CN111423499A - 调控植物抗氧化能力和耐盐性的盐芥转录因子EsbZip60及其编码基因和应用 - Google Patents
调控植物抗氧化能力和耐盐性的盐芥转录因子EsbZip60及其编码基因和应用 Download PDFInfo
- Publication number
- CN111423499A CN111423499A CN202010193833.XA CN202010193833A CN111423499A CN 111423499 A CN111423499 A CN 111423499A CN 202010193833 A CN202010193833 A CN 202010193833A CN 111423499 A CN111423499 A CN 111423499A
- Authority
- CN
- China
- Prior art keywords
- esbzip60
- transcription factor
- salt
- plants
- coding gene
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 241000196324 Embryophyta Species 0.000 title claims abstract description 61
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 59
- 108091023040 Transcription factor Proteins 0.000 title claims abstract description 27
- 102000040945 Transcription factor Human genes 0.000 title claims abstract description 27
- 241000391074 Eutrema halophilum Species 0.000 title claims abstract description 22
- 230000003078 antioxidant effect Effects 0.000 title claims abstract description 19
- 239000003963 antioxidant agent Substances 0.000 title claims abstract description 18
- 230000015784 hyperosmotic salinity response Effects 0.000 title claims abstract description 18
- 230000001105 regulatory effect Effects 0.000 title claims abstract description 13
- 230000001276 controlling effect Effects 0.000 title claims abstract description 7
- 150000003839 salts Chemical class 0.000 claims abstract description 20
- 238000000034 method Methods 0.000 claims abstract description 4
- 125000003275 alpha amino acid group Chemical group 0.000 claims abstract 2
- 239000013604 expression vector Substances 0.000 claims description 11
- 241000219198 Brassica Species 0.000 claims description 10
- 235000003351 Brassica cretica Nutrition 0.000 claims description 10
- 235000003343 Brassica rupestris Nutrition 0.000 claims description 10
- QKSKPIVNLNLAAV-UHFFFAOYSA-N bis(2-chloroethyl) sulfide Chemical compound ClCCSCCCl QKSKPIVNLNLAAV-UHFFFAOYSA-N 0.000 claims description 10
- 235000010460 mustard Nutrition 0.000 claims description 10
- 238000003259 recombinant expression Methods 0.000 claims description 6
- 241000219194 Arabidopsis Species 0.000 claims description 5
- 239000002773 nucleotide Substances 0.000 claims description 4
- 125000003729 nucleotide group Chemical group 0.000 claims description 4
- 244000061176 Nicotiana tabacum Species 0.000 abstract description 62
- 235000002637 Nicotiana tabacum Nutrition 0.000 abstract description 62
- 230000009261 transgenic effect Effects 0.000 abstract description 54
- 102000004169 proteins and genes Human genes 0.000 abstract description 7
- 239000000463 material Substances 0.000 abstract description 4
- 240000008067 Cucumis sativus Species 0.000 abstract description 3
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 abstract description 3
- 235000007688 Lycopersicon esculentum Nutrition 0.000 abstract description 3
- 240000003768 Solanum lycopersicum Species 0.000 abstract description 3
- 244000061456 Solanum tuberosum Species 0.000 abstract description 3
- 235000002595 Solanum tuberosum Nutrition 0.000 abstract description 3
- 241000209140 Triticum Species 0.000 abstract description 3
- 235000021307 Triticum Nutrition 0.000 abstract description 3
- 238000009395 breeding Methods 0.000 abstract description 3
- 230000001488 breeding effect Effects 0.000 abstract description 3
- 230000002708 enhancing effect Effects 0.000 abstract description 3
- 230000003647 oxidation Effects 0.000 abstract description 2
- 238000007254 oxidation reaction Methods 0.000 abstract description 2
- 230000008569 process Effects 0.000 abstract description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 41
- 230000000694 effects Effects 0.000 description 27
- 210000001519 tissue Anatomy 0.000 description 25
- 239000011780 sodium chloride Substances 0.000 description 20
- 102000003992 Peroxidases Human genes 0.000 description 18
- 230000012010 growth Effects 0.000 description 14
- 230000035882 stress Effects 0.000 description 14
- 108040007629 peroxidase activity proteins Proteins 0.000 description 13
- 102000019197 Superoxide Dismutase Human genes 0.000 description 8
- 108010012715 Superoxide dismutase Proteins 0.000 description 8
- 239000013598 vector Substances 0.000 description 8
- 102000016938 Catalase Human genes 0.000 description 7
- 108010053835 Catalase Proteins 0.000 description 7
- 102000004190 Enzymes Human genes 0.000 description 7
- 108090000790 Enzymes Proteins 0.000 description 7
- 108010063907 Glutathione Reductase Proteins 0.000 description 7
- 102000006587 Glutathione peroxidase Human genes 0.000 description 7
- 108700016172 Glutathione peroxidases Proteins 0.000 description 7
- 102100036442 Glutathione reductase, mitochondrial Human genes 0.000 description 7
- 210000004027 cell Anatomy 0.000 description 7
- 238000003752 polymerase chain reaction Methods 0.000 description 7
- 108020004414 DNA Proteins 0.000 description 6
- 241001002356 Valeriana edulis Species 0.000 description 6
- 239000002299 complementary DNA Substances 0.000 description 5
- 239000012634 fragment Substances 0.000 description 5
- 239000003550 marker Substances 0.000 description 5
- 238000012216 screening Methods 0.000 description 5
- 230000003827 upregulation Effects 0.000 description 5
- 241001250566 Eutrema salsugineum Species 0.000 description 4
- 240000007594 Oryza sativa Species 0.000 description 4
- 239000013612 plasmid Substances 0.000 description 4
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 3
- 241000589158 Agrobacterium Species 0.000 description 3
- 108010001572 Basic-Leucine Zipper Transcription Factors Proteins 0.000 description 3
- 102000000806 Basic-Leucine Zipper Transcription Factors Human genes 0.000 description 3
- -1 COR15a Proteins 0.000 description 3
- 108091026890 Coding region Proteins 0.000 description 3
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 3
- 108091026898 Leader sequence (mRNA) Proteins 0.000 description 3
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 3
- 235000007164 Oryza sativa Nutrition 0.000 description 3
- 108091036066 Three prime untranslated region Proteins 0.000 description 3
- 150000001413 amino acids Chemical group 0.000 description 3
- 239000003623 enhancer Substances 0.000 description 3
- 238000001976 enzyme digestion Methods 0.000 description 3
- 230000014509 gene expression Effects 0.000 description 3
- 239000001963 growth medium Substances 0.000 description 3
- 239000001301 oxygen Substances 0.000 description 3
- 229910052760 oxygen Inorganic materials 0.000 description 3
- 238000010839 reverse transcription Methods 0.000 description 3
- 235000009566 rice Nutrition 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 108700024394 Exon Proteins 0.000 description 2
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 2
- 108700026244 Open Reading Frames Proteins 0.000 description 2
- 238000012408 PCR amplification Methods 0.000 description 2
- 108091081024 Start codon Proteins 0.000 description 2
- 108090000848 Ubiquitin Proteins 0.000 description 2
- 102000044159 Ubiquitin Human genes 0.000 description 2
- 240000008042 Zea mays Species 0.000 description 2
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 2
- 125000000539 amino acid group Chemical group 0.000 description 2
- 230000003321 amplification Effects 0.000 description 2
- 108010040443 aspartyl-aspartic acid Proteins 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 230000033228 biological regulation Effects 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 description 2
- 229960003180 glutathione Drugs 0.000 description 2
- 108020004999 messenger RNA Proteins 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- 230000008488 polyadenylation Effects 0.000 description 2
- 230000004044 response Effects 0.000 description 2
- 108091008146 restriction endonucleases Proteins 0.000 description 2
- 108010026333 seryl-proline Proteins 0.000 description 2
- 230000001988 toxicity Effects 0.000 description 2
- 231100000419 toxicity Toxicity 0.000 description 2
- 230000005026 transcription initiation Effects 0.000 description 2
- 108020005345 3' Untranslated Regions Proteins 0.000 description 1
- 241000589155 Agrobacterium tumefaciens Species 0.000 description 1
- RLMISHABBKUNFO-WHFBIAKZSA-N Ala-Ala-Gly Chemical compound C[C@H](N)C(=O)N[C@@H](C)C(=O)NCC(O)=O RLMISHABBKUNFO-WHFBIAKZSA-N 0.000 description 1
- FXKNPWNXPQZLES-ZLUOBGJFSA-N Ala-Asn-Ser Chemical compound [H]N[C@@H](C)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CO)C(O)=O FXKNPWNXPQZLES-ZLUOBGJFSA-N 0.000 description 1
- GWFSQQNGMPGBEF-GHCJXIJMSA-N Ala-Asp-Ile Chemical compound CC[C@H](C)[C@@H](C(=O)O)NC(=O)[C@H](CC(=O)O)NC(=O)[C@H](C)N GWFSQQNGMPGBEF-GHCJXIJMSA-N 0.000 description 1
- MKZCBYZBCINNJN-DLOVCJGASA-N Ala-Asp-Phe Chemical compound C[C@H](N)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@H](C(O)=O)CC1=CC=CC=C1 MKZCBYZBCINNJN-DLOVCJGASA-N 0.000 description 1
- VCSABYLVNWQYQE-UHFFFAOYSA-N Ala-Lys-Lys Natural products NCCCCC(NC(=O)C(N)C)C(=O)NC(CCCCN)C(O)=O VCSABYLVNWQYQE-UHFFFAOYSA-N 0.000 description 1
- MDNAVFBZPROEHO-DCAQKATOSA-N Ala-Lys-Val Chemical compound [H]N[C@@H](C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C(C)C)C(O)=O MDNAVFBZPROEHO-DCAQKATOSA-N 0.000 description 1
- MDNAVFBZPROEHO-UHFFFAOYSA-N Ala-Lys-Val Natural products CC(C)C(C(O)=O)NC(=O)C(NC(=O)C(C)N)CCCCN MDNAVFBZPROEHO-UHFFFAOYSA-N 0.000 description 1
- KLALXKYLOMZDQT-ZLUOBGJFSA-N Ala-Ser-Asn Chemical compound C[C@H](N)C(=O)N[C@@H](CO)C(=O)N[C@H](C(O)=O)CC(N)=O KLALXKYLOMZDQT-ZLUOBGJFSA-N 0.000 description 1
- 241000219195 Arabidopsis thaliana Species 0.000 description 1
- 101100321977 Arabidopsis thaliana CYP707A3 gene Proteins 0.000 description 1
- 101100082457 Arabidopsis thaliana PBL2 gene Proteins 0.000 description 1
- 101100194010 Arabidopsis thaliana RD29A gene Proteins 0.000 description 1
- UFBURHXMKFQVLM-CIUDSAMLSA-N Arg-Glu-Ser Chemical compound [H]N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CO)C(O)=O UFBURHXMKFQVLM-CIUDSAMLSA-N 0.000 description 1
- BTJVOUQWFXABOI-IHRRRGAJSA-N Arg-Lys-Lys Chemical compound NCCCC[C@@H](C(O)=O)NC(=O)[C@H](CCCCN)NC(=O)[C@@H](N)CCCNC(N)=N BTJVOUQWFXABOI-IHRRRGAJSA-N 0.000 description 1
- DNLQVHBBMPZUGJ-BQBZGAKWSA-N Arg-Ser-Gly Chemical compound [H]N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CO)C(=O)NCC(O)=O DNLQVHBBMPZUGJ-BQBZGAKWSA-N 0.000 description 1
- FRBAHXABMQXSJQ-FXQIFTODSA-N Arg-Ser-Ser Chemical compound [H]N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CO)C(=O)N[C@@H](CO)C(O)=O FRBAHXABMQXSJQ-FXQIFTODSA-N 0.000 description 1
- 101100269812 Artemisia annua AMS1 gene Proteins 0.000 description 1
- 101100323110 Artemisia annua CYP71AV1 gene Proteins 0.000 description 1
- ACRYGQFHAQHDSF-ZLUOBGJFSA-N Asn-Asn-Asn Chemical compound NC(=O)C[C@H](N)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC(N)=O)C(O)=O ACRYGQFHAQHDSF-ZLUOBGJFSA-N 0.000 description 1
- BHQQRVARKXWXPP-ACZMJKKPSA-N Asn-Asp-Glu Chemical compound C(CC(=O)O)[C@@H](C(=O)O)NC(=O)[C@H](CC(=O)O)NC(=O)[C@H](CC(=O)N)N BHQQRVARKXWXPP-ACZMJKKPSA-N 0.000 description 1
- NAPNAGZWHQHZLG-ZLUOBGJFSA-N Asp-Asp-Ala Chemical compound C[C@@H](C(=O)O)NC(=O)[C@H](CC(=O)O)NC(=O)[C@H](CC(=O)O)N NAPNAGZWHQHZLG-ZLUOBGJFSA-N 0.000 description 1
- SBHUBSDEZQFJHJ-CIUDSAMLSA-N Asp-Asp-Leu Chemical compound CC(C)C[C@@H](C(O)=O)NC(=O)[C@H](CC(O)=O)NC(=O)[C@@H](N)CC(O)=O SBHUBSDEZQFJHJ-CIUDSAMLSA-N 0.000 description 1
- PXLNPFOJZQMXAT-BYULHYEWSA-N Asp-Asp-Val Chemical compound CC(C)[C@@H](C(O)=O)NC(=O)[C@H](CC(O)=O)NC(=O)[C@@H](N)CC(O)=O PXLNPFOJZQMXAT-BYULHYEWSA-N 0.000 description 1
- LJRPYAZQQWHEEV-FXQIFTODSA-N Asp-Gln-Gln Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCC(N)=O)C(O)=O LJRPYAZQQWHEEV-FXQIFTODSA-N 0.000 description 1
- JUWZKMBALYLZCK-WHFBIAKZSA-N Asp-Gly-Asn Chemical compound OC(=O)C[C@H](N)C(=O)NCC(=O)N[C@@H](CC(N)=O)C(O)=O JUWZKMBALYLZCK-WHFBIAKZSA-N 0.000 description 1
- PCJOFZYFFMBZKC-PCBIJLKTSA-N Asp-Phe-Ile Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H]([C@@H](C)CC)C(O)=O PCJOFZYFFMBZKC-PCBIJLKTSA-N 0.000 description 1
- KGHLGJAXYSVNJP-WHFBIAKZSA-N Asp-Ser-Gly Chemical compound OC(=O)C[C@H](N)C(=O)N[C@@H](CO)C(=O)NCC(O)=O KGHLGJAXYSVNJP-WHFBIAKZSA-N 0.000 description 1
- JSHWXQIZOCVWIA-ZKWXMUAHSA-N Asp-Ser-Val Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H](CO)C(=O)N[C@@H](C(C)C)C(O)=O JSHWXQIZOCVWIA-ZKWXMUAHSA-N 0.000 description 1
- 101150009336 CYP707A1 gene Proteins 0.000 description 1
- 241000701489 Cauliflower mosaic virus Species 0.000 description 1
- 108091062157 Cis-regulatory element Proteins 0.000 description 1
- CIVXDCMSSFGWAL-YUMQZZPRSA-N Cys-Lys-Gly Chemical compound C(CCN)C[C@@H](C(=O)NCC(=O)O)NC(=O)[C@H](CS)N CIVXDCMSSFGWAL-YUMQZZPRSA-N 0.000 description 1
- XSQAWJCVYDEWPT-GUBZILKMSA-N Cys-Met-Arg Chemical compound SC[C@H](N)C(=O)N[C@@H](CCSC)C(=O)N[C@H](C(O)=O)CCCN=C(N)N XSQAWJCVYDEWPT-GUBZILKMSA-N 0.000 description 1
- LHJDLVVQRJIURS-SRVKXCTJSA-N Cys-Phe-Asp Chemical compound C1=CC=C(C=C1)C[C@@H](C(=O)N[C@@H](CC(=O)O)C(=O)O)NC(=O)[C@H](CS)N LHJDLVVQRJIURS-SRVKXCTJSA-N 0.000 description 1
- YYLBXQJGWOQZOU-IHRRRGAJSA-N Cys-Phe-Val Chemical compound CC(C)[C@@H](C(=O)O)NC(=O)[C@H](CC1=CC=CC=C1)NC(=O)[C@H](CS)N YYLBXQJGWOQZOU-IHRRRGAJSA-N 0.000 description 1
- 101150078760 ERF5 gene Proteins 0.000 description 1
- 241000620209 Escherichia coli DH5[alpha] Species 0.000 description 1
- 229930182566 Gentamicin Natural products 0.000 description 1
- CEAZRRDELHUEMR-URQXQFDESA-N Gentamicin Chemical compound O1[C@H](C(C)NC)CC[C@@H](N)[C@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](NC)[C@@](C)(O)CO2)O)[C@H](N)C[C@@H]1N CEAZRRDELHUEMR-URQXQFDESA-N 0.000 description 1
- IKDOHQHEFPPGJG-FXQIFTODSA-N Gln-Asp-Glu Chemical compound [H]N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(O)=O IKDOHQHEFPPGJG-FXQIFTODSA-N 0.000 description 1
- LWDGZZGWDMHBOF-FXQIFTODSA-N Gln-Glu-Asn Chemical compound [H]N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(N)=O)C(O)=O LWDGZZGWDMHBOF-FXQIFTODSA-N 0.000 description 1
- KHNJVFYHIKLUPD-SRVKXCTJSA-N Gln-Leu-Met Chemical compound CC(C)C[C@@H](C(=O)N[C@@H](CCSC)C(=O)O)NC(=O)[C@H](CCC(=O)N)N KHNJVFYHIKLUPD-SRVKXCTJSA-N 0.000 description 1
- SXGMGNZEHFORAV-IUCAKERBSA-N Gln-Lys-Gly Chemical compound C(CCN)C[C@@H](C(=O)NCC(=O)O)NC(=O)[C@H](CCC(=O)N)N SXGMGNZEHFORAV-IUCAKERBSA-N 0.000 description 1
- CGYDXNKRIMJMLV-GUBZILKMSA-N Glu-Arg-Glu Chemical compound [H]N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCC(O)=O)C(O)=O CGYDXNKRIMJMLV-GUBZILKMSA-N 0.000 description 1
- SVZIKUHLRKVZIF-GUBZILKMSA-N Glu-Asn-His Chemical compound C1=C(NC=N1)C[C@@H](C(=O)O)NC(=O)[C@H](CC(=O)N)NC(=O)[C@H](CCC(=O)O)N SVZIKUHLRKVZIF-GUBZILKMSA-N 0.000 description 1
- HRBYTAIBKPNZKQ-AVGNSLFASA-N Glu-Lys-Lys Chemical compound NCCCC[C@@H](C(O)=O)NC(=O)[C@H](CCCCN)NC(=O)[C@@H](N)CCC(O)=O HRBYTAIBKPNZKQ-AVGNSLFASA-N 0.000 description 1
- YRMZCZIRHYCNHX-RYUDHWBXSA-N Glu-Phe-Gly Chemical compound [H]N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)NCC(O)=O YRMZCZIRHYCNHX-RYUDHWBXSA-N 0.000 description 1
- QNJNPKSWAHPYGI-JYJNAYRXSA-N Glu-Phe-Leu Chemical compound OC(=O)CC[C@H](N)C(=O)N[C@H](C(=O)N[C@@H](CC(C)C)C(O)=O)CC1=CC=CC=C1 QNJNPKSWAHPYGI-JYJNAYRXSA-N 0.000 description 1
- DXVOKNVIKORTHQ-GUBZILKMSA-N Glu-Pro-Glu Chemical compound [H]N[C@@H](CCC(O)=O)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCC(O)=O)C(O)=O DXVOKNVIKORTHQ-GUBZILKMSA-N 0.000 description 1
- BPLNJYHNAJVLRT-ACZMJKKPSA-N Glu-Ser-Ala Chemical compound [H]N[C@@H](CCC(O)=O)C(=O)N[C@@H](CO)C(=O)N[C@@H](C)C(O)=O BPLNJYHNAJVLRT-ACZMJKKPSA-N 0.000 description 1
- HMJULNMJWOZNFI-XHNCKOQMSA-N Glu-Ser-Pro Chemical compound C1C[C@@H](N(C1)C(=O)[C@H](CO)NC(=O)[C@H](CCC(=O)O)N)C(=O)O HMJULNMJWOZNFI-XHNCKOQMSA-N 0.000 description 1
- VNCNWQPIQYAMAK-ACZMJKKPSA-N Glu-Ser-Ser Chemical compound [H]N[C@@H](CCC(O)=O)C(=O)N[C@@H](CO)C(=O)N[C@@H](CO)C(O)=O VNCNWQPIQYAMAK-ACZMJKKPSA-N 0.000 description 1
- HBMRTXJZQDVRFT-DZKIICNBSA-N Glu-Tyr-Val Chemical compound [H]N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC1=CC=C(O)C=C1)C(=O)N[C@@H](C(C)C)C(O)=O HBMRTXJZQDVRFT-DZKIICNBSA-N 0.000 description 1
- 108010053070 Glutathione Disulfide Proteins 0.000 description 1
- XBWMTPAIUQIWKA-BYULHYEWSA-N Gly-Asp-Ile Chemical compound CC[C@H](C)[C@@H](C(O)=O)NC(=O)[C@H](CC(O)=O)NC(=O)CN XBWMTPAIUQIWKA-BYULHYEWSA-N 0.000 description 1
- OHUKZZYSJBKFRR-WHFBIAKZSA-N Gly-Ser-Asp Chemical compound [H]NCC(=O)N[C@@H](CO)C(=O)N[C@@H](CC(O)=O)C(O)=O OHUKZZYSJBKFRR-WHFBIAKZSA-N 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- FHCNLXMTQJNJNH-KBIXCLLPSA-N Ile-Cys-Gln Chemical compound N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCC(N)=O)C(=O)O FHCNLXMTQJNJNH-KBIXCLLPSA-N 0.000 description 1
- SPQWWEZBHXHUJN-KBIXCLLPSA-N Ile-Glu-Ser Chemical compound CC[C@H](C)[C@H](N)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CO)C(O)=O SPQWWEZBHXHUJN-KBIXCLLPSA-N 0.000 description 1
- 108020005350 Initiator Codon Proteins 0.000 description 1
- 102100034343 Integrase Human genes 0.000 description 1
- 101150093335 KIN1 gene Proteins 0.000 description 1
- 241000880493 Leptailurus serval Species 0.000 description 1
- PPBKJAQJAUHZKX-SRVKXCTJSA-N Leu-Cys-Leu Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H](CS)C(=O)N[C@H](C(O)=O)CC(C)C PPBKJAQJAUHZKX-SRVKXCTJSA-N 0.000 description 1
- BABSVXFGKFLIGW-UWVGGRQHSA-N Leu-Gly-Arg Chemical compound CC(C)C[C@H](N)C(=O)NCC(=O)N[C@H](C(O)=O)CCCNC(N)=N BABSVXFGKFLIGW-UWVGGRQHSA-N 0.000 description 1
- PDQDCFBVYXEFSD-SRVKXCTJSA-N Leu-Leu-Asp Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(O)=O)C(O)=O PDQDCFBVYXEFSD-SRVKXCTJSA-N 0.000 description 1
- QNBVTHNJGCOVFA-AVGNSLFASA-N Leu-Leu-Glu Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@H](C(O)=O)CCC(O)=O QNBVTHNJGCOVFA-AVGNSLFASA-N 0.000 description 1
- YOKVEHGYYQEQOP-QWRGUYRKSA-N Leu-Leu-Gly Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H](CC(C)C)C(=O)NCC(O)=O YOKVEHGYYQEQOP-QWRGUYRKSA-N 0.000 description 1
- FBNPMTNBFFAMMH-UHFFFAOYSA-N Leu-Val-Arg Natural products CC(C)CC(N)C(=O)NC(C(C)C)C(=O)NC(C(O)=O)CCCN=C(N)N FBNPMTNBFFAMMH-UHFFFAOYSA-N 0.000 description 1
- 108060001084 Luciferase Proteins 0.000 description 1
- IMAKMJCBYCSMHM-AVGNSLFASA-N Lys-Glu-Lys Chemical compound NCCCC[C@H](N)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@H](C(O)=O)CCCCN IMAKMJCBYCSMHM-AVGNSLFASA-N 0.000 description 1
- VEGLGAOVLFODGC-GUBZILKMSA-N Lys-Glu-Ser Chemical compound [H]N[C@@H](CCCCN)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CO)C(O)=O VEGLGAOVLFODGC-GUBZILKMSA-N 0.000 description 1
- LJADEBULDNKJNK-IHRRRGAJSA-N Lys-Leu-Val Chemical compound CC(C)C[C@H](NC(=O)[C@@H](N)CCCCN)C(=O)N[C@@H](C(C)C)C(O)=O LJADEBULDNKJNK-IHRRRGAJSA-N 0.000 description 1
- MGKFCQFVPKOWOL-CIUDSAMLSA-N Lys-Ser-Asp Chemical compound C(CCN)C[C@@H](C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(=O)O)C(=O)O)N MGKFCQFVPKOWOL-CIUDSAMLSA-N 0.000 description 1
- CTJUSALVKAWFFU-CIUDSAMLSA-N Lys-Ser-Cys Chemical compound C(CCN)C[C@@H](C(=O)N[C@@H](CO)C(=O)N[C@@H](CS)C(=O)O)N CTJUSALVKAWFFU-CIUDSAMLSA-N 0.000 description 1
- UGCIQUYEJIEHKX-GVXVVHGQSA-N Lys-Val-Glu Chemical compound [H]N[C@@H](CCCCN)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCC(O)=O)C(O)=O UGCIQUYEJIEHKX-GVXVVHGQSA-N 0.000 description 1
- 240000004658 Medicago sativa Species 0.000 description 1
- 235000017587 Medicago sativa ssp. sativa Nutrition 0.000 description 1
- ONGCSGVHCSAATF-CIUDSAMLSA-N Met-Ala-Glu Chemical compound CSCC[C@H](N)C(=O)N[C@@H](C)C(=O)N[C@H](C(O)=O)CCC(O)=O ONGCSGVHCSAATF-CIUDSAMLSA-N 0.000 description 1
- QAHFGYLFLVGBNW-DCAQKATOSA-N Met-Ala-Lys Chemical compound CSCC[C@H](N)C(=O)N[C@@H](C)C(=O)N[C@H](C(O)=O)CCCCN QAHFGYLFLVGBNW-DCAQKATOSA-N 0.000 description 1
- HGAJNEWOUHDUMZ-SRVKXCTJSA-N Met-Leu-Glu Chemical compound CSCC[C@H](N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@H](C(O)=O)CCC(O)=O HGAJNEWOUHDUMZ-SRVKXCTJSA-N 0.000 description 1
- YBAFDPFAUTYYRW-UHFFFAOYSA-N N-L-alpha-glutamyl-L-leucine Natural products CC(C)CC(C(O)=O)NC(=O)C(N)CCC(O)=O YBAFDPFAUTYYRW-UHFFFAOYSA-N 0.000 description 1
- XZFYRXDAULDNFX-UHFFFAOYSA-N N-L-cysteinyl-L-phenylalanine Natural products SCC(N)C(=O)NC(C(O)=O)CC1=CC=CC=C1 XZFYRXDAULDNFX-UHFFFAOYSA-N 0.000 description 1
- XMBSYZWANAQXEV-UHFFFAOYSA-N N-alpha-L-glutamyl-L-phenylalanine Natural products OC(=O)CCC(N)C(=O)NC(C(O)=O)CC1=CC=CC=C1 XMBSYZWANAQXEV-UHFFFAOYSA-N 0.000 description 1
- 108010002311 N-glycylglutamic acid Proteins 0.000 description 1
- BQVUABVGYYSDCJ-UHFFFAOYSA-N Nalpha-L-Leucyl-L-tryptophan Natural products C1=CC=C2C(CC(NC(=O)C(N)CC(C)C)C(O)=O)=CNC2=C1 BQVUABVGYYSDCJ-UHFFFAOYSA-N 0.000 description 1
- 101100119050 Nicotiana tabacum ERF3 gene Proteins 0.000 description 1
- 108700020962 Peroxidase Proteins 0.000 description 1
- MQVFHOPCKNTHGT-MELADBBJSA-N Phe-Asp-Pro Chemical compound C1C[C@@H](N(C1)C(=O)[C@H](CC(=O)O)NC(=O)[C@H](CC2=CC=CC=C2)N)C(=O)O MQVFHOPCKNTHGT-MELADBBJSA-N 0.000 description 1
- OPEVYHFJXLCCRT-AVGNSLFASA-N Phe-Gln-Ser Chemical compound [H]N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CO)C(O)=O OPEVYHFJXLCCRT-AVGNSLFASA-N 0.000 description 1
- KDYPMIZMXDECSU-JYJNAYRXSA-N Phe-Leu-Glu Chemical compound OC(=O)CC[C@@H](C(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](N)CC1=CC=CC=C1 KDYPMIZMXDECSU-JYJNAYRXSA-N 0.000 description 1
- KAJLHCWRWDSROH-BZSNNMDCSA-N Phe-Phe-Asp Chemical compound C([C@H](N)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CC(O)=O)C(O)=O)C1=CC=CC=C1 KAJLHCWRWDSROH-BZSNNMDCSA-N 0.000 description 1
- IAOZOFPONWDXNT-IXOXFDKPSA-N Phe-Ser-Thr Chemical compound [H]N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H](CO)C(=O)N[C@@H]([C@@H](C)O)C(O)=O IAOZOFPONWDXNT-IXOXFDKPSA-N 0.000 description 1
- JSGWNFKWZNPDAV-YDHLFZDLSA-N Phe-Val-Asp Chemical compound OC(=O)C[C@@H](C(O)=O)NC(=O)[C@H](C(C)C)NC(=O)[C@@H](N)CC1=CC=CC=C1 JSGWNFKWZNPDAV-YDHLFZDLSA-N 0.000 description 1
- 108700001094 Plant Genes Proteins 0.000 description 1
- ZCXQTRXYZOSGJR-FXQIFTODSA-N Pro-Asp-Ser Chemical compound [H]N1CCC[C@H]1C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CO)C(O)=O ZCXQTRXYZOSGJR-FXQIFTODSA-N 0.000 description 1
- XZONQWUEBAFQPO-HJGDQZAQSA-N Pro-Gln-Thr Chemical compound [H]N1CCC[C@H]1C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(O)=O XZONQWUEBAFQPO-HJGDQZAQSA-N 0.000 description 1
- SSWJYJHXQOYTSP-SRVKXCTJSA-N Pro-His-Gln Chemical compound [H]N1CCC[C@H]1C(=O)N[C@@H](CC1=CNC=N1)C(=O)N[C@@H](CCC(N)=O)C(O)=O SSWJYJHXQOYTSP-SRVKXCTJSA-N 0.000 description 1
- IBGCFJDLCYTKPW-NAKRPEOUSA-N Pro-Ile-Ala Chemical compound OC(=O)[C@H](C)NC(=O)[C@H]([C@@H](C)CC)NC(=O)[C@@H]1CCCN1 IBGCFJDLCYTKPW-NAKRPEOUSA-N 0.000 description 1
- RMJZWERKFFNNNS-XGEHTFHBSA-N Pro-Thr-Ser Chemical compound [H]N1CCC[C@H]1C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CO)C(O)=O RMJZWERKFFNNNS-XGEHTFHBSA-N 0.000 description 1
- CXGLFEOYCJFKPR-RCWTZXSCSA-N Pro-Thr-Val Chemical compound [H]N1CCC[C@H]1C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](C(C)C)C(O)=O CXGLFEOYCJFKPR-RCWTZXSCSA-N 0.000 description 1
- IMNVAOPEMFDAQD-NHCYSSNCSA-N Pro-Val-Glu Chemical compound [H]N1CCC[C@H]1C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCC(O)=O)C(O)=O IMNVAOPEMFDAQD-NHCYSSNCSA-N 0.000 description 1
- 108091034057 RNA (poly(A)) Proteins 0.000 description 1
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 description 1
- NLQUOHDCLSFABG-GUBZILKMSA-N Ser-Arg-Arg Chemical compound NC(N)=NCCC[C@H](NC(=O)[C@H](CO)N)C(=O)N[C@@H](CCCN=C(N)N)C(O)=O NLQUOHDCLSFABG-GUBZILKMSA-N 0.000 description 1
- NRCJWSGXMAPYQX-LPEHRKFASA-N Ser-Arg-Pro Chemical compound C1C[C@@H](N(C1)C(=O)[C@H](CCCN=C(N)N)NC(=O)[C@H](CO)N)C(=O)O NRCJWSGXMAPYQX-LPEHRKFASA-N 0.000 description 1
- SQBLRDDJTUJDMV-ACZMJKKPSA-N Ser-Glu-Asn Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(N)=O)C(O)=O SQBLRDDJTUJDMV-ACZMJKKPSA-N 0.000 description 1
- BRGQQXQKPUCUJQ-KBIXCLLPSA-N Ser-Glu-Ile Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H]([C@@H](C)CC)C(O)=O BRGQQXQKPUCUJQ-KBIXCLLPSA-N 0.000 description 1
- BPMRXBZYPGYPJN-WHFBIAKZSA-N Ser-Gly-Asn Chemical compound [H]N[C@@H](CO)C(=O)NCC(=O)N[C@@H](CC(N)=O)C(O)=O BPMRXBZYPGYPJN-WHFBIAKZSA-N 0.000 description 1
- DJACUBDEDBZKLQ-KBIXCLLPSA-N Ser-Ile-Glu Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CCC(O)=O)C(O)=O DJACUBDEDBZKLQ-KBIXCLLPSA-N 0.000 description 1
- DOSZISJPMCYEHT-NAKRPEOUSA-N Ser-Ile-Val Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](C(C)C)C(O)=O DOSZISJPMCYEHT-NAKRPEOUSA-N 0.000 description 1
- QYSFWUIXDFJUDW-DCAQKATOSA-N Ser-Leu-Arg Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O QYSFWUIXDFJUDW-DCAQKATOSA-N 0.000 description 1
- VMLONWHIORGALA-SRVKXCTJSA-N Ser-Leu-Leu Chemical compound CC(C)C[C@@H](C([O-])=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H]([NH3+])CO VMLONWHIORGALA-SRVKXCTJSA-N 0.000 description 1
- LRWBCWGEUCKDTN-BJDJZHNGSA-N Ser-Lys-Ile Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)CC)C(O)=O LRWBCWGEUCKDTN-BJDJZHNGSA-N 0.000 description 1
- QJKPECIAWNNKIT-KKUMJFAQSA-N Ser-Lys-Tyr Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC1=CC=C(O)C=C1)C(O)=O QJKPECIAWNNKIT-KKUMJFAQSA-N 0.000 description 1
- QSHKTZVJGDVFEW-GUBZILKMSA-N Ser-Met-Met Chemical compound CSCC[C@@H](C(=O)N[C@@H](CCSC)C(=O)O)NC(=O)[C@H](CO)N QSHKTZVJGDVFEW-GUBZILKMSA-N 0.000 description 1
- JGUWRQWULDWNCM-FXQIFTODSA-N Ser-Val-Ser Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CO)C(O)=O JGUWRQWULDWNCM-FXQIFTODSA-N 0.000 description 1
- NLSNVZAREYQMGR-HJGDQZAQSA-N Thr-Asp-Leu Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(O)=O NLSNVZAREYQMGR-HJGDQZAQSA-N 0.000 description 1
- KBLYJPQSNGTDIU-LOKLDPHHSA-N Thr-Glu-Pro Chemical compound C[C@H]([C@@H](C(=O)N[C@@H](CCC(=O)O)C(=O)N1CCC[C@@H]1C(=O)O)N)O KBLYJPQSNGTDIU-LOKLDPHHSA-N 0.000 description 1
- MPUMPERGHHJGRP-WEDXCCLWSA-N Thr-Gly-Lys Chemical compound C[C@H]([C@@H](C(=O)NCC(=O)N[C@@H](CCCCN)C(=O)O)N)O MPUMPERGHHJGRP-WEDXCCLWSA-N 0.000 description 1
- CJXURNZYNHCYFD-WDCWCFNPSA-N Thr-Lys-Gln Chemical compound C[C@H]([C@@H](C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCC(=O)N)C(=O)O)N)O CJXURNZYNHCYFD-WDCWCFNPSA-N 0.000 description 1
- KIMOCKLJBXHFIN-YLVFBTJISA-N Trp-Ile-Gly Chemical compound C1=CC=C2C(C[C@H](N)C(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(O)=O)=CNC2=C1 KIMOCKLJBXHFIN-YLVFBTJISA-N 0.000 description 1
- DVLHKUWLNKDINO-PMVMPFDFSA-N Trp-Tyr-Leu Chemical compound [H]N[C@@H](CC1=CNC2=C1C=CC=C2)C(=O)N[C@@H](CC1=CC=C(O)C=C1)C(=O)N[C@@H](CC(C)C)C(O)=O DVLHKUWLNKDINO-PMVMPFDFSA-N 0.000 description 1
- 101100494966 Trypanosoma brucei brucei CRK1 gene Proteins 0.000 description 1
- 108091023045 Untranslated Region Proteins 0.000 description 1
- XGJLNBNZNMVJRS-NRPADANISA-N Val-Glu-Ala Chemical compound CC(C)[C@H](N)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](C)C(O)=O XGJLNBNZNMVJRS-NRPADANISA-N 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 1
- 230000036579 abiotic stress Effects 0.000 description 1
- 238000000246 agarose gel electrophoresis Methods 0.000 description 1
- KOSRFJWDECSPRO-UHFFFAOYSA-N alpha-L-glutamyl-L-glutamic acid Natural products OC(=O)CCC(N)C(=O)NC(CCC(O)=O)C(O)=O KOSRFJWDECSPRO-UHFFFAOYSA-N 0.000 description 1
- 229960000723 ampicillin Drugs 0.000 description 1
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 108010043240 arginyl-leucyl-glycine Proteins 0.000 description 1
- 108010069205 aspartyl-phenylalanine Proteins 0.000 description 1
- 108010068265 aspartyltyrosine Proteins 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 238000010170 biological method Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000004790 biotic stress Effects 0.000 description 1
- 210000002390 cell membrane structure Anatomy 0.000 description 1
- 230000007960 cellular response to stress Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 238000012364 cultivation method Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 239000005547 deoxyribonucleotide Substances 0.000 description 1
- 125000002637 deoxyribonucleotide group Chemical group 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000000539 dimer Substances 0.000 description 1
- 230000008641 drought stress Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 229960002518 gentamicin Drugs 0.000 description 1
- YPZRWBKMTBYPTK-BJDJZHNGSA-N glutathione disulfide Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@H](C(=O)NCC(O)=O)CSSC[C@@H](C(=O)NCC(O)=O)NC(=O)CC[C@H](N)C(O)=O YPZRWBKMTBYPTK-BJDJZHNGSA-N 0.000 description 1
- 108010050848 glycylleucine Proteins 0.000 description 1
- 101150054900 gus gene Proteins 0.000 description 1
- 230000002363 herbicidal effect Effects 0.000 description 1
- 239000004009 herbicide Substances 0.000 description 1
- 108010018006 histidylserine Proteins 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 229960000318 kanamycin Drugs 0.000 description 1
- 229930027917 kanamycin Natural products 0.000 description 1
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 1
- 229930182823 kanamycin A Natural products 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 108010009298 lysylglutamic acid Proteins 0.000 description 1
- 235000009973 maize Nutrition 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 238000000520 microinjection Methods 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 239000006870 ms-medium Substances 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 108010058731 nopaline synthase Proteins 0.000 description 1
- 210000003463 organelle Anatomy 0.000 description 1
- 230000002018 overexpression Effects 0.000 description 1
- 230000008557 oxygen metabolism Effects 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 108010051242 phenylalanylserine Proteins 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 239000013600 plasmid vector Substances 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 108010020432 prolyl-prolylisoleucine Proteins 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 239000003642 reactive oxygen metabolite Substances 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 230000019491 signal transduction Effects 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 230000002103 transcriptional effect Effects 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 230000001131 transforming effect Effects 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- 230000014621 translational initiation Effects 0.000 description 1
- 210000003934 vacuole Anatomy 0.000 description 1
- 108010073969 valyllysine Proteins 0.000 description 1
- 230000009614 wildtype growth Effects 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8273—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
本发明属于农业生物技术领域,具体涉及调控植物抗氧化能力和耐盐性的盐芥转录因子EsbZip60及其编码基因和应用,其氨基酸序列如SEQ ID No.1所示。本发明以对盐芥为实验材料,得到EsbZip60蛋白及其编码基因,并将该编码基因导入烟草,显著提升了植物的抗盐和抗氧化的能力。本发明的EsbZip60蛋白及其编码基因对改良、增强转基因植株包括小麦、土豆、黄瓜和番茄等经济作物的耐盐能力,并加速植物抗逆性改良的分子育种进程具有十分重要的理论和实践意义。
Description
技术领域
本发明属于农业生物技术领域,具体涉及调控植物抗氧化能力和耐盐性的盐芥转录因子EsbZip60及其编码基因和应用。
背景技术
bZIP转录因子是植物中最大的转录因子调控家族之一,其包含高度保守的DNA碱性结合结构域,以及多样性的亮氨酸拉链,bZIP蛋白以同源或异源二聚体的形式存在,其通过与顺式作用元件G-box、C-box、ABRE、LTRE和BoxII的结合,调控下游相关基因如ERF5、KIN1、COR15a、COR78、CYP707A1、CYP707A3、ADS和CYP71AV1的表达,进而在植物生物、非生物胁迫应答以及发育等生理过程中发挥重要作用。
植物中bZIPs家族的蛋白具有功能多样性。水稻OsHBP1b基因降低了转基因烟草中过氧化氢的含量,并增强了超氧化物歧化酶的活性,进而提高转基因植株细胞液泡膜的稳定性和K+/Na+比,表明OsHBP1b具有较强的抗氧化损伤功能。稻OsbZIP71被干旱胁迫强烈诱导,其过量表达时可显著提高转基因水稻的抗旱性。Oshox22过量表达则提高水稻植株的ABA含量,表明Oshox22调控ABA的生物合成,并可能通过ABA介导的信号传导途径,调控植株对干旱和盐胁迫的应答。玉米HD-Zip I家族Zmhdz10在水稻和拟南芥中的异源表达,增强了转基因植株的耐旱性和耐盐性。在低温处理后,胡萝卜(Daucus carota)根中bZIP类蛋白Lip的表达上调,进而增强了植株的抗寒性。有些植物的bZIP蛋白通过水杨酸、茉莉酸及ABA信号转导通路,参与植株对虫害、病原体感染等生物胁迫的应答调控。目前,盐芥EsbZip60转录因子在调控植物耐逆性中的作用尚无研究报导。
发明内容
本发明的目的在于提供一种调控植物抗氧化能力和耐盐性的盐芥转录因子EsbZip60。
本发明的再一目的在于提供上述盐芥转录因子EsbZip60的编码基因。
本发明的再一目的在于提供含有上述编码基因的重组表达载体。
本发明的再一目的在于提供含有上述编码基因的重组菌株。
本发明的再一目的在于提供上述盐芥转录因子EsbZip60的应用。
本发明的再一目的在于提供上述盐芥转录因子EsbZip60的编码基因的应用。
根据本发明具体实施方式的盐芥转录因子EsbZip60,其氨基酸序列如SEQ IDNo.1所示:
本发明的EsbZip60蛋白由314个氨基酸残基组成,该蛋白包含2个保守的结构域(位于第153-219氨基酸),即靠近C末端的亮氨酸拉链结构域和紧靠亮氨酸拉链N末端的大约20个氨基酸残基组成的碱性结构域。亮氨酸拉链结构域一般可通过疏水作用形成α-螺旋二聚体;碱性结构域可起核定位信号作用,其通过保守的N-x7-R/K区域与特异的DNA结合。另外,EsbZip60转录因子包含富含谷氨酰胺、脯氨酸和一些酸性氨基酸的结构域,其可能与转录激活域相结合从而调控下游基因的表达。
根据本发明具体实施方式的盐芥转录因子EsbZip60基因的基因组序列如SEQ IDNo.2所示:
ATGGCGGAGGAATTTGGTTGCTTTGATATACTTTTAGACGACGATCTCTTCTTCGATTTCGATCCTTCAATCGTAACTGAACCTCCTATTGCGGATGATTTTATTCGCTCTTCACCGGATTCTGCGAATTCGTGGATCGGAGAGATTGAGAGCCAATTGATGAATGATGAGCAGGAGAATTTTCTGGAGTTAGATCAGCAGTCGGTTTCAGAGTTTTTAGCGGATATATTCGTTGATTATCCCACAAGCGAGTCTAGTCCGGTTGAGTTTTCGACCGCTAAAGTTTCAGATGATGTACCAACCGTCGAATCTCCCGCCGCTGGAAAGGAATCAGCAGGATCCGATGATTCCGTGAAGGAGAAAGCAGATTTCTCAATTGAGAAGAAATCGGACGCATCCAATGATTCTGGAAGTGAGAATCAGGATGAAGCTAAGGTGGAAAGCGAGATTGATGGAAACGATGATGCTATGGCCAAGAAAAGAAGAAGGTATATCTAATTTTGTCTCTTTTTAAAGTAATTGAAATTTAGGGTTTGTGCATATTTGGGAAAAGAACATGGTCTGTAATTTGTAAAAGGCGTTATGCTTTAAAAACAACCTTGAGAGCATTATCTTGAAGTAGTTTAGCAGGTGTGTGATTTTGAAATTTTATATTGAGAACAATATGGATTTGTATTTTGGTTGCCTTTTAGATTGTTGAAAGTGTGTTATGCTATATCTCATTGGTTATGTTGTTTAAACATACTTCAAACCAACTTTTACTCTTGTAATTTGTTACTGGTATCTTGTTGAAGTGGTTTGAAGAGTTGATAATGGGTGATTGTGATTGATTTGTTCAGGAGAGTAAGAAACAGAGATGCTGCG GTAAGATCGAGGGAGAGGAAGAAGGAATATGTGACTGATCTAGAGAAGAAGAGTAAGTATCTCGAAAGAGAATGCATGAGACTGGGACGGATGCTCGAGTGTTTCGTTGCGGAAAACCATTCTCTTCGTCTCTGTTTGCAAAAGGGTAGTGGCAATGCTTCCATGATGACAAAGCAGGAGTCTGCTGTGCTCTTGTTGGAATCCCTGCTGTTGGGTTCCCTGCTTTGGTATCTGGGAGACATCATTTGCCAATTCCCTCACCAGCCCCAAACAAAGAGTTGCTTCCAGTCAGTGGAAGCCGGAGAACCAGAAAAGCTGGTTCGAAGCGGGCGAGAGAGTAGTAAAATATCTAATAATAATACCGGGAAGAGTCGGAGATGTAAGGGTTCAAGGCCGCGGATGAAACACCAAGTCTTTACACTTGTGGCGTGA
根据本发明具体实施方式的盐芥转录因子EsbZip60的编码基因,其cDNA核苷酸序列如SEQ ID No.3所示:
根据本发明具体实施方式的重组表达载体,其包括双元农杆菌载体和可用于植物微弹轰击的载体等。所述植物表达载体还可包含外源基因的3’端非翻译区域,即包含聚腺苷酸信号和任何其它参与mRNA加工或基因表达的DNA片段。所述聚腺苷酸信号可引导聚腺苷酸加入到mRNA前体的3’端,如农杆菌冠瘿瘤诱导(Ti)质粒基因(如胭脂合成酶Nos基因)、植物基因3’端转录的非翻译区均具有类似功能。
使用EsbZip60构建重组植物表达载体时,在其转录起始核苷酸前可加上任何一种增强型启动子、组成型启动子或组织特异性启动子,如花椰菜花叶病毒(CaMV)35S启动子、玉米的泛素启动子(Ubiquitin)等,它们可单独使用或与其它植物启动子结合使用;此外,使用本发明的基因构建植物表达载体时,还可使用增强子,包括翻译增强子或转录增强子,这些增强子区域可以是ATG起始密码子或邻接区域起始密码子等,但必需与编码序列的阅读框相同,以保证整个序列的正确翻译。所述翻译控制信号和起始密码子的来源是广泛的,可以是天然的,也可以是合成的。翻译起始区域可以来自转录起始区域或结构基因。
为了便于对转基因植物细胞或植物进行鉴定及筛选,可对所用植物表达载体进行加工,如加入可在植物中表达的编码可产生颜色变化的酶或发光化合物的基因(GUS基因、萤光素酶基因等)、具有抗性的抗生素标记物(庆大霉素标记物、卡那霉素标记物等)或是抗化学试剂标记基因(如抗除莠剂基因)等。从转基因植物的安全性考虑,可不加任何选择性标记基因,直接以逆境筛选转化植株。
利用任何一种可以引导外源基因在植物中表达的载体,将本发明所提供的EsbZip60基因导入植物细胞,可获得提高植物抗逆能力的转基因细胞系及转基因植株。携带有编码基因的表达载体可通过使用Ti质粒、Ri质粒、植物病毒载体、直接DNA转化、显微注射、电导、农杆菌介导等常规生物学方法转化植物细胞或组织,并将转化的植物组织培育成植株。被转化的植物宿主既可以是单子叶植物,也可以是双子叶植物,如:拟南芥、小麦、玉米、黄瓜、番茄、土豆、苜宿等。
本发明还提供上述盐芥转录因子EsbZip60及其编码基因的应用,尤其是在提高植物对盐胁迫的耐逆能力方面的应用。
本发明还提供一种显著提高植物盐胁迫耐受性和抗氧化能力的培育方法,该方法中包括将上述任一种含有EsbZip60基因的重组表达载体导入植物细胞中,即得到对盐胁迫的耐受能力以及抗氧化能力显著提高的植物。
本发明以对盐芥为实验材料,得到EsbZip60蛋白及其编码基因,并将该编码基因导入烟草,显著提升了植物的抗盐和抗氧化的能力。本发明的EsbZip60蛋白及其编码基因对改良、增强转基因植株包括小麦、土豆、黄瓜和番茄等经济作物的耐盐能力,并加速植物抗逆性改良的分子育种进程具有十分重要的理论和实践意义。
附图说明
图1显示EsbZip60转基因烟草的分子检测结果,其中,M:DNAmarker DL2000;1-6:不同转基因烟草株系;N:阴性对照;WT:野生型;P:阳性对照;
图2显示EsbZip60转基因烟草和野生型在含0、50、100、150和200mMNaCl的MS培养基中的生长比较;
图3显示EsbZip60转基因烟草和野生型在0、50、100、150、200mM NaCl处理后的主根长度比较,每组数据20个重复,***表示P<0.001,****表示P<0.0001;
图4显示NaCl处理后EsbZip60转基因烟草和野生型叶和根组织POD酶活性的比较,WT-L:野生型烟草叶组织,T-L:转基因烟草叶组织;WT-R:野生型烟草根组织,T-R:转基因烟草根组织;每组数据3个重复,*表示P<0.05,**表示P<0.01,***表示P<0.001,****表示P<0.0001;
图5显示NaCl处理后EsbZip60转基因烟草和野生型叶组织耐逆相关酶活性的比较,WT-L:野生型烟草叶组织,T-L:转基因烟草叶组织;每组数据3个重复,*表示P<0.05,**表示P<0.01,****表示P<0.0001。
具体实施方式
供试材料为盐芥(Eutrema salsugineum),烟草。收集盐芥、EsbZip60异源表达植株和相应野生型植株的叶和根等用于耐逆相关酶活的定量分析。在所有情况下,叶和根等样品立即于液氮中冷冻并置于-80℃待用,用于提取DNA、RNA或酶液等。
以下实施例中未作具体说明的分子生物学实验方法,均参照《分子克隆实验指南》(第三版)J.萨姆布鲁克一书中所列的具体方法进行,或者按照试剂盒和产品说明书进行。
实施例1盐芥转录因子EsbZip60基因的cDNA克隆
对生长20天左右的盐芥幼苗,用Trizol提取盐芥总RNA。用Superscript II(Invitrogen)反转录酶反转录得到cDNA。根据EsbZip60基因的5’UTR和3’UTR序列设计引物P1和P2。通过反转录得到cDNA为模板,以引物P1和P2进行PCR扩增,引物的序列如下:
P1:5'TTAGATAAAGTCCGATCAGTAG 3'
P2:5'GATTAGAGTAGAAGGCGTGG 3'
对PCR产物进行1%琼脂糖凝胶电泳检测,得到分子量为1056bp的条带,该片段包括EsbZip60的编码区(945bp)和111bp的UTR区域(87bp的5’UTR和24bp的3’-UTR),大小与预期结果相符。
回收该片段,将该回收片段与pMD18-T(Takara)连接,然后转化大肠杆菌DH5α感受态细胞,根据pMD18-T载体上的氨卞青霉素抗性标记筛选阳性克隆,得到含有回收片段的重组质粒。
以该重组质粒载体上的M13F(-47)和M13R(-48)序列为通用引物对其进行核苷酸序列测定,测序结果表明扩增到的EsbZip60基因的开放阅读框(ORF)为SEQ ID No.3的自5’末端第1至945位脱氧核糖核苷酸,编码氨基酸序列是SEQ ID No.1的蛋白质。将含序列SEQID No.3所示EsbZip60基因的重组载体命名为pMD18-T-EsbZip60。
EsbZip60基因进一步用引物P1和P2在盐芥因组中进行扩增,结果显示该基因的基因组序列为1634bp,由两个外显子和一个内含子(351bp)组成。两个外显子长度分别为488bp和457bp。CDS序列为945bp。
实施例2用EsbZip60基因提高植株的抗盐和抗氧化能力
2.1重组表达载体的构建
以盐芥的总RNA反转录得到的cDNA为模板,用含有SmaI和HindIII接头序列的特异引物进行PCR扩增;然后SmaI和HindIII双酶切PCR产物回收,将酶切产物正向插入载体pCAMBIA3301H的35S启动子之后的SmaI和HindIII酶切位点之间,得到重组载体pCAMBIA3301H-35S:EsbZip60。引物序列如下:
35S-EsbZip60[SmaI]:5'TCCCCCGGGTTAGATAAAGTCCGATCAGTAG 3'
35S-EsbZip60[HindIII]:5'CCCAAGCTTGATTAGAGTAGAAGGCGTGG 3'
2.2用EsbZip60基因增强植物的抗盐能力
1)获得转基因烟草材料
将上述构建的重组表达载体pCAMBIA3301H-35S:EsbZip60转化到根癌农杆菌GV3101中,转化烟草,用含6mg/L Basta的MS培养基进行筛选,得到20棵阳性转基因烟草植株。
将筛选得到的阳性转基因植株用PCR做进一步鉴定筛选,PCR所用的一对引物为P1和P2。对35S:EsbZip60转基因烟草进行PCR鉴定,如图1所示,阳性转基因植株经PCR扩增可获得1056bp左右条带,扩增片段中包括945bp的编码区、87bp 5’UTR和24bp 3’-UTR区段,扩增片段大小与预期结果相符,共获得转35S::EsZip60烟草18株。
2)EsbZip60转基因烟草耐盐性及耐逆相关生理指标的测定
野生型和EsbZip60转基因的烟草种子灭菌后,于MS培养基中生长10天,选取生长状况一致的幼苗分别移栽到含0、50、100、150和200mM NaCl的MS培养基上,在光照16h/黑暗8h条件下25℃培养10天;观察表型并进行拍照,同时以每组20株幼苗进行主根长度的测量和统计。
如图2所示,在50、100、150、200mM的NaCl处理10天后,转基因烟草较之于野生型烟草的生长状况更好、主根更长;但在正常条件下(对照)转基因烟草与野生型烟草的生长状况基本一致。
如图3所示,转基因烟草植株在50、100、150、200mM的NaCl浓度下根长平均值均显著大于野生型烟草植株,说明转基因烟草较之于野生型烟草具有更强的耐盐性,表明EsbZip60基因可提高转基因烟草植株的耐盐性。
野生型和EsbZip60转基因烟草生长4周后,选取生长状况一致的幼苗,分别用含0、150和200mM NaCl溶液处理24小时后,采集烟草植株的叶和根,进行其过氧化物酶(POD)活性的测定;每种样品共12棵单株,每4棵为一个重复。结果如图4中的A和图4中的B所示。较之于野生型烟草叶组织,转基因烟草叶分别在150mM、200mM NaCl处理24小时后的POD活性均表现为上调,特别是在150mM NaCl处理后转基因烟草POD酶的活性上调尤为显著,尽管在正常生长条件下,转基因烟草叶组织POD活性低于野生型,如图4中A图所示。较之于野生型烟草根组织,转基因烟草根在正常条件(对照)、150mM、200mM NaCl分别处理24小时后的POD活性均表现为显著上调,如图4中B图所示。
野生型和EsbZip60转基因烟草生长4周后,选取生长状况一致的幼苗,分别用含0、150和200mM NaCl溶液处理48小时后,采集烟草植株的叶和根,进行其过氧化物酶(POD)活性的测定;每种样品共12棵单株,每4棵为一个重复。结果如图4中C图和图4中D图所示。较之于野生型烟草叶组织,转基因烟草叶分别在150mM和200mM NaCl处理48小时后的POD活性均表现为上调,特别是在150mM NaCl处理后转基因烟草POD酶的活性上调尤为显著,但在正常生长条件下,转基因烟草叶组织POD活性低于野生型,如图4中C图所示。较之于野生型烟草根组织,转基因烟草根在正常条件(对照)、150mM和200mM NaCl分别处理48小时后的POD活性均表现为显著上调,如图4中D图所示。
野生型和EsbZip60转基因烟草生长4周后,选取生长状况一致的幼苗,在对照和150mM NaCl处理24小时后采集植株的叶片,进行其超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GPx)和谷胱甘肽还原酶(GR)活性的测定,结果分别如图5中的A、B、C和D图所示。每种样品共12棵单株,每4棵为一个重复。
如图5所示。较之于野生型烟草叶组织,转基因烟草叶在正常条件(对照)的SOD活性表现为上调,在150mM NaCl处理后转基因烟草SOD酶活性表现为显著上调,如图5中A图所示。较之于野生型烟草叶组织,转基因烟草叶在150mM NaCl处理24小时后的CAT活性表现为显著上调,但在正常生长条件下转基因烟草叶组织CAT活性低于野生型,如图5中B图所示。较之于野生型烟草叶组织,转基因烟草叶在150mMNaCl处理24小时后的GPx活性表现为显著上调,但在正常生长条件下转基因烟草叶组织GPx活性低于野生型,如图5中C图所示。较之于野生型烟草叶组织,转基因烟草叶在150mM NaCl处理24小时后的GR活性表现为显著上调,尽管在正常生长条件下转基因烟草叶组织GR活性略低于野生型,如图5中D图所示。
POD和CAT均可清除细胞应激反应中产生的活性氧,在维持氧代谢平衡和减轻活性氧对细胞器的毒害中担任重要作用。而SOD可清除氧自由基(O2 -)对植物细胞的毒害。通常情况下,植物可通过增强体内POD、SOD和CAT的活性以提高植物的抗氧化能力和耐逆性。谷胱甘肽过氧化物酶GSH-Px(GPx)不仅是一种重要的过氧化物分解酶类,也是细胞内抗脂质过氧化酶保护系统的主要成分,其可催化GSH转变为GSSG,并促进H2O2的分解,因而该酶活性可保护细胞膜结构及其功能。谷胱甘肽还原酶(GR)不仅是抗坏血酸-谷胱甘肽循环途径中的重要组分,而且它也是重要的抗氧化酶类,参与植物体内活性氧的清除。鉴于盐芥EsbZip60基因提高了盐胁迫下转基因烟草叶和根中POD酶活性,并提升了盐胁迫下转基因烟草叶中抗氧化酶类SOD、CAT、GPx和GR的酶活性,说明EsbZip60基因提高了转基因烟草植株的抗氧化能力和耐盐性。因而,EsbZip60基因在作物的耐逆基因工程育种中具有一定的应用价值。
序列表
<110> 山东师范大学
<120> 调控植物抗氧化能力和耐盐性的盐芥转录因子EsbZip60及其编码基因和应用
<160> 3
<170> SIPOSequenceListing 1.0
<210> 1
<211> 314
<212> PRT
<213> 盐芥(Eutrema Salsugineum)
<400> 1
Met Ala Glu Glu Phe Gly Cys Phe Asp Ile Leu Leu Asp Asp Asp Leu
1 5 10 15
Phe Phe Asp Phe Asp Pro Ser Ile Val Thr Glu Pro Pro Ile Ala Asp
20 25 30
Asp Phe Ile Arg Ser Ser Pro Asp Ser Ala Asn Ser Trp Ile Gly Glu
35 40 45
Ile Glu Ser Gln Leu Met Asn Asp Glu Gln Glu Asn Phe Leu Glu Leu
50 55 60
Asp Gln Gln Ser Val Ser Glu Phe Leu Ala Asp Ile Phe Val Asp Tyr
65 70 75 80
Pro Thr Ser Glu Ser Ser Pro Val Glu Phe Ser Thr Ala Lys Val Ser
85 90 95
Asp Asp Val Pro Thr Val Glu Ser Pro Ala Ala Gly Lys Glu Ser Ala
100 105 110
Gly Ser Asp Asp Ser Val Lys Glu Lys Ala Asp Phe Ser Ile Glu Lys
115 120 125
Lys Ser Asp Ala Ser Asn Asp Ser Gly Ser Glu Asn Gln Asp Glu Ala
130 135 140
Lys Val Glu Ser Glu Ile Asp Gly Asn Asp Asp Ala Met Ala Lys Lys
145 150 155 160
Arg Arg Arg Arg Val Arg Asn Arg Asp Ala Ala Val Arg Ser Arg Glu
165 170 175
Arg Lys Lys Glu Tyr Val Thr Asp Leu Glu Lys Lys Ser Lys Tyr Leu
180 185 190
Glu Arg Glu Cys Met Arg Leu Gly Arg Met Leu Glu Cys Phe Val Ala
195 200 205
Glu Asn His Ser Leu Arg Leu Cys Leu Gln Lys Gly Ser Gly Asn Ala
210 215 220
Ser Met Met Thr Lys Gln Glu Ser Ala Val Leu Leu Leu Glu Ser Leu
225 230 235 240
Leu Leu Gly Ser Leu Leu Trp Tyr Leu Gly Asp Ile Ile Cys Gln Phe
245 250 255
Pro His Gln Pro Gln Thr Lys Ser Cys Phe Gln Ser Val Glu Ala Gly
260 265 270
Glu Pro Glu Lys Leu Val Arg Ser Gly Arg Glu Ser Ser Lys Ile Ser
275 280 285
Asn Asn Asn Thr Gly Lys Ser Arg Arg Cys Lys Gly Ser Arg Pro Arg
290 295 300
Met Lys His Gln Val Phe Thr Leu Val Ala
305 310
<210> 2
<211> 2204
<212> DNA
<213> 盐芥(Eutrema Salsugineum)
<400> 2
aggcggagga agggcgaaac agacgacgac cccgacgacc caacgaacga accccagcgg 60
agaacgccca ccggacgcga acgggacgga gagagagagc caagagaaga gagcaggaga 120
acggagagac agcagcggca gagagcggaa acggaaccca caagcgagca gccgggagcg 180
accgcaaagc agagagacca accgcgaacc ccgccgcgga aaggaacagc aggaccgaga 240
ccggaaggag aaagcagacc aagagaagaa acggacgcac caagacggaa ggagaacagg 300
agaagcaagg ggaaagcgag agaggaaacg agagcaggcc aagaaaagaa gaaggaacaa 360
gccaaagaag aaaagggggc aagggaaaag aacaggcgaa gaaaaggcga gcaaaaacaa 420
ccgagagcaa cgaagagagc agggggagaa aaagagaaca aaggagaggg ccagaggaaa 480
gggagcaacc aggaggaaac aaccaaacca acaccgaaga cggacggaag gggaagagga 540
aaggggagga gagcaggaga gaagaaacag agagcgcgga agacgaggga gaggaagaag 600
gaaaggacga cagagaagaa gagaagaccg aaagagaagc agagacggga cggagccgag 660
gcggcggaaa accacccgcc ggcaaaaggg agggcaagcc cagagacaaa gcaggagcgc 720
ggccgggaac ccgcggggcc cgcggacggg agacacagcc aaccccacca gccccaaaca 780
aagaggccca gcagggaagc cggagaacca gaaaagcggc gaagcgggcg agagagagaa 840
aaacaaaaaa accgggaaga gcggagagaa gggcaaggcc gcggagaaac accaagcaca 900
cgggcggaat ggcggaggaa tttggttgct ttgatatact tttagacgac gatctcttct 960
tcgatttcga tccttcaatc gtaactgaac ctcctattgc ggatgatttt attcgctctt 1020
caccggattc tgcgaattcg tggatcggag agattgagag ccaattgatg aatgatgagc 1080
aggagaattt tctggagtta gatcagcagt cggtttcaga gtttttagcg gatatattcg 1140
ttgattatcc cacaagcgag tctagtccgg ttgagttttc gaccgctaaa gtttcagatg 1200
atgtaccaac cgtcgaatct cccgccgctg gaaaggaatc agcaggatcc gatgattccg 1260
tgaaggagaa agcagatttc tcaattgaga agaaatcgga cgcatccaat gattctggaa 1320
gtgagaatca ggatgaagct aaggtggaaa gcgagattga tggaaacgat gatgctatgg 1380
ccaagaaaag aagaaggtat atctaatttt gtctcttttt aaagtaattg aaatttaggg 1440
tttgtgcata tttgggaaaa gaacatggtc tgtaatttgt aaaaggcgtt atgctttaaa 1500
aacaaccttg agagcattat cttgaagtag tttagcaggt gtgtgatttt gaaattttat 1560
attgagaaca atatggattt gtattttggt tgccttttag attgttgaaa gtgtgttatg 1620
ctatatctca ttggttatgt tgtttaaaca tacttcaaac caacttttac tcttgtaatt 1680
tgttactggt atcttgttga agtggtttga agagttgata atgggtgatt gtgattgatt 1740
tgttcaggag agtaagaaac agagatgctg cggtaagatc gagggagagg aagaaggaat 1800
atgtgactga tctagagaag aagagtaagt atctcgaaag agaatgcatg agactgggac 1860
ggatgctcga gtgtttcgtt gcggaaaacc attctcttcg tctctgtttg caaaagggta 1920
gtggcaatgc ttccatgatg acaaagcagg agtctgctgt gctcttgttg gaatccctgc 1980
tgttgggttc cctgctttgg tatctgggag acatcatttg ccaattccct caccagcccc 2040
aaacaaagag ttgcttccag tcagtggaag ccggagaacc agaaaagctg gttcgaagcg 2100
ggcgagagag tagtaaaata tctaataata ataccgggaa gagtcggaga tgtaagggtt 2160
caaggccgcg gatgaaacac caagtcttta cacttgtggc gtga 2204
<210> 3
<211> 945
<212> DNA
<213> 盐芥(Eutrema Salsugineum)
<400> 3
atggcggagg aatttggttg ctttgatata cttttagacg acgatctctt cttcgatttc 60
gatccttcaa tcgtaactga acctcctatt gcggatgatt ttattcgctc ttcaccggat 120
tctgcgaatt cgtggatcgg agagattgag agccaattga tgaatgatga gcaggagaat 180
tttctggagt tagatcagca gtcggtttca gagtttttag cggatatatt cgttgattat 240
cccacaagcg agtctagtcc ggttgagttt tcgaccgcta aagtttcaga tgatgtacca 300
accgtcgaat ctcccgccgc tggaaaggaa tcagcaggat ccgatgattc cgtgaaggag 360
aaagcagatt tctcaattga gaagaaatcg gacgcatcca atgattctgg aagtgagaat 420
caggatgaag ctaaggtgga aagcgagatt gatggaaacg atgatgctat ggccaagaaa 480
agaagaagga gagtaagaaa cagagatgct gcggtaagat cgagggagag gaagaaggaa 540
tatgtgactg atctagagaa gaagagtaag tatctcgaaa gagaatgcat gagactggga 600
cggatgctcg agtgtttcgt tgcggaaaac cattctcttc gtctctgttt gcaaaagggt 660
agtggcaatg cttccatgat gacaaagcag gagtctgctg tgctcttgtt ggaatccctg 720
ctgttgggtt ccctgctttg gtatctggga gacatcattt gccaattccc tcaccagccc 780
caaacaaaga gttgcttcca gtcagtggaa gccggagaac cagaaaagct ggttcgaagc 840
gggcgagaga gtagtaaaat atctaataat aataccggga agagtcggag atgtaagggt 900
tcaaggccgc ggatgaaaca ccaagtcttt acacttgtgg cgtga 945
Claims (9)
1.调控植物抗氧化能力和耐盐性的盐芥转录因子EsbZip60,其特征在于,其氨基酸序列如SEQ ID No.1所示。
2.盐芥转录因子EsbZip60编码基因,其特征在于,其编码权利要求1所述的、调控植物抗氧化能力和耐盐性的盐芥转录因子EsbZip60。
3.根据权利要求2所述的盐芥转录因子EsbZip60编码基因,其特征在于,其核苷酸序列如SEQ ID No.2或SEQ ID No.3所示。
4.包含权利要求2所述的盐芥转录因子EsbZip60编码基因的重组表达载体。
5.包含权利要求2所述的盐芥转录因子EsbZip60编码基因的重组菌株。
6.权利要求1所述的调控植物抗氧化能力和耐盐性的盐芥转录因子EsbZip60的应用。
7.权利要1所述的调控植物抗氧化能力和耐盐性的盐芥转录因子在提高植物的耐盐能力和抗氧化性方面的应用。
8.权利要求2所述的盐芥转录因子EsbZip60编码基因的应用。
9.一种提高植物的抗氧化能力和耐盐性的方法,其特征在于,所述方法包括在植物中表达权利要求2所述盐芥转录因子EsbZip60编码基因的步骤。
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202010193833.XA CN111423499B (zh) | 2020-03-19 | 2020-03-19 | 调控植物抗氧化能力和耐盐性的盐芥转录因子EsbZip60及其编码基因和应用 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202010193833.XA CN111423499B (zh) | 2020-03-19 | 2020-03-19 | 调控植物抗氧化能力和耐盐性的盐芥转录因子EsbZip60及其编码基因和应用 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN111423499A true CN111423499A (zh) | 2020-07-17 |
CN111423499B CN111423499B (zh) | 2022-02-25 |
Family
ID=71548080
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202010193833.XA Expired - Fee Related CN111423499B (zh) | 2020-03-19 | 2020-03-19 | 调控植物抗氧化能力和耐盐性的盐芥转录因子EsbZip60及其编码基因和应用 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN111423499B (zh) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112626086A (zh) * | 2021-01-20 | 2021-04-09 | 山东大学 | 蒺藜苜蓿基因MtREVOLUTA在提高豆科近缘饲草紫花苜蓿耐盐性中的应用 |
CN114214334A (zh) * | 2022-01-12 | 2022-03-22 | 山东农业大学 | 来源于盐芥的基因EsH2A.3在调控植物耐盐性中的应用 |
CN116121262A (zh) * | 2022-12-07 | 2023-05-16 | 青岛农业大学 | 一种基于苦荞麦FtIST1基因调控植物耐盐性的应用方法 |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103114091A (zh) * | 2013-02-22 | 2013-05-22 | 山东师范大学 | 一种与植物耐盐性相关的miRNA及其应用 |
-
2020
- 2020-03-19 CN CN202010193833.XA patent/CN111423499B/zh not_active Expired - Fee Related
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103114091A (zh) * | 2013-02-22 | 2013-05-22 | 山东师范大学 | 一种与植物耐盐性相关的miRNA及其应用 |
Non-Patent Citations (4)
Title |
---|
HENRIQUEZ-VALENCIA C等: "bZIP17 and bZIP60 Regulate the Expression of BiP3 and Other Salt Stress Responsive Genes in an UPR-Independent Manner in Arabidopsis thaliana", 《JOURNAL OF CELLULAR BIOCHEMISTRY》 * |
TANG W等: "Elevated tolerance to salt stress in transgenic cells expressing transcription factor AtbZIP60 is associated with the increased activities of H+-ATPase and acid phosphatase", 《PLANT BIOTECHNOLOGY REPORTS》 * |
TANG W等: "Overexpression of AtbZIP60deltaC Gene Alleviates Salt-induced Oxidative Damage in Transgenic Cell Cultures", 《PLANT MOLECULAR BIOLOGY REPORTER》 * |
TANG W等: "Transcription factor AtbZIP60 regulates expression of Ca2+-dependent protein kinase genes in transgenic cells", 《MOLECULAR BIOLOGY REPORTS》 * |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112626086A (zh) * | 2021-01-20 | 2021-04-09 | 山东大学 | 蒺藜苜蓿基因MtREVOLUTA在提高豆科近缘饲草紫花苜蓿耐盐性中的应用 |
CN114214334A (zh) * | 2022-01-12 | 2022-03-22 | 山东农业大学 | 来源于盐芥的基因EsH2A.3在调控植物耐盐性中的应用 |
CN114214334B (zh) * | 2022-01-12 | 2023-08-04 | 山东农业大学 | 来源于盐芥的基因EsH2A.3在调控植物耐盐性中的应用 |
CN116121262A (zh) * | 2022-12-07 | 2023-05-16 | 青岛农业大学 | 一种基于苦荞麦FtIST1基因调控植物耐盐性的应用方法 |
Also Published As
Publication number | Publication date |
---|---|
CN111423499B (zh) | 2022-02-25 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN111423499B (zh) | 调控植物抗氧化能力和耐盐性的盐芥转录因子EsbZip60及其编码基因和应用 | |
CN110066326B (zh) | 调控植物花青素合成的盐芥转录因子EsMYB41及其编码基因和应用 | |
CN107188940B (zh) | GsHA12蛋白及其编码基因在调控植物耐逆性中的应用 | |
CN109666659B (zh) | 植物抗旱、耐盐蛋白AsCIPK14及其编码基因和应用 | |
CN111171125B (zh) | 蛋白质IbCAF1在调控植物耐盐抗旱性中的应用 | |
CN109666681B (zh) | 植物抗旱、耐盐蛋白EeCIPK26及其编码基因和应用 | |
CN111153979B (zh) | 抗旱相关蛋白IbBT4及其编码基因与应用 | |
CN111153975B (zh) | 植物抗旱相关蛋白TaNAC15及其编码基因与应用 | |
CN113563442B (zh) | 抗旱相关蛋白IbSPB1及其编码基因与应用 | |
Wang et al. | Over-expressing GsGST14 from Glycine soja enhances alkaline tolerance of transgenic Medicago sativa | |
Li et al. | Overexpression of an ABA-inducible homeodomain-leucine zipper I gene MsHB7 confers salt stress sensitivity to alfalfa | |
CN111218470A (zh) | 一种调控植物抗逆性的方法 | |
CN107602683B (zh) | 一个来源于玉米的转录因子ZmNLP4及其用途 | |
CN110964091B (zh) | 小麦抗旱杂种优势相关蛋白TaNF-YB12及其编码基因和应用 | |
CN110295175B (zh) | 一个大豆NAC转录因子家族基因Glyma08g41995的应用 | |
CN110684088B (zh) | 蛋白ZmbZIPa3及其编码基因在调控植物生长发育与耐逆性中的应用 | |
CN114525298B (zh) | 大豆蛋白GmFVE在植物耐盐性调控中的应用 | |
CN111808180B (zh) | 植物抗旱杂种优势相关蛋白TaNF-YB3及其编码基因和应用 | |
CN114591409A (zh) | TaDTG6蛋白在提高植物抗旱性中的应用 | |
CN109734786B (zh) | 植物花粉育性恢复相关蛋白TaDMT25及其编码基因和应用 | |
CN112094331A (zh) | 一种植物抗旱杂种优势相关蛋白TaATG5及其编码基因和应用 | |
CN111235125B (zh) | 植物耐盐性、抗氧化和抗真菌能力相关硫氰酸酶EsSTR4A及其编码基因和应用 | |
CN113563443B (zh) | 耐盐相关蛋白IbWRKY32及其编码基因与应用 | |
CN109777790B (zh) | 植物抗旱、耐盐相关蛋白EeSAPK4及其编码基因和应用 | |
CN111303260B (zh) | 一种植物抗逆性相关蛋白OsC3HC4及编码基因与应用 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20220225 |
|
CF01 | Termination of patent right due to non-payment of annual fee |