CN105154252A - Ginkgo leaf extract, and extraction method and application thereof - Google Patents
Ginkgo leaf extract, and extraction method and application thereof Download PDFInfo
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Abstract
The invention provides a ginkgo leaf extract, and an extraction method and application thereof. The extraction method comprises the following steps: 1) drying ginkgo leaf, and pulverizing; 2) taking the ginkgo leaf powder, adding a sodium acetate-acetic acid buffer solution with the pH value of 4.0-5.5, adding cellulase, and carrying out enzymolysis in a 50-60-DEG C water bath for 1-2 hours; 3) adding anhydrous ethanol, and carrying out microwave extraction in a microwave reactor, wherein the microwave power is controlled at 50-300W, the microwave temperature is 50-80 DEG C, and the accumulative microwave time is 1-8 minutes; and 4) carrying out vacuum filtration, extracting the filtrate with petroleum ether to remove impurities, taking the water layer, concentrating the water layer by rotary evaporation, and drying. The washing product provided by the invention contains the ginkgo leaf flavone extract, and the electron donating function of the phenolic hydroxyl group of the flavones cooperates with the glycosidic bond in the natural washing product to enhance the electron donating function and enhance the water solubility to some extent, thereby enhancing the dirt-removing power.
Description
Technical field
The present invention relates to effective ingredients in plant, specifically, relate to a kind of Folium Ginkgo extract and extracting method thereof and application.
Background technology
Ginkgo Leaf is one of focus of recent domestic research and development, its extract (Ginkgobilobaextracts, GBE) be widely used in cardiovascular and cerebrovascular diseases, large quantity research shows, Folium Ginkgo extract is to the preventive and therapeutic effect of cardiovascular and cerebrovascular diseases and its anti-oxidant activity Mi Guan.Physiologically active ingredient main in GBE is flavonoid compound and Folium Ginkgo terpene lactones class, wherein ginkgolic flavone glycoside has scavenging free radicals, anti-oxidant, improve cardiovascular and cerebrovascular circulation, antifatigue, reducing blood-fat, antibacterial several functions such as grade, the purity of Flavones from Ginkgo Leaves plays decisive action to its application, namely (EGB761 usually German W.Svhwabe company produced is as the standardized extract of Ginkgo Leaf to require total flavones purity to be more than 24% by the Folium Ginkgo extract medicine made for preventing and treating cardiovascular and cerebrovascular diseases, containing the chromocor compound of 24% in this extract), ginkgolic flavone glycoside also can as the composition of foodstuff additive, be made into antifatigue, antidotal protective foods.The extraction of Flavones from Ginkgo Leaves as far back as the seventies Germany and France just applied for the patent of Folium Ginkgo extract, from the conventional organic solvent extraction such as alcohol, ketone to supercritical fluid extraction in recent years, high purity and high yield and the extracting method of environmental protection is people always pursues.
Gingkgo flavonoids structure has free phenolic hydroxyl group more, is generally soluble in the larger organic solvent of polarity and basic solution; In Ginkgo Leaf, most of flavones is all exist with the form of the larger flavonoid glycoside of polarity, and the flavonoid glycoside that polarity is large and the larger Flavone aglycone of polarity can extract according to certain ratio with methyl alcohol, ethanol, mixed solvent such as alcohol-water that acetone isopolarity is larger.
Current Extracting Gingko Flavonoid Glycosides great majority adopt simple solvent to extract.The Chinese patent application being CN101327228 as publication number discloses a kind of low-acid gingko flavones and extracting method thereof, adds alcohol immersion, reclaims to go out lipid through resin after ethanol and then obtain product through purifying.Publication number is that the Chinese patent application of CN101134767 discloses a kind of highly effective abstraction method for preparation of high-purity ginko flavonoid, adds alcohol at normal temperature and stirs lower extraction Gingko yellow ketoside, then with DM ~ 130 macroporous resin ethanol elution.Although technique scheme is simple to operate, cause a large amount of waste of solvent, especially extracting cycle is long, is unfavorable for the energy that economizes on resources.
Publication number is that the Chinese patent application of CN102805755A discloses a kind of preparation method extracting high-quality ginkgo flavone from Ginkgo Leaf.From Ginkgo Leaf, extract crude extract with deionized water, alcohol precipitation removal of impurities after filtering, finings removal of impurities, enriching ginkgetin and lactone, utilize the macroporous resin of polarity, low-pole to carry out just being separated.This technical scheme utilizes water extraction, although save solvent cost, further part uses alcohol precipitation removal of impurities successively, high-quality finings, and ginkgolic flavone glycoside extracts insufficient in addition, still can not extract the Flavone aglycone composition that Ginkgo Leaf Semi-polarity is less.
In addition, natural washing articles for use often have poorly soluble compared to chemical washing product, the shortcomings such as soil removability is little, major cause is the effective water-soluble functional group of natural washing articles for use as the articles for washing being principle active component with sapindoside, natural Gleditisia sinensis Saponin and tea saponin is glucosides class non-ionic group, such group is owing to can not ionize out ion in aqueous, and molecular weight is comparatively large, have impact on that it is water-soluble, then affect its soil removability.
For the utilization of ginko leaves flavone, at present utilize its oxidation resistant function to it can be used as foodstuff additive or in cosmeceutical more.Ginkgo biloba succi adds in medicine makeup by the Chinese patent application being CN101658478A as publication number, confirms that it has delay skin aging and reduces the functions such as wrinkle; Publication number is that the Chinese patent application of CN102813683 adopts microwave extraction Ginkgo Leaf to obtain product to have the significant function of antisenility function, be applied in makeup; Publication number is that Ginkgo Leaf extract and cloves extracting solution etc. are mixed with the gingko skin care lotion with moisturizing, moisturizing and the effect such as antibacterial by the Chinese patent application of CN103655293A.But the research that can strengthen saponin or saponin(e soil removability for ginko leaves flavone is not reported so far.
Summary of the invention
In order to solve problems of the prior art, the object of this invention is to provide a kind of Folium Ginkgo extract and extracting method thereof and application.
In order to realize the object of the invention, first the present invention provides a kind of Folium Ginkgo extract, and its extracting method comprises the steps:
1) Ginkgo Leaf is dried, pulverize;
2) get Ginkgo Leaf, add the sodium acetate-acetic acid buffered soln of pH4.0 ~ 5.5, add cellulase, enzymolysis 1 ~ 2h in the water-bath of 50 ~ 60 DEG C;
3) add dehydrated alcohol, in microwave reactor, carry out microwave extraction, control microwave power is 50 ~ 300W, microwave temperature 50 ~ 80 DEG C, and the accumulative microwave time is 1 ~ 8min;
4) suction filtration, filtrate removes impurity with petroleum ether extraction, and water intaking layer rotary evaporation concentrates, and dries, to obtain final product.
As preferably, described step 1) be specially Ginkgo Leaf impurity elimination, dry to constant weight for 60 DEG C in baking oven, pulverize, sieve with 60 mesh standard sieves.
As preferably, described step 2) get Ginkgo Leaf, add the sodium acetate-acetic acid buffered soln of pH4.8, add cellulase, enzymolysis 1h in the water-bath of 50 DEG C.
As preferably, described step 3) add dehydrated alcohol, in microwave reactor, carry out microwave extraction, control microwave power is 130W, microwave temperature 78 DEG C, and the accumulative microwave time is 4min.
Further, after adding dehydrated alcohol, in system, the volume fraction of ethanol is 50 ~ 80%.
Further, after adding dehydrated alcohol, the solid-liquid ratio of Ginkgo Leaf and solution system is 1:5 ~ 1:50.
Present invention also offers a kind of extracting method of Folium Ginkgo extract, described method comprises the steps:
1) Ginkgo Leaf is dried, pulverize;
2) get Ginkgo Leaf, add the sodium acetate-acetic acid buffered soln of pH4.0 ~ 5.5, add cellulase, enzymolysis 1 ~ 2h in the water-bath of 50 ~ 60 DEG C;
3) add dehydrated alcohol, in microwave reactor, carry out microwave extraction, control microwave power is 50 ~ 300W, microwave temperature 50 ~ 80 DEG C, and the accumulative microwave time is 1 ~ 8min;
4) suction filtration, filtrate removes impurity with petroleum ether extraction, and water intaking layer rotary evaporation concentrates, and dries, to obtain final product.
As preferably, described step 2) get Ginkgo Leaf, add the sodium acetate-acetic acid buffered soln of pH4.8, add cellulase, enzymolysis 1h in the water-bath of 50 DEG C;
Described step 3) add dehydrated alcohol, the volume fraction making ethanol in system is 50 ~ 80%, in microwave reactor, carry out microwave extraction, and control microwave power is 130W, microwave temperature 78 DEG C, and the accumulative microwave time is 4min.
Present invention also offers the cleaning product containing described Folium Ginkgo extract.
Further, described cleaning product contains saponin(e and/or saponin.
Present invention also offers the application of described Folium Ginkgo extract in preparing washing product.
Beneficial effect of the present invention is:
The present invention makes full use of the etoh solvent aqueous solution and is extracted by the flavone component of the overwhelming majority, simultaneously in conjunction with microwave feature rapidly and efficiently, not only cost-saving, and save time, by to microwave power, microwave temperature and the restriction of microwave time, extract most compositions of required ginko leaves flavone easily and fast.
Cleaning product provided by the invention, owing to being added with ginko leaves flavone class extract, the glycosidic link in the supplied for electronic function synergic natural washing product of the phenolic hydroxyl group of Flavonoid substances, strengthens its function of supplying power, to a certain degree can increase its water-soluble ability, then strengthen its soil removability.
Accompanying drawing explanation
Fig. 1 is the absorbancy-concentration curve described in the embodiment of the present invention 1.
Embodiment
Following examples for illustration of the present invention, but are not used for limiting the scope of the invention.
Embodiment 1 Folium Ginkgo extract and extracting method thereof
By Ginkgo Leaf impurity elimination, dry to constant weight for 60 DEG C in baking oven, pulverize, sieve with 60 mesh standard sieves.Take Ginkgo Leaf 10.000g, add the sodium acetate-acetic acid buffered soln 60mL that PH is 4.8, add 0.510g cellulase to make it enzyme concn and be respectively 8.5g/L, in water-bath 50 DEG C after enzymolysis 1h, adding 140mL dehydrated alcohol makes system be 70% ethanolic soln, microwave power 100W in micro computer microwave reactor, microwave temperature 75 DEG C, the accumulative microwave time is 4min.
Suction filtration, filtrate removes chlorophyll and lipid 2 times with petroleum ether extraction, and water intaking layer rotary evaporation concentrates, and dries, obtain product in baking oven.
With the extraction yield of Folium Ginkgo total flavones in ultraviolet-spectrophotometer measurement product, measuring method is: accurately take Folium Ginkgo extract 0.200g, be settled in 100mL volumetric flask with 75% dissolve with ethanol, the thick extraction solution of accurate absorption 1.0mL is placed in 25mL volumetric flask, add 5% sodium nitrite in aqueous solution 1.0mL again, shake up, leave standstill 8min; Add 10% aluminum nitrate aqueous solution 1.0mL, shake up, leave standstill 8min; Add 4% aqueous sodium hydroxide solution 10.0mL again, add 75% ethanolic soln and be settled to 25mL, leave standstill 30min, go out to measure absorbancy with 510nm.Absorbancy-concentration curve is shown in Fig. 1.And find concentration C mg/mL by absorbancy according to typical curve, then in extract, content and extraction yield have following formula to calculate:
Extractive content=[(C × 25 × 100)/(0.200 × 1000)] × 100%
Extraction yield=[(crude extract total mass × extractive content)/Ginkgo Leaf weight] × 100%
Extraction yield is 2.80%, and extractive content is 9.50%.
Embodiment 2 Folium Ginkgo extract and extracting method thereof
By Ginkgo Leaf impurity elimination, dry to constant weight for 60 DEG C in baking oven, pulverize, sieve with 60 mesh standard sieves.Get Ginkgo Leaf 10.000g, add the buffered soln 60mL that pH is respectively 4.8, adding 0.510g cellulase, to make it enzyme concn be 8.5g/L, in water-bath 50 DEG C after enzymolysis 1h, adding 140mL dehydrated alcohol makes system be 70% ethanolic soln, power 80W in micro computer microwave reactor, microwave temperature 70 DEG C, the accumulative microwave time is respectively 2min.
Suction filtration, filtrate removes chlorophyll and lipid 2 times with petroleum ether extraction, and water intaking layer rotary evaporation concentrates, and dries, obtain product in baking oven.
With the extraction yield of Folium Ginkgo total flavones in ultraviolet-spectrophotometer measurement product, measuring method is with embodiment 1, and recording extraction yield is 2.53%, and extractive content is 9.32%.
Embodiment 3 Folium Ginkgo extract and extracting method thereof
By Ginkgo Leaf impurity elimination, dry to constant weight for 60 DEG C in baking oven, pulverize, sieve with 60 mesh standard sieves.Take Ginkgo Leaf 10.000g, add the buffered soln 60mL that pH is respectively 4.8, adding 0.510g cellulase, to make it enzyme concn be 8.5g/L, in water-bath 50 DEG C after enzymolysis 1h, adding 140mL dehydrated alcohol makes system be 70% ethanolic soln, microwave power 130W in micro computer microwave reactor, microwave temperature 78 DEG C, the accumulative microwave time is respectively 4min.
Suction filtration, filtrate removes chlorophyll and lipid 2 times with petroleum ether extraction, and water intaking layer rotary evaporation concentrates, and dries, obtain product in baking oven.
With the extraction yield of Folium Ginkgo total flavones in ultraviolet-spectrophotometer measurement product, measuring method is with embodiment 1, and recording extraction yield is 3.96%, and extractive content is 10.34%.
Embodiment 4 Folium Ginkgo extract and extracting method thereof
By Ginkgo Leaf impurity elimination, dry to constant weight for 60 DEG C in baking oven, pulverize, sieve with 60 mesh standard sieves.Take Ginkgo Leaf 10.000g, add the sodium acetate-acetic acid buffered soln 60mL that pH is 4.8, add 0.454g cellulase to make it enzyme concn and be respectively 7.56g/L in water-bath 50 DEG C after enzymolysis 2h, adding 140mL dehydrated alcohol makes system be 70% ethanolic soln, microwave power 150W in micro computer microwave reactor, microwave temperature 80 DEG C, the accumulative microwave time is 4min.
Suction filtration, filtrate removes chlorophyll and lipid 2 times with petroleum ether extraction, and water intaking layer rotary evaporation concentrates, and dries, obtain product in baking oven.
With the extraction yield of Folium Ginkgo total flavones in ultraviolet-spectrophotometer measurement product, measuring method is with embodiment 1, and extraction yield is 3.46%, and extractive content is 9.76%.
Embodiment 5 Folium Ginkgo extract and extracting method thereof
By Ginkgo Leaf impurity elimination, dry to constant weight for 60 DEG C in baking oven, pulverize, sieve with 60 mesh standard sieves.Take Ginkgo Leaf 10.000g, add the sodium acetate-acetic acid buffered soln 60mL that pH is 4.8, adding 0.510g cellulase, to make it enzyme concn be 8.5g/L, after enzymolysis 1h in water-bath 60 DEG C, adding 140mL dehydrated alcohol makes system be 70% ethanolic soln, microwave power 150W in micro computer microwave reactor, microwave temperature 65 DEG C, the accumulative microwave time is 4min.
Suction filtration, filtrate removes chlorophyll and lipid 2 times with petroleum ether extraction, and water intaking layer rotary evaporation concentrates, and dries, obtain product in baking oven.
With the extraction yield of Folium Ginkgo total flavones in ultraviolet-spectrophotometer measurement product, measuring method is with embodiment 1, and extraction yield is 4.00%, and extractive content is 8.14%.
Embodiment 6 Folium Ginkgo extract and extracting method thereof
By Ginkgo Leaf impurity elimination, dry to constant weight for 60 DEG C in baking oven, pulverize, sieve with 60 mesh standard sieves.Take Ginkgo Leaf 10.000g, add the sodium acetate-acetic acid buffered soln 60mL that pH is 4.5, add 0.454g cellulase to make it enzyme concn and be respectively 7.56g/L in water-bath 50 DEG C after enzymolysis 2h, adding 140mL dehydrated alcohol makes system be 70% ethanolic soln, microwave power 300W in micro computer microwave reactor, microwave temperature 80 DEG C, the accumulative microwave time is 4min.
Suction filtration, filtrate removes chlorophyll and lipid 2 times with petroleum ether extraction, and water intaking layer rotary evaporation concentrates, and dries, obtain product in baking oven.
With the extraction yield of Folium Ginkgo total flavones in ultraviolet-spectrophotometer measurement product, measuring method is with embodiment 1, and extraction yield is 3.12%, and extractive content is 8.92%.
Embodiment 7 Folium Ginkgo extract and extracting method thereof
By Ginkgo Leaf impurity elimination, dry to constant weight for 60 DEG C in baking oven, pulverize, sieve with 60 mesh standard sieves.Take Ginkgo Leaf 10.000g, add the sodium acetate-acetic acid buffered soln 60mL that pH is 4.8, add 0.454g cellulase to make it enzyme concn and be respectively 7.56g/L in water-bath 50 DEG C after enzymolysis 2h, adding 60mL dehydrated alcohol makes system be 50% ethanolic soln, microwave power 150W in micro computer microwave reactor, microwave temperature 80 DEG C, the accumulative microwave time is 4min.
Suction filtration, filtrate removes chlorophyll and lipid 2 times with petroleum ether extraction, and water intaking layer rotary evaporation concentrates, and dries, obtain product in baking oven.
By the extraction yield of Folium Ginkgo total flavones in ultraviolet-spectrophotometer measurement product, measuring method embodiment 1, extraction yield is 3.00%, and extractive content is 7.46%.
Embodiment 8 Folium Ginkgo extract and extracting method thereof
By Ginkgo Leaf impurity elimination, dry to constant weight for 60 DEG C in baking oven, pulverize, sieve with 60 mesh standard sieves.Take Ginkgo Leaf 10.000g, add the sodium acetate-acetic acid buffered soln 60mL that pH is 4.8, add 0.454g cellulase to make it enzyme concn and be respectively 7.56g/L in water-bath 50 DEG C after enzymolysis 2h, adding 240mL dehydrated alcohol makes system be 80% ethanolic soln, microwave power 150W in micro computer microwave reactor, microwave temperature 80 DEG C, the accumulative microwave time is 4min.
Suction filtration, filtrate removes chlorophyll and lipid 2 times with petroleum ether extraction, and water intaking layer rotary evaporation concentrates, and dries, obtain product in baking oven.
With the extraction yield of Folium Ginkgo total flavones in ultraviolet-spectrophotometer measurement product, measuring method is with embodiment 1, and extraction yield is 3.80%, and extractive content is 8.96%.
Comparative example 1
Get Ginkgo Leaf 10.000g, add 140mL dehydrated alcohol and make system be 70% ethanolic soln, power 80W in micro computer microwave reactor, microwave temperature 70 DEG C, it is 1.50% that the accumulative microwave time is respectively 2min extraction yield, and extractive content is 4.52%.
Comparative example 2
Take Ginkgo Leaf 10.000g, add the buffered soln 60mL that pH is respectively 4.8, adding 0.510g cellulase, to make it enzyme concn be 8.50g/L, in water-bath 50 DEG C after enzymolysis 1h, add 140mL dehydrated alcohol and make system be 70% ethanolic soln, microwave power 130W in micro computer microwave reactor, microwave temperature 38 DEG C, the accumulative microwave time is respectively 6min, and extraction yield is 1.40%, and extractive content is 6.32%.
Comparative example 3
By Ginkgo Leaf impurity elimination, dry to constant weight for 60 DEG C in baking oven, pulverize, sieve with 60 mesh standard sieves.Take Ginkgo Leaf 10.000g, add the sodium acetate-acetic acid buffered soln 60mL that pH is 4.8, add 0.454g cellulase to make it enzyme concn and be respectively 7.56g/L in water-bath 50 DEG C after enzymolysis 2h, adding 140mL dehydrated alcohol makes system be 70% ethanolic soln, microwave power 150W in micro computer microwave reactor, microwave temperature 80 DEG C, the accumulative microwave time is 15min, suction filtration, filtrate removes green element and lipid 2 times with petroleum ether extraction, water intaking layer rotary evaporation concentrates, dry in baking oven, claim crude extract quality, measure absorbancy, extraction yield is 2.05%, extractive content is 5.43%.
Comparative example 4
By Ginkgo Leaf impurity elimination, dry to constant weight for 60 DEG C in baking oven, pulverize, sieve with 60 mesh standard sieves.Take Ginkgo Leaf 10.000g, add the sodium acetate-acetic acid buffered soln 60mL that pH is 4.8, adding 0.510g cellulase, to make it enzyme concn be 8.50g/L, after enzymolysis 1h in water-bath 60 DEG C, adding 140mL dehydrated alcohol makes system be 70% ethanolic soln, microwave power 30W in micro computer microwave reactor, microwave temperature 65 DEG C, the accumulative microwave time is 4min, suction filtration, filtrate removes chlorophyll and lipid 2 times with petroleum ether extraction, water intaking layer rotary evaporation concentrates, dry in baking oven, claim crude extract quality, measure absorbancy, extraction yield is 1.40%, extractive content is 3.14%.
The articles for washing of embodiment 9 containing Folium Ginkgo extract
Folium Ginkgo extract 0.4g described in embodiment 1, vitamins C 0.4g, sapindoside 100g, sandalwood essential oil 0.2g, water purification 1Kg, heat 45 DEG C and stir, cool.
Soil removability test is see GB/T13174 ~ 2008, and test result is: decontamination value R85.8, can be dissolved in cold water arbitrarily.
The articles for washing of embodiment 10 containing Folium Ginkgo extract
Sodium hydroxide 80g, pure water 300g are heated with stirring to 50 DEG C of dissolvings, add Gleditisia sinensis Saponin 100g, Folium Ginkgo extract 0.8g described in embodiment 1, sweet oil 100g, stir 60min, and powder made by spray-dried and cyclonic separator.
Soil removability test is see GB/T13174 ~ 2008, and test result is: decontamination value R73.8,10g/100g hydroecium temperature solubilized.
The articles for washing of embodiment 11 containing Folium Ginkgo extract
Folium Ginkgo extract 0.3g described in embodiment 1, tea saponin 40g, sapindoside 100g, propylene glycol 5g, glycerine hydrolytic soya bean protein 1g, Radix Ginseng extractive solution 0.2g, hydrolytic soya bean protein 10g essence 0.1g, methyl hydroxybenzoate 5g, pure water 3kg, 55 DEG C stir, cool.
Soil removability test is see GB/T13174 ~ 2008, and test result is: decontamination value R75.6,10g/50g hydroecium temperature solubilized.
Comparative example 5
Vitamins C 0.4g, sapindoside 50 ~ 100g, sandalwood essential oil 0.2g, water purification 1Kg, heat 45 DEG C and stir, cool.Soil removability test is see GB/T13174 ~ 2008, and test result is: decontamination value 69.0,30g/50g50 DEG C of warm water solubilized.
Comparative example 6
Tea saponin 40g, sapindoside 100g, propylene glycol 5g, glycerine are hydrolyzed mostly albumen 1g, Radix Ginseng extractive solution 0.2g, hydrolytic soya bean protein 10g essence 0.1g, methyl hydroxybenzoate 5g, pure water 3kg, and 55 DEG C stir, cool.Soil removability test is see GB/T13174 ~ 2008, and test result is: decontamination value R70.9,10g/50g water 45 DEG C of solubilized.
Although above the present invention is described in detail with a general description of the specific embodiments, on basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, these modifications or improvements without departing from theon the basis of the spirit of the present invention, all belong to the scope of protection of present invention.
Claims (10)
1. a Folium Ginkgo extract, is characterized in that, its extracting method comprises the steps:
1) Ginkgo Leaf is dried, pulverize;
2) get Ginkgo Leaf, add the sodium acetate-acetic acid buffered soln of pH4.0 ~ 5.5, add cellulase, enzymolysis 1 ~ 2h in the water-bath of 50 ~ 60 DEG C;
3) add dehydrated alcohol, in microwave reactor, carry out microwave extraction, control microwave power is 50 ~ 300W, microwave temperature 50 ~ 80 DEG C, and the accumulative microwave time is 1 ~ 8min;
4) suction filtration, filtrate removes impurity with petroleum ether extraction, and water intaking layer rotary evaporation concentrates, and dries, to obtain final product.
2. Folium Ginkgo extract according to claim 1, is characterized in that, described step 2) get Ginkgo Leaf, add the sodium acetate-acetic acid buffered soln of pH4.8, add cellulase, enzymolysis 1h in the water-bath of 50 DEG C.
3. Folium Ginkgo extract according to claim 1 and 2, is characterized in that, described step 3) add dehydrated alcohol, in microwave reactor, carry out microwave extraction, control microwave power is 130W, microwave temperature 78 DEG C, and the accumulative microwave time is 4min.
4. Folium Ginkgo extract according to claim 3, is characterized in that, after adding dehydrated alcohol, in system, the volume fraction of ethanol is 50 ~ 80%.
5. Folium Ginkgo extract according to claim 4, is characterized in that, after adding dehydrated alcohol, the solid-liquid ratio of Ginkgo Leaf and solution system is 1:5 ~ 1:50.
6. an extracting method for Folium Ginkgo extract, is characterized in that, described method comprises the steps:
1) Ginkgo Leaf is dried, pulverize;
2) get Ginkgo Leaf, add the sodium acetate-acetic acid buffered soln of pH4.0 ~ 5.5, add cellulase, enzymolysis 1 ~ 2h in the water-bath of 50 ~ 60 DEG C;
3) add dehydrated alcohol, in microwave reactor, carry out microwave extraction, control microwave power is 50 ~ 300W, microwave temperature 50 ~ 80 DEG C, and the accumulative microwave time is 1 ~ 8min;
4) suction filtration, filtrate removes impurity with petroleum ether extraction, and water intaking layer rotary evaporation concentrates, and dries, to obtain final product.
7. method according to claim 6, is characterized in that, described step 2) get Ginkgo Leaf, add the sodium acetate-acetic acid buffered soln of pH4.8, add cellulase, enzymolysis 1h in the water-bath of 50 DEG C;
Described step 3) add dehydrated alcohol, the volume fraction making ethanol in system is 50 ~ 80%, in microwave reactor, carry out microwave extraction, and control microwave power is 130W, microwave temperature 78 DEG C, and the accumulative microwave time is 4min.
8. the cleaning product containing Folium Ginkgo extract described in any one of Claims 1 to 5.
9. cleaning product according to claim 8, is characterized in that, described cleaning product contains saponin(e and/or saponin.
10. the application of the Folium Ginkgo extract described in any one of Claims 1 to 5 in preparing washing product.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107751557A (en) * | 2016-08-22 | 2018-03-06 | 北京大伟嘉生物技术股份有限公司 | A kind of Chinese herb feed additive for reducing egg cholesterol and its application |
| CN106520858A (en) * | 2016-10-20 | 2017-03-22 | 徐州工程学院 | Extraction method for water-soluble dietary fibre of ginkgo leaf |
| CN107019712A (en) * | 2017-05-19 | 2017-08-08 | 临沂大学 | A kind of extraction process of ginkgo leaf |
| CN114907896A (en) * | 2022-04-27 | 2022-08-16 | 南京林业大学 | Ginkgo leaf enzymolysis treatment method and application thereof |
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