CN111436609A

CN111436609A - Product containing plant exosomes and resveratrol and preparation method thereof

Info

Publication number: CN111436609A
Application number: CN202010362287.8A
Authority: CN
Inventors: 吴丹丹; 李珍珍; 曹美霞; 苟晓会; 杜忆华; 靳莉
Original assignee: Dongguan Natural Salutem Health Technology Co ltd
Current assignee: Dongguan Natural Salutem Health Technology Co ltd
Priority date: 2020-04-30
Filing date: 2020-04-30
Publication date: 2020-07-24

Abstract

The invention provides a product containing plant exosomes and resveratrol, which comprises the plant exosomes and the resveratrol, wherein the plant exosomes comprise red grape exosomes, red pomegranate exosomes and carrot exosomes.

Description

Product containing plant exosomes and resveratrol and preparation method thereof

Technical Field

The invention relates to the technical field of food, in particular to a product containing plant exosomes and resveratrol and a preparation method thereof.

Background

With the acceleration of the pace of life, the pressure of people on life and career is increased, more and more people have emotional problems of impatience, anxiety, depression, tension and the like, and meanwhile, smoking, alcoholism, too much fat intake, lack of necessary exercise and pollution of living environment such as radiation, ultraviolet rays, food additives, pesticides and the like sharply reduce the content of negative ions in the air, increase free radicals generated by organisms and directly cause the slow metabolism speed of human bodies.

Free radicals are by-products of the normal aerobic metabolism of living beings, and a small amount of free radicals are beneficial to the human body, and when the concentration of free radicals in the body is not very high, the body is removed by an antioxidant system, but when the body is subjected to external stimuli such as smoking, air pollution, water pollution, radiation (x-ray, ultraviolet ray), pesticides, living pressure and the like, the content of free radicals in the body is increased sharply, and beyond a certain amount, irreversible damage is caused to cells. Since the free radical contains an unpaired electron, it is extremely unstable and takes an electron from a neighboring molecule (fat, protein, DNA) to stabilize itself. The adjacent molecules become a new free radical and then deprive electrons, and the chain reaction is carried out, so that the structure of the cell is damaged, and the cell loses function, mutates genes and even dies.

Scientific studies have shown that aging, cancer or other diseases are mostly associated with the production of excess free radicals. If too many oxidative radicals can be eliminated, aging-related diseases caused by many radicals can be prevented. The antioxidant theory considers that dynamic interaction exists among antioxidants, free radical reaction in organisms is highly related to lipid peroxidation damage, a single antioxidant has limited effect, and the health state of the organism can be maintained only by an organism defense system of an antioxidant complex chain which is composed of different fat solubility and water solubility, different relative molecular mass and different charges.

The drink is popular with consumers due to good taste, but with the continuous improvement of living standard, the demand of people on the drink is not only limited to the satisfaction of the taste, but also the demand on the nutritive value is increasing, wherein the anti-oxidation effect of the drink is concerned.

Patent CN110720573A discloses a beverage with an anti-oxidation function, which mainly comprises the following components by mass: 60-70% of vegetable and fruit concentrated juice, 3-10% of citric acid, 1-3% of procyanidine, 1-4% of salt, 4-10% of honey, 0.5-2% of vitamin complex and 8-15% of maltodextrin, wherein the sum of the total amount of the components is 100%. The vegetable and fruit concentrated juice is one of orange juice, grape juice, corn juice, passion fruit juice and apple juice. 30 g/bag of powder prepared from CN110720573A has large daily dose, and the powder needs to be mixed before being taken, which brings inconvenience to consumers; on the other hand, the preparation process of CN110720573A is more in process, time and labor consuming, cost increasing, and more in nutrient loss.

Therefore, the innovative fruit and vegetable extraction technology is developed, the resveratrol is combined, and the resveratrol is applied to the aspects of safe and effective products containing plant exosomes and resveratrol and having better antioxidant effect, so that the resveratrol has important practical significance and broad market prospect.

Disclosure of Invention

In view of the above, the present invention aims to provide a safe and effective product containing plant exosomes and resveratrol with better antioxidant effect.

Therefore, the invention provides the following technical scheme.

In a first aspect, the invention provides a product comprising plant exosomes and resveratrol, the product comprising plant exosomes comprising red grape exosomes, red pomegranate exosomes and carrot exosomes and resveratrol.

In a preferred embodiment, the product further comprises grape fruit powder, pomegranate concentrate powder, yeast β -glucan, yeast extract, cranberry concentrate, elderberry concentrate, astaxanthin, olive powder, momordica grosvenori concentrate, xanthan gum, potassium sorbate, and water.

In a preferred embodiment, the product comprises 0.5-10 parts of red grape exosomes, 0.5-8 parts of red pomegranate exosomes, 0.1-6 parts of carrot exosomes and 0.1-3 parts of resveratrol by weight.

In a preferred embodiment, the product comprises, in parts by weight:

2-15 parts of grape powder, 0.1-5 parts of pomegranate concentrated powder, 0.1-3 parts of yeast β -glucan, 0.5-5 parts of yeast extract, 0.1-7 parts of cranberry concentrated juice, 1-8 parts of elderberry concentrated juice, 0.01-0.08 part of astaxanthin, 0.1-2 parts of olive powder, 0.1-3 parts of momordica grosvenori concentrated juice, 0.03-0.2 part of xanthan gum, 0.01-0.08 part of potassium sorbate and water.

In a second aspect, the invention provides a product containing plant exosomes and resveratrol, wherein the product is prepared from the following components in parts by weight:

0.5-5 parts of red grape exosome, 2-6 parts of grape fruit powder, 0.1-1 part of resveratrol, 0.5-5 parts of red pomegranate exosome, 0.1-2 parts of red pomegranate concentrated powder, 0.1-1 part of yeast β -glucan, 0.5-2 parts of yeast extract, 0.1-5 parts of carrot exosome, 0.1-5 parts of cranberry concentrated juice, 1-3 parts of elderberry concentrated juice, 0.01-0.05 part of astaxanthin, 0.1-1 part of olive powder, 0.1-0.5 part of momordica grosvenori concentrated juice, 0.03-0.1 part of xanthan gum, 0.01-0.04 part of potassium sorbate and water.

In a third aspect, the invention provides a product containing plant exosomes and resveratrol, wherein the product is prepared from the following components in parts by weight:

5-10 parts of red grape exosome, 6-15 parts of grape fruit powder, 1-3 parts of resveratrol, 5-8 parts of red pomegranate exosome, 2-5 parts of red pomegranate concentrated powder, 1-3 parts of yeast β -glucan, 2-5 parts of yeast extract, 5-6 parts of carrot exosome, 5-7 parts of cranberry concentrated juice, 3-8 parts of elderberry concentrated juice, 0.05-0.08 part of astaxanthin, 1-2 parts of olive powder, 0.5-3 parts of momordica grosvenori concentrated juice, 0.1-0.2 part of xanthan gum, 0.04-0.08 part of potassium sorbate and water.

In a fourth aspect, the invention provides a product containing plant exosomes and resveratrol, wherein the product is prepared from the following components in parts by weight:

5 parts of red grape exosome, 6 parts of grape fruit powder, 1 part of resveratrol, 5 parts of red pomegranate exosome, 2 parts of red pomegranate concentrated powder, 1 part of yeast β -glucan, 2 parts of yeast extract, 5 parts of carrot exosome, 5 parts of cranberry concentrated juice, 3 parts of elderberry concentrated juice, 0.05 part of astaxanthin, 1 part of olive powder, 0.5 part of momordica grosvenori concentrated juice, 0.1 part of xanthan gum, 0.04 part of potassium sorbate and water.

In a fifth aspect, there is provided the use of red grape exosomes, red pomegranate exosomes, carrot exosomes and/or resveratrol or a composition comprising red grape exosomes, red pomegranate exosomes, carrot exosomes and resveratrol in the preparation of a product containing plant exosomes and resveratrol.

In a preferred embodiment of the present invention,

the red grape exosome is prepared by the following method:

s1, adding 200m L PBS buffer solution into 200g of red grapes, crushing for 10min by using a crusher, stopping for 1min every time the crushing is performed for 1min, and obtaining red grape juice;

s2, centrifuging the red grape juice obtained in the step S1 at 4 ℃ for 15min at 3000 × g to obtain supernatant liquid 1;

s3, centrifuging the supernatant liquid 1 obtained in the step S2 at 4 ℃ for 20min at 5000 × g to obtain a supernatant liquid 2;

s4, centrifuging the supernatant liquid 2 obtained in the step S3 at 4 ℃ for 60min at 10000 × g to obtain a supernatant liquid 3, namely the red grape exosome.

In a preferred embodiment of the present invention,

the pomegranate exosome is prepared by the following method:

s1, adding 200m L PBS buffer solution into 200g of red pomegranate, crushing for 10min by using a crusher, stopping crushing for 1min every time the crushing is performed for 1min, and obtaining red pomegranate juice;

s2, centrifuging the pomegranate juice obtained in the step S1 at 4 ℃ for 15min at 3000 × g to obtain supernatant liquid 1;

s4, centrifuging the supernatant liquid 2 obtained in the step S3 at 4 ℃ for 60min at 10000 × g to obtain a supernatant liquid 3, namely the red pomegranate exosome.

In a preferred embodiment of the present invention,

the carrot exosome is prepared by the following method:

s1, adding 200m L PBS buffer solution into 200g of carrots, crushing for 10min by using a crusher, stopping for 1min every time the carrot is crushed for 1min to obtain carrot juice;

s2, centrifuging the carrot juice obtained in the step S1 at 4 ℃ for 15min at 3000 × g to obtain supernatant liquid 1;

s4, centrifuging the supernatant liquid 2 obtained in the step S3 at 4 ℃ for 60min at 10000 × g to obtain a supernatant liquid 3, namely the carrot exosome.

In a sixth aspect, there is provided a process for the preparation of a product containing plant exosomes and resveratrol, the process comprising the steps of:

s1, heating water to 70 ℃ and keeping the temperature, slowly adding xanthan gum under the stirring state, and stirring for 15min until the colloid appearance is clear and transparent to obtain a solution A;

s2, sequentially adding grape fruit powder, resveratrol, pomegranate concentrated powder, yeast β -glucan, yeast extract, astaxanthin, olive powder and potassium sorbate into water under stirring, and adding the next raw material after stirring and dissolving each time to obtain solution B;

s3, mixing the solution A and the solution B, stirring for 5min, sequentially adding red grape exosomes, red pomegranate exosomes, carrot exosomes, cranberry concentrated juice, elderberry concentrated juice and momordica grosvenori concentrated juice, stirring and dissolving each time, and then adding the next raw material to obtain solution C;

s4, homogenizing the solution C for 10min at 3000r/min by using a homogenizer to obtain a product intermediate containing the plant exosome and the resveratrol;

s5, filling the product intermediate containing the plant exosomes and the resveratrol obtained in the step S4 under an aseptic condition, wherein the filling amount is 30g +/-2.7 g per bag;

s6, performing water bath pasteurization on the filled product for 3min at the temperature of 93 ℃ by adopting a pasteurization method.

In a seventh aspect, the invention provides a product containing plant exosomes and resveratrol, wherein the product containing plant exosomes and resveratrol is prepared from the following components:

red grape exosomes, grape fruit powder, resveratrol, red pomegranate exosomes, red pomegranate concentrated powder, yeast β -glucan, yeast extract, carrot exosomes, cranberry concentrated juice, elderberry concentrated juice, astaxanthin, olive powder, momordica grosvenori concentrated juice, xanthan gum, potassium sorbate and water.

In an eighth aspect, there is provided a pharmaceutical composition comprising a safe and effective amount of a product comprising plant exosomes and resveratrol according to the present invention.

In a ninth aspect, the invention provides the use of a product containing plant exosomes and resveratrol in the preparation of a functional food or pharmaceutical composition.

In the invention, the pharmaceutical composition comprises the product containing the plant exosomes and the resveratrol and pharmaceutically acceptable auxiliary materials.

In the present invention, a safe and effective amount refers to an amount of a compound or composition sufficient to significantly induce a positive benefit, preferably a positive antioxidant benefit, including the benefits disclosed herein, either individually or in combination, but low enough to avoid serious side effects, i.e., to provide a reasonable benefit to risk ratio, within the scope of sound judgment of the skilled artisan.

The taking method of the product containing the plant exosomes and the resveratrol comprises the following steps: each bag is 30g +/-2.7 g, and the drink is drunk after being opened once a day.

Red grape exosomes, grape fruit powder:

the grape seed and grape skin aqueous extract has strong scavenging effect on free radicals, and the anti-free radical activity is that fresh grape seeds are larger than grape skin and dry grape seeds are larger than grape skin. The grape contains abundant phenolic active components (procyanidin, resveratrol, anthocyanin, flavone, etc.), and is mainly derived from grape pericarp and seed. Procyanidins are researched and found to be the safest and efficient antioxidant, the antioxidant capacity of the antioxidant is 20 times that of vitamin C and 50 times that of vitamin E, and the content of procyanidins in grape seeds is the highest.

Resveratrol:

the experiments prove that the resveratrol can lower the nitrogen monoxide (NO) and Malondialdehyde (MDA) in brain tissues and serum of mice aged by D-galactose to different degrees, the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) are increased to different degrees, and lipofuscin (L F) in the brain tissues is reduced, so that the resveratrol can delay aging by removing oxygen free radicals and resisting lipid peroxidation.

Red pomegranate exosomes and red pomegranate concentrated powder:

pomegranate fruit, each of which is rich in potent antioxidants, vital vitamins and minerals. Pomegranate contains abundant antioxidant bioactive substances such as tannin, anthocyanins, flavonoids, protocatechuic acid, ellagic acid, etc. The pomegranate polyphenols and anthocyanin are known as two great longitudes in the antioxidant world, and can thoroughly expel toxic substances of harmful free radicals causing skin darkness, wilting yellow and exhaustion and recover the purity of the skin. The ellagic acid component contained in the pomegranate is the most known component with the anti-aging effect for human beings, and the ellagic acid can nourish cells, tighten the skin, reduce inflammation sensitivity and provide moisture for the skin. In addition, pomegranate fruit contains high amounts of potassium and phosphorus, polyphenols, linoleic acid and various minerals. Vitamin C, vitamin B6, vitamin E, folic acid, mineral calcium, magnesium and zinc in the pomegranate fruit can rapidly inject abundant fresh energy into the skin, so that the skin is revived and active and gives out natural healthy luster.

Yeast β -dextran:

the yeast β -glucan is extracted and purified by saccharomyces cerevisiae, and mainly comprises β -1,3/1 and 6-glucan, can provide multiple functions, such as antioxidation, immunity enhancement, infection resistance, tumor resistance, radiation resistance and blood fat regulation, the yeast glucan is the glucan which is found to have immunological activity for the first time, β -glucan can activate macrophages, neutrophils and the like, so that the content of leucocyte, cytokinin and special antibody can be increased, the immune system of the organism can be comprehensively stimulated, meanwhile, the glucan has a good repairing effect on damaged cells, β -glucan can enable the damaged lymphocytes of the organism to rapidly recover the normal ability of producing cell factors (I L-1), and can effectively regulate the immune function of the organism, the production of protective antibodies (IgM antibodies) with immunity in the organism can be promoted, so that the immune capability of body fluid is improved, β -glucan can effectively remove free radicals in the organism, so that the macrophages can be prevented from being attacked by the free radicals after being radiated, and the macrophages can continuously play a role.

Yeast extract (containing glutathione):

glutathione is a natural active peptide with important physiological functions, consists of glutamic acid, cysteine and glycine, exists in 2 forms in vivo, namely reduced Glutathione (GSH) and oxidized glutathione (GSSG), and exists in large amount in the body and plays a main role in reducing glutathione. Glutathione GSH has functions of scavenging free radicals, resisting oxidation and resisting aging. GSH can eliminate redundant free radicals in the organism and play a role in strong protection, because the structure of the glutathione GSH contains an active sulfhydryl-SH which is easy to be oxidized and dehydrogenated, the specific structure can be used as a main free radical scavenger in the organism. Glutathione GSH not only can remove free radicals of human body, but also can improve immunity of human body.

Carrot exosomes:

the carrot not only can enhance the immunity of a human body and has an anticancer effect, but also contains rich carotene, and the carotene can remove singlet oxygen and free radicals causing human aging, slow down the aging process of the human body and prevent skin aging.

Cranberry concentrated juice:

the cranberry contains various antioxidant and anti-aging bioactive substances, flavonoid compounds in the cranberry have antioxidant functions in vivo and in vitro tests, anthocyanin is a dark red pigment contained in the cranberry and other berries (such as blueberries, cherries and the like), is also a very strong antioxidant, has the antioxidant capacity even higher than that of vitamin C and vitamin E, is rich in vitamin C with the content of 13.5mg/100g, has very strong reducibility, and also contains β -carotene, and can capture free radicals and reduce the oxidative damage of the free radicals.

Bone-knitting raspberry concentrated juice:

the elderberry is the fruit of elderberry, is rich in anthocyanin, namely vitamin C, and thus becomes a super fruit for supporting immune function. The main chemical composition comprises flavanol (32.8mg/100g), anthocyanin (485mg/100g), and natural vitamin (36mg/100 g). The elderberry is rich in anthocyanin, and can protect endothelial cells from oxidation loss by removing free radicals, thereby achieving the effect of antioxidation.

Astaxanthin:

astaxanthin is widely existed in the nature, is one of the strongest natural antioxidants in the world, effectively eliminates oxygen free radicals in cells, enhances the cell regeneration capacity, maintains the organism balance and reduces the accumulation of aging cells, protects the cells and DNA health from inside to outside, thereby protecting the skin health, promoting the hair growth, resisting aging, relieving sports fatigue and enhancing the activity, and the capacity of eliminating free radicals is 6000 times of the efficacy of vitamin C, 1000 times of vitamin E, 800 times of coenzyme Q10, 700 times of anthocyanin, 100 times of the efficacy of β -carotene, 10 times of lycopene, 200 times of lutein and 320 times of tea polyphenol.

Olive powder:

the fruit pulp contains protein, carbohydrate, fat, vitamin C, calcium, phosphorus, iron and other minerals, and the active substances include olive polyphenol, oleuropein and hydroxytyrosol, and have antioxidant and ultraviolet aging preventing effects, and can effectively inhibit pigmentation, fade color spots and brighten skin. Hydroxytyrosol is a polyphenol which is recognized by the scientific community and has strong antibacterial, anti-inflammatory, antioxidant and heart health protection functions.

Fructus momordicae concentrated juice:

the nutrient content is 7.2% of protein and the content of crude protein is about 9%. Glucose and fructose are about 14%. D-mannose (fresh fruit content) 0.08%. Mogroside IV has no sweet taste and contains multiple vitamins. Contains a large amount of vitamin C, and can help to eliminate free radicals in vivo and delay aging.

Compared with the prior art, the invention has the beneficial effects that:

the plant exosome is obtained by the secretion of plant cells, contains most of protein, lipid, RNA, resveratrol, carotenoid and polyphenol active ingredients of donor cells, and has good antioxidant capacity. The red grape exosome, the red pomegranate exosome and the carrot exosome prepared by the method have good effects of scavenging free radicals and resisting oxidation.

Resveratrol can reduce nitrogen monoxide (NO) and Malondialdehyde (MDA) in brain tissue and serum of mice with aging caused by D-galactose to different degrees, increase activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) to different degrees, and reduce lipofuscin (L F) in brain tissue, so resveratrol can delay aging by scavenging oxygen free radicals and resisting lipid peroxidation.

The red grape exosomes, grape fruit powder, resveratrol, red pomegranate exosomes, red pomegranate concentrated powder, carrot exosomes, cranberry concentrated juice, elderberry concentrated juice, astaxanthin and olive fruit powder contained in the product containing the plant exosomes and the resveratrol cooperate with each other through different antioxidant action mechanisms of vitamins, carotenoids, polyphenols, resveratrol, anthocyanins, hydroxytyrosol and the like to achieve multidirectional free radical removal and efficient antioxidant.

Vitamin C exists in plant in reducing form, can react with various active oxygen, and can scavenge-0H, H₂O₂、ROO·、¹O₂The vitamin E is easy to react with oxidation substances so as to protect cells from harmful peroxidation substances generated by oxidation of unsaturated fatty acid, the carotenoid prevents lipid peroxidation from removing active oxygen free radicals through high-activity and electron-rich long polyene carbon chains, the procyanidine prevents the cells from being damaged by peroxidation through inhibiting enzyme activity which destroys important component substances such as collagen, elastin, hyaluronic acid and the like which form the inner wall of blood vessels, conjugated double bonds, hydroxyl groups and unsaturated ketone groups in an astaxanthin molecular structure are more in number, and the hydroxyl groups and the ketone groups form α -hydroxyketone, wherein the structures all have active electronic effects, can attract free radicals to unpaired electrons or provide electrons for the free radicals to remove the free radicals and play an obvious antioxidation effect, the resveratrol activates acetylase I to reduce malondialdehyde generated by lipid peroxidation reaction so as to reduce organism aging, in addition, the invention is compounded with yeast β -glucan and yeast extract to protect macrophages from being attacked by the free radicals and easy to be oxidized and dehydrogenated by sulfhydryl-SH so as to specifically remove the free radicals.

In addition, the product containing the plant exosomes and the resveratrol is rich in various vitamins and mineral substances, β -glucan can promote the generation of IgM antibodies and improve the immunity, and glutathione also has a whitening effect, so that the problem of single product effect is solved.

The pH value of the product containing the plant exosomes and the resveratrol is stably maintained at 3-4, the adding sequence of extracts and fruit juice in raw materials of the product is from sweet to sour, the extracts and the fruit juice are sequentially added from high pH value to low pH value, the used thickening agent is xanthan gum which is very stable to temperature and acid and alkali, the viscosity of an aqueous solution of the xanthan gum is hardly changed between 10 ℃ and 80 ℃, the difference between the maximum value and the minimum value of the viscosity is less than 10 percent in the range of pH3-11, the xanthan gum is quite stable at normal temperature, and the gum can not be changed for months. According to the invention, through stability experiments, microorganisms, color, taste, pH and the like are monitored, and the product is proved to have stable properties within 18 months of shelf life.

The product containing the plant exosomes and the resveratrol has the advantages that the raw materials in the formula are food raw materials or are allowed to be used as functional food raw materials in China, the dosage is strictly screened, the product can be eaten for a long time, and the formula is safe and nontoxic through animal safety verification.

In addition, the product containing the plant exosomes and the resveratrol has good taste, is convenient to carry and is suitable for a wide range of people.

Moreover, the preparation method of the product containing the plant exosomes and the resveratrol has the advantages of simple production process, safety, feasibility and low energy consumption.

Moreover, the product containing the plant exosome and the resveratrol can keep excellent stability from low temperature to high temperature, does not have layering or precipitation and has good stability.

Detailed Description

The present invention will be described in detail below with reference to specific embodiments and examples, and the advantages and various effects of the present invention will be more clearly apparent therefrom. It will be understood by those skilled in the art that these specific embodiments and examples are for the purpose of illustrating the invention and are not to be construed as limiting the invention.

The present invention will be described in more detail below with reference to examples, but the present invention is not limited to these examples.

Example 1

The embodiment provides a product containing plant exosomes and resveratrol, which is prepared from the following components in parts by weight:

0.5 part of red grape exosome, 2 parts of grape fruit powder, 0.1 part of resveratrol, 0.5 part of red pomegranate exosome, 0.1 part of red pomegranate concentrated powder, 0.1 part of yeast β -glucan, 0.5 part of yeast extract, 0.1 part of carrot exosome, 0.1 part of cranberry concentrated juice, 1 part of elderberry concentrated juice, 0.01 part of astaxanthin, 0.1 part of olive powder, 0.1 part of momordica grosvenori concentrated juice, 0.03 part of xanthan gum, 0.01 part of potassium sorbate and water.

Wherein,

the red grape exosome is prepared by the following method:

s4, centrifuging the supernatant liquid 2 obtained in the step S3 at 4 ℃ for 60min at 10000 × g to obtain a supernatant liquid 3, namely the red grape exosome;

the pomegranate exosome is prepared by the following method:

s4, centrifuging the supernatant liquid 2 obtained in the step S3 at 4 ℃ for 60min at 10000 × g to obtain a supernatant liquid 3, namely the red pomegranate exosome;

the carrot exosome is prepared by the following method:

Example 2

10 parts of red grape exosome, 15 parts of grape fruit powder, 3 parts of resveratrol, 8 parts of red pomegranate exosome, 5 parts of red pomegranate concentrated powder, 3 parts of yeast β -glucan, 5 parts of yeast extract, 6 parts of carrot exosome, 7 parts of cranberry concentrated juice, 8 parts of elderberry concentrated juice, 0.08 part of astaxanthin, 2 parts of olive powder, 3 parts of momordica grosvenori concentrated juice, 0.2 part of xanthan gum, 0.08 part of potassium sorbate and water.

Wherein,

the red grape exosome is prepared by the following method:

the pomegranate exosome is prepared by the following method:

the carrot exosome is prepared by the following method:

Example 3

Wherein,

the red grape exosome is prepared by the following method:

the pomegranate exosome is prepared by the following method:

the carrot exosome is prepared by the following method:

Example 4

This example provides a process for the preparation of a product containing plant exosomes and resveratrol as described in example 3, the process comprising the steps of:

s1, heating water to 70 ℃ and keeping the temperature, slowly adding 0.1 part of xanthan gum under stirring, and stirring for 15min until the appearance of a colloid is clear and transparent to obtain a solution A;

s2, under the stirring state, sequentially adding 6 parts of grape fruit powder, 1 part of resveratrol, 2 parts of pomegranate concentrated powder, 1 part of yeast β -glucan, 2 parts of yeast extract, 0.05 part of astaxanthin, 1 part of olive powder and 0.04 part of potassium sorbate into water, adding the next raw material after stirring and dissolving each time, and obtaining a solution B;

s3, mixing the solution A and the solution B, stirring for 5min, sequentially adding 5 parts of red grape exosomes, 5 parts of red pomegranate exosomes, 5 parts of carrot exosomes, 5 parts of cranberry concentrated juice, 3 parts of elderberry concentrated juice and 0.5 part of momordica grosvenori concentrated juice, and adding the next raw material after stirring and dissolving each time to obtain solution C;

s4, homogenizing the solution C for 10min at 3000r/min by using a homogenizer to obtain a product intermediate containing the plant exosomes and the resveratrol;

Example 5

The embodiment provides application of red grape exosomes, red pomegranate exosomes and carrot exosome extracting solutions in preparation of products containing plant exosomes and resveratrol.

Wherein,

the red grape exosome is prepared by the following method:

the pomegranate exosome is prepared by the following method:

the carrot exosome is prepared by the following method:

Example 6

The present example provides the use of a composition comprising red grape exosomes, red pomegranate exosomes and carrot exosomes in the preparation of a product containing plant exosomes and resveratrol.

Wherein,

the red grape exosome is prepared by the following method:

the pomegranate exosome is prepared by the following method:

the carrot exosome is prepared by the following method:

In some embodiments, the composition comprises the following components:

In some embodiments, the composition comprises the following components in parts by weight:

0.5-10 parts of red grape exosome, 2-15 parts of grape fruit powder, 0.1-3 parts of resveratrol, 0.5-8 parts of red pomegranate exosome, 0.1-5 parts of red pomegranate concentrated powder, 0.1-3 parts of yeast β -glucan, 0.5-5 parts of yeast extract, 0.1-6 parts of carrot exosome, 0.1-7 parts of cranberry concentrated juice, 1-8 parts of elderberry concentrated juice, 0.01-0.08 part of astaxanthin, 0.1-2 parts of olive powder, 0.1-3 parts of momordica grosvenori concentrated juice, 0.03-0.2 part of xanthan gum, 0.01-0.08 part of potassium sorbate and water.

Comparative example 1

To further illustrate the beneficial effects of the present invention, comparative example 1 is provided, which is different from example 3 in that: the weight part of the red grape exosomes is adjusted to be 0.3 part.

Comparative example 2

To further illustrate the beneficial effects of the present invention, comparative example 2 is provided, which comparative example 2 differs from example 3 in that: the weight portion of the red grape exosome is adjusted to 0.3 portion, and the weight portion of the red pomegranate exosome is adjusted to 0.4 portion.

Comparative example 3

To further illustrate the beneficial effects of the present invention, comparative example 3 is provided, which comparative example 3 differs from example 3 in that: the weight portion of the red grape exosome is adjusted to 0.3 portion, the weight portion of the red pomegranate exosome is adjusted to 0.4 portion, and the weight portion of the carrot exosome is adjusted to 0.05 portion.

Comparative example 4

To further illustrate the beneficial effects of the present invention, a comparative example 4 is provided, which comprises a product of plant exosomes and resveratrol according to parts by weight, prepared from the following components:

0.4 part of red grape exosome, 1 part of grape fruit powder, 0.05 part of resveratrol, 0.4 part of red pomegranate exosome, 0.05 part of red pomegranate concentrated powder, 0.05 part of yeast β -glucan, 0.4 part of yeast extract, 0.05 part of carrot exosome, 0.05 part of cranberry concentrated juice, 0.5 part of elderberry concentrated juice, 0.005 part of astaxanthin, 0.05 part of olive powder, 0.05 part of momordica grosvenori concentrated juice, 0.02 part of xanthan gum, 0.005 part of potassium sorbate and water.

Wherein,

the red grape exosome is prepared by the following method:

the pomegranate exosome is prepared by the following method:

the carrot exosome is prepared by the following method:

Comparative example 5

To further illustrate the beneficial effects of the present invention, comparative example 5 is provided, which is different from example 3 in that: the weight part of the red grape exosomes is adjusted to 11 parts.

Comparative example 6

To further illustrate the beneficial effects of the present invention, comparative example 6 is provided, which is different from example 3 in that: the weight portion of the red grape exosome is adjusted to 11 portions, and the weight portion of the red pomegranate exosome is adjusted to 9 portions.

Comparative example 7

To further illustrate the beneficial effects of the present invention, comparative example 3 is provided, which comparative example 3 differs from example 3 in that: the weight portion of the red grape exosome is adjusted to 11 portions, the weight portion of the red pomegranate exosome is adjusted to 9 portions, and the weight portion of the carrot exosome is adjusted to 7 portions.

Comparative example 8

To further illustrate the beneficial effects of the present invention, a comparative example 8 is provided, which comprises a product of plant exosomes and resveratrol according to parts by weight, prepared from the following components:

11 parts of red grape exosome, 16 parts of grape fruit powder, 4 parts of resveratrol, 9 parts of red pomegranate exosome, 6 parts of red pomegranate concentrated powder, 4 parts of yeast β -glucan, 6 parts of yeast extract, 7 parts of carrot exosome, 8 parts of cranberry concentrated juice, 9 parts of elderberry concentrated juice, 0.1 part of astaxanthin, 2.5 parts of olive powder, 4 parts of momordica grosvenori concentrated juice, 0.3 part of xanthan gum, 0.1 part of potassium sorbate and water.

Wherein,

the red grape exosome is prepared by the following method:

the pomegranate exosome is prepared by the following method:

the carrot exosome is prepared by the following method:

Comparative example 9

To further illustrate the beneficial effects of the present invention, a comparative example 9 is provided, which comprises the following components in parts by weight:

5 parts of white grape concentrated juice, 6 parts of grape fruit powder, 1 part of resveratrol, 5 parts of red pomegranate exosome, 2 parts of red pomegranate concentrated powder, 1 part of yeast β -glucan, 2 parts of yeast extract, 5 parts of carrot exosome, 5 parts of cranberry concentrated juice, 3 parts of elderberry concentrated juice, 0.05 part of astaxanthin, 1 part of olive powder, 0.5 part of momordica grosvenori concentrated juice, 0.1 part of xanthan gum, 0.04 part of potassium sorbate and water.

Wherein,

the pomegranate exosome is prepared by the following method:

the carrot exosome is prepared by the following method:

Comparative example 10

To further illustrate the beneficial effects of the present invention, a comparative example 10 is provided, which comprises a product of plant exosomes and resveratrol according to parts by weight, prepared from the following components:

5 parts of white grape concentrated juice, 6 parts of grape fruit powder, 1 part of resveratrol, 5 parts of red pomegranate exosome, 2 parts of red pomegranate concentrated powder, 1 part of yeast β -glucan, 2 parts of yeast extract, 5 parts of carrot exosome, 5 parts of blueberry concentrated juice, 3 parts of elderberry concentrated juice, 0.05 part of astaxanthin, 1 part of olive powder, 0.5 part of momordica grosvenori concentrated juice, 0.1 part of xanthan gum, 0.04 part of potassium sorbate and water.

Wherein,

the pomegranate exosome is prepared by the following method:

the carrot exosome is prepared by the following method:

Comparative example 11

To further illustrate the beneficial effects of the present invention, a comparative example 11 is provided, which comprises a product of plant exosomes and resveratrol according to parts by weight, prepared from the following components:

5 parts of white grape concentrated juice, 6 parts of grape fruit powder, 1 part of resveratrol, 5 parts of tomato concentrated juice, 2 parts of pomegranate concentrated powder, 1 part of yeast β -glucan, 2 parts of yeast extract, 5 parts of broccoli concentrated juice, 5 parts of cranberry concentrated juice, 3 parts of elderberry concentrated juice, 0.05 part of astaxanthin, 1 part of olive powder, 0.5 part of momordica grosvenori concentrated juice, 0.1 part of xanthan gum, 0.04 part of potassium sorbate and water.

Comparative example 12

In order to further illustrate the beneficial effects of the invention, the beverage with the antioxidant function is provided, and the beverage with the antioxidant function is prepared by adopting the method in CN 110720573A.

Experiment 1: security verification

And (3) testing a sample: examples 1-3 samples prepared

The experimental method comprises the following steps:

10 male and female mice are screened, the male and female mice are raised in cages with the weight of 18-22g, and the product containing the plant exosome and the resveratrol is used for performing an intragastric administration experiment on the mice. After administration, the mice were observed for behavioral activity, mental state, appetite, fur, weight change, and death status on days 0, 7, and 14, respectively.

Through carrying out acute oral toxicity experiments on mice, the experiments are divided into two groups of experiments of a high dose group and a normal group, the observation is carried out for 14 days, and relevant data are recorded on 0 day, 7 days and 14 days respectively, and the experimental data are shown in the following table 1 and the following table 2.

TABLE 1 test results of normal acute oral toxicity group

TABLE 2 Experimental results for acute oral toxicity high dose group

From the above results, it can be seen that:

the product containing the plant exosomes and the resveratrol, which is prepared by the invention, is administrated to experimental animals by gavage at normal dose or high dose, the experimental animals have good mental states, normal behaviors, normal weight increase, no adverse reaction and 0 death number after gavage, and the product containing the plant exosomes and the resveratrol is non-toxic and safe, belongs to a non-toxic result and meets the requirement of acute toxicity experimental results of biological safety evaluation.

Experiment 2: radical scavenging ability verification

And (3) testing a sample: examples 1-3, comparative examples 1-12

The experimental method comprises the following steps:

2m L test sample and 2m L0.1 mmol/L DPPH absolute ethanol solution are addedMixing in the same tube, standing at room temperature in dark for 30min, measuring absorbance at 517nm, and recording as A₁Adding 2m L anhydrous ethanol and 2m L DPPH solution into the same tube, mixing, standing at room temperature in dark for 30min, measuring absorbance at 517nm, and recording as A₀Adding 2m L test sample and 2m L anhydrous ethanol into the same test tube, mixing, standing at room temperature in dark for 30min, measuring absorbance at 517nm, and recording as A₂Each group was run in triplicate. The DPPH radical clearance was calculated according to the following formula:

DPPH radical scavenging ratio (%) - (A)₀-(A₁-A₂))/A₀×100％

The results are shown in table 3 below.

TABLE 3 product free radical scavenging capacity containing plant exosomes and resveratrol

As can be seen from Table 3, DPPH radical clearance can be very high with the product containing plant exosomes and resveratrol provided by the present invention. Through screening the types of the components in the product containing the plant exosomes and the resveratrol or adjusting the weight parts of the components in the product containing the plant exosomes and the resveratrol, the DPPH free radical clearance can be obviously improved and can reach about 79.1 percent, so that the product containing the plant exosomes and the resveratrol has obvious free radical eliminating capacity. And the DPPH free radical clearance is 50% or below after the product which replaces the components of the product containing the plant exosomes and the resveratrol and adjusts the weight parts of the components in the product containing the plant exosomes and the resveratrol is used, which indicates that the product containing the plant exosomes and the resveratrol, which is obtained by replacing the components of the product containing the plant exosomes and the resveratrol or adjusting the weight parts of the product containing the plant exosomes and the resveratrol, has unsatisfactory free radical elimination capability.

Experiment 3: verification of antioxidant and anti-aging activity

And (3) testing a sample: examples 1-3 samples prepared

The experimental method comprises the following steps:

cell culture and treatment:

3000 human epidermal fibroblasts (HFB) (P3-P7) were inoculated into each well of a 96-well plate, cultured in an incubator for 24 hours (5% CO2, 37 ℃), and then replaced with a drink of example 1-3 containing vitamin C (positive control) at a final concentration of 10. mu.g/m L after 24 hours, and H was continuously added after 1 hour of treatment₂O₂The solution was brought to a final concentration of 800. mu. mol/L, followed by incubation for 72 h.

The CCK8 method measures cell proliferation activity:

after 72 hours of incubation, the culture broth was aspirated and washed once with PBS buffer, followed by addition of culture broth containing 10% CCK-8 and incubation in a cell incubator for 2 hours in the absence of light. Then, the OD value was measured at a wavelength of 450nm using a microplate reader, and the cell proliferation activity was calculated by the following formula:

percent (%) cell proliferation activity (%) as OD test/OD control × 100%

The results are shown in Table 4 below.

TABLE 4 antioxidant and antiaging activity of product containing plant exosomes and resveratrol

Group of	Percentage of cell proliferation Activity (%)
		Blank group	100±4.2
H₂O₂Group of	44.9±4.6
		H₂O₂+ vitamin C group	53.4±5.0
H₂O₂+ EXAMPLE 1 group	59.8±5.7
		H₂O₂+ EXAMPLE 2 group	67.7±4.3
H₂O₂+ EXAMPLE 3 group	79.7±6.2

As can be seen from Table 4, for the use H₂O₂After the same concentration of vitamin C and the products containing the plant exosomes and the resveratrol in the examples 1, 2 and 3 are added into the HFB cells treated by the solution, the cell proliferation activity can be enhanced, and the oxidative damage of the cells caused by the hydrogen peroxide can be inhibited. Percentage of cell proliferation activity: h₂O₂+ example 1 group H₂O₂+ example 2 group H₂O₂+ examples 3 groups all are greater than H₂O₂+ vitamin C group, and H₂O₂+ example 3 group max. It is demonstrated that the products containing plant exosomes and resveratrol according to the examples of the invention have superior activity ability to proliferate oxidatively damaged cells compared to vitamin C.

Experiment 4: stability verification

And (3) testing a sample: examples 1-3, comparative examples 1-12

The experimental method comprises the following steps:

the test samples are tested for 1 month, 3 months, 6 months, 12 months and 18 months under four test conditions of-18 ℃, room temperature, 45 ℃ and-18-45 ℃ alternately, and the test results are shown in the following table 5 after the test samples are observed to have obvious change, pass change and fail change.

Table 5 stability test results

From the above results, it can be seen that:

after the product containing the plant exosomes and the resveratrol is tested for 1 month, 3 months, 6 months, 12 months and 18 months under four test conditions of-18 ℃, room temperature, 45 ℃ and-18-45 ℃ alternately, the test results are all qualified, namely the product containing the plant exosomes and the resveratrol has good cold resistance and heat resistance, and can meet the use requirements under the condition of extreme temperature change. The antioxidant drink without the formula system has poor cold resistance and heat resistance under long-term storage conditions, and cannot meet the use requirements under extreme temperature change conditions.

It is to be understood that the invention disclosed is not limited to the particular methodology, protocols, and materials described, as these may vary. It is also to be understood that the terminology used herein is for the purpose of describing particular embodiments only, and is not intended to limit the scope of the present invention which will be limited only by the appended claims.

Those skilled in the art will also recognize, or be able to ascertain using no more than routine experimentation, many equivalents to the specific embodiments of the invention described herein. Such equivalents are intended to be encompassed by the following claims.

Claims

1. A product comprising plant exosomes and resveratrol, the product comprising plant exosomes comprising red grape exosomes, red pomegranate exosomes and carrot exosomes and resveratrol.

2. The product of claim 1, further comprising grape fruit powder, pomegranate concentrate powder, yeast β -glucan, yeast extract, cranberry concentrate, elderberry concentrate, astaxanthin, olive powder, momordica grosvenori concentrate, xanthan gum, potassium sorbate, and water.

3. The product according to claim 1, wherein the product comprises 0.5-10 parts of red grape exosomes, 0.5-8 parts of red pomegranate exosomes, 0.1-6 parts of carrot exosomes and 0.1-3 parts of resveratrol by weight.

4. The product of claim 2, wherein the product comprises, in parts by weight:

5. The product containing plant exosomes and resveratrol is prepared from the following components in parts by weight:

6. A product containing plant exosomes and resveratrol is prepared from the following components in parts by weight:

7. A product containing plant exosomes and resveratrol is prepared from the following components in parts by weight:

8. Use of red grape exosomes, red pomegranate exosomes, carrot exosomes and/or resveratrol or a composition comprising red grape exosomes, red pomegranate exosomes, carrot exosomes and resveratrol in the preparation of a product comprising plant exosomes and resveratrol.

9. The product according to any one of claims 1 to 7 or the use according to claim 8, wherein,

the red grape exosome is prepared by the following method:

10. The product according to any one of claims 1 to 7 or the use according to claim 8, wherein,

the pomegranate exosome is prepared by the following method:

11. The product according to any one of claims 1 to 7 or the use according to claim 8, wherein,

the carrot exosome is prepared by the following method:

12. A process for the preparation of a product containing plant exosomes and resveratrol, wherein the process comprises the steps of: