CN102517245A - Liquid production technology for weed bio-control product - Google Patents
Liquid production technology for weed bio-control product Download PDFInfo
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- CN102517245A CN102517245A CN2011104234372A CN201110423437A CN102517245A CN 102517245 A CN102517245 A CN 102517245A CN 2011104234372 A CN2011104234372 A CN 2011104234372A CN 201110423437 A CN201110423437 A CN 201110423437A CN 102517245 A CN102517245 A CN 102517245A
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- conidia
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Abstract
The invention discloses a liquid production technology for a weed bio-control product, and belongs to the technical field of biological engineering. Cuscutaaustralis (C.chinensis; C.maritima) can be effectively prevented and removed by using a Lubao No.1 strain [Colletotricumgloeosporioides (Penz.) Sacc. F. sp. cuscutae]. The conidia solid bactericide has a remarkable prevention and control effect, but the production efficiency is low and the product quality is difficult to guarantee. The technology successfully solves the technical method of liquid fermentation. In the technical path, a group of liquid medium and culture conditions thereof suitable for propagation of conidia are determined by screening. The forming proportion of the conidia reaches over 95 percent, and the number of the conidia reaches 1 billion per milliliter. The technology can be used for short-time large-scale industrialized production, the availability rate of the conidia is higher than that of a solid fermented product, and a reliable administration means is provided for preventing and controlling large-area outburst cuscuta chinensis disaster.
Description
Technical field
" a kind of liquid prodn technology of weeds biological and ecological methods to prevent plant disease, pests, and erosion goods " belong to technical field of bioengineering.Relate to biological microbiology, molecular biology, biological chemistry and cytology, relate to modern project technology such as phytology, chemical industry, machinery.
Background technology
South Dodder Seed Chinese Dodder Seed is the parasitics weeds of a kind of mainly cause harm pulse family, plant of Solanaceae, and parasitic winding of its filament descends the crop failure quality, and is heavy then have no harvest.Receive the farm crop of its harm that oil plant, vegetables, Chinese medicinal materials etc. are arranged.
Utilize the Shandong to protect the conidium goods that a bacterium [colletotrichum gloeosporioides Penz South Dodder Seed Chinese Dodder Seed specialized form bacterial strain Colletotricum gloeosporioides (Penz.) Sacc.f.sp.cuscutae] produces, can effectively prevent and kill off parasitics weeds South Dodder Seed Chinese Dodder Seed (Cuscuta australis; C.chinensis; C.maritima).The production of relevant product mainly is to adopt effective strain, produces through solid culture when the conidium solid fungicide that contains some amount supplies the South Dodder Seed Chinese Dodder Seed control as product (commodity) and uses.Method of use is converted the water logging bubble with solid fungicide and is got its clear liquid sprinkling.Control effect is remarkable, and is pollution-free.Weeds biological and ecological methods to prevent plant disease, pests, and erosion product and Prevention Technique thereof that this is developed voluntarily and applied by China have become and have utilized fungi to prevent and kill off parasitics weeds successful example in the world.It is comparatively simple that solid fermentation is produced desired equipment, and production technology is easy to grasp, but production efficiency is low, and quality product is difficult to guarantee that can not produce typical products in mass production at short notice is that sudden big area South Dodder Seed Chinese Dodder Seed disaster provides the control medication.
Utilize biotechnology liquid fermenting production technology, manufacture plant pathogenic fungi and the effective verivate of mikrobe thereof and carry out the weeds biological control.Be that liquid fermenting can address the above problem.Summer in 2006,3000 mu of Radix Astragali burst South Dodder Seed Chinese Dodder Seed disasters of the regional all over the city medicinal herb grower's plantation in Hebei, the medicinal herb grower watches that crop faces total crop failure.Agricultural sector of local government finds Dan Lu company to require to solve harm.Receive liquid bacterial agent of Shandong guarantor that task Dan Lu company makes with present technique, send in 3 days in disaster-stricken medicinal herb grower's hand, result of use is remarkable, has prevented the condition of a disaster to keep Radix Astragali output.Receiving the medicinal herb grower praises highly.15 yuan/mu of drug costs.
Research subject matter about liquid fermenting is at present, and mostly thalline is mycelium in the fermented liquid, and conidium seldom utilizes a large amount of conidiums of the very difficult formation of liquid culture not have use value.
Summary of the invention
The present invention is according to the biological characteristics of this bacterium, and the condition that conidium forms is through the technological method of a large amount of Success in Experiment solution liquid fermentings.One group of liquid nutrient medium and culture condition thereof that suitable conidium breeding generates that technology path, screening are confirmed.Can make that conidium formation ratio reaches more than 95% in the nutrient solution, 1,000,000,000/milliliter of conidium up.Present technique adopts fermentor tank to carry out the industriallization liquid submerged fermentation, can large-scale industrial production, can the short period of time produce bacterium liquid product in enormous quantities, and the conidium availability of output is higher than conventional solid fermentation product.Present technique has improved quality product, has reduced cost.The bacterium liquid that present technique is produced dilutes in proportion to be converted water and sprays and can kill South Dodder Seed Chinese Dodder Seed rapidly, and easy to use, labour intensity is low.For in time preventing and treating big area burst South Dodder Seed Chinese Dodder Seed disaster reliable medication means are provided.
Embodiment
1. substratum.Medium component and ratio thereof under the liquid culture condition.
Crude dextrose: Semen Maydis powder: soybean cake powder: wheat bran: water 1:1:0.5:0.5:97
2. culture condition.Fermentor cultivation.
Sterilization: 121 ℃ of 30 minutes time length in the substratum tank body.
Inoculation: aseptic inoculation prevents to dye assorted bacterium.
PH value: the initial pH 6.0～7.5 of substratum
Air flow: aseptic air flow 0.8～1.0v/v/min
Tank pressure: 0.05Mpa
Temperature: interior 30 ± 2 ℃ of jar
Rotating speed: mixing speed 200r/min
Time: fermentation time 52 hours
3. finished product standard.
Color: fermented liquid orange
Rate of formation: conidium＞95%
Quantity: conidium number>=10
9/ ml (1,000,000,000/milliliter)
Packing: impurity screening, can
Storage: room temperature storage below 25 ℃
Quality guaranteed period: 60 days
Method of use: bacterium liquid is converted water and is sprayed, ratio, bacterium liquid: water 1:50.
Claims (2)
1. medium component and ratio thereof under the liquid culture condition, crude dextrose: Semen Maydis powder: soybean cake powder: water, 1:1:0.5:97.5.
2. the culture condition fermentor cultivation is sterilized, 121 ℃ of 30 minutes time length in the substratum tank body; Inoculation, aseptic inoculation prevent to dye assorted bacterium; The pH value, the initial pH 6.0～7.5 of substratum; Air flow, aseptic air flow 0.8～1.0v/v/min; Tank pressure, 0.05mpa; Temperature, interior 30 ± 2 ℃ of jar; Rotating speed, mixing speed 200r/min; Time, fermentation time 52 hours.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011104234372A CN102517245A (en) | 2011-12-16 | 2011-12-16 | Liquid production technology for weed bio-control product |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011104234372A CN102517245A (en) | 2011-12-16 | 2011-12-16 | Liquid production technology for weed bio-control product |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102517245A true CN102517245A (en) | 2012-06-27 |
Family
ID=46288315
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011104234372A Pending CN102517245A (en) | 2011-12-16 | 2011-12-16 | Liquid production technology for weed bio-control product |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102517245A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109122727A (en) * | 2018-07-16 | 2019-01-04 | 华南农业大学 | Application of colletotrichum gloeosporioides BWH-1 in preparation of herbicide |
| CN111436461A (en) * | 2020-03-25 | 2020-07-24 | 福建省农业科学院茶叶研究所 | Biological herbicide |
-
2011
- 2011-12-16 CN CN2011104234372A patent/CN102517245A/en active Pending
Non-Patent Citations (2)
| Title |
|---|
| 朱秦: "胶孢炭疽菌作为防除波斯婆婆纳的生物除草剂产业化相关技术的研究", 《中国优秀硕士学位论文全文数据库工程科学I辑》 * |
| 谷峻等: "生物除草剂菟丝子胶孢炭疽菌生物学特性的研究", 《江苏农业科学》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109122727A (en) * | 2018-07-16 | 2019-01-04 | 华南农业大学 | Application of colletotrichum gloeosporioides BWH-1 in preparation of herbicide |
| CN109122727B (en) * | 2018-07-16 | 2021-02-19 | 华南农业大学 | Application of colletotrichum gloeosporioides BWH-1 in preparation of herbicide |
| CN111436461A (en) * | 2020-03-25 | 2020-07-24 | 福建省农业科学院茶叶研究所 | Biological herbicide |
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Application publication date: 20120627 |