CN105439846A - Purification method for natural abscisic acid - Google Patents
Purification method for natural abscisic acid Download PDFInfo
- Publication number
- CN105439846A CN105439846A CN201510972956.2A CN201510972956A CN105439846A CN 105439846 A CN105439846 A CN 105439846A CN 201510972956 A CN201510972956 A CN 201510972956A CN 105439846 A CN105439846 A CN 105439846A
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- Prior art keywords
- dormin
- purification
- pbi
- acetone
- ethanol
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C51/00—Preparation of carboxylic acids or their salts, halides or anhydrides
- C07C51/42—Separation; Purification; Stabilisation; Use of additives
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C51/00—Preparation of carboxylic acids or their salts, halides or anhydrides
- C07C51/42—Separation; Purification; Stabilisation; Use of additives
- C07C51/43—Separation; Purification; Stabilisation; Use of additives by change of the physical state, e.g. crystallisation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C51/00—Preparation of carboxylic acids or their salts, halides or anhydrides
- C07C51/42—Separation; Purification; Stabilisation; Use of additives
- C07C51/47—Separation; Purification; Stabilisation; Use of additives by solid-liquid treatment; by chemisorption
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Crystallography & Structural Chemistry (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Abstract
A purification method for natural abscisic acid belongs to the field of separation purification of natural abscisic acid. The method comprises completely dissolving a fermentation abscisic acid crude product with ethanol or an ethanol-water solution or acetone or an acetone-water solution, adding active carbon into the abscisic acid solution for decoloring, keeping warm in water bath, filtering off active carbon after decoloring is finished, collecting the filtrate, supplymenting pure water into the filtrate according to volume, crystallizing with stirring, standing, filtering, collecting precipitate powder, and drying through baking, so as to obtain the abscisic acid product with the content of 93% or more. The method is simple in technological operation, low in production cost, high in efficiency and easy for industrialized application.
Description
Technical field
The invention belongs to the separation and purification field of crude substance, specifically belong to the extracting method of a plant growth regulators.
Background technology
Dormin is a kind of Endogenous hormones with comprehensive physiological function, and being one of five large plant hormones, is the important growth regulator of plant.Dormin can degeneration-resistant immunity system effectively in activated plant body, strengthens the anti-adversity ability under adverse environmental factor, simultaneously
Still grow balance factor, plant Balance Absorption water, fertilizer can be promoted and coordinate the ability of internal metabolism, can the root/be preced with and nourish and grow and reproductive growth of Effective Regulation plant, to quality, the output improving farm crop, there is vital role.Dormin is an extraordinary product of market outlook, and agriculture production has broad application prospects, and can produce huge economic benefit and social benefit.
The extraction of PBI 58 obtained fermenting, purification process are also the focuses of research always, and the general operational path of current method is long, organic solvent usage quantity is large, efficiency is low, cost is high, need to seek a simple and effective method of purification.
Summary of the invention
The present invention is directed to the deficiency that current PBI 58 purifying technique exists, a kind of method of purification of simple and easy, efficient PBI 58 is provided.
Concrete technical scheme of the present invention is as follows.
A method of purification for PBI 58, comprises following steps successively:
(1) dormin crude product ethanol or ethanol-water solution or acetone or acetone-water solution dissolve completely, obtain dormin lysate;
(2) dormin lysate adds the activated carbon decolorizing of 3 ~ 10 ‰, water-bath 40 ~ 60 DEG C insulation 0.5 ~ 2h;
(3) decolouring terminates rear mistake and filters gac, collects filtrate;
(4) filtrate by volume fills into pure water in 1:3 ~ 5, stirred crystallization, leave standstill, filter after collect separate out powder, dry and obtain dormin product.
In step (1), the content of dormin crude product is 70 ~ 85%, the concentration of dormin dissolving crude product is 300 ~ 500mg/ml, ethanol-water solution to be ethanol content be 40 ~ 100% ethanol-water solution, acetone-water solution to be acetone content be 40 ~ 100% acetone-water solution.
In step (4), dormin product content is more than 93%.
Beneficial effect of the present invention is: the present invention is that a kind of purification from the PBI 58 crude product of fermentation gained obtains the method for high purity dormin product, and the method technological operation is simple and easy, production cost is low, efficiency is high, be easy to suitability for industrialized production.
Embodiment
Following examples for illustration of the present invention, but are not used for limiting the scope of the invention.
embodiment 1.
By content be 75% fermentation dormin crude product ethanol dissolve completely, concentration is 450mg/ml, dormin lysate adds the activated carbon decolorizing of 3 ‰, water-bath 45 DEG C insulation 0.8h, decolouring terminates rear mistake and filters gac, collects filtrate, filtrate by volume 1:4 fills into pure water, stirred crystallization, leave standstill, filter after collect separate out powder, drying and obtaining content is 94% dormin product.
embodiment 2.
By content be 80% fermentation dormin crude product 50% ethanol-water solution dissolve completely, concentration is 350mg/ml, dormin lysate adds the activated carbon decolorizing of 5 ‰, water-bath 50 DEG C insulation 1h, decolouring terminates rear mistake and filters gac, collects filtrate, filtrate by volume 1:3 fills into pure water, stirred crystallization, leave standstill, filter after collect separate out powder, drying and obtaining content is 95% dormin product.
embodiment 3.
By content be 83% fermentation dormin crude product 70% ethanol-water solution dissolve completely, concentration is 400mg/ml, dormin lysate adds the activated carbon decolorizing of 10 ‰, water-bath 55 DEG C insulation 1.5h, decolouring terminates rear mistake and filters gac, collects filtrate, filtrate by volume 1:3.5 fills into pure water, stirred crystallization, leave standstill, filter after collect separate out powder, drying and obtaining content is 96% dormin product.
embodiment 4.
By content be 73% fermentation dormin crude product acetone dissolve completely, concentration is 420mg/ml, dormin lysate adds the activated carbon decolorizing of 5 ‰, water-bath 50 DEG C insulation 1.2h, decolouring terminates rear mistake and filters gac, collects filtrate, filtrate by volume 1:4.5 fills into pure water, stirred crystallization, leave standstill, filter after collect separate out powder, drying and obtaining content is 96% dormin product.
embodiment 5.
By content be 80% fermentation dormin crude product 75% acetone-water dissolve completely, concentration is 400mg/ml, dormin lysate adds the activated carbon decolorizing of 8 ‰, water-bath 45 DEG C insulation 1h, decolouring terminates rear mistake and filters gac, collects filtrate, filtrate by volume 1:3 fills into pure water, stirred crystallization, leave standstill, filter after collect separate out powder, drying and obtaining content is 95% dormin product.
embodiment 6.
By content be 84% fermentation dormin crude product 75% acetone-water dissolve completely, concentration is 400mg/ml, dormin lysate adds the activated carbon decolorizing of 5 ‰, water-bath 55 DEG C insulation 0.8h, decolouring terminates rear mistake and filters gac, collects filtrate, filtrate by volume 1:4 fills into pure water, stirred crystallization, leave standstill, filter after collect separate out powder, drying and obtaining content is 96% dormin product.
Claims (8)
1. a method of purification for PBI 58, is characterized in that comprising following steps successively:
(1) dormin crude product ethanol or ethanol-water solution or acetone or acetone-water solution dissolve completely, obtain dormin lysate;
(2) dormin lysate adds activated carbon decolorizing, water bath heat preservation;
(3) decolouring terminates rear mistake and filters gac, collects filtrate;
(4) filtrate by volume fills into pure water, stirred crystallization, leave standstill, filter after collect separate out powder, dry and obtain dormin product.
2. the method for purification of a kind of PBI 58 according to claim 1, is characterized in that ethanol-water solution in step (1) to be ethanol content is the ethanol-water solution of 40 ~ 100%.
3. the method for purification of a kind of PBI 58 according to claim 1, is characterized in that acetone-water solution in step (1) to be acetone content is the acetone-water solution of 40 ~ 100%.
4. the method for purification of a kind of PBI 58 according to claim 1, it is characterized in that the content of dormin crude product in step (1) is 70 ~ 85%, the concentration of dormin dissolving crude product is 300 ~ 500mg/ml.
5. the method for purification of a kind of PBI 58 according to claim 1, is characterized in that the content of gac in step (2) is 3 ~ 10 ‰.
6. the method for purification of a kind of PBI 58 according to claim 1, it is characterized in that in step (2), bath temperature is 40 ~ 60 DEG C, soaking time is 0.5 ~ 2h.
7. the method for purification of a kind of PBI 58 according to claim 1, is characterized in that the volume ratio of pure water and the filtrate filled in step (4) is 3 ~ 5:1.
8., according to the method for purification of described a kind of PBI 58 arbitrary in claim 1 ~ 7, it is characterized in that dormin product content is more than 93%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510972956.2A CN105439846A (en) | 2015-12-23 | 2015-12-23 | Purification method for natural abscisic acid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510972956.2A CN105439846A (en) | 2015-12-23 | 2015-12-23 | Purification method for natural abscisic acid |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105439846A true CN105439846A (en) | 2016-03-30 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510972956.2A Pending CN105439846A (en) | 2015-12-23 | 2015-12-23 | Purification method for natural abscisic acid |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114671754A (en) * | 2022-04-28 | 2022-06-28 | 江西新瑞丰生化股份有限公司 | Method for purifying crude abscisic acid |
| CN116730831A (en) * | 2023-07-03 | 2023-09-12 | 江西新瑞丰生化股份有限公司 | Extraction method of abscisic acid mother liquor |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103274927A (en) * | 2013-06-08 | 2013-09-04 | 南京化工职业技术学院 | Purification method for nature abscisic acid |
| CN104817452A (en) * | 2014-12-19 | 2015-08-05 | 三达膜科技（厦门）有限公司 | Method for extracting abscisic acid from abscisic acid fermentation liquor |
-
2015
- 2015-12-23 CN CN201510972956.2A patent/CN105439846A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103274927A (en) * | 2013-06-08 | 2013-09-04 | 南京化工职业技术学院 | Purification method for nature abscisic acid |
| CN104817452A (en) * | 2014-12-19 | 2015-08-05 | 三达膜科技（厦门）有限公司 | Method for extracting abscisic acid from abscisic acid fermentation liquor |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114671754A (en) * | 2022-04-28 | 2022-06-28 | 江西新瑞丰生化股份有限公司 | Method for purifying crude abscisic acid |
| CN116730831A (en) * | 2023-07-03 | 2023-09-12 | 江西新瑞丰生化股份有限公司 | Extraction method of abscisic acid mother liquor |
| CN116730831B (en) * | 2023-07-03 | 2024-04-12 | 江西新瑞丰生化股份有限公司 | Extraction method of abscisic acid mother liquor |
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Application publication date: 20160330 |
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