CN111317718A - 一种虫草素多重自乳化给药体系的制备 - Google Patents

一种虫草素多重自乳化给药体系的制备 Download PDF

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CN111317718A
CN111317718A CN201811404285.XA CN201811404285A CN111317718A CN 111317718 A CN111317718 A CN 111317718A CN 201811404285 A CN201811404285 A CN 201811404285A CN 111317718 A CN111317718 A CN 111317718A
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cordycepin
stirring
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吴晓琼
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Nanjing Polytechnic Institute
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Nanjing Polytechnic Institute
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/10—Dispersions; Emulsions
    • A61K9/107—Emulsions ; Emulsion preconcentrates; Micelles
    • A61K9/113—Multiple emulsions, e.g. oil-in-water-in-oil
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/7042—Compounds having saccharide radicals and heterocyclic rings
    • A61K31/7052—Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides
    • A61K31/706—Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides containing six-membered rings with nitrogen as a ring hetero atom
    • A61K31/7064—Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides containing six-membered rings with nitrogen as a ring hetero atom containing condensed or non-condensed pyrimidines
    • A61K31/7076—Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides containing six-membered rings with nitrogen as a ring hetero atom containing condensed or non-condensed pyrimidines containing purines, e.g. adenosine, adenylic acid
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
    • A61K47/26—Carbohydrates, e.g. sugar alcohols, amino sugars, nucleic acids, mono-, di- or oligo-saccharides; Derivatives thereof, e.g. polysorbates, sorbitan fatty acid esters or glycyrrhizin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
    • A61K47/42—Proteins; Polypeptides; Degradation products thereof; Derivatives thereof, e.g. albumin, gelatin or zein
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P39/00—General protective or antinoxious agents
    • A61P39/06—Free radical scavengers or antioxidants

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Abstract

本发明涉及天然活性物虫草素多重自乳化体系的制备，通过二步乳化法制备虫草素多重自乳化体系，MCT、PGPR、GMS、甘油在65℃下混合混匀形成油相，同温度下去离子水、明胶、虫草素混合形成水相，将水相加入油相550r/min搅拌15分钟，得到初乳。初乳冷却至50℃时，加入吐温80，250r/min搅拌15分钟得到复乳。

Description

一种虫草素多重自乳化给药体系的制备
技术领域
发明涉及天然活性物虫草素载体的制备。
背景技术
虫草素是冬虫夏草的活性成分之一，具有抗衰老、抗肿瘤、抗菌等作用，目前主要应用于保健品中，但虫草素脂溶性较差、不易被人体吸收，高温活性成分易被破坏，生物利用率较低，限制了虫草素的应用。
因此制备合适的虫草素的载体十分重要，目前针对虫草素多重乳液的制备的研究未见报道。
发明内容
本发明研究的制备工艺，涉及到多重乳液的制备配方以及工艺。
本发明目的是通过以下技术方案来实现:
MCT、PGPR、GMS、甘油在65℃下混合混匀形成油相，同温度下去离子水、明胶、虫草素混合形成水相，将水相加入油相550r/min搅拌15分钟，得到初乳。初乳冷却至50℃时，加入吐温80，250r/min搅拌15分钟得到复乳。
其中MCT：GMS：PGPR：甘油=40:10:10:2，水相与油相比例为7:3。
具体实施方式
本发明结合实例做进一步描述，但并不是限制本发明。
实施例1：
1.仪器与试剂
电子分析天平BS124S 型（北京赛多利斯仪器系统有限公司）、磁力搅拌电热恒温水浴锅EMS-30 型（上海乔跃电子有限公司）、电热恒温鼓风干燥箱DHG-9030A 型（上海精宏试验设备有限公司）
虫草素（98%）（陕西柏科生物科技有限公司）、辛癸酸甘油酯（河南正通化工有限公司）、聚氧乙烯脱水山梨醇单油酸酯（国药集团化学试剂有限公司）、丙三醇(国药集团化学试剂有限公司)、聚甘油蓖麻醇脂（上海佑创实业有限公司）、单硬脂酸甘油酯（莘县新星油脂有限公司）
2.制备
MCT、PGPR、GMS、甘油在65℃下混合混匀形成油相，同温度下去离子水、明胶、虫草素混合形成水相，将水相加入油相550r/min搅拌15分钟，得到初乳。初乳冷却至50℃时，加入吐温80，250r/min搅拌15分钟得到复乳。
其中MCT为4.0g、 GMS为1.0 g、吐温80为0.6g、PGPR为1g、甘油0.2 g、水相6.4 g，虫草素负载量0.02 g。
3.表征
光学显微镜观察其形貌，同为时考察其稳定性。
实施例2：
如实例1所述，选择虫草素负载量0.04 g。

Claims (2)

1.一种虫草素多重自乳化给药的制备，通过二步乳化法制备虫草素多重乳液，将MCT、GMS、PGPR、甘油在65℃下机械搅拌混合形成油相，同温度下虫草素、去离子水、明胶混合形成水相，将水相加入油相磁子搅拌15分钟得到初乳，取一定量的初乳和吐温80混合均匀，明胶完全溶解在水中后，将水相加入初乳，磁子搅拌15分钟得到复乳。
2.根据权利要求1，其中MCT：GMS：PGPR：甘油=40:10:10:2，水相与油相比例为7:3。
CN201811404285.XA 2018-11-23 2018-11-23 一种虫草素多重自乳化给药体系的制备 Pending CN111317718A (zh)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115381742A (zh) * 2022-09-01 2022-11-25 南京科技职业学院 一种虫草素多重乳液、水凝胶球及制备方法

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115381742A (zh) * 2022-09-01 2022-11-25 南京科技职业学院 一种虫草素多重乳液、水凝胶球及制备方法

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