CN111466479A - Comprehensive preparation method of safflower seed multi-product - Google Patents
Comprehensive preparation method of safflower seed multi-product Download PDFInfo
- Publication number
- CN111466479A CN111466479A CN201911341706.3A CN201911341706A CN111466479A CN 111466479 A CN111466479 A CN 111466479A CN 201911341706 A CN201911341706 A CN 201911341706A CN 111466479 A CN111466479 A CN 111466479A
- Authority
- CN
- China
- Prior art keywords
- safflower seed
- safflower
- protein
- column
- washing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 244000020518 Carthamus tinctorius Species 0.000 title claims abstract description 229
- 235000003255 Carthamus tinctorius Nutrition 0.000 title claims abstract description 228
- 238000002360 preparation method Methods 0.000 title claims abstract description 38
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 77
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 77
- 239000003813 safflower oil Substances 0.000 claims abstract description 64
- 239000000843 powder Substances 0.000 claims abstract description 44
- QZAYGJVTTNCVMB-UHFFFAOYSA-N serotonin Chemical class C1=C(O)C=C2C(CCN)=CNC2=C1 QZAYGJVTTNCVMB-UHFFFAOYSA-N 0.000 claims abstract description 27
- 238000000194 supercritical-fluid extraction Methods 0.000 claims abstract description 17
- 238000005406 washing Methods 0.000 claims description 38
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims description 36
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 33
- 238000009835 boiling Methods 0.000 claims description 33
- 239000004744 fabric Substances 0.000 claims description 28
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 27
- 239000000047 product Substances 0.000 claims description 27
- 238000000605 extraction Methods 0.000 claims description 25
- 239000000945 filler Substances 0.000 claims description 22
- 238000000034 method Methods 0.000 claims description 22
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims description 20
- 238000001035 drying Methods 0.000 claims description 20
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 claims description 18
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 claims description 18
- 239000011259 mixed solution Substances 0.000 claims description 18
- 238000002156 mixing Methods 0.000 claims description 18
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 17
- 238000001914 filtration Methods 0.000 claims description 17
- 239000000243 solution Substances 0.000 claims description 16
- 238000003756 stirring Methods 0.000 claims description 16
- 238000009777 vacuum freeze-drying Methods 0.000 claims description 15
- 230000008569 process Effects 0.000 claims description 14
- 239000001569 carbon dioxide Substances 0.000 claims description 10
- 229910002092 carbon dioxide Inorganic materials 0.000 claims description 10
- 239000000706 filtrate Substances 0.000 claims description 10
- 239000000084 colloidal system Substances 0.000 claims description 9
- 238000007710 freezing Methods 0.000 claims description 9
- 230000008014 freezing Effects 0.000 claims description 9
- 239000002244 precipitate Substances 0.000 claims description 9
- 238000010298 pulverizing process Methods 0.000 claims description 9
- 229910000030 sodium bicarbonate Inorganic materials 0.000 claims description 9
- 235000017557 sodium bicarbonate Nutrition 0.000 claims description 9
- 229910000029 sodium carbonate Inorganic materials 0.000 claims description 9
- 241000233866 Fungi Species 0.000 claims description 7
- 239000003995 emulsifying agent Substances 0.000 claims description 7
- 239000003337 fertilizer Substances 0.000 claims description 7
- 238000000227 grinding Methods 0.000 claims description 7
- 230000006872 improvement Effects 0.000 claims description 7
- 239000006210 lotion Substances 0.000 claims description 7
- 239000002689 soil Substances 0.000 claims description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 7
- 238000004519 manufacturing process Methods 0.000 claims description 6
- 102000006947 Histones Human genes 0.000 claims description 3
- 108010033040 Histones Proteins 0.000 claims description 3
- 238000005516 engineering process Methods 0.000 claims description 2
- 239000010903 husk Substances 0.000 claims 1
- 238000012856 packing Methods 0.000 claims 1
- 235000012970 cakes Nutrition 0.000 description 39
- 239000003921 oil Substances 0.000 description 24
- 235000019198 oils Nutrition 0.000 description 24
- 239000000203 mixture Substances 0.000 description 22
- 239000007788 liquid Substances 0.000 description 17
- 235000009508 confectionery Nutrition 0.000 description 16
- 239000003826 tablet Substances 0.000 description 12
- 238000011049 filling Methods 0.000 description 11
- 235000013305 food Nutrition 0.000 description 11
- 239000000725 suspension Substances 0.000 description 11
- 239000000463 material Substances 0.000 description 10
- 235000012054 meals Nutrition 0.000 description 10
- 239000008187 granular material Substances 0.000 description 9
- 239000006187 pill Substances 0.000 description 9
- 235000013361 beverage Nutrition 0.000 description 8
- 239000006188 syrup Substances 0.000 description 8
- 235000020357 syrup Nutrition 0.000 description 8
- 230000036541 health Effects 0.000 description 7
- 235000015895 biscuits Nutrition 0.000 description 6
- 239000002775 capsule Substances 0.000 description 6
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 6
- 239000002994 raw material Substances 0.000 description 6
- 108010082495 Dietary Plant Proteins Proteins 0.000 description 5
- 241001649158 Oroxylum Species 0.000 description 5
- 235000012920 Oroxylum indicum Nutrition 0.000 description 5
- 238000007605 air drying Methods 0.000 description 5
- 239000003814 drug Substances 0.000 description 5
- 239000008157 edible vegetable oil Substances 0.000 description 5
- 239000000284 extract Substances 0.000 description 5
- 238000004806 packaging method and process Methods 0.000 description 5
- 239000002245 particle Substances 0.000 description 5
- 235000021568 protein beverage Nutrition 0.000 description 5
- 210000000582 semen Anatomy 0.000 description 5
- 229940079593 drug Drugs 0.000 description 4
- OYHQOLUKZRVURQ-HZJYTTRNSA-N Linoleic acid Chemical compound CCCCC\C=C/C\C=C/CCCCCCCC(O)=O OYHQOLUKZRVURQ-HZJYTTRNSA-N 0.000 description 3
- 235000005911 diet Nutrition 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 235000013402 health food Nutrition 0.000 description 3
- OYHQOLUKZRVURQ-IXWMQOLASA-N linoleic acid Natural products CCCCC\C=C/C\C=C\CCCCCCCC(O)=O OYHQOLUKZRVURQ-IXWMQOLASA-N 0.000 description 3
- 235000020778 linoleic acid Nutrition 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 239000006227 byproduct Substances 0.000 description 2
- 235000012000 cholesterol Nutrition 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000000378 dietary effect Effects 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 231100000331 toxic Toxicity 0.000 description 2
- 230000002588 toxic effect Effects 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 201000001320 Atherosclerosis Diseases 0.000 description 1
- 208000037260 Atherosclerotic Plaque Diseases 0.000 description 1
- 231100000699 Bacterial toxin Toxicity 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000628997 Flos Species 0.000 description 1
- 208000031226 Hyperlipidaemia Diseases 0.000 description 1
- 235000019485 Safflower oil Nutrition 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 208000011775 arteriosclerosis disease Diseases 0.000 description 1
- 239000000688 bacterial toxin Substances 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 208000029078 coronary artery disease Diseases 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 235000015872 dietary supplement Nutrition 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- -1 ferulomine Chemical compound 0.000 description 1
- 238000010262 high-speed countercurrent chromatography Methods 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229930014626 natural product Natural products 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 231100000956 nontoxicity Toxicity 0.000 description 1
- 230000009965 odorless effect Effects 0.000 description 1
- 239000003895 organic fertilizer Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 235000005713 safflower oil Nutrition 0.000 description 1
- 235000014214 soft drink Nutrition 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/006—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from vegetable materials
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/185—Vegetable proteins
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D209/00—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom
- C07D209/02—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom condensed with one carbocyclic ring
- C07D209/04—Indoles; Hydrogenated indoles
- C07D209/10—Indoles; Hydrogenated indoles with substituted hydrocarbon radicals attached to carbon atoms of the hetero ring
- C07D209/14—Radicals substituted by nitrogen atoms, not forming part of a nitro radical
- C07D209/16—Tryptamines
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K17/00—Soil-conditioning materials or soil-stabilising materials
- C09K17/40—Soil-conditioning materials or soil-stabilising materials containing mixtures of inorganic and organic compounds
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/02—Pretreatment
- C11B1/04—Pretreatment of vegetable raw material
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/10—Production of fats or fatty oils from raw materials by extracting
- C11B1/104—Production of fats or fatty oils from raw materials by extracting using super critical gases or vapours
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B3/00—Refining fats or fatty oils
- C11B3/001—Refining fats or fatty oils by a combination of two or more of the means hereafter
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B3/00—Refining fats or fatty oils
- C11B3/16—Refining fats or fatty oils by mechanical means
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K2101/00—Agricultural use
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/54—Improvements relating to the production of bulk chemicals using solvents, e.g. supercritical solvents or ionic liquids
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Polymers & Plastics (AREA)
- Inorganic Chemistry (AREA)
- Mycology (AREA)
- Food Science & Technology (AREA)
- Biochemistry (AREA)
- Environmental Sciences (AREA)
- General Life Sciences & Earth Sciences (AREA)
- Soil Sciences (AREA)
- Materials Engineering (AREA)
- Mechanical Engineering (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Medicines Containing Plant Substances (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses a comprehensive preparation method of safflower seed multi-product, which comprises the following steps: the preparation method comprises the steps of separating and preparing safflower seed shell powder from safflower seed shells at high efficiency and low cost, performing supercritical extraction on safflower seed kernels to prepare safflower seed oil, separating and purifying safflower seed protein to prepare safflower seed protein isolate powder, and extracting and preparing safflower seed 5-hydroxytryptamine derivatives. Through the steps, the comprehensive preparation of 4 products of safflower seed shell powder, safflower seed oil, safflower seed protein isolate and safflower seed 5-hydroxytryptamine derivatives is realized, the resources are saved, and the added value of the products is improved.
Description
Technical Field
The invention relates to the technical field of safflower seed processing, in particular to a comprehensive preparation method of safflower seed multi-product.
Background
Safflower seed, named as "white flatseed" as a traditional Chinese medicine, is the seed of Safflower Safflower (Carthamustincorusius L.) Safflower seed meal is a byproduct of Safflower oil extracted from Safflower seed, Safflower is an ancient 1-2 year old herbaceous plant, has a history of 4000 years in ancient Egyptian, and has 20-25 varieties of flowers recognized by Zhang Qianlia, and introduces Safflower into the existing 2100 years of cultivation and medication history in China, at present, Safflower is mainly distributed in provinces (districts) such as Xinjiang, Qinghai, Gansu, and the like, most flowers of Safflower are used as medicines, Safflower seed meal, Safflower seed oil and Safflower seed shells are rarely utilized, enough attention is not drawn, many resources are wasted, the defatted part of Safflower seed meal is used as a feed or an organic fertilizer for most livestock in many countries and regions, deoiled Safflower seed meal contains abundant proteins, has no toxicity, no peculiar smell, 18 amino acids, is a protein which is superior to protein, 1966, the protein meal is used as a concentrated antioxidant protein powder for preventing the death of Safflower seed meal, and the protein is used for the preparation of a food for resisting bacterial-toxin, the protein suspension, the protein and the protein suspension for people, the protein suspension, the protein for resisting human body, the protein suspension for resisting and the protein for resisting and the protein suspension, the protein for resisting human body, the protein suspension for resisting the protein suspension, the protein suspension for resisting human body, the protein suspension for resisting the protein suspension, the protein suspension for.
The oil-containing part of flower and seed of Carthami flos has effects of softening blood vessel, reducing blood lipid and cholesterol, and preventing atherosclerosis. The content of linoleic acid in the safflower seed oil is up to 78-85 percent, the linoleic acid is the only fatty acid which is necessary for human bodies and cannot be synthesized in the human bodies, and the linoleic acid is necessary to be supplied by diet, can reduce the cholesterol level, and reduce the chance of cholesterol precipitation on atherosclerotic plaques, thereby effectively preventing hyperlipidemia, arteriosclerosis and coronary heart disease, and having high medicinal value.
The traditional preparation process of safflower seed oil is a squeezing method, so that more than 10% of oil is left in cake, not only is waste caused, but also how to utilize the cake is always a concern of people. The deoiled safflower seed meal contains rich protein, and is non-toxic and odorless. However, the medicinal and nutritional values of safflower seed meal are mainly determined by the degree of dehulling, and safflower seed meal with complete dehulling has high medicinal and nutritional values. Through search, research on development and preparation of safflower seeds is more, but most of the research is limited to preparation of single components, the cake obtained after traditional oil extraction of safflower seeds contains about 19% of histones, is very difficult to utilize, and when the protein is extracted from the cake, the cost is high, the process is complex, the yield is low, and a plurality of byproducts are difficult to utilize, so that great difficulty is brought to industrialization of safflower seeds. For example, a method for producing safflower seed protein powder disclosed in Chinese patent application No. 201811427122.3, a method for extracting safflower seed oil by supercritical carbon dioxide disclosed in Chinese patent application No. 200610048200.X, and a method for preparing 5-hydroxytryptamine derivatives from safflower seed meal by high-speed counter-current chromatography disclosed in Chinese patent application No. 201310402221.7.
The above methods have limitations in more or less single development, resource waste, use of harmful reagents, high cost, difficulty in industrialization, low yield, etc., which limit their applications. In the current report, no research is reported for preparing four effective components or tissue parts (comprising safflower seed shell powder, safflower seed oil, safflower seed isolate protein and safflower seed 5-hydroxytryptamine derivatives) of safflower seeds with different attributes at one time by adopting a comprehensive preparation method.
Disclosure of Invention
The invention aims to solve the defects in the prior art and provide a comprehensive preparation method of safflower seed multi-product, which can be directly used for industrially preparing safflower seed shell powder, safflower seed oil, safflower seed isolate protein and safflower seed 5-hydroxytryptamine derivatives.
In order to achieve the purpose, the invention is implemented according to the following technical scheme:
the first technical scheme of the invention is as follows: a comprehensive preparation method of safflower seed multi-product comprises the following steps:
s1, washing safflower seeds with water, drying in a hot air circulation drying oven, shelling with a safflower seed sheller to obtain safflower seed shells and safflower seed kernels, pulverizing the safflower seed shells into safflower seed shell powder with an ultrafine pulverizer, and bagging for later use;
s2, grinding the safflower seeds prepared in the step S1 by using a colloid mill, placing the ground safflower seeds into supercritical extraction equipment, and extracting crude safflower seed oil by using a carbon dioxide supercritical extraction technology; freezing the crude safflower seed oil, centrifuging, filtering to obtain refined safflower seed oil, safflower seed cake and safflower seed oil residue, and bottling;
s3, boiling and washing the safflower seed cake prepared in the step S2 with a mixed solution of ethanol and ethyl acetate, centrifuging and filtering while the solution is hot after boiling and washing to obtain a safflower seed protein boiling and washing solution and safflower seed tissue protein;
s4, extracting the safflower seed histones prepared in the step S3 by using a mixed solution of sodium carbonate and sodium bicarbonate; centrifuging and filtering after extraction, adjusting pH of the filtrate with acetic acid, standing, centrifuging and filtering, vacuum freeze drying the obtained precipitate to obtain safflower seed protein isolate, pulverizing, and bagging;
s5, placing the safflower seed protein boiling lotion prepared in the step S3 and the safflower seed oil filter residue prepared in the step S2 into a homogenizing emulsifier for homogenizing and stirring, carrying out column passing on the mixed solution, concentrating the filtrate, carrying out vacuum freeze drying to prepare a safflower seed 5-hydroxytryptamine derivative, and bagging for later use;
and S6, collecting the residues of the column fillers after the column is passed through in the step S5, airing the collected residues in an open field for standby, and using the residues for cultivation of edible fungi, preparation of organic fertilizers and soil improvement.
Further, in the step S1, the drying temperature of the safflower seeds in the hot air circulation drying oven is 30-60 ℃, the drying time is 2-6 hours, and the granularity of the safflower seed shell powder is 100-300 meshes.
Further, in the step S2, the safflower seeds are ground by a colloid mill to have the granularity of 0.1-0.6mm, the supercritical extraction temperature of carbon dioxide is 20-60 ℃, the extraction pressure is 10-50 MPa, and the extractant CO is2The flow rate is 5-40L/h, the extraction time is 60-300 min, the freezing temperature of the crude safflower seed oil is-20-0 ℃, and the crude safflower seed oil is frozen and then is centrifugally filtered by filter cloth of 100-300 meshes.
Further, the mixed solution of ethanol and ethyl acetate in the step S3 is prepared by mixing ethanol and ethyl acetate according to the volume ratio of 1: 3-3: 1, the mass ratio of the mixed solution of ethanol and ethyl acetate to the safflower seed cake is 1.0-2.0, the boiling and washing temperature is 40-70 ℃, the boiling and washing time is 30-150 min, and the hot mixture is subjected to centrifugal filtration by using 100-300-mesh filter cloth after the boiling and washing.
Further, in the step S4, the pH value of the mixed solution of sodium carbonate and sodium bicarbonate is 8-10, the extraction time is 30-120 min, the extraction temperature is 30-60 ℃, and the stirring is continuously carried out during the extraction; and after extraction, carrying out centrifugal filtration by using 100-300-mesh filter cloth, adding acetic acid into filtrate to adjust the pH value to 2.0-4.0, standing for 12-24 h below 20 ℃, carrying out centrifugal filtration by using 100-300-mesh filter cloth, carrying out vacuum freeze drying on the obtained precipitate at the vacuum degree of 30-100 Pa and the temperature of-30-15 ℃ for 12-24 h to obtain safflower seed protein isolate, and crushing the safflower seed protein isolate into 60-100 meshes.
Further, the homogenizing and stirring time of the step S5 is 0.5-2 h, the filler of the column is mixture of safflower seed shell powder and diatomite according to the mass ratio of 1: 3-3: 1, the inner diameter of the column is 100mm, the height of the column is 1000mm, and the flow rate is 60-120 m L/min.
The second technical scheme of the invention is as follows: a safflower seed hull powder is prepared by the above method.
The third technical scheme of the invention is as follows: a safflower seed oil is prepared by the above method.
The fourth technical scheme of the invention is as follows: a safflower seed protein isolate is prepared by the above method.
The fifth technical scheme of the invention is as follows: a safflower seed 5-hydroxytryptamine derivative is prepared by the above method.
Compared with the prior art, the invention has the following beneficial effects:
(1) the invention provides a comprehensive preparation method of safflower seed multi-product, mainly obtains four finished products of safflower seed shell powder, safflower seed oil, safflower seed protein isolate and safflower seed 5-hydroxytryptamine derivative, can be used in medicines, health products or foods with treatment and health care effects, and has high comprehensive utilization rate, low comprehensive cost of each product and high added value of the product;
(2) the comprehensive preparation method of the invention does not relate to toxic and harmful preparations, and the products are all from the effective parts of natural products, have no toxic or side effect and high safety;
(3) the raw material resources of the invention are easy to obtain, the fixed asset investment of the production line is not large, the industrialization is easy, and the investment return period is short;
(4) the application of the product preparation in preparing medicines, health-care foods or foods with treatment and health-care functions can effectively meet the national standard, and no market barrier exists.
Drawings
FIG. 1 is a flow chart of the comprehensive preparation method of safflower seed multi-product of the present invention.
FIG. 2 is a chemical structural diagram of a safflower seed 5-hydroxytryptamine derivative of the present invention.
FIG. 3 is a high performance liquid chromatogram of a safflower seed 5-hydroxytryptamine derivative according to the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. The specific embodiments described herein are merely illustrative of the invention and do not limit the invention.
Example 1
Referring to FIG. 1, the comprehensive preparation method of safflower seed multi-product of this example comprises the following steps:
(1) taking 100kg of safflower seed raw material, washing for 10min by a high-pressure water gun, drying for 2 hours at 30 ℃ by a hot air circulation drying box, carrying out hulling treatment by a special huller to obtain about 25kg of safflower seed shells and about 65kg of safflower seed kernels, crushing the safflower seed shells into 100 meshes by an ultrafine pulverizer to obtain 20kg of safflower seed shell ultrafine powder, and filling 5kg of safflower seed shells into each bag for later use;
(2) grinding 65kg of semen Carthami with colloid mill to particle size of 0.1mm, placing in supercritical extraction equipment, extracting with carbon dioxide supercritical extraction at 20 deg.C under 10MPa with CO as extractant2The flow rate is 5L/h, the extraction time is 60min, 25kg of crude safflower seed oil is obtained, after freezing at the temperature of minus 20 ℃, the crude safflower seed oil is centrifugally filtered by a filter cloth with 100 meshes, 23kg of refined safflower seed oil and 37.5kg of safflower seed cake and 1.5kg of safflower seed oil filter residue are obtained, 500ml of refined safflower seed oil is filled in each bottle, and the safflower seed cake and the safflower seed oil filter residue are reserved, and the refined safflower seed oil in the embodiment is directly used as edible oil for daily use.
(3) The safflower seed cake dregs of 37.5kg prepared in the above steps are boiled and washed by ethanol/ethyl acetate according to the mixing ratio of 1:3, the mass ratio of the boiled and washed liquid to the cake dregs is 1.0 and 75kg in total, the boiling and washing temperature is 40 ℃, the boiling and washing time is 30min, and the safflower seed protein boiled and washed liquid is centrifugally filtered by a filter cloth of 100 meshes while the safflower seed protein boiled and washed liquid is hot, so that 65kg of safflower seed protein boiled and washed liquid and 42kg of safflower seed tissue protein are obtained;
(4) extracting 42kg of safflower seed tissue protein with mixed solution of sodium carbonate and sodium bicarbonate at pH of 8 for 30min and extraction temperature of 30 deg.C under stirring; centrifuging with 100 mesh filter cloth, adjusting pH to 2.0 with acetic acid, standing at 20 deg.C or below for 12 hr, centrifuging with 100 mesh filter cloth, vacuum freeze drying the precipitate at-30 deg.C under vacuum degree of 30Pa for 12 hr to obtain 30kg of safflower seed protein isolate, pulverizing to 60 mesh, and packaging into 5kg bags; the safflower seed separated egg of the embodiment can be added with conventional auxiliary materials and prepared into clinically or commercially acceptable tablets, capsules, powders, mixtures, pills, granules, solutions, syrups, soft extracts, beverages, biscuits, candies or cakes, puffed foods, vegetable protein beverages, blend oils, health care oils, massage oils, daily nuts, pressed candies, oroxylum films and the like according to a conventional process.
(5) Mixing 65kg of safflower seed protein boiled lotion prepared in the step (3) and 1.5kg of safflower seed oil filter residue prepared in the step (2), placing the mixture in a homogenizing emulsifier, homogenizing and stirring for 0.5h, passing the mixture through a column, mixing the safflower seed shell powder prepared in the step (1) and diatomite according to a ratio of 1:3 to obtain column filler, consuming 5kg of safflower seed shell powder and 5kg of diatomite together, wherein the inner diameter of the column is 100mm, the height of the column is 1000mm, the outlet flow rate of the column is kept at 60m L/min, carrying out vacuum concentration (vacuum degree-0.09 MPa and temperature of 30-40 ℃) on the filtrate for 12h, carrying out vacuum freeze drying (vacuum degree of 30-50 Pa and temperature of-30-25 ℃) to obtain 800g of safflower seed 5-hydroxytryptamine derivatives, and filling 100g of each bag for standby, wherein the safflower seed 5-hydroxytryptamine derivatives can be added with conventional auxiliary materials and prepared into clinically or commercially acceptable tablets, capsules, powders, pills, granules, solutions, decoctions, soft drinks, massage paste, health-food oil, biscuits, candy, puffed oil, candy and the like according to the conventional process.
(6) And (3) after the column is passed through in the step (5), collecting the filler residues (such as safflower seed shell powder, kieselguhr and the like) of the column intensively, and airing and air-drying the collected filler residues in open lands to obtain 9kg of filler residues, wherein the residues can be used for cultivation of high-end edible fungi, preparation of organic fertilizers, soil improvement and the like.
Example 2
Referring to FIG. 1, the comprehensive preparation method of safflower seed multi-product of this example comprises the following steps:
(1) taking 100kg of safflower seed raw material, washing for 10min by a high-pressure water gun, drying for 6 hours at 60 ℃ by a hot air circulation drying box, carrying out hulling treatment by a special huller to obtain about 25kg of safflower seed shells and about 65kg of safflower seed kernels, crushing the safflower seed shells into 300 meshes by an ultrafine pulverizer to obtain 20kg of safflower seed shell ultrafine powder, and filling 5kg of safflower seed shells into each bag for later use;
(2) grinding 65kg of semen Carthami with colloid mill to particle size of 0.6mm, placing in supercritical extraction equipment, extracting with carbon dioxide supercritical extraction at 60 deg.C under 50MPa with CO as extractant2The flow rate is 40L/h, the extraction time is 300min, 25kg of crude safflower seed oil is obtained, after freezing at 0 ℃, the crude safflower seed oil is centrifugally filtered by 300-mesh filter cloth, 23kg of refined safflower seed oil and 37.5kg of safflower seed cake and 1.5kg of safflower seed oil filter residue are obtained, the refined safflower seed oil is filled into 500ml bottles, and the safflower seed cake and the safflower seed filter residue are reserved, and the refined safflower seed oil in the embodiment is directly used as edible oil for daily use.
(3) The safflower seed cake dregs of 37.5kg prepared in the above steps are boiled and washed by ethanol/ethyl acetate according to the mixing ratio of 3:1, the mass ratio of the boiled and washed liquid to the cake dregs is 2.0 and 75kg in total, the boiling and washing temperature is 70 ℃, the boiling and washing time is 150min, and the safflower seed protein boiled and washed liquid is centrifugally filtered by a filter cloth of 300 meshes while the safflower seed protein boiled and washed liquid is hot, so that 65kg of safflower seed protein boiled and washed liquid and 42kg of safflower seed tissue protein are obtained;
(4) extracting 42kg of safflower seed tissue protein with mixed solution of sodium carbonate and sodium bicarbonate at pH of 10 for 120min and extraction temperature of 60 deg.C under stirring; centrifuging and filtering with 300 mesh filter cloth, adjusting pH to 4.0 with acetic acid, standing at 20 deg.C or below for 24 hr, centrifuging and filtering with 300 mesh filter cloth, vacuum freeze drying the obtained precipitate at-15 deg.C under 100Pa to obtain 30kg of safflower seed protein isolate, pulverizing to 100 mesh, and packaging into 5kg bags; the safflower seed separated egg of the embodiment can be added with conventional auxiliary materials and prepared into clinically or commercially acceptable tablets, capsules, powders, mixtures, pills, granules, solutions, syrups, soft extracts, beverages, biscuits, candies or cakes, puffed foods, vegetable protein beverages, blend oils, health care oils, massage oils, daily nuts, pressed candies, oroxylum films and the like according to a conventional process.
(5) Mixing 65kg of safflower seed protein boiled lotion prepared in the step (3) and 1.5kg of safflower seed oil filter residue prepared in the step (2), placing the mixture in a homogenizing emulsifier, homogenizing and stirring for 2h, passing the mixture through a column, mixing the safflower seed shell powder prepared in the step (1) and diatomite according to a ratio of 3:1 to obtain a column filler, consuming 5kg of safflower seed shell powder and 5kg of diatomite together, wherein the inner diameter of the column is 100mm, the height of the column is 1000mm, the outlet flow rate of the column is kept at 120m L/min, carrying out vacuum concentration (vacuum degree-0.09 MPa and temperature of 30-40 ℃) on the filtrate for 24h, carrying out vacuum freeze drying (vacuum degree of 30-50 Pa and temperature of-30-25 ℃) to obtain 800g of safflower seed 5-hydroxytryptamine derivatives, and filling 100g of each bag for standby, wherein the safflower seed 5-hydroxytryptamine derivatives can be added with conventional auxiliary materials and prepared into clinically or commercially acceptable tablets, pills, powder, tablets, granules, solutions, syrups, decoction, beverages, drinks, massage paste, health-food, cakes, health-care oil, cakes, candies, and the like according to conventional processes.
(6) And (3) after the column is passed through in the step (5), collecting the filler residues (such as safflower seed shell powder, kieselguhr and the like) of the column intensively, and airing and air-drying the collected filler residues in open lands to obtain 9kg of filler residues, wherein the residues can be used for cultivation of high-end edible fungi, preparation of organic fertilizers, soil improvement and the like.
Example 3
Referring to FIG. 1, the comprehensive preparation method of safflower seed multi-product of this example comprises the following steps:
(1) taking 100kg of safflower seed raw material, washing for 10min by a high-pressure water gun, drying for 4 hours at 40 ℃ by a hot air circulation drying box, carrying out hulling treatment by a special huller to obtain about 25kg of safflower seed shells and about 65kg of safflower seed kernels, crushing the safflower seed shells into 200 meshes by an ultrafine pulverizer to obtain 20kg of safflower seed shell ultrafine powder, and filling 5kg of safflower seed shells into each bag for later use;
(2) grinding 65kg of semen Carthami with colloid mill to particle size of 0.4mm, placing in supercritical extraction equipment, extracting with carbon dioxide supercritical extraction at 30 deg.C under 30MPa with CO as extractant2Flow rate of 10L/h, extraction time of 120min, freezing 25kg crude safflower seed oil at 0 deg.C, and centrifuging with 200 mesh filter cloth to obtain 23kg refined safflower seed oil and 37.5kg safflower seed cake and 1.5kg safflower seed oil residue, wherein the refined safflower seed oil is filled into 500ml bottles, and the safflower seed cake and safflower seed residue are mixedFiltering the safflower seed oil residue for later use; the refined safflower seed oil of the embodiment is directly used as edible oil for daily eating.
(3) The safflower seed cake dregs of 37.5kg prepared in the above steps are boiled and washed by ethanol/ethyl acetate according to the mixing ratio of 2:1, the mass ratio of the boiled and washed liquid to the cake dregs is 1.5, 75kg in total, the boiling and washing temperature is 50 ℃, the boiling and washing time is 100min, and the safflower seed protein boiled and washed liquid of 65kg and safflower seed tissue protein of 42kg are obtained by centrifugal filtration by a filter cloth of 200 meshes while the safflower seed protein boiled and washed liquid is hot;
(4) extracting 42kg of safflower seed tissue protein with mixed solution of sodium carbonate and sodium bicarbonate at pH of 9 for 60min and extraction temperature of 40 deg.C under stirring; centrifuging with 200 mesh filter cloth, adjusting pH to 3.0 with acetic acid, standing at 20 deg.C for 16 hr, centrifuging with 200 mesh filter cloth, vacuum freeze drying the precipitate at-20 deg.C under vacuum degree of 60Pa to obtain safflower seed protein isolate 30kg, pulverizing to 100 mesh, and packaging with 5kg bags; the safflower seed separated egg of the embodiment can be added with conventional auxiliary materials and prepared into clinically or commercially acceptable tablets, capsules, powders, mixtures, pills, granules, solutions, syrups, soft extracts, beverages, biscuits, candies or cakes, puffed foods, vegetable protein beverages, blend oils, health care oils, massage oils, daily nuts, pressed candies, oroxylum films and the like according to a conventional process.
(5) Mixing 65kg of safflower seed protein boiled lotion prepared in the step (3) and 1.5kg of safflower seed oil filter residue prepared in the step (2), placing the mixture in a homogenizing emulsifier, homogenizing and stirring for 1h, passing the mixture through a column, mixing the safflower seed shell powder prepared in the step (1) and diatomite according to a ratio of 2:1 to obtain a column filler, consuming 5kg of safflower seed shell powder and 5kg of diatomite together, wherein the inner diameter of the column is 100mm, the height of the column is 1000mm, the outlet flow rate of the column is 80m L/min, carrying out vacuum concentration (vacuum degree of-0.09 MPa and temperature of 30-40 ℃) on the filtrate for 18h, carrying out vacuum freeze drying (vacuum degree of 30-50 Pa and temperature of-30-25 ℃) to obtain 800g of safflower seed 5-hydroxytryptamine derivatives, and filling 100g of each bag for standby, wherein the safflower seed 5-hydroxytryptamine derivatives can be added with conventional auxiliary materials and prepared into clinically or commercially acceptable tablets, pills, powder, tablets, granules, solutions, syrups, decoction, beverages, drinks, massage paste, health-food, cakes, health-care oil, cakes, candies, and the like according to the conventional process.
(6) And (3) after the column is passed through in the step (5), collecting the filler residues (such as safflower seed shell powder, kieselguhr and the like) of the column intensively, and airing and air-drying the collected filler residues in open lands to obtain 9kg of filler residues, wherein the residues can be used for cultivation of high-end edible fungi, preparation of organic fertilizers, soil improvement and the like.
Example 4
Referring to FIG. 1, the comprehensive preparation method of safflower seed multi-product of this example comprises the following steps:
(1) taking 50kg of safflower seed raw materials, washing for 10min by a high-pressure water gun, drying for 4 hours at 50 ℃ by a hot air circulation drying box, carrying out hulling treatment by a special huller to obtain about 12kg of safflower seed shells and about 30kg of safflower seed kernels, crushing the safflower seed shells into 200 meshes by an ultrafine pulverizer to obtain 11kg of safflower seed shell ultrafine powder, and filling 5kg of safflower seed shells into each bag for later use;
(2) grinding 30kg of semen Carthami with colloid mill to particle size of 0.3mm, placing in supercritical extraction equipment, extracting with carbon dioxide supercritical extraction at 35 deg.C under 350MPa with CO as extractant2The flow rate is 20L/h, the extraction time is 180min, 12kg of crude safflower seed oil is obtained, after freezing at the temperature of minus 15 ℃, the crude safflower seed oil is centrifugally filtered by 300-mesh filter cloth, 11kg of refined safflower seed oil, 16.5kg of safflower seed cake and 0.6kg of safflower seed oil filter residue are obtained, 500ml of refined safflower seed oil is filled in each bottle, and the safflower seed cake and the safflower seed oil filter residue are reserved, and the refined safflower seed oil in the embodiment is directly used as edible oil for daily use.
(3) Boiling and washing 16.5kg of safflower seed cake prepared in the above step by using ethanol/ethyl acetate according to the mixing ratio of 1:1.5, wherein the mass ratio of the boiling and washing liquid to the cake is 1.8 and 30kg in total, the boiling and washing temperature is 50 ℃, the boiling and washing time is 100min, and after boiling and washing, the hot safflower seed protein cake is centrifugally filtered by using a filter cloth of 200 meshes to obtain 25kg of safflower seed protein boiling and washing liquid and 20kg of safflower seed tissue protein;
(4) extracting 20kg of safflower seed tissue protein with mixed solution of sodium carbonate and sodium bicarbonate at pH of 9.5 for 90min and extraction temperature of 55 deg.C under stirring; centrifuging with 160 mesh filter cloth, adjusting pH to 3.0 with acetic acid, standing at 20 deg.C or below for 20 hr, centrifuging with 300 mesh filter cloth, vacuum freeze drying the precipitate at-30 deg.C under vacuum degree of 300Pa for 16 hr to obtain safflower seed protein isolate 16kg, pulverizing to 80 mesh, and packaging into 4kg bags; the safflower seed separated egg of the embodiment can be added with conventional auxiliary materials and prepared into clinically or commercially acceptable tablets, capsules, powders, mixtures, pills, granules, solutions, syrups, soft extracts, beverages, biscuits, candies or cakes, puffed foods, vegetable protein beverages, blend oils, health care oils, massage oils, daily nuts, pressed candies, oroxylum films and the like according to a conventional process.
(5) Mixing 25kg of safflower seed protein boiled lotion prepared in the step (3) and 0.6kg of safflower seed oil filter residue prepared in the step (2), placing the mixture in a homogenizing emulsifier, homogenizing and stirring for 40min, passing the mixture through a column, mixing the safflower seed shell powder prepared in the step (1) and diatomite according to a ratio of 1:1 to obtain a column filler, consuming 5kg of safflower seed shell powder and 5kg of diatomite together, wherein the inner diameter of the column is 100mm, the height of the column is 1000mm, the outlet flow rate of the column is kept at 90m L/min, carrying out vacuum concentration (vacuum degree of-0.09 MPa and temperature of 30-40 ℃) on the filtrate for 15h, carrying out vacuum freeze drying (vacuum degree of 30-50 Pa and temperature of-30-25 ℃) to obtain 450g of safflower seed 5-hydroxytryptamine derivatives, and filling 100g of each bag for standby, wherein the safflower seed 5-hydroxytryptamine derivatives can be added with conventional auxiliary materials and prepared into clinically or commercially acceptable tablets, pills, powder, tablets, granules, solutions, syrups, decoction, beverages, drinks, massage paste, health-care food oil, cakes, candy, puffed food oil, cakes, candies and the like according to the conventional process.
(6) And (3) after the column is passed through in the step (5), collecting the filler residues (such as safflower seed shell powder, kieselguhr and the like) of the column intensively, and airing and air-drying the collected filler residues in open lands to obtain 9kg of filler residues, wherein the residues can be used for cultivation of high-end edible fungi, preparation of organic fertilizers, soil improvement and the like.
Example 5
Referring to FIG. 1, the comprehensive preparation method of safflower seed multi-product of this example comprises the following steps:
(1) taking 200kg of safflower seed raw material, washing for 30min by a high-pressure water gun, drying for 4 hours at 55 ℃ by a hot air circulation drying box, carrying out hulling treatment by a special huller to obtain about 50kg of safflower seed shells and about 130kg of safflower seed kernels, crushing the safflower seed shells into 200 meshes by an ultrafine pulverizer to obtain 48kg of safflower seed shell ultrafine powder, and filling 5kg of safflower seed shells into each bag for later use;
(2) grinding 130kg of semen Carthami with colloid mill to particle size of 0.5mm, placing in supercritical extraction equipment, extracting with carbon dioxide supercritical extraction at 40 deg.C under 40MPa with CO as extractant2The flow rate is 25L/h, the extraction time is 300min, 50kg of crude safflower seed oil is obtained, after freezing at the temperature of minus 10 ℃, the crude safflower seed oil is centrifugally filtered by 300-mesh filter cloth, 46kg of refined safflower seed oil and 72kg of safflower seed cake and 2.0kg of safflower seed oil filter residue are obtained, 1000ml of refined safflower seed oil is filled in each bottle, and the safflower seed cake and the safflower seed oil filter residue are reserved, and the refined safflower seed oil in the embodiment is directly used as edible oil for daily use.
(3) Boiling and washing 72kg of safflower seed cake prepared in the above step by using ethanol/ethyl acetate according to the mixing ratio of 1.5:1, wherein the mass ratio of the boiling washing liquid to the cake is 2.0 and 145kg in total, the boiling and washing temperature is 60 ℃, the boiling and washing time is 120min, and after boiling and washing, the safflower seed protein boiling and washing liquid is centrifugally filtered by using a filter cloth of 200 meshes while the safflower seed protein is hot to obtain 130kg of safflower seed protein boiling and washing liquid and 82kg of safflower seed tissue protein;
(4) extracting 82kg of safflower seed tissue protein with mixed solution of sodium carbonate and sodium bicarbonate at pH of 9.0 for 120min and extraction temperature of 60 deg.C under stirring; centrifuging with 160 mesh filter cloth, adjusting pH to 3.9 with acetic acid, standing at 20 deg.C or below for 20 hr, centrifuging with 300 mesh filter cloth, vacuum freeze drying the precipitate at-15 deg.C under 100Pa to obtain safflower seed protein isolate 68kg, pulverizing to 80 mesh, and packaging with 5kg bags; the safflower seed separated egg of the embodiment can be added with conventional auxiliary materials and prepared into clinically or commercially acceptable tablets, capsules, powders, mixtures, pills, granules, solutions, syrups, soft extracts, beverages, biscuits, candies or cakes, puffed foods, vegetable protein beverages, blend oils, health care oils, massage oils, daily nuts, pressed candies, oroxylum films and the like according to a conventional process.
(5) Mixing 130kg of safflower seed protein boiled lotion prepared in the step (3) and 2.0kg of safflower seed oil filter residue prepared in the step (2), placing the mixture in a homogenizing emulsifier, homogenizing and stirring for 60min, passing the mixture through a column, mixing the safflower seed shell powder prepared in the step (1) and diatomite according to a ratio of 2:1 to obtain a column filling material, consuming 6kg of safflower seed shell powder and 3kg of diatomite together, wherein the inner diameter of the column is 100mm, the height of the column is 1000mm, the flow rate of the column outlet is 120m L/min, carrying out vacuum concentration (the vacuum degree is-0.09 MPa, the temperature is 30-40 ℃) for 20h to obtain 1500g of safflower seed 5-hydroxytryptamine derivatives, and filling 100g of each bag for standby, wherein the chemical structure diagram of the prepared safflower seed 5-hydroxytryptamine derivatives is shown in figure 2, the components of the safflower seed 5-hydroxytryptamine derivatives mainly comprise 5-hydroxytryptamine, 5-hydroxytryptamine derivatives such as ferulomine, 5-hydroxytryptamine derivatives, and other conventional dietary supplements, wherein the dietary preparations can be prepared according to the conventional dietary preparations, tablet.
(6) And (3) after the column is passed through in the step (5), collecting the filler residues (such as safflower seed shell powder, kieselguhr and the like) of the column intensively, and airing and air-drying the collected filler residues in open lands to obtain 8kg of filler residues, wherein the residues can be used for cultivation of high-end edible fungi, preparation of organic fertilizers, soil improvement and the like.
The technical solution of the present invention is not limited to the limitations of the above specific embodiments, and all technical modifications made according to the technical solution of the present invention fall within the protection scope of the present invention.
Claims (10)
1. The comprehensive preparation method of the safflower seed multi-product is characterized by comprising the following steps:
s1, washing safflower seeds with water, drying in a hot air circulation drying oven, shelling with a safflower seed sheller to obtain safflower seed shells and safflower seed kernels, pulverizing the safflower seed shells into safflower seed shell powder with an ultrafine pulverizer, and bagging for later use;
s2, grinding the safflower seeds prepared in the step S1 by using a colloid mill, placing the ground safflower seeds into supercritical extraction equipment, and extracting crude safflower seed oil by using a carbon dioxide supercritical extraction technology; freezing the crude safflower seed oil, centrifuging, filtering to obtain refined safflower seed oil, safflower seed cake and safflower seed oil residue, and bottling;
s3, boiling and washing the safflower seed cake prepared in the step S2 with a mixed solution of ethanol and ethyl acetate, centrifuging and filtering while the solution is hot after boiling and washing to obtain a safflower seed protein boiling and washing solution and safflower seed tissue protein;
s4, extracting the safflower seed histones prepared in the step S3 by using a mixed solution of sodium carbonate and sodium bicarbonate; centrifuging and filtering after extraction, adjusting pH of the filtrate with acetic acid, standing, centrifuging and filtering, vacuum freeze drying the obtained precipitate to obtain safflower seed protein isolate, pulverizing, and bagging;
s5, placing the safflower seed protein boiling lotion prepared in the step S3 and the safflower seed oil filter residue prepared in the step S2 into a homogenizing emulsifier for homogenizing and stirring, carrying out column passing on the mixed solution, concentrating the filtrate, carrying out vacuum freeze drying to prepare a safflower seed 5-hydroxytryptamine derivative, and bagging for later use;
and S6, collecting the residues of the column fillers after the column is passed through in the step S5, airing the collected residues in an open field for standby, and using the residues for cultivation of edible fungi, preparation of organic fertilizers and soil improvement.
2. The process for the comprehensive preparation of safflower seed multi-products as claimed in claim 1, wherein: in the step S1, the drying temperature of the safflower seeds in the hot air circulation drying box is 30-60 ℃, the drying time is 2-6 hours, and the granularity of the safflower seed shell powder is 100-300 meshes.
3. The process for the comprehensive preparation of safflower seed multi-products as claimed in claim 1, wherein: in the step S2, the safflower seeds are ground by a colloid mill to have the granularity of 0.1-0.6mm, the supercritical extraction temperature of carbon dioxide is 20-60 ℃, the extraction pressure is 10-50 MPa, and the extractant CO is2The flow rate is 5-40L/h, the extraction time is 60-300 min, the freezing temperature of the crude safflower seed oil is-20-0 ℃, and the crude safflower seed oil is frozen and then is centrifugally filtered by filter cloth of 100-300 meshes.
4. The process for the comprehensive preparation of safflower seed multi-products as claimed in claim 1, wherein: and step S3, mixing the ethanol and ethyl acetate according to the volume ratio of 1: 3-3: 1 to obtain the mixed solution, wherein the mass ratio of the mixed solution of ethanol and ethyl acetate to the safflower seed cake is 1.0-2.0, the boiling and washing temperature is 40-70 ℃, the boiling and washing time is 30-150 min, and the hot mixed solution is subjected to centrifugal filtration by using 100-300-mesh filter cloth after the boiling and washing.
5. The process for the comprehensive preparation of safflower seed multi-products as claimed in claim 1, wherein: the pH value of the mixed solution of sodium carbonate and sodium bicarbonate in the step S4 is 8-10, the extraction time is 30-120 min, the extraction temperature is 30-60 ℃, and the stirring is continuously carried out during the extraction; and after extraction, carrying out centrifugal filtration by using 100-300-mesh filter cloth, adding acetic acid into filtrate to adjust the pH value to 2.0-4.0, standing for 12-24 h below 20 ℃, carrying out centrifugal filtration by using 100-300-mesh filter cloth, carrying out vacuum freeze drying on the obtained precipitate at the vacuum degree of 30-100 Pa and the temperature of-30-15 ℃ for 12-24 h to obtain safflower seed protein isolate, and crushing the safflower seed protein isolate into 60-100 meshes.
6. The comprehensive preparation method of safflower seed multi-product according to claim 1, wherein the homogenizing and stirring time of step S5 is 0.5-2 h, the column packing is prepared by mixing safflower seed shell powder and diatomite according to the mass ratio of 1: 3-3: 1, the inner diameter of the column is 100mm, the height of the column is 1000mm, and the flow rate is 60-120 m L/min.
7. A powdered safflower seed husk obtained by the comprehensive production method of a safflower seed multi-product according to claim 1.
8. A safflower seed oil obtained by the comprehensive production method of the safflower seed multi-product according to claim 1.
9. An isolated protein of safflower seed obtained by the comprehensive production method of safflower seed multi-product as claimed in claim 1.
10. A5-hydroxytryptamine derivative of safflower seed obtained by the comprehensive production method of safflower seed multi-product as claimed in claim 1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911341706.3A CN111466479A (en) | 2019-12-24 | 2019-12-24 | Comprehensive preparation method of safflower seed multi-product |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911341706.3A CN111466479A (en) | 2019-12-24 | 2019-12-24 | Comprehensive preparation method of safflower seed multi-product |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111466479A true CN111466479A (en) | 2020-07-31 |
Family
ID=71746245
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911341706.3A Pending CN111466479A (en) | 2019-12-24 | 2019-12-24 | Comprehensive preparation method of safflower seed multi-product |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111466479A (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103030583A (en) * | 2011-10-08 | 2013-04-10 | 殷越 | Extraction process of 5-hydroxytryptamine derivatives |
| CN104606164A (en) * | 2015-02-04 | 2015-05-13 | 青海民族大学 | Preparation method of flos carthami essence capsule |
| CN109456387A (en) * | 2018-11-27 | 2019-03-12 | 乌鲁木齐上善元生物科技有限公司 | A kind of kardiseed process for producing protein powder |
-
2019
- 2019-12-24 CN CN201911341706.3A patent/CN111466479A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103030583A (en) * | 2011-10-08 | 2013-04-10 | 殷越 | Extraction process of 5-hydroxytryptamine derivatives |
| CN104606164A (en) * | 2015-02-04 | 2015-05-13 | 青海民族大学 | Preparation method of flos carthami essence capsule |
| CN109456387A (en) * | 2018-11-27 | 2019-03-12 | 乌鲁木齐上善元生物科技有限公司 | A kind of kardiseed process for producing protein powder |
Non-Patent Citations (7)
| Title |
|---|
| SATO H, KAWAGISHI H, NISHIMURA T: "Serotobenine, anovel phenolic amide from safflower seeds (Carthamus tinctorius L.)", 《AGRIC BIOL CHEM》 * |
| 张弓，姜明，林鹏程: "超临界CO2萃取河湟红花籽油的工艺研究及GC-MS分析", 《江苏农业科学》 * |
| 张弓，王吉成，吉守祥: "河湟红花籽粕中5-羟色胺衍生物提取方案的优选", 《江苏农业科学》 * |
| 沈飞等: "红花籽粕的综合利用", 《今日科苑》 * |
| 沙希迪: "《食用油脂产品 产品与应用 贝雷油脂化学与工艺学 第4卷 第6版》", 30 November 2016, 中国轻工业出版社 * |
| 荣瑞芬，闫文杰: "《食品科学与工程综合实验指导》", 31 December 2018, 中国轻工业出版社 * |
| 黄凤洪: "《特种油料加工与综合利用》", 31 March 2001, 中国农业科技出版社 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101617787B (en) | Process for continuously extracting a plurality of products from highland barley | |
| CN101791119B (en) | Chaga nutrient composition | |
| CN101948897B (en) | Method for extracting shinyleaf yellowhorn oil, shinyleaf yellowhorn polysaccharide and shinyleaf yellowhorn protolysate peptide from shinyleaf yellowhorn | |
| CN104706821B (en) | A kind of health-care camellia oil | |
| CN106889626A (en) | A kind of apple water-soluble dietary fiber meal replacement powder and preparation method thereof | |
| CN104886568A (en) | Wild jujube seed oil soft capsule production technology | |
| CN105996028B (en) | Ginger extract, ginger fiber and preparation method of ginger extract and ginger fiber | |
| CN110584130A (en) | Preparation method of fructus cannabis protein powder containing water-soluble cannabidiol | |
| CN106978459A (en) | A kind of shiny-leaved yellowhorn protein peptides and preparation method thereof, protein peptide health product | |
| CN101647571B (en) | Compound nutritious composition of edible fungi for regulating blood fat, blood pressure and blood sugar | |
| CN101647555B (en) | Edible fungus composite nutrient composition for improving cardiovascular and cerebrovascular functions | |
| CN1226940C (en) | Royal jelly composition with improved sleep function | |
| CN102960807A (en) | Method for preparing coix seed polysaccharide beverage | |
| CN104774690B (en) | A kind of production technology of fruit of Chinese wolfberry essential oil and edible dregs of rice powder | |
| CN103689119B (en) | A kind of have supplementing the kidney to control the nocturnal and to calm the nerves the Health-care sesame oil of effect | |
| CN103431378A (en) | Composition comprising water-soluble plukenetia volubilis linneo protein powder and preparation method of composition | |
| CN111466479A (en) | Comprehensive preparation method of safflower seed multi-product | |
| CN101664187B (en) | Deer essence nutritional composition | |
| CN103750302A (en) | Lycium chinense alcohol extract as well as preparation method and application thereof | |
| KR101837067B1 (en) | A method for preparing an extract or the powder of steamed Dendropanax morbifera showing abundant total chlorophyll component and quercetin component and the health functional food comprising the an extract or the powder prepared thereby | |
| CN107736542A (en) | A kind of compound lozenge of blueberry and preparation method thereof | |
| KR20170107936A (en) | A method for preparing an extract or the powder of non-steamed Dendropanax morbifera showing abundant total chlorophyll component and quercetin component and the health functional food comprising the an extract or the powder prepared thereby | |
| CN103431100A (en) | Asparagus drink with health protection and health maintenance functions | |
| CN111743005A (en) | Sorbus commixta dietary fiber milk tablet and preparation method thereof | |
| CN104939092A (en) | Composite pericarp extract product having heat clearing and lung moistening efficacy |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20200731 |
|
| RJ01 | Rejection of invention patent application after publication |