CN111334449A

CN111334449A - Sludge reduction microbial agent, and preparation method and use method thereof

Info

Publication number: CN111334449A
Application number: CN201811552094.8A
Authority: CN
Inventors: 朱臻
Original assignee: Yangzhou Zhenwei Biotechnology Co ltd
Current assignee: Yangzhou Zhenwei Biotechnology Co ltd
Priority date: 2018-12-19
Filing date: 2018-12-19
Publication date: 2020-06-26

Abstract

The invention relates to the field of sewage treatment, in particular to a sludge reduction microbial agent, and a preparation method and a use method thereof. Provides a sludge reduction microbial agent which does not affect the quality of effluent water and can adapt to various sewage biochemical treatment systems and has good impact resistance of floras to sewage, a preparation method and a use method thereof. The sludge reduction microbial agent comprises the following components in parts by mass: 26-34 parts of lactic acid bacteria, 17-23 parts of yeast, 3-6 parts of acetic acid bacteria, 8-13 parts of photosynthetic bacteria, 7-12 parts of actinomycetes, 8-12 parts of archaea and 12-18 parts of bacillus. The invention has strong impact resistance, can reduce the sludge amount by more than 50 percent and ensure the effluent quality.

Description

Sludge reduction microbial agent, and preparation method and use method thereof

Technical Field

The invention relates to the field of sewage treatment, in particular to a sludge reduction microbial agent, and a preparation method and a use method thereof.

Background

Along with the aggravation of human activities, a large amount of various domestic sewage and industrial sewage are generated, people utilize an activated sludge method to widely apply sewage treatment, excess sludge is generated as a byproduct of a sewage treatment plant in a large amount, 80% of the excess sludge in China is not properly treated at present, and serious secondary pollution is generated to the natural environment.

The current sludge reduction main technologies are as follows: ozone oxidation, ultrasonic cell disruption, uncoupling, chemical methods, etc., but the cost is too high; the microbiological method starts with sewage treatment which is the source of sludge generation, and can effectively solve the problem of reducing the sludge generation amount in the treatment of industrial wastewater and urban domestic sewage while treating sewage under the condition of not changing the original sewage treatment equipment, process and method.

After the concentration of the sludge in a normal sewage treatment system is increased, the dissolved oxygen is low, so that the properties of the sludge are changed, the sludge is loose, the quality of effluent is poor, and the effluent is turbid.

The activated sludge mainly comprises microorganisms with biological activity and carriers thereof, aged and dead microorganisms, inert organic matters and partial inorganic matters brought into raw water. After the sludge concentration is improved, the sludge age is improved, the amount of aged and dead sludge in the system is increased, the sludge settleability is deteriorated, the biochemical effect is reduced, and the phenomena of sludge turning over and exceeding standard of effluent can be caused seriously. Generally, the problem can be solved only by increasing the discharge of the excess sludge, but some raw water has large water quality fluctuation, some high-salt wastewater, high-ammonia nitrogen wastewater and high-phenol toxic wastewater suddenly enter a system, and after a large amount of sludge is discharged, the system is easily impacted, even the system is impacted and crashed, so that the original basic reaction cannot be carried out, the sewage cannot be treated, and the sludge amount is reduced. For example, patent No. 201210265567.2 discloses a composite microbial agent for sludge reduction, its preparation method and application, which utilizes the interaction between microbial groups to form a biodegradation system with high activity of various enzymes, effectively destroys and decomposes dead and aged bacteria in activated sludge, and can decompose organic substances, and reduce the generation amount of excess sludge without affecting the quality of effluent; however, the flora can not adapt to variable sewage components, the impact resistance to the sewage is poor, the application cost is high, and the effect is not ideal.

Disclosure of Invention

Aiming at the problems, the invention provides a sludge reduction microbial agent which does not affect the quality of effluent and is suitable for various sewage biochemical treatment systems and good in impact resistance of the microbial community on sewage, and a preparation method and a use method thereof.

The technical scheme of the invention is as follows:

the sludge reduction microbial agent comprises the following components in parts by mass: 26-34 parts of lactic acid bacteria, 17-23 parts of yeast, 3-6 parts of acetic acid bacteria, 8-13 parts of photosynthetic bacteria, 7-12 parts of actinomycetes, 8-12 parts of archaea and 12-18 parts of bacillus.

A preparation method of a sludge reduction microbial agent comprises the following steps:

1) and strain activation: respectively inoculating nonpathogenic lactobacillus, saccharomycetes, acetic acid bacteria, photosynthetic bacteria, actinomycetes, archaebacteria and bacillus into respective test tube slant culture media, culturing for 2-5 days in an incubator at the temperature of 28-37 ℃, and storing in a refrigerated cabinet at the temperature of 4-8 ℃ for later use after the culture is finished;

2) and preparing a triangular flask culture medium: preparing culture media corresponding to all strains according to a proportion, putting the culture media into a triangular flask, sterilizing by using a culture medium sterilization cabinet at the sterilization temperature of 118-121 ℃ and the sterilization pressure of 95-115 KP, and putting the culture media into a sterile room to cool to normal temperature after sterilization is finished;

3) and first-stage culture: respectively inoculating the strains obtained in the step 1) into the corresponding triangular flask culture medium obtained in the step 2) in an aseptic environment, and performing shaking culture for 48 hours at the culture temperature of 28-37 ℃ to obtain a primary culture solution of each strain;

4) and secondary culture: taking the primary culture solution of each strain as a seed solution, inoculating the seed solution into respective triangular flask culture medium again, and carrying out shake culture for 48 hours at the culture temperature of 28-37 ℃ to obtain a secondary culture solution of each strain;

5) and (3) expanding culture:

5.1), aerobic fermentation: inoculating the secondary culture solution of each strain into an amplification culture medium according to a ratio of 0.8-1.2: 9, then placing the mixture into a fermentation tank, introducing sterile air to control the dissolved oxygen to be 7mg/L, and fermenting for 0.5-2 hours;

5.2) anaerobic fermentation: and (3) after aerobic fermentation, the introduction of sterile air is canceled, and sealed fermentation is carried out for 34-35.5 hours to obtain the sludge reduction microbial agent.

The expanding culture medium in the step 5) comprises, by mass, 0.8-1.2 parts of rice vinegar, 0.9-1.1 parts of brown sugar, 0.8-1.2 parts of 40% edible alcohol and 55-65 parts of water.

And 5) controlling the fermentation heat by using a water circulation heat exchange system in the fermentation process to keep the fermentation temperature at 37 +/-1 ℃.

A use method of a sludge reduction microbial agent comprises the following steps:

s1, normal monthly dosage:

according to the daily treated water quantity of the system, indexes such as the water quality of raw water, system design parameters, sludge load and the like, and process design characteristics of a combined device, combined with design and use experience, the urban sewage is generally controlled to be 0.005-0.01 percent, the chemical sewage is generally controlled to be 0.01-0.04 percent, and the adding amount (the water fluctuation is less than or equal to 10 percent) is determined according to the adding proportion and the daily treated water quantity; for example, after a municipal sewage treatment plant with 10 ten thousand of water treated per day operates normally, the normal dosage per month is 5-10 tons.

S2, adding:

adding the sludge reduction microbial agent which is 6-8 times of the adding amount determined in the step S1 into the water inlet or the sludge return port of the aerobic tank, and supplementing and adding the microbial agent according to the adding amount determined in the step S1 every month.

The supplementary feeding in the step S2 is divided into 1-2 times.

The invention has the beneficial effects that:

1. strong impact resistance

Through the adding use of PM-1 sludge decrement microbial preparation, as PM-1 is rich in various active thalli, after entering a system, through the growth and the propagation of the PM-1 sludge decrement microbial preparation, the total amount of flora in the system can be increased, the general class of flora in the system is also increased, so that the number and the class of system microbes can be improved, such as extreme salt-tolerant bacteria, phosphorus-accumulating bacteria and the like, the high-salinity wastewater can be tolerated, the salt concentration of the wastewater can be quickly reduced, various toxic substances such as phenol, chloroaniline and the like contained in the wastewater can be quickly degraded, the photosynthetic flora has better tolerance and decomposition effects on the high ammonia nitrogen and high organic phosphorus wastewater, the adaptability of the system to raw water is increased, and the impact resistance of the system is improved.

Meanwhile, after the PM-1 microbial agent is added, the total amount of microorganisms in the same system is improved after the sludge concentration of the system is properly improved, the adsorption capacity of the sludge on organic pollutants is further enhanced after the sludge concentration is improved, and the buffer time of the system can be prolonged after the adsorption capacity of the pollutants in water which is abnormally impacted is improved, so that the impact resistance of the system is improved, and the impact resistance of the system is reduced.

2. The sludge amount can be reduced by more than 50 percent

By adding the microbial agent, the microbial active flora in the sludge can be strengthened, the biological activity of the flora can be enhanced, the sludge age can be prolonged, and the sludge concentration can be further improved through the metabolism of the active flora. The sludge age can be prolonged, the proportion of high-activity microorganisms in the sludge can be increased, and organic matters in the sludge are used as substrates to participate in metabolism, so that the sludge yield is reduced; the sludge concentration is increased, more organic matters can be consumed through endogenous respiration, and therefore the sludge yield is reduced;

3. guarantee of effluent quality

(1) Organic pollutants are decomposed in a mode of 'co-metabolism degradation' and 'oxidative decomposition', so that BOD (biochemical oxygen demand) and COD (chemical oxygen demand) in the sewage are reduced;

(2) containing nitrobacteria (nitrifying bacillus vickers) and denitrifying bacteria (denitrifying bacillus denitrificans) (neither the nitrifying bacteria nor the denitrifying bacteria are mentioned in the context of the method, so that the intermediate products are N in the ammonia nitrogen oxidation process₂、N₂O, NO escape in a gas form, so that the ammonia nitrogen content of the effluent is reduced, and the ammonia nitrogen of the effluent is more stable;

(3) the phosphorus-accumulating bacteria such as acinetobacter and pseudounicellular bacteria can occupy 25-33% of dry cell weight after pure strain intensive culture, form a phosphorus reservoir in a cell body, do not release phosphorus under a short-time anaerobic condition, do not influence the removal of phosphorus under the condition of discharging about 50% less sludge, and can improve the removal rate of phosphorus if the sludge reduction rate is properly reduced.

Detailed Description

The lactic acid bacteria comprise Lactococcus lactis (Lactococcus lactis), streptococcus lactis (Streptococcus lactis) and bifidobacteria (Bifidobacterium Bifidum), a large amount of lactic acid generated by the lactic acid bacteria can carry out biological detoxification in an aerobic system and digest toxic and harmful substances contained in the sewage, and the generated antibacterial peptides such as lactobacillin and Bifidum can effectively inhibit the propagation of disease and mixed bacteria in the sewage and promote the propagation of beneficial bacteria into dominant bacteria; stimulates the activity of macrophages, and increases the phagocytic capacity of the macrophages on aged dead bacteria.

The yeast mainly comprises Candida, Candida albicans, Cryptococcus albicans, Rhodotorula rubra, and Debaryomyces hansenii. The saccharomycetes can synthesize various proteins and amino acids by utilizing carbohydrate contained in the sewage, and provide easily absorbed nutrient substances for other beneficial flora; the metabolism of the yeast can generate vitamin B1, B2 and nicotinic acid, and secrete a large amount of extracellular hydrolase, thereby playing a good role in promoting the degradation of organic matters in the sewage.

Acetobacter Acetobacter is mainly Acetobacter oxyacantha (Acetobacter oxydans), Acetobacter pasteurianus (Acetobacter pasteurianus) and Acetomonas Acetobacter oxyacantha (Acetomonas oxydans). The acetic acid synthesized by the acetic acid bacteria can inhibit the propagation of disease bacteria, and is fermented together with the yeast to greatly promote the propagation of the yeast.

The main strains of the actinomycetes: streptomyces (Streptomyces), Micromonospora (Micromonospra), Nocardia (Nocard's bacillus) and Actinoplanate (Actinoplananet) actinomycetes can decompose a plurality of organic substances, including complex compounds such as aromatic compounds, paraffin, rubber, cellulose and wood and some compounds with strong toxicity such as cyanogen. In the metabolic process, antibiotic substances are generated, and the stabilizing effect on the structures of all microbial populations in the microbial inoculum is achieved.

The photosynthetic bacteria include Rhodospirillum rubrum, Rhodopseudomonas aeruginosa, and Rhodopseudomonas sphaeroides. The photosynthetic bacteria have strong decomposition capability on organic substances under the conditions of light anaerobic and dark aerobic, and have better tolerance and decomposition capability on toxic and harmful substances such as cyanogen, benzene and the like.

Archaea mainly comprises halobacter (H.salinium), and a complex microbial flora fermented by adding halotolerant bacteria has better tolerance and adaptability under different environmental conditions.

The Bacillus group mainly comprises Bacillus subtilis, Bacillus licheniformis, Bacillus cereus and Bacillus alcalophilus. The flora has strong degradation function on organic substances and secretes a large amount of protease, amylase, lipase, cellulase and the like; the produced chitinase can dissolve the cell wall of disease fungi, kill the fungi and promote the benign growth of aquatic microbial population, and the alkalophilic bacillus has the functions of removing nitrogen and sulfur and resisting salinity.

The culture medium corresponding to each strain is as follows:

(1) lactic acid bacteria

Culture medium (pH 7.0)	Proportioning (parts by weight)
		Casein peptone	10.0g
Beef extract	10.0g
		Yeast extract	5.0g
Glucose	5.0g
		Sodium acetate	5.0g
Diammonium hydrogen citrate	2.0g
		Tween 80	1.0g
K₂HPO₄	2.0g
		MgSO₄ . 7H₂O	0.2g
MnSO₄. H₂O	0.05g
		CaCO₃	20.0g
Distilled water	1.0L

(2) Yeast:

6- - -8Brix. wort 1.0 liter pH: nature of nature

(3) Acetic acid bacteria: glucose 8g, yeast powder 10g, distilled water 1L PH5.5

Sterilizing at 0.1mpa pressure for 20 min, adding 40ml of 95% edible alcohol

(4) Actinomycetes:

culture medium (pH 7.2-7.4)	Proportioning (parts by weight)
		Soluble starch	20.0
KNO₃	1.0
		K₂HPO₄	0.5
MgSO₄.7H₂O	0.01
		NaCL	0.5
FeSO_4.7H₂O	0.01
		Water (W)	1000.0

(5) Photosynthetic bacteria: a:

culture medium (pH 7.0)	Proportioning (parts by weight)
		NH₄Cl	1g
K₂HPO₄	0.5g
		MgCl₂	0.2g
NaCl	2.0g
		Yeast cream	0.1g
Distilled water	1.0L

b 5.0 g NaHCO₃Dissolving in 50 ml of distilled water

c, absolute ethyl alcohol 2.0 g

d:0.1N.H₃PO₄

Note: respectively filtering b, C and d, sterilizing, mixing a, b and C, adjusting pH to 7.0 with d, sterilizing at 121 deg.C for 15 min

(6) Archaea:

culture medium (pH 7.2)	Proportioning (parts by weight)
		Hydrolyzed milk protein	7.5g
Yeast extract	10.0g
		Citric acid sodium salt	3.0g
MgSO₄·7H₂O	20.0g
		KCl	2.0g
FeSO₄·7H₂O	10×10¯ ⁶g
		NaCl	200.0g
Agar-agar	15.0g
		Distilled water	1.0L

(7) Bacillus:

culture medium (pH 7.0)	Proportioning (parts by weight)
		Peptone	10g
Beef extract	3g
		NaCl	5g
Agar-agar	15g
		Distilled water	1.0L

The action mechanism of the invention is as follows:

1. sludge reduction

(1) Bioaugmentation

Not all of the microorganisms of the sewage treatment biochemical system are the most effective microorganisms. The microbial agent of the invention is a specially selected microbial strain, and the selected put strain can maintain and strengthen the activity of the naturally occurring strain, thereby optimizing and controlling the balance of microbial population. In addition, the invention can increase the variety, concentration and metabolic activity of bacteria in the system, inhibit the growth of a small amount of unfavorable bacteria, enhance the sludge activity in the system, optimize the biochemical treatment effect and improve the shock resistance of the system.

(2) Biological wall breaking

Bacterial lysozymes are three broad classes, N-acetylhexosaminidases, which catalyze the hydrolysis of β (1 → 4) glycosidic linkages in the carbohydrate backbone of peptidoglycans, N-acetylmuramyl-alanine amidases, which catalyze the cleavage of peptidyl and glycosyl groups in peptidoglycans, and endopeptidases, which catalyze the cleavage of peptide bonds in peptidoglycan peptide bridges.

The bacterial lysozyme attacks β -1, 4-glycosidic bonds between N-acetylglucosamine and N-acetylmuramic acid in glycan chains to break the glycosidic bonds, so that the bacterial cell walls are disintegrated.

(3) Biodegradation

The sewage treatment is to decompose organic matters into available small molecular components by utilizing natural microbial populations. One part of which synthesizes microbial cells (which, through metabolism, utilize small molecules in wastewater treatment to synthesize substances required for their own ability), and the other part of which is oxidized to carbon dioxide and water to obtain energy, for example 1000g of nutrients, 600g of which is used to synthesize microorganisms and 400g of which is oxidized to CO₂And H₂And O, thereby obtaining energy. This indicates that 40% of organic matter can be reduced during the growth phase of bacteria, and when the organic matter in the organism can also be used as the substrate of microorganism and the above metabolism is repeated, the yield of sludge is reduced, and the purpose of sludge reduction is achieved.

The key point of biological degradation of organisms lies in the dissolution of microbial cells, after the microbial agent disclosed by the invention is added, microorganisms can generate a large amount of hydrolytic extracellular enzymes for dissolving microbial cell walls, as the living cells generate extracellular polysaccharide EPS (extracellular polysaccharide of lactic acid bacteria), the cells are protected, and lysozyme only aims at dead or aged cells and cannot influence the cells with high activity. The reason why the quality of the effluent is not influenced by adding the microbial agent is also the reason.

(4) Biological digestion

After the microbial agent is added, the sludge age can be prolonged, the sludge concentration is improved, and the number of effective microorganisms in the sludge is increased, so that the sludge load is reduced; meanwhile, due to the prolonging of the sludge age, the proportion of microorganisms with high activity in the sludge is increased, and the biochemical treatment effect is enhanced.

The sludge concentration is improved, more organic matters can be consumed through endogenous respiration, the sludge production is reduced, the sludge discharge is correspondingly reduced, and the purpose of sludge reduction is finally achieved.

(5) Biological predation

Energy is lost during the transfer from low to high nutritional levels due to inefficient bioconversion, and therefore, ideally, the total amount of energy lost is maximized and the biomass produced is minimized, i.e., the longer the food chain, the greater the energy loss, and the less energy is used to synthesize the biomass. One way of reducing biomass is therefore to greatly promote the biological growth of prey bacteria in the food chain, according to biological principles.

Common predators are protozoa and metazoa, which are more or less present in conventional active systems, but since the growth cycle of these organisms is much longer than that of microorganisms, the amount of protozoa and metazoa is very small if the sludge is too short. The microbial agent has no great effect, and due to the comprehensive effect generated after the microbial agent is added, the sludge age is greatly prolonged, a large amount of original organisms and after organisms exist, the biological predation effect is obvious, the sludge production amount is reduced, and the purpose of sludge reduction is achieved.

2. Guarantee of water quality

(1) Guarantee the COD and BOD of the effluent

Some compounds are not utilized by microorganisms as a sole carbon or energy source for organic matter in wastewater, and when other compounds capable of utilizing carbon or energy sources are present, such compounds can be degraded simultaneously in a manner known as "Co-metabolism" (Co-oxidation) or "Co-oxidation". After the microbial agent is added, the co-metabolism degradation mode plays an important role in thoroughly decomposing organic pollutants, particularly some pollutants which are difficult to degrade, and can oxidize and decompose the organic pollutants, thereby being more beneficial to removing COD and BOD.

(2) Guarantee of ammonia nitrogen and total nitrogen in effluent

For the guarantee of the ammonia nitrogen in the effluent, three aspects of modes are provided:

firstly, the sludge age is doubled without changing the sludge concentration due to the 50% reduction of the sludge yield. The growth of the sludge age is more favorable for the growth of the nitrifying bacteria due to the longer generation period of the nitrifying bacteria, and the proportion of the nitrifying bacteria in the activated sludge is more reasonable;

secondly, the microbial agent is a compound bacterium, and the preparation contains special nitrobacteria and denitrifying bacteria, so that the quantity of the nitrobacteria and the denitrifying bacteria is artificially increased;

through more nitrifying bacteria and denitrifying bacteria and better bacteria and denitrifying conditions, the ammonia nitrogen in the effluent is more stable.

Thirdly, the microbial agent of the invention contains full-course nitrifying bacteria (also called aerobic denitrifying bacteria), the full-course nitrification is completed under the action of microorganisms, and three intermediate products N are generated in the process of oxidizing ammonia nitrogen into nitrate₂、N₂O, NO will escape as a gas, leading to loss of total nitrogen and removal of the total nitrogen. For sewage treatment plants, total nitrogen can be removed without denitrification, relieving the pressure of denitrification.

(3) Guarantee of total phosphorus in effluent

For general microorganisms in activated sludge, the phosphorus content is 1.5-1.7% of the dry weight of the cells; for phosphorus accumulating bacteria in the activated sludge, the phosphorus content only accounts for 3.3-13% of the dry weight of cells, and phosphorus is released under the anaerobic condition; the phosphorus-accumulating bacteria in the microbial agent of the invention are as follows: after the pure strain is intensively cultured, phosphorus can account for 25-33% of the dry weight of cells, a phosphorus pool is formed in a cell body, and phosphorus is not released under the anaerobic condition in a short time.

Therefore, the phosphorus removal is not affected when about 50% of sludge is discharged. If the sludge reduction rate is appropriately reduced, the phosphorus removal rate can also be increased.

In the test process, phosphorus can be used as a research object, and the reduction rate of the sludge is reflected by the material balance of the phosphorus.

The present invention will be further described with reference to the following examples.

Example 1

The sludge reduction microbial agent comprises the following components in parts by mass: 26 parts of lactic acid bacteria, 17 parts of saccharomycetes, 3 parts of acetic acid bacteria, 8 parts of photosynthetic bacteria, 7 parts of actinomycetes, 8 parts of archaea and 12 parts of bacillus.

The preparation of the microbial inoculum comprises the following steps:

1) and strain activation: respectively inoculating nonpathogenic lactobacillus, yeast, acetic acid bacteria, photosynthetic bacteria, actinomycetes, archaebacteria and bacillus into respective test tube slant culture medium, culturing in an incubator at 28 deg.C for 2 days, and storing in a refrigerated cabinet at 4 deg.C for use;

2) and preparing a triangular flask culture medium: preparing culture medium corresponding to each strain according to a certain proportion, placing the culture medium into a triangular flask, sterilizing by using a culture medium sterilization cabinet at the sterilization temperature of 118 ℃ and the sterilization pressure of 95KP, and placing the culture medium into a sterile room to cool to normal temperature after the sterilization is finished;

3) and first-stage culture: respectively inoculating the strains obtained in the step 1) into the corresponding triangular flask culture medium obtained in the step 2) in an aseptic environment, and performing shaking culture for 48 hours at the culture temperature of 28 ℃ to obtain a primary culture solution of each strain;

4) and secondary culture: taking the primary culture solution of each strain as a seed solution, inoculating into respective triangular flask culture medium again, and performing shake culture for 48 hours at the culture temperature of 28 ℃ to obtain a secondary culture solution of each strain;

5) and (3) expanding culture:

5.1), aerobic fermentation: inoculating the secondary culture solution of each strain into an amplification culture medium according to the proportion, wherein the proportion of the secondary culture solution to the amplification culture medium is 0.8: 9, then placing the mixture into a fermentation tank, introducing sterile air to control the dissolved oxygen to be 7mg/L, and fermenting for 0.5 hour;

5.2) anaerobic fermentation: and (3) after aerobic fermentation, the introduction of sterile air is canceled, and sealed fermentation is carried out for 34 hours to obtain the sludge reduction microbial agent.

The expanding culture medium in the step 5) comprises 0.8 part of rice vinegar (the concentration of total acid is more than 5%), 0.9 part of brown sugar, 0.8 part of amino acid peptide chelating agent and 55 parts of water by mass.

And 5) controlling the fermentation heat by using a water circulation heat and cold exchange system in the fermentation process to keep the fermentation temperature at 37 ℃.

Example 2

The sludge reduction microbial agent comprises the following components in parts by mass: 34 parts of lactic acid bacteria, 23 parts of yeast, 6 parts of acetic acid bacteria, 13 parts of photosynthetic bacteria, 12 parts of actinomycetes, 12 parts of archaea and 18 parts of bacillus.

The preparation method of the sludge reduction microbial agent comprises the following steps:

1) and strain activation: respectively inoculating nonpathogenic lactobacillus, yeast, acetic acid bacteria, photosynthetic bacteria, actinomycetes, archaebacteria and bacillus into respective test tube slant culture medium, culturing for 5 days in an incubator at 37 ℃, and storing in a refrigerated cabinet at 8 ℃ for later use after the culture is finished;

2) and preparing a triangular flask culture medium: preparing culture media corresponding to each strain according to a proportion, placing the culture media into a triangular flask, sterilizing by using a culture medium sterilization cabinet at the sterilization temperature of 121 ℃ and the sterilization pressure of 115KP, and placing the culture media into a sterile room to cool to normal temperature after the sterilization is finished;

3) and first-stage culture: respectively inoculating the strains obtained in the step 1) into the corresponding triangular flask culture medium obtained in the step 2) in an aseptic environment, and performing shaking culture for 48 hours at the culture temperature of 37 ℃ to obtain a primary culture solution of each strain;

4) and secondary culture: taking the primary culture solution of each strain as a seed solution, inoculating into respective triangular flask culture medium again, and performing shake culture for 48 hours at a culture temperature of 37 ℃ to obtain a secondary culture solution of each strain;

5) and (3) expanding culture:

5.1), aerobic fermentation: inoculating the secondary culture solution of each strain into an amplification culture medium according to the proportion, wherein the proportion of the secondary culture solution to the amplification culture medium is 1.2: 9, then placing the mixture into a fermentation tank, introducing sterile air to control the dissolved oxygen to be 7mg/L, and fermenting for 2 hours;

5.2) anaerobic fermentation: and (3) after aerobic fermentation, the introduction of sterile air is canceled, and sealed fermentation is carried out for 35.5 hours to obtain the sludge reduction microbial agent.

The expanding culture medium in the step 5) comprises 1.2 parts of rice vinegar, 1.1 parts of brown sugar, 1.2 parts of amino acid peptide chelating agent and 65 parts of water by mass.

And 5) controlling the fermentation heat by using a water circulation heat and cold exchange system in the fermentation process to keep the fermentation temperature at 36 ℃.

Example 3

The sludge reduction microbial agent comprises the following components in parts by mass: 30 parts of lactic acid bacteria, 20 parts of saccharomycetes, 5 parts of acetic acid bacteria, 10 parts of photosynthetic bacteria, 10 parts of actinomycetes, 10 parts of archaea and 15 parts of bacillus.

1) and strain activation: respectively inoculating nonpathogenic lactobacillus, microzyme, acetic acid bacteria, photosynthetic bacteria, actinomycetes, archaebacteria and bacillus into respective test tube slant culture medium, culturing for 2-5 days in an incubator at 35 ℃, and preserving in a refrigerated cabinet at 6 ℃ for later use after the culture is finished;

2) and preparing a triangular flask culture medium: preparing culture medium corresponding to each strain according to a certain proportion, placing the culture medium into a triangular flask, sterilizing by using a culture medium sterilization cabinet at 120 ℃ and 105KP, and placing the culture medium into a sterile room to cool to normal temperature after sterilization;

3) and first-stage culture: respectively inoculating the strains obtained in the step 1) into the corresponding triangular flask culture medium obtained in the step 2) in an aseptic environment, and performing shaking culture for 48 hours at the culture temperature of 35 ℃ to obtain primary culture solutions of the strains;

4) and secondary culture: taking the primary culture solution of each strain as a seed solution, inoculating into respective triangular flask culture medium again, and performing shake culture for 48 hours at the culture temperature of 35 ℃ to obtain a secondary culture solution of each strain;

5) and (3) expanding culture:

5.1), aerobic fermentation: inoculating the secondary culture solution of each strain into an amplification culture medium according to the proportion, wherein the proportion of the secondary culture solution to the amplification culture medium is 1: 9, then placing the mixture into a fermentation tank, introducing sterile air to control the dissolved oxygen to be 7mg/L, and fermenting for 1 hour;

5.2) anaerobic fermentation: and (3) after aerobic fermentation, the introduction of sterile air is canceled, and sealed fermentation is carried out for 34.5 hours to obtain the sludge reduction microbial agent.

The expanding culture medium in the step 5) comprises 1 part of rice vinegar, 1 part of brown sugar, 1 part of amino acid peptide chelating agent and 60 parts of water by mass.

And 5) controlling the fermentation heat by using a water circulation heat and cold exchange system in the fermentation process to keep the fermentation temperature at 38 ℃.

The use method of the sludge reduction microbial agent comprises the following steps:

s1, determining the adding amount:

determining the adding proportion according to the indexes of daily treated water quantity of a system, COD load of raw water and the like and the process design characteristics of a combination device, wherein the urban sewage is generally controlled to be 0.005-0.02%, the chemical sewage is generally controlled to be 0.01-0.04%, and the adding quantity (the water fluctuation is less than or equal to 10%) is determined according to the adding proportion and the daily treated water quantity;

s2, adding:

properly increasing the sludge concentration in the system, adding the sludge reduction microbial agent which is 6-8 times of the adding amount determined in the step S1 from the water inlet or the sludge return port of the aerobic tank, and supplementing and adding the sludge reduction microbial agent according to the adding amount determined in the step S1 every month. Under the conditions of not influencing the biochemical effect of the system and not influencing the quality of biochemical effluent water of the system, the sludge concentration of the system is gradually improved by reducing the discharge amount of excess sludge.

The supplementary feeding in the step S2 is divided into 1-2 times.

Application example winnowing petrochemical water purification plant

The using date: 2011 to the present

Use of: pure oxygen two system (II) comparison: pure oxygen system (I)

Sludge reduction rate: the sludge reduction rate of the two systems is 56.3 percent compared with that of the one system.

And (3) effluent quality: the quality of the effluent water produced by using the product is not affected.

MLSS represents mixed liquor suspended solids concentration; SV30 represents the volume percentage of the sludge after the mixed liquid in the aeration tank is statically settled for 30min in the measuring cylinder, and SVI represents the sludge volume index.

The clean sewage treatment device is responsible for treating the production sewage discharged by each production device of the Yangzi petrochemical company, the domestic sewage discharged by the production area and the living area and the production sewage discharged by Yangzi-Basff company (called Yangzi company for short), and the treatment capacity is designed to be 2850m³/h。

The pure oxygen series is mainly used for treating oil refining wastewater and ethylene wastewater.

The sludge reduction is synchronous comparison, a II series which is relatively unstable in operation is selected from the two pure oxygen series for sludge reduction, and through the adding use of the sludge reduction microbial agent, after 3 months of reduction summary, on the basis that the water quality of inlet and outlet water is relatively stable, each water outlet data of a reduction group is obviously improved compared with that before reduction, and compared with a comparison group, the method has no negative influence on the water quality and plays a certain role in promoting the degradation effect of ammonia nitrogen. Meanwhile, after a decrement period of three months, the sludge decrement rate reaches 56.3 percent through process optimization control.

Claims

1. The sludge reduction microbial agent is characterized by comprising the following components in parts by mass: 26-34 parts of lactic acid bacteria, 17-23 parts of yeast, 3-6 parts of acetic acid bacteria, 8-13 parts of photosynthetic bacteria, 7-12 parts of actinomycetes, 8-12 parts of archaea and 12-18 parts of bacillus.

2. The method for preparing a sludge-reducing microbial inoculant according to claim 1, wherein the method comprises the following steps:

5) and (3) expanding culture:

3. The method for preparing a microbial agent for sludge reduction according to claim 2, wherein the expanding medium in the step 5) comprises, by mass, 0.8 to 1.2 parts of rice vinegar, 0.9 to 1.1 parts of brown sugar, 0.8 to 1.2 parts of 40% edible alcohol, and 55 to 65 parts of water.

4. The method for preparing a microorganism bacterium agent for sludge reduction according to claim 1, wherein the fermentation temperature in the step 5) is maintained at 37 ± 1 ℃ by controlling the fermentation heat with a water circulation heat and cold exchange system.

5. The method for using the microorganism bacterium agent for sludge reduction according to claim 1, comprising the steps of:

s1, normal monthly dosage:

according to the daily treated water quantity of the system, indexes such as the water quality of raw water, system design parameters, sludge load and the like, and process design characteristics of a combined device, combined with design and use experience, the urban sewage is generally controlled to be 0.005-0.01 percent, the chemical sewage is generally controlled to be 0.01-0.04 percent, and the adding amount (the water fluctuation is less than or equal to 10 percent) is determined according to the adding proportion and the daily treated water quantity; for example, after a municipal sewage treatment plant with 10 ten thousand of water treated per day operates normally, the normal dosage per month is 5-10 tons;

s2, adding:

6. The method for using the sludge reduction microbial agent as claimed in claim 1, wherein the supplementary feeding in the step S2 is performed 1-2 times.