CN111436461A - Biological herbicide - Google Patents
Biological herbicide Download PDFInfo
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- CN111436461A CN111436461A CN202010218161.3A CN202010218161A CN111436461A CN 111436461 A CN111436461 A CN 111436461A CN 202010218161 A CN202010218161 A CN 202010218161A CN 111436461 A CN111436461 A CN 111436461A
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- Prior art keywords
- soot
- tea
- weeds
- bioherbicide
- spores
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
Abstract
The invention discloses a biological herbicide, which belongs to the technical field of herbicides and comprises bacterial powder and nutrient solution produced by artificially propagating tea coal disease pathogenic bacteria. The biological herbicide provided by the invention utilizes saprophytic pathogenic bacteria-tea coal disease pathogenic bacteria as an active ingredient, has only nutritional requirements, has no parasitic selectivity, completely covers all weeds in a tea garden by a weeding spectrum, provides a proper growth environment for spores, enables the spores to proliferate on leaves of the weeds, and inhibits the growth of the weeds.
Description
Technical Field
The invention belongs to the technical field of herbicides, and particularly relates to a biological herbicide.
Background
Weeds are always a difficult problem for growers in the plant growing process. Weeds compete with crops for growth space, not only influence the growth of the crops and hinder the farming activities, but also induce diseases and insect pests. Weeding is an important farming activity. Although the common chemical herbicide is simple and efficient, the herbicide has chronic phytotoxicity on crops, easily causes pesticide residues and destroys the ecological balance of soil, and is forbidden in some tea areas particularly for special crops such as tea. At present, the tea garden is mainly used for hoeing artificially and mowing mechanically, and the problems of low working efficiency, labor consumption and high cost exist. The trend that chemical herbicides are forbidden in tea gardens is established, the greatest problem of pollution-free weed control in established tea areas is solved, and the development of a green, efficient, wide-adaptability and low-cost weeding technology is urgently needed. Biological weeding is a green weeding method, but at present, the active ingredients of the biological herbicide are pathogenic microorganisms of individual weeds, generally are obligate parasitic bacteria, have narrow weeding spectrum, are difficult to deal with complicated grass facies of tea gardens, and cannot be put into practical use.
The mould is full of living space of people, the saprophytic mould takes residual organic nutrient components as nutrient sources for proliferation, the trouble is brought to human life, and some moulds can cause diseases. The sooty mould is a disease on tea trees, wherein the saprophytic sooty mould is also a kind of mould, black small mould spots are formed on leaf surfaces and branch tips by the mould, and when the black mould layers are distributed on the whole leaf surfaces and the young tips in serious conditions, the photosynthesis of the leaves is influenced, the tree vigor is weakened, the saprophytic sooty mould is not in large relation with a specific host, and secretions and excretions of insects such as whitefly, aphids, leafhopper, scale insect and the like, even dust is mainly used as a nutrient source. The invention is intended for the biological control of weeds with moulds.
Disclosure of Invention
In order to overcome the defects of the prior art, the technical problems to be solved by the invention are as follows: provides a biological herbicide which can effectively kill weeds in a tea garden without causing pesticide residues.
In order to solve the technical problems, the invention adopts the technical scheme that: a biologic herbicide is prepared from the bacterial powder of the pathogenic bacteria of tea-coal disease through artificial reproduction and nutritive agent.
Wherein the pathogenic bacteria of the tea coal disease is one or more of special-enriched coal soot, tea hammer shell soot, field fresh soot, optical shell soot, head gel soot, java black soot and acantho trifurcata soot.
The invention has the beneficial effects that: the biological herbicide provided by the invention utilizes saprophytic pathogenic bacteria-tea coal disease pathogenic bacteria as an active ingredient, has only nutritional requirements, has no parasitic selectivity, completely covers all weeds in a tea garden by a weeding spectrum, provides a proper growth environment for spores, enables the spores to proliferate on leaves of the weeds, and inhibits the growth of the weeds.
Detailed Description
In order to explain the technical content, the objects and the effects of the present invention in detail, the following description will be given with reference to the embodiments.
The most key concept of the invention is as follows: the pathogenic bacteria of tea coal disease existing in the tea garden are used as the activity to prevent and control weeds.
The biological herbicide comprises bacterial powder and a nutrient which are produced by artificially propagating tea coal disease pathogenic bacteria.
From the above description, the beneficial effects of the present invention are: the biological herbicide provided by the invention utilizes saprophytic pathogenic bacteria-tea coal disease pathogenic bacteria as an active ingredient, has only nutritional requirements, has no parasitic selectivity, completely covers all weeds in a tea garden by a weeding spectrum, provides a proper growth environment for spores, enables the spores to proliferate on leaves of the weeds, and inhibits the growth of the weeds.
Further, the pathogenic bacteria of tea coal disease are one or more of soot rich (pharagmopapnia beta (syd.p.syd.ete.j.butler) theiss.et Syd.), soot shell (neocapnadium vulgaris (Hara), field new soot (neocapnadium tanakae (Shirai et Hara) w.yamam), soot shell (L imalia sp.), soot shell (scrolas catata Sawada), soot shell (L imalia japonica (Zimm.) Hohn), and soot triple plug (tripospodopsis spore graft (Hohn) w.yamam).
As can be seen from the above description, the above-mentioned strains have a high infection rate to the weeds in the garden and are effective in killing the weeds in the tea garden.
Further, the spore content of the bacterial powder is 100 × 108spores/g。
Further, the nutritional agent includes glycerol, sucrose, glucose, fructose, mannose, galactose, ammonium sulfate and water.
Further, each 75kg of water comprises 0.5-1.0kg of glycerol, 1.5-2kg of sucrose, 0.3-0.5kg of glucose, 0.5-1kg of fructose, 0.1-0.2kg of mannose, 0.1-0.2kg of galactose and 50-75g of ammonium sulfate.
Further, the use concentration of the bacterial powder is 1.0 × 109/ml-1.5×109spores/ml。
Further, the biological herbicide comprises 75kg of water, 0.5kg of glycerol, 1.5kg of sucrose, 0.5kg of glucose, 0.5kg of fructose, 0.1kg of mannose, 0.1kg of galactose, 50g of ammonium sulfate and special soot-rich bacteria powder, wherein the spore concentration of the special soot-rich bacteria in the biological herbicide is 1.2 × 109 spores/ml.
Example 1:
adding glycerol 0.5kg, sucrose 1.5kg, glucose 0.5kg, fructose 0.5kg, mannose 0.1kg, galactose 0.1kg, and ammonium sulfate 50g into 75kg of drinking water, stirring thoroughly to dissolve, adding artificially expanded soot-rich bacteria powder, stirring thoroughly to even, microscopic examination of soot-rich bacteria spore concentration 1.2 × 109Spores per ml to obtain the biological herbicide.
4, 10 days in 2019, the biological herbicide is sprayed in a Fuyun No. 6 variety 3-age young tea garden with the altitude of more than 600 meters in the tea area in east Fujian, and the biological herbicide is sprayed towards weeds, the weeds in the test tea garden comprise horseweed herb, bromelia, horehound, plantain, goosegrass, black nightshade, endive, mud weed, sticktight, bunge corydalis herb, speedwell, shepherd's purse, gynura divaricata and the like, the biological herbicide can be sprayed in one mu of tea garden, and after the biological herbicide is sprayed for 7 days, the average infection rate of the leaves of a weed population is found by statistics, the leaves of tea seedlings are not infected, and the coverage and the average height of the weed population are not changed after 14 days.
Example 2:
adding 1kg of glycerol, 1.6kg of sucrose, 0.3kg of glucose, 1kg of fructose, 0.15kg of mannose, 0.2kg of galactose and 75g of ammonium sulfate into 75kg of drinking water, fully stirring until dissolving, then adding artificially expanded soot powder of Aconitum pendulum shell, fully stirring uniformly, and examining the spore concentration of Aconitum pendulum shell soot 1.0 × 10 under a microscope9Spores per ml to obtain the biological herbicide.
4, 4 and 10 days in 2019, the biological herbicide is sprayed in a 3-age young tea garden of a Fuding white tea variety with the altitude of more than 600 meters in the east Fujian tea area, and the biological herbicide is sprayed towards weeds, weeds such as horseweed herb, creeping oxalis, fish eye grass, plantain herb, black nightshade, sambucus samarangensis, polygonum, endive, sowthistle herb, sticktight, bunge corydalis herb, speedwell, gynura divaricata and the like exist in the test tea garden, the biological herbicide can be sprayed in one mu of tea garden, the average infection rate of the leaves of a weed population is found by statistics after 7 days of spraying the biological herbicide, the leaves of tea seedlings are free of infection, and the population coverage and the average height of the weeds are not changed after 14 days.
Example 3:
adding glycerol 0.8kg, sucrose 2kg, glucose 0.4kg, fructose 0.6kg, mannose 0.2kg, galactose 0.15kg, and ammonium sulfate 60g into 75kg of drinking water, stirring thoroughly to dissolve, adding artificially expanded and produced soot powder, stirring thoroughly to even, microscopic examination of soot spore concentration of soot spore of soot shell of java 1.5 × 109Spores per ml to obtain a biological herbicide.
4, 15 days in 2019, the biological herbicide is sprayed in a 3-age young tea garden of a yellow kwan-yin variety with the altitude of 50 meters in a tea area in east Fujian, the biological herbicide is sprayed towards weeds, the weeds in the tea garden tested comprise weeds such as bromelia, creeping oxalis, horehound, eleusine indica, black nightshade, sambucus chinensis, endive, sticktight, bunge corydalis herb, buttercup, speedwell, gynura bicolor and the like, the biological herbicide can be sprayed in one mu of tea garden, and after 7 days of spraying of the biological herbicide, the average infection rate of the leaves of a weed group is statistically found to be 96%, the leaves of tea seedlings are not infected, and the coverage and the average height of the weed group are not changed after 14 days.
Example 4:
adding glycerol 0.5kg, sucrose 1.5kg, glucose 0.5kg, fructose 0.5kg, mannose 0.1kg, galactose 0.1kg, and ammonium sulfate 50g into 75kg of drinking water, stirring thoroughly to dissolve, adding artificially expanded soot-rich bacteria powder, stirring thoroughly to even, microscopic examination of soot-rich bacteria spore concentration 1.8 × 109Spores per ml to obtain a biological herbicide.
4, 4 and 15 days in 2019, the biological herbicide is sprayed in a tea garden of which the plum accounts for 2 years and the altitude is more than 100 meters in a tea area in east Fujian, the tea garden is sprayed towards weeds, the weeds in the tea garden tested comprise weeds such as erigeron breviscapus, bromus, creeping oxalis, ophthal, eleusine indica, sambucus chinensis, polygonum hydropiper, endive, mud weeds, bunge corydalis herb, speedwell, shepherd's purse and the like, the biological herbicide can be sprayed in one mu of the tea garden, and after 7 days of spraying the biological herbicide, the average infection rate of the leaves of a weed population is statistically found to be 95%, the leaves of tea seedlings are not infected, and the coverage and the average height of the weed population are not changed after 14 days.
Example 5:
adding glycerol 0.5kg, sucrose 1.5kg, glucose 0.5kg, fructose 0.5kg, mannose 0.1kg, galactose 0.1kg, and ammonium sulfate 50g into 75kg of drinking water, stirring thoroughly to dissolve, adding artificially expanded soot-rich bacteria powder, stirring thoroughly to even, microscopic examination of soot-rich bacteria spore concentration 0.8 × 109Spores per ml to obtain a biological herbicide.
In 2019, 4 and 20 days, the biological herbicide is sprayed in a 3-age young tea garden of a purple rose variety with the altitude of more than 300 meters in a tea area in east Fujian, the biological herbicide is sprayed towards weeds, the weeds in the tea garden tested comprise erigeron breviscapus, bromus, plantain, goosegrass, polygonum, endive, mud weed, bunge corydalis herb, buttercup, speedwell, gynura bicolor and the like, the biological herbicide can be sprayed to the tea garden for one mu, and after 7 days of spraying the biological herbicide, the average infection rate of the leaves of a weed population is found to be 75 percent, the infection rate is 85 percent after 14 days, the leaves of tea seedlings are not infected, and the coverage and the average height of the weed population are unchanged after 14 days.
In conclusion, the biological herbicide provided by the invention utilizes the saprophytic pathogenic bacteria-tea coal disease pathogenic bacteria as an active ingredient, has only nutritional requirements, has no parasitic selectivity, and completely covers all weeds in the tea garden by a weeding spectrum.
The above description is only an embodiment of the present invention, and not intended to limit the scope of the present invention, and all equivalent modifications made by the present invention in the specification or directly or indirectly applied to the related technical field are included in the scope of the present invention.
Claims (7)
1. A biological herbicide is characterized by comprising bacterial powder and nutrient solution which are produced by artificially propagating pathogenic bacteria of tea coal diseases.
2. The bioherbicide of claim 1 wherein said tea-coal pathogenic bacteria is one or more of special-rich soot, tea hammer shell soot, field fresh soot, optical shell soot, gelatin shell soot, java black soot, and stinging trigeminal soot.
3. The bioherbicide of claim 1 wherein said fungal powder has a spore content of 100 × 108spores/g。
4. A bioherbicide as claimed in claim 1 wherein said nutrient solution comprises glycerol, sucrose, glucose, fructose, mannose, galactose, ammonium sulphate and water.
5. A bioherbicide as claimed in claim 4, characterized in that it comprises 0.5-1.0kg of glycerol, 1.5-2kg of sucrose, 0.3-0.5kg of glucose, 0.5-1kg of fructose, 0.1-0.2kg of mannose, 0.1-0.2kg of galactose and 50-75g of ammonium sulphate per 75kg of water.
6. The bioherbicide of claim 1 wherein said fungal powder is used at a concentration of 1.0 × 109/ml-1.5×109spores/ml。
7. The bioherbicide of claim 1, which is characterized by consisting of 75kg of water, 0.5kg of glycerin, 1.5kg of sucrose, 0.5kg of glucose, 0.5kg of fructose, 0.1kg of mannose, 0.1kg of galactose, 50g of ammonium sulfate and soot-rich bacteria powder, wherein the spore concentration of soot-rich bacteria in the bioherbicide is 1.2 × 109Spores per ml.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010218161.3A CN111436461A (en) | 2020-03-25 | 2020-03-25 | Biological herbicide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010218161.3A CN111436461A (en) | 2020-03-25 | 2020-03-25 | Biological herbicide |
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| CN111436461A true CN111436461A (en) | 2020-07-24 |
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| CN202010218161.3A Pending CN111436461A (en) | 2020-03-25 | 2020-03-25 | Biological herbicide |
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Citations (10)
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| CN1994092A (en) * | 2006-12-26 | 2007-07-11 | 浙江省农业科学院 | A biological weed killer and preparation process thereof |
| CN101371666A (en) * | 2007-08-21 | 2009-02-25 | 董晔欣 | Application of Pestalotiopsis guepinii Stey. toxin in herbicide |
| CN101736032A (en) * | 2009-03-13 | 2010-06-16 | 南京信息工程大学 | Method for culturing toxin by using fusarium oxysporum and use of toxin as herbicide |
| CN101919412A (en) * | 2010-07-20 | 2010-12-22 | 浙江省农业科学院 | Colletotrichum Corda strain and biological herbicide containing spores thereof |
| US20120059038A1 (en) * | 2009-05-14 | 2012-03-08 | Astellas Pharma Inc. | Polyketide compound |
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2020
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Patent Citations (10)
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| CN1165536A (en) * | 1995-09-18 | 1997-11-19 | 日本烟草产业株式会社 | Microorganism belonging to the genus ascochyta and use of the same |
| CN1994092A (en) * | 2006-12-26 | 2007-07-11 | 浙江省农业科学院 | A biological weed killer and preparation process thereof |
| CN101371666A (en) * | 2007-08-21 | 2009-02-25 | 董晔欣 | Application of Pestalotiopsis guepinii Stey. toxin in herbicide |
| CN101736032A (en) * | 2009-03-13 | 2010-06-16 | 南京信息工程大学 | Method for culturing toxin by using fusarium oxysporum and use of toxin as herbicide |
| US20120059038A1 (en) * | 2009-05-14 | 2012-03-08 | Astellas Pharma Inc. | Polyketide compound |
| CN101919412A (en) * | 2010-07-20 | 2010-12-22 | 浙江省农业科学院 | Colletotrichum Corda strain and biological herbicide containing spores thereof |
| CN102517245A (en) * | 2011-12-16 | 2012-06-27 | 北京丹路实业公司 | Liquid production technology for weed bio-control product |
| CN103814953A (en) * | 2014-01-11 | 2014-05-28 | 哈尔滨工业大学宜兴环保研究院 | Fungal field weed inhibitor and preparation method thereof |
| WO2018156457A1 (en) * | 2017-02-24 | 2018-08-30 | Paramount Products 1 Llc | Treatment for plants in conjunction with harvesting |
| CN109122727A (en) * | 2018-07-16 | 2019-01-04 | 华南农业大学 | Application of colletotrichum gloeosporioides BWH-1 in preparation of herbicide |
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