CN103030583A - Extraction process of 5-hydroxytryptamine derivatives - Google Patents
Extraction process of 5-hydroxytryptamine derivatives Download PDFInfo
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- CN103030583A CN103030583A CN 201110299588 CN201110299588A CN103030583A CN 103030583 A CN103030583 A CN 103030583A CN 201110299588 CN201110299588 CN 201110299588 CN 201110299588 A CN201110299588 A CN 201110299588A CN 103030583 A CN103030583 A CN 103030583A
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- hydroxytryptamine
- serotonin
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Abstract
The invention relates to an extraction process of 5-hydroxytryptamine derivatives, belonging to the field of fine chemical engineering. The extraction process of 5-hydroxytryptamine derivatives provided by the invention is short in time consumption and high in purity and extraction rate. The invention has the technical scheme that 5-hydroxytryptamine is extracted by taking safflower seeds as raw material, and the method comprises the steps of: adding absolute ethyl alcohol with the material-liquid ratio of (1: 2)-(1: 3) into the safflower seeds, and carrying out reflux extraction for 2.5-3.5h at the temperature of 70-80 DEG C; filtering and then drying the filtrate by evaporation; adding an ethanol solution with the concentration of 80% into the product for dissolving, and then extracting by n-hexane; standing still, carrying out liquid separation, taking methanol phase and drying by evaporation; adding ethyl acetate for dissolving, and then extracting with water; and standing still, carrying out liquid separation, taking ethyl acetate phase and drying by evaporation to obtain the 5-hydroxytryptamine derivatives.
Description
Technical field:
The invention belongs to field of fine chemical, more particularly, relate to the extraction process of serotonin derivative.
Background technology:
The serotonin derivative is a kind of Benzazole compounds, has extremely strong oxidation-resistance and removes free radical activity; The serotonin derivative can balance the body in immune response, suppress tumour; Serotonin derivative asafoetide acyl serotonin and coumaric acyl serotonin have the function of cardioprotection, can promote cardiac motion, prevention myocardial performance generation obstacle.Therefore, the serotonin derivative is a kind of important natural compounds.
Yet traditional extracting method, length consuming time, extraction yield, purity are low, are unfavorable for amplifying producing.
Summary of the invention:
The present invention is exactly for the problems referred to above, and the extraction process of a kind of weak point consuming time, purity, serotonin derivative that extraction yield is high is provided.
In order to realize above-mentioned purpose of the present invention, the present invention adopts following technical scheme, carry out the extraction of serotonin take Semen Flos Carthami as raw material, processing step is: add solid-liquid ratio in the Semen Flos Carthami and be 1: 2～1: 3 dehydrated alcohol, 70～80 ℃ of lower refluxing extraction 2.5～3.5h; After the filtration, with the filtrate evaporate to dryness; Add concentration and be 80% dissolve with ethanol solution, extracts with normal hexane after the dissolving, leave standstill, separatory, get the methyl alcohol phase, evaporate to dryness; Add acetic acid ethyl dissolution, water extraction after the dissolving is left standstill, separatory, gets the ethyl acetate phase, and evaporate to dryness obtains the serotonin derivative.
Beneficial effect of the present invention:
1. the present invention's weak point consuming time, toxicity is little;
2. extraction yield of the present invention is 2.592mmol/g, and the serotonin impurity of extraction is few, need not recrystallization.
Embodiment:
The present invention carries out the extraction of serotonin take Semen Flos Carthami as raw material, processing step is: add solid-liquid ratio in the Semen Flos Carthami and be 1: 2～1: 3 dehydrated alcohol, 70～80 ℃ of lower refluxing extraction 2.5～3.5h; After the filtration, with the filtrate evaporate to dryness; Add concentration and be 80% dissolve with ethanol solution, extracts with normal hexane after the dissolving, leave standstill, separatory, get the methyl alcohol phase, evaporate to dryness; Add acetic acid ethyl dissolution, water extraction after the dissolving is left standstill, separatory, gets the ethyl acetate phase, and evaporate to dryness obtains the serotonin derivative.
The present invention selects solid-liquid ratio, extracts temperature, extraction time is as the factor that affects the serotonin extraction effect, can see Table 1, table 2.
Table 1 three level of factor tables
Table 2 three factors are on the impact of serotonin extraction effect
| Sequence number | A | B | C | Content (mmol/g) |
| 1 | 1 | 3 | 2 | 2.182 |
| 2 | 2 | 1 | 3 | 1.790 |
| 3 | 3 | 2 | 1 | 2.198 |
| 4 | 1 | 2 | 3 | 2.203 |
| 5 | 2 | 3 | 1 | 2.592 |
| 6 | 3 | 1 | 2 | 1.188 |
| 7 | 1 | 1 | 1 | 2.170 |
| 8 | 2 | 2 | 2 | 2.560 |
| 9 | 3 | 3 | 3 | 2.509 |
As a kind of preferred version, processing step of the present invention is: take dehydrated alcohol as extraction agent, solid-liquid ratio is 1: 3, at 80 ℃ of lower 3h that extract.
Claims (2)
1.5-the extraction process of hydroxy-tryptamine derivative, it is characterized in that the present invention adopts following technical scheme, carry out the extraction of serotonin take Semen Flos Carthami as raw material, processing step is: add solid-liquid ratio in the Semen Flos Carthami and be 1: 2～1: 3 dehydrated alcohol, 70～80 ℃ of lower refluxing extraction 2.5～3.5h; After the filtration, with the filtrate evaporate to dryness; Add concentration and be 80% dissolve with ethanol solution, extracts with normal hexane after the dissolving, leave standstill, separatory, get the methyl alcohol phase, evaporate to dryness; Add acetic acid ethyl dissolution, water extraction after the dissolving is left standstill, separatory, gets the ethyl acetate phase, and evaporate to dryness obtains the serotonin derivative.
2. the extraction process of serotonin derivative according to claim 1 is characterized in that, processing step of the present invention is: take dehydrated alcohol as extraction agent, solid-liquid ratio is 1: 3, at 80 ℃ of lower 3h that extract.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110299588 CN103030583A (en) | 2011-10-08 | 2011-10-08 | Extraction process of 5-hydroxytryptamine derivatives |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110299588 CN103030583A (en) | 2011-10-08 | 2011-10-08 | Extraction process of 5-hydroxytryptamine derivatives |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103030583A true CN103030583A (en) | 2013-04-10 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201110299588 Pending CN103030583A (en) | 2011-10-08 | 2011-10-08 | Extraction process of 5-hydroxytryptamine derivatives |
Country Status (1)
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| CN (1) | CN103030583A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103588696A (en) * | 2013-09-06 | 2014-02-19 | 中国科学院西北高原生物研究所 | Method for preparing 5-hydroxytryptamine derivative from safflower seed meal by high-speed countercurrent chromatography |
| CN111466479A (en) * | 2019-12-24 | 2020-07-31 | 德令哈伊明农牧有限公司 | Comprehensive preparation method of safflower seed multi-product |
-
2011
- 2011-10-08 CN CN 201110299588 patent/CN103030583A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103588696A (en) * | 2013-09-06 | 2014-02-19 | 中国科学院西北高原生物研究所 | Method for preparing 5-hydroxytryptamine derivative from safflower seed meal by high-speed countercurrent chromatography |
| CN111466479A (en) * | 2019-12-24 | 2020-07-31 | 德令哈伊明农牧有限公司 | Comprehensive preparation method of safflower seed multi-product |
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| C06 | Publication | ||
| PB01 | Publication | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20130410 |