CN111321129B - 工程化酮还原酶多肽及其应用 - Google Patents

工程化酮还原酶多肽及其应用 Download PDF

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CN111321129B
CN111321129B CN201811537509.4A CN201811537509A CN111321129B CN 111321129 B CN111321129 B CN 111321129B CN 201811537509 A CN201811537509 A CN 201811537509A CN 111321129 B CN111321129 B CN 111321129B
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陈海滨
尚传洋
蔡宝琴
马可博科拉
石淑敏
洪瑞梅
孙磊
黄勇开
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Enzymaster Ningbo Bio Engineering Co Ltd
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Abstract

本公开内容提供了可用于在工业相关条件下不对称合成手性醇化合物的工程化酮还原酶多肽的氨基酸序列、制备方法及其反应工艺。本公开内容还提供了编码工程化酮还原酶多肽的多核苷酸序列、能够表达工程化酮还原酶多肽的重组工程菌和使用工程化酮还原酶多肽生产手性醇化合物的方法。与其他制备方法相比，本发明提供的工程化酮还原酶多肽的活性和热稳定性更好，允许使用热处理的方式对酶液进行提纯，非常有利于酶的生产及酶促反应的工业化应用，大大简化了手性醇化合物的生产过程，降低了生产的成本及对环境的影响，具有很好的工业应用前景。

Description

工程化酮还原酶多肽及其应用
技术领域
本发明属于生物工程技术领域，具体涉及一种工程化酮还原酶多肽及其应用。
背景技术
手性醇类化合物是一类应用非常广泛的化合物，特别是很多天然的、具有生物活性的化合物大都含有手性醇的结构。在工业生产中，化学法和酶催化法是制备手性醇的主要方法。在酶催化法中，通常以羰基化合物为底物，通过酮还原酶(keto reductase，简称KRED)或醇脱氢酶(Alcohol dehydrogenase，简称ADH)将羰基底物不对称还原得到手性醇。酶法以其高选择性、高转化率、反应条件温和、成本较低及污染小等优势往往受到业界的青睐。在反应机理上，酮还原酶或醇脱氢酶通过辅因子NADH或NADPH提供的还原力H将羰基(或酮基)化合物催化还原成醇类化合物，同时辅因子NADH或NADPH被分别转化为NAD+或NADP+(图1)。在这个过程进行的同时，也可以通过另外一个酶促反应将NAD+或NADP+分别转化为NADH或NADPH，即完成对NADH或NADPH的循环再生。通常有三种方式来实现NAD+或NADP+分别转化为NADH或NADPH。第一种是在图1反应中同时添加异丙醇，在同一个酮还原酶或醇脱氢酶的作用下将另外一个醇类化合物(比如异丙醇)转化为羰基化合物(比如丙酮)的过程中，将NAD+或NADP+分别还原为NADH或NADPH；第二种是在图1反应中同时添加葡萄糖脱氢酶(glucose dehydrogenase)和葡萄糖，在葡萄糖脱氢酶催化葡萄糖转化为葡萄糖酸的过程中，将NAD+或NADP+分别转化为NADH或NADPH；第三种是在图1反应中同时添加甲酸脱氢酶(formate dehydrogenase)和甲酸，在甲酸脱氢酶催化甲酸转化为二氧化碳的过程中，将NAD+或NADP+分别转化为NADH或NADPH。
(R)-(-)-1,3-丁二醇(英文名为(R)-(-)-1,3-butanediol)，是一种较为昂贵的手性醇化合物，其合成较为困难。目前，国内市场上需求的高纯度的(R)-(-)-1,3-丁二醇依赖于进口。当前(R)-(-)-1,3-丁二醇的工业合成以化学法为主，以日本Daicel化工公司所报道的方法为代表，具体过程是将3-羟基丁醛通过Raney Ni催化剂进行加氢，得到消旋的1,3-丁二醇，然后进行拆分，分别得到(R)-(-)-1,3-丁二醇和(S)-(-)-1,3-丁二醇。该方法涉及到繁琐的拆分及重结晶步骤，会产生大量的废水和废渣，成本高，污染大，并且这种方法生产(R)-(-)-1,3-丁二醇的理论产率最高为50％。从酶催化的角度来看，如果能得到合适的酮还原酶，不对称地将4-羟基-2-丁酮转化为(R)-(-)-1,3-丁二醇，将到达100％的最高理论产率。但目前所报道的酮还原酶或醇脱氢酶，在应用到不对称还原4-羟基-2-丁酮来制备(R)-(-)-1,3-丁二醇时，表现出活性不高，或稳定性不足，很难直接应用于工业生产。
发明内容
1.概述
本发明提供了一种立体选择性高、催化活性高、稳定性好的工程化酮还原酶多肽，能够用于制备手性醇类化合物，特别是不对称地合成(R)-(-)-1,3-丁二醇。还提供了工程化酮还原酶多肽的基因，含有该基因的重组表达载体，工程菌株及其高效制备方法，以及使用工程化酮还原酶多肽制备手性醇类化合物的反应工艺。
发明人经过实验验证，发现文献所报道的一个来源于金黄杆菌CA49(Chryseobacterium sp.CA49)的野生型(wild type)酮还原酶ChKRED20(Journal ofMolecular Catalysis B:Enzymatic,102(2014)1–8)，对4-羟基-2-丁酮有活性，在辅因子NADH的存在下，ChKRED20可以将4-羟基-2-丁酮不对称地还原为(R)-(-)-1,3-丁二醇，ChKRED20的氨基酸序列如SEQ ID NO：2所示。但ChKRED20的活性和稳定性均不满足工业生产中反应工艺条件的要求,其性能有待提升(Applied Microbiology and Biotechnology,100(2016)3567–3575)。在工业生产中，酶的生产一般通过微生物的液体发酵来完成，在发酵过程中，宿主微生物除了生产目标酶以外，同时会生产大量的非目标酶的蛋白质(即杂蛋白)；为了去除所得酶液中的杂蛋白以达到对目标酶的一定程度的提纯，往往希望对酶液进行热处理，热处理会将酶液中的杂蛋白在热的作用下失活、沉淀并从酶液中去。热处理是一个对酶液进行纯化的非常简单、有效、经济的方法，使用经过热处理方法提纯后的酶液进行催化反应，不仅避免了杂蛋白对反应过程的影响，由于投入反应中的总蛋白量的大幅度减少，也会大大简化酶催化反应后所得产品的分离纯化过程。如果采用大肠杆菌作为宿主微生物来生产酶，经发明人验证，采用85℃对酶液进行热处理可以在工业生产条件下非常有效地将杂蛋白沉淀出来。但采用85℃对酶液热处理的前提，是目标酶要具有非常好的热稳定性，目标酶的热稳定性越好，在采用85℃热处理过程中保持不失活、不沉淀的程度就越高，损失得就越少。为了得到一个热稳定性好、活性高的酮还原酶来应用于手性醇类化合物的工业合成，本发明以不对称还原4-羟基-2-丁酮制备(R)-(-)-1,3-丁二醇的应用为参考，使用酶的定向进化技术，对ChKRED20进行了工程化改造，开发出了一系列高稳定性、高活性、高立体选择性、更广底物范围的工程化酮还原酶多肽。定向进化技术的介绍可参考“Directed Evolution:Bringing New Chemistry to Life”Frances H.Arnold,Angewandte Chemie,November 28,2017.由于对酶定向进化技术的开创性贡献，FrancesH.Arnold获得了2018年的诺贝尔化学奖。
本发明提供的改进的工程化酮还原酶多肽与SEQ ID NO：2对应的野生型的酮还原酶相比，具有更好的活性和/或稳定性，能够以极高的立体选择性不对称地制备手性醇类化合物，特别是更高效地催化4-羟基-2-丁酮转化为(R)-(-)-1,3-丁二醇，并能够同时催化异丙醇转化为丙酮，来实现NADH的循环再生(图2)。这些改进的酮还原酶多肽可包括在对应以下的残基位置与SEQ ID NO：2序列相比的一个或多个残基差异的氨基酸序列：X10，X16，X18，X20，X22，X30，X35，X38，X39，X42，X46，X52，X53，X54，X60，X64，X65，X73，X78，X80，X86，X93，X94，X97，X104，X114，X125，X126，X134，X140，X141，X142，X150，X152，X153，X164，X169，X173，X184，X185，X187，X190，X201，X204，X206，X211，X216，X218，X224，X228，X235，X239，X244。改进的酮还原酶多肽包括包含以下特征至少之一的氨基酸序列：L10V，S16R，S16K，S16N，I18V，L20Q，V22S，G30A，V35A，I38C，I38W，I38V，I38R，N39S，H42P，H42K，H42R，H42T，H42S，A46V，A46T，A52S，A52H，A52G，Q53T，G54N，V60S，V60M，T64I，S65A，S65T，L73M，V78R，I80N，I80K，I86V，I93V，G94R，G94L，G94V，G94Q，G94K，G94A，Q97K，Q97R，G104K，G104R，G104P，G104T，G104H，I114V，I114S，Y125F，E126S，E126T，E126A，E126V，G134K，G134H，G134T，G134R，N140V，M141I，M141N，M141T，A142S，A150Y，A150R，A150K，A150Q，A150N，L152Y，S153R，S153N，S153M，S153L，S153Q，V164I，V164L，N169H，E173D，V184T，G185C，G185A，A187G，E190D，M201A，M201E，A204D，A204N，I206A，M211S，M211N，K216R，E218S，V224S，V224T，S228A，M235V，M235I，Y239A，Y239S，Y239D，Y239T，Y239P，G244A，G244T，G244P；或同时在这些差异的基础上，包含1、2、3、4、5、6、7、8、9、10、11、12、13、14、15、16、18、20、21、22、23、24、25或更多个氨基酸残基的插入或缺失。
更具体的，在一些实施方案中，在SEQ ID NO：2基础上改进的工程化酮还原酶多肽包括对应SEQ ID No：4、6、8、10、12、14、16、18、20、22、24、26、28、30、32、34、36、38、40、42、44、46、48、50、52、54、56、58、60、62、64、66、68、70、72、74、76、78、80、82、84、86、88、90、92、94、96、98、100、102、104、106、108、110、112、114、116、118、120、122、124、126、128、130、132、134、136、138、140、142、144、146、148、150、152、154、156、158、160、162、164、166、168、170、172、174、176、178、180、182、184、186、188、190、192、194、196、198、200、202、204、206、208、210、212、214、216、218、220、222、224、226、228、230、232、234、236、238、240、242、244、246、248、250、252、254、256、258、260、262、264、266、268、270、272、274、276、278、280、282、284、286、288、290、292、294、296、298、300、302、304、306、308、310、312、314、316、318、320、322、324、326、328、330、332所示氨基酸序列组成的多肽。
在一些实施方案中，改进的工程化酮还原酶多肽包括与SEQ ID No：2、4、6、8、10、12、14、16、18、20、22、24、26、28、30、32、34、36、38、40、42、44、46、48、50、52、54、56、58、60、62、64、66、68、70、72、74、76、78、80、82、84、86、88、90、92、94、96、98、100、102、104、106、108、110、112、114、116、118、120、122、124、126、128、130、132、134、136、138、140、142、144、146、148、150、152、154、156、158、160、162、164、166、168、170、172、174、176、178、180、182、184、186、188、190、192、194、196、198、200、202、204、206、208、210、212、214、216、218、220、222、224、226、228、230、232、234、236、238、240、242、244、246、248、250、252、254、256、258、260、262、264、266、268、270、272、274、276、278、280、282、284、286、288、290、292、294、296、298、300、302、304、306、308、310、312、314、316、318、320、322、324、326、328、330、332的参考序列至少80％、81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％或更多序列同一性的氨基酸序列。
两条氨基酸序列或两条核苷酸序列之间的同一性均可通过本领域常用的算法得到，可采用NCBI Blastp和Blastn软件根据默认参数计算得到，也可采用采用Clustal W算法(Nucleic Acid Research,22(22):4673-4680,1994)。比如用采用Clustal W算法，SEQID NO：2与SEQ ID NO：330的氨基酸序列同一性为95.6％。
在另一方面，本发明提供了编码工程化酮还原酶多肽的多核苷酸序列。在一些实施方案中，多核苷酸可以是具有用于表达工程化酮还原酶多肽的一种或多种控制序列的表达载体的部分。在一些实施方案中，多核苷酸可包括对应SEQ ID No：3、5、7、9、11、13、15、17、19、21、23、25、27、29、31、33、35、37、39、41、43、45、47、49、51、53、55、57、59、61、63、65、67、69、71、73、75、77、79、81、83、85、87、89、91、93、95、97、99、101、103、105、107、109、111、113、115、117、119、121、123、125、127、129、131、133、135、137、139、141、143、145、147、149、151、153、155、157、159、161、163、165、167、169、171、173、175、177、179、181、183、185、187、189、191、193、195、197、199、201、203、205、207、209、211、213、215、217、219、221、223、225、227、229、231、233、235、237、239、241、243、245、247、249、251、253、255、257、259、261、263、265、267、269、271、273、275、277、279、281、283、285、287、289、291、293、295、297、299、301、303、305、307、309、311、313、315、317、319、321、323、325、327、329、331所示序列的多核苷酸序列。
如本领域技术人员所知，由于核苷酸密码子的简并性，编码SEQ ID No：4、6、8、10、12、14、16、18、20、22、24、26、28、30、32、34、36、38、40、42、44、46、48、50、52、54、56、58、60、62、64、66、68、70、72、74、76、78、80、82、84、86、88、90、92、94、96、98、100、102、104、106、108、110、112、114、116、118、120、122、124、126、128、130、132、134、136、138、140、142、144、146、148、150、152、154、156、158、160、162、164、166、168、170、172、174、176、178、180、182、184、186、188、190、192、194、196、198、200、202、204、206、208、210、212、214、216、218、220、222、224、226、228、230、232、234、236、238、240、242、244、246、248、250、252、254、256、258、260、262、264、266、268、270、272、274、276、278、280、282、284、286、288、290、292、294、296、298、300、302、304、306、308、310、312、314、316、318、320、322、324、326、328、330、332的氨基酸序列的多核苷酸序列不仅仅局限于SEQ ID No：3、5、7、9、11、13、15、17、19、21、23、25、27、29、31、33、35、37、39、41、43、45、47、49、51、53、55、57、59、61、63、65、67、69、71、73、75、77、79、81、83、85、87、89、91、93、95、97、99、101、103、105、107、109、111、113、115、117、119、121、123、125、127、129、131、133、135、137、139、141、143、145、147、149、151、153、155、157、159、161、163、165、167、169、171、173、175、177、179、181、183、185、187、189、191、193、195、197、199、201、203、205、207、209、211、213、215、217、219、221、223、225、227、229、231、233、235、237、239、241、243、245、247、249、251、253、255、257、259、261、263、265、267、269、271、273、275、277、279、281、283、285、287、289、291、293、295、297、299、301、303、305、307、309、311、313、315、317、319、321、323、325、327、329、331。本发明的酮还原酶基因的核酸序列也可以是编码序列表中SEQ ID No：4、6、8、10、12、14、16、18、20、22、24、26、28、30、32、34、36、38、40、42、44、46、48、50、52、54、56、58、60、62、64、66、68、70、72、74、76、78、80、82、84、86、88、90、92、94、96、98、100、102、104、106、108、110、112、114、116、118、120、122、124、126、128、130、132、134、136、138、140、142、144、146、148、150、152、154、156、158、160、162、164、166、168、170、172、174、176、178、180、182、184、186、188、190、192、194、196、198、200、202、204、206、208、210、212、214、216、218、220、222、224、226、228、230、232、234、236、238、240、242、244、246、248、250、252、254、256、258、260、262、264、266、268、270、272、274、276、278、280、282、284、286、288、290、292、294、296、298、300、302、304、306、308、310、312、314、316、318、320、322、324、326、328、330、332所示氨基酸序列的其他任何核酸序列。
在另一方面，本公开内容提供包含编码工程化酮还原酶的多核苷酸或能够表达工程化酮还原酶的表达载体和宿主细胞。在一些实施方案中，宿主细胞可以是细菌宿主细胞，比如大肠杆菌。宿主细胞可用于表达和分离本文所述的工程化酮还原酶，或可选地直接用于反应转化底物为产物。
在一些实施方案中，以完整细胞、粗提取物、分离的多肽或纯化的多肽形式的工程化酮还原酶可单独使用，或以固定化形式(比如固定在树脂上)使用。
本公开内容还提供了使用本文公开的工程化酮还原酶多肽将结构式(II)所示的羰基底物转化为结构式(I)所示的手性醇化合物的方法：
Figure BDA0001907261180000071
所述结构式(I)的醇产物在标为*的手性中心具有所示的立体化学构型；所示结构式(I)的醇产物与相应的对映异构体相比过量，其中
R1是任选地取代的芳基或杂芳基，或是任选地取代的C1-C8烃基，也可以是环烃基或杂环基；
R2是任选地取代的C1-C6烃基、卤素(如-F、-Cl、-Br和-I)、烯基、炔基、芳基、杂芳基、-NO2、-NO、-SO2R’或-SOR’、-SR’、-NR’R’、-OR’、-CO2R’或-COR’、-C(O)NR’、-SO2NH2或-SONH2、-CN、-CF3；其中每个R’独立地选自-H、(C1-C4)烃基、卤素、C1-C8烃基、C2-C12烯基、C2-C12炔基、环烃基、芳基或杂环基；
所述方法包括在适于转化羰基底物为醇产物的反应条件下，将结构式(II)的羰基底物和酮还原酶多肽接触，其中所述酮还原酶多肽是本文所述的工程化酮还原酶多肽。在一些实施方案中，所述工程化酮还原酶多肽与SEQ ID NO:2-332的偶数序列标识符的参考序列具有至少80％、81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％或更高的序列同一性并能够以与SEQ ID NO：2相比更优的性能(包括更高的活性，耐受更高的温度，耐受更高的有机溶剂浓度)将结构式(II)的羰基底物转化为结构式(I)的醇产物。
在一些实施方案中，结构式(I)的醇产物以至少90％、91％、92％、93％、94％、95％、96％、97％、98％或99％或更大的对映体过量产生。
在该方法的一些实施方案中，结构式(I)的醇产物是：
Figure BDA0001907261180000081
其中R3是任选地取代的C1-C4烃基、-H、卤素(如-F、-Cl、-Br和-I)、芳基、杂芳基、-NO2、-NO、-SO2R’或-SOR’、-SR’、-NR’R’、-OR’、-CO2R’或-COR’、-C(O)NR’、-SO2NH2或-SONH2、-CN、CF3，其中每个R’独立地选自-H、(C1-C4)烃基、环烃基、芳基或杂环基；
R2如上定义，且结构式(II)的羰基底物是：
Figure BDA0001907261180000082
在一些实施方案中，R3在苯基环的对位。在一些实施方案中，R3在苯基环的间位。在一些实施方案中，R3在苯基环的邻位。在一些实施方案中，R3同时在苯基环的对位和间位。在一些实施方案中，R3同时在苯基环的对位和邻位。在一些实施方案中，R3同时在苯基环的间位和邻位。
在该方法的一些实施方案中，结构式(I)的醇产物是：
Figure BDA0001907261180000083
其中R4是如以上定义的R3，R3和R2如上定义，且结构式(II)的羰基底物是：
Figure BDA0001907261180000091
在一些实施方案中，R3在苯基环的间位。在一些实施方案中，R3在苯基环的邻位。
在一些实施方案中，改进的工程化酮还原酶多肽可以用在制备对映体过量的式A2化合物(R)-(-)-1,3-丁二醇的方法中：
Figure BDA0001907261180000092
在这些实施方案中，所述方法包括在适于转化式A1化合物为式A2化合物的反应条件下，在辅因子存在下，将结构式A1化合物4-羟基-2-丁酮：
Figure BDA0001907261180000093
与本文公开的改进的工程化酮还原酶多肽接触的步骤。
在以上方法的一些实施方案中，式(I)化合物或式A2化合物以至少90％、91％、92％、93％、94％、95％、96％、97％、98％或99％或更大的对映体过量产生。
用于该方法的工程化的酮还原酶多肽的具体实施方案在详述中进一步提供。可用在以上方法中的改进的工程化酮还原酶多肽可包括选自对应SEQ ID NO：4、6、8、10、12、14、16、18、20、22、24、26、28、30、32、34、36、38、40、42、44、46、48、50、52、54、56、58、60、62、64、66、68、70、72、74、76、78、80、82、84、86、88、90、92、94、96、98、100、102、104、106、108、110、112、114、116、118、120、122、124、126、128、130、132、134、136、138、140、142、144、146、148、150、152、154、156、158、160、162、164、166、168、170、172、174、176、178、180、182、184、186、188、190、192、194、196、198、200、202、204、206、208、210、212、214、216、218、220、222、224、226、228、230、232、234、236、238、240、242、244、246、248、250、252、254、256、258、260、262、264、266、268、270、272、274、276、278、280、282、284、286、288、290、292、294、296、298、300、302、304、306、308、310、312、314、316、318、320、322的氨基酸序列。
如本文公开的使用工程化多肽用于制备式(I)化合物或式A2化合物的方法中的任一种可在一系列合适的反应条件下被执行，所述一系列合适的反应条件包括但不限于pH、温度、缓冲液、溶剂系统、底物载量、多肽载量、辅因子载量、辅因子的循环再生过程、压力和反应时间的范围。例如，在一些实施方案中，制备式(I)化合物或式A2化合物可被执行，其中合适的反应条件包括：(a)约1g/L至500g/L化合物(II)或A1的底物载量；(b)约0.1g/L至50g/L工程化多肽的载量；(c)约5g/L至500g/L异丙醇的载量；(d)约0.01g/L至1.0g/L的辅因子浓度；(e)约0％(v/v)至约99％(v/v)的有机溶剂，这里所述的有机溶剂包括但不限于4-羟基-2-丁酮、异丙醇、二甲基亚砜(DMSO)、二甲基甲酰胺(DMF)、甲基叔丁基醚(MTBE)、乙酸异丙酯、甲醇、乙醇或丙醇；(f)约4.0至约11.0的pH；和(g)约10℃至60℃的温度。
2详述
2.1定义
关于本公开内容，除非另外明确定义，否则本文说明书中使用的技术术语和科学术语具有本领域普通技术人员通常理解的含义。
“蛋白”、“多肽”和“肽”在本文可互换使用，表示由酰胺键共价连接的至少两个氨基酸的聚合物，而不论长度或翻译后修饰(如，糖基化、磷酸化、脂质化、豆蔻酰化、泛素化等等)。该定义包括D-氨基酸和L-氨基酸、以及D-氨基酸和L-氨基酸的混合物。
“工程化酮还原酶”、“工程化酮还原酶多肽”、“改进的酮还原酶多肽”和“工程化多肽”在本文可互换使用。
酮还原酶或醇脱氢酶在本文可以互换使用。
“多核苷酸”和“核酸”在本文可互换使用。
如本文使用的“辅因子”指在催化反应中与酶联合起作用的非蛋白化合物。如本文使用的，“辅因子”包括NADH(nicotinamide adenine dinucleotide)或NADPH(nicotinamide adenine dinucleotide phosphate)以及其氧化形态NAD+或NADP+，其有时也被称作辅酶。
“编码序列”指编码蛋白的氨基酸序列的核酸部分(例如，基因)。
“天然存在的”或“野生型”是指在自然界发现的形式。例如，天然存在的或野生型的多肽或多核苷酸序列是存在于生物体中、可分离自自然界中的来源且未通过人工操作有意地修饰的序列。
“重组的”或“工程化的”或“非天然存在的”当用于指例如细胞、核酸或多肽时，是指如下材料或与该材料的天然形式或固有形式相对应的材料：所述材料以自然界中不会存在的方式被改变，或与其相同但是从合成材料和/或通过使用重组技术操作而产生或获取。
“序列同一性”和“同源性”在本文可互换地用于指多核苷酸之间或多肽之间的对比(“序列同一性”和“同源性”通常以百分比的形式来表示)，且通过在比较窗口上比较两个最佳对齐的序列来确定，其中多核苷酸或多肽序列在比较窗口中的部分与参照序列相比可包括添加或缺失(即，空位)，用于两个序列的最佳对齐。百分比可以通过如下计算：确定两个序列中出现相同的核酸碱基或氨基酸残基的位置数目以产生匹配位置的数目，将匹配位置的数目除以比较窗口中位置总数并将结果乘以100以得到序列同一性百分比。可选地，该百分比可通过以下计算：确定相同核酸碱基或氨基酸残基在两个序列中都存在的位置数或核酸碱基或氨基酸残基与空位对齐的位置数以得到匹配位置数，将该匹配位置数除以比较窗口中的位置总数，并将结果乘以100以得到序列同一性的百分比。本领域技术人员将认识到，存在许多可用于比对两个序列的建立的算法。用于比较的序列最佳比对可例如通过Smith和Waterman，1981，Adv.Appl.Math.2:482的局部同源性算法、通过Needleman和Wunsch，1970，J.Mol.Biol.48:443的同源性比对算法，通过Pearson和Lipman,1988,Proc.Natl.Acad.Sci.USA85:2444的相似性搜索方法，通过这些算法的计算机实现(GCGWisconsin软件包中的GAP、BESTFIT、FASTA或TFASTA)或通过直观检查(一般参见，Current Protocols in Molecular Biology,F.M.Ausubel等编著，Current Protocols,Greene Publishing Associates Inc.和John Wiley&Sons,Inc.之间的合资企业,(1995年增刊)(Ausubel))。适宜于确定序列同一性和序列相似性百分比的算法的实例是BLAST和BLAST2.0算法，它们分别描述于Altschul等人，1990，J.Mol.Biol.215:403-410和Altschul等,1977,Nucleic Acids Res.3389-3402中。用于执行BLAST分析的软件是通过美国国家生物技术信息中心(National Center for Biotechnology Information)网站公开可用的。该算法包括首先通过鉴定查询序列中长度W的短字来鉴定高评分序列对(HSP)，所述短字与数据库序列中相同长度的字比对时匹配或满足一些正值的阀值得分T。T被称作邻近字评分阈值(Altschul等，如上述)。这些初始相邻字匹配(word hit)充当用于启始搜索的种子来寻找包含它们的更长的HSP。然后字匹配沿着每个序列在两个方向延伸到累积比对得分不能够增加的程度。对于核苷酸序列，累积得分使用参数M(对于匹配残基对的奖励得分；永远＞0)和N(对于错配残基的惩罚得分；永远＜0)计算。对于氨基酸序列，得分矩阵被用于计算累计得分。当以下情况时，每个方向中的字匹配字串的延伸被终止：累积比对得分从其最大达到值下降了量X；由于累积一个或多个负得分残基比对，累积得分达到0或以下；或到达任一序列末端。BLAST算法参数W、T和X决定比对的灵敏度和速度。BLASTN程序(用于核苷酸序列)使用字长(W)11、期望值(E)10、M＝5、N＝-4以及两链的比较作为默认值。对于氨基酸序列，BLASTP程序使用以下作为缺省值：字长(W)为3，期望值(E)为10和BLOSUM62得分矩阵(见Henikoff和Henikoff,1989,Proc Natl Acad Sci USA89：10915)。序列比对和序列同一性％的示例性确定可使用GCG Wisconsin软件包(Accelrys,Madison WI)中的BESTFIT或GAP程序，使用所提供的缺省参数。
“参考序列”是指用作序列比较的基础的限定序列。参考序列可以是较大序列的子集，例如，全长基因或多肽序列的片段。一般而言，参考序列为至少20个核苷酸或氨基酸残基长，至少25个残基长，至少50个残基长，或者核酸或多肽的全长。因为两种多核苷酸或多肽可以各自(1)包括在两种序列之间相似的序列(即，完整序列的一部分)，且(2)可以进一步包括在两种序列之间不同的序列，两种(或更多)多核苷酸或多肽之间的序列比较通常通过在“比较窗口”内比较两种多核苷酸或多肽的序列来进行以鉴定和比较序列相似性的局部区域。在一些实施方案中，“参照序列”不意为限于野生型序列，且可包括工程化或改变的序列。例如，“在对应于X94的残基处具有赖氨酸的基于SEQ ID NO:2的参考序列”指其中在SEQ ID NO:2中的X40处相应的残基(是甘氨酸)已经改变成赖氨酸的参考序列。
“比较窗口”指至少约20个相邻核苷酸位置或者氨基酸残基的概念性片段，其中序列可以与至少20个相邻核苷酸或氨基酸的参考序列相比较，并且其中比较窗口中的序列的部分可以包括与参考序列(其不包括添加或缺失)相比20％或更少的添加或缺失(即，空位)，用于两序列的最佳比对。比较窗口可以长于20个相邻残基，并且包括任选地30、40、50、100或更长的窗口。
在用于指定的氨基酸或多核苷酸序列的编号的情况下，"相应于"、"参考于"或"相对于"指当指定的氨基酸或多核苷酸序列与参考序列相比较时指定的参考序列残基的编号。换言之，给定序列的残基编号或残基位置是根据参考序列指定的，而不是给定氨基酸或多核苷酸序列内的残基的实际数字位置指定的。例如，可以将给定的氨基酸序列诸如工程化酮还原酶的氨基酸序列与参考序列进行比对，这是通过引入空位以优化这两条序列之间的残基匹配而实现的。在这些情况下，虽然存在空位，但是给定氨基酸或多核苷酸序列中的残基编号相对于已与其比对的参考序列制定。
“氨基酸差异”或“残基差异”指在多肽序列的一个位置处氨基酸残基相对于参考序列中相应位置处的氨基酸残基的差异。本文中氨基酸差异的位置一般被称为“Xn”，其中n指残基差异基于其的参考序列中的相应位置。例如，“与SEQ ID NO:2相比在位置X94处的残基差异”指在相应于SEQ ID NO:2的位置94的多肽位置处的氨基酸残基的差异。因此，如果SEQ ID NO:2的参考多肽在位置94处具有甘氨酸，那么“与SEQ ID NO:2相比在位置X40处的残基差异”是指在相应于SEQ ID NO:2的位置94的多肽位置处除了甘氨酸之外的任何残基的氨基酸取代。在本文的大多数实例中，在一个位置处的特定氨基酸残基差异表示为“XnY”，其中“Xn”指如以上描述的对应位置，并且“Y”为在工程化多肽中发现的氨基酸的一字母标识符(即，与参考多肽中的不同的残基)。在一些实例中(例如，在表1中)，本公开内容还提供由常规符号“AnB”表示的特定氨基酸差异，其中A为参考序列中的残基的一字母标识符，“n”为在参考序列中的残基位置的编号，并且B为工程化多肽的序列中残基取代的单字母标识符。在一些实例中，本公开内容的多肽可包含相对于参考序列的一个或更多个氨基酸残基差异，其通过相对于参考序列存在残基差异处的特定位置的列表表示。
“缺失”指通过从参考多肽去除一个或更多个氨基酸而对多肽的修饰。缺失可以包括除去1个或多个、2个或多个氨基酸，5个或多个氨基酸，10个或多个氨基酸，15个或多个氨基酸，或20个或多个氨基酸，多达组成参照酶的氨基酸总数的10％，或多达组成参照酶的氨基酸总数的20％，同时保留工程化酮还原酶的酶活性和/或保留工程化酮还原酶的改进的性质。缺失可以涉及多肽的内部部分和/或末端部分。在各种实施方案中，缺失可以包括连续的区段或者可以是不连续的。
"插入"指通过从参考多肽添加一个或多个氨基酸的多肽的修饰。在一些实施方案中，改进的工程化酮还原酶包括一个或更多个氨基酸插入天然存在的酮还原酶多肽，以及一个或更多个氨基酸插入其他改进的酮还原酶多肽。可在多肽的内部部分插入，或插入到羧基或氨基末端。如本文所用的，插入包括本领域中已知的融合蛋白。插入可以是氨基酸的连续区段，或者被在天然存在的多肽中一个或更多个氨基酸分隔。
如本文所用的"片段"指具有氨基末端和/或羧基末端缺失、但是其中保留的氨基酸序列与序列中相应的位置相同的多肽。片段可以为至少10个氨基酸长、至少20个氨基酸长，至少50个氨基酸长或更长，以及高达全长酮还原酶多肽的70％、80％、90％、95％、98％和99％。
“分离的多肽”是指如下多肽：所述多肽基本上与其天然伴随的其他物质例如蛋白、脂质和多核苷酸分离。该术语包括已从其天然存在的环境或表达系统(例如，宿主细胞或体外合成中)移去或纯化的多肽。改进的酮还原酶多肽可以存在于细胞内、存在于细胞培养基中或者以各种形式制备，诸如裂解物或分离的制备物。像这样，在一些实施方案中，改进的酮还原酶多肽可以是分离的多肽。
“手性中心”是指连接四个不同基团的碳原子。
“立体选择性”(stereoselectivity)指在化学或酶促反应中一种立体异构体相对于另一种或多种异构体的优先形成。立体选择性可以是部分的，其中一种立体异构体的形成优于另一种异构体；或可以是完全的，其中仅形成一种立体异构体。当立体异构体是对映异构体时(enantiomers)，立体选择性被称为对映异构体选择性(enantioselectivity)，一种对映异构体在两种对映异构体的混合物之中的过量分数(通常被报告为百分比)通常被可选地报告为“对映异构体过量”(enantiomeric excess，简称为ee)。当立体异构体是非对映异构体时(diastereoisomers)，立体选择性被称为非对映异构体选择性(diastereoselectivity)，即一种非对映异构体在两种非对映异构体的混合物之中的过量分数(通常被报告为百分比)，通常被可选地报告为“非对映异构体过量”(diastereoisomeric excess，简称为de)。在本领域内该分数(典型地为百分比)通常可选择地报道为根据下式从中计算的对映异构体过量(enantiomeric excess，即ee)：{主要对映异构体浓度–次要对映异构体浓度}/{主要对映异构体浓度+次要对映异构体浓度}。
“立体异构体”、“立体异构形式”和类似表述在本文可互换使用，是指分子差异仅在其原子在空间中的方位不同所造成的所有异构体。其包括对映异构体和具有多于一个手性中心、且彼此不是镜像的化合物的异构体(即“非对映异构体”)。
“改进的酶性质”指与参考酮还原酶相比显示任何酶性质的改进的酮还原酶多肽，所述参考酮还原酶诸如野生型酮还原酶或另一种改进的工程化酮还原酶。期望改进的酶性质包括，但不限于，酶活性(其可以底物的转化百分比的方式被表示)、热稳定性、溶剂稳定性、pH活性特征、辅因子需求、对抑制物的耐受性(例如，底物或产物抑制)、立体特异性和立体选择性。
“转化”指底物向相应的产物的酶转化。“转化百分比”或“转化率”是指在指定反应条件下在指定的反应时间内，反应体系中被转化为产物的底物的百分比。因此，酮还原酶多肽的“酶活性”或“活性”可以被表示为底物向产物的“转化百分比”。转化率一般通过取样来测定反应体系中的产物浓度和底物浓度来计算：{产物摩尔浓度}/{底物摩尔浓度+产物摩尔浓度}。
“热稳定的”指与野生型酶相比酮还原酶多肽在暴露于升高的温度(例如72℃或更高温度)持续一段时间(例如2.5小时或更长时间)之后维持相似活性。
“溶剂稳定的”或“溶剂耐受的”指与野生型酶相比酮还原酶多肽在暴露于不同浓度(例如5-99％)的溶剂(甲醇、乙醇、异丙醇、二甲基亚砜(DMSO)、四氢呋喃、2-甲基四氢呋喃、丙酮、甲苯、乙酸丁酯、甲基叔丁基醚等)持续一段时间(例如0.5-24小时)之后维持相似的活性。
“合适的反应条件”指生物催化反应溶液中的那些条件(例如，酶载量、底物载量、辅因子载量、温度、pH、缓冲液、共溶剂等的范围)，也包括对辅因子循环再生的反应物质，在该条件下本公开内容的酮还原酶多肽能将底物转化成期望的产物化合物。示例性“合适的反应条件”被提供于本公开内容并通过实施例例证。
“烃基”是指直链或支链的烃基团。符号“C”后出现下标的数字规定了特定基团可包含的碳原子数。例如，“C1-C8”是指具有1至8个碳原子的直链或支链烃基基团。烃基基团可任选第被一个或多个取代基基团取代。“芳基”是指6至约20个碳原子的单价芳香族烃基。“杂芳基”和“杂芳族”是指其中母体芳环系统的一个或多个碳原子被杂原子(O，N或S)代替的芳基基团。“取代的”当用于修饰指定基团或自由基时，是指指定基团或自由基的一个或多个氢原子各自彼此独立地本文所用的“化合物”是指伴随本文公开的化合物标明的结构式和/或化学名涵盖的任何化合物。化合物可以其化学结构和/或化学名标明。当化学结构与化学名冲突时，化学结构决定化合物的身份。除非另外具体地描述或指出，否则本文描述的化学结构涵盖所描述化合物的所有可能异构体形式。
被相同或不同的取代基代替。“取代的烃基、芳基或杂芳基”指其中一个或多个氢原子用另一取代基代替的烃基、芳基或杂芳基基团。“任选”或“任选地”是指所描述的事件或情况可能发生或不发生；例如“任选地取代的芳基”是指可能被取代或可能未被取代的芳基基团，这一描述包括取代的芳基基团和未取代的芳基基团二者。
2.2改进的工程化酮还原酶以下表1举例说明本发明所开发的工程化酮还原酶多肽。每一行给出一个具体的工程化酮还原酶多肽的核苷酸序列编号和氨基酸序列编号，以及与SEQ ID No:2相比较的残基差异。每个举例的工程化酮还原酶多肽的催化性能(综合了酶在反应中的整体表现，包括但不限于活性、热稳定性、在反应体系中的稳定性、对产物的立体选择性等等)以“+”表示，具体的含义见表2或表3。
表1
Figure BDA0001907261180000161
Figure BDA0001907261180000171
Figure BDA0001907261180000181
Figure BDA0001907261180000191
Figure BDA0001907261180000201
表2
Figure BDA0001907261180000211
表2所述的反应条件中的酶液，其制备过程参考实施例2，酶液没有经过任何热处理，所制得的酶液中的蛋白浓度约为10g/L(蛋白浓度的测定使用本领域熟知的Bradford方法)，且含有等量的表1中氨基酸序列对应的酮还原酶多肽。表2中的反应在40℃、中性pH下进行，反应的操作过程可参考实施例10。
表3
Figure BDA0001907261180000212
Figure BDA0001907261180000221
表3反应条件中所述的热处理后的酶液，是在表2所述的酶液基础上，进行热处理后得到的酶液。热处理的操作过程可参考实施例3。表3中的反应在40℃、中性pH下进行，反应的操作过程可参考实施例10。
由于表1中氨基酸序列对应的酮还原酶多肽的热稳定性存在差异，经热处理后，不同的酮还原酶多肽会表现出不同程度的失活而导致催化性能的变化，体现在表2和表3所示的转化率的变化上。
2.3可用于制备工程化酮还原酶多肽的多核苷酸、控制序列、表达载体和宿主细胞
在另一方面，本公开内容提供了编码本文描述的具有酮还原酶活性的工程化多肽的多核苷酸。多核苷酸可与控制基因表达的一种或多种异源调控序列可操作地连接以产生能够表达多肽的重组多核苷酸。包含编码工程化的酮还原酶的异源多核苷酸的表达构建体可被引入合适的宿主细胞以表达相应的工程化酮还原酶多肽。
如对本领域技术人员明显的是，蛋白序列的可得性和相应于多种氨基酸的密码子的知识提供能够编码目标蛋白序列的所有多核苷酸的说明。其中相同氨基酸由可选择的或同义密码子编码的遗传密码的简并性允许产生极大数目的核酸，所有这些核酸编码本文公开的改进的酮还原酶多肽。因此，确定特定的氨基酸序列后，本领域的技术人员可以以不改变蛋白质的氨基酸序列的方式通过仅仅修饰一个或多个密码子的序列来产生任何数目的不同的核酸。在这点上，本公开内容特别地构思了可通过基于可能的密码子选取而选择组合来制备的多核苷酸的各个和每个可能的改变，并且对于本文公开的任何多肽，包括在表1中提供的示例性工程化多肽的氨基酸序列，以及在通过引用并入本文的序列表中作为SEQID NO:4-332的偶数序列标识符的序列公开的任何多肽，所有这些改变被认为特别地公开。
在多种实施方案中，密码子被优选地选择以适应在其中产生蛋白的宿主细胞。例如，用于细菌的优选密码子用于表达细菌中的基因；用于酵母中的优选密码子用于在酵母中表达；且用于哺乳动物的优选密码子用于在哺乳动物细胞中表达。
在一些实施方案中，所述多核苷酸编码包含与选自SEQ ID NO:4-332的偶数序列标识符的参考序列至少约80％、81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％或更多序列同一性的氨基酸序列的酮还原酶多肽，其中所述多肽具有酮还原酶活性以及本文描述的改进的特性中的一种或更多种，例如以与SEQ ID NO:2的多肽相比增加的活性和/或稳定性将化合物A1转化成化合物A2的能力。
在一些实施方案中，所述多核苷酸编码工程化酮还原酶多肽，所述工程化酮还原酶多肽包含与SEQ ID NO:2相比具有以上描述的同一性百分比并且具有选自的一个或更多个氨基酸残基差异的氨基酸序列。在一些实施方案中，本公开内容提供了具有酮还原酶活性的工程化多肽，所述工程化多肽包含与SEQ ID NO:2的参考序列具有至少80％的序列同一性以及具有选自以下位置的残基差异的组合：X10，X16，X18，X20，X22，X30，X35，X38，X39，X42，X46，X52，X53，X54，X60，X64，X65，X73，X78，X80，X86，X93，X94，X97，X104，X114，X125，X126，X134，X140，X141，X142，X150，X152，X153，X164，X169，X173，X184，X185，X187，X190，X201，X204，X206，X211，X216，X218，X224，X228，X235，X239，X244。
在一些实施方案中，编码工程化酮还原酶多肽的多核苷酸包含选自SEQ ID NO:3-331的奇数序列标识符的序列。
在一些实施方案中，所述多核苷酸编码本文所述的多肽，但是在核苷酸水平上与编码工程化酮还原酶的参考多核苷酸具有约50％、55％、60％、65％、70％、75％、80％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％或99％或更高的序列同一性。在一些实施方案中，参考多核苷酸序列选自具有SEQ ID NO:3-331的奇数序列标识符的序列。
编码工程化酮还原酶多肽的分离的多核苷酸可以多种方法被操作以提供多肽的表达，所述方法包括通过密码子优化来进一步改变序列以改进表达、在合适的有或无另外的控制序列的表达元件中插入、和转化入适于表达并产生多肽的宿主细胞。
取决于表达载体，在分离的多核苷酸插入载体之前对分离的多核苷酸的操作可以是期望的或必需的。利用重组DNA方法修饰多核苷酸和核酸序列的技术在本领域中是公知的。在以下中提供了指导：Sambrook等人,2001,MolecuLar Cloning:A LaboratoryManual,第三版,Cold Spring Harbor Laboratory Press；和Current Protocols inMolecuLar Biology,Ausubel.F.编著,GreenePub.Associates,1998,2010年更新。
在另一个方面，本公开还涉及重组表达载体，根据它们将被导入的宿主的类型，其包括编码工程化酮还原酶多肽或其变体的多核苷酸，和一个或多个表达调节区，诸如启动子和终止子、复制起点等等。可选地，本公开内容的核酸序列可以通过使核酸序列或包括该序列的核酸构建体插入到适当的表达载体中来表达。在产生表达载体时，编码序列位于载体中以使编码序列被可操作地连接于用于表达的合适的控制序列上。
重组表达载体可为任何载体(例如，质粒或病毒)，其可方便地应用于重组DNA步骤中并且可带来多核苷酸序列的表达。载体的选择通常将取决于载体与待引入载体的宿主细胞的相容性。载体可以是线性或闭合环状的质粒。表达载体可以是自主复制的载体，即作为染色体外的实体而存在的载体，它的复制独立于染色体的复制，如质粒、染色体外的元件、微小染色体、或人工染色体。载体可以包含用于保证自我复制的任何工具。可选地，载体可以是在被引入到宿主细胞中时整合到基因组中并且与其所整合到的染色体一起复制的载体。而且，可以使用单一载体或质粒或者一起包含待引入到宿主细胞基因组中的总DNA的两种或多种载体或质粒。
对本公开内容的实施方案有用的很多表达载体是商业上可得的。示例性表达载体可通过将编码改进的酮还原酶多肽的多核苷酸可操作地连接到质粒pACYC-Duet-1(Novagen)中来制备。
在另一方面，本公开内容提供包含编码本公开内容的改进的酮还原酶多肽的多核苷酸的宿主细胞，所述多核苷酸被可操作地连接至在宿主细胞中用于酮还原酶的表达的一个或更多个控制序列。用于表达由本公开内容的表达载体编码的多肽的宿主细胞在本领域是公知的，并且包括但不局限于诸如大肠杆菌、节杆菌属种KNK168、链霉菌属和鼠伤寒沙门氏菌(Salmonella typhimurium)细胞的细菌细胞；诸如酵母细胞(例如，酿酒酵母或巴斯德毕赤氏酵母(Pichia pastoris))的真菌细胞；诸如果蝇S2以及灰翅夜蛾(Spodoptera)Sf9细胞的昆虫细胞；诸如CHO、COS、BHK、293和Bowes黑色素瘤细胞的动物细胞；以及植物细胞。示例性的宿主细胞为大肠杆菌BL21(DE3)。上述宿主细胞可以是野生型的，也可以是经过基因组编辑的工程细胞，比如把宿主细胞基因组中所携带的野生型的酮还原酶基因敲除。上述宿主细胞的合适的培养基以及生长条件在本领域内是公知的。
用于表达酮还原酶的多核苷酸可通过本领域已知的多种方法被引入至细胞。技术包括，除了其他以外，电穿孔、生物颗粒轰击法、脂质体介导的转染、氯化钙转染和原生质体融合。将多核苷酸引入细胞的不同方法对于本领域技术人员是明显的。
2.4产生工程化酮还原酶多肽的方法
通过将编码酮还原酶的多核苷酸经历诱变和/或定向进化方法，可以获得工程化酮还原酶。示例性的定向进化技术可见《Biocatalysis for the PharmaceuticalIndustry:Discovery,Development,and Manufacturing》(2009John Wiley&Sons Asia(Pte)Ltd.ISBN:978-0-470-82314-9)。
当工程化多肽的序列为已知时，编码多肽的多核苷酸可根据已知的合成方法通过标准的固相方法制备。在一些实施方案中，多达约100个碱基的片段可单独地合成，然后连接(例如，通过酶促或化学的连接方法或聚合酶介导的方法)以形成任何需要的连续序列。例如，本公开内容的多核苷酸和寡核苷酸可通过化学合成制备，使用，例如，描述于Beaucage等人,1981,TetLett22：1859-69的经典的亚磷酰胺方法，或描述于Matthes等人,1984,EMBOJ.3:801-05的方法，例如，如在自动化合成方法中典型地实践的。根据亚磷酰胺方法，寡核苷酸在例如，在自动化DNA合成仪中合成、纯化、退火、连接以及克隆至合适的载体中。另外，基本上任何核酸可从多种商业来源的任一个获得。
在一些实施方案中，本公开内容还提供了用于制备或制作工程化酮还原酶多肽的方法，其中该方法包括在适于表达多肽的培养条件下培养能够表达编码工程化多肽的多核苷酸的宿主细胞。在一些实施方案中，制备多肽的方法还包括分离多肽。工程化多肽可在合适的细胞中表达，并利用所熟知的用于蛋白纯化的技术中的任何一种或更多种从宿主细胞和/或培养基分离(或回收)，所述用于蛋白纯化的技术包括，除了其他以外，溶菌酶处理、超声、过滤、盐析、超速离心和色谱。
2.5利用工程化酮还原酶的方法以及用其制备的化合物
在另一方面，本文所述的改进的工程化酮还原酶多肽，在辅因子NADH存在下，可将羰基化合物转化为手性醇类化合物。本公开内容还提供了使用本文公开的工程化酮还原酶多肽制备广泛范围的化合物(I)或其结构类似物的方法。在一些实施方案中，工程化酮还原酶多肽可用在制备结构式(I)的化合物的方法中：
Figure BDA0001907261180000261
所述结构式(I)的醇产物在标为*的手性中心具有所示的立体化学构型；所示结构式(I)的醇产物与相应的对映异构体相比过量。其中
R1是任选地取代的芳基或杂芳基，或是任选地取代的C1-C8烃基，也可以是环烃基或杂环基；
R2是任选地取代的C1-C6烃基、卤素(如-F、-Cl、-Br和-I)、烯基、炔基、芳基、杂芳基、-NO2、-NO、-SO2R’或-SOR’、-S R’、-N R’R’、-O R’、-CO2R’或-COR’、-C(O)NR’、-SO2NH2或-SO NH2、-CN、-CF3；其中每个R’独立地选自-H、(C1-C4)烃基、卤素、C1-C8烃基、C2-C12烯基、C2-C12炔基、环烃基、芳基或杂环基。
所述方法包括在适于转化羰基底物为醇产物的反应条件下，将结构式(II)的羰基底物
Figure BDA0001907261180000271
和酮还原酶多肽接触，其中所述酮还原酶多肽是本文所述的工程化酮还原酶多肽。
在一些实施方案中，所述工程化酮还原酶多肽与SEQ ID NO:2-332的偶数序列标识符的参考序列具有至少80％、81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％或更高的序列同一性并能够以与SEQID NO：2相比更优的性能(包括更高的活性，耐受更高的温度，耐受更高的有机溶剂浓度)将结构式(II)的羰基底物转化为结构式(I)的醇产物。
在一些实施方案中，结构式(I)的手性醇类化合物以至少90％、91％、92％、93％、94％、95％、96％、97％、98％、99％或更大的对映体过量产生。
如上所述，可用于本公开内容的方法中的工程化酮还原酶多肽可按照其转化4-羟基-2-丁酮为(R)-(-)-1,3-丁二醇的能力来表征。因此，在本文公开的方法的任何实施方案中，可进行该方法，其中工程化酮还原酶多肽能够以与SEQ ID NO：2相比更优的性能将4-羟基-2-丁酮转化为(R)-(-)-1,3-丁二醇，并与SEQ ID NO:2-332的偶数序列标识符的参考序列具有至少80％、81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％或更大的氨基酸序列同一性。
在该方法的一些实施方案中，结构式(I)的醇产物是：
Figure BDA0001907261180000272
其中R3是任选地取代的C1-C4烃基、-H、卤素(如-F、-Cl、-Br和-I)、芳基、杂芳基、-NO2、-NO、-SO2R’或-SOR’、-S R’、-N R’R’、-O R’、-CO2R’或-COR’、-C(O)NR’、-SO2NH2或-SONH2、-CN、CF3，其中每个R’独立地选自-H、(C1-C4)烃基、环烃基、芳基或杂环基；
R2如上定义，且结构式(II)的羰基底物是：
Figure BDA0001907261180000281
在一些实施方案中，R3在苯基环的对位。在一些实施方案中，R3在苯基环的间位。在一些实施方案中，R3在苯基环的邻位。在一些实施方案中，R3同时在苯基环的对位和间位。在一些实施方案中，R3同时在苯基环的对位和邻位。在一些实施方案中，R3同时在苯基环的间位和邻位。
在该方法的一些实施方案中，结构式(I)的醇产物是：
Figure BDA0001907261180000282
其中R4是如以上定义的R3，R3和R2如上定义，且结构式(II)的羰基底物是：
Figure BDA0001907261180000283
在一些实施方案中，R3在苯基环的间位。在一些实施方案中，R3在苯基环的邻位。
在该方法的一些实施方案中，结构式(I)的手性醇产物是(R)-(-)-3-羟基丁酸甲酯：
Figure BDA0001907261180000284
且结构式(II)的羰基底物是乙酰乙酸甲酯：
Figure BDA0001907261180000285
在该方法的一些实施方案中，结构式(I)的手性醇产物是(R)-(-)-3-羟基丁酸乙酯：
Figure BDA0001907261180000291
且结构式(II)的羰基底物是乙酰乙酸乙酯：
Figure BDA0001907261180000292
在该方法的一些实施方案中，结构式(I)的手性醇产物是(S)-(-)-4-氯-3-羟基丁酸乙酯：
Figure BDA0001907261180000293
且结构式(II)的羰基底物是4-氯乙酰乙酸乙酯：
Figure BDA0001907261180000294
在该方法的一些实施方案中，结构式(I)的手性醇产物是(R)-1-[3,5-二(三氟甲基)苯基]乙醇：
Figure BDA0001907261180000295
且结构式(II)的羰基底物是3，5-双三氟甲基苯乙酮：
Figure BDA0001907261180000296
在该方法的一些实施方案中，结构式(I)的手性醇产物是：
Figure BDA0001907261180000301
且结构式(II)的羰基底物是：
Figure BDA0001907261180000302
在一些实施方案中，在上述方法中产生的结构式(I)的醇产物以至少90％、91％、92％、93％、94％、95％、96％、97％、98％、99％或更大的对映体过量产生。
用于以上方法的工程化酮还原酶多肽的具体实施方案在详述中进一步提供。可用在以上方法中的酮还原酶多肽可包括选自对应SEQ ID NO：2、4、6、8、10、12、14、16、18、20、22、24、26、28、30、32、34、36、38、40、42、44、46、48、50、52、54、56、58、60、62、64、66、68、70、72、74、76、78、80、82、84、86、88、90、92、94、96、98、100、102、104、106、108、110、112、114、116、118、120、122、124、126、128、130、132、134、136、138、140、142、144、146、148、150、152、154、156、158、160、162、164、166、168、170、172、174、176、178、180、182、184、186、188、190、192、194、196、198、200、202、204、206、208、210、212、214、216、218、220、222、224、226、228、230、232、234、236、238、240、242、244、246、248、250、252、254、256、258、260、262、264、266、268、270、272、274、276、278、280、282、284、286、288、290、292、294、296、298、300、302、304、306、308、310、312、314、316、318、320、322、324、326、328、330、332的氨基酸序列。
在一些实施方案中，改进的工程化酮还原酶多肽可以用在制备对映体过量的式A2化合物(R)-(-)-1,3-丁二醇的方法中：
Figure BDA0001907261180000303
在这些实施方案中，所述方法包括在适于转化式A1化合物为式A2化合物的反应条件下，在辅因子NADH存在下，将结构式A1化合物4-羟基-2-丁酮：
Figure BDA0001907261180000311
与本文公开的改进的工程化酮还原酶多肽接触的步骤。
在以上方法的一些实施方案中，式A2化合物以至少90％、91％、92％、93％、94％、95％、96％、97％、98％、99％或更大的对映体过量产生。
用于以上方法的工程化酮还原酶多肽的具体实施方案在详述中进一步提供。可用在以上方法中的酮还原酶多肽可包括选自对应SEQ ID NO：2、4、6、8、10、12、14、16、18、20、22、24、26、28、30、32、34、36、38、40、42、44、46、48、50、52、54、56、58、60、62、64、66、68、70、72、74、76、78、80、82、84、86、88、90、92、94、96、98、100、102、104、106、108、110、112、114、116、118、120、122、124、126、128、130、132、134、136、138、140、142、144、146、148、150、152、154、156、158、160、162、164、166、168、170、172、174、176、178、180、182、184、186、188、190、192、194、196、198、200、202、204、206、208、210、212、214、216、218、220、222、224、226、228、230、232、234、236、238、240、242、244、246、248、250、252、254、256、258、260、262、264、266、268、270、272、274、276、278、280、282、284、286、288、290、292、294、296、298、300、302、304、306、308、310、312、314、316、318、320、322、324、326、328、330、332的氨基酸序列。
在本文描述的方法中，工程化酮还原酶多肽在辅因子NADH的存在下，催化羰基底物转化为手性醇产物。在一些实施方案中，反应过程中辅因子NADH的再生是通过在同一个工程化酮还原酶多肽的作用下，将另外一个醇类化合物(比如异丙醇)转化为羰基化合物(比如丙酮)的过程中，将NAD+转化为NADH。在一些实施方案中，反应过程中辅因子NAD+的再生是通过葡萄糖脱氢酶(glucose dehydrogenase)催化葡萄糖转化为葡萄糖酸的过程中，将NAD+转化为NADH；该方法的一个具体示例参考实施例9。在一些实施方案中，反应过程中辅因子NAD+的再生是通过甲酸脱氢酶(formate dehydrogenase)催化甲酸转化为二氧化碳的过程中，将NAD+转化为NADH。
如本领域技术人员所知，酮还原酶催化反应是可逆的。在一些实施方案中，在辅因子NAD+的存在下，本发明所公开的工程化酮还原酶多肽也可以将式(I)或式A2的手性醇类化合物分别转化为化合物(II)或A1：
Figure BDA0001907261180000321
在该方法的一些实施方案中，底物为外消旋的醇类化合物，本发明所公开的工程化酮还原酶多肽选择性地将其中的一个对映异构体转化为羰基化合物，而保留另外一个对映异构体，达到对外消旋的醇类化合物的手性拆分。在酮还原酶将醇类化合物转化为羰基化合物的过程中，可以同时实现NAD+的循环再生，比如由同一个酮还原酶将丙酮转化为异丙醇来实现。该方法的一个具体示例参考实施例15。
如本文描述并在实施例中例证的，本公开内容构思了可在本文的方法中使用的合适的反应条件的范围，包括但不限于辅因子的循环再生方法、温度、溶剂系统、底物载量、多肽载量、缓冲液、pH、和反应时间的范围。用于执行使用本文描述的工程化酮还原酶多肽催化底物化合物转化成产物化合物的方法的另外的合适的反应条件可容易地通过常规实验优化，所述常规实验包括但不限于在各个反应成分的载量、pH、温度、溶剂条件、辅因子的循环再生方法的实验反应条件下使工程化酮还原酶多肽与底物化合物接触，并检测产物化合物，例如，利用在本文提供的实施例中描述的方法。
如以上描述的，用于本公开内容的方法的具有酮还原酶活性的工程化多肽通常包含与选自SEQ ID NO:2-332的偶数序列的任一个的参考氨基酸序列具有至少80％、81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％或更高序列同一性的氨基酸序列。
考虑到例如期望的产物化合物的量、底物浓度对酶活性的影响、反应条件下酶的稳定性、和底物到产物的转化百分比，反应混合物中的底物化合物的载量可以变化。在所述方法的一些实施方案中，合适的反应条件包括至少约0.5g/L、至少约1g/L、至少约5g/L、至少约10g/L、至少约15g/L、至少约20g/L、至少约30g/L、至少约50g/L、至少约75g/L、至少约100g/L、至少约150g/L、至少约200g/L、至少约250g/L、至少约300g/L、至少约350g/L、至少约400g/L或甚至更大的底物(II)或底物A1的载量。虽然本文提供的底物载量的值是基于化合物(II)或A1的分子量，但是还预期，也可在方法中使用相等的摩尔量的化合物(II)或A1的多种水合物和盐。
在反应的实施方案中，反应条件可包括合适的pH。如以上所述，期望的pH或期望的pH范围可通过使用酸或碱、合适的缓冲剂、或缓冲和添加酸或碱的组合来保持。反应混合物的pH可在反应过程之前和/或期间控制。在一些实施方案中，合适的反应条件包括约4至约11的溶液pH。在一些实施方案中，反应条件包括约4.0、4.5、5.0、5.5、6.0、6.5、7、7.5、8、8.5、9、9.5、10、10.5、11.0的溶液pH。
在本文的方法的实施方案中，例如考虑到在较高的温度下在反应速率上的增加、对于足够久的反应持续时间的酶的活性，合适的温度可被用于反应条件。相应地，在一些实施方案中，合适的反应条件包括约10℃至约60℃、约25℃至约50℃、约25℃至约40℃、或约25℃至约30℃的温度。在一些实施方案中，合适的反应温度包括约10℃、15℃、20℃、25℃、30℃、35℃、40℃、45℃、50℃、55℃或60℃的温度。在一些实施方案中，酶促反应期间的温度可以贯穿反应过程保持在一定温度。在一些实施方案中，酶促反应期间的温度可以在反应过程期间调整为温度曲线。
使用工程化酮还原酶的方法通常在水或溶剂中进行。合适的溶剂包括水性缓冲溶液、有机溶剂和/或共溶剂系统，共溶剂系统通常包括水性溶剂和有机溶剂。水性溶液(水或水性共溶剂系统)可为pH-缓冲的或非缓冲的。在一些实施方案中，使用工程化酮还原酶多肽的方法通常地于包含以下的水性共溶剂系统中进行：有机溶剂(例如，甲醇、乙醇、丙醇、异丙醇(IPA)、丙酮)、二甲基亚砜(DMSO)、二甲基甲酰胺(DMF)、乙酸异丙酯、乙酸乙酯、乙酸丁酯、1-辛醇、庚烷、辛烷、甲基叔-丁基醚(MTBE)、甲苯等)、离子液体(例如，四氟硼酸1-乙基4-甲基咪唑、四氟硼酸1-丁基-3-甲基咪唑、六氟磷酸1-丁基-3-甲基咪唑等)。水性共溶剂系统中的有机溶剂组分可与水性组分混溶，提供单一的液相，或可与水性组分部分混溶或不混溶，提供双液相。示例性的水性共溶剂系统包含水和一种或多种有机溶剂。通常，选择水性共溶剂系统的有机溶剂组分以便其不会完全使酮还原酶失活。通过在候选溶剂系统中使用感兴趣的底物并利用诸如本文描述的酶活性测定而测量特定的工程化的酮还原酶的酶活性，合适的共溶剂系统可被容易地鉴定。在方法的一些实施方案中，合适的反应条件包括水性共溶剂，所述水性共溶剂包含约1％至约95％(v/v)、约1％至约60％(v/v)、约2％至约60％(v/v)、约5％至约60％(v/v)、约10％至约60％(v/v)、约10％至约50％(v/v)或约10％至约40％(v/v)的浓度的溶剂。在方法的一些实施方案中，合适的反应条件包括含有至少约1％、5％、10％、15％、20％、25％、30％、35％、40％、45％、50％、55％、60％、65％、70％、75％、80％、85％、90％或95％(v/v)的浓度的溶剂。
合适的反应条件可包括提供将底物化合物生物催化转化成其相应的产物化合物的反应参数的组合。相应地，在方法的一些实施方案中，反应参数的组合包括：(a)约10g/L至600g/L底物A1的载量；(b)约1g/L至50g/L的工程化多肽浓度；(c)约4.0至11.0的pH；和(d)约10℃至60℃的温度。
在一些实施方案中，能够进行上述反应的酮还原酶多肽包括对应SEQ ID NO：4、6、8、10、12、14、16、18、20、22、24、26、28、30、32、34、36、38、40、42、44、46、48、50、52、54、56、58、60、62、64、66、68、70、72、74、76、78、80、82、84、86、88、90、92、94、96、98、100、102、104、106、108、110、112、114、116、118、120、122、124、126、128、130、132、134、136、138、140、142、144、146、148、150、152、154、156、158、160、162、164、166、168、170、172、174、176、178、180、182、184、186、188、190、192、194、196、198、200、202、204、206、208、210、212、214、216、218、220、222、224、226、228、230、232、234、236、238、240、242、244、246、248、250、252、254、256、258、260、262、264、266、268、270、272、274、276、278、280、282、284、286、288、290、292、294、296、298、300、302、304、306、308、310、312、314、316、318、320、322、324、326、328、330、332的氨基酸序列。
示例性反应条件包括在表2、表3以及实施例8-14中提供的条件。
在进行本文描述的酮还原酶催化反应时，工程化的酮还原酶多肽可以部分地纯化的或纯化的酶、经热处理的酶液、用编码酶的基因转化的完整细胞、和/或此类细胞的细胞提取物和/或裂解物的形式加入至反应混合物中。用编码工程化的酮还原酶的基因转化的完整细胞，或其细胞提取物、其裂解物，以及分离的酶可以多种不同的形式使用，包括固体(例如，冻干的、喷雾干燥的等)或半固体(例如，湿菌体等粗糊状物)。细胞提取物或细胞裂解物可通过沉淀(例如，硫酸铵、聚乙烯亚胺、热处理或类似处理)部分纯化，之后在冻干前进行除盐程序(例如，超滤、透析及类似程序)。任何酶制品可通过使用已知的交联剂诸如，例如戊二醛交联或固定到固相材料(例如树脂)而被稳定化。
在本文描述的酮还原酶催化反应的一些实施方案中，反应在本文描述的合适的反应条件下进行，其中工程化酮还原酶多肽被固定至固体支持物上。可用于固定进行酮还原酶催化反应的工程化酮还原酶的固体支持物包括但不限于微球或树脂，所述微球或树脂包含具有环氧官能团的聚甲基丙烯酸酯、具有氨基环氧官能团的聚甲基丙烯酸酯、具有十八烷基官能团的苯乙烯/DVB共聚物或具有十八烷基官能团的聚甲基丙烯酸酯。示例性固体支持物包括但不限于壳聚糖珠、EupergitC和SEPABEAD(Mitsubishi)，包括以下不同类型的SEPABEAD：EC-EP、EC-HFA/S、EXA252、EXE119和EXE120。
在一些实施方案中，其中工程化多肽可以分泌多肽的形式被表达，含有该分泌多肽的培养基可被用于本文的方法中。
在一些实施方案中，固体反应物(例如，酶、盐等)可以各种不同的形式提供给反应，包括粉末(例如，冻干的、喷雾干燥的等)、溶液、乳液、悬浮液等。反应物可使用为本领域普通技术人员共知的方法和仪器容易地冻干或喷雾干燥。例如，蛋白质溶液可以小量冷冻于-80℃，然后加入至预冷却的冻干室内，之后应用真空。
在一些实施方案中，加入反应物的顺序并不关键。反应物可同时一起加入至溶剂中(例如，单相溶剂、双相水性共溶剂体系等)，或者可选择地，一些反应物可分别地加入，以及一些在不同的时间点一起加入。例如，酮还原酶和酮还原酶底物可首先加入溶剂中。然后可加入并混合有机相。可选择地，酮还原酶底物可在添加至水相之前在有机相中预混合。
本公开内容的不同的特征和实施方案示例于以下代表性的实施例中，其意图是例证性的而不是限制性的。
附图说明
图1酮还原酶催化羰基化合物还原为醇类化合物的反应
图2酮还原酶催化4-羟基-2-丁酮转化为(R)-(-)-1,3-丁二醇，并同时催化异丙醇转化为丙酮来实现NADH的循环再生
图3热处理后的酶液SDA-PAGE图
具体实施方式
下面用实施例来进一步说明本发明，但本发明并不受其限制。下列实施例中未注明具体条件的实验方法，通常按照常规条件，或按照制造厂商所建议的条件。
实施例1：基因克隆和表达载体的构建
源自于金黄杆菌CA49(Chryseobacterium sp.CA49)的野生型的ChKRED20酮还原酶的氨基酸序列，可从NCBI上检索得到，然后通过本领域的常见技术将其对应的核酸合成出来并克隆到表达载体pACYC-Duet-1上。将重组表达质粒转化到E.coil BL21(DE3)的感受态细胞中，转化条件42℃，热击90秒，转化液涂布到含有氯霉素的LB平板上，37℃倒置培养过夜，即获得重组转化体。
实施例2：酮还原酶多肽的表达，和含有酮还原酶多肽的酶液的制备
本发明的酶液制作步骤如下：将实施例1所得的重组E.coli BL21(DE3)，接种至装有50mL含氯霉素的LB培养基(蛋白胨10g/L，酵母浸出粉5g/L，氯化钠10g/L，pH值7.0±0.2,25℃)的250mL锥形瓶中，置于30℃，250rpm摇床振荡培养过夜。当培养液的OD600达到2时，按5％(v/v)的接种量接入装有250mL TB培养基(胰蛋白胨12g/L，酵母浸出粉24g/L，磷酸氢二钠9.4g/L，磷酸氢二钾2.2g/L，乳糖6g/L，pH值7.0±0.2,30℃)的1000mL锥形瓶中，置于30℃、250rpm摇床振摇培养，期间乳糖会诱导酮还原酶多肽的表达。20h后，将培养液离心(8000rpm，10分钟)；离心后弃上清液，收集细胞得到湿菌体。将所得的湿菌体悬浮于30mL的磷酸缓冲液中(PBS，pH7.0)，在冰浴中超声破碎，离心(4000rpm，15min)收集上清液，即得含有酮还原酶多肽的酶液。
按照摇瓶制备方式，同比例缩小至96孔板进行培养得到培养液，离心去除上清培养基得到湿菌体，可通过本领域所熟知的化学破碎方法得到酶液。
实施例3：酶液的热处理
由实施例2中所述的制备方法制得SEQ ID No：2和SEQ ID No：330对应的酮还原酶多肽的酶液各25mL。将其分别装入50mL离心管并置于85℃的水浴中，搅动使酶液受热均匀，2小时后，将酶液离心(4000rpm，30min)，收集上清液，即得热处理后的酶液。将热处理后的酶液样品进行SDS-PAGE电泳分析，图3是电泳结果：样品1为SEQ ID No：2对应的热处理后的酶液，样品2为SEQ ID No：330对应的热处理后的酶液。图3的结果显示，经[85℃,2h]热处理后，酶液中的杂蛋白已完全被去除，SEQ ID No：2对应的酮还原酶也被去除，而SEQ ID No：330对应的酮还原酶仍保留在酶液中。
实施例4：酮还原酶突变文库的构建
这里所用到的都是商业试剂，较佳地选用Quikchange试剂盒(供应商：Agilent)。突变引物的序列设计按照试剂盒的说明进行。现以单个残基位置的饱和突变文库的构建为例说明。PCR体系为：5xBuffer 10uL，10mM dNTP 1uL，质粒DNA模板1uL(50ng/uL),上下游引物各取0.75uL(10uM)，高保真酶0.5uL，ddH2O 36uL。PCR引物在突变位置的密码子为NNK。
PCR扩增步骤为：(1)98℃，预变性3min；(2)98℃，变性10s；(3)72℃退火和延伸3min；步骤(2)～(3)重复25次；(5)72℃继续延伸10min，冷却至4℃。在PCR产物中加入2uLDpnI，37℃过夜酶切消除质粒模板。酶切后的PCR产物用电击法转化E.coli BL21(DE3)感受态细胞，涂布到含氯霉素的LB平板上即得目标残基位置的饱和突变文库。
实施例5：酮还原酶突变文库的高通量筛选
对酮还原酶突变文库的表达，按照96孔板进行培养得到菌体，具体操作方案如下：从LB琼脂平板上的突变酶库(如实施例4所述)挑取单克隆，接种至含有氯霉素的LB培养基的96孔平底浅板中(每孔含150μL LB培养基)，置于摇床在180rpm、80％的湿度、30℃过夜培养18～20h。当OD600达到2.0时，取20μL菌液接种到96深孔板中(每孔含400μL TB培养基及6g/L乳糖)，置于250rpm、30℃、80％湿度摇床过夜培养18～20h。之后，将培养过夜的深孔板在4000rpm下离心10min，去除培养基并得到菌体。
接下来对菌体进行破碎，在含有菌体的深孔板中加入200μL/孔的化学破碎液(100mM磷酸缓冲液，pH7.5，含1mg/mL溶菌酶)，封膜后置于平板振荡器上在700rpm下破碎1h，之后离心(4000rpm，10min)，每孔取160μL上清酶液到一个新的孔板中。将含有酶液的孔板封膜，置于水浴摇床72℃下震荡2.5h进行热处理(针对表2或表3中“+”对应的酮还原酶多肽的高通量筛选)。热处理完成后，将酶液在4000rpm下离心15分钟，每个孔取30μL上清到预先装有反应母液(170μL/孔)的96孔板中。然后用铝膜热封96孔板，放置于45℃、200rpm的摇床开始反应。反应15h后，取50μL反应液于一个新的96深孔板，加入1mL乙酸乙酯对反应进行淬灭，置于平板振荡器上震荡30min(800rpm)，然后离心(4000rpm，30min)，取离心后的上清进行GC分析检测转化率。
针对表2或表3中“+”对应的酮还原酶多肽的高通量筛选的反应条件，其反应母液的配制如下：a)将4-羟基-2-丁酮溶解于异丙醇中，b)将NAD+溶解于PBS缓冲液中，将a)、b)与水混合，得到终浓度为【4-羟基-2-丁酮118g/L，异丙醇59％(v/v)，NAD+0.24g/L，PBS0.01M，pH7.0】的反应母液。
实施例6：(R)-(-)-1,3-丁二醇的分析检测方法
GC分析方法：色谱柱为DB-WAX 15m*0.25mm*0.25μm，载气为N2，检测器为FID，进样口温度为250℃，分流比为28：1，检测器温度为300℃，进样量为1μL，柱温为130℃，以10℃/min升温至150℃，后以20℃/min升温至160℃，其中4-羟基-2-丁酮的保留时间为1.5min，(R)-(-)-1,3-丁二醇的保留时间为2.3min。
GC手性分析方法：样品前处理方法：取200μL样品加入50μL的MSTFA与30μL的无水吡啶于1.5mL离心管混合均匀，振荡反应30min。色谱柱为CP-Chirasil Dex CB(CP7502)25m*0.25mm*0.25μm，载气为N2，检测器为FID，进样口温度为250℃，分流比为28：1，检测器温度为300℃，进样量为1μL，柱温为105℃，停止时间为9min，(R)-(-)-1,3-丁二醇的保留时间为6.5min，(S)-(-)-1,3-丁二醇的保留时间为5.82min。
实施例7：发酵及后处理
将包含目标工程化酮还原酶多肽表达质粒的大肠杆菌BL21(DE3)的单个微生物菌落接种到含30μg/mL氯霉素的50mL LB培养基(5.0g/L Yeast Extract、10g/L Tryptone、10g/L氯化钠)中。在30℃摇床中250rpm振动培养16h，当培养液的OD600是3.5至5.0时，从摇床中取出培养液。
将含有0.6L发酵基础培养基的1.0L发酵罐在121℃高压蒸汽灭菌锅灭菌30min。向发酵罐接种上述的摇瓶培养液。控制发酵温度保持在37℃,控制搅拌桨速度在200-1000rpm的范围内，以0.4-0.8L/min向发酵容器供应空气以保持溶解氧水平为35％以上,通过加入25-28％v/v氢氧化铵保持培养液的pH在7.0。菌的生长通过流加含500g/L食用葡萄糖右旋糖一水合物、12g/L氯化铵和5g/L硫酸镁七水合物的补料溶液来维持。培养至8h，当培养液达到OD600为35±5后，将培养液温度下降并维持在30℃，然后加入一水合-α-乳糖至终浓度15g/L来诱导酮还原酶的表达,继续发酵约16小时后放罐收集发酵液。使用ThermoMultifuge X3R离心机，将发酵液在4℃以8000rpm离心10min来收集湿菌体。收集的湿菌体直接用于以下后处理工艺，或可在-20℃冷冻储存直到使用。
在4℃下将湿菌体用10mM pH7.0的磷酸钾盐缓冲液重悬浮,使用ThermoMultifuge X3R离心机在4℃以8000rpm离心10min,再次收集湿菌体，此为清洗后的湿菌体。取上述清洗后湿菌体10g，用50mL 10mM pH7.0磷酸钾盐缓冲液重悬浮,利用压力均质机破碎2次，将酮还原酶从胞内释放。将破碎液在85℃下热处理2h(针对表2或表3中“++++”对应的酮还原酶多肽)，将热处理后的酶液以4000rpm离心30min，收集上清液，得到热处理后的酶液。
实施例8：工程化酮还原酶催化生成(R)-(-)-1,3-丁二醇的反应工艺
以下为一个具代表性的200mL体积的反应流程和后处理工艺。在容积为500mL的反应瓶里，加入80g底物4-羟基-2-丁酮、80mL异丙醇，开启搅拌，然后加入16mL对应于SEQ IDNo：330的热处理后的酶液、4mg辅因子NAD+，最后用纯水将反应瓶中的最终反应体积补足为200mL。反应用水浴控温40℃，搅拌速度200rpm。反应到第12h时,在0.09～0.1Mpa的真空度下使用水泵抽真空6次，以移除反应中产生的丙酮，收集馏分,期间分5次补加异丙醇，每次补加的量为40mL；反应到24h后停止抽真空，反应结束。取样检测反应24小时后的转化率≥99％，ee≥99.5％。
将结束后的反应进行减压蒸馏，收集异丙醇和水，再升温减压精馏收集产物(R)-(-)-1,3-丁二醇。最终得到约64g提纯后的(R)-(-)-1,3-丁二醇，ee≥99.5％。
实施例9：使用葡萄糖脱氢酶来循环再生NADH的反应工艺
以下为一个具代表性的200mL体积的反应流程和后处理工艺。在容积为500mL的反应瓶里，加入20g底物4-羟基-2-丁酮、40mL纯水、20mL对应于SEQ ID No：102的热处理后的酶液、20mL含有葡萄糖脱氢酶的酶液、82g葡萄糖、4mg辅因子NAD+，并用纯水将反应瓶中的最终反应体积补足为200mL。反应用水浴控温40℃，搅拌速度200rpm，反应过程中用1M的NaOH溶液维持体系pH在7.0左右。反应进行到24h后结束。取样检测反应24小时后的转化率约68％，ee≥99.5％。
Figure BDA0001907261180000411
实施例10：比较工程化酮还原酶SEQ ID No：330与野生型酮还原酶SEQ ID No：2在催化生成(R)-(-)-1,3-丁二醇的反应中的催化性能
按照实施例2所述方法，制备a)SEQ ID No：2的酶液和c)SEQ ID No：330的酶液；从a)或c)的酶液拿出一部分经[85℃，2h]热处理后，得到b)热处理后的SEQ ID No：2酶液和d)热处理后的SEQ ID No：330酶液。
取4个容积为250mL的反应瓶，每个反应瓶加入10.0g 4-羟基-2-丁酮和25mL异丙醇，然后在4个反应瓶中分别加入4种不同的酶液：a)15mL、b)15mL、c)4mL、d)4mL，然后每个反应瓶中加入0.2mL的NAD+辅因子的母液(12.5g/L)，并用纯水将每个反应瓶中的最终反应体积补足为50mL,这样每个反应瓶中的底物终浓度为200g/L、异丙醇的终浓度为50％v/v，NAD+的终浓度为0.05g/L。反应用水浴控温40℃，搅拌速度200rpm。反应到第12h时,在0.09～0.1Mpa的真空度下使用水泵抽真空，以移除反应中产生的丙酮，同时补加异丙醇，维持反应体系中异丙醇的浓度为50％v/v；反应到24h后停止抽真空，反应结束,从每个反应瓶取50μL反应液样品，加入1mL乙酸乙酯对样品进行淬灭，然后按照实施例6的分析方法检测并计算转化率和ee值，所得结果见表4。
表4
酶液 酶液载量 转化率 ee
a)未经热处理的SEQ ID No：2酶液 30％v/v 44.9％ ＞99％
b)[85℃，2h]热处理后的SEQ ID No：2酶液 30％v/v 0 -
c)未经热处理的SEQ ID No：330酶液 8％v/v 99.1％ ＞99％
d)[85℃，2h]热处理后的SEQ ID No：330酶液 8％v/v 96.8％ ＞99％
实施例11：酮还原酶催化生成(R)-(-)-3-羟基丁酸甲酯
取4个容积为30mL的反应瓶，每个反应瓶加入1.0g乙酰乙酸甲酯和2.0mL异丙醇，然后在4个反应瓶中分别加入4种不同的酶液：e)0.25mL的未经热处理的SEQ ID No：2酶液、f)0.25mL的[72℃、2.5h]热处理后的SEQ ID No：2酶液、g)0.1mL的未经热处理的SEQ IDNo：188酶液、h)0.1mL的[80℃，2h]热处理后的SEQ ID No：188酶液，然后每个反应瓶中加入0.04mL的NAD+辅因子的母液(12.5g/L)，并用纯水将每个反应瓶中的最终反应体积补足为5.0mL,这样每个反应瓶中的底物(乙酰乙酸甲酯)的终浓度为200g/L、异丙醇的终浓度为40％v/v，NAD+的终浓度为0.1g/L。将反应瓶放在40℃的IKA磁力搅拌器上，搅拌速度设置为400rpm，开始反应。反应进行24h后，从每个反应瓶取样处理并进行检测，计算转化率和ee值，所得结果见表5。
表5
酶液 酶液载量 转化率 ee
e)未经热处理的SEQ ID No：2酶液 5％v/v 84.7％ ＞99％
f)[72℃、2.5h]热处理后的SEQ ID No：2酶液 5％v/v 40.5％ ＞99％
g)未经热处理的SEQ ID No：188酶液 2％v/v 98.8％ ＞99％
h)[80℃，2h]热处理后的SEQ ID No：188酶液 2％v/v 97.0％ ＞99％
分析方法：
GC转化率分析方法：色谱柱为CP-ChiraSil-DEX CB 25m*0.25mm*0.25μm，载气为N2，检测器为FID，进样口温度为250℃，分流比为100：1，检测器温度为250℃，进样量为1μL，柱温为120℃恒温5min，其中乙酰乙酸甲酯的保留时间为2.7min，(R)-(-)-3-羟基丁酸甲酯的保留时间为3.1min。
GC手性分析方法：样品前处理方法：取200μL稀释后的样品加入50μL的MSTFA与30μL的无水吡啶于1.5mL离心管混合均匀，振荡反应30min。色谱柱为CP-Chirasil Dex CB(CP7502)25m*0.25mm*0.25μm，载气为N2，检测器为FID，进样口温度为250℃，分流比为50：1，检测器温度为250℃，进样量为1μL，柱温为110℃，停止时间为8min，(R)-(-)-3-羟基丁酸甲酯的保留时间为3.7min，(S)-(-)-3-羟基丁酸甲酯的保留时间为3.9min。
实施例12：酮还原酶催化生成(R)-(-)-3-羟基丁酸乙酯
取4个容积为30mL的反应瓶，每个反应瓶加入1.0g乙酰乙酸乙酯和2.0mL异丙醇，然后在4个反应瓶中分别加入4种不同的酶液：i)0.25mL的未经热处理的SEQ ID No：2酶液、j)0.25mL的[72℃、2.5h]热处理后的SEQ ID No：2酶液、k)0.1mL的未经热处理的SEQ IDNo：240酶液、l)0.1mL的[85℃，2h]热处理后的SEQ ID No：240酶液，然后每个反应瓶中加入0.04mL的NAD+辅因子的母液(12.5g/L)，并用纯水将每个反应瓶中的最终反应体积补足为5.0mL,这样每个反应瓶中的底物(乙酰乙酸乙酯)的终浓度为200g/L、异丙醇的终浓度为40％v/v，NAD+的终浓度为0.1g/L。将反应瓶放在40℃的IKA磁力搅拌器上，搅拌速度设置为400rpm，开始反应。反应进行24h后，从每个反应瓶取样处理并进行检测，计算转化率和ee值，所得结果见表6。
表6
Figure BDA0001907261180000431
Figure BDA0001907261180000441
分析方法：
GC转化率分析方法：色谱柱为CP-ChiraSil-DEX CB 25m*0.25mm*0.25μm，载气为N2，检测器为FID，进样口温度为250℃，分流比为50：1，检测器温度为250℃，进样量为1μL，柱温为120℃恒温4min，其中乙酰乙酸乙酯的保留时间为3.2min，(R)-(-)-3-羟基丁酸乙酯的保留时间为3.7min。
GC手性分析方法：色谱柱为CP-Chirasil Dex CB(CP7502)25m*0.25mm*0.25μm，载气为N2，检测器为FID，进样口温度为250℃，分流比为100：1，检测器温度为250℃，进样量为1μL，柱温为100℃，停止时间为15min，(R)-(-)-3-羟基丁酸乙酯的保留时间为10.3min，(S)-(-)-3-羟基丁酸乙酯的保留时间为10.8min。
实施例13：酮还原酶催化生成(S)-(-)-4-氯-3-羟基丁酸乙酯
取4个容积为30mL的反应瓶，每个反应瓶加入0.3g 4-氯乙酰乙酸乙酯、2.0mL异丙醇和2.6mL三乙醇胺缓冲液(0.1M pH＝8.5)，然后在4个反应瓶中分别加入4种不同的酶液：m)0.25mL的未经热处理的SEQ ID No：2酶液、n)0.25mL的[72℃、2.5h]热处理后的SEQ IDNo：2酶液、o)0.05mL的未经热处理的SEQ ID No：332酶液、p)0.05mL的[85℃，2h]热处理后的SEQ ID No：332酶液，然后每个反应瓶中加入0.04mL的NAD+辅因子的母液(12.5g/L)，并用纯水将每个反应瓶中的最终反应体积补足为5.0mL,这样每个反应瓶中的底物(4-氯乙酰乙酸乙酯)的终浓度为60g/L、异丙醇的终浓度为40％v/v，NAD+的终浓度为0.1g/L。将反应瓶放在40℃的IKA磁力搅拌器上，搅拌速度设置为400rpm，开始反应。反应进行24h后，从每个反应瓶取样处理并进行检测，计算转化率和ee值，所得结果见表7。
表7
Figure BDA0001907261180000442
Figure BDA0001907261180000451
分析方法：
GC转化率分析方法：色谱柱为CP-ChiraSil-DEX CB 25m*0.25mm*0.25μm，载气为N2，检测器为FID，进样口温度为250℃，分流比为98：1，检测器温度为250℃，进样量为1μL，柱温为50℃以30℃/min升温至180℃，其中4-氯乙酰乙酸乙酯的保留时间为10.4min，(S)-(-)-4-氯-3-羟基丁酸乙酯的保留时间为9.9min。
GC手性分析方法：色谱柱为CP-Chirasil Dex CB(CP7502)25m*0.25mm*0.25μm，载气为N2，检测器为FID，进样口温度为250℃，分流比为100：1，检测器温度为250℃，进样量为1μL，柱温为105℃，停止时间为15min，(S)-(-)-4-氯-3-羟基丁酸乙酯的保留时间为11.5min，(R)-(-)-4-氯-3-羟基丁酸乙酯的保留时间为11.9min。
实施例14：酮还原酶催化生成(R)-1-[3,5-二(三氟甲基)苯基]乙醇
取容积为30mL的反应瓶，加入1.0g的3，5-双三氟甲基苯乙酮、1.0mL异丙醇和0.08mL的NAD+辅因子的母液(12.5g/L)，然后加入0.5mL的[75℃、2h]热处理后的SEQ IDNo：142酶液，并用纯水将反应瓶中的最终反应体积补足为5.0mL,这样反应瓶中的底物(3，5-双三氟甲基苯乙酮)的终浓度为200g/L、异丙醇的终浓度为20％v/v，NAD+的终浓度为0.2g/L。将反应瓶放在40℃的IKA磁力搅拌器上，搅拌速度设置为400rpm，开始反应。反应进行24h后，转化率＞95％，产物(R)-1-[3,5-二(三氟甲基)苯基]乙醇的ee≥99.9％。
分析方法：
HPLC转化率分析方法：色谱柱:ZORBAX SB-C18、流动相：60％乙腈+40％水、柱温：30℃、进样量：10μL、流速：1ml/min、波长：218nm、其中(R)-1-[3,5-二(三氟甲基)苯基]乙醇的保留时间为4.9min，3，5-双三氟甲基苯乙酮的保留时间为6.7min。
GC手性分析方法：色谱柱：Beta DEXTM 225(30m*0.25mm*0.25μm)、柱温：110℃恒温持续20min、柱流量：1.0ml/min、气化室：250℃、检测器：FID 330℃、分流比：1:30、进样量：1.0μl。其中(S)-1-[3,5-二(三氟甲基)苯基]乙醇的保留时间为15.77min，(R)-1-[3,5-二(三氟甲基)苯基]乙醇的保留时间为16.33min。
实施例15：酮还原酶拆分外消旋的1,3-丁二醇的反应工艺
以下为一个具代表性的200mL体积的反应流程和后处理工艺，以外消旋的1,3-丁二醇为底物(含等量的(R)-(-)-1,3-丁二醇和(S)-(-)-1,3-丁二醇)。在容积为500mL的反应瓶里，加入10g外消旋的1,3-丁二醇、20g丙酮、20mL对应于SEQ ID No：330的[85℃，2h]热处理后的酶液、4mg辅因子NAD+，并用纯水将反应瓶中的最终反应体积补足为200mL。反应用水浴控温40℃，搅拌速度200rpm。24h后反应结束，取样检测，底物中≥99.5％的(R)-(-)-1,3-丁二醇转化成了4-羟基-2-丁酮，(S)-(-)-1,3-丁二醇的ee≥99％。
应理解，在阅读了本发明的上述内容之后，本领域技术人员可以对本发明作各种改动或修改，这些等价形式同样落于本申请所附权利要求书所限定的范围。
序列表
<110> 宁波酶赛生物工程有限公司
<120> 工程化酮还原酶多肽及其应用
<130> EMP059
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cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgggcccggc ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 6
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 6
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Ala Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Ile Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Lys Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Ala Pro Leu Ser Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Gly Pro Ala Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 7
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 7
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaagcggt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatatcgc atgcaacaat gctggcattg gcggtgaaca ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcggcc ccgctgaact ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgggcccggc ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 8
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 8
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Ala Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Ile Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Gln Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Ala Pro Leu Asn Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Gly Pro Ala Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 9
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 9
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaagcggt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatatcgc atgcaacaat gctggcattg gcggtgaaca ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcgtac ccgctgagct ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgggcccggc ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 10
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 10
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Ala Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Ile Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Gln Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Tyr Pro Leu Ser Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Gly Pro Ala Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 11
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 11
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaagcggt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatatcgc atgcaacaat gctggcattg gcggtgaaca ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcgcgc ccgctgagct ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgggcccggc ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 12
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 12
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Ala Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Ile Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Gln Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Arg Pro Leu Ser Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Gly Pro Ala Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 13
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 13
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaagcggt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatatcgc atgcaacaat gctggcattg gcggtgaacg cgcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcggcc ccgctgagct ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgggcccggc ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 14
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 14
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Ala Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Ile Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Arg Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Ala Pro Leu Ser Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Gly Pro Ala Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 15
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 15
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaagcggt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatatcgc atgcaacaat gctggcatta ggggtgaaca ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcggcc ccgctgagct ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgggcccggc ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 16
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 16
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Ala Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Ile Ala Cys Asn Asn Ala Gly Ile Arg Gly Glu
85 90 95
Gln Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Ala Pro Leu Ser Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Gly Pro Ala Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 17
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 17
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaagcggt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatatcgc atgcaacaat gctggcattc tgggtgaaca ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcggcc ccgctgagct ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgggcccggc ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 18
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 18
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Ala Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Ile Ala Cys Asn Asn Ala Gly Ile Leu Gly Glu
85 90 95
Gln Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Ala Pro Leu Ser Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Gly Pro Ala Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 19
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 19
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaagcggt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatatcgc atgcaacaat gctggcattg ttggtgaaca ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcggcc ccgctgagct ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgggcccggc ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 20
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 20
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Ala Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Ile Ala Cys Asn Asn Ala Gly Ile Val Gly Glu
85 90 95
Gln Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Ala Pro Leu Ser Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Gly Pro Ala Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 21
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 21
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaagcggt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatatcgc atgcaacaat gctggcattc agggtgaaca ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcggcc ccgctgagct ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgggcccggc ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 22
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 22
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Ala Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Ile Ala Cys Asn Asn Ala Gly Ile Gln Gly Glu
85 90 95
Gln Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Ala Pro Leu Ser Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Gly Pro Ala Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 23
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 23
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaagcggt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatatcgc atgcaacaat gctggcatta agggtgaaca ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcggcc ccgctgagct ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgggcccggc ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 24
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 24
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Ala Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Ile Ala Cys Asn Asn Ala Gly Ile Lys Gly Glu
85 90 95
Gln Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Ala Pro Leu Ser Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Gly Pro Ala Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 25
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 25
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaagcggt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatatcgc atgcaacaat gctggcattg gcggtgaaca ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcgaag ccgctgagct ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgggcccggc ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 26
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 26
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Ala Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Ile Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Gln Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Lys Pro Leu Ser Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Gly Pro Ala Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 27
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 27
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaagcggt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatatcgc atgcaacaat gctggcattg gcggtgaaca ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcggcc ccgctgatgt ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgggcccggc ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 28
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 28
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Ala Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Ile Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Gln Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Ala Pro Leu Met Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Gly Pro Ala Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 29
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 29
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaagcggt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatatcgc atgcaacaat gctggcattg gcggtgaaca ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcggcc ccgctgttgt ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgggcccggc ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 30
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 30
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Ala Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Ile Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Gln Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Ala Pro Leu Leu Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Gly Pro Ala Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 31
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 31
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaagcggt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatatcgc atgcaacaat gctggcattg gcggtgaaca ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcggcc ccgctgcagt ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgggcccggc ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 32
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 32
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Ala Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Ile Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Gln Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Ala Pro Leu Gln Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Gly Pro Ala Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 33
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 33
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaagcggt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatatcgc atgcaacaat gctggcattg gcggtgaaca ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcggcc ccgtatagct ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgggcccggc ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 34
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 34
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Ala Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Ile Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Gln Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Ala Pro Tyr Ser Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Gly Pro Ala Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 35
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 35
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaagtggt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaca ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcggcc ccgctgagct ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 36
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 36
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Val Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Gln Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Ala Pro Leu Ser Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 37
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 37
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaagtggt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaca ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcggcc ccgctgagct ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct ggcgtccgaa aaatcatcgt tcatgaccgg tggcgcgtac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 38
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 38
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Val Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Gln Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Ala Pro Leu Ser Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ala Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Ala Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 39
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 39
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatatcgc atgcaacaat gctggcattg gcggtgaaca ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcggcc ccgctgagct ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgggcccggc ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 40
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 40
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Ile Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Gln Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Ala Pro Leu Ser Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Gly Pro Ala Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 41
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 41
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatatcgc atgcaacaat gctggcattg gcggtgaaca ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcggcc ccgctgagct ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcgtgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 42
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 42
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Ile Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Gln Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Ala Pro Leu Ser Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Val Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 43
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 43
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaagcggt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatatcgc atgcaacaat gctggcattg gcggtgaaca ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcggcc ccgctgagct ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct ggcgtccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 44
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 44
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Ala Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Ile Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Gln Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Ala Pro Leu Ser Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ala Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 45
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 45
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatatcgc atgcaacaat gctggcattg gcggtgaaca ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcggcc ccgctgagct ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 46
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 46
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Ile Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Gln Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Ala Pro Leu Ser Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 47
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 47
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaagcggt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatatcgc atgcaacaat gctggcattg gcggtgaaca ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcggcc ccgctgagct ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgggcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct ggcgtccgaa aaatcatcgt tcatcaccgg tggcgcgtac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 48
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 48
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Ala Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Ile Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Gln Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Ala Pro Leu Ser Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Gly Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ala Ser Glu Lys Ser Ser Phe Ile Thr Gly Gly Ala Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 49
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 49
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatatcgc atgcaacaat gctggcattg gcggtgaaca ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcggcc ccgctgagct ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct ggcgtccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 50
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 50
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Ile Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Gln Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Ala Pro Leu Ser Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ala Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 51
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 51
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaagcggt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatatcgc atgcaacaat gctggcattg gcggtgaaca ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcggcc ccgctgagct ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggcgcgtac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 52
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 52
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Ala Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Ile Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Gln Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Ala Pro Leu Ser Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Ala Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 53
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 53
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaagcggt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaca ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcggcc ccgctgagct ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct ggcgtccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 54
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 54
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Ala Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Gln Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Ala Pro Leu Ser Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ala Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 55
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 55
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaagtggt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatatcgc atgcaacaat gctggcattg gcggtgaaca ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcggcc ccgctgagct ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggcgcgtac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 56
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 56
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Val Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Ile Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Gln Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Ala Pro Leu Ser Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Ala Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 57
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 57
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaagtggt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaca ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcggcc ccgctgagct ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggc ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 58
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 58
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Val Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Gln Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Ala Pro Leu Ser Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Ala Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 59
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 59
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaca ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcggcc ccgctgagct ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct ggcgtccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 60
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 60
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Gln Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Ala Pro Leu Ser Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ala Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 61
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 61
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaagtggt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatatcgc atgcaacaat gctggcattg gcggtgaaca ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcggcc ccgctgagct ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggc ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 62
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 62
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Val Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Ile Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Gln Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Ala Pro Leu Ser Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Ala Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 63
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 63
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaagtggt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaca ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcggcc ccgctgagct ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgggcccggc ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggcgcgtac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 64
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 64
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Val Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Gln Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Ala Pro Leu Ser Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Gly Pro Ala Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Ala Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 65
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 65
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatatcgc atgcaacaat gctggcattg gcggtgaaca ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcggcc ccgctgagct ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggc ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 66
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 66
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Ile Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Gln Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Ala Pro Leu Ser Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Ala Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 67
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 67
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatatcgc atgcaacaat gctggcattg gcggtgaaca ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcggcc ccgctgagct ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgggcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 68
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 68
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Ile Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Gln Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Ala Pro Leu Ser Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Gly Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 69
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 69
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaagtggt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatatcgc atgcaacaat gctggcattg gcggtgaaca ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcggcc ccgctgagct ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct ggcgtccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 70
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 70
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Val Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Ile Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Gln Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Ala Pro Leu Ser Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ala Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 71
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 71
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaagcggt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaca ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcggcc ccgctgagct ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgggcccggc ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct ggcgtccgaa aaatcatcgt tcatgaccgg tggcgcgtac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 72
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 72
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Ala Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Gln Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Ala Pro Leu Ser Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Gly Pro Ala Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ala Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Ala Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 73
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 73
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaagcggt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaca ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcggcc ccgctgagct ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggcgcgtac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 74
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 74
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Ala Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Gln Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Ala Pro Leu Ser Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Ala Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 75
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 75
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaagtggt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatatcgc atgcaacaat gctggcattg gcggtgaaca ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcggcc ccgctgagct ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgggcccggc ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 76
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 76
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Val Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Ile Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Gln Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Ala Pro Leu Ser Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Gly Pro Ala Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 77
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 77
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaagtggt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatatcgc atgcaacaat gctggcattg gcggtgaaca ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcggcc ccgctgagct ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggc ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct ggcgtccgaa aaatcatcgt tcatcaccgg tggcgcgtac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 78
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 78
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Val Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Ile Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Gln Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Ala Pro Leu Ser Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Ala Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ala Ser Glu Lys Ser Ser Phe Ile Thr Gly Gly Ala Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 79
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 79
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaagcggt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaca ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcggcc ccgctgagct ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 80
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 80
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Ala Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Gln Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Ala Pro Leu Ser Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 81
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 81
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaca ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcggcc ccgctgagct ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggc ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct ggcgtccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 82
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 82
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Gln Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Ala Pro Leu Ser Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Ala Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ala Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 83
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 83
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaagtggt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatatcgc atgcaacaat gctggcattg gcggtgaaca ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcggcc ccgctgagct ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 84
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 84
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Val Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Ile Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Gln Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Ala Pro Leu Ser Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 85
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 85
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaagtggt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatatcgc atgcaacaat gctggcattg gcggtgaaca ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcggcc ccgctgagct ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgggcccggc ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct ggcgtccgaa aaatcatcgt tcatcaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 86
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 86
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Val Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Ile Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Gln Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Ala Pro Leu Ser Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Gly Pro Ala Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ala Ser Glu Lys Ser Ser Phe Ile Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 87
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 87
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaca ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcggcc ccgctgagct ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggc ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct ggcgtccgaa aaatcatcgt tcatgaccgg tggcgcgtac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 88
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 88
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Gln Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Ala Pro Leu Ser Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Ala Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ala Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Ala Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 89
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 89
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaagcggt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatatcgc atgcaacaat gctggcattg gcggtgaaca ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcggcc ccgctgagct ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 90
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 90
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Ala Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Ile Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Gln Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Ala Pro Leu Ser Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 91
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 91
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaagcggt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatatcgc atgcaacaat gctggcattg gcggtgaaca ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcggcc ccgctgagct ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgggcccggc ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct ggcgtccgaa aaatcatcgt tcatcaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 92
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 92
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Ala Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Ile Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Gln Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Ala Pro Leu Ser Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Gly Pro Ala Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ala Ser Glu Lys Ser Ser Phe Ile Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 93
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 93
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaagtggt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatatcgc atgcaacaat gctggcattg gcggtgaaca ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcggcc ccgctgagct ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggc ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggcgcgtac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 94
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 94
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Val Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Ile Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Gln Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Ala Pro Leu Ser Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Ala Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Ala Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 95
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 95
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaagtggt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatatcgc atgcaacaat gctggcattg gcggtgaaca ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcggcc ccgctgagct ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct ggcgtccgaa aaatcatcgt tcatgaccgg tggcgcgtac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 96
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 96
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Val Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Ile Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Gln Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Ala Pro Leu Ser Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ala Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Ala Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 97
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 97
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaagcggt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatatcgc atgcaacaat gctggcattg gcggtgaaca ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcggcc ccgctgagct ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgggcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct ggcgtccgaa aaatcatcgt tcatcaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 98
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 98
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Ala Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Ile Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Gln Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Ala Pro Leu Ser Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Gly Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ala Ser Glu Lys Ser Ser Phe Ile Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 99
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 99
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatatcgc atgcaacaat gctggcattg gcggtgaaca ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcggcc ccgctgagct ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggc ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct ggcgtccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 100
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 100
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Ile Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Gln Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Ala Pro Leu Ser Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Ala Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ala Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 101
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 101
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaca ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcggcc ccgctgagct ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 102
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 102
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Gln Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Ala Pro Leu Ser Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 103
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 103
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaagtggt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatatcgc atgcaacaat gctggcattg gcggtgaaca ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcggcc ccgctgagct ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct ggcgtccgaa aaatcatcgt tcgtgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 104
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 104
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Val Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Ile Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Gln Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Ala Pro Leu Ser Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ala Ser Glu Lys Ser Ser Phe Val Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 105
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 105
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaagtggt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaca ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcggcc ccgctgagct ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcgtgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 106
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 106
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Val Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Gln Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Ala Pro Leu Ser Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Val Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 107
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 107
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaagtggt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaca ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcggcc ccgctgagct ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct ggcgtccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 108
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 108
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Val Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Gln Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Ala Pro Leu Ser Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ala Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 109
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 109
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggtagggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaagcggt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatatcgc atgcaacaat gctggcattg gcggtgaaca ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcggcc ccgctgagct ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgggcccggc ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 110
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 110
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Arg
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Ala Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Ile Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Gln Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Ala Pro Leu Ser Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Gly Pro Ala Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 111
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 111
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaagcggt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccgctaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatatcgc atgcaacaat gctggcattg gcggtgaaca ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcggcc ccgctgagct ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgggcccggc ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 112
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 112
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Ala Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ala Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Ile Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Gln Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Ala Pro Leu Ser Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Gly Pro Ala Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 113
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 113
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaagcggt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatatcgc atgcaacaat gctggcattg gcggtgaaca ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtctg tgaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcggcc ccgctgagct ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgggcccggc ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 114
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 114
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Ala Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Ile Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Gln Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Val Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Ala Pro Leu Ser Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Gly Pro Ala Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 115
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 115
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaagcggt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatatcgc atgcaacaat gctggcattg gcggtgaaca ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atcgccagca ttcatggtat cgttgcggcc ccgctgagct ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgggcccggc ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 116
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 116
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Ala Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Ile Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Gln Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Ile Ala Ser Ile
130 135 140
His Gly Ile Val Ala Ala Pro Leu Ser Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Gly Pro Ala Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 117
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 117
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggtaaggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaagcggt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatatcgc atgcaacaat gctggcattg gcggtgaaca ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcggcc ccgctgagct ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgggcccggc ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 118
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 118
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Lys
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Ala Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Ile Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Gln Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Ala Pro Leu Ser Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Gly Pro Ala Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 119
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 119
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga ttgcaacgaa 120
gaccatggta ataaagcggt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatatcgc atgcaacaat gctggcattg gcggtgaaca ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcggcc ccgctgagct ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgggcccggc ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 120
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 120
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Cys Asn Glu Asp His Gly Asn Lys Ala Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Ile Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Gln Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Ala Pro Leu Ser Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Gly Pro Ala Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 121
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 121
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga ttggaacgaa 120
gaccatggta ataaagcggt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatatcgc atgcaacaat gctggcattg gcggtgaaca ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcggcc ccgctgagct ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgggcccggc ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 122
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 122
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Trp Asn Glu Asp His Gly Asn Lys Ala Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Ile Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Gln Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Ala Pro Leu Ser Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Gly Pro Ala Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 123
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 123
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggtaacgg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaagcggt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatatcgc atgcaacaat gctggcattg gcggtgaaca ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcggcc ccgctgagct ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgggcccggc ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 124
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 124
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Asn
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Ala Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Ile Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Gln Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Ala Pro Leu Ser Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Gly Pro Ala Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 125
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 125
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaaa ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcgaag ccgctgagct ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 126
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 126
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Lys Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Lys Pro Leu Ser Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 127
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 127
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaaa ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcggcc ccgctgcgct ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 128
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 128
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Lys Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Ala Pro Leu Arg Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 129
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 129
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaca ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcgcag ccgctgaact ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 130
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 130
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Gln Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Gln Pro Leu Asn Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 131
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 131
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaaa ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcgcgc ccgctgagct ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 132
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 132
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Lys Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Arg Pro Leu Ser Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 133
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 133
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaca ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcgaag ccgctgagct ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 134
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 134
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Gln Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Lys Pro Leu Ser Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 135
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 135
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaacg cgcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcgaac ccgctgcagt ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 136
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 136
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Arg Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Asn Pro Leu Gln Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 137
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 137
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaacg cgcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcggcc ccgctgcagt ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 138
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 138
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Arg Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Ala Pro Leu Gln Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 139
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 139
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaaa ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcggcc ccgctgcagt ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 140
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 140
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Lys Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Ala Pro Leu Gln Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 141
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 141
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaaa ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcgcag ccgctgaact ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 142
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 142
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Lys Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Gln Pro Leu Asn Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 143
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 143
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaca ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcggcc ccgctgcagt ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 144
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 144
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Gln Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Ala Pro Leu Gln Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 145
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 145
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaca ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcgaag ccgctgaact ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 146
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 146
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Gln Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Lys Pro Leu Asn Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 147
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 147
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaca ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcggcc ccgctgagct ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tggcgccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctcttac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 148
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 148
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Gln Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Ala Pro Leu Ser Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Ala Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Ser Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 149
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 149
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaca ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcggcc ccgctgagct ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tggcgccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggcgattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 150
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 150
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Gln Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Ala Pro Leu Ser Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Ala Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Asp Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 151
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 151
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaca ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcggcc ccgctgagct ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tggcgccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggcacttac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 152
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 152
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Gln Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Ala Pro Leu Ser Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Ala Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Thr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 153
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 153
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaca ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcggcc ccgctgagct ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tggcgccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggcccgtac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 154
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 154
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Gln Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Ala Pro Leu Ser Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Ala Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Pro Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 155
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 155
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gacccgggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaca ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcggcc ccgctgagct ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 156
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 156
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp Pro Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Gln Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Ala Pro Leu Ser Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 157
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 157
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tgtgaacgaa 120
gacaagggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaca ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcggcc ccgctgagct ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 158
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 158
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Val Asn Glu Asp Lys Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Gln Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Ala Pro Leu Ser Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 159
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 159
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccgcggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaca ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcggcc ccgctgagct ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 160
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 160
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp Arg Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Gln Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Ala Pro Leu Ser Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 161
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 161
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gacaccggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaca ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcggcc ccgctgagct ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 162
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 162
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp Thr Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Gln Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Ala Pro Leu Ser Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 163
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 163
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcagcgaa 120
gacaagggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaca ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcggcc ccgctgagct ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 164
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 164
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Ser Glu Asp Lys Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Gln Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Ala Pro Leu Ser Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 165
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 165
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tcgcaacgaa 120
gaccatggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaca ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcggcc ccgctgagct ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 166
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 166
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Arg Asn Glu Asp His Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Gln Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Ala Pro Leu Ser Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 167
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 167
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gacaagggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaca ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcggcc ccgctgagct ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 168
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 168
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp Lys Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Gln Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Ala Pro Leu Ser Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 169
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 169
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tcgcaacgaa 120
gacaccggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaca ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcggcc ccgctgagct ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 170
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 170
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Arg Asn Glu Asp Thr Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Gln Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Ala Pro Leu Ser Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 171
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 171
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tcgcaacgaa 120
gacagcggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaca ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcggcc ccgctgagct ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 172
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 172
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Arg Asn Glu Asp Ser Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Gln Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Ala Pro Leu Ser Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 173
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 173
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcagcgaa 120
gaccatggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaca ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcggcc ccgctgagct ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 174
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 174
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Ser Glu Asp His Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Gln Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Ala Pro Leu Ser Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 175
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 175
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaca ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
aacgccagca ttcatggtat cgttgcggcc ccgctgagct ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 176
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 176
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Gln Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Asn Ala Ser Ile
130 135 140
His Gly Ile Val Ala Ala Pro Leu Ser Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 177
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 177
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg cgtgggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaca ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcggcc ccgctgagct ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 178
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 178
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Val Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Gln Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Ala Pro Leu Ser Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 179
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 179
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaca ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atcgccagca ttcatggtat cgttgcggcc ccgctgagct ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 180
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 180
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Gln Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Ile Ala Ser Ile
130 135 140
His Gly Ile Val Ala Ala Pro Leu Ser Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 181
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 181
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaca ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
accgccagca ttcatggtat cgttgcggcc ccgctgagct ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 182
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 182
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Gln Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Thr Ala Ser Ile
130 135 140
His Gly Ile Val Ala Ala Pro Leu Ser Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 183
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 183
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gacaagggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccgcgaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattc tgggtgaaaa ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtctg tgaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcgcag ccgctgaact ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tggcgccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 184
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 184
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp Lys Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ala Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Leu Gly Glu
85 90 95
Lys Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Val Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Gln Pro Leu Asn Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Ala Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 185
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 185
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccgcgaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaaa ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcgcag ccgctgaact ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 186
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 186
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ala Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Lys Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Gln Pro Leu Asn Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 187
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 187
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccgcggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaaa ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtctg tgaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcgcag ccgctgaact ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 188
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 188
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp Arg Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Lys Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Val Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Gln Pro Leu Asn Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 189
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 189
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gacaagggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccgcgaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattc tgggtgaaaa ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
aacgccagca ttcatggtat cgttgcgcag ccgctgaact ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 190
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 190
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp Lys Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ala Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Leu Gly Glu
85 90 95
Lys Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Asn Ala Ser Ile
130 135 140
His Gly Ile Val Ala Gln Pro Leu Asn Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 191
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 191
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccgcggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccgcgaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaaa ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtctg tgaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcgcag ccgctgaact ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggcccgtac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 192
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 192
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp Arg Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ala Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Lys Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Val Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Gln Pro Leu Asn Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Pro Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 193
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 193
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gacaagggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaaa ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtctg tgaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcgcag ccgctgaact ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 194
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 194
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp Lys Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Lys Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Val Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Gln Pro Leu Asn Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 195
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 195
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gacaagggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccgcgaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaaa ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcgcag ccgctgaact ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac taaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 196
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 196
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp Lys Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ala Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Lys Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Gln Pro Leu Asn Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 197
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 197
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccgcggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccgcgaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattc tgggtgaaaa ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtctg tgaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcgcag ccgctgaact ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggcccgtac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 198
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 198
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp Arg Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ala Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Leu Gly Glu
85 90 95
Lys Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Val Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Gln Pro Leu Asn Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Pro Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 199
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 199
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccgcggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccgcgaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattc tgggtgaaaa ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtctg tgaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
aacgccagca ttcatggtat cgttgcgcag ccgctgaact ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 200
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 200
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp Arg Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ala Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Leu Gly Glu
85 90 95
Lys Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Val Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Asn Ala Ser Ile
130 135 140
His Gly Ile Val Ala Gln Pro Leu Asn Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 201
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 201
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccgcggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca tcagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattc ttggtgaaaa ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtctg tgaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcgcag ccgctgaact ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggcccgtac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 202
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 202
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp Arg Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Ile
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Leu Gly Glu
85 90 95
Lys Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Val Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Gln Pro Leu Asn Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Pro Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 203
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 203
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccgcggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaaa ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtctg tgaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcgcag ccgctgaact ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggcccgtac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 204
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 204
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp Arg Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Lys Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Val Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Gln Pro Leu Asn Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Pro Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 205
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 205
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gacaagggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattc tgggtgaaaa ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtctg tgaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcgcag ccgctgaact ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 206
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 206
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp Lys Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Leu Gly Glu
85 90 95
Lys Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Val Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Gln Pro Leu Asn Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 207
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 207
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccgcggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattc tgggtgaaaa ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtctg tgaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcgcag ccgctgaact ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 208
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 208
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp Arg Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Leu Gly Glu
85 90 95
Lys Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Val Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Gln Pro Leu Asn Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 209
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 209
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gacaagggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccgcgaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattc tgggtgaaaa ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcgcag ccgctgaact ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 210
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 210
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp Lys Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ala Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Leu Gly Glu
85 90 95
Lys Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Gln Pro Leu Asn Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 211
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 211
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccgcggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccgcgaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaaa ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtctg tgaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcgcag ccgctgaact ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 212
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 212
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp Arg Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ala Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Lys Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Val Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Gln Pro Leu Asn Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 213
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 213
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccgcggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattc tgggtgaaaa ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtctg tgaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcgcag ccgctgaact ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggcccgtac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 214
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 214
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp Arg Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Leu Gly Glu
85 90 95
Lys Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Val Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Gln Pro Leu Asn Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Pro Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 215
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 215
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaaa ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtctg tgaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcgcag ccgctgaact ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 216
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 216
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Lys Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Val Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Gln Pro Leu Asn Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 217
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 217
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccgcgaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaaa ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtctg tgaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcgcag ccgctgaact ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 218
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 218
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ala Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Lys Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Val Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Gln Pro Leu Asn Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 219
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 219
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaaa ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta gcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcgcag ccgctgaact ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 220
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 220
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Lys Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ser Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Gln Pro Leu Asn Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 221
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 221
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccaccaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaaa ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcgcag ccgctgaact ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 222
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 222
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Thr Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Lys Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Gln Pro Leu Asn Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 223
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 223
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg cgggtgaaaa ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcgcag ccgctgaact ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 224
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 224
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Ala Gly Glu
85 90 95
Lys Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Gln Pro Leu Asn Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 225
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 225
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaaa ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcgcag ccgctgaact ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgatt atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 226
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 226
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Lys Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Gln Pro Leu Asn Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Asp Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 227
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 227
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaaa ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcgcag ccgctgaact ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgatt atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg cgggctacac ggcagtg 747
<210> 228
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 228
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Lys Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Gln Pro Leu Asn Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Asp Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Ala Gly Tyr Thr Ala Val
245
<210> 229
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 229
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaaa ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcgcag ccgctgaact ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaacatatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg agcggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgata ccggctacac ggcagtg 747
<210> 230
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 230
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Lys Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Gln Pro Leu Asn Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys His Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Ser Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Thr Gly Tyr Thr Ala Val
245
<210> 231
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 231
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcgtgg gcggtgaaaa ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcgcag ccgctgaact ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 232
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 232
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Val Gly Gly Glu
85 90 95
Lys Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Gln Pro Leu Asn Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 233
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 233
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaaa ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcgcag ccgctgaact ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatc cgggctacac ggcagtg 747
<210> 234
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 234
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Lys Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Gln Pro Leu Asn Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Pro Gly Tyr Thr Ala Val
245
<210> 235
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 235
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gaccatggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaaa ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtcta tcaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcgcag ccgctgaact ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgatt atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg aacggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 236
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 236
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp His Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Lys Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Ile Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Gln Pro Leu Asn Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Asp Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Asn Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 237
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 237
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtcag 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gacaagggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaaa ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtctg tgaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcgcag ccgctgaact ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 238
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 238
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Gln Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp Lys Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Lys Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Val Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Gln Pro Leu Asn Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 239
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 239
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaagcc gctaaagtca ttgtgtcaga tatcaacgaa 120
gacaagggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaaa ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtctg tgaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcgcag ccgctgaact ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 240
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 240
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Ala Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp Lys Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Lys Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Val Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Gln Pro Leu Asn Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 241
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 241
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gacaagggta ataaaaccgt tgaagatatt aaaagccagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaaa ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtctg tgaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcgcag ccgctgaact ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 242
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 242
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp Lys Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ser Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Lys Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Val Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Gln Pro Leu Asn Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 243
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 243
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gacaagggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac gcgggaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaaa ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtctg tgaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcgcag ccgctgaact ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 244
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 244
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp Lys Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Arg Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Lys Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Val Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Gln Pro Leu Asn Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 245
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 245
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gacaagggta ataaaaccgt tgaagatatt aaagcccaga acggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaaa ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtctg tgaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcgcag ccgctgaact ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 246
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 246
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp Lys Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Asn Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Lys Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Val Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Gln Pro Leu Asn Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 247
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 247
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gacaagggta ataaaaccgt tgaagatatt aaacaccagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaaa ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtctg tgaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcgcag ccgctgaact ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 248
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 248
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp Lys Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys His Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Lys Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Val Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Gln Pro Leu Asn Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 249
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 249
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gacaagggta ataaaaccgt tgaagatatt aaaggccagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaaa ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtctg tgaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcgcag ccgctgaact ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 250
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 250
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp Lys Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Gly Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Lys Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Val Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Gln Pro Leu Asn Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 251
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 251
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gacaagggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaaac 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaaa ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtctg tgaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcgcag ccgctgaact ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 252
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 252
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp Lys Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Asn
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Lys Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Val Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Gln Pro Leu Asn Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 253
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 253
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gacaagggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaaag 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaaa ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtctg tgaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcgcag ccgctgaact ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 254
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 254
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp Lys Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Lys
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Lys Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Val Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Gln Pro Leu Asn Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 255
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 255
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gacaagggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaaa ggcactggct 300
ggtgattaca agctggacag ctggcgtaaa gttctgtctg tgaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcgcag ccgctgaact ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 256
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 256
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp Lys Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Lys Ala Leu Ala Gly Asp Tyr Lys Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Val Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Gln Pro Leu Asn Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 257
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 257
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gacaagggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaaa ggcactggct 300
ggtgattacc ggctggacag ctggcgtaaa gttctgtctg tgaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcgcag ccgctgaact ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 258
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 258
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp Lys Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Lys Ala Leu Ala Gly Asp Tyr Arg Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Val Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Gln Pro Leu Asn Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 259
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 259
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gacaagggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaaa ggcactggct 300
ggtgattacc cgctggacag ctggcgtaaa gttctgtctg tgaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcgcag ccgctgaact ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 260
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 260
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp Lys Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Lys Ala Leu Ala Gly Asp Tyr Pro Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Val Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Gln Pro Leu Asn Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 261
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 261
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gacaagggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaaa ggcactggct 300
ggtgattaca ccctggacag ctggcgtaaa gttctgtctg tgaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcgcag ccgctgaact ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 262
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 262
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp Lys Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Lys Ala Leu Ala Gly Asp Tyr Thr Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Val Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Gln Pro Leu Asn Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 263
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 263
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gacaagggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaaa ggcactggct 300
ggtgattacc acctggacag ctggcgtaaa gttctgtctg tgaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcgcag ccgctgaact ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 264
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 264
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp Lys Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Lys Ala Leu Ala Gly Asp Tyr His Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Val Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Gln Pro Leu Asn Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 265
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 265
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gacaagggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaaa ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtctg tgaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaaca agggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcgcag ccgctgaact ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 266
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 266
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp Lys Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Lys Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Val Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Lys Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Gln Pro Leu Asn Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 267
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 267
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gacaagggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaaa ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtctg tgaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacc acggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcgcag ccgctgaact ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 268
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 268
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp Lys Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Lys Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Val Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn His Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Gln Pro Leu Asn Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 269
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 269
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gacaagggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaaa ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtctg tgaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaaca ccggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcgcag ccgctgaact ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 270
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 270
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp Lys Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Lys Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Val Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Thr Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Gln Pro Leu Asn Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 271
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 271
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gacaagggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaaa ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtctg tgaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacc gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcgcag ccgctgaact ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 272
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 272
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp Lys Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Lys Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Val Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Arg Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Gln Pro Leu Asn Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 273
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 273
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gacaagggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaaa ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtctg tgaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcgcag ccgctgaact ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
gcgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 274
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 274
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp Lys Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Lys Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Val Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Gln Pro Leu Asn Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Ala Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 275
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 275
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gacaagggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaaa ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtctg tgaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcgcag ccgctgaact ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
gagaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 276
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 276
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp Lys Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Lys Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Val Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Gln Pro Leu Asn Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Glu Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 277
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 277
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gacaagggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaaa ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtctg tgaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcgcag ccgctgaact ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag acctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 278
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 278
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp Lys Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Lys Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Val Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Gln Pro Leu Asn Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Asp Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 279
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 279
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gacaagggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaaa ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtctg tgaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcgcag ccgctgaact ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaaa acctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 280
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 280
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp Lys Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Lys Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Val Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Gln Pro Leu Asn Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Asn Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 281
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 281
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gacaagggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaaa ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtctg tgaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcgcag ccgctgaact ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctggcctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 282
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 282
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp Lys Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Lys Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Val Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Gln Pro Leu Asn Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ala Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 283
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 283
atgggtatcc tggacaacaa agtcgcagtg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gacaagggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaaa ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtctg tgaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcgcag ccgctgaact ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 284
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 284
Met Gly Ile Leu Asp Asn Lys Val Ala Val Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp Lys Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Lys Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Val Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Gln Pro Leu Asn Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 285
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 285
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcgtcggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gacaagggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaaa ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtctg tgaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcgcag ccgctgaact ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 286
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 286
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Ser Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp Lys Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Lys Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Val Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Gln Pro Leu Asn Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 287
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 287
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgcttcaga tatcaacgaa 120
gacaagggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaaa ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtctg tgaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcgcag ccgctgaact ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 288
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 288
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Ala Ser Asp Ile Asn Glu Asp Lys Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Lys Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Val Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Gln Pro Leu Asn Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 289
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 289
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gacaagggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagtttttcg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaaa ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtctg tgaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcgcag ccgctgaact ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 290
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 290
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp Lys Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Ser Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Lys Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Val Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Gln Pro Leu Asn Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 291
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 291
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gacaagggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttatg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaaa ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtctg tgaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcgcag ccgctgaact ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 292
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 292
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp Lys Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Met Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Lys Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Val Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Gln Pro Leu Asn Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 293
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 293
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gacaagggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccatgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaaa ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtctg tgaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcgcag ccgctgaact ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 294
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 294
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp Lys Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Met Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Lys Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Val Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Gln Pro Leu Asn Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 295
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 295
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gacaagggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaaa ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtctg tgaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atgtctagca ttcatggtat cgttgcgcag ccgctgaact ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 296
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 296
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp Lys Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Lys Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Val Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ser Ser Ile
130 135 140
His Gly Ile Val Ala Gln Pro Leu Asn Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 297
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 297
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gacaagggta ataaaaccgt tgaagatatt aaagcaaccg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaaa ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtctg tgaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcgcag ccgctgaact ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 298
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 298
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp Lys Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Thr Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Lys Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Val Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Gln Pro Leu Asn Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 299
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 299
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gacaagggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaaa ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtctg tgaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcgcag ccgctgaact ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaggcc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 300
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 300
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp Lys Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Lys Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Val Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Gln Pro Leu Asn Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Arg Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 301
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 301
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gacaagggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaaa ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtctg tgaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcgcag ccgctgaact ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc gagcgaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 302
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 302
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp Lys Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Lys Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Val Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Gln Pro Leu Asn Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Ser Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 303
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 303
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gacaagggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaaa ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtctg tgaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcgcag ccgctgaact ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgac accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 304
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 304
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp Lys Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Lys Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Val Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Gln Pro Leu Asn Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Asp Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 305
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 305
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gacaagggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaaa ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtctg tgaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcgcag ccgctgaact ctgcatatac ctctgcgaaa 480
cacgccgtga ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 306
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 306
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp Lys Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Lys Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Val Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Gln Pro Leu Asn Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Ile Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 307
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 307
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gacaagggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaaa ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtctg tgaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcgcag ccgctgaact ctgcatatac ctctgcgaaa 480
cacgccgtgc tgggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 308
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 308
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp Lys Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Lys Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Val Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Gln Pro Leu Asn Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Leu Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 309
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 309
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gacaagggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaaa ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtctg tgaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcgcag ccgctgaact ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcga cttgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 310
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 310
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp Lys Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Lys Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Val Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Gln Pro Leu Asn Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Thr Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 311
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 311
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gacaagggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaaa ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtctg tgaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcgcag ccgctgaact ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgt cgctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 312
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 312
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp Lys Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Lys Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Val Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Gln Pro Leu Asn Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Ser
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 313
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 313
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gacaagggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaaa ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtctg tgaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcgcag ccgctgaact ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactga cgctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 314
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 314
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp Lys Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Lys Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Val Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Gln Pro Leu Asn Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Thr
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 315
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 315
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gacaagggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaaa ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtctg tgaatctgga cggtgtcttc 360
tatggctgta aattttcgct ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcgcag ccgctgaact ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 316
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 316
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp Lys Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Lys Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Val Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Phe Ser Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Gln Pro Leu Asn Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 317
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 317
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gacaagggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaaa ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtctg tgaatctgga cggtgtcttc 360
tatggctgta aatttgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcgcag ccgctgaact ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 318
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 318
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp Lys Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Lys Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Val Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Phe Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Gln Pro Leu Asn Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 319
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 319
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gacaagggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaaa ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtctg tgaatctgga cggtgtcttc 360
tatggctgta aatacacgct ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcgcag ccgctgaact ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 320
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 320
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp Lys Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Lys Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Val Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Thr Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Gln Pro Leu Asn Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 321
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 321
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gacaagggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaaa ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtctg tgaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcgtg 420
atggccagca ttcatggtat cgttgcgcag ccgctgaact ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 322
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 322
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp Lys Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Lys Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Val Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Val Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Gln Pro Leu Asn Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 323
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 323
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gacaagggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaaa ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtctg tgaatctgga cggtgtcttc 360
tatggctgta aatacgctct ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcgcag ccgctgaact ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 324
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 324
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp Lys Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Lys Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Val Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Ala Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Gln Pro Leu Asn Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 325
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 325
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gacaagggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaaa ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtctg tgaatctgga cggtgtcttc 360
tatggctgta aatattctct ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcgcag ccgctgaact ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 326
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 326
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp Lys Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Lys Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Val Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Ser Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Gln Pro Leu Asn Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 327
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 327
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gacaagggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaaa ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtctg tgaatctgga cggtgtcttc 360
tatggctgta aatttgttct ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcgcag ccgctgaact ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgaat atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 328
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 328
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp Lys Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Lys Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Val Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Phe Val Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Gln Pro Leu Asn Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Glu Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 329
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 329
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gacaagggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg cgggtgaaaa ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtctg tgaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcgcag ccgctgaact ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgatt atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 330
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 330
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp Lys Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Ala Gly Glu
85 90 95
Lys Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Val Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Gln Pro Leu Asn Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Asp Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245
<210> 331
<211> 747
<212> DNA
<213> 人工序列(artificial sequence)
<400> 331
atgggtatcc tggacaacaa agtcgcactg gttacgggcg ctggttcggg catcggtctg 60
gcggtggcac actcctacgc taaagaaggc gctaaagtca ttgtgtcaga tatcaacgaa 120
gacaagggta ataaaaccgt tgaagatatt aaagcacagg gcggtgaagc tagttttgtg 180
aaagcggaca ccagcaaccc ggaagaagtg gaagccctgg ttaaacgtac ggtcgaaatt 240
tatggtcgcc tggatgtggc atgcaacaat gctggcattg gcggtgaaaa ggcactggct 300
ggtgattacg gcctggacag ctggcgtaaa gttctgtctg tgaatctgga cggtgtcttc 360
tatggctgta aatacgaact ggaacaaatg gagaaaaacg gcggtggcgt tatcgtcaat 420
atggccagca ttcatggtat cgttgcgcag ccgctgaact ctgcatatac ctctgcgaaa 480
cacgccgtgg ttggcctgac gaaaaacatt ggtgctgatt atggccagaa aaacatccgt 540
tgcaatgcgg tgtgcccggg ctacattgaa accccgctgc tggaatcact gacgaaagaa 600
atgaaagaag ccctgatctc gaaacacccg atgggtcgcc tgggcaaacc ggaagaagtg 660
gcagaactgg ttctgtttct gagttccgaa aaatcatcgt tcatgaccgg tggctattac 720
ctggtcgatg gtggctacac ggcagtg 747
<210> 332
<211> 249
<212> PRT
<213> 人工序列(artificial sequence)
<400> 332
Met Gly Ile Leu Asp Asn Lys Val Ala Leu Val Thr Gly Ala Gly Ser
1 5 10 15
Gly Ile Gly Leu Ala Val Ala His Ser Tyr Ala Lys Glu Gly Ala Lys
20 25 30
Val Ile Val Ser Asp Ile Asn Glu Asp Lys Gly Asn Lys Thr Val Glu
35 40 45
Asp Ile Lys Ala Gln Gly Gly Glu Ala Ser Phe Val Lys Ala Asp Thr
50 55 60
Ser Asn Pro Glu Glu Val Glu Ala Leu Val Lys Arg Thr Val Glu Ile
65 70 75 80
Tyr Gly Arg Leu Asp Val Ala Cys Asn Asn Ala Gly Ile Gly Gly Glu
85 90 95
Lys Ala Leu Ala Gly Asp Tyr Gly Leu Asp Ser Trp Arg Lys Val Leu
100 105 110
Ser Val Asn Leu Asp Gly Val Phe Tyr Gly Cys Lys Tyr Glu Leu Glu
115 120 125
Gln Met Glu Lys Asn Gly Gly Gly Val Ile Val Asn Met Ala Ser Ile
130 135 140
His Gly Ile Val Ala Gln Pro Leu Asn Ser Ala Tyr Thr Ser Ala Lys
145 150 155 160
His Ala Val Val Gly Leu Thr Lys Asn Ile Gly Ala Asp Tyr Gly Gln
165 170 175
Lys Asn Ile Arg Cys Asn Ala Val Cys Pro Gly Tyr Ile Glu Thr Pro
180 185 190
Leu Leu Glu Ser Leu Thr Lys Glu Met Lys Glu Ala Leu Ile Ser Lys
195 200 205
His Pro Met Gly Arg Leu Gly Lys Pro Glu Glu Val Ala Glu Leu Val
210 215 220
Leu Phe Leu Ser Ser Glu Lys Ser Ser Phe Met Thr Gly Gly Tyr Tyr
225 230 235 240
Leu Val Asp Gly Gly Tyr Thr Ala Val
245

Claims (22)

1.一种工程化酮还原酶多肽，所述酮还原酶多肽的氨基酸序列与SEQ ID NO:2的序列相比存在残基差异X185C，所述多肽的氨基酸序列为SEQ ID No：36、38、42、44、46、50、52、54、56、58、60、62、66、70、74、78、80、82、84、88、90、94、96、100、102、104、106、108、126、128、130、132、134、136、138、140、142、144、146、156、158、160、162、164、166、168、170、172、174、176、178、180、182、186、188、190、192、194、196、198、200、202、204、206、208、210、212、214、216、218、220、222、224、226、228、230、232、234、236、238、240、242、244、246、248、250、252、254、256、258、260、262、264、266、268、270、272、274、276、278、280、282、284、286、288、290、292、294、296、298、300、302、304、306、308、310、312、314、316、318、320、322、324、326、328、330、332所示的氨基酸序列。
2.一种通过化学键或物理吸附的方法被固定在固体材质上的多肽，所述多肽选自权利要求1所述的酮还原酶多肽。
3.一种多核苷酸，所述多核苷酸编码权利要求1的多肽。
4.如权利要求3所述的多核苷酸，其中所述多核苷酸序列为对应SEQ ID No：35、37、41、43、45、49、51、53、55、57、59、61、65、69、73、77、79、81、83、87、89、93、95、99、101、103、105、107、125、127、129、131、133、135、137、139、141、143、145、155、157、159、161、163、165、167、169、171、173、175、177、179、181、185、187、189、191、193、195、197、199、201、203、205、207、209、211、213、215、217、219、221、223、225、227、229、231、233、235、237、239、241、243、245、247、249、251、253、255、257、259、261、263、265、267、269、271、273、275、277、279、281、283、285、287、289、291、293、295、297、299、301、303、305、307、309、311、313、315、317、319、321、323、325、327、329、331所示的多核苷酸序列。
5.一种表达载体，所述表达载体包含权利要求3-4任一项所述的多核苷酸。
6.如权利要求5所述的表达载体，所述表达载体包括质粒、粘粒、噬菌体或病毒载体。
7.一种宿主细胞，所述宿主细胞包括权利要求5的表达载体，所述宿主细胞为大肠杆菌。
8.一种酮还原酶多肽的制备方法，其包括如下步骤：培养权利要求7所述的宿主细胞，以及从培养物中获得酮还原酶多肽。
9.一种酮还原酶催化剂，其选自权利要求8的培养物，通过从权利要求8的培养物中获得的含酮还原酶多肽的宿主细胞或培养液，或者用其加工的制品；其中，所述制品是指由权利要求7或8所述的宿主细胞得到的提取物，通过对所述提取物中的酮还原酶进行分离或纯化得到的分离产品，或通过固定化权利要求7或8所述的宿主细胞及其提取物或提取物的分离产品而得到的固定化制品。
10.一种制备结构式(I)化合物的方法：
Figure FDA0003313779580000011
所述结构式(I)的醇产物在标为*的手性中心具有所示的立体化学构型；所示结构式(I)的醇产物与相应的对映异构体相比过量，其中
R1是任选地取代的芳基或杂芳基，或是任选地取代的C1-C8烃基，也可以是环烃基或杂环基；R2是任选地取代的C1-C6烃基、卤素、烯基、炔基、芳基、杂芳基、-NO2、-NO、-SO2R’或-SOR’、-SR’、-NR’R’、-OR’、-CO2R’或-COR’、-CONR’、-CNR’、-SO2NH2或-SONH2、-CN、-CF3；其中每个R’独立地选自-H、卤素、C1-C8烃基、C2-C12烯基、C2-C12炔基、环烃基、芳基或杂环基，
所述方法包括，将结构式(II)的羰基底物：
Figure FDA0003313779580000021
与权利要求1的工程化多肽接触的步骤。
11.如权利要求10所述的方法，其中结构式(I)的化合物是
Figure FDA0003313779580000022
其中R2是任选地取代的C1-C6烃基、卤素、烯基、炔基、芳基、杂芳基、-NO2、-NO、-SO2R’或-SOR’、-SR’、-NR’R’、-OR’、-CO2R’或-COR’、-CONR’、-CNR’、-SO2NH2或-SONH2、-CN、-CF3；其中每个R’独立地选自-H、卤素、C1-C8烃基、C2-C12烯基、C2-C12炔基、环烃基、芳基或杂环基；R3或R4是任选地取代的C1-C4烃基、-H、卤素、芳基、杂芳基、-NO2、-NO、-SO2R’或-SOR’、-SR’、-NR’R’、-OR’、-CO2R’或-COR’、-CONR’、-CNR’、-SO2NH2或-SONH2、-CN、CF3，其中每个R’独立地选自-H、C1-C4烃基、环烃基、芳基或杂环基；
且结构式(II)的底物是：
Figure FDA0003313779580000023
12.如权利要求10所述的方法，其中结构式(I)的化合物是：
Figure FDA0003313779580000024
13.如权利要求11所述的方法，其中R3在苯基环的对位，或者R3在苯基环的间位，或者R3在苯基环的邻位；或者R3同时在苯基环的对位和间位，或者R3同时在苯基环的对位和邻位，或者R3同时在苯基环的间位和邻位。
14.一种制备式A2化合物(R)-(-)-1,3-丁二醇的方法：
Figure FDA0003313779580000031
所述方法包括，将底物即结构式A1的化合物4-羟基-2-丁酮
Figure FDA0003313779580000032
与权利要求1的工程化酮还原酶多肽接触的步骤。
15.如权利要求10-14任一项所述的方法，手性醇产物以至少90％的对映体过量产生。
16.如权利要求10-14任一项所述的方法，其中NADH在反应过程中是循环再生的，再生的方法或是通过酮还原酶将异丙醇转化为丙酮的过程来实现；或是通过葡萄糖脱氢酶将葡萄糖转化为葡萄糖酸的过程来实现；或是通过甲酸脱氢酶将甲酸转化为二氧化碳的过程来实现。
17.如权利要求10-14任一项所述的方法，反应溶剂包括水，甲醇，乙醇，丙醇，异丙醇，乙酸异丙酯，二甲基亚砜或二甲基甲酰胺。
18.如权利要求10-14任一项所述的方法，其中，反应体系的温度为10℃至60℃。
19.如权利要求10-14任一项所述的方法，其中，反应体系的pH为pH4.0至pH11.0。
20.如权利要求10-14任一项所述的方法，底物载量为5g/L至400g/L。
21.一种拆分外消旋的1,3-丁二醇的方法，所述方法包括，将外消旋的1,3-丁二醇与权利要求1的工程化多肽接触的步骤。
22.如权利要求21所述的方法，反应条件包括5g/L-400g/L的外消旋的1,3-丁二醇，0.01g/L-0.2g/L的NAD+，10％-50％v/v的丙酮，反应温度10-60℃。
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