind of preparation method of danshen powder | |
| CN102600228B (en) | Method for preparing gypenoside in gypenoside grains | |
| CN102000125B (en) | Ginkgo flavone extraction method | |
| CN103690588A (en) | Production method for extracting refined gypenosides from gynostemma pentaphylla |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C04 | Withdrawal of patent application after publication (patent law 2001) | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20131009 |