CN110683894A - Active cell microalgae repairing liquid and preparation method thereof - Google Patents
Active cell microalgae repairing liquid and preparation method thereof Download PDFInfo
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- CN110683894A CN110683894A CN201910957505.XA CN201910957505A CN110683894A CN 110683894 A CN110683894 A CN 110683894A CN 201910957505 A CN201910957505 A CN 201910957505A CN 110683894 A CN110683894 A CN 110683894A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
- C05D9/02—Other inorganic fertilisers containing trace elements
Abstract
The invention discloses an active cell microalgae repairing liquid, which particularly relates to the technical field of agriculture, and comprises a plurality of microalgae in a living state, wherein the added microalgae comprise 10-50 parts of Haematococcus, 5-40 parts of blue algae, 10-30 parts of Phlomis verticillata and 30-50 parts of Goldfish algae according to the individual number of active cells of the microalgae, and the added microalgae comprise the following components in parts by weight; the invention also discloses a preparation method of the microalgae repairing liquid, which comprises the following operation steps: the method comprises the following steps: respectively culturing red hair algae, blue algae, stonewort and goldfish algae to obtain four kinds of microalgae solutions for later use. According to the invention, the plant growth regulator, the algae extract and the selenium, zinc and iron element extracts are added into the microalgae liquid, so that the fertilizer efficiency of the soil can be improved, the soil has the effects of killing insects and bacteria, killing bacteria in the soil and on the surface of the soil, improving the decomposition capability of the soil and decomposing organic matters, and the soil remediation efficiency is high.
Description
Technical Field
The invention relates to the technical field of agriculture, in particular to an active cell microalgae repairing liquid and a preparation method thereof.
Background
When the soil fertility level can not reach the nutrients required by plant growth, the soil needs to be fertilized, chemical fertilizer is widely used at present, but the negative effects brought by the application of the chemical fertilizer cannot be ignored: the raw materials for producing the chemical fertilizer contain heavy metals such as Zn, Cu, Co, Cr and the like, and the heavy metals are applied to the soil along with the chemical fertilizer, so that the heavy metal elements in the soil are enriched and enter the plant body through the absorption of the plant, and the growth of the plant and the quality of agricultural products and the like are influenced; the chemical fertilizer can reduce the quantity and activity of soil microorganisms, thereby reducing the conversion of organic matters in soil by the soil microorganisms, the decomposition of minerals and the degradation of toxic substances; the long-term application of chemical fertilizers can aggravate the acidification of soil, aggravate the hardening of the soil, inhibit the growth of plant roots and seriously reduce the stress resistance of plants.
Microalgae are autotrophic plants which are widely distributed in land soil, rivers and lakes and have high photosynthetic availability, are micro algae groups of which the forms can be distinguished under a microscope, are rich in nutrition, can generate nutrient substances such as polysaccharide and protein through cell metabolism, and have high application value in the fields of food, medicine, feed and the like.
However, the existing remediation liquid cannot kill bacteria in the soil and on the surface of the soil, and the soil remediation rate is low.
Disclosure of Invention
In order to overcome the above-mentioned drawbacks of the prior art, embodiments of the present invention provide an active cell microalgae repairing liquid and a preparation method thereof, in which a plant growth regulator, an algae extract, and selenium, zinc, and iron element extracts are added to a microalgae liquid, so that not only can the fertilizer efficiency of soil be improved, but also the microalgae repairing liquid has insecticidal and bactericidal effects, can kill bacteria in and on the surface of the soil, improve the decomposition capability of the soil, and decompose organic matters, so as to improve the soil repairing efficiency, thereby solving the problems in the background art.
In order to achieve the purpose, the invention provides the following technical scheme: the active cell microalgae repairing liquid comprises a plurality of microalgae in a living state, wherein the added microalgae comprise 10-50 parts of hirsutella, 5-40 parts of blue algae, 10-30 parts of stonewort and 30-50 parts of goldfish algae by mass of the individual number of active cells of the microalgae, and 5 parts of plant growth regulator is also added.
In a preferred embodiment, the added microalgae comprise 5-35 parts of red hair algae, 10-30 parts of blue algae, 10-25 parts of stonewort and 35-50 parts of goldfish algae in terms of the number of the individual microalgae active cells.
In a preferred embodiment, the plant growth regulator in the raw material for preparation comprises three types of plant growth regulators for increasing amino acid content, increasing protein content and increasing stress resistance, and the plant growth regulator for increasing amino acid content comprises: plant growth regulator for increasing protein content: stress-resistance-improving plant growth regulators =1: 2.
in a preferred embodiment, the amino acid content-increasing plant growth regulator is formed by mixing paclobutrazol, atrazine and indole mature ester, the protein content-increasing plant growth regulator is formed by mixing atrazine, simazine, atrazine and naphthylacetic acid, and the stress resistance-increasing plant growth regulator is formed by mixing abscisic acid, paclobutrazol, damycin and chlormequat chloride.
In a preferred embodiment, the raw materials for preparing the repairing liquid further include an algae extract and selenium, zinc and iron extracts, wherein the algae raw materials in the algae extract are the bifidobacterium unicum, the chlorella and the anabaena, and the parent raw materials of the selenium, zinc and iron extracts are the oyster mushroom, the oyster meat and the sedum.
The invention also provides a preparation method of the active cell microalgae repairing liquid, which comprises the following operation steps:
the method comprises the following steps: respectively carrying out expanded cultivation on the Rhodophyta, the blue algae, the stonewort and the Goldfish algae to obtain four microalgae solutions for later use;
step two: preparing three types of plant growth regulators capable of improving the amino acid content, the protein content and the stress resistance for later use;
step three: preparing algae extract and selenium, zinc and iron element extract for later use;
step four: and (3) fully mixing the four microalgae solutions in the steps with a plant growth regulator, the algae extract and the selenium, zinc and iron element extracts in sequence to obtain the active cell microalgae repairing solution.
In a preferred embodiment, the medium solution for expanding cultivation in the first step comprises the following components in concentration: 2-6mg/L disodium ethylene diamine tetraacetate, 5-13mg/L citric acid and 0.05-0.15mg/L FeSO4•7H2O、0.15-0.35g/L NaHCO3、0.03-0.1g/L CaCl2、0.05-0.2g/L MgSO4•7H2O、1-3.5g/L NaNO3、0.05-0.15g/L K2HPO4、0.2-1g/L KH2PO4、1.5-2.5mg/L MnCl2•4H2O、0.02-0.05mg/L ZnSO4•7H2O、0.05-0.1mg/L CuSO4•5H2O、0.02-0.05mg/L Na2MoO4•2H2O、2-4mg/L H3BO3。
In a preferred embodiment, the expanding cultivation process in the first step comprises: selecting and separating single microalgae cells, and carrying out hierarchical expansion cultivation on the single microalgae cells, wherein in each stage of expansion cultivation process, when the number of the microalgae cells is more than or equal to 106/mL, the next stage of expansion cultivation process is carried out, namely the single microalgae cells are cultivated in 15mL of culture medium solution for 10-15 days, 15mL of algae solution → transfer inoculation is carried out in 85mL of culture medium solution for continuous cultivation for 10-15 days, 100mL of algae solution is obtained → transfer inoculation is carried out in 0.9L of culture medium solution for continuous cultivation for 10-15 days, 1L of algae solution → transfer inoculation is carried out in 4L of culture medium solution for continuous cultivation for 10-15 days, 5L of algae solution is obtained → transfer inoculation is carried out in 13L of culture medium solution for continuous cultivation for 10-15 days, 18L of algae solution → transfer inoculation is carried out in 782L of culture medium solution for continuous cultivation for 10-15 days, and 800L of algae solution is obtained.
In a preferred embodiment, the extraction process of the algae extract in the third step is as follows: centrifuging and collecting algae cells of Bifidobacterium minutissima, Chlorella vulgaris and Anabaena for 3 times, continuously washing with distilled water, lyophilizing at-50 deg.C, storing for use, weighing appropriate amount of lyophilized powder of algae of three kinds, and adding appropriate amount of PBS buffer solution with pH of 7.0 to obtain algae extract with density of 1.0 g/L.
In a preferred embodiment, the extraction process of the selenium, zinc and iron element extract in the third step is that oyster mushroom, oyster meat and sedum are respectively mashed, centrifuged and filtered, and the obtained filtrate is the corresponding extract.
The invention has the technical effects and advantages that:
compared with the existing microalgae repairing liquid, the microalgae active cell nutrient repairing liquid has the advantages that the plant growth regulator, the algae extract and the selenium, zinc and iron element extracts are added, so that the fertilizer efficiency of soil can be improved during actual use, the insecticidal and bactericidal effects are achieved, bacteria in and on the surface of the soil can be killed, the decomposition capability of the soil is improved, organic matters are decomposed, the soil repairing efficiency is high, the growth environment of crops is improved, and the yield is increased.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The invention provides an active cell microalgae repairing liquid which comprises a plurality of microalgae in a living state, wherein the added microalgae comprises 10-50 parts of hirsutella, 5-40 parts of blue algae, 10-30 parts of verticillium, 8-10 parts of rice hull extract, 5-8 parts of sweet potato extract, 6-9 parts of mixed bacteria extract and 30-50 parts of hornworts according to the individual number of active cells of the microalgae, and 5 parts of plant growth regulator is also added according to the parts by weight.
Example 1
Preferably, in the preparation raw materials, the added microalgae comprise 5 parts of hirsutella, 10 parts of blue algae, 10 parts of stonewort, 35 parts of goldfish algae, 8 parts of rice hull extract, 5 parts of sweet potato extract and 6 parts of mixed bacteria extract according to the individual number of the microalgae active cells;
the plant growth regulator in the raw materials comprises three types of plant growth regulators for improving amino acid content, protein content and stress resistance, and the plant growth regulator for improving the amino acid content comprises the following components: plant growth regulator for increasing protein content: stress-resistance-improving plant growth regulators =1: 2;
the plant growth regulator for improving the amino acid content is formed by mixing paclobutrazol, atrazine and indole mature ester, the plant growth regulator for improving the protein content is formed by mixing atrazine, simazine, atrazine and naphthylacetic acid, and the plant growth regulator for improving the stress resistance is formed by mixing abscisic acid, paclobutrazol, daminozide and chlormequat chloride;
the preparation raw materials of the repairing liquid also comprise algae extracts and selenium, zinc and iron element extracts, wherein the algae raw materials in the algae extracts are the bifidobacterium brevicornum, the chlorella vulgaris and the anabaena, and the parent raw materials of the selenium, zinc and iron element extracts are oyster mushroom, oyster meat and sedum.
The invention also provides a preparation method of the active cell microalgae repairing liquid, which comprises the following operation steps:
the method comprises the following steps: respectively carrying out expanded cultivation on the Rhodophyta, the blue algae, the stonewort and the Goldfish algae to obtain four microalgae solutions for later use;
step two: preparing three types of plant growth regulators capable of improving the amino acid content, the protein content and the stress resistance for later use;
step three: preparing algae extract and selenium, zinc and iron element extract for later use;
step four: fully mixing the four microalgae solutions in the above steps with a plant growth regulator, an algae extract and selenium, zinc and iron element extracts in sequence to obtain an active cell microalgae repairing solution;
the culture medium solution for expanding culture in the first step comprises the following components in concentration: 2-6mg/L disodium ethylene diamine tetraacetate, 5-13mg/L citric acid and 0.05-0.15mg/L FeSO4•7H2O、0.15-0.35g/L NaHCO3、0.03-0.1g/LCaCl2、0.05-0.2g/L MgSO4•7H2O、1-3.5g/L NaNO3、0.05-0.15g/L K2HPO4、0.2-1g/L KH2PO4、1.5-2.5mg/L MnCl2•4H2O、0.02-0.05mg/L ZnSO4•7H2O、0.05-0.1mg/L CuSO4•5H2O、0.02-0.05mg/L Na2MoO4•2H2O、2-4mg/L H3BO3；
The expanding cultivation process in the first step comprises the following steps: selecting and separating a single microalgae cell, and performing stage expansion cultivation on the single microalgae cell, wherein in each stage of expansion cultivation process, when the number of the microalgae cells is more than or equal to 106/mL, the next stage of expansion cultivation process is performed, namely the single microalgae cell is cultivated in 15mL of culture medium solution for 10-15 days, 15mL of algae solution → transfer inoculation is performed to 85mL of culture medium solution for continuous cultivation for 10-15 days, 100mL of algae solution → transfer inoculation is performed to 0.9L of culture medium solution for continuous cultivation for 10-15 days, 1L of algae solution → transfer inoculation is performed to 4L of culture medium solution for continuous cultivation for 10-15 days, 5L of algae solution → transfer inoculation is performed to 13L of culture medium solution for continuous cultivation for 10-15 days, 18L of algae solution → transfer inoculation is performed to 782L of culture medium solution for continuous cultivation for 10-15 days, and 800L of algae solution is obtained;
the extraction process of the algae extract in the third step comprises the following steps: centrifuging and collecting algae cells of Bifidobacterium minutissima, Chlorella vulgaris and anabaena respectively, continuously washing with distilled water for 3 times, freeze-drying at-50 deg.C, storing for use, weighing appropriate amount of freeze-dried powder of algae of three kinds, and adding appropriate amount of PBS buffer solution with pH of 7.0 to obtain algae extract with density of 1.0 g/L;
the extraction process of the selenium, zinc and iron element extracts in the three steps comprises the steps of respectively mashing oyster mushroom, oyster meat and sedum aizoon, centrifuging and filtering to obtain filtrate, namely the corresponding extracts.
Example 2
Preferably, in the preparation raw materials, the added microalgae comprise 20 parts of rambutan, 25 parts of blue algae, 20 parts of stonewort, 40 parts of goldfish algae, 10 parts of rice hull extract, 8 parts of sweet potato extract and 9 parts of mixed bacteria extract according to the individual number of the microalgae active cells;
the plant growth regulator in the raw materials comprises three types of plant growth regulators for improving amino acid content, protein content and stress resistance, and the plant growth regulator for improving the amino acid content comprises the following components: plant growth regulator for increasing protein content: stress-resistance-improving plant growth regulators =1: 2;
the plant growth regulator for improving the amino acid content is formed by mixing paclobutrazol, atrazine and indole mature ester, the plant growth regulator for improving the protein content is formed by mixing atrazine, simazine, atrazine and naphthylacetic acid, and the plant growth regulator for improving the stress resistance is formed by mixing abscisic acid, paclobutrazol, daminozide and chlormequat chloride;
the preparation raw materials of the repairing liquid also comprise algae extracts and selenium, zinc and iron element extracts, wherein the algae raw materials in the algae extracts are the bifidobacterium brevicornum, the chlorella vulgaris and the anabaena, and the parent raw materials of the selenium, zinc and iron element extracts are oyster mushroom, oyster meat and sedum.
The invention also provides a preparation method of the active cell microalgae repairing liquid, which comprises the following operation steps:
the method comprises the following steps: respectively carrying out expanded cultivation on the Rhodophyta, the blue algae, the stonewort and the Goldfish algae to obtain four microalgae solutions for later use;
step two: preparing three types of plant growth regulators capable of improving the amino acid content, the protein content and the stress resistance for later use;
step three: preparing algae extract and selenium, zinc and iron element extract for later use;
step four: fully mixing the four microalgae solutions in the above steps with a plant growth regulator, an algae extract and selenium, zinc and iron element extracts in sequence to obtain an active cell microalgae repairing solution;
the culture medium solution for expanding culture in the first step comprises the following components in concentration: 2-6mg/L disodium ethylene diamine tetraacetate, 5-13mg/L citric acid and 0.05-0.15mg/L FeSO4•7H2O、0.15-0.35g/L NaHCO3、0.03-0.1g/LCaCl2、0.05-0.2g/L MgSO4•7H2O、1-3.5g/L NaNO3、0.05-0.15g/L K2HPO4、0.2-1g/L KH2PO4、1.5-2.5mg/L MnCl2•4H2O、0.02-0.05mg/L ZnSO4•7H2O、0.05-0.1mg/L CuSO4•5H2O、0.02-0.05mg/L Na2MoO4•2H2O、2-4mg/L H3BO3；
The expanding cultivation process in the first step comprises the following steps: selecting and separating a single microalgae cell, and performing stage expansion cultivation on the single microalgae cell, wherein in each stage of expansion cultivation process, when the number of the microalgae cells is more than or equal to 106/mL, the next stage of expansion cultivation process is performed, namely the single microalgae cell is cultivated in 15mL of culture medium solution for 10-15 days, 15mL of algae solution → transfer inoculation is performed to 85mL of culture medium solution for continuous cultivation for 10-15 days, 100mL of algae solution → transfer inoculation is performed to 0.9L of culture medium solution for continuous cultivation for 10-15 days, 1L of algae solution → transfer inoculation is performed to 4L of culture medium solution for continuous cultivation for 10-15 days, 5L of algae solution → transfer inoculation is performed to 13L of culture medium solution for continuous cultivation for 10-15 days, 18L of algae solution → transfer inoculation is performed to 782L of culture medium solution for continuous cultivation for 10-15 days, and 800L of algae solution is obtained;
the extraction process of the algae extract in the third step comprises the following steps: centrifuging and collecting algae cells of Bifidobacterium minutissima, Chlorella vulgaris and anabaena respectively, continuously washing with distilled water for 3 times, freeze-drying at-50 deg.C, storing for use, weighing appropriate amount of freeze-dried powder of algae of three kinds, and adding appropriate amount of PBS buffer solution with pH of 7.0 to obtain algae extract with density of 1.0 g/L;
the extraction process of the selenium, zinc and iron element extracts in the three steps comprises the steps of respectively mashing oyster mushroom, oyster meat and sedum aizoon, centrifuging and filtering to obtain filtrate, namely the corresponding extracts.
Example 3
Preferably, in the preparation raw materials, the added microalgae comprise 35 parts of rambutan, 30 parts of blue algae, 25 parts of stonewort, 50 parts of goldfish algae, 9 parts of rice hull extract, 7 parts of sweet potato extract and 8 parts of mixed bacteria extract according to the individual number of the microalgae active cells;
the plant growth regulator in the raw materials comprises three types of plant growth regulators for improving amino acid content, protein content and stress resistance, and the plant growth regulator for improving the amino acid content comprises the following components: plant growth regulator for increasing protein content: stress-resistance-improving plant growth regulators =1: 2;
the plant growth regulator for improving the amino acid content is formed by mixing paclobutrazol, atrazine and indole mature ester, the plant growth regulator for improving the protein content is formed by mixing atrazine, simazine, atrazine and naphthylacetic acid, and the plant growth regulator for improving the stress resistance is formed by mixing abscisic acid, paclobutrazol, daminozide and chlormequat chloride;
the preparation raw materials of the repairing liquid also comprise algae extracts and selenium, zinc and iron element extracts, wherein the algae raw materials in the algae extracts are the bifidobacterium brevicornum, the chlorella vulgaris and the anabaena, and the parent raw materials of the selenium, zinc and iron element extracts are oyster mushroom, oyster meat and sedum.
The invention also provides a preparation method of the active cell microalgae repairing liquid, which comprises the following operation steps:
the method comprises the following steps: respectively carrying out expanded cultivation on the Rhodophyta, the blue algae, the stonewort and the Goldfish algae to obtain four microalgae solutions for later use;
step two: preparing three types of plant growth regulators capable of improving the amino acid content, the protein content and the stress resistance for later use;
step three: preparing algae extract and selenium, zinc and iron element extract for later use;
step four: fully mixing the four microalgae solutions in the above steps with a plant growth regulator, an algae extract and selenium, zinc and iron element extracts in sequence to obtain an active cell microalgae repairing solution;
the culture medium solution for expanding culture in the first step comprises the following components in concentration: 2-6mg/L disodium ethylene diamine tetraacetate, 5-13mg/L citric acid and 0.05-0.15mg/L FeSO4•7H2O、0.15-0.35g/L NaHCO3、0.03-0.1g/LCaCl2、0.05-0.2g/L MgSO4•7H2O、1-3.5g/L NaNO3、0.05-0.15g/L K2HPO4、0.2-1g/L KH2PO4、1.5-2.5mg/L MnCl2•4H2O、0.02-0.05mg/L ZnSO4•7H2O、0.05-0.1mg/L CuSO4•5H2O、0.02-0.05mg/L Na2MoO4•2H2O、2-4mg/L H3BO3；
The expanding cultivation process in the first step comprises the following steps: selecting and separating a single microalgae cell, and performing stage expansion cultivation on the single microalgae cell, wherein in each stage of expansion cultivation process, when the number of the microalgae cells is more than or equal to 106/mL, the next stage of expansion cultivation process is performed, namely the single microalgae cell is cultivated in 15mL of culture medium solution for 10-15 days, 15mL of algae solution → transfer inoculation is performed to 85mL of culture medium solution for continuous cultivation for 10-15 days, 100mL of algae solution → transfer inoculation is performed to 0.9L of culture medium solution for continuous cultivation for 10-15 days, 1L of algae solution → transfer inoculation is performed to 4L of culture medium solution for continuous cultivation for 10-15 days, 5L of algae solution → transfer inoculation is performed to 13L of culture medium solution for continuous cultivation for 10-15 days, 18L of algae solution → transfer inoculation is performed to 782L of culture medium solution for continuous cultivation for 10-15 days, and 800L of algae solution is obtained;
the extraction process of the algae extract in the third step comprises the following steps: centrifuging and collecting algae cells of Bifidobacterium minutissima, Chlorella vulgaris and anabaena respectively, continuously washing with distilled water for 3 times, freeze-drying at-50 deg.C, storing for use, weighing appropriate amount of freeze-dried powder of algae of three kinds, and adding appropriate amount of PBS buffer solution with pH of 7.0 to obtain algae extract with density of 1.0 g/L;
the extraction process of the selenium, zinc and iron element extracts in the three steps comprises the steps of respectively mashing oyster mushroom, oyster meat and sedum aizoon, centrifuging and filtering to obtain filtrate, namely the corresponding extracts.
The performance parameters of the active cell microalgae repairing solutions prepared in examples 1 to 3 were respectively detected and compared with the existing commonly used microalgae repairing solutions, and the test results are shown in the following table:
| nitrogen fixation Rate (%) | Insecticidal and bactericidal rate (%) | Soil restoration Rate (%) | |
| Example 1 | 59.03 | 90.12 | 64.34 |
| Example 2 | 65.14 | 95.40 | 70.06 |
| Example 3 | 62.35 | 93.26 | 65.32 |
| Comparative example | 43.28 | 76.84 | 49.75 |
As can be seen from the comparison of the data in the table: when the active cell microalgae repairing liquid prepared in the embodiment 1-3 is actually used, the fertilizer efficiency of soil can be improved, the active cell microalgae repairing liquid has the effects of killing insects and bacteria, killing bacteria in the soil and on the surface of the soil, improving the decomposition capability of the soil and decomposing organic matters, so that the soil repairing efficiency is high, the growth environment of crops is improved, and the yield is increased.
Finally, it should be noted that: the above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that are within the spirit and principle of the present invention are intended to be included in the scope of the present invention.
Claims (10)
1. An active cell microalgae repairing liquid is characterized in that: the biological microalgae culture medium comprises a plurality of microalgae in a living state, wherein the added microalgae comprise 10-50 parts of Haematococcus, 5-40 parts of blue algae, 10-30 parts of stonewort and 30-50 parts of Goldfish algae according to the individual number of active cells of the microalgae, and 5 parts of a plant growth regulator is also added according to the parts by weight.
2. The active cell microalgae repairing fluid as claimed in claim 1, which is characterized in that: according to the individual quantity of the microalgae active cells, the added microalgae comprise 5-35 parts of the red hair algae, 10-30 parts of the blue algae, 10-25 parts of the stonewort and 35-50 parts of the goldfish algae.
3. The active cell microalgae repairing fluid as claimed in claim 1, which is characterized in that: the plant growth regulator in the raw materials comprises three types of plant growth regulators for improving amino acid content, protein content and stress resistance, and the plant growth regulator for improving the amino acid content comprises the following components: plant growth regulator for increasing protein content: stress-resistance-improving plant growth regulators =1: 2.
4. the active cell microalgae repairing fluid as claimed in claim 3, which is characterized in that: the plant growth regulator for increasing the amino acid content is prepared by mixing paclobutrazol, atrazine and indole mature ester, the plant growth regulator for increasing the protein content is prepared by mixing atrazine, simazine, atrazine and naphthylacetic acid, and the plant growth regulator for increasing the stress resistance is prepared by mixing abscisic acid, paclobutrazol, daminozide and chlormequat chloride.
5. The active cell microalgae repairing fluid as claimed in claim 1, which is characterized in that: the preparation raw materials of the repairing liquid also comprise algae extracts and selenium, zinc and iron element extracts, wherein the algae raw materials in the algae extracts are the bifidobacterium brevicornum, the chlorella vulgaris and the anabaena, and the parent raw materials of the selenium, zinc and iron element extracts are oyster mushroom, oyster meat and sedum.
6. A method for preparing the active cell microalgae repairing liquid according to any one of claims 1 to 5, which is characterized by comprising the following steps:
the method comprises the following steps: respectively carrying out expanded cultivation on the Rhodophyta, the blue algae, the stonewort and the Goldfish algae to obtain four microalgae solutions for later use;
step two: preparing three types of plant growth regulators capable of improving the amino acid content, the protein content and the stress resistance for later use;
step three: preparing algae extract and selenium, zinc and iron element extract for later use;
step four: and (3) fully mixing the four microalgae solutions in the steps with a plant growth regulator, the algae extract and the selenium, zinc and iron element extracts in sequence to obtain the active cell microalgae repairing solution.
7. The method for preparing the active cell microalgae repairing liquid as claimed in claim 6, which is characterized in that: the culture medium solution for expanding culture in the first step comprises the following components in concentration: 2-6mg/L disodium ethylene diamine tetraacetate, 5-13mg/L citric acid and 0.05-0.15mg/L FeSO4•7H2O、0.15-0.35g/L NaHCO3、0.03-0.1g/L CaCl2、0.05-0.2g/L MgSO4•7H2O、1-3.5g/L NaNO3、0.05-0.15g/L K2HPO4、0.2-1g/L KH2PO4、1.5-2.5mg/L MnCl2•4H2O、0.02-0.05mg/L ZnSO4•7H2O、0.05-0.1mg/L CuSO4•5H2O、0.02-0.05mg/L Na2MoO4•2H2O、2-4mg/L H3BO3。
8. The method for preparing the active cell microalgae repairing liquid as claimed in claim 6, which is characterized in that: the expanding cultivation process in the first step comprises the following steps: selecting and separating single microalgae cells, and carrying out hierarchical expansion cultivation on the single microalgae cells, wherein in each stage of expansion cultivation process, when the number of the microalgae cells is more than or equal to 106/mL, the next stage of expansion cultivation process is carried out, namely the single microalgae cells are cultivated in 15mL of culture medium solution for 10-15 days, 15mL of algae solution → transfer inoculation is carried out in 85mL of culture medium solution for continuous cultivation for 10-15 days, 100mL of algae solution is obtained → transfer inoculation is carried out in 0.9L of culture medium solution for continuous cultivation for 10-15 days, 1L of algae solution → transfer inoculation is carried out in 4L of culture medium solution for continuous cultivation for 10-15 days, 5L of algae solution is obtained → transfer inoculation is carried out in 13L of culture medium solution for continuous cultivation for 10-15 days, 18L of algae solution → transfer inoculation is carried out in 782L of culture medium solution for continuous cultivation for 10-15 days, and 800L of algae solution is obtained.
9. The method for preparing the active cell microalgae repairing liquid as claimed in claim 6, which is characterized in that: the extraction process of the algae extract in the third step comprises the following steps: centrifuging and collecting algae cells of Bifidobacterium minutissima, Chlorella vulgaris and Anabaena for 3 times, continuously washing with distilled water, lyophilizing at-50 deg.C, storing for use, weighing appropriate amount of lyophilized powder of algae of three kinds, and adding appropriate amount of PBS buffer solution with pH of 7.0 to obtain algae extract with density of 1.0 g/L.
10. The method for preparing the active cell microalgae repairing liquid as claimed in claim 6, which is characterized in that: the extraction process of the selenium, zinc and iron element extracts in the three steps comprises the steps of respectively mashing oyster mushroom, oyster meat and sedum aizoon, centrifuging and filtering to obtain filtrate, namely the corresponding extracts.
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| CN114804968A (en) * | 2022-06-06 | 2022-07-29 | 日照职业技术学院 | Organic ecological fertilizer containing microalgae and preparation method thereof |
| CN117303946A (en) * | 2023-09-11 | 2023-12-29 | 哈尔滨师范大学 | Composite microalgae fertilizer capable of improving salt stress resistance of crops |
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| CN117303946A (en) * | 2023-09-11 | 2023-12-29 | 哈尔滨师范大学 | Composite microalgae fertilizer capable of improving salt stress resistance of crops |
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