CN111315212B - 经过基因组编辑的鸟 - Google Patents

经过基因组编辑的鸟 Download PDF

Info

Publication number
CN111315212B
CN111315212B CN201880064933.3A CN201880064933A CN111315212B CN 111315212 B CN111315212 B CN 111315212B CN 201880064933 A CN201880064933 A CN 201880064933A CN 111315212 B CN111315212 B CN 111315212B
Authority
CN
China
Prior art keywords
bird
chromosome
dna
cells
nucleic acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201880064933.3A
Other languages
English (en)
Chinese (zh)
Other versions
CN111315212A (zh
Inventor
Y·西纳蒙
E·本塔尔科恩
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Agricultural Research Organization of Israel Ministry of Agriculture
Original Assignee
Agricultural Research Organization of Israel Ministry of Agriculture
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agricultural Research Organization of Israel Ministry of Agriculture filed Critical Agricultural Research Organization of Israel Ministry of Agriculture
Priority to CN202210600684.3A priority Critical patent/CN114958913A/zh
Publication of CN111315212A publication Critical patent/CN111315212A/zh
Application granted granted Critical
Publication of CN111315212B publication Critical patent/CN111315212B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027New or modified breeds of vertebrates
    • A01K67/0275Genetically modified vertebrates, e.g. transgenic
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90Stable introduction of foreign DNA into chromosome
    • C12N15/902Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0608Germ cells
    • C12N5/0611Primordial germ cells, e.g. embryonic germ cells [EG]
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00Animals characterised by species
    • A01K2227/30Bird
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00Animals characterised by purpose
    • A01K2267/02Animal zootechnically ameliorated
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00Genetically modified cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00Nucleic acids vectors
    • C12N2800/10Plasmid DNA
    • C12N2800/106Plasmid DNA for vertebrates

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Environmental Sciences (AREA)
  • Molecular Biology (AREA)
  • Veterinary Medicine (AREA)
  • Developmental Biology & Embryology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Animal Behavior & Ethology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
CN201880064933.3A 2017-09-19 2018-09-17 经过基因组编辑的鸟 Active CN111315212B (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202210600684.3A CN114958913A (zh) 2017-09-19 2018-09-17 经过基因组编辑的鸟

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201762560218P 2017-09-19 2017-09-19
US62/560,218 2017-09-19
PCT/IL2018/051056 WO2019058376A1 (en) 2017-09-19 2018-09-17 BIRDS WITH GENOME EDITION

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CN202210600684.3A Division CN114958913A (zh) 2017-09-19 2018-09-17 经过基因组编辑的鸟

Publications (2)

Publication Number Publication Date
CN111315212A CN111315212A (zh) 2020-06-19
CN111315212B true CN111315212B (zh) 2022-06-14

Family

ID=65811120

Family Applications (2)

Application Number Title Priority Date Filing Date
CN201880064933.3A Active CN111315212B (zh) 2017-09-19 2018-09-17 经过基因组编辑的鸟
CN202210600684.3A Pending CN114958913A (zh) 2017-09-19 2018-09-17 经过基因组编辑的鸟

Family Applications After (1)

Application Number Title Priority Date Filing Date
CN202210600684.3A Pending CN114958913A (zh) 2017-09-19 2018-09-17 经过基因组编辑的鸟

Country Status (12)

Country Link
US (1) US20200214273A1 (enExample)
EP (1) EP3684172A4 (enExample)
JP (1) JP2020536580A (enExample)
KR (1) KR20200088805A (enExample)
CN (2) CN111315212B (enExample)
AU (1) AU2018336161A1 (enExample)
BR (1) BR112020005272A2 (enExample)
CA (1) CA3075956A1 (enExample)
EA (1) EA202090585A1 (enExample)
IL (1) IL273190A (enExample)
MX (1) MX2020003123A (enExample)
WO (1) WO2019058376A1 (enExample)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2020232441A1 (en) * 2019-03-05 2021-09-23 The State Of Israel, Ministry Of Agriculture & Rural Development, Agricultural Research Organization (Aro) (Volcani Center) Genome-edited birds
CN112522315B (zh) * 2020-12-08 2023-04-11 广东省农业科学院动物科学研究所 一种鸡原始生殖细胞转染方法
TW202241259A (zh) * 2020-12-31 2022-11-01 以色列國家農業部、農村發展農業研究組織(沃爾卡尼研究所) 不育禽類胚胎、其產生及用途

Family Cites Families (47)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL154600B (nl) 1971-02-10 1977-09-15 Organon Nv Werkwijze voor het aantonen en bepalen van specifiek bindende eiwitten en hun corresponderende bindbare stoffen.
NL154598B (nl) 1970-11-10 1977-09-15 Organon Nv Werkwijze voor het aantonen en bepalen van laagmoleculire verbindingen en van eiwitten die deze verbindingen specifiek kunnen binden, alsmede testverpakking.
NL154599B (nl) 1970-12-28 1977-09-15 Organon Nv Werkwijze voor het aantonen en bepalen van specifiek bindende eiwitten en hun corresponderende bindbare stoffen, alsmede testverpakking.
US3901654A (en) 1971-06-21 1975-08-26 Biological Developments Receptor assays of biologically active compounds employing biologically specific receptors
US3853987A (en) 1971-09-01 1974-12-10 W Dreyer Immunological reagent and radioimmuno assay
US3867517A (en) 1971-12-21 1975-02-18 Abbott Lab Direct radioimmunoassay for antigens and their antibodies
NL171930C (nl) 1972-05-11 1983-06-01 Akzo Nv Werkwijze voor het aantonen en bepalen van haptenen, alsmede testverpakkingen.
US3850578A (en) 1973-03-12 1974-11-26 H Mcconnell Process for assaying for biologically active molecules
US3935074A (en) 1973-12-17 1976-01-27 Syva Company Antibody steric hindrance immunoassay with two antibodies
US3996345A (en) 1974-08-12 1976-12-07 Syva Company Fluorescence quenching with immunological pairs in immunoassays
US4034074A (en) 1974-09-19 1977-07-05 The Board Of Trustees Of Leland Stanford Junior University Universal reagent 2-site immunoradiometric assay using labelled anti (IgG)
US3984533A (en) 1975-11-13 1976-10-05 General Electric Company Electrophoretic method of detecting antigen-antibody reaction
US4098876A (en) 1976-10-26 1978-07-04 Corning Glass Works Reverse sandwich immunoassay
US4879219A (en) 1980-09-19 1989-11-07 General Hospital Corporation Immunoassay utilizing monoclonal high affinity IgM antibodies
US5011771A (en) 1984-04-12 1991-04-30 The General Hospital Corporation Multiepitopic immunometric assay
US4666828A (en) 1984-08-15 1987-05-19 The General Hospital Corporation Test for Huntington's disease
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4801531A (en) 1985-04-17 1989-01-31 Biotechnology Research Partners, Ltd. Apo AI/CIII genomic polymorphisms predictive of atherosclerosis
US5272057A (en) 1988-10-14 1993-12-21 Georgetown University Method of detecting a predisposition to cancer by the use of restriction fragment length polymorphism of the gene for human poly (ADP-ribose) polymerase
US5192659A (en) 1989-08-25 1993-03-09 Genetype Ag Intron sequence analysis method for detection of adjacent and remote locus alleles as haplotypes
US5281521A (en) 1992-07-20 1994-01-25 The Trustees Of The University Of Pennsylvania Modified avidin-biotin technique
US5944710A (en) 1996-06-24 1999-08-31 Genetronics, Inc. Electroporation-mediated intravascular delivery
US6774279B2 (en) 1997-05-30 2004-08-10 Carnegie Institution Of Washington Use of FLP recombinase in mice
EP1007633B1 (en) 1997-08-04 2005-10-19 University of Massachusetts, a Public Institution of Higher Education of The Commonwealth of Massachusetts, Avian primordial germ cell (pgc) cell line and a method for long term culturing thereof
GB2355459B (en) * 1999-11-29 2001-09-26 Isis Innovation A dominant conditional lethal genetic system
FR2810048A1 (fr) * 2000-06-07 2001-12-14 Centre Nat Rech Scient Construction d'acide nucleique utile pour l'expression de transgenes notamment dans les cellules souches embryonnaires
ATE513038T1 (de) * 2001-09-18 2011-07-15 Synageva Biopharma Corp Produktion eines transgenen vogels mittels cytoplasmainjektion
AU2002341795A1 (en) * 2001-09-21 2003-04-01 Avigenics, Inc. Production of transgenic avians using sperm-mediated transfection
CN1141391C (zh) * 2001-11-02 2004-03-10 浙江省农业科学院 家蚕性连锁平衡致死系性别控制基因的导入方法
EP1504092B2 (en) 2002-03-21 2014-06-25 Sangamo BioSciences, Inc. Methods and compositions for using zinc finger endonucleases to enhance homologous recombination
DE10248361A1 (de) * 2002-06-30 2004-01-22 Beisswanger, Roland, Dr. Unterdrückung eines Geschlechtes durch die Veränderung von Geschlechtschromosomen
US9447434B2 (en) 2002-09-05 2016-09-20 California Institute Of Technology Use of chimeric nucleases to stimulate gene targeting
CA2513744C (en) 2003-01-16 2013-12-24 North Carolina State University Depletion of endogenous primordial germ cells in avian species
US20060014264A1 (en) 2004-07-13 2006-01-19 Stowers Institute For Medical Research Cre/lox system with lox sites having an extended spacer region
DK2628794T3 (en) 2005-10-18 2016-08-15 Prec Biosciences RATIONALE CONSTRUCTED MECHANUCLEAS WITH CHANGED SEQUENCE SPECIFICITY AND DNA BINDING EFFICIENCY
WO2009023800A1 (en) * 2007-08-14 2009-02-19 Origen Therapeutics Transgenic chickens with an inactivated endogenous gene locus
EP2067402A1 (en) 2007-12-07 2009-06-10 Max Delbrück Centrum für Molekulare Medizin (MDC) Berlin-Buch; Transponson-mediated mutagenesis in spermatogonial stem cells
RU2011129621A (ru) * 2008-12-17 2013-01-27 Коммонвелт Сайентифик Энд Индастриал Рисерч Организейшн Способы модуляции пола птиц
WO2010088742A1 (en) * 2009-02-08 2010-08-12 The University Of Melbourne Sex-determination and methods of specifying same
EP2571512B1 (en) 2010-05-17 2017-08-23 Sangamo BioSciences, Inc. Novel dna-binding proteins and uses thereof
KR20230065381A (ko) 2012-07-25 2023-05-11 더 브로드 인스티튜트, 인코퍼레이티드 유도 dna 결합 단백질 및 게놈 교란 도구 및 이의 적용
PT3502240T (pt) 2012-11-27 2021-08-11 Childrens Medical Ct Corp Elementos reguladores distais de bcl11a como alvo para a reindução de hemoglobina fetal
US8697359B1 (en) 2012-12-12 2014-04-15 The Broad Institute, Inc. CRISPR-Cas systems and methods for altering expression of gene products
WO2015199225A1 (ja) * 2014-06-27 2015-12-30 国立研究開発法人産業技術総合研究所 家禽始原生殖細胞の遺伝子改変方法、遺伝子改変された家禽始原生殖細胞、遺伝子改変家禽の生産方法及び家禽卵
KR20180099704A (ko) * 2015-12-03 2018-09-05 이지지엑스와이티 리미티드 부화되지 않은 알에서 조류 배아의 성별 결정 방법 및 이의 수단
CN106359073A (zh) * 2016-08-23 2017-02-01 吉林省农业科学院 孤雄诱导基因表达盒和孤雄诱导表达载体及其应用
AU2020232441A1 (en) * 2019-03-05 2021-09-23 The State Of Israel, Ministry Of Agriculture & Rural Development, Agricultural Research Organization (Aro) (Volcani Center) Genome-edited birds

Also Published As

Publication number Publication date
US20200214273A1 (en) 2020-07-09
JP2020536580A (ja) 2020-12-17
EP3684172A4 (en) 2021-06-23
EA202090585A1 (ru) 2020-07-08
CN114958913A (zh) 2022-08-30
BR112020005272A2 (pt) 2020-11-17
AU2018336161A1 (en) 2020-03-26
CA3075956A1 (en) 2019-03-28
CN111315212A (zh) 2020-06-19
MX2020003123A (es) 2020-09-14
KR20200088805A (ko) 2020-07-23
IL273190A (en) 2020-04-30
EP3684172A1 (en) 2020-07-29
WO2019058376A1 (en) 2019-03-28

Similar Documents

Publication Publication Date Title
KR101982360B1 (ko) 콤팩트 tale-뉴클레아제의 발생 방법 및 이의 용도
US11667890B2 (en) Engineered artificial antigen presenting cells for tumor infiltrating lymphocyte expansion
CN113692225B (zh) 经基因组编辑的鸟类
DK2718440T3 (en) NUCLEASE ACTIVITY PROTEIN, FUSION PROTEINS AND APPLICATIONS THEREOF
US20040003420A1 (en) Modified recombinase
KR102516697B1 (ko) 조작된 캐스케이드 구성성분 및 캐스케이드 복합체
KR20210149060A (ko) Tn7-유사 트랜스포존을 사용한 rna-유도된 dna 통합
DK2663645T3 (da) Gærstammer, der er modificeret til produktion af ethanol fra glycerol
CN108431225A (zh) 细胞基因组的诱导型修饰
KR20150125994A (ko) 세포 발현 시스템
PT1984512T (pt) Sistema de expressão génica utilizando excisão-união em insetos
KR102584628B1 (ko) T-세포 수용체, t-세포 항원 및 이들의 기능성 상호작용의 식별 및 특징규명을 위한 조작된 다성분 시스템
KR20240029020A (ko) Dna 변형을 위한 crispr-트랜스포손 시스템
CN114585392A (zh) 通过抑制linc复合物治疗/预防疾病
CN111315212B (zh) 经过基因组编辑的鸟
CN111094569A (zh) 光控性病毒蛋白质、其基因及包含该基因的病毒载体
KR20230131229A (ko) 부위 특이적 유전자 변형
CN112877292A (zh) 产生人抗体的细胞
CN116323942A (zh) 用于基因组编辑的组合物及其使用方法
EP1395612A2 (en) Modified recombinase
KR20140043890A (ko) 조절된 유전자 발현 시스템 및 그의 작제물
CA2395490A1 (en) Repressible sterility of animals
CN116457465A (zh) 用于基因组修饰的方法和组合物
CN114958758B (zh) 一种乳腺癌模型猪的构建方法及应用
AU782109B2 (en) Repressible sterility of animals

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant